Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0003681

Physical Location & Seq

Genomic Coordinates Reverse (-)

6634716 .. 6636955

Gene Structure 2240 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g12134.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGACAGTGCCACGTGGTATTCCGGGTACAAAGAATAATTACTCTGAAGAAAAAGGTTGGATGGCTTATCCGATCGCGAATTTGCAAGACTTGATCCCCCCAACAATGTCTTCCTTTTTCTCCGCCTATGCTCCCTATGCTGGTTACGTGTTTATGCTTCAAGCATCATTCAAATACTTGAAATCAAGCATCAACTACCTCCTAGACCCTGAAGCTAATCATATGACTCTCCTCATCGACGTTTGTGGCCAACGCGGAATGACTCAGCGCAACCAAGTCTATGATGCAGCTGAGGTATATCTCGCCACCAAGATCAGCCCTAAAACCCTAGTTTTTCAAACCACCAAAACACCTGAACAAAAGACCCCCAGACTTGCCGTTACCACCACTCAAGAAATCACCGACACTTTCGAAAACATTAAACTAAAATGGCGCATGATCTTCCCTTCCGATGATGACCATCACTGCAGTAGTGATCTGAGTCCTCGCATTGAGCTAACATTCCACAAGAAACACAAGGACAAGGTGATGGACTCGTACATCCCCTATGTGTTGGCTCAGGCTGAGGCTATTAAAGACGCAGAGGCTATGAATGATGACGACAAGAATCTGAAGCTTTATAGCTACAACGTCAAACGTTCCATTCAAAACCAGACTACTCGTGGCAATTGGAGCTGCGTAGACCTAGAGCACCCAGCTACTTTCGAAACAATAGCAATGGAGCCGGAGCTGAAGAGGATGATCATAGAGGATTTGGACAAGTTTATGAAGAGGAGGGAGTTCTATAAGAGAGTTGGCAAACCTTGGAAGAGAGGTTACTTGTTGTATGGTCCACCCGGTACTGGAAAATCCAGCTTGGTTGCTGCCATGGCTAATCACCTCCATTTTAACATTTACGACTTGGAACTTACTAGTTTTTCCAGCAATGAAGAGTTGAGGAAAGCCTTGATGTCTACCACAAATCGTTCTATTGTCGTGTTTGAGGATATTGATTGCATCGAGGGATCCATAAACCGGGAATCAACAGAGAACAAGAAGTTTAGGGTTTCCGAAGCCAAGGTTACACTCTCGGGTCTACTGAATGTCCTCGATGGTCTGTGGTCAAGTTGCGGTGATGAGAGGATTGTGGTGTTAACCACCAACCACAAGGAACGGCTAGACACTGCATTGTTGCGGCCTGGTCGAATGGACATGCACATTCACATGCCGTATTGCACCCCAAGAGGTTTTGAGACCTTAGCCTCGAACTACCTTGGCATTCAGGACACCGGAGAACGTCACCTGGGTCAAGAAATTGAAGGTTTGATGGAGAGCACAAACATCACCCCTGCAGAGGTTTGTGAGGAGCTTATGAAGGGTGAGGGTGAGGGTGATGCTGCTCTTGAAGGTGTTGTCAATTTACTCAAACGCAAGAGGGAAGAAAGCAAAGTAATAGTGATCGAGGATGAAAGGAACAAAGAACCTGAAATTCCAAACGCAATTGCAAAGAGGCAGAAAACAAAGGATAACAACATAAGCAAATTTTTCAGAAAGGTGAGTAAGAAAATTGGAATTTCTCGAGTTAGAAATGGAAAACGAAGAAGACCGGGTTGGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006996 GO:0007005 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017004 GO:0017062 GO:0017111 GO:0019866 GO:0022607 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0033108 GO:0034551 GO:0034622 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA AAA_assoc

