Rw7G010980

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002989
Physical Location & Seq
Reverse (-)
10218847 .. 10219679
833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G010980.1

Sequence Viewer

Length: 789 bp
ATGGCTAGGCTGACCAATGAAGAAGACGATGATATGATGAAGGCCGTTTTTAATCTTTGTCATATGAAGCATGTGCTTGTTGATTCTACAAAAGACATACAGATATTGGAGGATACTAACCGCAAGTGTCTTAGTCAAATTAATGAATGCATTGTTAATGTTGCCCAAGGCTTGCATAGAAACCAACGACTACAACAGGATCCTAAGAGAAAAGCTTTGCAAGTCACAGGACAAGAAGCCATTTATGCTGGACAAGGCTTAGCAAACTCAAACGTATGTCAAGGTTTGTTCTATGGAAACTATGCCCCTCCTAATCTCCCACCAGTCCCACGCCTACGACAATTTATTGAAATTTGCAGTCAGCCGTTTGAGAAGCAATTAACATTTAGTGATGTGAAGGATGACCAATCAAGGCTAGCTATGAGCAAAGAGGATGTCCAAAAGCGCCTAATGCCATTGTTGAATGAGAATGAAGATCTTAGACGAGGAATCCAAGTCACAACTTATGATTTGGATGGTAAAGAGTATCCCATGGTGTTCAAAATTTGGGTGGAAAAGATTCATGTCTTGACCGGAGGATGGAAAGCTTTTTGCCATGATCATAAGCTGGTTGAGAAGCAAGATTTTGTGACCGTATGGATGTTTCGAAATGCTGCAACCGGTAGCCTTTGCTTTGTAATTGATTCGAGAAGGCTGCCAGTGCTTGAAACCATCAAGAGAAGAAGAACAAGGCGAAATCGAGATATGCAGATCACCACCATGAATCGAGAAAGGTCTAATAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

262

Amino Acids

30.51

Weight (kDa)

8.94

Isoelectric Point (pI)

