Rmu_sc0004158.1_g000001

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004158.1
OG0003681
Physical Location & Seq
Reverse (-)
2 .. 1058
1057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004158.1_g000001.1.cds

Sequence Viewer

Length: 1057 bp
atggaggagcagcaatattatgatgaccacagcaattccagtttcaaagatataatgcccacagctatatcctcctttttctcggcctatgccccctttgctggttacatgtttatgctccaatccatgagcaccaatttcctcccttacgagtttaactcatacgtatactcgtacttaaaatcaagcctcagctacctcttggaccctctagccaatcacataactctcctcatcgacaagtctagcgatgaagactacttggacgaaatctatgattctgctgaggtctatcttgccaccaagatcaacccgaaaacccaaatctttcgaattagaaaagcaccgaaacaaaaggtccccatccttgatgttaccaccaaccaagaaatcactgacactttcgaagacattaacgtgaagtggcggatgatcttccctacagaaaacgatggttactacagtagcgtctataagagccgcatggagctaacattccacaggaaacacaaagacaaagttataaactcgtacatcccctatgtgttggctcgggccaaggctatcaaagatgcagaaaagactctgaagctttatagttacaatagtaaacgttcctcggtcttcaaaatttctcgtggacgctctcatggttctgatgacgatgatgactttgcaccttcttcgggcaaatggagttgcatcgatcttgagcacccggctacttttgagacaatggcaatggagccggagctgaagaggatgatcatagaggatttggacaagtttgtgaagaggagggagttctacaagagagttggtaaggcttggaagagaggttacttgttgtatggtcctcccggtactggaaagtctagcttggttgcggccatggctaatcatctccattttaatatttatgacttggaacttactagtatttccagcaatgataagttgaggaaggccttgatgtctactacaaatcgttctattgtggtgtttgaggatattgattgcagcaagggatccgtaaaccgggaatcagcagagaaga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

352

Amino Acids

40.71

Weight (kDa)

