Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0001248

Physical Location & Seq

Genomic Coordinates Reverse (-)

16761693 .. 16766728

Gene Structure 5036 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g24970.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGGGACAGAACCAAGTAGATGCATCACAAATGCAAGGGATGGATTCAGGGGAAAAACGACTTAATGAGCTTGGTTACAAGCAAGAACTCAGAAGAGAAATGACGTTGTTCAAAACTCTTGCAATATCATTCTCGACGATGACACTTTTCACCGGAATCACTCCTTTGTATGGTTCAAGCCTTGCATATGGAGGTCCTGCAACCCTTGTGTGGGGTTGGGTGGTAGTATCATTTTTCACCTGGTTTGTAGGACTTGCAATGGCAGAGATCTGTTCATCTTTCCCAACTACAGGATCTCTTTACTTCTGGGCTGCTCACTTAGCTGGTCCCAAATGGGGTCCTCTTGCATCATGGTGTTGTGCTTGGCTTGAGACCATAGGGCTCATTGCCGGCATAGGTACTCAGGCATATGCAGGATCTCAAACACTACAAAGTATCATATTACTATCCACCGGGACAAATAAAGGTGGAGGGTATTTTGCACCAAAGTGGTTATTCTTATGCATGTACATTGGCCTCACTCTTATATGGGCCTTTCTCAACACATTTGCGTTAGAAGTGATTGCCTTCATTGATATAATATCGATATGGTGGCAGGTCGTTGGAGGGACAATTATTGTTATATTGCTTCCCCTTGTAGCACTTACAACACAGTCTGCGTCATACGTGTTCACAAACTTCGATGTGGCCCCTGAGCTGACAGGAATATCAAGCAAGCCTTATGCAGTGATTCTATCCTTTCTTGTTAGTCAGTATTCATTATATGGATATGATGCTGCAGCACATCTAACTGAGGAAACCAGAGGAGCAGACAAGAATGGTCCTATTGCTATTCTAACAAGCATCGGTATCATTTCAGTGTTTGGATGGGCATACATCTTGGCCCTCACTTTCAGCATTCAGGATTTTAGCTACCTGTATGATCCCACCAATGAGACTGCAGGAGCATTTGTGCCTGCTCAAATTCTCTATGATGCATTTCATGGGAGGTATCACAGTTCCACTGGAGCCATCATTTTACTATGCATCATTTGGGGTTCATTCTTCTTTGGTGGACTTTCAATTACCACTAGTGCTGCGAGAGTGGTGTATGCTCTATCAAGAGACCAAGGGGTTCCATTCTCTTCCGTATGGCGAAAAATACACCCAAAACACAAAGTTCCTTCCAATGCTGTGTGGCTCTGTGCAGCCATCTGCATTCTCCTTGGACTTCCAATCTTAAAAGTCAATGTAGTCTTTGTTGCCATAACTTCCATATGTACGATTGGATGGGTAGGAGGTTATGCAGTTCCGATCTTTGCAAGAATGGTAATGCCTGAGAAGAACTTCAAAGCCGGGCCATTTTATTTGGGCAGTTTCAGCAGACCAATATGCTTGGTCGCCTTCCTGTGGATTTGTTACACTTGTTCTGTCTTCCTCCTCCCCACTTTCTACCCAATTTCCTGGAATACATTCAATTATGCACCTGTTGCTTTGAGTGTGGGTTTAGGTCTGATAATGCTTTGGTGGGTGTTAAGTGCAAGGAAGTGGTTCAAAGGACCTGTTAGAAACATCGAAACTGATCATAGTGGAAAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015179 GO:0015291 GO:0015297 GO:0015318 GO:0015711 GO:0015807 GO:0015849 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1902475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2

Protein Analysis

527

Amino Acids

57.75

Weight (kDa)

8.52

Isoelectric Point (pI)

