RLG00000004342

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002989
Physical Location & Seq
Forward (+)
58470497 .. 58470880
384 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004342

Sequence Viewer

Length: 384 bp
ATGCCCCTCCCGGAGAAGCAATTAACATTTAGTGATGTGAAGGATGACCAATCAAGGCTAGCTATGAGCAAAGAGGATGTCCAAAAGCGCCTAATGCCATTGTTGAATGAGAATGAAGATCTTAGACGAGGAATCCAAGTCACAACTTATGATTTGGATGGTAAAGAGTATCCCATGGTGTTCAAAATTTGGGTGGAAAAGATTCATGTCTTGACCGGAGGATGGAAAGCTTTTTGCCATGATCATAAGCTGGTTGAGAAGCAAGATTTTGTGACCGTATGGATGTTTCGAAATGCTGCAACCGGTAGCCTTTGCTTTGTAATTGATTCGAGAAGGTTGCCAGTGCTTGAAACCATCAAGAGAAGAAGAACAAGGCGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

128

Amino Acids

15.05

Weight (kDa)

9.39

Isoelectric Point (pI)

75.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 18 - 106 5.2e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 222
AgeI ACCGGT 1 cut(s) 302
AgsI TTSAA 3 cut(s) 106, 184, 350
AluBI AGCT 3 cut(s) 62, 230, 250
AluI AGCT 3 cut(s) 62, 230, 250
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 1 cut(s) 186
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
AsiGI ACCGGT 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 11
AsuII TTCGAA 1 cut(s) 289
AsuNHI GCTAGC 1 cut(s) 58
BbvI GCAGC 1 cut(s) 283
BccI CCATC 3 cut(s) 152, 216, 362
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciVI GTATCC 1 cut(s) 180
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 1 cut(s) 11
BfaI CTAG 1 cut(s) 59
BfoI RGCGCY 1 cut(s) 91
BfuI GTATCC 1 cut(s) 180
BglII AGATCT 1 cut(s) 118
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 11
BmrFI CCNGG 1 cut(s) 11
BmtI GCTAGC 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 289
BpuMI CCSGG 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 174
BsaWI WCCGGW 2 cut(s) 215, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 341
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 5 cut(s) 49, 82, 163, 227, 288
BseLI CCNNNNNNNGG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 283
BshTI ACCGGT 1 cut(s) 302
BsiSI CCGG 3 cut(s) 11, 216, 303
BslI CCNNNNNNNGG 1 cut(s) 222
Bsp119I TTCGAA 1 cut(s) 289
Bsp143I GATC 2 cut(s) 118, 241
Bsp19I CCATGG 1 cut(s) 174
BspOI GCTAGC 1 cut(s) 62
BspT104I TTCGAA 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 1 cut(s) 341
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 2 cut(s) 118, 241
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 1 cut(s) 277
BstBI TTCGAA 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 5 cut(s) 49, 82, 163, 227, 288
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 2 cut(s) 121, 244
BstMBI GATC 2 cut(s) 118, 241
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 283
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuI GTATCC 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 5 cut(s) 49, 82, 163, 227, 288
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 1 cut(s) 302
CspAI ACCGGT 1 cut(s) 302
CviAII CATG 3 cut(s) 175, 206, 239
CviJI RGCY 5 cut(s) 58, 62, 230, 250, 309
CviKI_1 RGCY 5 cut(s) 58, 62, 230, 250, 309
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 120, 243
DpnII GATC 2 cut(s) 118, 241
Eco130I CCWWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 3 cut(s) 178, 209, 242
FaiI YATR 7 cut(s) 65, 150, 176, 207, 240, 246, 280
FatI CATG 3 cut(s) 174, 205, 238
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 5 cut(s) 56, 89, 170, 234, 295
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 89
GluI GCNGC 1 cut(s) 297
HaeII RGCGCY 1 cut(s) 91
HapII CCGG 3 cut(s) 11, 216, 303
HhaI GCGC 1 cut(s) 90
Hin1II CATG 3 cut(s) 178, 209, 242
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 3 cut(s) 132, 202, 326
HpaII CCGG 3 cut(s) 11, 216, 303
Hpy188III TCNNGA 3 cut(s) 211, 330, 358
HpyAV CCTTC 2 cut(s) 34, 327
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 1 cut(s) 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
HpyF3I CTNAG 1 cut(s) 122
Hsp92II CATG 3 cut(s) 178, 209, 242
HspAI GCGC 1 cut(s) 88
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 2 cut(s) 118, 241
LpnPI CCDG 5 cut(s) 24, 229, 236, 316, 354
Lsp1109I GCAGC 1 cut(s) 283
MaeI CTAG 1 cut(s) 59
MaeIII GTNAC 2 cut(s) 139, 271
MalI GATC 2 cut(s) 120, 243
MboI GATC 2 cut(s) 118, 241
MboII GAAGA 3 cut(s) 128, 375, 378
MflI RGATCY 1 cut(s) 118
MluCI AATT 4 cut(s) 20, 186, 321, 379
MnlI CCTC 4 cut(s) 17, 67, 122, 212
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 2 cut(s) 23, 382
MspI CCGG 3 cut(s) 11, 216, 303
MspR9I CCNGG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 94
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 1 cut(s) 174
NdeII GATC 2 cut(s) 118, 241
NheI GCTAGC 1 cut(s) 58
NlaIII CATG 3 cut(s) 178, 209, 242
NmuCI GTSAC 2 cut(s) 139, 271
NspV TTCGAA 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 132, 202, 326
PfoI TCCNGGA 1 cut(s) 9
PinAI ACCGGT 1 cut(s) 302
PkrI GCNGC 1 cut(s) 298
PsuI RGATCY 1 cut(s) 118
SaqAI TTAA 2 cut(s) 23, 382
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 2 cut(s) 118, 241
ScrFI CCNGG 1 cut(s) 11
SetI ASST 4 cut(s) 64, 232, 252, 338
SfuI TTCGAA 1 cut(s) 289
Sse9I AATT 4 cut(s) 20, 186, 321, 379
SspMI CTAG 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 2 cut(s) 289, 329
TasI AATT 4 cut(s) 20, 186, 321, 379
TfiI GAWTC 3 cut(s) 132, 202, 326
Tru1I TTAA 2 cut(s) 23, 382
Tru9I TTAA 2 cut(s) 23, 382
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 2 cut(s) 139, 271
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 2 cut(s) 139, 271
TspDTI ATGAA 2 cut(s) 129, 194
TspRI CASTG 1 cut(s) 348
XapI RAATTY 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.