7.09

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AccI GTMKAC 2 cut(s) 168, 977
AciI CCGC 3 cut(s) 427, 481, 887
AclI AACGTT 1 cut(s) 613
AclWI GGATC 2 cut(s) 1023, 1036
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 1 cut(s) 630
AcuI CTGAAG 2 cut(s) 608, 776
AfaI GTAC 3 cut(s) 176, 533, 865
AfiI CCNNNNNNNGG 4 cut(s) 101, 686, 866, 1038
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 2 cut(s) 46, 628
AhlI ACTAGT 1 cut(s) 935
AluBI AGCT 6 cut(s) 65, 195, 490, 592, 754, 879
AluI AGCT 6 cut(s) 65, 195, 490, 592, 754, 879
Alw21I GWGCWC 2 cut(s) 134, 717
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 2 cut(s) 1023, 1036
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 4 cut(s) 84, 555, 888, 966
ApeKI GCWGC 2 cut(s) 10, 1020
ApoI RAATTY 1 cut(s) 630
AspS9I GGNCC 4 cut(s) 205, 358, 555, 854
AsuC2I CCSGG 3 cut(s) 719, 861, 1040
AsuII TTCGAA 2 cut(s) 331, 405
AvaI CYCGRG 1 cut(s) 552
AvaII GGWCC 3 cut(s) 205, 358, 854
BamHI GGATCC 1 cut(s) 1028
BarI GAAGNNNNNNTAC 2 cut(s) 824, 856
BauI CACGAG 1 cut(s) 636
BbsI GAAGAC 3 cut(s) 261, 414, 616
Bbv12I GWGCWC 2 cut(s) 134, 717
BbvCI CCTCAGC 2 cut(s) 191, 285
BbvI GCAGC 2 cut(s) 22, 1032
BccI CCATC 2 cut(s) 371, 446
BcgI CGANNNNNNTGC 2 cut(s) 666, 700
BclI TGATCA 1 cut(s) 765
BcnI CCSGG 3 cut(s) 719, 861, 1040
BcoDI GTCTC 1 cut(s) 725
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 4 cut(s) 212, 246, 876, 936
BfmI CTRYAG 2 cut(s) 441, 460
BisI GCNGC 4 cut(s) 11, 481, 888, 1021
BlsI GCNGC 4 cut(s) 12, 482, 889, 1022
Bme1390I CCNGG 3 cut(s) 719, 861, 1040
Bme18I GGWCC 3 cut(s) 205, 358, 854
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 4 cut(s) 205, 358, 555, 854
BmiI GGNNCC 4 cut(s) 207, 360, 747, 1030
BmrFI CCNGG 3 cut(s) 719, 861, 1040
BmsI GCATC 2 cut(s) 562, 711
BpiI GAAGAC 3 cut(s) 261, 414, 616
BplI GAGNNNNNCTC 2 cut(s) 143, 175
Bpu10I CCTNAGC 2 cut(s) 191, 285
Bpu14I TTCGAA 2 cut(s) 331, 405
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 3 cut(s) 719, 861, 1040
Bsa29I ATCGAT 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 558, 618, 891
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 686, 866, 1038
Bse1I ACTGG 2 cut(s) 39, 871
Bse3DI GCAATG 2 cut(s) 747, 955
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 3 cut(s) 558, 618, 891
BseGI GGATG 4 cut(s) 363, 435, 534, 768
BseLI CCNNNNNNNGG 4 cut(s) 101, 686, 866, 1038
BseMI GCAATG 2 cut(s) 747, 955
BseMII CTCAG 2 cut(s) 205, 276
BseNI ACTGG 2 cut(s) 39, 871
BseRI GAGGAG 3 cut(s) 20, 221, 811
BseXI GCAGC 2 cut(s) 22, 1032
BshFI GGCC 4 cut(s) 86, 557, 890, 968
BshVI ATCGAT 1 cut(s) 705
BsiHKAI GWGCWC 2 cut(s) 134, 717
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 4 cut(s) 719, 749, 861, 1039
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 4 cut(s) 101, 686, 866, 1038
BsmAI GTCTC 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 4 cut(s) 86, 557, 890, 968
BsoBI CYCGRG 1 cut(s) 552
Bsp119I TTCGAA 2 cut(s) 331, 405
Bsp1286I GDGCHC 2 cut(s) 134, 717
Bsp143I GATC 5 cut(s) 306, 432, 706, 765, 1028
Bsp19I CCATGG 1 cut(s) 891
BspACI CCGC 3 cut(s) 427, 481, 887
BspANI GGCC 4 cut(s) 86, 557, 890, 968
BspCNI CTCAG 2 cut(s) 204, 277
BspDI ATCGAT 1 cut(s) 705