34.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	35 - 491	1.3e-52	Amino acid permease
AA_permease	PF00324	57 - 505	2.9e-18	Amino acid permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001248)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g24970 FvH4_2g27760 FvH4_2g27770 FvH4_2g27771
malus_domestica	MD00G1162300.v1.1 MD01G1000200.v1.1
prunus_persica	Prupe.1G490100_v2.0.a1 Prupe.1G490200_v2.0.a1 Prupe.1G490200_v2.0.a1 Prupe.1G490300_v2.0.a1 Prupe.4G276400_v2.0.a1 Prupe.4G276400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0122601 RchiOBHm_Chr6g0296631 RchiOBHm_Chr6g0296641 RchiOBHm_Chr6g0296651
rosa_laevigata	RLG00000011673 RLG00000011674 RLG00000011675 RLG00000018694
rosa_multiflora	Rmu_sc0001741.1_g000017 Rmu_sc0001741.1_g000018 Rmu_sc0003133.1_g000008
rosa_roxburghii	Rroxscaffold_7G00171300 Rroxscaffold_7G00171310 Rroxscaffold_7G00171320 Rroxscaffold_7G00178780 Rroxscaffold_7G00178790
rosa_rugosa	Rorug02G0240000 Rorug06G0263800 Rorug06G0263900 Rorug06G0263900
rosa_samantha	Rh2AG298500 Rh2BG307300 Rh2CG285400 Rh2DG322100 Rh6AG375200 Rh6AG375300 Rh6AG375400 Rh6AG375500 Rh6BG383700 Rh6BG383800 Rh6BG383900 Rh6CG388900 Rh6CG389000 Rh6CG389100 Rh6DG376200 Rh6DG376300 Rh6DG376400
rosa_wichuraiana	Rw2G023730 Rw6G032730 Rw6G032740 Rw6G032750 Rw6G032820 Rw6G032830 Rw6G032840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	586
AclWI	GGATC	3 cut(s)	301, 424, 917
AcsI	RAATTY	1 cut(s)	963
AfaI	GTAC	3 cut(s)	400, 509, 1261
AfiI	CCNNNNNNNGG	6 cut(s)	170, 210, 211, 290, 335, 1389
AflIII	ACRYGT	1 cut(s)	666
AgsI	TTSAA	6 cut(s)	112, 177, 1062, 1330, 1456, 1534
AhlI	ACTAGT	1 cut(s)	1070
AjnI	CCWGG	2 cut(s)	239, 1442
AleI	CACNNNNGTG	1 cut(s)	487
AluBI	AGCT	4 cut(s)	70, 323, 697, 912
AluI	AGCT	4 cut(s)	70, 323, 697, 912
Alw26I	GTCTC	3 cut(s)	365, 929, 1098
AlwI	GGATC	3 cut(s)	301, 424, 917
AoxI	GGCC	5 cut(s)	514, 531, 687, 882, 1337
ApeKI	GCWGC	5 cut(s)	311, 776, 779, 1076, 1187
ApoI	RAATTY	1 cut(s)	963
Asp700I	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
AspS9I	GGNCC	9 cut(s)	194, 326, 338, 531, 688, 821, 883, 1337, 1538
AsuC2I	CCSGG	2 cut(s)	454, 1336
AsuHPI	GGTGA	2 cut(s)	142, 229
AvaII	GGWCC	5 cut(s)	194, 326, 338, 821, 1538
BanII	GRGCYC	1 cut(s)	384
BbsI	GAAGAC	1 cut(s)	1405
BbvI	GCAGC	5 cut(s)	298, 763, 791, 1063, 1199
BccI	CCATC	5 cut(s)	34, 861, 1019, 1199, 1263
