Rh2BG298900

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002989
Physical Location & Seq
Forward (+)
34212205 .. 34213221
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG298900.1

Sequence Viewer

Length: 810 bp
ATGTCTTCGAGTATGAGTTTGGGGAAGAGCTCTAGGGCACCCATGACTAGTCTTACCGATGAAAAAGATATCAAGGCCGCTTTTAGTCTTTGCTTCATGAAGCATGTGGTGCTTGATTCTGAAAAGGAGATACAGAAATTGGAGGACACGAAGCGCAAGTCTGTTGGCCATATTAGGGTATACCCGAATCCCGAAACCAAACCAAGGGCGCTGCAAATCGGAAAACAAGAGGAAGACACTGTCAATGTTGCACAAGGCTTGTCCAGAAAGCAGCAACTACAACAAGGTCCTATGCAGAAAGCGGTGCACACTGAAGAACAACAAGCTATGAATGGAGCAAAACCACAGCAAGGTTTGTTCTATGGAGACTATACCCCTCCTAATCTCCCACCAGTCCCAAGCCTACACAAATTGATCGAAATTTGCAGCGAGCCTTTTGAGAAGCAATTGACATGTAGTGATGTGAGGGATGACCAGTCAAGGCTAGCTATGAGCAAAGAAGATGTGCAAAAGCATCTAATGCCATTGTTGAATGAGAGTGAAGATCTCAACGAAGGAATTGACGTGACGACTTATGATTTGGCTGGTAAGGAGTACCCCATGAAGTTCAAAACTTGGGTGTCCAAGATTCATGTCTTAACTGGAGGATGGAAAGCTTTCTGCCGTGATCATCAATTGGTTCAGACACAACATTTTGTCACAGTGTGGATGTTTCGGAATATTGCAACCGGCAATCTTTGCTTCGTAATCGAGGTGAGAAGGTTGCCGGTGTTTGAAACCATCAAGAGAAGAAGAATACGGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

269

Amino Acids

30.81

Weight (kDa)

