Prupe.7G224000_v2.0.a1

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0002989
Physical Location & Seq
Forward (+)
19928533 .. 19929405
873 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G224000.1

Sequence Viewer

Length: 873 bp
ATGCGCAAGAAGAATTGGCAGCAGCAGCCTTACAAGGCACTGAAGTTGAAGCCATTGTTGCACAAGAACCTGCAGTTGCAAGCCTCCAAAGCTCAACTCGGTTTCAAGAATCTAAAGTCATGGCAGCACATGACATGCCTGATCAACCGCAAGACTGAAGACCCCGGTTATGAAGACAAAGAGATGAAGGACAGTATCAAAGGTGCTCATGATCTTCTATATTTAAAGCATACAATTTTGGATTACAAAACAGCGAGAAAGTTGGAGCACATGAAGAGCAAAATTCTTTCTGGAATTGAGTTAAACTCACCCAGCGGAAGTAAGCAAAAGGCACTACGAATCCAAGAATCTGAAGCCACTAATGGTGCTGCACAAGGACGAAGAGAACTGCAATTTCAACAAGAAGGAGGAGGAGGTTTGTTCTATGGACACTATGCCCCTCCTGATTTGCCACCAATCACAAGCCTACGAAAATTAATCCAAGATTGCTGCAAGCCTTTTGAGAAACAGCTAACACCCAGTGATGTGAGTAAAACTCCCAGGCTCTGCTTGAACAAAGAATACGTTGAAAATCACATCAAACCAGTATTAAGAGACGGTGAAAACCCTAATGAAGGCATCGATGTGACAACTTATGACATGGAAGGCAAGGAGTACCCAATGAAGTTCACGACTTGGGGCACCGATCTTTATGTCCTTAGTAAAGGCTGGAAAACTTTCTGTCATGATGTTGGACTGGTTGAGACACAGGATTTTCTGACGTTGTGTGTTTTCCGCCATGCCAAGAATGGAGGCCTCTGCTTCGCAATCCATTCACGAAGGTTGCCTTTGTTCAAAAGCATCAAGAAAAGAAGACAAAGAAAGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

291

Amino Acids

33.43

Weight (kDa)

