MD15G1316300.v1.1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002989
Physical Location & Seq
Forward (+)
32449135 .. 32449912
778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1316300.v1.1.491

Sequence Viewer

Length: 570 bp
ATGATGTTGGGAGAACCACAACTACATGGAATTCAAGGCTTGTTCTATGGAAACTATGCCCCTCCTGATCTCCCACCAGTCCCAAGTCTACGACCCTTGATTCAAGTTCATAGCAAGCCTTTTGAGAAACAACTGACAAGTAGTGATGTGAGGGATGACCAATCTAGGCTCTCGATGAGCAAAGAAGATGTCGAAAATCACATCTTCCCATTGTTTAAGGACGGCGAAGACCCTAACCAAGGCGTTCATGTCACAATTTATGACATGGATGGTAAAGAGTACCCCATGGTGTTCAAAACTTGGATGTCCAAGATTAATGTCCTCACTGGAGGCTGGAACAGTTTTCGTCAGGATCGCGGGTTGGTTGAGAAGATTGATTTTGTGACAGTGTGGGTTTTTCGGAATGTGTTGAATGGGAGCCTTTGCTTCGCCATCAATTCAAGAAGGTTTCCGGCAGTGTCCGAAGCCATCAAGAGAAGAAAAAGGCAGTACCGAGACAGCGGACCGCCGTTACAAGTTTCTTCTAGTTCGGTCCAAATCATTGGGGTTGCTTTTTCCAATAAACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

190

Amino Acids

21.54

Weight (kDa)

9.24

Isoelectric Point (pI)

