Rw2G010280

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003681
Physical Location & Seq
Reverse (-)
10384004 .. 10388845
4842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010280.1

Sequence Viewer

Length: 3138 bp
ATGGAGGAGCAGCAATATTATGATGACCACAGCAATTCCAATTTCAAAGATATAATGCCCACAGCTATATCCTCCTTTTTCTCGGCCTATGCCCCCTTTGCTGGTTACATGTTTATGCTCCAATCCATGAACACCAATTTCCTCCCTTACGAGTTTAACTCATACGTATACTCGTACTTAAAATCAAGCCTCAGCTACCTCTTGGACCCTCTAGCCAATCACATAACTCTCCTCATCGACAACTCTAGCGATGAAGACTACTCGGACGAAATCTATGATTCTGCTGAGGTCTATCTTGCCACCAAGATCAACCCGAAAACCCAAATCTTTCGAATTAGAAAAGCACCGAAACAAAAGGTCCCCATCCTTGATGTTACCACCAACCAAGAAATCACTGACACTTTCGAAGACATTAACGTGAAGTGGCGGATGATCTTCCCTACAGAAAACGATGGTTACTACAGTAGCGTCTATAAGAGCCGCATGGAGCTAACATTCCACAGGAAACACAAGGACAAAGTTATAAACTCGTACATCCCCTATGTGTTGGCTCGGGCCAAGGCTATCAAAGATGCAGAAAAGACTCTGAAGCTTTATAGTTACAATAGTAAACGTTCCTCGGTCTTCAAAATTTCTCGTGGACGCTCTCATGGTTCTGATGACGATGATGACTTTGCACCTTCTTCGGGCAAATGGAGTTGCATCGATCTTGAGCACCCGGCTACTTTTGAGACAATGGCAATGGAGCCGGAGCTGAAGAGGATGATCATAGAGGATTTGGACAAGTTTGTGAAGAGGAGGGAGTTCTACAAGAGAGTTGGTAAGGCTTGGAAGAGAGGTTACTTGTTGTATGGTCCTCCCGGTACTGGAAAGTCTAGCTTGGTTGCGGCCATGGCTAATCATCTCCATTTTAATATTTATGACTTGGAACTTACTAGTATTTCCAGCAATGATAAGTTGAGGAAGGCCTTGATGTCCACTACAAATCGTTCTATTGTGGTGTTTGAGGATATTGATTGCAGCAAGGGATCCGTAAACCGGGAATCAGCAGAGAAGAAGTTTATGGTTTCCGAAGCCAAGTTTACACTCTCGGGTCTACTGAATGTCGTCGATGGTCTGTGGTCAAGTTGTGGTGATGAGAGGATTATTGTGTTTACCACCAACCACAAGGAGCGGCTAGACCCTGCATTGTTGAGGCCTGGTCGAATGGACATGCACATTCACATGTCGTATTGCACCCCAAAAGGGTTCAAGACCTTGGCTTCAAACTACCTCGGCATTCAGGACACCAGCGAACATCGCCTGTGTGGAGAAATTGAAGCATTGGTGGAGAGCACTAACATCACCCCTGCAGAGGTTTGTGAGGAGCTTATGAGGGGTGGGGGTGATGGTGATGAAGCCGATGCTGCTCTTGAAGGACTTGCCAATTGTCTCAAGCGTAAGAGGCTCGAGACCAACAAGATAGAGGATGAAGAGGTTGGGAATGCAAAGAGGCAGAAAACAGATTCTAATGCATCAGAAATTTCCGAAAATTGTTATTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAACTTCCATAGTTTTTATTGTTTGTCATCAACTCATTATAAAATACCCCATCATATCCTTCAACTCCCAACTCAAAATTTCTCCAAACACTCAAGTCAATAACACAATGTTTACTCTCAATCCCAAAGATATGCAAACCACAGCATCCACATTGTTCTCAGCCTATGCCTCCTTTGCTGCATCAATGATGTTGGTCCGCTCCATTACAGACCAACTCATTCCCCACCAGTTTCGCTCATACATATACTCAGTCTTCAGTCGCTTTTTTACCACTCCCTCCTCGAACCTCACTTTGATTGTTGAAGAGTGCTGTGGTATGACACGAAACCAAGTCTATGATACTGCGGAAGTTTACCTCCAAACAAAGATCAATCCTTTCGCTGAACGCCTCAAGATCAGCAAAACACCCCGGAAAAAGAACATTAGCATTGCCATGGACAAAGGCGAAGAAATCACCGACACCTTTGACAATGATATCAAATTGAAGTGGCGCTTCACCGAGCCTGAGAAAAAACAGAAGGACGAGTACCACTCTGCTAATGACAGTAAGCGTCGGTTTGAGCTAGTGTTTGACAAGAAACACAAGGACACGGTGATGGACTCTTACTTGCCCTACGTTTTTGCTCGAGCTAAAGCCATTCAAGAAGAGGAAAAGGTTGTTAGGCTTTACACCCGGGGTTCGAGTCTGGGTGATGATGAGAACGATAACAGGGGTTCAAGTGGTTGGGGTTCCATAAGGATGGAGCATCCATCTAATTTTGGAACAATGGCGATGGATCCAGAGCTTAAAAGAATAGTTATTGATGATTTGGATAGGTTTGTGAGGAGGAGGGAGTTTTACAAAAGGGTTGGTAAGGCTTGGAAGAGAGGGTATTTGTTGTATGGTCCACCTGGTACAGGTAAATCGAGCTTGATTGCAGCCATGGCTAACTATCTCAAGTTTGATGTTTATGATTTGGAGCTTTCTGGCGTTCACAGTAATAGTGATTTGAGGAGGGTATTGCTTTCTACTAAAAATCGTTCTATTATGGTGATTGAGGATATTGATTGCTGCGCCAAAGTGCAGAACAGGGGACGTGAACCGGATCCACGTTCTCATAACAACAGGTTGACACTATCAGGCCTACTGAACTTCATAGATGGTCTGTGGTCAAGCTGCGGGGACGAGAGAATTATAGTTTTCACCACCAACCACAAGGATCGGTTAGACCCTGCACTGCTGCGTCCGGGTCGCATGGATGTGCACATTCACATGTCTTATTGCACTGCAAAAGGATTCGACATCTTAGCCTCCAACTACCTAGGCATTCATGAGAGCAACCCTCATCAGCTCTGCAAAGAAGTTAAAGGGTTGATAGAGAGCACTGAGGTCACCCCTGCAGAGGTTGCTGAGGAGCTCATGAAAAGTGATGATGCTAATGGTACTCTCAAAGGGCTTGTCAATTTCCTCAAGAAAAAGAAGGTCGAGAATGATAAAGTGAAGGTAGGAGGGGCTGACAAAACTGAAGATCAAGAGCCATTTCCGGATGACTATTTACCACTCTATGATGGCTTTGACTTATTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

1045

Amino Acids

119.47

Weight (kDa)

7.1

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 49 - 144 1.1e-13 Domain associated at C-terminal with AAA
AAA PF00004 281 - 410 1.4e-19 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 412 - 485 6.8e-19 At3g28540-like, AAA+ ATPase lid domain
AAA_assoc PF14363 596 - 689 8.8e-24 Domain associated at C-terminal with AAA
AAA PF00004 814 - 942 5.6e-21 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 944 - 1014 2.7e-20 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 524, 1607
AccBSI CCGCTC 2 cut(s) 1174, 1767
AccI GTMKAC 2 cut(s) 168, 1096
AccIII TCCGGA 1 cut(s) 3091
AciI CCGC 7 cut(s) 427, 481, 887, 1174, 1765, 1913, 2727
AclI AACGTT 1 cut(s) 613
AclWI GGATC 7 cut(s) 1023, 1036, 2339, 2352, 2648, 2661, 2775
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 3 cut(s) 630, 1521, 1644
AcuI CTGAAG 4 cut(s) 608, 776, 1807, 3093
AfaI GTAC 6 cut(s) 176, 533, 865, 2096, 2464, 2992
AfiI CCNNNNNNNGG 8 cut(s) 101, 686, 866, 1038, 1245, 1354, 2465, 2950
