RLG00000018577

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002989
Physical Location & Seq
Forward (+)
32132685 .. 32133482
798 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018577

Sequence Viewer

Length: 798 bp
ATGAGTTTGGAGAAGAGCTCTGGGGCACCCATAACTAGTCTTACCGATGAAAAAGATATCAAGGCCGCTTTTAGTCTTTGTTTTATGAAGCATGTGGTGCTTGATTCTGAAAAAGAGATACAGAAATTGGAGGACACGAAGCGCAAGTCTGTTGGCCATATTAGGGTATACCCGAATCCCGAAACCAAGCCAAGGGCACTGCAAGTCGAAAAACAAGAGGAAGACATTGTCAATGTTGCACAAGGCTTGTCCAGAAAGCAGCAACTACAACAAGGTCCTAAGCGGAAAGCGGTGCACGCTGAAGAACAAGAAGCTATGAATGGAGCAAAACCACAGCAAGGTTTGTTCTATGGAGACTATACCCCTCCTAATCTCCCACCAGTTCCAAGCCTACACAAATTGATCGAAATTTGCAGCGAGCCTTTTGAGAAGCAATTGACATGTAGTGATGTGAGGGATGACCAGTCAAGGCTAGCTATGAGCAAAGAAGACGTGCAAAAGCATCTAATGCCATTGTTGAATGAAAGTGAAGATCTTAACGAAGGAATTGATGTGACGACTTATGATTTGGCTGGTAAGGAGTACCCCATGAAGTTCAAAACTTGGGTGTCCAAGATTCATGTCTTAACTGGAGGATGGAAAGCTTTCTGCCGTGATCATCAATTGGTTCAGACACAACATTTTGTAACAGTGTGGATGTTTCGGAATATCGCAACCGGCAATCTTTGCTTCGTAATCGAGGTGAGAAGGTTGCCGGTGTTTGAAACCATCAAGAGAAGAAGAATAAGGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

266

Amino Acids

30.44

Weight (kDa)

8.49

Isoelectric Point (pI)