58.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 41 - 143 1.3e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 88
AccII CGCG 1 cut(s) 357
AciI CCGC 3 cut(s) 357, 501, 506
AclI AACGTT 1 cut(s) 565
AclWI GGATC 1 cut(s) 360
AcsI RAATTY 1 cut(s) 30
AfaI GTAC 2 cut(s) 281, 491
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 5 cut(s) 35, 104, 295, 412, 441
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 1 cut(s) 360
ApoI RAATTY 1 cut(s) 30
ArsI GACNNNNNNTTYG 2 cut(s) 174, 206
AseI ATTAAT 1 cut(s) 315
AspS9I GGNCC 2 cut(s) 503, 532
AvaII GGWCC 2 cut(s) 503, 532
BbsI GAAGAC 1 cut(s) 234
BccI CCATC 3 cut(s) 263, 440, 476
BceAI ACGGC 2 cut(s) 238, 493
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 2 cut(s) 165, 525
Bme18I GGWCC 2 cut(s) 503, 532
BmgT120I GGNCC 2 cut(s) 503, 532
BmiI GGNNCC 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 238, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse1I ACTGG 2 cut(s) 77, 331
BseDI CCNNGG 2 cut(s) 238, 285
BseGI GGATG 3 cut(s) 160, 274, 309
BseLI CCNNNNNNNGG 1 cut(s) 239
BseNI ACTGG 2 cut(s) 77, 331
Bsh1236I CGCG 1 cut(s) 357
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 489
BsmFI GGGAC 1 cut(s) 65
Bsp143I GATC 2 cut(s) 67, 352
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 3 cut(s) 357, 501, 506
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 419
BspPI GGATC 1 cut(s) 360
BsrI ACTGG 2 cut(s) 77, 331
BssECI CCNNGG 2 cut(s) 238, 285
BssMI GATC 2 cut(s) 67, 352
BssT1I CCWWGG 2 cut(s) 238, 285
Bst4CI ACNGT 2 cut(s) 341, 388
BstC8I GCNNGC 1 cut(s) 116
BstDSI CCRYGG 1 cut(s) 285
BstENI CCTNNNNNAGG 1 cut(s) 237
BstF5I GGATG 3 cut(s) 160, 274, 309
BstFNI CGCG 1 cut(s) 357
BstKTI GATC 2 cut(s) 70, 355
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 2 cut(s) 67, 352
BstUI CGCG 1 cut(s) 357
BstV2I GAAGAC 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 542
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 3 cut(s) 160, 274, 309
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 324, 393, 462
Cac8I GCNNGC 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 503, 532
CpoI CGGWCCG 1 cut(s) 503
Csp6I GTAC 2 cut(s) 280, 490
CspI CGGWCCG 1 cut(s) 503
CviAII CATG 4 cut(s) 26, 248, 265, 286
CviJI RGCY 6 cut(s) 39, 118, 169, 333, 420, 467
CviKI_1 RGCY 6 cut(s) 39, 118, 169, 333, 420, 467
CviQI GTAC 2 cut(s) 280, 490
DpnI GATC 2 cut(s) 69, 354
DpnII GATC 2 cut(s) 67, 352
Eco130I CCWWGG 2 cut(s) 238, 285
Eco47I GGWCC 2 cut(s) 503, 532
EcoNI CCTNNNNNAGG 1 cut(s) 237
EcoRI GAATTC 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 238, 285
ErhI CCWWGG 2 cut(s) 238, 285
FaeI CATG 4 cut(s) 29, 251, 268, 289
FaiI YATR 8 cut(s) 27, 48, 57, 111, 249, 261, 266, 287
FaqI GGGAC 1 cut(s) 65
FatI CATG 4 cut(s) 25, 247, 264, 285
FauI CCCGC 1 cut(s) 350
FblI GTMKAC 1 cut(s) 88
FokI GGATG 3 cut(s) 167, 281, 316
FspBI CTAG 2 cut(s) 165, 525
GsuI CTGGAG 1 cut(s) 348
HapII CCGG 1 cut(s) 452
Hin1II CATG 4 cut(s) 29, 251, 268, 289
HinfI GANTC 1 cut(s) 100
HpaII CCGG 1 cut(s) 452
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 2 cut(s) 402, 463
Hpy188III TCNNGA 5 cut(s) 65, 172, 350, 441, 472
Hpy8I GTNNAC 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 438
HpyCH4III ACNGT 2 cut(s) 341, 388
HpyCH4IV ACGT 1 cut(s) 565
HpySE526I ACGT 1 cut(s) 565
Hsp92II CATG 4 cut(s) 29, 251, 268, 289
Kzo9I GATC 2 cut(s) 67, 352
LmnI GCTCC 1 cut(s) 417
LpnPI CCDG 6 cut(s) 78, 90, 312, 319, 335, 465
MaeI CTAG 2 cut(s) 165, 525
MaeII ACGT 1 cut(s) 565
MaeIII GTNAC 3 cut(s) 250, 382, 510
MalI GATC 2 cut(s) 69, 354
MboI GATC 2 cut(s) 67, 352
MboII GAAGA 6 cut(s) 196, 197, 239, 382, 489, 513
MluCI AATT 3 cut(s) 30, 255, 436
MnlI CCTC 4 cut(s) 72, 144, 323, 332
MseI TTAA 3 cut(s) 216, 315, 568
MspA1I CMGCKG 1 cut(s) 501
MspI CCGG 1 cut(s) 452
MvnI CGCG 1 cut(s) 357
NcoI CCATGG 1 cut(s) 285
NdeII GATC 2 cut(s) 67, 352
NlaIII CATG 4 cut(s) 29, 251, 268, 289
NlaIV GGNNCC 1 cut(s) 419
NmuCI GTSAC 2 cut(s) 250, 382
PfeI GAWTC 1 cut(s) 100
PshBI ATTAAT 1 cut(s) 315
Psp1406I AACGTT 1 cut(s) 565
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 2 cut(s) 503, 532
PsrI GAACNNNNNNTAC 1 cut(s) 38
RsaI GTAC 2 cut(s) 281, 491
RsaNI GTAC 2 cut(s) 280, 490
Rsr2I CGGWCCG 1 cut(s) 503
RsrII CGGWCCG 1 cut(s) 503
SaqAI TTAA 3 cut(s) 216, 315, 568
Sau3AI GATC 2 cut(s) 67, 352
Sau96I GGNCC 2 cut(s) 503, 532
SetI ASST 2 cut(s) 449, 568
SinI GGWCC 2 cut(s) 503, 532
Sse9I AATT 3 cut(s) 30, 255, 436
SsiI CCGC 3 cut(s) 357, 501, 506
SspMI CTAG 2 cut(s) 165, 525
StyI CCWWGG 2 cut(s) 238, 285
TaaI ACNGT 2 cut(s) 341, 388
TaiI ACGT 1 cut(s) 568
TaqI TCGA 2 cut(s) 173, 192
TaqII GACCGA 1 cut(s) 520
TasI AATT 3 cut(s) 30, 255, 436
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 3 cut(s) 216, 315, 568
Tru9I TTAA 3 cut(s) 216, 315, 568
TscAI CASTG 3 cut(s) 331, 393, 462
TseFI GTSAC 2 cut(s) 250, 382
Tsp45I GTSAC 2 cut(s) 250, 382
TspDTI ATGAA 2 cut(s) 98, 236
TspRI CASTG 3 cut(s) 331, 393, 462
VpaK11BI GGWCC 2 cut(s) 503, 532
VspI ATTAAT 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 237
XapI RAATTY 1 cut(s) 30
XmiI GTMKAC 1 cut(s) 88
XspI CTAG 2 cut(s) 165, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.