MD02G1059300.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002989
Physical Location & Seq
Reverse (-)
4763138 .. 4763923
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1059300.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGATGATCGAGACCATGAAGAAGACCCTGATTGTAAAGACAAATCGATGACGATGAAGGAAGGTGCTTTAATTCTTATACATATGAAGCATAAGGTTTTGGATTCCGATAGTGCGAGGGAATTGGAAAATATGAAATGCAAATTTCTTGGTTCAAATTATTGGAAGAAGAAATACTCACATGAAGGAACGACGAGGAAACGTATAATTCAAGAAACTGAAGCCCCAAAAACCCTAGAAGCCGTTTATAATGTTGCTGCTGCACAAGGACAACAACCCTTGACTGGAGAGTTACAAAAACTAAAAGACGAGGAAGTTGAAGCAGAAGGTTTGTTCTATGGGAACTATGCCCCTCCTGATGTGCCACATGTTCCTAGAATACAGCACTTAATTCAAGTGTGCAGCAAGCCCTTTGAGAAACAACTCACAAGTAGTGATGTGAGGGATGGCCAGTGCAGGTTCGCCATCAACAAAGAAGACGTCGAAAACCATATCTTCCTGTTGTTGAAGAAAGGTGAAGACATTAGGAAGGGCATCCATGTCACAACTTATGATGTGGACGGTAACGATTACCCGATGGTATACAAACTTTGGGCGGGTAAAATTCATGTCCTCACTGGAGGCTGGAAAAACTTCGTTCATGATCATGGACTCGTTGAGAAGCAAGATTTTGTGACGTTGTGGGTTTTCCGGAATGCTGATAATGGAAGCCTTTGCTTTGTGATTAGTTCAAGAAGGCTGCCGGTGTATGAAACCATCAAGAAAAGAAGATTGAGGAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

262

Amino Acids

30.15

Weight (kDa)