Protein Analysis

533

Amino Acids

60.79

Weight (kDa)

8.42

Isoelectric Point (pI)

50.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_assoc	PF14363	58 - 146	4.7e-14	Domain associated at C-terminal with AAA
AAA	PF00004	273 - 404	3.5e-21	ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540	PF25568	405 - 474	4.6e-16	At3g28540-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003681)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g12131 FvH4_1g12133 FvH4_1g12134
malus_domestica	MD15G1253100.v1.1 MD15G1253200.v1.1 MD15G1253400.v1.1 MD15G1254000.v1.1
rosa_chinensis	RchiOBHm_Chr2g0099811 RchiOBHm_Chr2g0099831 RchiOBHm_Chr2g0099841
rosa_laevigata	RLG00000016916 RLG00000016917 RLG00000016918
rosa_multiflora	Rmu_co8276881.1_g000001 Rmu_sc0004158.1_g000001 Rmu_sc0004158.1_g000002 Rmu_sc0004158.1_g000003 Rmu_sc0004158.1_g000004 Rmu_sc0008864.1_g000006 Rmu_sc0008864.1_g000007
rosa_roxburghii	Rroxscaffold_2G00142760
rosa_rugosa	Rorug02G0083400.1 Rorug02G0083500
rosa_samantha	Rh2BG135700 Rh2BG135900 Rh2BG136000 Rh2CG137300 Rh2CG137500 Rh2CG137600 Rh2DG137700 Rh2DG137900 Rh2DG138000
rosa_wichuraiana	Rw2G010280 Rw2G010300 Rw2G010310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	681, 953, 1075
AccII	CGCG	2 cut(s)	77, 255
AciI	CCGC	4 cut(s)	123, 255, 1110, 1174
AclI	AACGTT	1 cut(s)	637
AclWI	GGATC	3 cut(s)	88, 999, 1012
AcoI	YGGCCR	2 cut(s)	247, 1592
AcsI	RAATTY	4 cut(s)	79, 1467, 1520, 1551
AcuI	CTGAAG	4 cut(s)	66, 231, 632, 752
AcvI	CACGTG	1 cut(s)	14
AfaI	GTAC	3 cut(s)	28, 539, 841
AfiI	CCNNNNNNNGG	4 cut(s)	140, 842, 1014, 1333
AflIII	ACRYGT	1 cut(s)	147
AgsI	TTSAA	7 cut(s)	161, 172, 181, 338, 647, 1298, 1385
AhlI	ACTAGT	1 cut(s)	911
AjnI	CCWGG	2 cut(s)	1177, 1281
AjuI	GAANNNNNNNTTGG	3 cut(s)	94, 126, 1573
Alw21I	GWGCWC	2 cut(s)	693, 1316
Alw26I	GTCTC	1 cut(s)	1226
AlwI	GGATC	3 cut(s)	88, 999, 1012
Ama87I	CYCGRG	2 cut(s)	1069, 1557
AoxI	GGCC	3 cut(s)	247, 1175, 1592
ApeKI	GCWGC	4 cut(s)	287, 675, 863, 1376
ApoI	RAATTY	4 cut(s)	79, 1467, 1520, 1551