BciT130I	CCWGG	2 cut(s)	241, 1444
BclI	TGATCA	1 cut(s)	1561
BcnI	CCSGG	2 cut(s)	454, 1336
BcoDI	GTCTC	3 cut(s)	365, 929, 1098
BcuI	ACTAGT	1 cut(s)	1070
BfaI	CTAG	1 cut(s)	1071
BfmI	CTRYAG	3 cut(s)	288, 777, 939
BfuAI	ACCTGC	1 cut(s)	586
BglII	AGATCT	1 cut(s)	267
BisI	GCNGC	5 cut(s)	312, 777, 780, 1077, 1188
BlsI	GCNGC	5 cut(s)	313, 778, 781, 1078, 1189
Bme1390I	CCNGG	4 cut(s)	241, 454, 1336, 1444
Bme18I	GGWCC	5 cut(s)	194, 326, 338, 821, 1538
BmgT120I	GGNCC	9 cut(s)	194, 326, 338, 531, 688, 821, 883, 1337, 1538
BmiI	GGNNCC	5 cut(s)	328, 339, 690, 1009, 1116
BmrFI	CCNGG	4 cut(s)	241, 454, 1336, 1444
BmsI	GCATC	7 cut(s)	10, 32, 356, 763, 852, 964, 1035
BpiI	GAAGAC	1 cut(s)	1405
BpmI	CTGGAG	1 cut(s)	1026
Bpu10I	CCTNAGC	1 cut(s)	693
BpuEI	CTTGAG	1 cut(s)	389
BpuMI	CCSGG	2 cut(s)	454, 1336
Bsa29I	ATCGAT	1 cut(s)	584
BsaAI	YACGTR	1 cut(s)	667
BsaI	GGTCTC	2 cut(s)	365, 1098
BsaJI	CCNNGG	2 cut(s)	1108, 1204
BsaWI	WCCGGW	1 cut(s)	152
BsaXI	ACNNNNNCTCC	2 cut(s)	936, 966
Bsc4I	CCNNNNNNNGG	6 cut(s)	170, 210, 211, 290, 335, 1389
Bse118I	RCCGGY	1 cut(s)	389
Bse1I	ACTGG	1 cut(s)	1009
Bse3DI	GCAATG	2 cut(s)	264, 384
BseBI	CCWGG	2 cut(s)	241, 1444
BseCI	ATCGAT	1 cut(s)	584
BseDI	CCNNGG	2 cut(s)	1108, 1204
BseGI	GGATG	3 cut(s)	45, 872, 1274
BseLI	CCNNNNNNNGG	6 cut(s)	170, 210, 211, 290, 335, 1389
BseMI	GCAATG	2 cut(s)	264, 384
BseMII	CTCAG	5 cut(s)	103, 416, 684, 783, 1308
BseNI	ACTGG	1 cut(s)	1009
BseRI	GAGGAG	2 cut(s)	819, 1409
BseXI	GCAGC	5 cut(s)	298, 763, 791, 1063, 1199
BsgI	GTGCAG	1 cut(s)	1206
BshFI	GGCC	5 cut(s)	516, 533, 689, 884, 1339
BshVI	ATCGAT	1 cut(s)	584
BsiSI	CCGG	4 cut(s)	153, 390, 453, 1335
BslFI	GGGAC	4 cut(s)	18, 312, 469, 622
BslI	CCNNNNNNNGG	6 cut(s)	170, 210, 211, 290, 335, 1389
BsmAI	GTCTC	3 cut(s)	365, 929, 1098
BsmFI	GGGAC	4 cut(s)	18, 312, 469, 622
BsmI	GAATGC	2 cut(s)	897, 1197
BsnI	GGCC	5 cut(s)	516, 533, 689, 884, 1339
Bso31I	GGTCTC	2 cut(s)	365, 1098
Bsp1286I	GDGCHC	1 cut(s)	384
Bsp1407I	TGTACA	1 cut(s)	507
Bsp143I	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
BspANI	GGCC	5 cut(s)	516, 533, 689, 884, 1339
BspCNI	CTCAG	5 cut(s)	102, 415, 685, 784, 1309
BspDI	ATCGAT	1 cut(s)	584
BspLI	GGNNCC	5 cut(s)	328, 339, 690, 1009, 1116
BspMAI	CTGCAG	2 cut(s)	781, 943
BspMI	ACCTGC	1 cut(s)	586
BspPI	GGATC	3 cut(s)	301, 424, 917
