FvH4_5g06160

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002989
Physical Location & Seq
Forward (+)
3622433 .. 3623152
720 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06160.t1

Sequence Viewer

Length: 720 bp
ATGGCTAGGCTTGCCAACGAAGAAGACGATGAGATGATGAAGGCCGTTTTTAACCTTTGTCATATGAAGCATGCACTTGTTGATTCTACAAAAGACAAATTATTTATCAAAGAACAAGGAGACGCATTCACTGTTAATGTTGCCCGAGGCTTGCCAAGAAACCAAAGACTACAACATGATGTCAAGAGAAAAGCTCTGCAAATCTCAGAACAAGAAGCCATTTATGTTGGACAAGGCTCAGCAAACTCAAACATACCTCAGGGTTTGTTCTATGGAAATTATGTCCCTCCTAGTCTCCCACCAGTCCCGAGTCTTCGGCAATTTATTGGAATCTGCAGTGAGCCGTTTGAGAAGCAATTAACACATACTGATGTGAAGAATGACCAAGCAAGGCTAGCTATGAGCAAAGAGCATGTCCAAAAGCGCCTAATACCATTGTTGAATGAGAATGAAGATCTGAGACGAGGAATCCAAGTCACAACTTATGATTCAGAAGGTAAATCGTATCCCATGGTGTTCAAGATTTGGGTGGGAAAGCTTCCTGTCTTGACGGGAGGATGGACAGCTTTTTGCCAAGATCATAATCTGGTTGAGATGCAAGATTTTGTCACTGTATGGATGTTTCGAAATGTTGCGACGGGTTGTCTTTGCTTTGTAATCGATTCGAGAAGGTTGCCGGTGCTTGAAACTATCAAGAGAAGGAGAACAAGGTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

240

Amino Acids

27.36

Weight (kDa)

8.95

Isoelectric Point (pI)

