Rw6G032730

Amino-acid permease BAT1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001248
Physical Location & Seq
Reverse (-)
56989903 .. 56992311
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032730.1

Sequence Viewer

Length: 1452 bp
ATGGCTCAATATGTTTTGGAAATGGATTCTGGAGAAAAGCGTCTCAATGAGCTTGGTTACAAGCAAGAACTCAGAAGGGAAATGACGTTGTTCAAGACACTTGCAATAACATTTTCATGCATGTCAGTGTTCACTGGTACACCTCTCTATGGCCAAAGCTTGCGTTATGCAGGTCCTGCAACTTTAATATGGGGATGGTTAGTTGTCACCTTCTTCACATGGTTTGTCGGAATAGCCATGGCTGAAATTTGTTCTTCTTTCCCGACCACAGGTTCTCTTTATTTCTGGGCAGCCCATTTGGCTGGACCCAGGTGGGGCCCATTTGCTTCATGGTGCTGTGCTTGGCTTGAGATAATTGGTCTGACAACTGCAATAGGAGCTCAGGCATATTCAGCAACACAAGCATTTCAGATGTTCATTCTTATAGCCACTGGGACAAACAAGGGTGGAGGCTATTTTGCTTCAAGGAGTGTCTTTGTGTGCATGTATATGGGCATAACCCTCATTTGGGCAGTACTAAACACTTTTGCTCTGCAATTCATAGCATTGCTTAACATAATCTCCATATGGTGGCAGGTGATTGGTGGTTTGCTTGTGATTATAATGCTACCTCTGGTGGCACCGTCAACACAAACAGCTTCTTCTGTCTTCACTCATTTTGAAACATCTCCCGAGTCGACTGGGATATCTAGCGTACCTTATGCAGTGGTTTTGTCAGTGCTTCTGAGCAATTACTGTCTAGGTGCAGATAAAACTGGACCTATAGCTATTCTCTCCAGTATTGGGATAATATCAGTGTTTGGTTGGGCTTATTACTTAGCTCTCACTTTCAGCATCCAGGATCTGGACTATTTATACAGTGCAGACAATGAGACTGCTGGTGTACTTGTACCAGCACAGATAATATATGATGCATTTCATGGAAGGTATCATAATTCCACTGGAGCTGTGATTTTCCTGTTTATTATCTGGGGTTCCTTCTTCTTTTGTGGGCTGTCTGTCACCACTACTGCTGCCCGAGTAGTTTATGCTGTATCAAGGGATGGATGTATGCCTTTTTCACCAGTGTGGAGAAAAGTAAACCCGAGGAACAAGGTTCCAACAAATGCTGTCACCATTGGCTGGGTGGGAGGTTATGCTGTACCAATCTTTGCTAGGCTGGTGATGGCTGAAGAGAACTTCAAACCAGGACCCTATTATTTGGGTAGAGCAAGGCGGCCAGTTTGCTTGGTGGCTTTCCTCTGGATATGTTATACCTGTTCAGTCTTCCTCTTGCCGACTTCTTACCCTATTCGATGGAAAACTTTCAATTATGCACCAGTGGCCCTGGGTGTTGTTTTGACACTAGTGATGATCTGGTGGGCGTTGGATGCTAGGAAATGGTACAAAGGACCTGTAAGAAACATTGATGTACAAAATGGAAACAATCAGCATCCGCAGTCACACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2

Protein Analysis

483

Amino Acids

53.64

Weight (kDa)