BspLI GGNNCC 4 cut(s) 207, 360, 747, 1030
BspPI GGATC 2 cut(s) 1023, 1036
BspT104I TTCGAA 2 cut(s) 331, 405
BsrDI GCAATG 2 cut(s) 747, 955
BsrI ACTGG 2 cut(s) 39, 871
BssECI CCNNGG 3 cut(s) 558, 618, 891
BssMI GATC 5 cut(s) 306, 432, 706, 765, 1028
BssNAI GTATAC 1 cut(s) 169
BssSI CACGAG 1 cut(s) 636
BssT1I CCWWGG 2 cut(s) 558, 891
Bst1107I GTATAC 1 cut(s) 169
Bst2BI CACGAG 1 cut(s) 636
Bst4CI ACNGT 1 cut(s) 464
Bst6I CTCTTC 3 cut(s) 752, 788, 827
BstBAI YACGTR 1 cut(s) 166
BstBI TTCGAA 2 cut(s) 331, 405
BstDEI CTNAG 2 cut(s) 191, 285
BstDSI CCRYGG 1 cut(s) 891
BstF5I GGATG 4 cut(s) 363, 435, 534, 768
BstKTI GATC 5 cut(s) 309, 435, 709, 768, 1031
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 5 cut(s) 306, 432, 706, 765, 1028
BstMWI GCNNNNNNNGC 2 cut(s) 98, 893
BstNSI RCATGY 1 cut(s) 112
BstSCI CCNGG 3 cut(s) 717, 859, 1038
BstSFI CTRYAG 2 cut(s) 441, 460
BstSNI TACGTA 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 22, 1032
BstV2I GAAGAC 3 cut(s) 261, 414, 616
BstX2I RGATCY 1 cut(s) 1028
BstYI RGATCY 1 cut(s) 1028
BstZ17I GTATAC 1 cut(s) 169
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 4 cut(s) 86, 557, 890, 968
BsuTUI ATCGAT 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 891
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 4 cut(s) 363, 435, 534, 768
BtsIMutI CAGTG 1 cut(s) 393
Cfr13I GGNCC 4 cut(s) 205, 358, 555, 854
ClaI ATCGAT 1 cut(s) 705
CseI GACGC 2 cut(s) 457, 651
Csp6I GTAC 3 cut(s) 175, 532, 864
CviAII CATG 5 cut(s) 109, 127, 484, 650, 892
CviQI GTAC 3 cut(s) 175, 532, 864
DdeI CTNAG 2 cut(s) 191, 285
DpnI GATC 5 cut(s) 308, 434, 708, 767, 1030
DpnII GATC 5 cut(s) 306, 432, 706, 765, 1028
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 3 cut(s) 752, 788, 827
EarI CTCTTC 3 cut(s) 752, 788, 827
EciI GGCGGA 1 cut(s) 442
Eco105I TACGTA 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 558, 891
Eco147I AGGCCT 1 cut(s) 968
Eco47I GGWCC 3 cut(s) 205, 358, 854
Eco57I CTGAAG 2 cut(s) 608, 776
Eco88I CYCGRG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 358
EcoT14I CCWWGG 2 cut(s) 558, 891
ErhI CCWWGG 2 cut(s) 558, 891
FaeI CATG 5 cut(s) 112, 130, 487, 653, 895
FalI AAGNNNNNCTT 2 cut(s) 863, 895
FaqI GGGAC 1 cut(s) 344
FatI CATG 5 cut(s) 108, 126, 483, 649, 891
FbaI TGATCA 1 cut(s) 765
FblI GTMKAC 2 cut(s) 168, 977
Fnu4HI GCNGC 4 cut(s) 11, 481, 888, 1021
FokI GGATG 4 cut(s) 350, 442, 521, 775
Fsp4HI GCNGC 4 cut(s) 11, 481, 888, 1021
FspBI CTAG 4 cut(s) 212, 246, 876, 936
GluI GCNGC 4 cut(s) 11, 481, 888, 1021
HaeIII GGCC 4 cut(s) 86, 557, 890, 968
HapII CCGG 4 cut(s) 719, 749, 861, 1039
HgaI GACGC 2 cut(s) 457, 651
Hin1II CATG 5 cut(s) 112, 130, 487, 653, 895
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 278, 583, 1043
HpaII CCGG 4 cut(s) 719, 749, 861, 1039
Hpy166II GTNNAC 5 cut(s) 169, 611, 641, 978, 1036
Hpy188I TCNGA 2 cut(s) 588, 658
Hpy188III TCNNGA 1 cut(s) 710
Hpy8I GTNNAC 5 cut(s) 169, 611, 641, 978, 1036
HpyAV CCTTC 2 cut(s) 690, 958
HpyCH4III ACNGT 1 cut(s) 464
HpyCH4IV ACGT 3 cut(s) 165, 417, 613
HpyCH4V TGCA 4 cut(s) 575, 677, 702, 1020
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 893
HpyF3I CTNAG 2 cut(s) 191, 285
HpySE526I ACGT 3 cut(s) 165, 417, 613