AflIII ACRYGT 3 cut(s) 108, 1224, 2820
AhlI ACTAGT 1 cut(s) 935
AjiI CACGTC 1 cut(s) 2645
AjnI CCWGG 2 cut(s) 1198, 2458
Alw21I GWGCWC 5 cut(s) 717, 1337, 2814, 2933, 2967
Alw26I GTCTC 3 cut(s) 725, 1436, 1445
Alw44I GTGCAC 1 cut(s) 2810
AlwI GGATC 7 cut(s) 1023, 1036, 2339, 2352, 2648, 2661, 2775
Ama87I CYCGRG 5 cut(s) 552, 1090, 1448, 2193, 2241
Aor13HI TCCGGA 1 cut(s) 3091
AoxI GGCC 6 cut(s) 84, 555, 888, 966, 1196, 2689
ApaLI GTGCAC 1 cut(s) 2810
ApeKI GCWGC 8 cut(s) 10, 1020, 1406, 1745, 2486, 2619, 2724, 2788
ApoI RAATTY 3 cut(s) 630, 1521, 1644
ArsI GACNNNNNNTTYG 2 cut(s) 2991, 3023
AspA2I CCTAGG 1 cut(s) 2869
AspLEI GCGC 2 cut(s) 2061, 2624
AspS9I GGNCC 6 cut(s) 205, 358, 555, 854, 1762, 2453
AsuC2I CCSGG 7 cut(s) 719, 861, 1040, 1978, 2242, 2243, 2796
AsuII TTCGAA 2 cut(s) 331, 405
AvaI CYCGRG 5 cut(s) 552, 1090, 1448, 2193, 2241
AvaII GGWCC 5 cut(s) 205, 358, 854, 1762, 2453
AvrII CCTAGG 1 cut(s) 2869
BaeGI GKGCMC 1 cut(s) 2814
BamHI GGATCC 3 cut(s) 1028, 2344, 2653
BanII GRGCYC 1 cut(s) 2967
BarI GAAGNNNNNNTAC 4 cut(s) 824, 856, 2078, 2110
BauI CACGAG 1 cut(s) 636
BbsI GAAGAC 4 cut(s) 261, 414, 616, 1813
Bbv12I GWGCWC 5 cut(s) 717, 1337, 2814, 2933, 2967
BbvCI CCTCAGC 3 cut(s) 191, 285, 2958
BbvI GCAGC 8 cut(s) 22, 1032, 1393, 1732, 2498, 2606, 2711, 2775
BcgI CGANNNNNNTGC 2 cut(s) 666, 700
BciT130I CCWGG 2 cut(s) 1200, 2460
BclI TGATCA 1 cut(s) 765
BcnI CCSGG 7 cut(s) 719, 861, 1040, 1978, 2242, 2243, 2796
BcoDI GTCTC 3 cut(s) 725, 1436, 1445
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 7 cut(s) 212, 246, 876, 936, 1178, 2132, 2870
BfmI CTRYAG 4 cut(s) 441, 460, 1350, 2946
BfoI RGCGCY 1 cut(s) 2062
BlnI CCTAGG 1 cut(s) 2869
Bme1390I CCNGG 9 cut(s) 719, 861, 1040, 1200, 1978, 2242, 2243, 2460, 2796
Bme18I GGWCC 5 cut(s) 205, 358, 854, 1762, 2453
BmeT110I CYCGRG 5 cut(s) 552, 1090, 1448, 2193, 2241
BmgBI CACGTC 1 cut(s) 2645
BmgT120I GGNCC 6 cut(s) 205, 358, 555, 854, 1762, 2453
BmiI GGNNCC 7 cut(s) 207, 360, 747, 1030, 2299, 2346, 2655
BmrFI CCNGG 9 cut(s) 719, 861, 1040, 1200, 1978, 2242, 2243, 2460, 2796
BmsI GCATC 8 cut(s) 562, 711, 1393, 1523, 1721, 1757, 2323, 2971
BpiI GAAGAC 4 cut(s) 261, 414, 616, 1813
BplI GAGNNNNNCTC 6 cut(s) 143, 175, 2084, 2116, 2875, 2907
