Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001248

Physical Location & Seq

Genomic Coordinates Reverse (-)

19860 .. 23276

Gene Structure 3417 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1000200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGGTGCACAACGAAGTAGATGCGTCACGGATACAAGAGATGGATTCAGGGGAAAGGCGCCTTAACGAGCTTGGTTACAAGCAAGAACTCAGACGAGAGATGACTTTGTTCAAAACTCTGGCAATTTCATTCTCGACAATGACACTTTTCACCGGAATTACTCCCTTGTATGGCTCAAGCCTTTTATATGCAGGTCCTGCAAGCCTTGTGTGGGGATGGGTGGTCGTTTCATTTTTCACTTGCTTTGTAGGAGTTGCCATGGCAGAGATCTGCTCCTCTTTCCCGACTACAGGTTCTCTTTACTTCTGGGCTGCCCATTTAGCCGGCCCCAAATGGGGTCCATTTGCCTCATGGTGCTGCGCTTGGCTTGAGACGGTAGGCCTCATTGCCGGCATAGGCACTCAGGCATATGCAGGATCGCAAACGCTGCAAAGTATCATTTTGTTATCCACTGGGACAAATAAAGATGGAGGATATCTTGCGCCAAAATGGCTATTCTTATGCATGTACATTGGACTCACTGTTATATGGGCGTTTCTCAACACATTTGCGCTAGAAGTAATTGCCTTTATTGACATAATCTCGATATGGTGGCAGGTCATTGGAGGGCTGGTTATTGTGATAACACTTCCCCTGGTAGCAATGACGACACAGTCAGCATCATATGTGTTCACAAATTTCGAAGTGGCTTCTGAGCAGACTGGGATATCAAGCAAACCTTATGCAGTGATTCTATCCTTTCTTGTTAGTCAGTATTCATTATATGGATATGATGCTGCAGCACATCTAACTGAAGAAACCAAGGGAGCAGACAAGAACGGTCCTATTGCTATTCTATCTAGCATTGCTATCATATCAGTGTTTGGGTGGGCATACATCTTGGCTCTTACTTTCAGCATTCAGGACTTTAGCTACCTATATGATCCAACCAACGAGACTGCTGGAACATTTGTGCCAGCTCAAATACTCTATGATGCATTTCATGGGAGGTATCAGAGTTCCACAGGAGCAATCATTTTACTATGCATCATTTGGGCTTCATTCTTCTTCGGTGGGCTTTCAATTACCACTAGTGCTGCCAGAGTGGTATATGCTCTATCACGAGACCAAGGTATTCCATTTTCGTCTGTATGGAAAAAATTGCACCCGAAACACAAAGTTCCTTCAAATGCAGTGTGGCTCTGTGCAGCGATCTGCATTCTCCTCGGACTTCCAATCTTGAAAGTCAATGTAGTCTTCACTGCCATAACTTCGATATGTACAATTGGATGGGTAGGAGGATATGCAGTTCCAATCTTTGCAAGATTGGTAATGCCTGAGAAGAACTTCAACGCTGGACCATTTTACTTGGGGAAAGCTAGAAGGCCAATTTGCTTGGTTGCCTTCCTGTGGATTTGTTATACCTGTTCGGTCTTCCTCCTCCCGACTTTCTACCCAATTACTTGGAATACATTCAATTATGCACCTGTTGCTTTGAGTGTGGGATTGGGTCTAGTAATGCTTTGGTGGATGTTGGATGCAAGGAAATGGTTCAAAGGACCTGTTAGAAACATTGAAACTGAAAACGGAAAGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015179 GO:0015291 GO:0015297 GO:0015318 GO:0015711 GO:0015807 GO:0015849 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1902475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2

Protein Analysis

526

Amino Acids