Hsp92II CATG 5 cut(s) 112, 130, 487, 653, 895
Ksp22I TGATCA 1 cut(s) 765
Kzo9I GATC 5 cut(s) 306, 432, 706, 765, 1028
LmnI GCTCC 5 cut(s) 7, 123, 487, 745, 751
LpnPI CCDG 9 cut(s) 52, 87, 487, 732, 762, 852, 874, 958, 1052
Lsp1109I GCAGC 2 cut(s) 22, 1032
LweI GCATC 2 cut(s) 562, 711
MaeI CTAG 4 cut(s) 212, 246, 876, 936
MaeII ACGT 3 cut(s) 165, 417, 613
MaeIII GTNAC 5 cut(s) 104, 373, 455, 599, 839
MalI GATC 5 cut(s) 308, 434, 708, 767, 1030
MboI GATC 5 cut(s) 306, 432, 706, 765, 1028
MboII GAAGA 8 cut(s) 266, 419, 427, 616, 675, 769, 805, 844
MflI RGATCY 1 cut(s) 1028
MhlI GDGCHC 2 cut(s) 134, 717
MluCI AATT 4 cut(s) 34, 136, 333, 630
MlyI GAGTC 1 cut(s) 577
MseI TTAA 4 cut(s) 156, 179, 414, 912
MslI CAYNNNNRTG 2 cut(s) 113, 416
MspI CCGG 4 cut(s) 719, 749, 861, 1039
MspR9I CCNGG 3 cut(s) 719, 861, 1040
MwoI GCNNNNNNNGC 2 cut(s) 98, 893
NciI CCSGG 3 cut(s) 719, 861, 1040
NcoI CCATGG 1 cut(s) 891
NdeII GATC 5 cut(s) 306, 432, 706, 765, 1028
NlaIII CATG 5 cut(s) 112, 130, 487, 653, 895
NlaIV GGNNCC 4 cut(s) 207, 360, 747, 1030
NmeAIII GCCGAG 1 cut(s) 62
NspI RCATGY 1 cut(s) 112
NspV TTCGAA 2 cut(s) 331, 405
PceI AGGCCT 1 cut(s) 968
PciI ACATGT 1 cut(s) 108
PfeI GAWTC 2 cut(s) 278, 1043
PkrI GCNGC 4 cut(s) 12, 482, 889, 1022
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
Ppu21I YACGTR 1 cut(s) 166
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 108
PsiI TTATAA 1 cut(s) 524
Psp1406I AACGTT 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 358
PspN4I GGNNCC 4 cut(s) 207, 360, 747, 1030
PspPI GGNCC 4 cut(s) 205, 358, 555, 854
PspPPI RGGWCCY 1 cut(s) 358
PsuI RGATCY 1 cut(s) 1028
RsaI GTAC 3 cut(s) 176, 533, 865
RsaNI GTAC 3 cut(s) 175, 532, 864
RseI CAYNNNNRTG 2 cut(s) 113, 416
SaqAI TTAA 4 cut(s) 156, 179, 414, 912
SatI GCNGC 4 cut(s) 11, 481, 888, 1021
Sau3AI GATC 5 cut(s) 306, 432, 706, 765, 1028
Sau96I GGNCC 4 cut(s) 205, 358, 555, 854
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 3 cut(s) 719, 861, 1040
SduI GDGCHC 2 cut(s) 134, 717
SfaNI GCATC 2 cut(s) 562, 711
SfcI CTRYAG 2 cut(s) 441, 460
SfuI TTCGAA 2 cut(s) 331, 405
SinI GGWCC 3 cut(s) 205, 358, 854
SmiMI CAYNNNNRTG 2 cut(s) 113, 416
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SnaBI TACGTA 1 cut(s) 166
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 4 cut(s) 34, 136, 333, 630
SseBI AGGCCT 1 cut(s) 968
SsiI CCGC 3 cut(s) 427, 481, 887
SspI AATATT 2 cut(s) 17, 916
SspMI CTAG 4 cut(s) 212, 246, 876, 936
StuI AGGCCT 1 cut(s) 968
StyD4I CCNGG 3 cut(s) 717, 859, 1038
StyI CCWWGG 2 cut(s) 558, 891
TaaI ACNGT 1 cut(s) 464
TaiI ACGT 3 cut(s) 168, 420, 616
TaqI TCGA 4 cut(s) 237, 331, 405, 705
TaqII GACCGA 1 cut(s) 610
TasI AATT 4 cut(s) 34, 136, 333, 630
TauI GCSGC 2 cut(s) 483, 890
TfiI GAWTC 2 cut(s) 278, 1043
Tru1I TTAA 4 cut(s) 156, 179, 414, 912
Tru9I TTAA 4 cut(s) 156, 179, 414, 912
TscAI CASTG 1 cut(s) 400
TseI GCWGC 2 cut(s) 10, 1020
TspDTI ATGAA 1 cut(s) 267
TspGWI ACGGA 1 cut(s) 1021
TspRI CASTG 1 cut(s) 400
VpaK11BI GGWCC 3 cut(s) 205, 358, 854
XapI RAATTY 1 cut(s) 630
XceI RCATGY 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 168, 977
XspI CTAG 4 cut(s) 212, 246, 876, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.