AspLEI	GCGC	2 cut(s)	270, 435
AspS9I	GGNCC	1 cut(s)	830
AsuC2I	CCSGG	4 cut(s)	24, 837, 1016, 1587
AsuII	TTCGAA	2 cut(s)	411, 705
AvaI	CYCGRG	2 cut(s)	1069, 1557
AvaII	GGWCC	1 cut(s)	830
BalI	TGGCCA	2 cut(s)	249, 1594
BamHI	GGATCC	1 cut(s)	1004
BarI	GAAGNNNNNNTAC	2 cut(s)	800, 832
BauI	CACGAG	1 cut(s)	660
BbrPI	CACGTG	1 cut(s)	14
BbsI	GAAGAC	2 cut(s)	102, 1588
Bbv12I	GWGCWC	2 cut(s)	693, 1316
BbvCI	CCTCAGC	2 cut(s)	291, 564
BbvI	GCAGC	4 cut(s)	299, 662, 850, 1363
BccI	CCATC	5 cut(s)	55, 468, 523, 1085, 1300
BceAI	ACGGC	3 cut(s)	362, 1169, 1192
BciT130I	CCWGG	2 cut(s)	1179, 1283
BclI	TGATCA	1 cut(s)	741
BcnI	CCSGG	4 cut(s)	24, 837, 1016, 1587
BcoDI	GTCTC	1 cut(s)	1226
BcuI	ACTAGT	1 cut(s)	911
BfaI	CTAG	5 cut(s)	203, 329, 686, 912, 1157
BfmI	CTRYAG	2 cut(s)	466, 1329
BisI	GCNGC	5 cut(s)	288, 676, 864, 1175, 1377
BlsI	GCNGC	5 cut(s)	289, 677, 865, 1176, 1378
Bme1390I	CCNGG	6 cut(s)	24, 837, 1016, 1179, 1283, 1587
Bme18I	GGWCC	1 cut(s)	830
BmeT110I	CYCGRG	2 cut(s)	1069, 1557
BmgT120I	GGNCC	1 cut(s)	830
BmiI	GGNNCC	2 cut(s)	723, 1006
BmrFI	CCNGG	6 cut(s)	24, 837, 1016, 1179, 1283, 1587
BmsI	GCATC	5 cut(s)	173, 198, 274, 1005, 1363
BpiI	GAAGAC	2 cut(s)	102, 1588
Bpu10I	CCTNAGC	4 cut(s)	291, 558, 564, 1237
Bpu14I	TTCGAA	2 cut(s)	411, 705
BpuEI	CTTGAG	1 cut(s)	375
BpuMI	CCSGG	4 cut(s)	24, 837, 1016, 1587
BsaAI	YACGTR	2 cut(s)	14, 148
BsaBI	GATNNNNATC	1 cut(s)	459
BsaI	GGTCTC	1 cut(s)	1226
BsaJI	CCNNGG	5 cut(s)	803, 867, 1056, 1252, 1282
BsaWI	WCCGGW	1 cut(s)	1268
Bsc4I	CCNNNNNNNGG	4 cut(s)	140, 842, 1014, 1333
Bse1I	ACTGG	1 cut(s)	847
Bse3DI	GCAATG	2 cut(s)	723, 931
Bse8I	GATNNNNATC	1 cut(s)	459
BseBI	CCWGG	2 cut(s)	1179, 1283
BseDI	CCNNGG	5 cut(s)	803, 867, 1056, 1252, 1282
BseGI	GGATG	4 cut(s)	66, 540, 744, 1450
BseJI	GATNNNNATC	1 cut(s)	459
BseLI	CCNNNNNNNGG	4 cut(s)	140, 842, 1014, 1333
BseMI	GCAATG	2 cut(s)	723, 931
BseMII	CTCAG	5 cut(s)	278, 282, 470, 555, 572
BseNI	ACTGG	1 cut(s)	847
BseRI	GAGGAG	3 cut(s)	221, 787, 1358
BseXI	GCAGC	4 cut(s)	299, 662, 850, 1363
BseYI	CCCAGC	1 cut(s)	694
Bsh1236I	CGCG	2 cut(s)	77, 255
Bsh1285I	CGRYCG	1 cut(s)	75
BshFI	GGCC	3 cut(s)	249, 1177, 1594