57.88

Weight (kDa)

8.16

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	35 - 491	3.1e-56	Amino acid permease
AA_permease	PF00324	58 - 507	6.4e-21	Amino acid permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001248)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g24970 FvH4_2g27760 FvH4_2g27770 FvH4_2g27771
malus_domestica	MD00G1162300.v1.1 MD01G1000200.v1.1
prunus_persica	Prupe.1G490100_v2.0.a1 Prupe.1G490200_v2.0.a1 Prupe.1G490200_v2.0.a1 Prupe.1G490300_v2.0.a1 Prupe.4G276400_v2.0.a1 Prupe.4G276400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0122601 RchiOBHm_Chr6g0296631 RchiOBHm_Chr6g0296641 RchiOBHm_Chr6g0296651
rosa_laevigata	RLG00000011673 RLG00000011674 RLG00000011675 RLG00000018694
rosa_multiflora	Rmu_sc0001741.1_g000017 Rmu_sc0001741.1_g000018 Rmu_sc0003133.1_g000008
rosa_roxburghii	Rroxscaffold_7G00171300 Rroxscaffold_7G00171310 Rroxscaffold_7G00171320 Rroxscaffold_7G00178780 Rroxscaffold_7G00178790
rosa_rugosa	Rorug02G0240000 Rorug06G0263800 Rorug06G0263900 Rorug06G0263900
rosa_samantha	Rh2AG298500 Rh2BG307300 Rh2CG285400 Rh2DG322100 Rh6AG375200 Rh6AG375300 Rh6AG375400 Rh6AG375500 Rh6BG383700 Rh6BG383800 Rh6BG383900 Rh6CG388900 Rh6CG389000 Rh6CG389100 Rh6DG376200 Rh6DG376300 Rh6DG376400
rosa_wichuraiana	Rw2G023730 Rw6G032730 Rw6G032740 Rw6G032750 Rw6G032820 Rw6G032830 Rw6G032840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	652
Acc36I	ACCTGC	2 cut(s)	182, 586
AccB1I	GGYRCC	1 cut(s)	57
AclWI	GGATC	2 cut(s)	424, 917
AcsI	RAATTY	1 cut(s)	676
AcuI	CTGAAG	1 cut(s)	813
AcyI	GRCGYC	1 cut(s)	58
AfaI	GTAC	2 cut(s)	509, 1261
AfiI	CCNNNNNNNGG	6 cut(s)	170, 211, 290, 334, 335, 1389
AgsI	TTSAA	8 cut(s)	112, 1062, 1167, 1222, 1330, 1456, 1534, 1556
AhlI	ACTAGT	1 cut(s)	1070
AjnI	CCWGG	1 cut(s)	633
AloI	GAACNNNNNNTCC	2 cut(s)	1272, 1304
AluBI	AGCT	4 cut(s)	70, 912, 959, 1358
AluI	AGCT	4 cut(s)	70, 912, 959, 1358
Alw21I	GWGCWC	1 cut(s)	9
Alw26I	GTCTC	3 cut(s)	365, 929, 1098
Alw44I	GTGCAC	1 cut(s)	5
AlwI	GGATC	2 cut(s)	424, 917
AlwNI	CAGNNNCTG	1 cut(s)	197
AoxI	GGCC	3 cut(s)	325, 379, 1364
ApaLI	GTGCAC	1 cut(s)	5
