RchiOBHm_Chr7g0192641

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002989
Physical Location & Seq
Reverse (-)
11063025 .. 11063983
959 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17221

Sequence Viewer

Length: 786 bp
ATGGCTAGGCTGACCAATGAAGAAGACGATGATATGATGAAGGCCGTTTTTAATCTTTGTCATATGAAGCATGTGCTTGTTGATTCTACAAAAGACATACAGATATTGGAGGATACTAACCGCAAGTGTCTTAGTCAAATTAGTACTAAAGACAAACGAAAAACATTATTAATCAAAGAACAAGGAGATGAATGCATTGTTAATGTTGCCCAAGGCTTGCATAGAAACCAACGACTACAACAGGATCCTAAGAGAAAAGCTTTGCAAGTCACAGGACAAGAAGCCATTTATGCTGGACAAGGCTTAACAAACTCAAACGTATGTCAAGGTTTGTTCTATGGAAACTATGCCCCTCCTAATCTCCCACCAGTCCCACGCCTACGACAATTTATTGAAATTTGCAGTCAGCCGTTTGAGAAGCAATTAACATTTAGTGATGTGAAGGATGACCAATCAAGGCTAGCTATGAGCAAAGAGGATGTCCAAAAGCGCCTAATGCCATTGTTGAATGAGAATGAAGATCTTAGACGAGGAATCCAAGTCACAACTTATGACTTGGATGGTAAAGAGTATCCCATGGTGTTCAAAATTTGGGTGGAAAAGATTCATGTCTTGACCGAAGGATGGAAAGCTTTTTGCCATGATCATAAGCTGGTTGAGAAGCAAGATTTTGTGACCGTATGGATGTTTCGAAATGCTGCAACCGGTAGCCTTTGCTTTGTAATTGATTTGAGAAGGCTGCCAGTGCTTGAAACCATCAAGAGAAGAAGAACAAGGCGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

261

Amino Acids

30.41

Weight (kDa)

8.9

Isoelectric Point (pI)

