MD00G1107900.v1.1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002989
Physical Location & Seq
Reverse (-)
22559806 .. 22560624
819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1107900.v1.1.491

Sequence Viewer

Length: 819 bp
ATGATCTTGGGAGAGAGATATACGTCAGTCATGGTTGATCAATACTGTGAAGACCCCAGTCACGAAGACAAAGAGATGGCTACGACGACTGAGGGTGCTAATCTTCTTCTATACCTAAAACGCAAACTTTTGAGTGCCGAAACAGCCCGAAAATTGGAGTACATGAAATACAAAGTTCTTTCTCAAAATGGGTTGTATTCGTATGCAAGAACCAAGAGAAAAGCACCACGAATCGAAGTGGATGAAGCCCCGCTACCCCAAGGAGCCATTAGTGGTCCTTCTGCACCCCAAGAAGCCATTGAGGGTGCTTCTCCACGAGGACGACGCTTGCCGGGAGAACCACAATTACAAGTAATTCAAGGCTTGTTCTATGGAAAATATGCCCCTCCTGATCTCCCACCAGTCCCAAGTCTACGGTCCTTAATTCAAGTTCACAGCAAGCCTTTTGAGAAACAACTGACAAGTAGTGATGTGAGGGATGACCAATCTAGGCTTTCGATGTGCAAAAAAGATGTCGAAAATCACATCTTCCCGCTGTTGAAGGACGACGAAGACCCTAACCGAGGCATTCCTGTAACAACTTATGACATGGGCGGTAATAAATACCCTATGGTGTTCAAAACTTGGGTGTCCAAGACTCATGTCCTCACTGGAGGTTGGAAAAGTTTTTGTCATGATCGCGGATTGGTTGAGAAGATTGATTTTGTGACGGTGTGGGTTTTTCGGAATGTGGTGAATGGAAGCCTTTGCTTTGCCATCAATTCAAGAAGGTTTCCGGCCGTGTCTGAAGCCATCAAGAAAAGAAGAAGGCAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

273

Amino Acids

30.86

Weight (kDa)