8.86

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 30 - 384 1.7e-32 Amino acid permease
AA_permease PF00324 51 - 382 2.5e-11 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AarI CACCTGC 1 cut(s) 565
Acc36I ACCTGC 2 cut(s) 161, 565
AccB1I GGYRCC 1 cut(s) 619
AccB7I CCANNNNNTGG 3 cut(s) 570, 844, 1122
AccI GTMKAC 1 cut(s) 677
AciI CCGC 2 cut(s) 1216, 1436
AclWI GGATC 1 cut(s) 849
AcoI YGGCCR 2 cut(s) 151, 1217
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 1191
AfaI GTAC 8 cut(s) 139, 516, 696, 885, 891, 1143, 1385, 1413
AfiI CCNNNNNNNGG 9 cut(s) 149, 269, 314, 507, 508, 570, 783, 844, 1122
AgsI TTSAA 5 cut(s) 94, 465, 662, 1183, 1309
AhlI ACTAGT 1 cut(s) 1345
AjnI CCWGG 4 cut(s) 308, 837, 1186, 1326
AleI CACNNNNGTG 1 cut(s) 1066
AluBI AGCT 7 cut(s) 52, 159, 380, 638, 767, 821, 947
AluI AGCT 7 cut(s) 52, 159, 380, 638, 767, 821, 947
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 2 cut(s) 47, 866
AlwI GGATC 1 cut(s) 849
AlwNI CAGNNNCTG 2 cut(s) 176, 844
Ama87I CYCGRG 3 cut(s) 671, 1017, 1084
AoxI GGCC 4 cut(s) 151, 316, 1217, 1323
ApaI GGGCCC 1 cut(s) 320
ApeKI GCWGC 2 cut(s) 290, 1013
ApoI RAATTY 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 1304
AspS9I GGNCC 8 cut(s) 173, 305, 316, 317, 758, 1190, 1324, 1391
AsuHPI GGTGA 6 cut(s) 199, 589, 994, 1053, 1105, 1174
AvaI CYCGRG 3 cut(s) 671, 1017, 1084
AvaII GGWCC 5 cut(s) 173, 305, 758, 1190, 1391
BaeGI GKGCMC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 153
BanI GGYRCC 1 cut(s) 619
BanII GRGCYC 2 cut(s) 320, 382
BbsI GAAGAC 2 cut(s) 640, 1258
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 302, 1000
BccI CCATC 4 cut(s) 189, 1037, 1159, 1290
BciT130I CCWGG 4 cut(s) 310, 839, 1188, 1328
BcoDI GTCTC 2 cut(s) 47, 866
BcuI ACTAGT 1 cut(s) 1345
BfaI CTAG 5 cut(s) 690, 740, 1155, 1346, 1374
BfmI CTRYAG 1 cut(s) 762
BfuAI ACCTGC 2 cut(s) 161, 565
BglI GCCNNNNNGGC 1 cut(s) 299
BisI GCNGC 3 cut(s) 291, 1014, 1217
BlsI GCNGC 3 cut(s) 292, 1015, 1218
BmcAI AGTACT 1 cut(s) 516
Bme1390I CCNGG 4 cut(s) 310, 839, 1188, 1328
Bme18I GGWCC 5 cut(s) 173, 305, 758, 1190, 1391
BmeT110I CYCGRG 3 cut(s) 671, 1017, 1084
BmgT120I GGNCC 8 cut(s) 173, 305, 316, 317, 758, 1190, 1324, 1391
BmiI GGNNCC 7 cut(s) 307, 317, 318, 621, 976, 1098, 1192
BmrFI CCNGG 4 cut(s) 310, 839, 1188, 1328
BmrI ACTGGG 2 cut(s) 441, 690
BmsI GCATC 4 cut(s) 843, 901, 1360, 1441
BmuI ACTGGG 2 cut(s) 441, 690
BpiI GAAGAC 2 cut(s) 640, 1258
BpmI CTGGAG 3 cut(s) 51, 760, 963
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 368
BsaJI CCNNGG 5 cut(s) 237, 308, 1085, 1326, 1327
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 9 cut(s) 149, 269, 314, 507, 508, 570, 783, 844, 1122
Bse1I ACTGG 9 cut(s) 139, 436, 685, 760, 777, 946, 1064, 1220, 1319
Bse3DI GCAATG 1 cut(s) 545
BseBI CCWGG 4 cut(s) 310, 839, 1188, 1328
BseDI CCNNGG 5 cut(s) 237, 308, 1085, 1326, 1327
BseGI GGATG 6 cut(s) 200, 834, 1048, 1052, 1375, 1432