9.58

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 680
AciI CCGC 3 cut(s) 148, 315, 775
AcsI RAATTY 1 cut(s) 282
AcuI CTGAAG 3 cut(s) 62, 177, 372
AdeI CACNNNGTG 1 cut(s) 521
AfaI GTAC 1 cut(s) 656
AfiI CCNNNNNNNGG 1 cut(s) 614
AgsI TTSAA 6 cut(s) 49, 106, 398, 553, 569, 835
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 2 cut(s) 92, 511
AluI AGCT 2 cut(s) 92, 511
Alw21I GWGCWC 2 cut(s) 208, 270
Alw26I GTCTC 2 cut(s) 588, 737
AlwNI CAGNNNCTG 1 cut(s) 546
AoxI GGCC 1 cut(s) 793
ApeKI GCWGC 6 cut(s) 19, 22, 25, 124, 368, 489
ApoI RAATTY 1 cut(s) 282
AseI ATTAAT 1 cut(s) 476
Asp700I GAANNNNTTC 1 cut(s) 716
AspLEI GCGC 1 cut(s) 6
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 300, 611
BaeGI GKGCMC 1 cut(s) 683
BanI GGYRCC 1 cut(s) 680
BbsI GAAGAC 3 cut(s) 165, 180, 859
Bbv12I GWGCWC 2 cut(s) 208, 270
BbvI GCAGC 6 cut(s) 31, 34, 37, 136, 355, 476
BciT130I CCWGG 1 cut(s) 541
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 588, 737
BfmI CTRYAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 78
BisI GCNGC 6 cut(s) 20, 23, 26, 125, 369, 490
BlsI GCNGC 6 cut(s) 21, 24, 27, 126, 370, 491
Bme1390I CCNGG 2 cut(s) 165, 541
BmiI GGNNCC 1 cut(s) 682
BmrFI CCNGG 2 cut(s) 165, 541
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 2 cut(s) 627, 849
BmuI ACTGGG 1 cut(s) 513
BpiI GAAGAC 3 cut(s) 165, 180, 859
BplI GAGNNNNNCTC 4 cut(s) 290, 322, 520, 552
BpuMI CCSGG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 621
BsaJI CCNNGG 2 cut(s) 163, 539
Bsc4I CCNNNNNNNGG 1 cut(s) 614
Bse1I ACTGG 3 cut(s) 519, 584, 741
BseBI CCWGG 1 cut(s) 541
BseCI ATCGAT 1 cut(s) 621
BseDI CCNNGG 2 cut(s) 163, 539
BseLI CCNNNNNNNGG 1 cut(s) 614
BseNI ACTGG 3 cut(s) 519, 584, 741
BseRI GAGGAG 2 cut(s) 423, 426
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 6 cut(s) 31, 34, 37, 136, 355, 476
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 1 cut(s) 795
BshNI GGYRCC 1 cut(s) 680
BshVI ATCGAT 1 cut(s) 621
BsiHKAI GWGCWC 2 cut(s) 208, 270
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 614
BsmAI GTCTC 2 cut(s) 588, 737
BsmBI CGTCTC 1 cut(s) 588
BsnI GGCC 1 cut(s) 795
Bsp1286I GDGCHC 3 cut(s) 208, 270, 683
Bsp143I GATC 3 cut(s) 141, 211, 685
BspACI CCGC 3 cut(s) 148, 315, 775
BspANI GGCC 1 cut(s) 795
BspDI ATCGAT 1 cut(s) 621
BspHI TCATGA 2 cut(s) 208, 724
BspLI GGNNCC 1 cut(s) 682
BspMAI CTGCAG 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 269
BspT107I GGYRCC 1 cut(s) 680
BsrI ACTGG 3 cut(s) 519, 584, 741
BssECI CCNNGG 2 cut(s) 163, 539
BssMI GATC 3 cut(s) 141, 211, 685
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 2 cut(s) 194, 599
Bst6I CTCTTC 2 cut(s) 269, 376
BstC8I GCNNGC 2 cut(s) 81, 494
BstDEI CTNAG 1 cut(s) 698
BstENI CCTNNNNNAGG 1 cut(s) 612
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 3 cut(s) 144, 214, 688
BstMAI GTCTC 2 cut(s) 588, 737
BstMBI GATC 3 cut(s) 141, 211, 685
BstMWI GCNNNNNNNGC 3 cut(s) 25, 58, 89
BstNI CCWGG 1 cut(s) 541
BstNSI RCATGY 1 cut(s) 138
BstSCI CCNGG 2 cut(s) 163, 539
BstSFI CTRYAG 1 cut(s) 71
BstSLI GKGCMC 1 cut(s) 683
BstV1I GCAGC 6 cut(s) 31, 34, 37, 136, 355, 476
BstV2I GAAGAC 3 cut(s) 165, 180, 859
Bsu15I ATCGAT 1 cut(s) 621
BsuRI GGCC 1 cut(s) 795
BsuTUI ATCGAT 1 cut(s) 621
BtsIMutI CAGTG 2 cut(s) 38, 526
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 2 cut(s) 81, 494
CaiI CAGNNNCTG 1 cut(s) 546
CciI TCATGA 2 cut(s) 208, 724
CfoI GCGC 1 cut(s) 6
ClaI ATCGAT 1 cut(s) 621
Csp6I GTAC 1 cut(s) 655
CviAII CATG 8 cut(s) 120, 130, 135, 209, 271, 640, 725, 779
CviQI GTAC 1 cut(s) 655
DdeI CTNAG 1 cut(s) 698
DpnI GATC 3 cut(s) 143, 213, 687
DpnII GATC 3 cut(s) 141, 211, 685
DraI TTTAAA 1 cut(s) 225
DraIII CACNNNGTG 1 cut(s) 521
Eam1104I CTCTTC 2 cut(s) 269, 376
EarI CTCTTC 2 cut(s) 269, 376
EciI GGCGGA 1 cut(s) 764
Eco147I AGGCCT 1 cut(s) 795
Eco57I CTGAAG 3 cut(s) 62, 177, 372
EcoNI CCTNNNNNAGG 1 cut(s) 612
EcoRII CCWGG 1 cut(s) 539
Esp3I CGTCTC 1 cut(s) 588