7.17

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 138 - 245 2.7e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AatII GACGTC 1 cut(s) 483
Acc36I ACCTGC 1 cut(s) 447
AccI GTMKAC 1 cut(s) 582
AccIII TCCGGA 1 cut(s) 690
AciI CCGC 1 cut(s) 596
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 2 cut(s) 143, 603
AcuI CTGAAG 1 cut(s) 240
AcyI GRCGYC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 6 cut(s) 156, 212, 320, 395, 508, 732
Alw26I GTCTC 1 cut(s) 6
Aor13HI TCCGGA 1 cut(s) 690
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 4 cut(s) 257, 260, 402, 739
ApoI RAATTY 2 cut(s) 143, 603
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
Asp700I GAANNNNTTC 1 cut(s) 632
AsuHPI GGTGA 1 cut(s) 527
BalI TGGCCA 1 cut(s) 450
BarI GAAGNNNNNNTAC 2 cut(s) 158, 190
BbsI GAAGAC 3 cut(s) 30, 483, 525
BbvI GCAGC 4 cut(s) 244, 247, 414, 726
BccI CCATC 4 cut(s) 440, 473, 571, 764
BceAI ACGGC 1 cut(s) 227
BclI TGATCA 1 cut(s) 643
BcoDI GTCTC 1 cut(s) 6
BfaI CTAG 2 cut(s) 236, 375
BfuAI ACCTGC 1 cut(s) 447
BisI GCNGC 4 cut(s) 258, 261, 403, 740
BlsI GCNGC 4 cut(s) 259, 262, 404, 741
BmsI GCATC 1 cut(s) 543
BpiI GAAGAC 3 cut(s) 30, 483, 525
BpmI CTGGAG 2 cut(s) 306, 639
Bsa29I ATCGAT 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 6
BsaWI WCCGGW 1 cut(s) 690
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 742
Bse1I ACTGG 3 cut(s) 289, 451, 622
BseAI TCCGGA 1 cut(s) 690
BseCI ATCGAT 1 cut(s) 47
BseGI GGATG 3 cut(s) 10, 451, 534
BseLI CCNNNNNNNGG 1 cut(s) 284
BseNI ACTGG 3 cut(s) 289, 451, 622
BseXI GCAGC 4 cut(s) 244, 247, 414, 726
BsgI GTGCAG 3 cut(s) 246, 421, 475
BshFI GGCC 1 cut(s) 450
BshVI ATCGAT 1 cut(s) 47
BsiSI CCGG 2 cut(s) 691, 743
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 6
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 1 cut(s) 450
Bso31I GGTCTC 1 cut(s) 6
Bsp13I TCCGGA 1 cut(s) 690
Bsp143I GATC 2 cut(s) 7, 643
BspACI CCGC 1 cut(s) 596
BspANI GGCC 1 cut(s) 450
BspDI ATCGAT 1 cut(s) 47
BspEI TCCGGA 1 cut(s) 690
BspHI TCATGA 1 cut(s) 640
BspMI ACCTGC 1 cut(s) 447
BspTNI GGTCTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 742
BsrI ACTGG 3 cut(s) 289, 451, 622
BssAI RCCGGY 1 cut(s) 742
BssMI GATC 2 cut(s) 7, 643
BssNAI GTATAC 1 cut(s) 583
BssNI GRCGYC 1 cut(s) 480
Bst1107I GTATAC 1 cut(s) 583
Bst4CI ACNGT 1 cut(s) 563
BstACI GRCGYC 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 407
BstF5I GGATG 3 cut(s) 10, 451, 534
BstKTI GATC 2 cut(s) 10, 646
BstMAI GTCTC 1 cut(s) 6
BstMBI GATC 2 cut(s) 7, 643
BstNSI RCATGY 1 cut(s) 371
BstV1I GCAGC 4 cut(s) 244, 247, 414, 726
BstV2I GAAGAC 3 cut(s) 30, 483, 525
BstZ17I GTATAC 1 cut(s) 583
Bsu15I ATCGAT 1 cut(s) 47
BsuRI GGCC 1 cut(s) 450
BsuTUI ATCGAT 1 cut(s) 47
BtsCI GGATG 3 cut(s) 10, 451, 534
BtsIMutI CAGTG 2 cut(s) 458, 615
BveI ACCTGC 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 407
CciI TCATGA 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 742
ClaI ATCGAT 1 cut(s) 47
CviAII CATG 7 cut(s) 17, 182, 368, 539, 608, 641, 647
CviJI RGCY 7 cut(s) 224, 242, 409, 450, 624, 711, 739
CviKI_1 RGCY 7 cut(s) 224, 242, 409, 450, 624, 711, 739
DpnI GATC 2 cut(s) 9, 645
DpnII GATC 2 cut(s) 7, 643
EaeI YGGCCR 1 cut(s) 448
Eco31I GGTCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 240
FaeI CATG 7 cut(s) 20, 185, 371, 542, 611, 644, 650
FatI CATG 7 cut(s) 16, 181, 367, 538, 607, 640, 646