BspTNI	GGTCTC	2 cut(s)	365, 1098
BsrDI	GCAATG	2 cut(s)	264, 384
BsrFI	RCCGGY	1 cut(s)	389
BsrGI	TGTACA	1 cut(s)	507
BsrI	ACTGG	1 cut(s)	1009
BssAI	RCCGGY	1 cut(s)	389
BssECI	CCNNGG	2 cut(s)	1108, 1204
BssMI	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
BssT1I	CCWWGG	2 cut(s)	1108, 1204
Bst2UI	CCWGG	2 cut(s)	241, 1444
Bst4CI	ACNGT	2 cut(s)	654, 998
Bst6I	CTCTTC	2 cut(s)	88, 1129
BstAPI	GCANNNNNTGC	1 cut(s)	1469
BstAUI	TGTACA	1 cut(s)	507
BstBAI	YACGTR	1 cut(s)	667
BstC8I	GCNNGC	3 cut(s)	391, 716, 957
BstDEI	CTNAG	6 cut(s)	89, 319, 402, 693, 792, 1317
BstF5I	GGATG	3 cut(s)	45, 872, 1274
BstKTI	GATC	6 cut(s)	270, 296, 419, 925, 1296, 1564
BstMAI	GTCTC	3 cut(s)	365, 929, 1098
BstMBI	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
BstMWI	GCNNNNNNNGC	3 cut(s)	320, 1359, 1469
BstNI	CCWGG	2 cut(s)	241, 1444
BstNSI	RCATGY	1 cut(s)	508
BstSCI	CCNGG	4 cut(s)	239, 452, 1334, 1442
BstSFI	CTRYAG	3 cut(s)	288, 777, 939
BstV1I	GCAGC	5 cut(s)	298, 763, 791, 1063, 1199
BstV2I	GAAGAC	1 cut(s)	1405
BstX2I	RGATCY	3 cut(s)	267, 293, 416
BstXI	CCANNNNNNTGG	1 cut(s)	1443
BstYI	RGATCY	3 cut(s)	267, 293, 416
Bsu15I	ATCGAT	1 cut(s)	584
BsuRI	GGCC	5 cut(s)	516, 533, 689, 884, 1339
BsuTUI	ATCGAT	1 cut(s)	584
BtsCI	GGATG	3 cut(s)	45, 872, 1274
BtsI	GCAGTG	1 cut(s)	732
BtsIMutI	CAGTG	3 cut(s)	732, 864, 1002
BveI	ACCTGC	1 cut(s)	586
Cac8I	GCNNGC	3 cut(s)	391, 716, 957
Cfr10I	RCCGGY	1 cut(s)	389
Cfr13I	GGNCC	9 cut(s)	194, 326, 338, 531, 688, 821, 883, 1337, 1538
ClaI	ATCGAT	1 cut(s)	584
CseI	GACGC	1 cut(s)	648
CsiI	ACCWGGT	1 cut(s)	239
Csp6I	GTAC	3 cut(s)	399, 508, 1260
CviAII	CATG	3 cut(s)	351, 505, 983
CviQI	GTAC	3 cut(s)	399, 508, 1260
DdeI	CTNAG	6 cut(s)	89, 319, 402, 693, 792, 1317
DpnI	GATC	6 cut(s)	269, 295, 418, 924, 1295, 1563
DpnII	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
Eam1104I	CTCTTC	2 cut(s)	88, 1129
EarI	CTCTTC	2 cut(s)	88, 1129
Eco130I	CCWWGG	2 cut(s)	1108, 1204
Eco24I	GRGCYC	1 cut(s)	384
Eco31I	GGTCTC	2 cut(s)	365, 1098
Eco47I	GGWCC	5 cut(s)	194, 326, 338, 821, 1538
EcoO109I	RGGNCCY	3 cut(s)	194, 338, 1538
EcoRII	CCWGG	2 cut(s)	239, 1442
EcoT14I	CCWWGG	2 cut(s)	1108, 1204
EcoT22I	ATGCAT	4 cut(s)	25, 506, 979, 1028
EcoT38I	GRGCYC	1 cut(s)	384
ErhI	CCWWGG	2 cut(s)	1108, 1204
FaeI	CATG	3 cut(s)	354, 508, 986
FalI	AAGNNNNNCTT	2 cut(s)	703, 735
FaqI	GGGAC	4 cut(s)	18, 312, 469, 622