8.86

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 146 - 233 2e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 180
AciI CCGC 2 cut(s) 78, 302
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 1 cut(s) 420
AcuI CTGAAG 1 cut(s) 333
AdeI CACNNNGTG 1 cut(s) 705
AfaI GTAC 1 cut(s) 596
AfiI CCNNNNNNNGG 3 cut(s) 175, 204, 350
AflIII ACRYGT 1 cut(s) 452
AgsI TTSAA 3 cut(s) 532, 610, 776
AhlI ACTAGT 1 cut(s) 47
AjiI CACGTC 1 cut(s) 565
AluBI AGCT 4 cut(s) 30, 326, 488, 656
AluI AGCT 4 cut(s) 30, 326, 488, 656
Alw21I GWGCWC 2 cut(s) 32, 309
Alw26I GTCTC 1 cut(s) 360
Alw44I GTGCAC 1 cut(s) 305
AoxI GGCC 2 cut(s) 75, 166
ApaLI GTGCAC 1 cut(s) 305
ApeKI GCWGC 3 cut(s) 211, 271, 426
ApoI RAATTY 1 cut(s) 420
ArsI GACNNNNNNTTYG 4 cut(s) 143, 175, 562, 594
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 2 cut(s) 156, 211
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 766
AsuNHI GCTAGC 1 cut(s) 484
AvaII GGWCC 1 cut(s) 287
BaeGI GKGCMC 2 cut(s) 40, 309
BalI TGGCCA 1 cut(s) 168
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 2 cut(s) 32, 309
BbvI GCAGC 3 cut(s) 198, 283, 438
BccI CCATC 2 cut(s) 642, 788
BceAI ACGGC 1 cut(s) 648
BclI TGATCA 1 cut(s) 667
BcoDI GTCTC 1 cut(s) 360
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 3 cut(s) 33, 48, 485
BfoI RGCGCY 1 cut(s) 212
BglII AGATCT 1 cut(s) 544
BisI GCNGC 4 cut(s) 78, 212, 272, 427
BlsI GCNGC 4 cut(s) 79, 213, 273, 428
Bme18I GGWCC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 39
BmsI GCATC 1 cut(s) 523
BmtI GCTAGC 1 cut(s) 488
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 663
BsaJI CCNNGG 1 cut(s) 203
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 204, 350
Bse118I RCCGGY 2 cut(s) 728, 766
Bse1I ACTGG 3 cut(s) 392, 475, 646
BseDI CCNNGG 1 cut(s) 203
BseGI GGATG 3 cut(s) 475, 653, 714
BseLI CCNNNNNNNGG 3 cut(s) 175, 204, 350
BseNI ACTGG 3 cut(s) 392, 475, 646
BseSI GKGCMC 2 cut(s) 40, 309
BseXI GCAGC 3 cut(s) 198, 283, 438
BshFI GGCC 2 cut(s) 77, 168
BshNI GGYRCC 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 32, 309
BsiSI CCGG 2 cut(s) 729, 767
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 3 cut(s) 175, 204, 350
BsmAI GTCTC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 2 cut(s) 77, 168
Bsp1286I GDGCHC 3 cut(s) 32, 40, 309
Bsp143I GATC 3 cut(s) 414, 544, 667
BspACI CCGC 2 cut(s) 78, 302
BspANI GGCC 2 cut(s) 77, 168
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 1 cut(s) 39
BspOI GCTAGC 1 cut(s) 488
BspQI GCTCTTC 1 cut(s) 20
BspT107I GGYRCC 1 cut(s) 37
BsrFI RCCGGY 2 cut(s) 728, 766
BsrI ACTGG 3 cut(s) 392, 475, 646
BssAI RCCGGY 2 cut(s) 728, 766
BssECI CCNNGG 1 cut(s) 203
BssMI GATC 3 cut(s) 414, 544, 667
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 203
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 2 cut(s) 241, 703
Bst6I CTCTTC 1 cut(s) 20
BstAPI GCANNNNNTGC 3 cut(s) 109, 520, 738
BstC8I GCNNGC 2 cut(s) 431, 486
BstF5I GGATG 3 cut(s) 475, 653, 714
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 2 cut(s) 156, 211
BstKTI GATC 3 cut(s) 417, 547, 670
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 3 cut(s) 414, 544, 667
BstMWI GCNNNNNNNGC 3 cut(s) 109, 520, 738
BstNSI RCATGY 2 cut(s) 107, 456
BstSLI GKGCMC 2 cut(s) 40, 309
BstV1I GCAGC 3 cut(s) 198, 283, 438
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BstZ17I GTATAC 1 cut(s) 181
BsuRI GGCC 2 cut(s) 77, 168
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 3 cut(s) 475, 653, 714
BtsIMutI CAGTG 3 cut(s) 237, 309, 708
Cac8I GCNNGC 2 cut(s) 431, 486
CciI TCATGA 1 cut(s) 96
CfoI GCGC 2 cut(s) 156, 211
Cfr10I RCCGGY 2 cut(s) 728, 766
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 595
CviAII CATG 6 cut(s) 43, 97, 104, 453, 601, 632
CviQI GTAC 1 cut(s) 595
DpnI GATC 3 cut(s) 416, 546, 669
DpnII GATC 3 cut(s) 414, 544, 667
DraIII CACNNNGTG 1 cut(s) 705
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 287
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 287
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 203
EcoT38I GRGCYC 1 cut(s) 32
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 6 cut(s) 46, 100, 107, 456, 604, 635
FaqI GGGAC 1 cut(s) 380
FatI CATG 6 cut(s) 42, 96, 103, 452, 600, 631
