FvH4_1g16231

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002989
Physical Location & Seq
Reverse (-)
9314129 .. 9315283
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16231.t1

Sequence Viewer

Length: 615 bp
ATGAAGCATGCCGTTGATCATAAAAAAGACACAAAGATATTGGAGGACCATAACCTCAAGTCTGATTTTCAAATTGGTTTATCCCCAGATGCTAAAATCAAGCGGCAAAAAGCCCTAAAAATTGAAGAACACAAAGAAGCCACTATTGGTGTACAACAACGAGGTTTGTATCGTCAAAATTATGCTCCTTCCAATCTCCCACCAATCCCTCGTCTACACAGCCTGATTCAAATTTGTAGCAAGCCATATGAGAAGCAATTAACAAGGAGCGATGTGAAGGACGACCAAGCAAGGCTAGCTATGAGCAAAGAGCATGTTACAAATCACCTAATTCCATTGTTGAATGAGAATGAAGATCTATACCAAGGAATCCATAACGCTATGAAAGTTATAACTTATGATTTGGCGGGTAAGGAGTATCCCATGGCGTTCAAAATTTGGGCTTCCAAGATTCATGTCTTGACTGGAGGATGGAAAACTTTTTGCCATGAACATGATTTGGTTGAGATCCAAGACTTTGTTAGGGTGTGGATGTTTCGGAATGTTGCAACTGGCAACCTTTGCTTTGTGATCGATCGAGTCGAGAGGAATGCTGGGATGGCTCAACAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

205

Amino Acids

23.54

Weight (kDa)