ApeKI	GCWGC	7 cut(s)	311, 357, 427, 776, 779, 1076, 1187
ApoI	RAATTY	1 cut(s)	676
Asp700I	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
AspLEI	GCGC	4 cut(s)	60, 362, 484, 553
AspS9I	GGNCC	6 cut(s)	194, 326, 338, 821, 1337, 1538
AsuHPI	GGTGA	1 cut(s)	142
AsuII	TTCGAA	1 cut(s)	681
AvaII	GGWCC	5 cut(s)	194, 338, 821, 1337, 1538
BaeGI	GKGCMC	1 cut(s)	9
BanI	GGYRCC	1 cut(s)	57
BauI	CACGAG	1 cut(s)	1101
BbsI	GAAGAC	2 cut(s)	1228, 1405
Bbv12I	GWGCWC	1 cut(s)	9
BbvI	GCAGC	7 cut(s)	298, 344, 414, 763, 791, 1063, 1199
BccI	CCATC	4 cut(s)	34, 210, 461, 1263
BcgI	CGANNNNNNTGC	3 cut(s)	36, 1186, 1220
BciT130I	CCWGG	1 cut(s)	635
BciVI	GTATCC	1 cut(s)	24
BcoDI	GTCTC	3 cut(s)	365, 929, 1098
BcuI	ACTAGT	1 cut(s)	1070
BfaI	CTAG	5 cut(s)	554, 840, 1071, 1359, 1493
BfmI	CTRYAG	2 cut(s)	288, 777
BfoI	RGCGCY	1 cut(s)	61
BfuAI	ACCTGC	2 cut(s)	182, 586
BfuI	GTATCC	1 cut(s)	24
BglI	GCCNNNNNGGC	1 cut(s)	490
BglII	AGATCT	1 cut(s)	267
BisI	GCNGC	7 cut(s)	312, 358, 428, 777, 780, 1077, 1188
BlsI	GCNGC	7 cut(s)	313, 359, 429, 778, 781, 1078, 1189
Bme1390I	CCNGG	1 cut(s)	635
Bme18I	GGWCC	5 cut(s)	194, 338, 821, 1337, 1538
BmgT120I	GGNCC	6 cut(s)	194, 326, 338, 821, 1337, 1538
BmiI	GGNNCC	3 cut(s)	59, 328, 339
BmrFI	CCNGG	1 cut(s)	635
BmrI	ACTGGG	2 cut(s)	462, 711
BmsI	GCATC	6 cut(s)	10, 668, 763, 964, 1035, 1507
BmuI	ACTGGG	2 cut(s)	462, 711
BpiI	GAAGAC	2 cut(s)	1228, 1405
BplI	GAGNNNNNCTC	2 cut(s)	257, 289
Bpu14I	TTCGAA	1 cut(s)	681
BpuEI	CTTGAG	2 cut(s)	160, 389
BsaHI	GRCGYC	1 cut(s)	58
BsaI	GGTCTC	1 cut(s)	1098
BsaJI	CCNNGG	5 cut(s)	258, 633, 801, 1108, 1204
BsaWI	WCCGGW	1 cut(s)	152
BsaXI	ACNNNNNCTCC	2 cut(s)	597, 627
Bsc4I	CCNNNNNNNGG	6 cut(s)	170, 211, 290, 334, 335, 1389
Bse118I	RCCGGY	2 cut(s)	323, 389
Bse1I	ACTGG	2 cut(s)	457, 706
Bse3DI	GCAATG	3 cut(s)	384, 648, 843
BseBI	CCWGG	1 cut(s)	635
BseDI	CCNNGG	5 cut(s)	258, 633, 801, 1108, 1204
BseGI	GGATG	4 cut(s)	221, 1274, 1515, 1522
BseLI	CCNNNNNNNGG	6 cut(s)	170, 211, 290, 334, 335, 1389
BseMI	GCAATG	3 cut(s)	384, 648, 843
BseMII	CTCAG	4 cut(s)	103, 416, 684, 1308
BseNI	ACTGG	2 cut(s)	457, 706
BseRI	GAGGAG	3 cut(s)	265, 1193, 1409
BseSI	GKGCMC	1 cut(s)	9
