FvH4_1g24261

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002989
Physical Location & Seq
Forward (+)
16137015 .. 16138071
1057 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24261.t1

Sequence Viewer

Length: 738 bp
ATGATCAGTCTTACCGATGAACAAGATATCAAGGCCGCTTTCAATCTTTGTTTTATGAAGCATAAGATTATTGATTCTGAAGAGGAGATACAGAAGTTGGAGGACACTAAGCTCAAGTCTCTTGGCCAAACTAGTAAATACCCGAATGCCGAAGCCAAACCAAGTGCACAGGAAATCCGAAAAAAAGAAGAATTCACTGTCAATCTACAACTAGGTCTTAAGCGGAAAGCAGTGCAAGTCGAAGCCATGTATGTTGCACAAGCAGCAAAACCACATGAAGGTTTGTTCTATGGAAACTATACCCCTCCTAATCTCCCACCGGTCCCAAGCCTACACCAATTTATCGGAATTTGCAGTGAGCCGTTTGAGAAGCAATTGACACCCAGTGATGTGAGGGATGGGCAGTCGAGGCTAGCTATGAGCAAAGAGTACGTGCAAAAGCGCCTAATGCCATTGTTGAATGAGAATGAAGATCTAAACGAAGGAATTGACGTGATGACTTATGATTTGGCTGGCAAGGAGTACCCCATGAAGTTCAGAACTTGGGTGTCCAAGATTCATGTGTTAACTGCAGGATGGAAAGCTTTCCGCAAGGATCATAAATTGGTTGAAAACCAACATTGGGTCACTGTGTGGATGTTTCGGAATATTGCAACCGGGAAGCTTTGCTTTGTAATCGAGGTGAGAAAGTTGCCTGTGTTTGATGCCATCAAGAGAAGAAGAACAAGGCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

246

Amino Acids

28.4

Weight (kDa)