FauI CCCGC 1 cut(s) 589
FauNDI CATATG 1 cut(s) 84
FbaI TGATCA 1 cut(s) 643
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 4 cut(s) 258, 261, 403, 740
FokI GGATG 3 cut(s) 17, 458, 521
Fsp4HI GCNGC 4 cut(s) 258, 261, 403, 740
FspBI CTAG 2 cut(s) 236, 375
GluI GCNGC 4 cut(s) 258, 261, 403, 740
GsuI CTGGAG 2 cut(s) 306, 639
HaeIII GGCC 1 cut(s) 450
HapII CCGG 2 cut(s) 691, 743
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 7 cut(s) 20, 185, 371, 542, 611, 644, 650
HinfI GANTC 2 cut(s) 104, 651
HpaII CCGG 2 cut(s) 691, 743
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 559, 583
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 7 cut(s) 11, 212, 356, 641, 691, 732, 760
Hpy8I GTNNAC 2 cut(s) 559, 583
Hpy99I CGWCG 2 cut(s) 196, 485
HpyAV CCTTC 6 cut(s) 52, 56, 179, 320, 523, 729
HpyCH4III ACNGT 1 cut(s) 563
HpyCH4IV ACGT 3 cut(s) 202, 480, 677
HpyCH4V TGCA 4 cut(s) 141, 263, 402, 456
HpySE526I ACGT 3 cut(s) 202, 480, 677
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 7 cut(s) 20, 185, 371, 542, 611, 644, 650
Kpn2I TCCGGA 1 cut(s) 690
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 2 cut(s) 7, 643
Lsp1109I GCAGC 4 cut(s) 244, 247, 414, 726
LweI GCATC 1 cut(s) 543
MaeI CTAG 2 cut(s) 236, 375
MaeII ACGT 3 cut(s) 202, 480, 677
MaeIII GTNAC 4 cut(s) 291, 541, 563, 673
MalI GATC 2 cut(s) 9, 645
MboI GATC 2 cut(s) 7, 643
MboII GAAGA 9 cut(s) 32, 35, 178, 181, 487, 488, 520, 530, 780
MlsI TGGCCA 1 cut(s) 450
MluCI AATT 8 cut(s) 72, 122, 143, 157, 207, 390, 603, 781
MluNI TGGCCA 1 cut(s) 450
MlyI GAGTC 1 cut(s) 645
MnlI CCTC 8 cut(s) 111, 189, 304, 363, 435, 614, 623, 768
Mox20I TGGCCA 1 cut(s) 450
MroI TCCGGA 1 cut(s) 690
MroXI GAANNNNTTC 1 cut(s) 632
MscI TGGCCA 1 cut(s) 450
MseI TTAA 2 cut(s) 71, 389
MslI CAYNNNNRTG 1 cut(s) 645
Msp20I TGGCCA 1 cut(s) 450
MspI CCGG 2 cut(s) 691, 743
Mva1269I GAATGC 1 cut(s) 700
NdeI CATATG 1 cut(s) 84
NdeII GATC 2 cut(s) 7, 643
NlaIII CATG 7 cut(s) 20, 185, 371, 542, 611, 644, 650
NmuCI GTSAC 2 cut(s) 541, 673
NspI RCATGY 1 cut(s) 371
PagI TCATGA 1 cut(s) 640
PciI ACATGT 1 cut(s) 367
PctI GAATGC 1 cut(s) 700
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 4 cut(s) 259, 262, 404, 741
PleI GAGTC 1 cut(s) 645
PpsI GAGTC 1 cut(s) 645
PscI ACATGT 1 cut(s) 367
PsiI TTATAA 1 cut(s) 249
RseI CAYNNNNRTG 1 cut(s) 645
SaqAI TTAA 2 cut(s) 71, 389
SatI GCNGC 4 cut(s) 258, 261, 403, 740
Sau3AI GATC 2 cut(s) 7, 643
SchI GAGTC 1 cut(s) 645
SetI ASST 8 cut(s) 67, 99, 205, 331, 461, 483, 517, 680
SfaNI GCATC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 645
Sse9I AATT 8 cut(s) 72, 122, 143, 157, 207, 390, 603, 781
SsiI CCGC 1 cut(s) 596
SspMI CTAG 2 cut(s) 236, 375
TaaI ACNGT 1 cut(s) 563
TaiI ACGT 3 cut(s) 205, 483, 680
TaqI TCGA 3 cut(s) 10, 47, 483
TasI AATT 8 cut(s) 72, 122, 143, 157, 207, 390, 603, 781
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 2 cut(s) 71, 389
Tru9I TTAA 2 cut(s) 71, 389
TscAI CASTG 2 cut(s) 458, 622
TseFI GTSAC 2 cut(s) 541, 673
TseI GCWGC 4 cut(s) 257, 260, 402, 739
Tsp45I GTSAC 2 cut(s) 541, 673
TspDTI ATGAA 8 cut(s) 33, 71, 101, 149, 198, 596, 629, 765
TspRI CASTG 2 cut(s) 458, 622
XapI RAATTY 2 cut(s) 143, 603
XceI RCATGY 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 582
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 2 cut(s) 236, 375
ZraI GACGTC 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.