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 78, 212, 272, 427
FokI GGATG 3 cut(s) 482, 660, 721
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 4 cut(s) 78, 212, 272, 427
FspBI CTAG 3 cut(s) 33, 48, 485
GlaI GCGC 2 cut(s) 155, 210
GluI GCNGC 4 cut(s) 78, 212, 272, 427
GsuI CTGGAG 1 cut(s) 663
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 2 cut(s) 77, 168
HapII CCGG 2 cut(s) 729, 767
HhaI GCGC 2 cut(s) 156, 211
Hin1II CATG 6 cut(s) 46, 100, 107, 456, 604, 635
Hin6I GCGC 2 cut(s) 154, 209
HinP1I GCGC 2 cut(s) 154, 209
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 3 cut(s) 116, 187, 628
HpaII CCGG 2 cut(s) 729, 767
HphI GGTGA 1 cut(s) 766
Hpy166II GTNNAC 2 cut(s) 181, 307
Hpy188I TCNGA 4 cut(s) 121, 221, 684, 717
Hpy188III TCNNGA 4 cut(s) 97, 191, 264, 784
Hpy8I GTNNAC 2 cut(s) 181, 307
HpyAV CCTTC 2 cut(s) 548, 753
HpyCH4III ACNGT 2 cut(s) 241, 703
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 7 cut(s) 214, 251, 295, 307, 426, 508, 725
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 520, 738
HpySE526I ACGT 1 cut(s) 564
Hsp92II CATG 6 cut(s) 46, 100, 107, 456, 604, 635
HspAI GCGC 2 cut(s) 154, 209
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 3 cut(s) 414, 544, 667
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 1 cut(s) 335
LpnPI CCDG 7 cut(s) 277, 405, 488, 570, 627, 742, 780
Lsp1109I GCAGC 3 cut(s) 198, 283, 438
LweI GCATC 1 cut(s) 523
MaeI CTAG 3 cut(s) 33, 48, 485
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 565, 697
MalI GATC 3 cut(s) 416, 546, 669
MboI GATC 3 cut(s) 414, 544, 667
MboII GAAGA 7 cut(s) 37, 245, 326, 512, 554, 801, 804
MfeI CAATTG 2 cut(s) 446, 674
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 3 cut(s) 32, 40, 309
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 7 cut(s) 137, 410, 420, 446, 558, 674, 805
MluNI TGGCCA 1 cut(s) 168
MnlI CCTC 6 cut(s) 136, 223, 387, 459, 638, 745
Mox20I TGGCCA 1 cut(s) 168
MroXI GAANNNNTTC 1 cut(s) 656
MscI TGGCCA 1 cut(s) 168
MseI TTAA 1 cut(s) 638
Msp20I TGGCCA 1 cut(s) 168
MspI CCGG 2 cut(s) 729, 767
MunI CAATTG 2 cut(s) 446, 674
MwoI GCNNNNNNNGC 3 cut(s) 109, 520, 738
NdeII GATC 3 cut(s) 414, 544, 667
NheI GCTAGC 1 cut(s) 484
NlaIII CATG 6 cut(s) 46, 100, 107, 456, 604, 635
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 565, 697
NspI RCATGY 2 cut(s) 107, 456
PagI TCATGA 1 cut(s) 96
PciI ACATGT 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 20
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 116, 187, 628
PflFI GACNNNGTC 1 cut(s) 239
PkrI GCNGC 4 cut(s) 79, 213, 273, 428
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 1 cut(s) 452
Psp124BI GAGCTC 1 cut(s) 32
Psp5II RGGWCCY 1 cut(s) 287
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 287
PspPPI RGGWCCY 1 cut(s) 287
PsuI RGATCY 1 cut(s) 544
PsyI GACNNNGTC 1 cut(s) 239
RsaI GTAC 1 cut(s) 596
RsaNI GTAC 1 cut(s) 595
SacI GAGCTC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 1 cut(s) 638
SatI GCNGC 4 cut(s) 78, 212, 272, 427
Sau3AI GATC 3 cut(s) 414, 544, 667
Sau96I GGNCC 1 cut(s) 287
SduI GDGCHC 3 cut(s) 32, 40, 309
SetI ASST 9 cut(s) 32, 289, 328, 355, 490, 567, 658, 756, 764
SfaNI GCATC 1 cut(s) 523
SinI GGWCC 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 7 cut(s) 137, 410, 420, 446, 558, 674, 805
SsiI CCGC 2 cut(s) 78, 302
SspI AATATT 1 cut(s) 721
SspMI CTAG 3 cut(s) 33, 48, 485
SstI GAGCTC 1 cut(s) 32
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 2 cut(s) 241, 703
TaiI ACGT 1 cut(s) 567
TaqI TCGA 3 cut(s) 8, 417, 750
TasI AATT 7 cut(s) 137, 410, 420, 446, 558, 674, 805
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 3 cut(s) 116, 187, 628
Tru1I TTAA 1 cut(s) 638
Tru9I TTAA 1 cut(s) 638
TscAI CASTG 3 cut(s) 244, 316, 708
TseFI GTSAC 2 cut(s) 565, 697
TseI GCWGC 3 cut(s) 211, 271, 426
Tsp45I GTSAC 2 cut(s) 565, 697
TspDTI ATGAA 6 cut(s) 75, 85, 113, 344, 617, 620
TspRI CASTG 3 cut(s) 244, 316, 708
Tth111I GACNNNGTC 1 cut(s) 239
VneI GTGCAC 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 1 cut(s) 420
XceI RCATGY 2 cut(s) 107, 456
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 3 cut(s) 33, 48, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.