9.18

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 122 - 206 1.4e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 36, 223, 589
AclWI GGATC 1 cut(s) 603
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 2 cut(s) 191, 348
AcuI CTGAAG 1 cut(s) 99
AdeI CACNNNGTG 2 cut(s) 386, 633
AfaI GTAC 2 cut(s) 431, 524
AfiI CCNNNNNNNGG 2 cut(s) 278, 622
AflII CTTAAG 1 cut(s) 218
AgeI ACCGGT 1 cut(s) 319
AgsI TTSAA 3 cut(s) 43, 460, 611
AhlI ACTAGT 1 cut(s) 131
AjiI CACGTC 1 cut(s) 493
AluBI AGCT 4 cut(s) 112, 416, 584, 664
AluI AGCT 4 cut(s) 112, 416, 584, 664
Alw21I GWGCWC 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 123
Alw44I GTGCAC 1 cut(s) 165
AlwI GGATC 1 cut(s) 603
AoxI GGCC 2 cut(s) 33, 124
ApaLI GTGCAC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 263
ApoI RAATTY 2 cut(s) 191, 348
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
AsiGI ACCGGT 1 cut(s) 319
Asp700I GAANNNNTTC 1 cut(s) 584
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 658
AsuHPI GGTGA 1 cut(s) 694
AsuNHI GCTAGC 1 cut(s) 412
AvaII GGWCC 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 169
BaeI ACNNNNGTAYC 2 cut(s) 80, 113
BalI TGGCCA 1 cut(s) 126
BarI GAAGNNNNNNTAC 2 cut(s) 72, 104
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 275
BccI CCATC 3 cut(s) 392, 570, 716
BceAI ACGGC 1 cut(s) 346
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 658
BcoDI GTCTC 1 cut(s) 123
BcuI ACTAGT 1 cut(s) 131
BfaI CTAG 3 cut(s) 132, 212, 413
BfmI CTRYAG 1 cut(s) 570
BfoI RGCGCY 1 cut(s) 445
BfrI CTTAAG 1 cut(s) 218
BglII AGATCT 1 cut(s) 472
BisI GCNGC 2 cut(s) 36, 264
BlsI GCNGC 2 cut(s) 37, 265
Bme1390I CCNGG 1 cut(s) 658
Bme18I GGWCC 1 cut(s) 322
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 658
BmrI ACTGGG 1 cut(s) 378
BmsI GCATC 1 cut(s) 694
BmtI GCTAGC 1 cut(s) 416
BmuI ACTGGG 1 cut(s) 378
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 658
BsaAI YACGTR 1 cut(s) 433
BsaWI WCCGGW 1 cut(s) 319
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 622
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 384
BseGI GGATG 3 cut(s) 403, 581, 642
BseLI CCNNNNNNNGG 2 cut(s) 278, 622
BseNI ACTGG 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 1 cut(s) 275
BshFI GGCC 2 cut(s) 35, 126
BshTI ACCGGT 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 169
BsiSI CCGG 2 cut(s) 320, 657
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 278, 622
BsmAI GTCTC 1 cut(s) 123
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 151
BsnI GGCC 2 cut(s) 35, 126
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 3 cut(s) 3, 472, 595
BspACI CCGC 3 cut(s) 36, 223, 589
BspANI GGCC 2 cut(s) 35, 126
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 574
BspOI GCTAGC 1 cut(s) 416
BspPI GGATC 1 cut(s) 603
BspTI CTTAAG 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 1 cut(s) 384
BssAI RCCGGY 1 cut(s) 319
BssMI GATC 3 cut(s) 3, 472, 595
Bst4CI ACNGT 2 cut(s) 199, 631
Bst6I CTCTTC 1 cut(s) 75
BstAFI CTTAAG 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 2 cut(s) 414, 514
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 3 cut(s) 403, 581, 642
BstH2I RGCGCY 1 cut(s) 445
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 3 cut(s) 6, 475, 598
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 3 cut(s) 3, 472, 595
BstMWI GCNNNNNNNGC 3 cut(s) 263, 409, 448
BstSCI CCNGG 1 cut(s) 656
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 275
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuRI GGCC 2 cut(s) 35, 126
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 3 cut(s) 403, 581, 642
BtsI GCAGTG 2 cut(s) 237, 361
BtsIMutI CAGTG 5 cut(s) 195, 237, 361, 391, 627
Cac8I GCNNGC 2 cut(s) 414, 514
CfoI GCGC 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 430, 523
CspAI ACCGGT 1 cut(s) 319
CviAII CATG 4 cut(s) 247, 275, 529, 560
CviQI GTAC 2 cut(s) 430, 523
DdeI CTNAG 1 cut(s) 108
DpnI GATC 3 cut(s) 5, 474, 597
DpnII GATC 3 cut(s) 3, 472, 595
DraIII CACNNNGTG 2 cut(s) 386, 633
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 99