Bpu10I CCTNAGC 3 cut(s) 191, 285, 2958
Bpu14I TTCGAA 2 cut(s) 331, 405
BpuEI CTTGAG 6 cut(s) 731, 1418, 1644, 1943, 2489, 3002
BpuMI CCSGG 7 cut(s) 719, 861, 1040, 1978, 2242, 2243, 2796
Bsa29I ATCGAT 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 1445
BsaWI WCCGGW 2 cut(s) 2650, 3091
Bsc4I CCNNNNNNNGG 8 cut(s) 101, 686, 866, 1038, 1245, 1354, 2465, 2950
Bse1I ACTGG 2 cut(s) 871, 1795
Bse3DI GCAATG 3 cut(s) 747, 955, 1995
BseAI TCCGGA 1 cut(s) 3091
BseBI CCWGG 2 cut(s) 1200, 2460
BseCI ATCGAT 1 cut(s) 705
BseLI CCNNNNNNNGG 8 cut(s) 101, 686, 866, 1038, 1245, 1354, 2465, 2950
BseMI GCAATG 3 cut(s) 747, 955, 1995
BseMII CTCAG 7 cut(s) 205, 276, 1740, 1830, 2064, 2925, 2949
BseNI ACTGG 2 cut(s) 871, 1795
BseRI GAGGAG 9 cut(s) 20, 221, 811, 1379, 1837, 2407, 2410, 2575, 2975
BseSI GKGCMC 1 cut(s) 2814
BseXI GCAGC 8 cut(s) 22, 1032, 1393, 1732, 2498, 2606, 2711, 2775
BsgI GTGCAG 2 cut(s) 2651, 2766
BshFI GGCC 6 cut(s) 86, 557, 890, 968, 1198, 2691
BshVI ATCGAT 1 cut(s) 705
BsiHKAI GWGCWC 5 cut(s) 717, 1337, 2814, 2933, 2967
BsiHKCI CYCGRG 5 cut(s) 552, 1090, 1448, 2193, 2241
BsiSI CCGG 9 cut(s) 719, 749, 861, 1039, 1978, 2242, 2651, 2795, 3092
BslFI GGGAC 3 cut(s) 344, 2655, 2744
BslI CCNNNNNNNGG 8 cut(s) 101, 686, 866, 1038, 1245, 1354, 2465, 2950
BsmAI GTCTC 3 cut(s) 725, 1436, 1445
BsmFI GGGAC 3 cut(s) 344, 2655, 2744
BsmI GAATGC 3 cut(s) 1278, 1489, 2874
BsnI GGCC 6 cut(s) 86, 557, 890, 968, 1198, 2691
Bso31I GGTCTC 1 cut(s) 1445
BsoBI CYCGRG 5 cut(s) 552, 1090, 1448, 2193, 2241
Bsp119I TTCGAA 2 cut(s) 331, 405
Bsp1286I GDGCHC 5 cut(s) 717, 1337, 2814, 2933, 2967
Bsp13I TCCGGA 1 cut(s) 3091
Bsp19I CCATGG 3 cut(s) 891, 2001, 2490
BspACI CCGC 7 cut(s) 427, 481, 887, 1174, 1765, 1913, 2727
BspANI GGCC 6 cut(s) 86, 557, 890, 968, 1198, 2691
BspCNI CTCAG 7 cut(s) 204, 277, 1739, 1829, 2065, 2926, 2950
BspDI ATCGAT 1 cut(s) 705
BspEI TCCGGA 1 cut(s) 3091
BspHI TCATGA 2 cut(s) 2878, 2967
BspLI GGNNCC 7 cut(s) 207, 360, 747, 1030, 2299, 2346, 2655
BspMAI CTGCAG 2 cut(s) 1354, 2950
BspPI GGATC 7 cut(s) 1023, 1036, 2339, 2352, 2648, 2661, 2775
BspT104I TTCGAA 2 cut(s) 331, 405
BspTNI GGTCTC 1 cut(s) 1445
BsrBI CCGCTC 2 cut(s) 1174, 1767
BsrDI GCAATG 3 cut(s) 747, 955, 1995