BsiEI	CGRYCG	1 cut(s)	75
BsiHKAI	GWGCWC	2 cut(s)	693, 1316
BsiHKCI	CYCGRG	2 cut(s)	1069, 1557
BsiSI	CCGG	6 cut(s)	23, 725, 837, 1015, 1269, 1586
BslI	CCNNNNNNNGG	4 cut(s)	140, 842, 1014, 1333
BsmAI	GTCTC	1 cut(s)	1226
BsmI	GAATGC	1 cut(s)	1257
BsnI	GGCC	3 cut(s)	249, 1177, 1594
Bso31I	GGTCTC	1 cut(s)	1226
BsoBI	CYCGRG	2 cut(s)	1069, 1557
Bsp119I	TTCGAA	2 cut(s)	411, 705
Bsp1286I	GDGCHC	2 cut(s)	693, 1316
Bsp143I	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
Bsp19I	CCATGG	1 cut(s)	867
Bsp68I	TCGCGA	1 cut(s)	77
BspACI	CCGC	4 cut(s)	123, 255, 1110, 1174
BspANI	GGCC	3 cut(s)	249, 1177, 1594
BspCNI	CTCAG	5 cut(s)	277, 283, 471, 556, 571
BspFNI	CGCG	2 cut(s)	77, 255
BspLI	GGNNCC	2 cut(s)	723, 1006
BspMAI	CTGCAG	2 cut(s)	470, 1333
BspPI	GGATC	3 cut(s)	88, 999, 1012
BspT104I	TTCGAA	2 cut(s)	411, 705
BspTNI	GGTCTC	1 cut(s)	1226
BsrDI	GCAATG	2 cut(s)	723, 931
BsrI	ACTGG	1 cut(s)	847
BssECI	CCNNGG	5 cut(s)	803, 867, 1056, 1252, 1282
BssMI	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
BssSI	CACGAG	1 cut(s)	660
BssT1I	CCWWGG	4 cut(s)	803, 867, 1056, 1252
Bst2BI	CACGAG	1 cut(s)	660
Bst2UI	CCWGG	2 cut(s)	1179, 1283
Bst4CI	ACNGT	1 cut(s)	7
Bst6I	CTCTTC	4 cut(s)	728, 764, 803, 924
BstBAI	YACGTR	2 cut(s)	14, 148
BstBI	TTCGAA	2 cut(s)	411, 705
BstDEI	CTNAG	6 cut(s)	264, 291, 479, 558, 564, 1237
BstDSI	CCRYGG	1 cut(s)	867
BstF5I	GGATG	4 cut(s)	66, 540, 744, 1450
BstFNI	CGCG	2 cut(s)	77, 255
BstHHI	GCGC	2 cut(s)	270, 435
BstKTI	GATC	8 cut(s)	75, 96, 315, 441, 478, 744, 1007, 1440
BstMAI	GTCTC	1 cut(s)	1226
BstMBI	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
BstMCI	CGRYCG	1 cut(s)	75
BstMWI	GCNNNNNNNGC	3 cut(s)	137, 672, 869
BstNI	CCWGG	2 cut(s)	1179, 1283
BstNSI	RCATGY	2 cut(s)	1195, 1207
BstSCI	CCNGG	6 cut(s)	22, 835, 1014, 1177, 1281, 1585
BstSFI	CTRYAG	2 cut(s)	466, 1329
BstUI	CGCG	2 cut(s)	77, 255
BstV1I	GCAGC	4 cut(s)	299, 662, 850, 1363
BstV2I	GAAGAC	2 cut(s)	102, 1588
BstX2I	RGATCY	1 cut(s)	1004
BstYI	RGATCY	1 cut(s)	1004
BsuRI	GGCC	3 cut(s)	249, 1177, 1594
BtgI	CCRYGG	1 cut(s)	867
BtsCI	GGATG	4 cut(s)	66, 540, 744, 1450
BtsI	GCAGTG	2 cut(s)	463, 1161