BseLI CCNNNNNNNGG 9 cut(s) 149, 269, 314, 507, 508, 570, 783, 844, 1122
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 3 cut(s) 85, 395, 716
BseNI ACTGG 9 cut(s) 139, 436, 685, 760, 777, 946, 1064, 1220, 1319
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 2 cut(s) 302, 1000
BseYI CCCAGC 1 cut(s) 1122
BsgI GTGCAG 2 cut(s) 765, 882
BshFI GGCC 4 cut(s) 153, 318, 1219, 1325
BshNI GGYRCC 1 cut(s) 619
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 3 cut(s) 671, 1017, 1084
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 9 cut(s) 149, 269, 314, 507, 508, 570, 783, 844, 1122
BsmAI GTCTC 2 cut(s) 47, 866
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 4 cut(s) 153, 318, 1219, 1325
BsoBI CYCGRG 3 cut(s) 671, 1017, 1084
Bsp120I GGGCCC 1 cut(s) 316
Bsp1286I GDGCHC 2 cut(s) 320, 382
Bsp1407I TGTACA 1 cut(s) 1411
Bsp143I GATC 2 cut(s) 841, 1353
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 2 cut(s) 1216, 1436
BspANI GGCC 4 cut(s) 153, 318, 1219, 1325
BspCNI CTCAG 3 cut(s) 84, 394, 717
BspLI GGNNCC 7 cut(s) 307, 317, 318, 621, 976, 1098, 1192
BspMI ACCTGC 2 cut(s) 161, 565
BspPI GGATC 1 cut(s) 849
BspT107I GGYRCC 1 cut(s) 619
BsrDI GCAATG 1 cut(s) 545
BsrGI TGTACA 1 cut(s) 1411
BsrI ACTGG 9 cut(s) 139, 436, 685, 760, 777, 946, 1064, 1220, 1319
BssECI CCNNGG 5 cut(s) 237, 308, 1085, 1326, 1327
BssMI GATC 2 cut(s) 841, 1353
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 4 cut(s) 310, 839, 1188, 1328
Bst4CI ACNGT 3 cut(s) 624, 737, 860
Bst6I CTCTTC 1 cut(s) 1167
BstAPI GCANNNNNTGC 1 cut(s) 176
BstAUI TGTACA 1 cut(s) 1411
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 4 cut(s) 71, 381, 725, 817
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 6 cut(s) 200, 834, 1048, 1052, 1375, 1432
BstKTI GATC 2 cut(s) 844, 1356
BstMAI GTCTC 2 cut(s) 47, 866
BstMBI GATC 2 cut(s) 841, 1353
BstMWI GCNNNNNNNGC 7 cut(s) 176, 299, 377, 392, 401, 1322, 1370
BstNI CCWGG 4 cut(s) 310, 839, 1188, 1328
BstNSI RCATGY 2 cut(s) 124, 487
BstSCI CCNGG 4 cut(s) 308, 837, 1186, 1326
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 302, 1000
BstV2I GAAGAC 2 cut(s) 640, 1258
BstX2I RGATCY 1 cut(s) 841
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 1 cut(s) 841
BsuRI GGCC 4 cut(s) 153, 318, 1219, 1325
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 6 cut(s) 200, 834, 1048, 1052, 1375, 1432
BtsI GCAGTG 1 cut(s) 711
BveI ACCTGC 2 cut(s) 161, 565
Cac8I GCNNGC 1 cut(s) 161
CaiI CAGNNNCTG 2 cut(s) 176, 844
Cfr13I GGNCC 8 cut(s) 173, 305, 316, 317, 758, 1190, 1324, 1391
CseI GACGC 1 cut(s) 29
Csp6I GTAC 8 cut(s) 138, 515, 695, 884, 890, 1142, 1384, 1412
CviAII CATG 7 cut(s) 117, 121, 219, 238, 330, 484, 920
CviQI GTAC 8 cut(s) 138, 515, 695, 884, 890, 1142, 1384, 1412
DdeI CTNAG 4 cut(s) 71, 381, 725, 817
DpnI GATC 2 cut(s) 843, 1355
DpnII GATC 2 cut(s) 841, 1353
EaeI YGGCCR 2 cut(s) 151, 1217
Eam1104I CTCTTC 1 cut(s) 1167
EarI CTCTTC 1 cut(s) 1167