BsrI ACTGG 2 cut(s) 871, 1795
BssNAI GTATAC 1 cut(s) 169
BssSI CACGAG 1 cut(s) 636
BssT1I CCWWGG 6 cut(s) 558, 891, 1257, 2001, 2490, 2869
Bst1107I GTATAC 1 cut(s) 169
Bst2BI CACGAG 1 cut(s) 636
Bst2UI CCWGG 2 cut(s) 1200, 2460
Bst4CI ACNGT 4 cut(s) 464, 2114, 2161, 2546
Bst6I CTCTTC 7 cut(s) 752, 788, 827, 1467, 1866, 2208, 2426
BstAPI GCANNNNNTGC 1 cut(s) 2954
BstBAI YACGTR 1 cut(s) 166
BstBI TTCGAA 2 cut(s) 331, 405
BstDEI CTNAG 8 cut(s) 191, 285, 1726, 1816, 2073, 2854, 2934, 2958
BstDSI CCRYGG 3 cut(s) 891, 2001, 2490
BstEII GGTNACC 1 cut(s) 2938
BstENI CCTNNNNNAGG 1 cut(s) 2463
BstH2I RGCGCY 1 cut(s) 2062
BstHHI GCGC 2 cut(s) 2061, 2624
BstMAI GTCTC 3 cut(s) 725, 1436, 1445
BstMWI GCNNNNNNNGC 8 cut(s) 98, 893, 1299, 1406, 1444, 1742, 2492, 2954
BstNI CCWGG 2 cut(s) 1200, 2460
BstNSI RCATGY 4 cut(s) 112, 1216, 1228, 2824
BstPI GGTNACC 1 cut(s) 2938
BstSCI CCNGG 9 cut(s) 717, 859, 1038, 1198, 1976, 2240, 2241, 2458, 2794
BstSFI CTRYAG 4 cut(s) 441, 460, 1350, 2946
BstSLI GKGCMC 1 cut(s) 2814
BstSNI TACGTA 1 cut(s) 166
BstV1I GCAGC 8 cut(s) 22, 1032, 1393, 1732, 2498, 2606, 2711, 2775
BstV2I GAAGAC 4 cut(s) 261, 414, 616, 1813
BstX2I RGATCY 3 cut(s) 1028, 2344, 2653
BstXI CCANNNNNNTGG 1 cut(s) 2308
BstYI RGATCY 3 cut(s) 1028, 2344, 2653
BstZ17I GTATAC 1 cut(s) 169
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 6 cut(s) 86, 557, 890, 968, 1198, 2691
BsuTUI ATCGAT 1 cut(s) 705
BtgI CCRYGG 3 cut(s) 891, 2001, 2490
BtgZI GCGATG 3 cut(s) 264, 1283, 2354
BtrI CACGTC 1 cut(s) 2645
BtsI GCAGTG 2 cut(s) 2783, 2832
BtsIMutI CAGTG 4 cut(s) 393, 2783, 2832, 2931
CciI TCATGA 2 cut(s) 2878, 2967
CfoI GCGC 2 cut(s) 2061, 2624
Cfr13I GGNCC 6 cut(s) 205, 358, 555, 854, 1762, 2453
Cfr9I CCCGGG 1 cut(s) 2241
ClaI ATCGAT 1 cut(s) 705
CseI GACGC 4 cut(s) 457, 651, 2108, 2780
CsiI ACCWGGT 1 cut(s) 2458
Csp6I GTAC 6 cut(s) 175, 532, 864, 2095, 2463, 2991
CviQI GTAC 6 cut(s) 175, 532, 864, 2095, 2463, 2991
DdeI CTNAG 8 cut(s) 191, 285, 1726, 1816, 2073, 2854, 2934, 2958
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 7 cut(s) 752, 788, 827, 1467, 1866, 2208, 2426
EarI CTCTTC 7 cut(s) 752, 788, 827, 1467, 1866, 2208, 2426
EciI GGCGGA 1 cut(s) 442
Ecl136II GAGCTC 1 cut(s) 2965
Eco105I TACGTA 1 cut(s) 166