BtsIMutI	CAGTG	3 cut(s)	12, 463, 1161
BtuMI	TCGCGA	1 cut(s)	77
CfoI	GCGC	2 cut(s)	270, 435
Cfr13I	GGNCC	1 cut(s)	830
CseI	GACGC	1 cut(s)	587
Csp6I	GTAC	3 cut(s)	27, 538, 840
CviAII	CATG	5 cut(s)	436, 868, 1192, 1204, 1596
CviQI	GTAC	3 cut(s)	27, 538, 840
DdeI	CTNAG	6 cut(s)	264, 291, 479, 558, 564, 1237
DpnI	GATC	8 cut(s)	74, 95, 314, 440, 477, 743, 1006, 1439
DpnII	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
EaeI	YGGCCR	2 cut(s)	247, 1592
Eam1104I	CTCTTC	4 cut(s)	728, 764, 803, 924
EarI	CTCTTC	4 cut(s)	728, 764, 803, 924
EciI	GGCGGA	1 cut(s)	112
Eco130I	CCWWGG	4 cut(s)	803, 867, 1056, 1252
Eco31I	GGTCTC	1 cut(s)	1226
Eco47I	GGWCC	1 cut(s)	830
Eco57I	CTGAAG	4 cut(s)	66, 231, 632, 752
Eco72I	CACGTG	1 cut(s)	14
Eco88I	CYCGRG	2 cut(s)	1069, 1557
EcoRII	CCWGG	2 cut(s)	1177, 1281
EcoT14I	CCWWGG	4 cut(s)	803, 867, 1056, 1252
ErhI	CCWWGG	4 cut(s)	803, 867, 1056, 1252
FaeI	CATG	5 cut(s)	439, 871, 1195, 1207, 1599
FatI	CATG	5 cut(s)	435, 867, 1191, 1203, 1595
FauNDI	CATATG	1 cut(s)	222
FbaI	TGATCA	1 cut(s)	741
FblI	GTMKAC	3 cut(s)	681, 953, 1075
Fnu4HI	GCNGC	5 cut(s)	288, 676, 864, 1175, 1377
FokI	GGATG	4 cut(s)	73, 527, 751, 1457
Fsp4HI	GCNGC	5 cut(s)	288, 676, 864, 1175, 1377
FspBI	CTAG	5 cut(s)	203, 329, 686, 912, 1157
GlaI	GCGC	2 cut(s)	269, 434
GluI	GCNGC	5 cut(s)	288, 676, 864, 1175, 1377
GsaI	CCCAGC	1 cut(s)	698
HaeIII	GGCC	3 cut(s)	249, 1177, 1594
HapII	CCGG	6 cut(s)	23, 725, 837, 1015, 1269, 1586
HgaI	GACGC	1 cut(s)	587
HhaI	GCGC	2 cut(s)	270, 435
Hin1II	CATG	5 cut(s)	439, 871, 1195, 1207, 1599
Hin6I	GCGC	2 cut(s)	268, 433
HinP1I	GCGC	2 cut(s)	268, 433
HincII	GTYRAC	1 cut(s)	1134
HindII	GTYRAC	1 cut(s)	1134
HindIII	AAGCTT	1 cut(s)	614
HinfI	GANTC	6 cut(s)	226, 262, 481, 533, 607, 1019
HpaI	GTTAAC	1 cut(s)	1134
HpaII	CCGG	6 cut(s)	23, 725, 837, 1015, 1269, 1586
Hpy166II	GTNNAC	5 cut(s)	682, 833, 954, 1076, 1134
Hpy188I	TCNGA	7 cut(s)	46, 72, 451, 480, 612, 1051, 1529
Hpy188III	TCNNGA	6 cut(s)	76, 392, 1262, 1289, 1382, 1557
Hpy8I	GTNNAC	5 cut(s)	682, 833, 954, 1076, 1134
Hpy99I	CGWCG	1 cut(s)	242
HpyAV	CCTTC	4 cut(s)	456, 1292, 1348, 1379