61.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 142 - 229 2e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 168
AciI CCGC 3 cut(s) 66, 283, 290
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 1 cut(s) 408
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 1 cut(s) 584
AfiI CCNNNNNNNGG 3 cut(s) 163, 192, 338
AflIII ACRYGT 1 cut(s) 440
AgsI TTSAA 3 cut(s) 520, 598, 764
AhlI ACTAGT 1 cut(s) 35
AjiI CACGTC 1 cut(s) 493
AluBI AGCT 4 cut(s) 18, 314, 476, 644
AluI AGCT 4 cut(s) 18, 314, 476, 644
Alw21I GWGCWC 2 cut(s) 20, 297
Alw26I GTCTC 1 cut(s) 348
Alw44I GTGCAC 1 cut(s) 293
AoxI GGCC 2 cut(s) 63, 154
ApaLI GTGCAC 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 259, 414
ApoI RAATTY 1 cut(s) 408
ArsI GACNNNNNNTTYG 4 cut(s) 131, 163, 550, 582
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 1 cut(s) 754
AsuNHI GCTAGC 1 cut(s) 472
AvaII GGWCC 1 cut(s) 275
BaeGI GKGCMC 3 cut(s) 28, 199, 297
BalI TGGCCA 1 cut(s) 156
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 2 cut(s) 228, 495
Bbv12I GWGCWC 2 cut(s) 20, 297
BbvI GCAGC 2 cut(s) 271, 426
BccI CCATC 2 cut(s) 630, 776
BceAI ACGGC 1 cut(s) 636
BclI TGATCA 1 cut(s) 655
BcoDI GTCTC 1 cut(s) 348
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 2 cut(s) 36, 473
BglII AGATCT 1 cut(s) 532
BisI GCNGC 3 cut(s) 66, 260, 415
BlsI GCNGC 3 cut(s) 67, 261, 416
Bme18I GGWCC 1 cut(s) 275
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 27
BmsI GCATC 1 cut(s) 511
BmtI GCTAGC 1 cut(s) 476
BpiI GAAGAC 2 cut(s) 228, 495
BplI GAGNNNNNCTC 1 cut(s) 34
BpmI CTGGAG 1 cut(s) 651
Bpu10I CCTNAGC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 191
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 192, 338
Bse118I RCCGGY 2 cut(s) 716, 754
Bse1I ACTGG 3 cut(s) 380, 463, 634
BseDI CCNNGG 1 cut(s) 191
BseGI GGATG 3 cut(s) 463, 641, 702
BseLI CCNNNNNNNGG 3 cut(s) 163, 192, 338
BseNI ACTGG 3 cut(s) 380, 463, 634
BseSI GKGCMC 3 cut(s) 28, 199, 297
BseXI GCAGC 2 cut(s) 271, 426
BshFI GGCC 2 cut(s) 65, 156
BshNI GGYRCC 1 cut(s) 25
BsiHKAI GWGCWC 2 cut(s) 20, 297
BsiSI CCGG 2 cut(s) 717, 755
BslI CCNNNNNNNGG 3 cut(s) 163, 192, 338
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 2 cut(s) 65, 156
Bsp1286I GDGCHC 4 cut(s) 20, 28, 199, 297
Bsp143I GATC 3 cut(s) 402, 532, 655
BspACI CCGC 3 cut(s) 66, 283, 290
BspANI GGCC 2 cut(s) 65, 156
BspLI GGNNCC 1 cut(s) 27
BspOI GCTAGC 1 cut(s) 476
BspQI GCTCTTC 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 25
BsrFI RCCGGY 2 cut(s) 716, 754
BsrI ACTGG 3 cut(s) 380, 463, 634
BssAI RCCGGY 2 cut(s) 716, 754
BssECI CCNNGG 1 cut(s) 191
BssMI GATC 3 cut(s) 402, 532, 655
BssNAI GTATAC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 191
Bst1107I GTATAC 1 cut(s) 169
Bst4CI ACNGT 1 cut(s) 691
Bst6I CTCTTC 1 cut(s) 8
BstAPI GCANNNNNTGC 3 cut(s) 97, 508, 726
BstC8I GCNNGC 3 cut(s) 297, 419, 474
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 463, 641, 702
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 3 cut(s) 405, 535, 658
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 3 cut(s) 402, 532, 655
BstMWI GCNNNNNNNGC 4 cut(s) 97, 296, 508, 726
BstNSI RCATGY 2 cut(s) 95, 444
BstSLI GKGCMC 3 cut(s) 28, 199, 297
BstV1I GCAGC 2 cut(s) 271, 426
BstV2I GAAGAC 2 cut(s) 228, 495
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BstZ17I GTATAC 1 cut(s) 169
BsuRI GGCC 2 cut(s) 65, 156
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 3 cut(s) 463, 641, 702
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 2 cut(s) 197, 696
Cac8I GCNNGC 3 cut(s) 297, 419, 474
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 2 cut(s) 716, 754
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 583
CviAII CATG 4 cut(s) 92, 441, 589, 620
CviQI GTAC 1 cut(s) 583
DdeI CTNAG 1 cut(s) 279
DpnI GATC 3 cut(s) 404, 534, 657
DpnII GATC 3 cut(s) 402, 532, 655
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 1 cut(s) 275
EcoRV GATATC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 191
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 4 cut(s) 95, 444, 592, 623