Eco130I CCWWGG 6 cut(s) 558, 891, 1257, 2001, 2490, 2869
Eco147I AGGCCT 3 cut(s) 968, 1198, 2691
Eco24I GRGCYC 1 cut(s) 2967
Eco31I GGTCTC 1 cut(s) 1445
Eco32I GATATC 1 cut(s) 2044
Eco47I GGWCC 5 cut(s) 205, 358, 854, 1762, 2453
Eco53kI GAGCTC 1 cut(s) 2965
Eco57I CTGAAG 4 cut(s) 608, 776, 1807, 3093
Eco88I CYCGRG 5 cut(s) 552, 1090, 1448, 2193, 2241
Eco91I GGTNACC 1 cut(s) 2938
EcoICRI GAGCTC 1 cut(s) 2965
EcoNI CCTNNNNNAGG 1 cut(s) 2463
EcoO109I RGGNCCY 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 2938
EcoRII CCWGG 2 cut(s) 1198, 2458
EcoRV GATATC 1 cut(s) 2044
EcoT14I CCWWGG 6 cut(s) 558, 891, 1257, 2001, 2490, 2869
EcoT22I ATGCAT 1 cut(s) 1516
EcoT38I GRGCYC 1 cut(s) 2967
ErhI CCWWGG 6 cut(s) 558, 891, 1257, 2001, 2490, 2869
FalI AAGNNNNNCTT 4 cut(s) 863, 895, 2045, 2077
FaqI GGGAC 3 cut(s) 344, 2655, 2744
FauI CCCGC 1 cut(s) 2720
FbaI TGATCA 1 cut(s) 765
FblI GTMKAC 2 cut(s) 168, 1096
FriOI GRGCYC 1 cut(s) 2967
FspBI CTAG 7 cut(s) 212, 246, 876, 936, 1178, 2132, 2870
GlaI GCGC 2 cut(s) 2060, 2623
HaeII RGCGCY 1 cut(s) 2062
HaeIII GGCC 6 cut(s) 86, 557, 890, 968, 1198, 2691
HapII CCGG 9 cut(s) 719, 749, 861, 1039, 1978, 2242, 2651, 2795, 3092
HgaI GACGC 4 cut(s) 457, 651, 2108, 2780
HhaI GCGC 2 cut(s) 2061, 2624
Hin6I GCGC 2 cut(s) 2059, 2622
HinP1I GCGC 2 cut(s) 2059, 2622
HincII GTYRAC 1 cut(s) 2679
HindII GTYRAC 1 cut(s) 2679
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 7 cut(s) 278, 583, 1043, 1505, 2168, 2251, 2844
HpaII CCGG 9 cut(s) 719, 749, 861, 1039, 1978, 2242, 2651, 2795, 3092
Hpy188I TCNGA 6 cut(s) 265, 588, 658, 1072, 1519, 1528
Hpy99I CGWCG 2 cut(s) 1112, 2124
HpyAV CCTTC 7 cut(s) 690, 958, 1409, 1636, 2080, 3022, 3043
HpyCH4III ACNGT 4 cut(s) 464, 2114, 2161, 2546
HpyCH4IV ACGT 6 cut(s) 165, 417, 613, 2184, 2644, 2659
HpyF10VI GCNNNNNNNGC 8 cut(s) 98, 893, 1299, 1406, 1444, 1742, 2492, 2954
HpyF3I CTNAG 8 cut(s) 191, 285, 1726, 1816, 2073, 2854, 2934, 2958
HpySE526I ACGT 6 cut(s) 165, 417, 613, 2184, 2644, 2659
HspAI GCGC 2 cut(s) 2059, 2622
Kpn2I TCCGGA 1 cut(s) 3091
Ksp22I TGATCA 1 cut(s) 765
Lsp1109I GCAGC 8 cut(s) 22, 1032, 1393, 1732, 2498, 2606, 2711, 2775
LweI GCATC 8 cut(s) 562, 711, 1393, 1523, 1721, 1757, 2323, 2971
MabI ACCWGGT 1 cut(s) 2458