BseXI	GCAGC	7 cut(s)	298, 344, 414, 763, 791, 1063, 1199
BsgI	GTGCAG	1 cut(s)	1206
BshFI	GGCC	3 cut(s)	327, 381, 1366
BshNI	GGYRCC	1 cut(s)	57
BsiHKAI	GWGCWC	1 cut(s)	9
BsiSI	CCGG	3 cut(s)	153, 324, 390
BslFI	GGGAC	1 cut(s)	469
BslI	CCNNNNNNNGG	6 cut(s)	170, 211, 290, 334, 335, 1389
BsmAI	GTCTC	3 cut(s)	365, 929, 1098
BsmBI	CGTCTC	1 cut(s)	365
BsmFI	GGGAC	1 cut(s)	469
BsmI	GAATGC	2 cut(s)	897, 1197
BsnI	GGCC	3 cut(s)	327, 381, 1366
Bso31I	GGTCTC	1 cut(s)	1098
Bsp119I	TTCGAA	1 cut(s)	681
Bsp1286I	GDGCHC	1 cut(s)	9
Bsp1407I	TGTACA	2 cut(s)	507, 1259
Bsp143I	GATC	4 cut(s)	267, 416, 922, 1191
Bsp19I	CCATGG	1 cut(s)	258
BspANI	GGCC	3 cut(s)	327, 381, 1366
BspCNI	CTCAG	4 cut(s)	102, 415, 685, 1309
BspLI	GGNNCC	3 cut(s)	59, 328, 339
BspMAI	CTGCAG	1 cut(s)	781
BspMI	ACCTGC	2 cut(s)	182, 586
BspPI	GGATC	2 cut(s)	424, 917
BspT104I	TTCGAA	1 cut(s)	681
BspT107I	GGYRCC	1 cut(s)	57
BspTNI	GGTCTC	1 cut(s)	1098
BsrDI	GCAATG	3 cut(s)	384, 648, 843
BsrFI	RCCGGY	2 cut(s)	323, 389
BsrGI	TGTACA	2 cut(s)	507, 1259
BsrI	ACTGG	2 cut(s)	457, 706
BssAI	RCCGGY	2 cut(s)	323, 389
BssECI	CCNNGG	5 cut(s)	258, 633, 801, 1108, 1204
BssMI	GATC	4 cut(s)	267, 416, 922, 1191
BssNI	GRCGYC	1 cut(s)	58
BssSI	CACGAG	1 cut(s)	1101
BssT1I	CCWWGG	3 cut(s)	258, 801, 1108
Bst2BI	CACGAG	1 cut(s)	1101
Bst2UI	CCWGG	1 cut(s)	635
Bst4CI	ACNGT	4 cut(s)	376, 523, 654, 821
BstACI	GRCGYC	1 cut(s)	58
BstAPI	GCANNNNNTGC	3 cut(s)	197, 427, 1469
BstAUI	TGTACA	2 cut(s)	507, 1259
BstBI	TTCGAA	1 cut(s)	681
BstC8I	GCNNGC	4 cut(s)	202, 325, 391, 957
BstDEI	CTNAG	4 cut(s)	89, 402, 693, 1317
BstDSI	CCRYGG	1 cut(s)	258
BstF5I	GGATG	4 cut(s)	221, 1274, 1515, 1522
BstH2I	RGCGCY	1 cut(s)	61
BstHHI	GCGC	4 cut(s)	60, 362, 484, 553
BstKTI	GATC	4 cut(s)	270, 419, 925, 1194
BstMAI	GTCTC	3 cut(s)	365, 929, 1098
BstMBI	GATC	4 cut(s)	267, 416, 922, 1191
BstMWI	GCNNNNNNNGC	5 cut(s)	197, 320, 427, 490, 1469
BstNI	CCWGG	1 cut(s)	635
BstNSI	RCATGY	1 cut(s)	508
BstSCI	CCNGG	1 cut(s)	633
BstSFI	CTRYAG	2 cut(s)	288, 777
BstSLI	GKGCMC	1 cut(s)	9
BstV1I	GCAGC	7 cut(s)	298, 344, 414, 763, 791, 1063, 1199
BstV2I	GAAGAC	2 cut(s)	1228, 1405
BstX2I	RGATCY	1 cut(s)	267