HpyCH4III	ACNGT	1 cut(s)	7
HpyCH4IV	ACGT	6 cut(s)	13, 147, 240, 630, 637, 1276
HpyCH4V	TGCA	9 cut(s)	85, 287, 468, 996, 1166, 1195, 1215, 1331, 1484
HpyF10VI	GCNNNNNNNGC	3 cut(s)	137, 672, 869
HpyF3I	CTNAG	6 cut(s)	264, 291, 479, 558, 564, 1237
HpySE526I	ACGT	6 cut(s)	13, 147, 240, 630, 637, 1276
Hsp92II	CATG	5 cut(s)	439, 871, 1195, 1207, 1599
HspAI	GCGC	2 cut(s)	268, 433
Ksp22I	TGATCA	1 cut(s)	741
KspAI	GTTAAC	1 cut(s)	1134
Kzo9I	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
LmnI	GCTCC	5 cut(s)	136, 672, 721, 727, 1345
Lsp1109I	GCAGC	4 cut(s)	299, 662, 850, 1363
LweI	GCATC	5 cut(s)	173, 198, 274, 1005, 1363
MaeI	CTAG	5 cut(s)	203, 329, 686, 912, 1157
MaeII	ACGT	6 cut(s)	13, 147, 240, 630, 637, 1276
MaeIII	GTNAC	5 cut(s)	143, 379, 815, 1060, 1277
MalI	GATC	8 cut(s)	74, 95, 314, 440, 477, 743, 1006, 1439
MboI	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
MfeI	CAATTG	2 cut(s)	667, 1479
MflI	RGATCY	1 cut(s)	1004
MhlI	GDGCHC	2 cut(s)	693, 1316
MlsI	TGGCCA	2 cut(s)	249, 1594
MluNI	TGGCCA	2 cut(s)	249, 1594
MlyI	GAGTC	4 cut(s)	220, 256, 490, 527
MmeI	TCCRAC	1 cut(s)	38
Mox20I	TGGCCA	2 cut(s)	249, 1594
MscI	TGGCCA	2 cut(s)	249, 1594
MseI	TTAA	4 cut(s)	420, 573, 888, 1133
MslI	CAYNNNNRTG	1 cut(s)	1202
Msp20I	TGGCCA	2 cut(s)	249, 1594
MspA1I	CMGCKG	1 cut(s)	290
MspI	CCGG	6 cut(s)	23, 725, 837, 1015, 1269, 1586
MspR9I	CCNGG	6 cut(s)	24, 837, 1016, 1179, 1283, 1587
MunI	CAATTG	2 cut(s)	667, 1479
Mva1269I	GAATGC	1 cut(s)	1257
MvaI	CCWGG	2 cut(s)	1179, 1283
MvnI	CGCG	2 cut(s)	77, 255
MwoI	GCNNNNNNNGC	3 cut(s)	137, 672, 869
NciI	CCSGG	4 cut(s)	24, 837, 1016, 1587
NcoI	CCATGG	1 cut(s)	867
NdeI	CATATG	1 cut(s)	222
NdeII	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
NlaIII	CATG	5 cut(s)	439, 871, 1195, 1207, 1599
NlaIV	GGNNCC	2 cut(s)	723, 1006
NmuCI	GTSAC	1 cut(s)	1277
NruI	TCGCGA	1 cut(s)	77
NspI	RCATGY	2 cut(s)	1195, 1207
NspV	TTCGAA	2 cut(s)	411, 705
PaeR7I	CTCGAG	1 cut(s)	1557
PctI	GAATGC	1 cut(s)	1257
PfeI	GAWTC	2 cut(s)	607, 1019
PkrI	GCNGC	5 cut(s)	289, 677, 865, 1176, 1378
Ple19I	CGATCG	1 cut(s)	75
PleI	GAGTC	4 cut(s)	220, 256, 489, 527