MaeI CTAG 7 cut(s) 212, 246, 876, 936, 1178, 2132, 2870
MaeII ACGT 6 cut(s) 165, 417, 613, 2184, 2644, 2659
MaeIII GTNAC 6 cut(s) 104, 373, 455, 599, 839, 2938
MbiI CCGCTC 2 cut(s) 1174, 1767
MfeI CAATTG 1 cut(s) 1426
MflI RGATCY 3 cut(s) 1028, 2344, 2653
MhlI GDGCHC 5 cut(s) 717, 1337, 2814, 2933, 2967
MlyI GAGTC 3 cut(s) 577, 2162, 2260
MmeI TCCRAC 1 cut(s) 2886
Mph1103I ATGCAT 1 cut(s) 1516
MroI TCCGGA 1 cut(s) 3091
MseI TTAA 6 cut(s) 156, 179, 414, 912, 2355, 2913
MslI CAYNNNNRTG 8 cut(s) 113, 416, 1223, 2000, 2162, 2306, 2807, 2819
MspI CCGG 9 cut(s) 719, 749, 861, 1039, 1978, 2242, 2651, 2795, 3092
MspR9I CCNGG 9 cut(s) 719, 861, 1040, 1200, 1978, 2242, 2243, 2460, 2796
MunI CAATTG 1 cut(s) 1426
Mva1269I GAATGC 3 cut(s) 1278, 1489, 2874
MvaI CCWGG 2 cut(s) 1200, 2460
MwoI GCNNNNNNNGC 8 cut(s) 98, 893, 1299, 1406, 1444, 1742, 2492, 2954
NciI CCSGG 7 cut(s) 719, 861, 1040, 1978, 2242, 2243, 2796
NcoI CCATGG 3 cut(s) 891, 2001, 2490
NlaIV GGNNCC 7 cut(s) 207, 360, 747, 1030, 2299, 2346, 2655
NmeAIII GCCGAG 2 cut(s) 62, 1254
NmuCI GTSAC 1 cut(s) 2938
NsiI ATGCAT 1 cut(s) 1516
NspI RCATGY 4 cut(s) 112, 1216, 1228, 2824
NspV TTCGAA 2 cut(s) 331, 405
PaeR7I CTCGAG 2 cut(s) 1448, 2193
PagI TCATGA 2 cut(s) 2878, 2967
PceI AGGCCT 3 cut(s) 968, 1198, 2691
PciI ACATGT 3 cut(s) 108, 1224, 2820
PctI GAATGC 3 cut(s) 1278, 1489, 2874
PfeI GAWTC 4 cut(s) 278, 1043, 1505, 2844
PleI GAGTC 3 cut(s) 577, 2162, 2259
PpsI GAGTC 3 cut(s) 577, 2162, 2259
Ppu21I YACGTR 1 cut(s) 166
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 3 cut(s) 108, 1224, 2820
PsiI TTATAA 2 cut(s) 524, 1607
Psp124BI GAGCTC 1 cut(s) 2967
Psp1406I AACGTT 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 1198, 2458
PspEI GGTNACC 1 cut(s) 2938
PspGI CCWGG 2 cut(s) 1198, 2458
PspN4I GGNNCC 7 cut(s) 207, 360, 747, 1030, 2299, 2346, 2655
PspPI GGNCC 6 cut(s) 205, 358, 555, 854, 1762, 2453
PspPPI RGGWCCY 1 cut(s) 358
PspXI VCTCGAGB 1 cut(s) 2193
PstI CTGCAG 2 cut(s) 1354, 2950
PsuI RGATCY 3 cut(s) 1028, 2344, 2653
RsaI GTAC 6 cut(s) 176, 533, 865, 2096, 2464, 2992
RsaNI GTAC 6 cut(s) 175, 532, 864, 2095, 2463, 2991
RseI CAYNNNNRTG 8 cut(s) 113, 416, 1223, 2000, 2162, 2306, 2807, 2819
SacI GAGCTC 1 cut(s) 2967