63.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 123 - 224 1e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 121
AclWI GGATC 2 cut(s) 194, 207
AcsI RAATTY 2 cut(s) 351, 543
AfiI CCNNNNNNNGG 1 cut(s) 579
AgeI ACCGGT 1 cut(s) 659
AgsI TTSAA 4 cut(s) 350, 463, 541, 707
AluBI AGCT 4 cut(s) 215, 419, 587, 607
AluI AGCT 4 cut(s) 215, 419, 587, 607
AlwI GGATC 2 cut(s) 194, 207
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 2 cut(s) 653, 694
ApoI RAATTY 2 cut(s) 351, 543
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AseI ATTAAT 1 cut(s) 141
AsiGI ACCGGT 1 cut(s) 659
Asp700I GAANNNNTTC 1 cut(s) 558
AspLEI GCGC 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 745
AsuII TTCGAA 1 cut(s) 646
AsuNHI GCTAGC 1 cut(s) 415
BamHI GGATCC 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 640, 681
BccI CCATC 3 cut(s) 509, 573, 719
BceAI ACGGC 2 cut(s) 29, 349
BcgI CGANNNNNNTGC 2 cut(s) 676, 710
BciVI GTATCC 2 cut(s) 106, 537
BclI TGATCA 1 cut(s) 598
BfaI CTAG 2 cut(s) 6, 416
BfoI RGCGCY 1 cut(s) 448
BfuI GTATCC 2 cut(s) 106, 537
BglII AGATCT 1 cut(s) 475
BisI GCNGC 2 cut(s) 654, 695
BlpI GCTNAGC 1 cut(s) 259
BlsI GCNGC 2 cut(s) 655, 696
BmiI GGNNCC 1 cut(s) 201
BmtI GCTAGC 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 30
Bpu1102I GCTNAGC 1 cut(s) 259
Bpu14I TTCGAA 1 cut(s) 646
BsaJI CCNNGG 2 cut(s) 166, 531
BsaWI WCCGGW 2 cut(s) 572, 659
Bsc4I CCNNNNNNNGG 1 cut(s) 579
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 2 cut(s) 323, 698
BseDI CCNNGG 2 cut(s) 166, 531
BseGI GGATG 5 cut(s) 406, 439, 520, 584, 645
BseLI CCNNNNNNNGG 1 cut(s) 579
BseNI ACTGG 2 cut(s) 323, 698
BseXI GCAGC 2 cut(s) 640, 681
BshFI GGCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 659
BsiSI CCGG 2 cut(s) 573, 660
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 579
BsmFI GGGAC 1 cut(s) 311
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 1 cut(s) 44
Bsp119I TTCGAA 1 cut(s) 646
Bsp143I GATC 4 cut(s) 199, 475, 598, 750
Bsp1720I GCTNAGC 1 cut(s) 259
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 1 cut(s) 121
BspANI GGCC 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 201
BspOI GCTAGC 1 cut(s) 419
BspPI GGATC 2 cut(s) 194, 207
BspT104I TTCGAA 1 cut(s) 646
BsrFI RCCGGY 1 cut(s) 659
BsrI ACTGG 2 cut(s) 323, 698
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 2 cut(s) 166, 531
BssMI GATC 4 cut(s) 199, 475, 598, 750
BssT1I CCWWGG 2 cut(s) 166, 531
Bst4CI ACNGT 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 646
BstC8I GCNNGC 2 cut(s) 173, 417
BstDEI CTNAG 4 cut(s) 131, 204, 259, 479
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 5 cut(s) 406, 439, 520, 584, 645
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 4 cut(s) 202, 478, 601, 753
BstMBI GATC 4 cut(s) 199, 475, 598, 750
BstMWI GCNNNNNNNGC 3 cut(s) 245, 451, 700
BstNSI RCATGY 1 cut(s) 74
BstV1I GCAGC 2 cut(s) 640, 681
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 199, 475
BstYI RGATCY 2 cut(s) 199, 475
BsuI GTATCC 2 cut(s) 106, 537
BsuRI GGCC 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 5 cut(s) 406, 439, 520, 584, 645
BtsIMutI CAGTG 2 cut(s) 705, 784
Cac8I GCNNGC 2 cut(s) 173, 417
CfoI GCGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 659
CspAI ACCGGT 1 cut(s) 659
CviAII CATG 5 cut(s) 71, 532, 563, 596, 760
DdeI CTNAG 4 cut(s) 131, 204, 259, 479
DpnI GATC 4 cut(s) 201, 477, 600, 752
DpnII GATC 4 cut(s) 199, 475, 598, 750
Eco130I CCWWGG 2 cut(s) 166, 531
EcoT14I CCWWGG 2 cut(s) 166, 531