BstXI	CCANNNNNNTGG	1 cut(s)	1443
BstYI	RGATCY	1 cut(s)	267
BsuI	GTATCC	1 cut(s)	24
BsuRI	GGCC	3 cut(s)	327, 381, 1366
BtgI	CCRYGG	1 cut(s)	258
BtsCI	GGATG	4 cut(s)	221, 1274, 1515, 1522
BtsI	GCAGTG	3 cut(s)	732, 1179, 1239
BtsIMutI	CAGTG	6 cut(s)	450, 519, 732, 864, 1179, 1239
BveI	ACCTGC	2 cut(s)	182, 586
Cac8I	GCNNGC	4 cut(s)	202, 325, 391, 957
CaiI	CAGNNNCTG	1 cut(s)	197
CfoI	GCGC	4 cut(s)	60, 362, 484, 553
Cfr10I	RCCGGY	2 cut(s)	323, 389
Cfr13I	GGNCC	6 cut(s)	194, 326, 338, 821, 1337, 1538
CseI	GACGC	1 cut(s)	12
Csp6I	GTAC	2 cut(s)	508, 1260
CviAII	CATG	4 cut(s)	259, 351, 505, 983
CviQI	GTAC	2 cut(s)	508, 1260
DdeI	CTNAG	4 cut(s)	89, 402, 693, 1317
DinI	GGCGCC	1 cut(s)	59
DpnI	GATC	4 cut(s)	269, 418, 924, 1193
DpnII	GATC	4 cut(s)	267, 416, 922, 1191
DrdI	GACNNNNNNGTC	1 cut(s)	652
DseDI	GACNNNNNNGTC	1 cut(s)	652
Eco130I	CCWWGG	3 cut(s)	258, 801, 1108
Eco147I	AGGCCT	1 cut(s)	381
Eco31I	GGTCTC	1 cut(s)	1098
Eco32I	GATATC	2 cut(s)	476, 708
Eco47I	GGWCC	5 cut(s)	194, 338, 821, 1337, 1538
Eco57I	CTGAAG	1 cut(s)	813
EcoO109I	RGGNCCY	2 cut(s)	194, 1538
EcoRII	CCWGG	1 cut(s)	633
EcoRV	GATATC	2 cut(s)	476, 708
EcoT14I	CCWWGG	3 cut(s)	258, 801, 1108
EcoT22I	ATGCAT	3 cut(s)	506, 979, 1028
EgeI	GGCGCC	1 cut(s)	59
EheI	GGCGCC	1 cut(s)	59
ErhI	CCWWGG	3 cut(s)	258, 801, 1108
Esp3I	CGTCTC	1 cut(s)	365
FaeI	CATG	4 cut(s)	262, 354, 508, 986
FalI	AAGNNNNNCTT	2 cut(s)	703, 735
FaqI	GGGAC	1 cut(s)	469
FatI	CATG	4 cut(s)	258, 350, 504, 982
FauNDI	CATATG	2 cut(s)	409, 664
Fnu4HI	GCNGC	7 cut(s)	312, 358, 428, 777, 780, 1077, 1188
FokI	GGATG	4 cut(s)	228, 1281, 1522, 1529
Fsp4HI	GCNGC	7 cut(s)	312, 358, 428, 777, 780, 1077, 1188
FspBI	CTAG	5 cut(s)	554, 840, 1071, 1359, 1493
GlaI	GCGC	4 cut(s)	59, 361, 483, 552
GluI	GCNGC	7 cut(s)	312, 358, 428, 777, 780, 1077, 1188
HaeII	RGCGCY	1 cut(s)	61
HaeIII	GGCC	3 cut(s)	327, 381, 1366
HapII	CCGG	3 cut(s)	153, 324, 390
HgaI	GACGC	1 cut(s)	12
HhaI	GCGC	4 cut(s)	60, 362, 484, 553
Hin1I	GRCGYC	1 cut(s)	58
Hin1II	CATG	4 cut(s)	262, 354, 508, 986
Hin6I	GCGC	4 cut(s)	58, 360, 482, 551
HinP1I	GCGC	4 cut(s)	58, 360, 482, 551
HinfI	GANTC	3 cut(s)	44, 516, 730
