Rroxscaffold_3G00262810

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002989
Physical Location & Seq
Forward (+)
56776456 .. 56777178
723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262810.1

Sequence Viewer

Length: 723 bp
ATGGCTAGGCTGACCAATGAAGAAGACGATGATATGATGAAGGCCGTTTTTAATCTTTGTCATATGAAGCATGTGCTTGTTGATTCTACGAAAGACATACAGATATTGGAGGATGCTAACCGCAAGTGTCATAGTCAAATTAGTGCTAAAGACAAACGAAAAACATTATTAATCAAAGAACCAGGAGATGAATGCATTGTTAATGTTGCCCAAGGCTTGCATAGAAACCAACGACTACAACAGGATCCTAAGAGAAAAGCTTTGCAAGTCACAGGACAAGAAGCCATTTACGCTGGACAAGTCTTAGCAAACTCAAACATATGTCAAGGTTTGTTCTATGGAAACTATGCCCCTCCTAATCTCCCACCAGTCCCACGCCTACGACAATTTATTGAAATTTGCAGTCAGCCGTTTGAGAAGCAATTAACATTTAGTGATGTGAAGGATGACCAATCAAGGCTAGCTATGAGCAAAGAGGATGTCCAAAAGCGCCTAATGCCATTGTTGAATGAGAATGAAGATCTTAGACGAGGAATCCAAGTCACAACTTATGATTTGGATGGTAAAGAGTATCCCATGGTGTTCAAAATTTGGGTGGAAAAGATTCATGTCTTGACCGGAGGATGGAAAGCTTTTTGCCATGATCATAAGCTGGTTGAGAAGCAAGATTTTGTGACCGTATGGATGTTTCAAATGCTGCAACCGGTAGCCTTTGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

240

Amino Acids

27.73

Weight (kDa)

7.04

Isoelectric Point (pI)