8.87

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 84 - 175 4.2e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AccI GTMKAC 1 cut(s) 214
AciI CCGC 2 cut(s) 103, 407
AclWI GGATC 1 cut(s) 502
AcsI RAATTY 2 cut(s) 231, 435
AfaI GTAC 1 cut(s) 153
AgsI TTSAA 5 cut(s) 71, 125, 230, 343, 433
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
AlwI GGATC 1 cut(s) 502
ApoI RAATTY 2 cut(s) 231, 435
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 317
AsuNHI GCTAGC 1 cut(s) 295
AvaII GGWCC 1 cut(s) 46
BccI CCATC 2 cut(s) 465, 592
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciVI GTATCC 1 cut(s) 429
BclI TGATCA 1 cut(s) 16
BfaI CTAG 1 cut(s) 296
BfuI GTATCC 1 cut(s) 429
BglII AGATCT 1 cut(s) 355
BisI GCNGC 1 cut(s) 104
BlsI GCNGC 1 cut(s) 105
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmsI GCATC 1 cut(s) 79
BmtI GCTAGC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 41
Bsa29I ATCGAT 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 364, 423
Bse1I ACTGG 2 cut(s) 469, 556
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 2 cut(s) 364, 423
BseGI GGATG 3 cut(s) 476, 537, 603
BseNI ACTGG 2 cut(s) 469, 556
BseYI CCCAGC 1 cut(s) 593
Bsh1285I CGRYCG 1 cut(s) 577
BshVI ATCGAT 1 cut(s) 573
BsiEI CGRYCG 1 cut(s) 577
BsmI GAATGC 1 cut(s) 595
Bsp1407I TGTACA 1 cut(s) 151
Bsp143I GATC 5 cut(s) 16, 355, 507, 570, 574
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 2 cut(s) 103, 407
BspDI ATCGAT 1 cut(s) 573
BspOI GCTAGC 1 cut(s) 299
BspPI GGATC 1 cut(s) 502
BsrGI TGTACA 1 cut(s) 151
BsrI ACTGG 2 cut(s) 469, 556
BssECI CCNNGG 2 cut(s) 364, 423
BssMI GATC 5 cut(s) 16, 355, 507, 570, 574
BssT1I CCWWGG 2 cut(s) 364, 423
BstAPI GCANNNNNTGC 1 cut(s) 561
BstAUI TGTACA 1 cut(s) 151
BstC8I GCNNGC 3 cut(s) 9, 242, 297
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 3 cut(s) 476, 537, 603
BstKTI GATC 5 cut(s) 19, 358, 510, 573, 577
BstMBI GATC 5 cut(s) 16, 355, 507, 570, 574
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 3 cut(s) 296, 561, 599
BstNSI RCATGY 2 cut(s) 11, 317
BstX2I RGATCY 2 cut(s) 355, 507
BstYI RGATCY 2 cut(s) 355, 507
Bsu15I ATCGAT 1 cut(s) 573
BsuI GTATCC 1 cut(s) 429
BsuTUI ATCGAT 1 cut(s) 573
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 3 cut(s) 476, 537, 603
Cac8I GCNNGC 3 cut(s) 9, 242, 297
Cfr13I GGNCC 1 cut(s) 46
ClaI ATCGAT 1 cut(s) 573
Csp6I GTAC 1 cut(s) 152
CviAII CATG 6 cut(s) 8, 314, 424, 455, 488, 494
CviJI RGCY 8 cut(s) 113, 140, 222, 244, 295, 299, 443, 602
CviKI_1 RGCY 8 cut(s) 113, 140, 222, 244, 295, 299, 443, 602
CviQI GTAC 1 cut(s) 152
DpnI GATC 5 cut(s) 18, 357, 509, 572, 576
DpnII GATC 5 cut(s) 16, 355, 507, 570, 574
Eco130I CCWWGG 2 cut(s) 364, 423
Eco47I GGWCC 1 cut(s) 46
EcoT14I CCWWGG 2 cut(s) 364, 423
ErhI CCWWGG 2 cut(s) 364, 423
FaeI CATG 6 cut(s) 11, 317, 427, 458, 491, 497
FatI CATG 6 cut(s) 7, 313, 423, 454, 487, 493
FauI CCCGC 1 cut(s) 400
FauNDI CATATG 1 cut(s) 247
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 1 cut(s) 104
FokI GGATG 3 cut(s) 483, 544, 610
Fsp4HI GCNGC 1 cut(s) 104
FspBI CTAG 1 cut(s) 296
GluI GCNGC 1 cut(s) 104
GsaI CCCAGC 1 cut(s) 597
GsuI CTGGAG 1 cut(s) 486
Hin1II CATG 6 cut(s) 11, 317, 427, 458, 491, 497
HinfI GANTC 4 cut(s) 226, 369, 451, 579
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 2 cut(s) 152, 215
Hpy188I TCNGA 2 cut(s) 64, 540
Hpy188III TCNNGA 2 cut(s) 460, 583
Hpy8I GTNNAC 2 cut(s) 152, 215
HpyAV CCTTC 2 cut(s) 198, 271
HpyCH4V TGCA 1 cut(s) 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 561, 599
Hsp92II CATG 6 cut(s) 11, 317, 427, 458, 491, 497
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 355, 507, 570, 574
LmnI GCTCC 2 cut(s) 190, 267
LpnPI CCDG 5 cut(s) 99, 236, 450, 537, 579
LweI GCATC 1 cut(s) 79
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 316
MalI GATC 5 cut(s) 18, 357, 509, 572, 576
MboI GATC 5 cut(s) 16, 355, 507, 570, 574
MboII GAAGA 2 cut(s) 137, 365
MflI RGATCY 2 cut(s) 355, 507
MluCI AATT 7 cut(s) 72, 120, 178, 231, 257, 330, 435
MlyI GAGTC 1 cut(s) 588
MnlI CCTC 6 cut(s) 37, 65, 155, 219, 461, 579
MseI TTAA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 595
MwoI GCNNNNNNNGC 3 cut(s) 296, 561, 599
NcoI CCATGG 1 cut(s) 423
NdeI CATATG 1 cut(s) 247
NdeII GATC 5 cut(s) 16, 355, 507, 570, 574
NheI GCTAGC 1 cut(s) 295
NlaIII CATG 6 cut(s) 11, 317, 427, 458, 491, 497
NspI RCATGY 2 cut(s) 11, 317
PaeI GCATGC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 1 cut(s) 579
PctI GAATGC 1 cut(s) 595
PfeI GAWTC 3 cut(s) 226, 369, 451
PkrI GCNGC 1 cut(s) 105
Ple19I CGATCG 1 cut(s) 577
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
PsiI TTATAA 1 cut(s) 392
PspFI CCCAGC 1 cut(s) 593
PspPI GGNCC 1 cut(s) 46
PsuI RGATCY 2 cut(s) 355, 507
PvuI CGATCG 1 cut(s) 577
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
RseI CAYNNNNRTG 1 cut(s) 492
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 1 cut(s) 104
Sau3AI GATC 5 cut(s) 16, 355, 507, 570, 574
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 588
SetI ASST 5 cut(s) 57, 166, 301, 330, 561
SfaNI GCATC 1 cut(s) 79
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SphI GCATGC 1 cut(s) 11
Sse9I AATT 7 cut(s) 72, 120, 178, 231, 257, 330, 435
SsiI CCGC 2 cut(s) 103, 407
SspMI CTAG 1 cut(s) 296
StyI CCWWGG 2 cut(s) 364, 423
TaqI TCGA 3 cut(s) 573, 577, 582
TasI AATT 7 cut(s) 72, 120, 178, 231, 257, 330, 435
TatI WGTACW 1 cut(s) 151
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 3 cut(s) 226, 369, 451
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TspDTI ATGAA 5 cut(s) 17, 366, 398, 443, 504
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 2 cut(s) 231, 435
XceI RCATGY 2 cut(s) 11, 317
XmiI GTMKAC 1 cut(s) 214
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.