SaqAI TTAA 6 cut(s) 156, 179, 414, 912, 2355, 2913
Sau96I GGNCC 6 cut(s) 205, 358, 555, 854, 1762, 2453
SchI GAGTC 3 cut(s) 577, 2162, 2260
ScrFI CCNGG 9 cut(s) 719, 861, 1040, 1200, 1978, 2242, 2243, 2460, 2796
SduI GDGCHC 5 cut(s) 717, 1337, 2814, 2933, 2967
SexAI ACCWGGT 1 cut(s) 2458
SfaNI GCATC 8 cut(s) 562, 711, 1393, 1523, 1721, 1757, 2323, 2971
SfcI CTRYAG 4 cut(s) 441, 460, 1350, 2946
Sfr274I CTCGAG 2 cut(s) 1448, 2193
SfuI TTCGAA 2 cut(s) 331, 405
SinI GGWCC 5 cut(s) 205, 358, 854, 1762, 2453
SlaI CTCGAG 2 cut(s) 1448, 2193
SmaI CCCGGG 1 cut(s) 2243
SmiMI CAYNNNNRTG 8 cut(s) 113, 416, 1223, 2000, 2162, 2306, 2807, 2819
SmlI CTYRAG 8 cut(s) 710, 1433, 1448, 1659, 1958, 2193, 2504, 3017
SmoI CTYRAG 8 cut(s) 710, 1433, 1448, 1659, 1958, 2193, 2504, 3017
SnaBI TACGTA 1 cut(s) 166
SpeI ACTAGT 1 cut(s) 935
SseBI AGGCCT 3 cut(s) 968, 1198, 2691
SsiI CCGC 7 cut(s) 427, 481, 887, 1174, 1765, 1913, 2727
SspI AATATT 2 cut(s) 17, 916
SspMI CTAG 7 cut(s) 212, 246, 876, 936, 1178, 2132, 2870
SstI GAGCTC 1 cut(s) 2967
StuI AGGCCT 3 cut(s) 968, 1198, 2691
StyD4I CCNGG 9 cut(s) 717, 859, 1038, 1198, 1976, 2240, 2241, 2458, 2794
StyI CCWWGG 6 cut(s) 558, 891, 1257, 2001, 2490, 2869
TaaI ACNGT 4 cut(s) 464, 2114, 2161, 2546
TaiI ACGT 6 cut(s) 168, 420, 616, 2187, 2647, 2662
TaqII GACCGA 1 cut(s) 610
TauI GCSGC 3 cut(s) 483, 890, 1177
TfiI GAWTC 4 cut(s) 278, 1043, 1505, 2844
Tru1I TTAA 6 cut(s) 156, 179, 414, 912, 2355, 2913
Tru9I TTAA 6 cut(s) 156, 179, 414, 912, 2355, 2913
TscAI CASTG 4 cut(s) 400, 2790, 2839, 2938
TseFI GTSAC 1 cut(s) 2938
TseI GCWGC 8 cut(s) 10, 1020, 1406, 1745, 2486, 2619, 2724, 2788
Tsp45I GTSAC 1 cut(s) 2938
TspDTI ATGAA 7 cut(s) 143, 267, 1410, 1485, 2692, 2867, 2984
TspGWI ACGGA 1 cut(s) 1021
TspMI CCCGGG 1 cut(s) 2241
TspRI CASTG 4 cut(s) 400, 2790, 2839, 2938
VneI GTGCAC 1 cut(s) 2810
VpaK11BI GGWCC 5 cut(s) 205, 358, 854, 1762, 2453
XagI CCTNNNNNAGG 1 cut(s) 2463
XapI RAATTY 3 cut(s) 630, 1521, 1644
XceI RCATGY 4 cut(s) 112, 1216, 1228, 2824
XhoI CTCGAG 2 cut(s) 1448, 2193
XmaI CCCGGG 1 cut(s) 2241
XmaJI CCTAGG 1 cut(s) 2869
XmiI GTMKAC 2 cut(s) 168, 1096
XspI CTAG 7 cut(s) 212, 246, 876, 936, 1178, 2132, 2870
Zsp2I ATGCAT 1 cut(s) 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.