Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001248

Physical Location & Seq

Genomic Coordinates Reverse (-)

35729156 .. 35747570

Gene Structure 18415 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG307300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2262 bp

ATGGATTCAGGGGAAAAGCGACTTAATGAGCTTGGTTACAAGCAAGAACTCAGAAGAGAGATGACTTTGTTCAAAACTCTTGCAATATCATTTTCGACGATGACACTCTTCACCGGAATCACTCCTTTGTATGGTTCTAGCCTTGTATATGCAGGTCCTGCAAGCCTTGTGTGGGGCTGGGTGGTAGTATCATTTTTCACCTGGTTTGTTGGACTTGCCATGGCAGAGATCTGCTCTTCTTTCCCGACCACAGGTTCTCTTTACTTCTGGGCTGCTCACTTAGCTGGCCCCAAATGGGGTCCCCTTGCATCATGGTGTTGTGCTTGGCTTGAGACCATAGGGCTCATTGCTGGCATAGGTACTCAGGCATATGCAGGATCTCAAACACTGCAAAGTATCATTTTACTATGCACTGGGACAAATAAAGGTGGAGGGTATTTTGCACCAAAGTGGTTGTTCTTATGCATGTACATCGGCCTCACTCTTATATGGGCGTTTCTCAACACATTTGCGTTAGAAGTGATTGCCTTTATTGATATAATATCCATATGGTGGCAGGTCGTTGGAGGGACGGTTATTGTTATAATGCTTCCCCTTGTAGCACTTACTACACAGTCTGCATCATACGTGTTCACAAACTTCGATGTGGCCCCTGAGGTGACGGGAATATCAAGCAAGCCTTATGCAGTGATTCTATCCTTTCTTGTTAGTCAGTATTCATTATATGGATATGATGCTGCAGCACATCTAACAGAAGAAACCAGAGGAGCAGACAAGAATGGTCCTATTGCTATTCTAACAAGCATCGGTATCATTTCAGTATTTGGATGGGCATACATCTTGGCCCTGACTTTCAGCATTCAGGATTTTAGCTACCTATATGATCCCACCAATGAGACTAAAGGAGCATTTGTGCCTGCTCAAATTCTCTATGATGCATTTCATGGGAGGTATCATAGTTCCACTGGAGCAATCATTTTACTATTTATCATTTGGGGTTCATTCTTCTTTGGTGGACTTTCAATTACCACCAGTGCTGCCAGAGTAGTGTATGCTCTATCAAGAGACCAAGGGATTCCATTCTCGTCTGTATGGCGAAAAATTCACCCAAAACACAAAGTTCCTTCCAACGCAGTGTGGCTCTGTGCAGCCATCTGCATTCTCCTGGGACTTCCAATCTTGAAAGTTAATGTAGTATTCACGGCCATAACTTCCATATGTACAATTGGATGGGTAGGAGGTTATGCAGTTCCGATCTTTGCAAGAATGGTAATGCCTGAGAAGAAGTTCAACCCCGGGCCATTTTATTTGGGCAATTTAAGCAGACCGATTTGCTTGGTCGCCTTCCTTTGGATTTGTTACACTTGTTCTGTCTTCCTCCTCCCTACTTACTACCCAATTAGCTGGAGTACATTCAATTATGCACCTGTTGCTTTGAGTGTGGGCTTAGGTCTGATAATGCTTTGGTGGGTGTTGGATGCAAGGAAGTGGTTCAAAGGACCTGTTAGAAACATTGAAACTGAACATAATGGAAAGGCACTGATGGCAATGCCTGCTCCCAATTTCAACTTTTGTCTCTCCCTAATTCCAGAAAGAGTGGTTTTCAGAACTGGAAGAATTAAGCGGAAGCAACGAGACTTAATGAAGATTCTCAACAAACATGCTACTAAACCTGTAGATGAAGCTAAACTTGCACATGAAGAATCAAAGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAAGGCAAAAGAAGAACAAGATGGTGGTTCCGTCCTGAACAAAAAAATCTACAGGCTCGGTGATAAGGACCTGCTGGTACTTGTGACAAAGGTTGCTGACAAGATAAAGGTTCATCTGGCAATAGATATCAAAGAGCCAATTAACATTCATTGGGCCCTATCTGTAAACAAAGCTGGAGAGTGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCGAGTTAGGTCTTGCTGGTATTCTGGTGTGGATGAGGTTTATGGCTACGAGGCAGCTGATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAATATTTACACAAGTCATCCACAGTATCGAGAGCTTCTACAGATGATGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATCCAGGTACCGTTTGACTATCCAGCAATTGGTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015179 GO:0015291 GO:0015297 GO:0015318 GO:0015711 GO:0015807 GO:0015849 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1902475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2

Protein Analysis

753

Amino Acids

83.76

Weight (kDa)

9.12

Isoelectric Point (pI)