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 2 cut(s) 320, 382
Eco32I GATATC 1 cut(s) 687
Eco47I GGWCC 5 cut(s) 173, 305, 758, 1190, 1391
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 1191
Eco88I CYCGRG 3 cut(s) 671, 1017, 1084
EcoICRI GAGCTC 1 cut(s) 380
EcoO109I RGGNCCY 4 cut(s) 173, 316, 1190, 1391
EcoRII CCWGG 4 cut(s) 308, 837, 1186, 1326
EcoRV GATATC 1 cut(s) 687
EcoT14I CCWWGG 1 cut(s) 237
EcoT22I ATGCAT 2 cut(s) 122, 916
EcoT38I GRGCYC 2 cut(s) 320, 382
ErhI CCWWGG 1 cut(s) 237
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 7 cut(s) 120, 124, 222, 241, 333, 487, 923
FaqI GGGAC 1 cut(s) 448
FatI CATG 7 cut(s) 116, 120, 218, 237, 329, 483, 919
FauNDI CATATG 1 cut(s) 566
FblI GTMKAC 1 cut(s) 677
Fnu4HI GCNGC 3 cut(s) 291, 1014, 1217
FokI GGATG 6 cut(s) 207, 821, 1055, 1059, 1382, 1419
FriOI GRGCYC 2 cut(s) 320, 382
Fsp4HI GCNGC 3 cut(s) 291, 1014, 1217
FspBI CTAG 5 cut(s) 690, 740, 1155, 1346, 1374
GluI GCNGC 3 cut(s) 291, 1014, 1217
GsaI CCCAGC 1 cut(s) 1126
GsuI CTGGAG 3 cut(s) 51, 760, 963
HaeIII GGCC 4 cut(s) 153, 318, 1219, 1325
HgaI GACGC 1 cut(s) 29
Hin1II CATG 7 cut(s) 120, 124, 222, 241, 333, 487, 923
HincII GTYRAC 2 cut(s) 627, 678
HindII GTYRAC 2 cut(s) 627, 678
HindIII AAGCTT 1 cut(s) 157
HinfI GANTC 2 cut(s) 26, 674
HphI GGTGA 6 cut(s) 199, 589, 994, 1053, 1105, 1174
Hpy166II GTNNAC 6 cut(s) 132, 140, 627, 678, 884, 1081
Hpy188I TCNGA 5 cut(s) 74, 230, 363, 411, 726
Hpy188III TCNNGA 6 cut(s) 30, 94, 262, 671, 845, 1243
Hpy8I GTNNAC 6 cut(s) 132, 140, 627, 678, 884, 1081
HpyAV CCTTC 4 cut(s) 69, 220, 918, 988
HpyCH4III ACNGT 3 cut(s) 624, 737, 860
HpyCH4IV ACGT 1 cut(s) 86
HpyF10VI GCNNNNNNNGC 7 cut(s) 176, 299, 377, 392, 401, 1322, 1370
HpyF3I CTNAG 4 cut(s) 71, 381, 725, 817
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 7 cut(s) 120, 124, 222, 241, 333, 487, 923
Kzo9I GATC 2 cut(s) 841, 1353
LmnI GCTCC 2 cut(s) 377, 944
Lsp1109I GCAGC 2 cut(s) 302, 1000
LweI GCATC 4 cut(s) 843, 901, 1360, 1441
MaeI CTAG 5 cut(s) 690, 740, 1155, 1346, 1374
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 5 cut(s) 56, 205, 1000, 1111, 1440
MalI GATC 2 cut(s) 843, 1355
MboI GATC 2 cut(s) 841, 1353
MboII GAAGA 7 cut(s) 205, 246, 633, 640, 973, 1184, 1258
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 2 cut(s) 320, 382
MlsI TGGCCA 1 cut(s) 153
MluCI AATT 6 cut(s) 246, 354, 536, 730, 934, 1309
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 3 cut(s) 208, 1124, 1347
MnlI CCTC 8 cut(s) 153, 443, 512, 621, 1080, 1124, 1250, 1280
Mox20I TGGCCA 1 cut(s) 153
Mph1103I ATGCAT 2 cut(s) 122, 916
MroXI GAANNNNTTC 1 cut(s) 1304
MscI TGGCCA 1 cut(s) 153
MseI TTAA 2 cut(s) 185, 552
MslI CAYNNNNRTG 4 cut(s) 115, 125, 488, 1066
Msp20I TGGCCA 1 cut(s) 153
MspR9I CCNGG 4 cut(s) 310, 839, 1188, 1328
MvaI CCWGG 4 cut(s) 310, 839, 1188, 1328
MwoI GCNNNNNNNGC 7 cut(s) 176, 299, 377, 392, 401, 1322, 1370
NcoI CCATGG 1 cut(s) 237
NdeI CATATG 1 cut(s) 566
NdeII GATC 2 cut(s) 841, 1353