EcoRI GAATTC 1 cut(s) 191
EcoRV GATATC 1 cut(s) 28
FaeI CATG 4 cut(s) 250, 278, 532, 563
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FaqI GGGAC 1 cut(s) 308
FatI CATG 4 cut(s) 246, 274, 528, 559
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 36, 264
FokI GGATG 3 cut(s) 410, 588, 649
Fsp4HI GCNGC 2 cut(s) 36, 264
FspBI CTAG 3 cut(s) 132, 212, 413
GlaI GCGC 1 cut(s) 443
GluI GCNGC 2 cut(s) 36, 264
HaeII RGCGCY 1 cut(s) 445
HaeIII GGCC 2 cut(s) 35, 126
HapII CCGG 2 cut(s) 320, 657
HhaI GCGC 1 cut(s) 444
Hin1II CATG 4 cut(s) 250, 278, 532, 563
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HindIII AAGCTT 2 cut(s) 582, 662
HinfI GANTC 2 cut(s) 74, 556
HpaI GTTAAC 1 cut(s) 567
HpaII CCGG 2 cut(s) 320, 657
HphI GGTGA 1 cut(s) 694
Hpy166II GTNNAC 2 cut(s) 167, 567
Hpy188I TCNGA 5 cut(s) 79, 179, 347, 539, 645
Hpy188III TCNNGA 1 cut(s) 712
Hpy8I GTNNAC 2 cut(s) 167, 567
HpyAV CCTTC 2 cut(s) 272, 476
HpyCH4III ACNGT 2 cut(s) 199, 631
HpyCH4IV ACGT 2 cut(s) 432, 492
HpyCH4V TGCA 7 cut(s) 167, 235, 257, 354, 436, 572, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 409, 448
HpyF3I CTNAG 1 cut(s) 108
HpySE526I ACGT 2 cut(s) 432, 492
Hsp92II CATG 4 cut(s) 250, 278, 532, 563
HspAI GCGC 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 567
Kzo9I GATC 3 cut(s) 3, 472, 595
LpnPI CCDG 7 cut(s) 155, 333, 397, 498, 558, 670, 708
Lsp1109I GCAGC 1 cut(s) 275
LweI GCATC 1 cut(s) 694
MaeI CTAG 3 cut(s) 132, 212, 413
MaeII ACGT 2 cut(s) 432, 492
MaeIII GTNAC 1 cut(s) 625
MalI GATC 3 cut(s) 5, 474, 597
MboI GATC 3 cut(s) 3, 472, 595
MboII GAAGA 5 cut(s) 92, 200, 482, 729, 732
MfeI CAATTG 1 cut(s) 374
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 169
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 7 cut(s) 191, 338, 348, 374, 486, 602, 733
MluNI TGGCCA 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 6 cut(s) 76, 94, 315, 387, 402, 673
Mox20I TGGCCA 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 584
MscI TGGCCA 1 cut(s) 126
MseI TTAA 2 cut(s) 219, 566
Msp20I TGGCCA 1 cut(s) 126
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 2 cut(s) 320, 657
MspR9I CCNGG 1 cut(s) 658
MunI CAATTG 1 cut(s) 374
Mva1269I GAATGC 1 cut(s) 151
MwoI GCNNNNNNNGC 3 cut(s) 263, 409, 448
NciI CCSGG 1 cut(s) 658
NdeII GATC 3 cut(s) 3, 472, 595
NheI GCTAGC 1 cut(s) 412
NlaIII CATG 4 cut(s) 250, 278, 532, 563
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 1 cut(s) 625
PctI GAATGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 2 cut(s) 74, 556
PinAI ACCGGT 1 cut(s) 319
PkrI GCNGC 2 cut(s) 37, 265
Ppu21I YACGTR 1 cut(s) 433
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 322
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 2 cut(s) 431, 524
RsaNI GTAC 2 cut(s) 430, 523
SaqAI TTAA 2 cut(s) 219, 566
SatI GCNGC 2 cut(s) 36, 264
Sau3AI GATC 3 cut(s) 3, 472, 595
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 658
SduI GDGCHC 1 cut(s) 169
SetI ASST 9 cut(s) 114, 217, 283, 418, 435, 495, 586, 666, 684
SfaNI GCATC 1 cut(s) 694
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 1 cut(s) 322
SmlI CTYRAG 2 cut(s) 113, 218
SmoI CTYRAG 2 cut(s) 113, 218
SpeI ACTAGT 1 cut(s) 131
Sse9I AATT 7 cut(s) 191, 338, 348, 374, 486, 602, 733
SsiI CCGC 3 cut(s) 36, 223, 589
SspI AATATT 1 cut(s) 649
SspMI CTAG 3 cut(s) 132, 212, 413
StyD4I CCNGG 1 cut(s) 656
TaaI ACNGT 2 cut(s) 199, 631
TaiI ACGT 2 cut(s) 435, 495
TaqI TCGA 3 cut(s) 240, 407, 678
TasI AATT 7 cut(s) 191, 338, 348, 374, 486, 602, 733
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 2 cut(s) 74, 556
Tru1I TTAA 2 cut(s) 219, 566
Tru9I TTAA 2 cut(s) 219, 566
TscAI CASTG 5 cut(s) 202, 237, 361, 391, 634
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 6 cut(s) 33, 71, 291, 483, 545, 548
TspRI CASTG 5 cut(s) 202, 237, 361, 391, 634
Vha464I CTTAAG 1 cut(s) 218
VneI GTGCAC 1 cut(s) 165
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 2 cut(s) 191, 348
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 3 cut(s) 132, 212, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.