HpaII	CCGG	3 cut(s)	153, 324, 390
HphI	GGTGA	1 cut(s)	142
Hpy166II	GTNNAC	2 cut(s)	7, 672
Hpy188I	TCNGA	4 cut(s)	92, 694, 996, 1208
Hpy188III	TCNNGA	7 cut(s)	133, 283, 583, 902, 1101, 1219, 1423
Hpy8I	GTNNAC	2 cut(s)	7, 672
HpyAV	CCTTC	3 cut(s)	1173, 1356, 1393
HpyCH4III	ACNGT	4 cut(s)	376, 523, 654, 821
HpyF10VI	GCNNNNNNNGC	5 cut(s)	197, 320, 427, 490, 1469
HpyF3I	CTNAG	4 cut(s)	89, 402, 693, 1317
Hsp92I	GRCGYC	1 cut(s)	58
Hsp92II	CATG	4 cut(s)	262, 354, 508, 986
HspAI	GCGC	4 cut(s)	58, 360, 482, 551
KasI	GGCGCC	1 cut(s)	57
KroI	GCCGGC	2 cut(s)	323, 389
KroNI	GCCGGC	2 cut(s)	325, 391
Kzo9I	GATC	4 cut(s)	267, 416, 922, 1191
LmnI	GCTCC	3 cut(s)	278, 806, 1007
Lsp1109I	GCAGC	7 cut(s)	298, 344, 414, 763, 791, 1063, 1199
LweI	GCATC	6 cut(s)	10, 668, 763, 964, 1035, 1507
MaeI	CTAG	5 cut(s)	554, 840, 1071, 1359, 1493
MaeIII	GTNAC	2 cut(s)	24, 74
MalI	GATC	4 cut(s)	269, 418, 924, 1193
MboI	GATC	4 cut(s)	267, 416, 922, 1191
MboII	GAAGA	6 cut(s)	806, 1036, 1039, 1228, 1333, 1405
MfeI	CAATTG	1 cut(s)	1263
MflI	RGATCY	1 cut(s)	267
MhlI	GDGCHC	1 cut(s)	9
Mly113I	GGCGCC	1 cut(s)	58
MlyI	GAGTC	1 cut(s)	510
MmeI	TCCRAC	2 cut(s)	950, 1494
Mph1103I	ATGCAT	3 cut(s)	506, 979, 1028
MroNI	GCCGGC	2 cut(s)	323, 389
MroXI	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
MseI	TTAA	1 cut(s)	63
MslI	CAYNNNNRTG	1 cut(s)	857
MspI	CCGG	3 cut(s)	153, 324, 390
MspR9I	CCNGG	1 cut(s)	635
MunI	CAATTG	1 cut(s)	1263
Mva1269I	GAATGC	2 cut(s)	897, 1197
MvaI	CCWGG	1 cut(s)	635
MwoI	GCNNNNNNNGC	5 cut(s)	197, 320, 427, 490, 1469
NaeI	GCCGGC	2 cut(s)	325, 391
NarI	GGCGCC	1 cut(s)	58
NcoI	CCATGG	1 cut(s)	258
NdeI	CATATG	2 cut(s)	409, 664
NdeII	GATC	4 cut(s)	267, 416, 922, 1191
NgoMIV	GCCGGC	2 cut(s)	323, 389
NlaIII	CATG	4 cut(s)	262, 354, 508, 986
NlaIV	GGNNCC	3 cut(s)	59, 328, 339
NmuCI	GTSAC	1 cut(s)	24
NsiI	ATGCAT	3 cut(s)	506, 979, 1028
NspI	RCATGY	1 cut(s)	508
NspV	TTCGAA	1 cut(s)	681
PceI	AGGCCT	1 cut(s)	381
PctI	GAATGC	2 cut(s)	897, 1197
PdiI	GCCGGC	2 cut(s)	325, 391
PdmI	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
PfeI	GAWTC	2 cut(s)	44, 730
PflFI	GACNNNGTC	1 cut(s)	652
PkrI	GCNGC	7 cut(s)	313, 359, 429, 778, 781, 1078, 1189