NlaIII CATG 7 cut(s) 120, 124, 222, 241, 333, 487, 923
NlaIV GGNNCC 7 cut(s) 307, 317, 318, 621, 976, 1098, 1192
NmuCI GTSAC 4 cut(s) 205, 1000, 1111, 1440
NsiI ATGCAT 2 cut(s) 122, 916
NspI RCATGY 2 cut(s) 124, 487
OliI CACNNNNGTG 1 cut(s) 1066
PaqCI CACCTGC 1 cut(s) 565
PasI CCCWGGG 1 cut(s) 1327
PdmI GAANNNNTTC 1 cut(s) 1304
PfeI GAWTC 1 cut(s) 26
PflMI CCANNNNNTGG 3 cut(s) 570, 844, 1122
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 3 cut(s) 292, 1015, 1218
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
PpuMI RGGWCCY 3 cut(s) 173, 1190, 1391
PsiI TTATAA 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 382
Psp5II RGGWCCY 3 cut(s) 173, 1190, 1391
Psp6I CCWGG 4 cut(s) 308, 837, 1186, 1326
PspFI CCCAGC 1 cut(s) 1122
PspGI CCWGG 4 cut(s) 308, 837, 1186, 1326
PspN4I GGNNCC 7 cut(s) 307, 317, 318, 621, 976, 1098, 1192
PspOMI GGGCCC 1 cut(s) 316
PspPI GGNCC 8 cut(s) 173, 305, 316, 317, 758, 1190, 1324, 1391
PspPPI RGGWCCY 3 cut(s) 173, 1190, 1391
PstNI CAGNNNCTG 2 cut(s) 176, 844
PsuI RGATCY 1 cut(s) 841
RsaI GTAC 8 cut(s) 139, 516, 696, 885, 891, 1143, 1385, 1413
RsaNI GTAC 8 cut(s) 138, 515, 695, 884, 890, 1142, 1384, 1412
RseI CAYNNNNRTG 4 cut(s) 115, 125, 488, 1066
SacI GAGCTC 1 cut(s) 382
SalI GTCGAC 1 cut(s) 676
SaqAI TTAA 2 cut(s) 185, 552
SatI GCNGC 3 cut(s) 291, 1014, 1217
Sau3AI GATC 2 cut(s) 841, 1353
Sau96I GGNCC 8 cut(s) 173, 305, 316, 317, 758, 1190, 1324, 1391
ScaI AGTACT 1 cut(s) 516
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 4 cut(s) 310, 839, 1188, 1328
SduI GDGCHC 2 cut(s) 320, 382
SfaNI GCATC 4 cut(s) 843, 901, 1360, 1441
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 5 cut(s) 173, 305, 758, 1190, 1391
SmiMI CAYNNNNRTG 4 cut(s) 115, 125, 488, 1066
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SpeI ACTAGT 1 cut(s) 1345
Sse9I AATT 6 cut(s) 246, 354, 536, 730, 934, 1309
SsiI CCGC 2 cut(s) 1216, 1436
SspMI CTAG 5 cut(s) 690, 740, 1155, 1346, 1374
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 4 cut(s) 308, 837, 1186, 1326
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 3 cut(s) 624, 737, 860
TaiI ACGT 1 cut(s) 89
TaqI TCGA 2 cut(s) 677, 1294
TasI AATT 6 cut(s) 246, 354, 536, 730, 934, 1309
TatI WGTACW 3 cut(s) 514, 883, 1411
TauI GCSGC 1 cut(s) 1219
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 2 cut(s) 185, 552
Tru9I TTAA 2 cut(s) 185, 552
TseFI GTSAC 4 cut(s) 205, 1000, 1111, 1440
TseI GCWGC 2 cut(s) 290, 1013
Tsp45I GTSAC 4 cut(s) 205, 1000, 1111, 1440
TspDTI ATGAA 5 cut(s) 105, 318, 406, 529, 908
Van91I CCANNNNNTGG 3 cut(s) 570, 844, 1122
VpaK11BI GGWCC 5 cut(s) 173, 305, 758, 1190, 1391
XapI RAATTY 1 cut(s) 246
XceI RCATGY 2 cut(s) 124, 487
XcmI CCANNNNNNNNNTGG 2 cut(s) 327, 1123
XmiI GTMKAC 1 cut(s) 677
XmnI GAANNNNTTC 1 cut(s) 1304
XspI CTAG 5 cut(s) 690, 740, 1155, 1346, 1374
ZrmI AGTACT 1 cut(s) 516
Zsp2I ATGCAT 2 cut(s) 122, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.