FatI	CATG	3 cut(s)	350, 504, 982
FauNDI	CATATG	3 cut(s)	187, 409, 1256
FbaI	TGATCA	1 cut(s)	1561
Fnu4HI	GCNGC	5 cut(s)	312, 777, 780, 1077, 1188
FokI	GGATG	3 cut(s)	52, 879, 1281
FriOI	GRGCYC	1 cut(s)	384
Fsp4HI	GCNGC	5 cut(s)	312, 777, 780, 1077, 1188
FspBI	CTAG	1 cut(s)	1071
GluI	GCNGC	5 cut(s)	312, 777, 780, 1077, 1188
GsuI	CTGGAG	1 cut(s)	1026
HaeIII	GGCC	5 cut(s)	516, 533, 689, 884, 1339
HapII	CCGG	4 cut(s)	153, 390, 453, 1335
HgaI	GACGC	1 cut(s)	648
Hin1II	CATG	3 cut(s)	354, 508, 986
HinfI	GANTC	3 cut(s)	44, 156, 730
HpaII	CCGG	4 cut(s)	153, 390, 453, 1335
HphI	GGTGA	2 cut(s)	142, 229
Hpy166II	GTNNAC	2 cut(s)	672, 1055
Hpy188I	TCNGA	3 cut(s)	92, 1293, 1494
Hpy188III	TCNNGA	3 cut(s)	133, 902, 1101
Hpy8I	GTNNAC	2 cut(s)	672, 1055
Hpy99I	CGWCG	1 cut(s)	139
HpyAV	CCTTC	3 cut(s)	577, 1173, 1393
HpyCH4III	ACNGT	2 cut(s)	654, 998
HpyCH4IV	ACGT	2 cut(s)	104, 666
HpyF10VI	GCNNNNNNNGC	3 cut(s)	320, 1359, 1469
HpyF3I	CTNAG	6 cut(s)	89, 319, 402, 693, 792, 1317
HpySE526I	ACGT	2 cut(s)	104, 666
Hsp92II	CATG	3 cut(s)	354, 508, 986
KroI	GCCGGC	1 cut(s)	389
KroNI	GCCGGC	1 cut(s)	391
Ksp22I	TGATCA	1 cut(s)	1561
Kzo9I	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
LmnI	GCTCC	3 cut(s)	806, 944, 1007
Lsp1109I	GCAGC	5 cut(s)	298, 763, 791, 1063, 1199
LweI	GCATC	7 cut(s)	10, 32, 356, 763, 852, 964, 1035
MabI	ACCWGGT	1 cut(s)	239
MaeI	CTAG	1 cut(s)	1071
MaeII	ACGT	2 cut(s)	104, 666
MaeIII	GTNAC	2 cut(s)	74, 1397
MalI	GATC	6 cut(s)	269, 295, 418, 924, 1295, 1563
MboI	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
MboII	GAAGA	5 cut(s)	105, 1036, 1116, 1333, 1405
MflI	RGATCY	3 cut(s)	267, 293, 416
MhlI	GDGCHC	1 cut(s)	384
MluCI	AATT	5 cut(s)	612, 963, 1062, 1437, 1456
MmeI	TCCRAC	1 cut(s)	583
Mph1103I	ATGCAT	4 cut(s)	25, 506, 979, 1028
MroNI	GCCGGC	1 cut(s)	389
MroXI	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
MseI	TTAA	3 cut(s)	63, 1220, 1514
MslI	CAYNNNNRTG	3 cut(s)	352, 487, 857
MspI	CCGG	4 cut(s)	153, 390, 453, 1335
MspR9I	CCNGG	4 cut(s)	241, 454, 1336, 1444
Mva1269I	GAATGC	2 cut(s)	897, 1197
MvaI	CCWGG	2 cut(s)	241, 1444
MwoI	GCNNNNNNNGC	3 cut(s)	320, 1359, 1469
NaeI	GCCGGC	1 cut(s)	391
NciI	CCSGG	2 cut(s)	454, 1336
NdeI	CATATG	3 cut(s)	187, 409, 1256
NdeII	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
NgoMIV	GCCGGC	1 cut(s)	389