9.3

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 149 - 252 4.6e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 412
AccII CGCG 1 cut(s) 681
AciI CCGC 4 cut(s) 251, 533, 594, 681
AcoI YGGCCR 1 cut(s) 777
AcuI CTGAAG 1 cut(s) 807
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 154, 563
AgsI TTSAA 5 cut(s) 359, 428, 541, 619, 765
AoxI GGCC 1 cut(s) 777
ArsI GACNNNNNNTTYG 2 cut(s) 498, 530
AspS9I GGNCC 2 cut(s) 275, 417
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 1 cut(s) 745
AvaII GGWCC 2 cut(s) 275, 417
BarI GAAGNNNNNNTAC 2 cut(s) 237, 269
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 3 cut(s) 57, 72, 558
BccI CCATC 3 cut(s) 70, 764, 800
BceAI ACGGC 1 cut(s) 764
BclI TGATCA 1 cut(s) 37
BcnI CCSGG 1 cut(s) 333
BfaI CTAG 1 cut(s) 489
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 2 cut(s) 275, 417
BmgT120I GGNCC 2 cut(s) 275, 417
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 333
BmrI ACTGGG 1 cut(s) 51
BmuI ACTGGG 1 cut(s) 51
BoxI GACNNNNGTC 2 cut(s) 57, 641
BpiI GAAGAC 3 cut(s) 57, 72, 558
BpmI CTGGAG 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 259, 562
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 563
Bse1I ACTGG 3 cut(s) 57, 401, 655
BseDI CCNNGG 2 cut(s) 259, 562
BseGI GGATG 2 cut(s) 247, 484
BseLI CCNNNNNNNGG 2 cut(s) 154, 563
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 3 cut(s) 57, 401, 655
BseX3I CGGCCG 1 cut(s) 777
BsgI GTGCAG 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 681
Bsh1285I CGRYCG 1 cut(s) 780
BshFI GGCC 1 cut(s) 779
BsiEI CGRYCG 1 cut(s) 780
BsiSI CCGG 2 cut(s) 332, 776
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 154, 563
BsmFI GGGAC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 1 cut(s) 779
Bsp143I GATC 4 cut(s) 3, 37, 391, 676
BspACI CCGC 4 cut(s) 251, 533, 594, 681
BspANI GGCC 1 cut(s) 779
BspCNI CTCAG 1 cut(s) 82
BspFNI CGCG 1 cut(s) 681
BspHI TCATGA 1 cut(s) 673
BspLI GGNNCC 1 cut(s) 265
BsrI ACTGG 3 cut(s) 57, 401, 655
BssECI CCNNGG 2 cut(s) 259, 562
BssMI GATC 4 cut(s) 3, 37, 391, 676
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 259
Bst2BI CACGAG 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 47, 417, 712
BstC8I GCNNGC 2 cut(s) 329, 440
BstDEI CTNAG 1 cut(s) 90
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 247, 484
BstFNI CGCG 1 cut(s) 681
BstKTI GATC 4 cut(s) 6, 40, 394, 679
BstMBI GATC 4 cut(s) 3, 37, 391, 676
BstMCI CGRYCG 1 cut(s) 780
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstPAI GACNNNNGTC 2 cut(s) 57, 641
BstSCI CCNGG 1 cut(s) 331
BstUI CGCG 1 cut(s) 681
BstV2I GAAGAC 3 cut(s) 57, 72, 558
BstZI CGGCCG 1 cut(s) 777
BsuRI GGCC 1 cut(s) 779
BtsCI GGATG 2 cut(s) 247, 484
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 2 cut(s) 329, 440
CciI TCATGA 1 cut(s) 673
Cfr13I GGNCC 2 cut(s) 275, 417
CseI GACGC 1 cut(s) 333
Csp6I GTAC 1 cut(s) 160
CviAII CATG 5 cut(s) 31, 163, 589, 641, 674
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 90
DpnI GATC 4 cut(s) 5, 39, 393, 678
DpnII GATC 4 cut(s) 3, 37, 391, 676
EaeI YGGCCR 1 cut(s) 777
EagI CGGCCG 1 cut(s) 777
EclXI CGGCCG 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 275, 417
Eco52I CGGCCG 1 cut(s) 777
Eco57I CTGAAG 1 cut(s) 807
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 5 cut(s) 34, 166, 592, 644, 677
FaqI GGGAC 1 cut(s) 389
FatI CATG 5 cut(s) 30, 162, 588, 640, 673
FauI CCCGC 2 cut(s) 258, 540
FbaI TGATCA 1 cut(s) 37
FblI GTMKAC 1 cut(s) 412
FokI GGATG 2 cut(s) 254, 491
FspBI CTAG 1 cut(s) 489
GsuI CTGGAG 1 cut(s) 672
HaeIII GGCC 1 cut(s) 779
HapII CCGG 2 cut(s) 332, 776
HgaI GACGC 1 cut(s) 333
Hin1II CATG 5 cut(s) 34, 166, 592, 644, 677
HinfI GANTC 2 cut(s) 231, 637
HpaII CCGG 2 cut(s) 332, 776
HphI GGTGA 1 cut(s) 745
Hpy166II GTNNAC 2 cut(s) 413, 433
Hpy188I TCNGA 2 cut(s) 726, 787
Hpy188III TCNNGA 5 cut(s) 62, 389, 674, 765, 796
Hpy8I GTNNAC 2 cut(s) 413, 433
Hpy99I CGWCG 3 cut(s) 88, 327, 551
HpyAV CCTTC 4 cut(s) 288, 535, 762, 801
HpyCH4III ACNGT 3 cut(s) 47, 417, 712
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 206, 284, 504
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 5 cut(s) 34, 166, 592, 644, 677
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 4 cut(s) 3, 37, 391, 676
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 7 cut(s) 70, 345, 402, 414, 585, 636, 789
MaeI CTAG 1 cut(s) 489
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 3 cut(s) 59, 574, 706
MalI GATC 4 cut(s) 5, 39, 393, 678
MboI GATC 4 cut(s) 3, 37, 391, 676
MboII GAAGA 8 cut(s) 62, 77, 95, 98, 520, 563, 706, 816
MluCI AATT 5 cut(s) 152, 344, 354, 423, 760
MlyI GAGTC 1 cut(s) 631
MmeI TCCRAC 1 cut(s) 638
MnlI CCTC 8 cut(s) 85, 295, 311, 396, 468, 557, 647, 656
MseI TTAA 1 cut(s) 422
MspA1I CMGCKG 1 cut(s) 535
MspI CCGG 2 cut(s) 332, 776
MspR9I CCNGG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 567
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 1 cut(s) 143
NciI CCSGG 1 cut(s) 333
NdeII GATC 4 cut(s) 3, 37, 391, 676
NlaIII CATG 5 cut(s) 34, 166, 592, 644, 677
NlaIV GGNNCC 1 cut(s) 265
NmuCI GTSAC 2 cut(s) 59, 706
PagI TCATGA 1 cut(s) 673
PctI GAATGC 1 cut(s) 567
PfeI GAWTC 1 cut(s) 231
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
PshAI GACNNNNGTC 2 cut(s) 57, 641
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 275, 417
PsrI GAACNNNNNNTAC 2 cut(s) 330, 362
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 422
Sau3AI GATC 4 cut(s) 3, 37, 391, 676
Sau96I GGNCC 2 cut(s) 275, 417
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 1 cut(s) 333
SetI ASST 4 cut(s) 26, 117, 658, 773
SinI GGWCC 2 cut(s) 275, 417
Sse9I AATT 5 cut(s) 152, 344, 354, 423, 760
SsiI CCGC 4 cut(s) 251, 533, 594, 681
SspMI CTAG 1 cut(s) 489
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 3 cut(s) 47, 417, 712
TaiI ACGT 1 cut(s) 26
TaqI TCGA 3 cut(s) 234, 497, 516
TasI AATT 5 cut(s) 152, 344, 354, 423, 760
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 2 cut(s) 59, 706
Tsp45I GTSAC 2 cut(s) 59, 706
TspDTI ATGAA 2 cut(s) 179, 258
TspRI CASTG 1 cut(s) 655
VpaK11BI GGWCC 2 cut(s) 275, 417
XagI CCTNNNNNAGG 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 412
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.