PmaCI	CACGTG	1 cut(s)	14
PmlI	CACGTG	1 cut(s)	14
PpsI	GAGTC	4 cut(s)	220, 256, 489, 527
Ppu21I	YACGTR	2 cut(s)	14, 148
Psp1406I	AACGTT	1 cut(s)	637
Psp6I	CCWGG	2 cut(s)	1177, 1281
PspCI	CACGTG	1 cut(s)	14
PspFI	CCCAGC	1 cut(s)	694
PspGI	CCWGG	2 cut(s)	1177, 1281
PspN4I	GGNNCC	2 cut(s)	723, 1006
PspPI	GGNCC	1 cut(s)	830
PstI	CTGCAG	2 cut(s)	470, 1333
PsuI	RGATCY	1 cut(s)	1004
PvuI	CGATCG	1 cut(s)	75
PvuII	CAGCTG	1 cut(s)	290
RruI	TCGCGA	1 cut(s)	77
RsaI	GTAC	3 cut(s)	28, 539, 841
RsaNI	GTAC	3 cut(s)	27, 538, 840
RseI	CAYNNNNRTG	1 cut(s)	1202
SaqAI	TTAA	4 cut(s)	420, 573, 888, 1133
SatI	GCNGC	5 cut(s)	288, 676, 864, 1175, 1377
Sau3AI	GATC	8 cut(s)	72, 93, 312, 438, 475, 741, 1004, 1437
Sau96I	GGNCC	1 cut(s)	830
SchI	GAGTC	4 cut(s)	220, 256, 490, 527
ScrFI	CCNGG	6 cut(s)	24, 837, 1016, 1179, 1283, 1587
SduI	GDGCHC	2 cut(s)	693, 1316
SfaNI	GCATC	5 cut(s)	173, 198, 274, 1005, 1363
SfcI	CTRYAG	2 cut(s)	466, 1329
Sfr274I	CTCGAG	1 cut(s)	1557
SfuI	TTCGAA	2 cut(s)	411, 705
SinI	GGWCC	1 cut(s)	830
SlaI	CTCGAG	1 cut(s)	1557
SmiMI	CAYNNNNRTG	1 cut(s)	1202
SmlI	CTYRAG	2 cut(s)	390, 1557
SmoI	CTYRAG	2 cut(s)	390, 1557
SpeI	ACTAGT	1 cut(s)	911
SsiI	CCGC	4 cut(s)	123, 255, 1110, 1174
SspMI	CTAG	5 cut(s)	203, 329, 686, 912, 1157
StyD4I	CCNGG	6 cut(s)	22, 835, 1014, 1177, 1281, 1585
StyI	CCWWGG	4 cut(s)	803, 867, 1056, 1252
TaaI	ACNGT	1 cut(s)	7
TaiI	ACGT	6 cut(s)	16, 150, 243, 633, 640, 1279
TaqI	TCGA	9 cut(s)	237, 411, 705, 999, 1089, 1183, 1244, 1440, 1558
TauI	GCSGC	1 cut(s)	1177
TfiI	GAWTC	2 cut(s)	607, 1019
Tru1I	TTAA	4 cut(s)	420, 573, 888, 1133
Tru9I	TTAA	4 cut(s)	420, 573, 888, 1133
TscAI	CASTG	3 cut(s)	12, 470, 1168
TseFI	GTSAC	1 cut(s)	1277
TseI	GCWGC	4 cut(s)	287, 675, 863, 1376
Tsp45I	GTSAC	1 cut(s)	1277
TspDTI	ATGAA	5 cut(s)	605, 782, 942, 1367, 1461
TspRI	CASTG	3 cut(s)	12, 470, 1168
VpaK11BI	GGWCC	1 cut(s)	830
XapI	RAATTY	4 cut(s)	79, 1467, 1520, 1551
XceI	RCATGY	2 cut(s)	1195, 1207
XcmI	CCANNNNNNNNNTGG	1 cut(s)	659
XhoI	CTCGAG	1 cut(s)	1557
XmiI	GTMKAC	3 cut(s)	681, 953, 1075
XspI	CTAG	5 cut(s)	203, 329, 686, 912, 1157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.