60.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 138 - 226 2.9e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 121
AclWI GGATC 2 cut(s) 239, 252
AcsI RAATTY 2 cut(s) 396, 588
AfiI CCNNNNNNNGG 1 cut(s) 624
AgeI ACCGGT 1 cut(s) 703
AgsI TTSAA 4 cut(s) 395, 508, 586, 692
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 4 cut(s) 260, 464, 632, 652
AluI AGCT 4 cut(s) 260, 464, 632, 652
AlwI GGATC 2 cut(s) 239, 252
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 697
ApoI RAATTY 2 cut(s) 396, 588
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
AseI ATTAAT 1 cut(s) 170
AsiGI ACCGGT 1 cut(s) 703
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 1 cut(s) 492
AsuNHI GCTAGC 1 cut(s) 460
BamHI GGATCC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 684
BccI CCATC 2 cut(s) 554, 618
BceAI ACGGC 2 cut(s) 29, 394
BciT130I CCWGG 1 cut(s) 183
BciVI GTATCC 1 cut(s) 582
BclI TGATCA 1 cut(s) 643
BfaI CTAG 2 cut(s) 6, 461
BfoI RGCGCY 1 cut(s) 493
BfuI GTATCC 1 cut(s) 582
BglII AGATCT 1 cut(s) 520
BisI GCNGC 1 cut(s) 698
BlsI GCNGC 1 cut(s) 699
Bme1390I CCNGG 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 1 cut(s) 103
BmtI GCTAGC 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 211, 576
BsaWI WCCGGW 2 cut(s) 617, 703
Bsc4I CCNNNNNNNGG 1 cut(s) 624
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 1 cut(s) 368
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 2 cut(s) 211, 576
BseGI GGATG 6 cut(s) 118, 451, 484, 565, 629, 690
BseLI CCNNNNNNNGG 1 cut(s) 624
BseNI ACTGG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 684
BshFI GGCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 703
BsiSI CCGG 2 cut(s) 618, 704
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 624
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 1 cut(s) 44
Bsp143I GATC 3 cut(s) 244, 520, 643
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 1 cut(s) 121
BspANI GGCC 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 246
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 2 cut(s) 239, 252
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 1 cut(s) 368
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 2 cut(s) 211, 576
BssMI GATC 3 cut(s) 244, 520, 643
BssT1I CCWWGG 2 cut(s) 211, 576
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 679
BstC8I GCNNGC 2 cut(s) 218, 462
BstDEI CTNAG 3 cut(s) 249, 304, 524
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 6 cut(s) 118, 451, 484, 565, 629, 690
BstH2I RGCGCY 1 cut(s) 493
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 3 cut(s) 247, 523, 646
BstMBI GATC 3 cut(s) 244, 520, 643
BstMWI GCNNNNNNNGC 2 cut(s) 290, 496
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 181
BstV1I GCAGC 1 cut(s) 684
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 244, 520
BstYI RGATCY 2 cut(s) 244, 520
BsuI GTATCC 1 cut(s) 582
BsuRI GGCC 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 576
BtsCI GGATG 6 cut(s) 118, 451, 484, 565, 629, 690
Cac8I GCNNGC 2 cut(s) 218, 462
CfoI GCGC 1 cut(s) 492
Cfr10I RCCGGY 1 cut(s) 703
CspAI ACCGGT 1 cut(s) 703
CviAII CATG 4 cut(s) 71, 577, 608, 641
DdeI CTNAG 3 cut(s) 249, 304, 524
DpnI GATC 3 cut(s) 246, 522, 645
DpnII GATC 3 cut(s) 244, 520, 643
Eco130I CCWWGG 2 cut(s) 211, 576
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 2 cut(s) 211, 576
EcoT22I ATGCAT 1 cut(s) 197
ErhI CCWWGG 2 cut(s) 211, 576
FaeI CATG 4 cut(s) 74, 580, 611, 644
FaqI GGGAC 1 cut(s) 356
FatI CATG 4 cut(s) 70, 576, 607, 640
FauNDI CATATG 2 cut(s) 63, 320
FbaI TGATCA 1 cut(s) 643
Fnu4HI GCNGC 1 cut(s) 698
FokI GGATG 6 cut(s) 125, 458, 491, 572, 636, 697
Fsp4HI GCNGC 1 cut(s) 698
FspBI CTAG 2 cut(s) 6, 461
GlaI GCGC 1 cut(s) 491
GluI GCNGC 1 cut(s) 698
HaeII RGCGCY 1 cut(s) 493
HaeIII GGCC 1 cut(s) 44
HapII CCGG 2 cut(s) 618, 704
HhaI GCGC 1 cut(s) 492
Hin1II CATG 4 cut(s) 74, 580, 611, 644
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 258, 630
HinfI GANTC 3 cut(s) 83, 534, 604
HpaII CCGG 2 cut(s) 618, 704
Hpy188III TCNNGA 1 cut(s) 613
HpyAV CCTTC 2 cut(s) 34, 436
HpyCH4III ACNGT 1 cut(s) 679
HpyCH4V TGCA 5 cut(s) 195, 220, 265, 402, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 496
HpyF3I CTNAG 3 cut(s) 249, 304, 524
Hsp92II CATG 4 cut(s) 74, 580, 611, 644
HspAI GCGC 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 3 cut(s) 244, 520, 643
LpnPI CCDG 9 cut(s) 168, 195, 227, 258, 279, 381, 631, 638, 717
Lsp1109I GCAGC 1 cut(s) 684
LweI GCATC 1 cut(s) 103
MaeI CTAG 2 cut(s) 6, 461
MaeIII GTNAC 3 cut(s) 268, 541, 673
MalI GATC 3 cut(s) 246, 522, 645
MboI GATC 3 cut(s) 244, 520, 643
MboII GAAGA 3 cut(s) 32, 35, 530
MflI RGATCY 2 cut(s) 244, 520
MluCI AATT 5 cut(s) 138, 386, 396, 422, 588
MnlI CCTC 5 cut(s) 103, 363, 469, 524, 614
Mph1103I ATGCAT 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 4 cut(s) 51, 170, 201, 425
MspI CCGG 2 cut(s) 618, 704
MspR9I CCNGG 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 290, 496
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 2 cut(s) 63, 320
NdeII GATC 3 cut(s) 244, 520, 643
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 4 cut(s) 74, 580, 611, 644
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 3 cut(s) 268, 541, 673
NsiI ATGCAT 1 cut(s) 197
NspI RCATGY 1 cut(s) 74
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 3 cut(s) 83, 534, 604
PinAI ACCGGT 1 cut(s) 703
PkrI GCNGC 1 cut(s) 699
PshBI ATTAAT 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 246
PsuI RGATCY 2 cut(s) 244, 520
SaqAI TTAA 4 cut(s) 51, 170, 201, 425
SatI GCNGC 1 cut(s) 698
Sau3AI GATC 3 cut(s) 244, 520, 643
ScrFI CCNGG 1 cut(s) 183
SetI ASST 5 cut(s) 262, 331, 466, 634, 654
SfaNI GCATC 1 cut(s) 103
Sse9I AATT 5 cut(s) 138, 386, 396, 422, 588
SsiI CCGC 1 cut(s) 121
SspMI CTAG 2 cut(s) 6, 461
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 2 cut(s) 211, 576
TaaI ACNGT 1 cut(s) 679
TasI AATT 5 cut(s) 138, 386, 396, 422, 588
TfiI GAWTC 3 cut(s) 83, 534, 604
Tru1I TTAA 4 cut(s) 51, 170, 201, 425
Tru9I TTAA 4 cut(s) 51, 170, 201, 425
TseFI GTSAC 3 cut(s) 268, 541, 673
TseI GCWGC 1 cut(s) 697
Tsp45I GTSAC 3 cut(s) 268, 541, 673
TspDTI ATGAA 6 cut(s) 33, 53, 80, 204, 531, 596
VspI ATTAAT 1 cut(s) 170
XapI RAATTY 2 cut(s) 396, 588
XceI RCATGY 1 cut(s) 74
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 2 cut(s) 6, 461
Zsp2I ATGCAT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.