PleI	GAGTC	1 cut(s)	510
PluTI	GGCGCC	1 cut(s)	61
PpsI	GAGTC	1 cut(s)	510
PpuMI	RGGWCCY	2 cut(s)	194, 1538
Psp5II	RGGWCCY	2 cut(s)	194, 1538
Psp6I	CCWGG	1 cut(s)	633
PspGI	CCWGG	1 cut(s)	633
PspN4I	GGNNCC	3 cut(s)	59, 328, 339
PspPI	GGNCC	6 cut(s)	194, 326, 338, 821, 1337, 1538
PspPPI	RGGWCCY	2 cut(s)	194, 1538
PstI	CTGCAG	1 cut(s)	781
PstNI	CAGNNNCTG	1 cut(s)	197
PsuI	RGATCY	1 cut(s)	267
PsyI	GACNNNGTC	1 cut(s)	652
RsaI	GTAC	2 cut(s)	509, 1261
RsaNI	GTAC	2 cut(s)	508, 1260
RseI	CAYNNNNRTG	1 cut(s)	857
SaqAI	TTAA	1 cut(s)	63
SatI	GCNGC	7 cut(s)	312, 358, 428, 777, 780, 1077, 1188
Sau3AI	GATC	4 cut(s)	267, 416, 922, 1191
Sau96I	GGNCC	6 cut(s)	194, 326, 338, 821, 1337, 1538
SchI	GAGTC	1 cut(s)	510
ScrFI	CCNGG	1 cut(s)	635
SduI	GDGCHC	1 cut(s)	9
SfaNI	GCATC	6 cut(s)	10, 668, 763, 964, 1035, 1507
SfcI	CTRYAG	2 cut(s)	288, 777
SfoI	GGCGCC	1 cut(s)	59
SfuI	TTCGAA	1 cut(s)	681
SinI	GGWCC	5 cut(s)	194, 338, 821, 1337, 1538
SmiMI	CAYNNNNRTG	1 cut(s)	857
SmlI	CTYRAG	2 cut(s)	175, 368
SmoI	CTYRAG	2 cut(s)	175, 368
SpeI	ACTAGT	1 cut(s)	1070
SseBI	AGGCCT	1 cut(s)	381
SspDI	GGCGCC	1 cut(s)	57
SspMI	CTAG	5 cut(s)	554, 840, 1071, 1359, 1493
StuI	AGGCCT	1 cut(s)	381
StyD4I	CCNGG	1 cut(s)	633
StyI	CCWWGG	3 cut(s)	258, 801, 1108
TaaI	ACNGT	4 cut(s)	376, 523, 654, 821
TaqI	TCGA	4 cut(s)	134, 584, 681, 1253
TaqII	GACCGA	1 cut(s)	1399
TatI	WGTACW	2 cut(s)	507, 1259
TfiI	GAWTC	2 cut(s)	44, 730
Tru1I	TTAA	1 cut(s)	63
Tru9I	TTAA	1 cut(s)	63
TscAI	CASTG	6 cut(s)	457, 526, 732, 864, 1179, 1246
TseFI	GTSAC	1 cut(s)	24
TseI	GCWGC	7 cut(s)	311, 357, 427, 776, 779, 1076, 1187
Tsp45I	GTSAC	1 cut(s)	24
TspDTI	ATGAA	5 cut(s)	117, 219, 747, 971, 1029
TspGWI	ACGGA	1 cut(s)	43
TspRI	CASTG	6 cut(s)	457, 526, 732, 864, 1179, 1246
Tth111I	GACNNNGTC	1 cut(s)	652
VneI	GTGCAC	1 cut(s)	5
VpaK11BI	GGWCC	5 cut(s)	194, 338, 821, 1337, 1538
XapI	RAATTY	1 cut(s)	676
XceI	RCATGY	1 cut(s)	508
XcmI	CCANNNNNNNNNTGG	1 cut(s)	348
XmnI	GAANNNNTTC	3 cut(s)	1325, 1451, 1529
XspI	CTAG	5 cut(s)	554, 840, 1071, 1359, 1493
Zsp2I	ATGCAT	3 cut(s)	506, 979, 1028

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.