EcoT22I ATGCAT 1 cut(s) 152
ErhI CCWWGG 2 cut(s) 166, 531
FaeI CATG 5 cut(s) 74, 535, 566, 599, 763
FaqI GGGAC 1 cut(s) 311
FatI CATG 5 cut(s) 70, 531, 562, 595, 759
FauNDI CATATG 1 cut(s) 63
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 2 cut(s) 654, 695
FokI GGATG 5 cut(s) 413, 446, 527, 591, 652
Fsp4HI GCNGC 2 cut(s) 654, 695
FspBI CTAG 2 cut(s) 6, 416
GlaI GCGC 1 cut(s) 446
GluI GCNGC 2 cut(s) 654, 695
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 1 cut(s) 44
HapII CCGG 2 cut(s) 573, 660
HhaI GCGC 1 cut(s) 447
Hin1II CATG 5 cut(s) 74, 535, 566, 599, 763
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HindIII AAGCTT 2 cut(s) 213, 585
HinfI GANTC 5 cut(s) 83, 489, 559, 683, 763
HpaII CCGG 2 cut(s) 573, 660
HphI GGTGA 1 cut(s) 745
Hpy188III TCNNGA 5 cut(s) 568, 687, 715, 740, 767
HpyAV CCTTC 3 cut(s) 34, 391, 684
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 6 cut(s) 150, 175, 220, 357, 656, 748
HpyF10VI GCNNNNNNNGC 3 cut(s) 245, 451, 700
HpyF3I CTNAG 4 cut(s) 131, 204, 259, 479
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 5 cut(s) 74, 535, 566, 599, 763
HspAI GCGC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 4 cut(s) 199, 475, 598, 750
LpnPI CCDG 8 cut(s) 182, 213, 234, 336, 586, 593, 673, 711
Lsp1109I GCAGC 2 cut(s) 640, 681
MaeI CTAG 2 cut(s) 6, 416
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 4 cut(s) 223, 496, 628, 782
MalI GATC 4 cut(s) 201, 477, 600, 752
MboI GATC 4 cut(s) 199, 475, 598, 750
MboII GAAGA 5 cut(s) 32, 35, 485, 732, 735
MflI RGATCY 2 cut(s) 199, 475
MluCI AATT 6 cut(s) 138, 341, 351, 377, 543, 678
MnlI CCTC 5 cut(s) 103, 318, 424, 479, 569
Mph1103I ATGCAT 1 cut(s) 152
MroXI GAANNNNTTC 1 cut(s) 558
MseI TTAA 4 cut(s) 51, 141, 156, 380
MslI CAYNNNNRTG 1 cut(s) 758
MspI CCGG 2 cut(s) 573, 660
Mva1269I GAATGC 1 cut(s) 152
MwoI GCNNNNNNNGC 3 cut(s) 245, 451, 700
NcoI CCATGG 1 cut(s) 531
NdeI CATATG 1 cut(s) 63
NdeII GATC 4 cut(s) 199, 475, 598, 750
NheI GCTAGC 1 cut(s) 415
NlaIII CATG 5 cut(s) 74, 535, 566, 599, 763
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 4 cut(s) 223, 496, 628, 782
NsiI ATGCAT 1 cut(s) 152
NspI RCATGY 1 cut(s) 74
NspV TTCGAA 1 cut(s) 646
PctI GAATGC 1 cut(s) 152
PdmI GAANNNNTTC 1 cut(s) 558
PfeI GAWTC 5 cut(s) 83, 489, 559, 683, 763
PinAI ACCGGT 1 cut(s) 659
PkrI GCNGC 2 cut(s) 655, 696
PshBI ATTAAT 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 201
PsuI RGATCY 2 cut(s) 199, 475
RseI CAYNNNNRTG 1 cut(s) 758
SaqAI TTAA 4 cut(s) 51, 141, 156, 380
SatI GCNGC 2 cut(s) 654, 695
Sau3AI GATC 4 cut(s) 199, 475, 598, 750
SetI ASST 7 cut(s) 217, 276, 286, 421, 589, 609, 776
SfuI TTCGAA 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 758
Sse9I AATT 6 cut(s) 138, 341, 351, 377, 543, 678
SsiI CCGC 1 cut(s) 121
SspMI CTAG 2 cut(s) 6, 416
StyI CCWWGG 2 cut(s) 166, 531
TaaI ACNGT 1 cut(s) 634
TaiI ACGT 1 cut(s) 276
TaqI TCGA 4 cut(s) 646, 686, 739, 766
TasI AATT 6 cut(s) 138, 341, 351, 377, 543, 678
TfiI GAWTC 5 cut(s) 83, 489, 559, 683, 763
Tru1I TTAA 4 cut(s) 51, 141, 156, 380
Tru9I TTAA 4 cut(s) 51, 141, 156, 380
TscAI CASTG 1 cut(s) 705
TseFI GTSAC 4 cut(s) 223, 496, 628, 782
TseI GCWGC 2 cut(s) 653, 694
Tsp45I GTSAC 4 cut(s) 223, 496, 628, 782
TspDTI ATGAA 7 cut(s) 33, 53, 80, 159, 486, 551, 776
TspRI CASTG 1 cut(s) 705
VspI ATTAAT 1 cut(s) 141
XapI RAATTY 2 cut(s) 351, 543
XceI RCATGY 1 cut(s) 74
XmnI GAANNNNTTC 1 cut(s) 558
XspI CTAG 2 cut(s) 6, 416
Zsp2I ATGCAT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.