FatI CATG 4 cut(s) 91, 440, 588, 619
FbaI TGATCA 1 cut(s) 655
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 66, 260, 415
FokI GGATG 3 cut(s) 470, 648, 709
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 66, 260, 415
FspBI CTAG 2 cut(s) 36, 473
GlaI GCGC 1 cut(s) 143
GluI GCNGC 3 cut(s) 66, 260, 415
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 2 cut(s) 65, 156
HapII CCGG 2 cut(s) 717, 755
HhaI GCGC 1 cut(s) 144
Hin1II CATG 4 cut(s) 95, 444, 592, 623
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 3 cut(s) 104, 175, 616
HpaII CCGG 2 cut(s) 717, 755
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 2 cut(s) 169, 295
Hpy188I TCNGA 3 cut(s) 109, 672, 705
Hpy188III TCNNGA 3 cut(s) 179, 252, 772
Hpy8I GTNNAC 2 cut(s) 169, 295
HpyAV CCTTC 2 cut(s) 536, 741
HpyCH4III ACNGT 1 cut(s) 691
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 5 cut(s) 202, 239, 295, 414, 496
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 296, 508, 726
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 4 cut(s) 95, 444, 592, 623
HspAI GCGC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 655
Kzo9I GATC 3 cut(s) 402, 532, 655
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 8 cut(s) 6, 265, 393, 476, 558, 615, 730, 768
Lsp1109I GCAGC 2 cut(s) 271, 426
LweI GCATC 1 cut(s) 511
MaeI CTAG 2 cut(s) 36, 473
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 2 cut(s) 553, 685
MalI GATC 3 cut(s) 404, 534, 657
MboI GATC 3 cut(s) 402, 532, 655
MboII GAAGA 7 cut(s) 25, 233, 314, 500, 542, 789, 792
MfeI CAATTG 2 cut(s) 434, 662
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 4 cut(s) 20, 28, 199, 297
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 7 cut(s) 125, 398, 408, 434, 546, 662, 793
MluNI TGGCCA 1 cut(s) 156
MnlI CCTC 6 cut(s) 124, 211, 375, 447, 626, 733
Mox20I TGGCCA 1 cut(s) 156
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 156
MseI TTAA 2 cut(s) 537, 626
Msp20I TGGCCA 1 cut(s) 156
MspI CCGG 2 cut(s) 717, 755
MunI CAATTG 2 cut(s) 434, 662
MwoI GCNNNNNNNGC 4 cut(s) 97, 296, 508, 726
NdeII GATC 3 cut(s) 402, 532, 655
NheI GCTAGC 1 cut(s) 472
NlaIII CATG 4 cut(s) 95, 444, 592, 623
NlaIV GGNNCC 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 553
NspI RCATGY 2 cut(s) 95, 444
PciI ACATGT 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 8
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 104, 175, 616
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 3 cut(s) 67, 261, 416
PpuMI RGGWCCY 1 cut(s) 275
PscI ACATGT 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 275
PspN4I GGNNCC 1 cut(s) 27
PspPI GGNCC 1 cut(s) 275
PspPPI RGGWCCY 1 cut(s) 275
PsuI RGATCY 1 cut(s) 532
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 1 cut(s) 584
RsaNI GTAC 1 cut(s) 583
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 537, 626
SatI GCNGC 3 cut(s) 66, 260, 415
Sau3AI GATC 3 cut(s) 402, 532, 655
Sau96I GGNCC 1 cut(s) 275
SduI GDGCHC 4 cut(s) 20, 28, 199, 297
SetI ASST 9 cut(s) 20, 277, 316, 343, 478, 495, 646, 744, 752
SfaNI GCATC 1 cut(s) 511
SinI GGWCC 1 cut(s) 275
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 7 cut(s) 125, 398, 408, 434, 546, 662, 793
SsiI CCGC 3 cut(s) 66, 283, 290
SspMI CTAG 2 cut(s) 36, 473
SstI GAGCTC 1 cut(s) 20
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 1 cut(s) 691
TaiI ACGT 1 cut(s) 495
TaqI TCGA 3 cut(s) 207, 405, 738
TasI AATT 7 cut(s) 125, 398, 408, 434, 546, 662, 793
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 104, 175, 616
Tru1I TTAA 2 cut(s) 537, 626
Tru9I TTAA 2 cut(s) 537, 626
TscAI CASTG 2 cut(s) 204, 696
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 2 cut(s) 259, 414
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 6 cut(s) 63, 101, 332, 537, 605, 608
TspRI CASTG 2 cut(s) 204, 696
Tth111I GACNNNGTC 1 cut(s) 227
VneI GTGCAC 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 1 cut(s) 408
XceI RCATGY 2 cut(s) 95, 444
XmiI GTMKAC 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 2 cut(s) 36, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.