57.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 116 - 206 1.3e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 3 cut(s) 442, 520, 686
AhdI GACNNNNNGTC 1 cut(s) 642
AluBI AGCT 4 cut(s) 194, 398, 538, 566
AluI AGCT 4 cut(s) 194, 398, 538, 566
Alw26I GTCTC 3 cut(s) 114, 299, 454
Ama87I CYCGRG 2 cut(s) 144, 307
AoxI GGCC 1 cut(s) 42
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AspLEI GCGC 1 cut(s) 426
AsuII TTCGAA 1 cut(s) 625
AsuNHI GCTAGC 1 cut(s) 394
AvaI CYCGRG 2 cut(s) 144, 307
AxyI CCTNAGG 1 cut(s) 258
BbsI GAAGAC 2 cut(s) 30, 305
BccI CCATC 1 cut(s) 552
BceAI ACGGC 2 cut(s) 29, 328
BcgI CGANNNNNNTGC 2 cut(s) 655, 689
BciVI GTATCC 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 114, 299, 454
BfaI CTAG 3 cut(s) 6, 291, 395
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 427
BfuI GTATCC 1 cut(s) 516
BglII AGATCT 1 cut(s) 454
BlpI GCTNAGC 1 cut(s) 238
BmeRI GACNNNNNGTC 1 cut(s) 642
BmeT110I CYCGRG 2 cut(s) 144, 307
BmsI GCATC 1 cut(s) 585
BmtI GCTAGC 1 cut(s) 398
BpiI GAAGAC 2 cut(s) 30, 305
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu1102I GCTNAGC 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 625
Bsa29I ATCGAT 1 cut(s) 660
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 2 cut(s) 145, 510
Bse118I RCCGGY 1 cut(s) 676
Bse1I ACTGG 1 cut(s) 302
Bse21I CCTNAGG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 582
BseCI ATCGAT 1 cut(s) 660
BseDI CCNNGG 2 cut(s) 145, 510
BseGI GGATG 2 cut(s) 563, 624
BseJI GATNNNNATC 1 cut(s) 582
BseMII CTCAG 4 cut(s) 219, 252, 272, 449
BseNI ACTGG 1 cut(s) 302
BshFI GGCC 1 cut(s) 44
BshVI ATCGAT 1 cut(s) 660
BsiHKCI CYCGRG 2 cut(s) 144, 307
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 2 cut(s) 269, 290
BsmAI GTCTC 3 cut(s) 114, 299, 454
BsmBI CGTCTC 2 cut(s) 114, 454
BsmFI GGGAC 2 cut(s) 269, 290
BsmI GAATGC 1 cut(s) 125
BsnI GGCC 1 cut(s) 44
BsoBI CYCGRG 2 cut(s) 144, 307
Bsp119I TTCGAA 1 cut(s) 625
Bsp143I GATC 2 cut(s) 454, 577
Bsp1720I GCTNAGC 1 cut(s) 238
Bsp19I CCATGG 1 cut(s) 510
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 4 cut(s) 218, 251, 271, 450
BspDI ATCGAT 1 cut(s) 660
BspMAI CTGCAG 1 cut(s) 338
BspOI GCTAGC 1 cut(s) 398
BspT104I TTCGAA 1 cut(s) 625
BsrFI RCCGGY 1 cut(s) 676
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 676
BssECI CCNNGG 2 cut(s) 145, 510
BssMI GATC 2 cut(s) 454, 577
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 2 cut(s) 133, 613
BstBI TTCGAA 1 cut(s) 625
BstC8I GCNNGC 4 cut(s) 12, 72, 152, 396
BstDEI CTNAG 4 cut(s) 205, 238, 258, 458
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 2 cut(s) 563, 624
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 2 cut(s) 457, 580
BstMAI GTCTC 3 cut(s) 114, 299, 454
BstMBI GATC 2 cut(s) 454, 577
BstMWI GCNNNNNNNGC 2 cut(s) 11, 395
BstNSI RCATGY 2 cut(s) 74, 416
BstSFI CTRYAG 1 cut(s) 334
BstV2I GAAGAC 2 cut(s) 30, 305
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 660
Bsu36I CCTNAGG 1 cut(s) 258
BsuI GTATCC 1 cut(s) 516
BsuRI GGCC 1 cut(s) 44
BsuTUI ATCGAT 1 cut(s) 660
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 2 cut(s) 563, 624
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 3 cut(s) 129, 343, 609
Cac8I GCNNGC 4 cut(s) 12, 72, 152, 396
CfoI GCGC 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 676
ClaI ATCGAT 1 cut(s) 660
CseI GACGC 1 cut(s) 131
CviAII CATG 4 cut(s) 71, 176, 413, 511
DdeI CTNAG 4 cut(s) 205, 238, 258, 458
DpnI GATC 2 cut(s) 456, 579
DpnII GATC 2 cut(s) 454, 577
DriI GACNNNNNGTC 1 cut(s) 642
Eam1105I GACNNNNNGTC 1 cut(s) 642
Eco130I CCWWGG 1 cut(s) 510
Eco81I CCTNAGG 1 cut(s) 258
Eco88I CYCGRG 2 cut(s) 144, 307
EcoT14I CCWWGG 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 510
Esp3I CGTCTC 2 cut(s) 114, 454
FaeI CATG 4 cut(s) 74, 179, 416, 514
FaqI GGGAC 2 cut(s) 269, 290
FatI CATG 4 cut(s) 70, 175, 412, 510
FauNDI CATATG 1 cut(s) 63
FokI GGATG 2 cut(s) 570, 631
FspBI CTAG 3 cut(s) 6, 291, 395
GlaI GCGC 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 1 cut(s) 44
HapII CCGG 1 cut(s) 677
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 426
Hin1II CATG 4 cut(s) 74, 179, 416, 514
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 6 cut(s) 83, 310, 330, 468, 488, 662
HpaII CCGG 1 cut(s) 677
Hpy188I TCNGA 3 cut(s) 208, 459, 493
Hpy188III TCNNGA 6 cut(s) 184, 307, 520, 547, 666, 694
Hpy99I CGWCG 1 cut(s) 640
HpyAV CCTTC 4 cut(s) 34, 488, 663, 693
HpyCH4III ACNGT 2 cut(s) 133, 613
HpyCH4V TGCA 4 cut(s) 74, 199, 336, 598
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 395
HpyF3I CTNAG 4 cut(s) 205, 238, 258, 458
Hsp92II CATG 4 cut(s) 74, 179, 416, 514
HspAI GCGC 1 cut(s) 424
Kzo9I GATC 2 cut(s) 454, 577
LpnPI CCDG 5 cut(s) 245, 315, 555, 572, 690
LweI GCATC 1 cut(s) 585
MaeI CTAG 3 cut(s) 6, 291, 395
MaeIII GTNAC 2 cut(s) 475, 607
MalI GATC 2 cut(s) 456, 579
MboI GATC 2 cut(s) 454, 577
MboII GAAGA 5 cut(s) 32, 35, 305, 388, 464
MflI RGATCY 1 cut(s) 454
MluCI AATT 5 cut(s) 98, 277, 320, 356, 715
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 5 cut(s) 140, 267, 297, 458, 548
MseI TTAA 4 cut(s) 51, 135, 359, 718
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 1 cut(s) 677
Mva1269I GAATGC 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 11, 395
NcoI CCATGG 1 cut(s) 510
NdeI CATATG 1 cut(s) 63
NdeII GATC 2 cut(s) 454, 577
NheI GCTAGC 1 cut(s) 394
NlaIII CATG 4 cut(s) 74, 179, 416, 514
NmuCI GTSAC 2 cut(s) 475, 607
NspI RCATGY 2 cut(s) 74, 416
NspV TTCGAA 1 cut(s) 625
PaeI GCATGC 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 1 cut(s) 125
PfeI GAWTC 5 cut(s) 83, 330, 468, 488, 662
PleI GAGTC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 318
PstI CTGCAG 1 cut(s) 338
PsuI RGATCY 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 4 cut(s) 51, 135, 359, 718
Sau3AI GATC 2 cut(s) 454, 577
SchI GAGTC 1 cut(s) 319
SetI ASST 9 cut(s) 57, 196, 259, 400, 499, 540, 568, 674, 713
SfaNI GCATC 1 cut(s) 585
SfcI CTRYAG 1 cut(s) 334
SfuI TTCGAA 1 cut(s) 625
SmiMI CAYNNNNRTG 1 cut(s) 369
SphI GCATGC 1 cut(s) 74
Sse9I AATT 5 cut(s) 98, 277, 320, 356, 715
SspMI CTAG 3 cut(s) 6, 291, 395
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 133, 613
TaqI TCGA 3 cut(s) 625, 660, 665
TasI AATT 5 cut(s) 98, 277, 320, 356, 715
TfiI GAWTC 5 cut(s) 83, 330, 468, 488, 662
Tru1I TTAA 4 cut(s) 51, 135, 359, 718
Tru9I TTAA 4 cut(s) 51, 135, 359, 718
TscAI CASTG 3 cut(s) 136, 343, 616
TseFI GTSAC 2 cut(s) 475, 607
Tsp45I GTSAC 2 cut(s) 475, 607
TspDTI ATGAA 3 cut(s) 53, 80, 465
TspRI CASTG 3 cut(s) 136, 343, 616
XceI RCATGY 2 cut(s) 74, 416
XspI CTAG 3 cut(s) 6, 291, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.