NlaIII	CATG	3 cut(s)	354, 508, 986
NlaIV	GGNNCC	5 cut(s)	328, 339, 690, 1009, 1116
NsiI	ATGCAT	4 cut(s)	25, 506, 979, 1028
NspI	RCATGY	1 cut(s)	508
OliI	CACNNNNGTG	1 cut(s)	487
PctI	GAATGC	2 cut(s)	897, 1197
PdiI	GCCGGC	1 cut(s)	391
PdmI	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
PfeI	GAWTC	3 cut(s)	44, 156, 730
PfoI	TCCNGGA	1 cut(s)	1442
PkrI	GCNGC	5 cut(s)	313, 778, 781, 1078, 1189
Ppu21I	YACGTR	1 cut(s)	667
PpuMI	RGGWCCY	3 cut(s)	194, 338, 1538
Psp5II	RGGWCCY	3 cut(s)	194, 338, 1538
Psp6I	CCWGG	2 cut(s)	239, 1442
PspGI	CCWGG	2 cut(s)	239, 1442
PspN4I	GGNNCC	5 cut(s)	328, 339, 690, 1009, 1116
PspPI	GGNCC	9 cut(s)	194, 326, 338, 531, 688, 821, 883, 1337, 1538
PspPPI	RGGWCCY	3 cut(s)	194, 338, 1538
PstI	CTGCAG	2 cut(s)	781, 943
PsuI	RGATCY	3 cut(s)	267, 293, 416
RsaI	GTAC	3 cut(s)	400, 509, 1261
RsaNI	GTAC	3 cut(s)	399, 508, 1260
RseI	CAYNNNNRTG	3 cut(s)	352, 487, 857
SaqAI	TTAA	3 cut(s)	63, 1220, 1514
SatI	GCNGC	5 cut(s)	312, 777, 780, 1077, 1188
Sau3AI	GATC	6 cut(s)	267, 293, 416, 922, 1293, 1561
Sau96I	GGNCC	9 cut(s)	194, 326, 338, 531, 688, 821, 883, 1337, 1538
ScrFI	CCNGG	4 cut(s)	241, 454, 1336, 1444
SduI	GDGCHC	1 cut(s)	384
SexAI	ACCWGGT	1 cut(s)	239
SfaNI	GCATC	7 cut(s)	10, 32, 356, 763, 852, 964, 1035
SfcI	CTRYAG	3 cut(s)	288, 777, 939
SinI	GGWCC	5 cut(s)	194, 326, 338, 821, 1538
SmiMI	CAYNNNNRTG	3 cut(s)	352, 487, 857
SmlI	CTYRAG	1 cut(s)	368
SmoI	CTYRAG	1 cut(s)	368
SpeI	ACTAGT	1 cut(s)	1070
Sse9I	AATT	5 cut(s)	612, 963, 1062, 1437, 1456
SspMI	CTAG	1 cut(s)	1071
StyD4I	CCNGG	4 cut(s)	239, 452, 1334, 1442
StyI	CCWWGG	2 cut(s)	1108, 1204
TaaI	ACNGT	2 cut(s)	654, 998
TaiI	ACGT	2 cut(s)	107, 669
TaqI	TCGA	4 cut(s)	134, 584, 681, 1554
TasI	AATT	5 cut(s)	612, 963, 1062, 1437, 1456
TatI	WGTACW	1 cut(s)	507
TfiI	GAWTC	3 cut(s)	44, 156, 730
Tru1I	TTAA	3 cut(s)	63, 1220, 1514
Tru9I	TTAA	3 cut(s)	63, 1220, 1514
TscAI	CASTG	3 cut(s)	732, 864, 1009
TseI	GCWGC	5 cut(s)	311, 776, 779, 1076, 1187
TspDTI	ATGAA	5 cut(s)	264, 559, 747, 971, 1029
TspGWI	ACGGA	1 cut(s)	1117
TspRI	CASTG	3 cut(s)	732, 864, 1009
VpaK11BI	GGWCC	5 cut(s)	194, 326, 338, 821, 1538
XapI	RAATTY	1 cut(s)	963
XceI	RCATGY	1 cut(s)	508
XmnI	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
XspI	CTAG	1 cut(s)	1071
Zsp2I	ATGCAT	4 cut(s)	25, 506, 979, 1028

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.