FaeI CATG 8 cut(s) 123, 133, 138, 212, 274, 643, 728, 782
FalI AAGNNNNNCTT 2 cut(s) 811, 843
FatI CATG 8 cut(s) 119, 129, 134, 208, 270, 639, 724, 778
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 20, 23, 26, 125, 369, 490
Fsp4HI GCNGC 6 cut(s) 20, 23, 26, 125, 369, 490
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 1 cut(s) 5
GluI GCNGC 6 cut(s) 20, 23, 26, 125, 369, 490
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 1 cut(s) 795
HapII CCGG 1 cut(s) 165
HhaI GCGC 1 cut(s) 6
Hin1II CATG 8 cut(s) 123, 133, 138, 212, 274, 643, 728, 782
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 3 cut(s) 109, 339, 347
HpaII CCGG 1 cut(s) 165
HphI GGTGA 2 cut(s) 300, 611
Hpy166II GTNNAC 1 cut(s) 669
Hpy188I TCNGA 2 cut(s) 352, 759
Hpy188III TCNNGA 8 cut(s) 106, 209, 291, 443, 670, 725, 816, 844
Hpy8I GTNNAC 1 cut(s) 669
HpyAV CCTTC 5 cut(s) 181, 398, 608, 638, 813
HpyCH4III ACNGT 2 cut(s) 194, 599
HpyCH4IV ACGT 2 cut(s) 564, 761
HpyCH4V TGCA 6 cut(s) 61, 73, 79, 371, 391, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 58, 89
HpyF3I CTNAG 1 cut(s) 698
HpySE526I ACGT 2 cut(s) 564, 761
Hsp92II CATG 8 cut(s) 123, 133, 138, 212, 274, 643, 728, 782
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 3 cut(s) 141, 211, 685
LguI GCTCTTC 1 cut(s) 269
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 6 cut(s) 31, 34, 37, 136, 355, 476
LweI GCATC 2 cut(s) 627, 849
MaeII ACGT 2 cut(s) 564, 761
MaeIII GTNAC 1 cut(s) 625
MalI GATC 3 cut(s) 143, 213, 687
MboI GATC 3 cut(s) 141, 211, 685
MboII GAAGA 7 cut(s) 22, 170, 185, 206, 286, 393, 864
MhlI GDGCHC 3 cut(s) 208, 270, 683
MluCI AATT 7 cut(s) 13, 234, 282, 294, 392, 473, 868
MmeI TCCRAC 2 cut(s) 243, 712
MnlI CCTC 7 cut(s) 94, 401, 404, 407, 450, 785, 806
MroXI GAANNNNTTC 1 cut(s) 716
MseI TTAA 4 cut(s) 224, 302, 476, 590
MslI CAYNNNNRTG 1 cut(s) 623
MspA1I CMGCKG 1 cut(s) 315
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 2 cut(s) 165, 541
MvaI CCWGG 1 cut(s) 541
MwoI GCNNNNNNNGC 3 cut(s) 25, 58, 89
NciI CCSGG 1 cut(s) 165
NdeII GATC 3 cut(s) 141, 211, 685
NlaIII CATG 8 cut(s) 123, 133, 138, 212, 274, 643, 728, 782
NlaIV GGNNCC 1 cut(s) 682
NmuCI GTSAC 1 cut(s) 625
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 1 cut(s) 138
PagI TCATGA 2 cut(s) 208, 724
PceI AGGCCT 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 716
PfeI GAWTC 3 cut(s) 109, 339, 347
PkrI GCNGC 6 cut(s) 21, 24, 27, 126, 370, 491
PshBI ATTAAT 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 1 cut(s) 682
PsrI GAACNNNNNNTAC 2 cut(s) 545, 577
PstI CTGCAG 1 cut(s) 75
PstNI CAGNNNCTG 1 cut(s) 546
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
RseI CAYNNNNRTG 1 cut(s) 623
SapI GCTCTTC 1 cut(s) 269
SaqAI TTAA 4 cut(s) 224, 302, 476, 590
SatI GCNGC 6 cut(s) 20, 23, 26, 125, 369, 490
Sau3AI GATC 3 cut(s) 141, 211, 685
ScrFI CCNGG 2 cut(s) 165, 541
SduI GDGCHC 3 cut(s) 208, 270, 683
SetI ASST 8 cut(s) 72, 94, 205, 418, 513, 567, 764, 824
SfaNI GCATC 2 cut(s) 627, 849
SfcI CTRYAG 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 623
Sse9I AATT 7 cut(s) 13, 234, 282, 294, 392, 473, 868
SseBI AGGCCT 1 cut(s) 795
SsiI CCGC 3 cut(s) 148, 315, 775
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 2 cut(s) 163, 539
TaaI ACNGT 2 cut(s) 194, 599
TaiI ACGT 2 cut(s) 567, 764
TaqI TCGA 1 cut(s) 621
TasI AATT 7 cut(s) 13, 234, 282, 294, 392, 473, 868
TfiI GAWTC 3 cut(s) 109, 339, 347
Tru1I TTAA 4 cut(s) 224, 302, 476, 590
Tru9I TTAA 4 cut(s) 224, 302, 476, 590
TscAI CASTG 2 cut(s) 45, 526
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 6 cut(s) 19, 22, 25, 124, 368, 489
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 5 cut(s) 186, 200, 287, 627, 677
TspRI CASTG 2 cut(s) 45, 526
VspI ATTAAT 1 cut(s) 476
XagI CCTNNNNNAGG 1 cut(s) 612
XapI RAATTY 1 cut(s) 282
XceI RCATGY 1 cut(s) 138
XcmI CCANNNNNNNNNTGG 1 cut(s) 785
XmnI GAANNNNTTC 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.