63.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 138 - 232 1.9e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 121
AclWI GGATC 2 cut(s) 239, 252
AcsI RAATTY 2 cut(s) 396, 588
AfaI GTAC 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 624
AgeI ACCGGT 1 cut(s) 704
AgsI TTSAA 4 cut(s) 395, 508, 586, 752
AluBI AGCT 4 cut(s) 260, 464, 632, 652
AluI AGCT 4 cut(s) 260, 464, 632, 652
AlwI GGATC 2 cut(s) 239, 252
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 2 cut(s) 698, 739
ApoI RAATTY 2 cut(s) 396, 588
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
AseI ATTAAT 1 cut(s) 170
AsiGI ACCGGT 1 cut(s) 704
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 1 cut(s) 492
AsuII TTCGAA 1 cut(s) 691
AsuNHI GCTAGC 1 cut(s) 460
BamHI GGATCC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 685, 726
BccI CCATC 3 cut(s) 554, 618, 764
BceAI ACGGC 2 cut(s) 29, 394
BciVI GTATCC 2 cut(s) 106, 582
BclI TGATCA 1 cut(s) 643
BfaI CTAG 2 cut(s) 6, 461
BfoI RGCGCY 1 cut(s) 493
BfuI GTATCC 2 cut(s) 106, 582
BglII AGATCT 1 cut(s) 520
BisI GCNGC 2 cut(s) 699, 740
BlsI GCNGC 2 cut(s) 700, 741
BmcAI AGTACT 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 246
BmtI GCTAGC 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 30
Bpu14I TTCGAA 1 cut(s) 691
BsaJI CCNNGG 2 cut(s) 211, 576
BsaWI WCCGGW 1 cut(s) 704
Bsc4I CCNNNNNNNGG 1 cut(s) 624
Bse118I RCCGGY 1 cut(s) 704
Bse1I ACTGG 2 cut(s) 368, 743
BseDI CCNNGG 2 cut(s) 211, 576
BseGI GGATG 5 cut(s) 451, 484, 565, 629, 690
BseLI CCNNNNNNNGG 1 cut(s) 624
BseNI ACTGG 2 cut(s) 368, 743
BseXI GCAGC 2 cut(s) 685, 726
BshFI GGCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 704
BsiSI CCGG 1 cut(s) 705
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 624
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 1 cut(s) 44
Bsp119I TTCGAA 1 cut(s) 691
Bsp143I GATC 3 cut(s) 244, 520, 643
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 1 cut(s) 121
BspANI GGCC 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 246
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 2 cut(s) 239, 252
BspT104I TTCGAA 1 cut(s) 691
BsrFI RCCGGY 1 cut(s) 704
BsrI ACTGG 2 cut(s) 368, 743
BssAI RCCGGY 1 cut(s) 704
BssECI CCNNGG 2 cut(s) 211, 576
BssMI GATC 3 cut(s) 244, 520, 643
BssT1I CCWWGG 2 cut(s) 211, 576
Bst4CI ACNGT 1 cut(s) 679
BstBI TTCGAA 1 cut(s) 691
BstC8I GCNNGC 2 cut(s) 218, 462
BstDEI CTNAG 3 cut(s) 131, 249, 524
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 5 cut(s) 451, 484, 565, 629, 690
BstH2I RGCGCY 1 cut(s) 493
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 3 cut(s) 247, 523, 646
BstMBI GATC 3 cut(s) 244, 520, 643
BstMWI GCNNNNNNNGC 3 cut(s) 290, 496, 745
BstNSI RCATGY 1 cut(s) 74
BstV1I GCAGC 2 cut(s) 685, 726
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 244, 520
BstYI RGATCY 2 cut(s) 244, 520
BsuI GTATCC 2 cut(s) 106, 582
BsuRI GGCC 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 576
BtsCI GGATG 5 cut(s) 451, 484, 565, 629, 690
BtsIMutI CAGTG 1 cut(s) 750
Cac8I GCNNGC 2 cut(s) 218, 462
CfoI GCGC 1 cut(s) 492
Cfr10I RCCGGY 1 cut(s) 704
Csp6I GTAC 1 cut(s) 144
CspAI ACCGGT 1 cut(s) 704
CviAII CATG 4 cut(s) 71, 577, 608, 641
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 3 cut(s) 131, 249, 524
DpnI GATC 3 cut(s) 246, 522, 645
DpnII GATC 3 cut(s) 244, 520, 643
Eco130I CCWWGG 2 cut(s) 211, 576
EcoT14I CCWWGG 2 cut(s) 211, 576
EcoT22I ATGCAT 1 cut(s) 197
ErhI CCWWGG 2 cut(s) 211, 576
FaeI CATG 4 cut(s) 74, 580, 611, 644
FaqI GGGAC 1 cut(s) 356
FatI CATG 4 cut(s) 70, 576, 607, 640
FauNDI CATATG 1 cut(s) 63
FbaI TGATCA 1 cut(s) 643
Fnu4HI GCNGC 2 cut(s) 699, 740
FokI GGATG 5 cut(s) 458, 491, 572, 636, 697
Fsp4HI GCNGC 2 cut(s) 699, 740
FspBI CTAG 2 cut(s) 6, 461
GlaI GCGC 1 cut(s) 491
GluI GCNGC 2 cut(s) 699, 740
HaeII RGCGCY 1 cut(s) 493
HaeIII GGCC 1 cut(s) 44
HapII CCGG 1 cut(s) 705
HhaI GCGC 1 cut(s) 492
Hin1II CATG 4 cut(s) 74, 580, 611, 644
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 258, 630
HinfI GANTC 3 cut(s) 83, 534, 604
HpaII CCGG 1 cut(s) 705
Hpy188III TCNNGA 2 cut(s) 613, 760
HpyAV CCTTC 4 cut(s) 34, 436, 614, 729
HpyCH4III ACNGT 1 cut(s) 679
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 5 cut(s) 195, 220, 265, 402, 701
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 496, 745
HpyF3I CTNAG 3 cut(s) 131, 249, 524
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 4 cut(s) 74, 580, 611, 644
HspAI GCGC 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 3 cut(s) 244, 520, 643
LpnPI CCDG 7 cut(s) 227, 258, 279, 381, 638, 718, 756
Lsp1109I GCAGC 2 cut(s) 685, 726
MaeI CTAG 2 cut(s) 6, 461
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 3 cut(s) 268, 541, 673
MalI GATC 3 cut(s) 246, 522, 645
MboI GATC 3 cut(s) 244, 520, 643
MboII GAAGA 5 cut(s) 32, 35, 530, 777, 780
MflI RGATCY 2 cut(s) 244, 520
MluCI AATT 7 cut(s) 138, 386, 396, 422, 588, 723, 781
MnlI CCTC 4 cut(s) 103, 363, 469, 524
Mph1103I ATGCAT 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 5 cut(s) 51, 170, 201, 305, 425
MspI CCGG 1 cut(s) 705
Mva1269I GAATGC 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 290, 496, 745
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 1 cut(s) 63
NdeII GATC 3 cut(s) 244, 520, 643
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 4 cut(s) 74, 580, 611, 644
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 3 cut(s) 268, 541, 673
NsiI ATGCAT 1 cut(s) 197
NspI RCATGY 1 cut(s) 74
NspV TTCGAA 1 cut(s) 691
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 3 cut(s) 83, 534, 604
PinAI ACCGGT 1 cut(s) 704
PkrI GCNGC 2 cut(s) 700, 741
PshBI ATTAAT 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 246
PsuI RGATCY 2 cut(s) 244, 520
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
SaqAI TTAA 5 cut(s) 51, 170, 201, 305, 425
SatI GCNGC 2 cut(s) 699, 740
Sau3AI GATC 3 cut(s) 244, 520, 643
ScaI AGTACT 1 cut(s) 145
SetI ASST 6 cut(s) 262, 321, 331, 466, 634, 654
SfuI TTCGAA 1 cut(s) 691
Sse9I AATT 7 cut(s) 138, 386, 396, 422, 588, 723, 781
SsiI CCGC 1 cut(s) 121
SspMI CTAG 2 cut(s) 6, 461
StyI CCWWGG 2 cut(s) 211, 576
TaaI ACNGT 1 cut(s) 679
TaiI ACGT 1 cut(s) 321
TaqI TCGA 1 cut(s) 691
TaqII GACCGA 1 cut(s) 632
TasI AATT 7 cut(s) 138, 386, 396, 422, 588, 723, 781
TatI WGTACW 1 cut(s) 143
TfiI GAWTC 3 cut(s) 83, 534, 604
Tru1I TTAA 5 cut(s) 51, 170, 201, 305, 425
Tru9I TTAA 5 cut(s) 51, 170, 201, 305, 425
TscAI CASTG 1 cut(s) 750
TseFI GTSAC 3 cut(s) 268, 541, 673
TseI GCWGC 2 cut(s) 698, 739
Tsp45I GTSAC 3 cut(s) 268, 541, 673
TspDTI ATGAA 6 cut(s) 33, 53, 80, 204, 531, 596
TspRI CASTG 1 cut(s) 750
VspI ATTAAT 1 cut(s) 170
XapI RAATTY 2 cut(s) 396, 588
XceI RCATGY 1 cut(s) 74
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 2 cut(s) 6, 461
ZrmI AGTACT 1 cut(s) 145
Zsp2I ATGCAT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.