34.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	22 - 478	4.3e-53	Amino acid permease
AA_permease	PF00324	44 - 497	1.9e-18	Amino acid permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001248)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g24970 FvH4_2g27760 FvH4_2g27770 FvH4_2g27771
malus_domestica	MD00G1162300.v1.1 MD01G1000200.v1.1
prunus_persica	Prupe.1G490100_v2.0.a1 Prupe.1G490200_v2.0.a1 Prupe.1G490200_v2.0.a1 Prupe.1G490300_v2.0.a1 Prupe.4G276400_v2.0.a1 Prupe.4G276400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0122601 RchiOBHm_Chr6g0296631 RchiOBHm_Chr6g0296641 RchiOBHm_Chr6g0296651
rosa_laevigata	RLG00000011673 RLG00000011674 RLG00000011675 RLG00000018694
rosa_multiflora	Rmu_sc0001741.1_g000017 Rmu_sc0001741.1_g000018 Rmu_sc0003133.1_g000008
rosa_roxburghii	Rroxscaffold_7G00171300 Rroxscaffold_7G00171310 Rroxscaffold_7G00171320 Rroxscaffold_7G00178780 Rroxscaffold_7G00178790
rosa_rugosa	Rorug02G0240000 Rorug06G0263800 Rorug06G0263900 Rorug06G0263900
rosa_samantha	Rh2AG298500 Rh2BG307300 Rh2CG285400 Rh2DG322100 Rh6AG375200 Rh6AG375300 Rh6AG375400 Rh6AG375500 Rh6BG383700 Rh6BG383800 Rh6BG383900 Rh6CG388900 Rh6CG389000 Rh6CG389100 Rh6DG376200 Rh6DG376300 Rh6DG376400
rosa_wichuraiana	Rw2G023730 Rw6G032730 Rw6G032740 Rw6G032750 Rw6G032820 Rw6G032830 Rw6G032840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	584
Acc36I	ACCTGC	3 cut(s)	143, 547, 1824
Acc65I	GGTACC	1 cut(s)	2230
AccB1I	GGYRCC	1 cut(s)	2230
AccB7I	CCANNNNNTGG	2 cut(s)	552, 2252
AccI	GTMKAC	1 cut(s)	2162
AciI	CCGC	1 cut(s)	1624
AclWI	GGATC	3 cut(s)	385, 878, 2209
AcoI	YGGCCR	1 cut(s)	1203
AcsI	RAATTY	3 cut(s)	924, 1101, 2202
AcvI	CACGTG	1 cut(s)	2063
AfaI	GTAC	6 cut(s)	361, 470, 1222, 1411, 1824, 2232
AfiI	CCNNNNNNNGG	8 cut(s)	131, 172, 251, 295, 296, 552, 1350, 2252
AflIII	ACRYGT	1 cut(s)	627
AgsI	TTSAA	9 cut(s)	73, 1023, 1183, 1291, 1417, 1495, 1517, 1567, 1726
AjiI	CACGTC	1 cut(s)	2174
AjnI	CCWGG	3 cut(s)	200, 1164, 2226
AleI	CACNNNNGTG	1 cut(s)	448
AluBI	AGCT	9 cut(s)	31, 284, 873, 1404, 1685, 1738, 1919, 2029, 2140
AluI	AGCT	9 cut(s)	31, 284, 873, 1404, 1685, 1738, 1919, 2029, 2140
Alw26I	GTCTC	5 cut(s)	326, 890, 1059, 1580, 1629
AlwI	GGATC	3 cut(s)	385, 878, 2209
AlwNI	CAGNNNCTG	1 cut(s)	158
Ama87I	CYCGRG	1 cut(s)	1295
AoxI	GGCC	7 cut(s)	286, 475, 648, 843, 1203, 1298, 1899
ApaI	GGGCCC	1 cut(s)	1903
ApeKI	GCWGC	7 cut(s)	272, 737, 740, 1037, 1148, 1940, 2026
ApoI	RAATTY	3 cut(s)	924, 1101, 2202
ArsI	GACNNNNNNTTYG	2 cut(s)	2187, 2219
Asp700I	GAANNNNTTC	2 cut(s)	1286, 1490
Asp718I	GGTACC	1 cut(s)	2230
AsuC2I	CCSGG	2 cut(s)	1296, 1297
AsuHPI	GGTGA	5 cut(s)	103, 190, 670, 1097, 1817
AvaI	CYCGRG	1 cut(s)	1295
AvaII	GGWCC	5 cut(s)	155, 299, 782, 1499, 1813
AxyI	CCTNAGG	1 cut(s)	654
BaeGI	GKGCMC	1 cut(s)	1903
BanI	GGYRCC	1 cut(s)	2230
BanII	GRGCYC	2 cut(s)	345, 1903
BbrPI	CACGTG	1 cut(s)	2063
BbsI	GAAGAC	1 cut(s)	1366
BbvI	GCAGC	7 cut(s)	259, 724, 752, 1024, 1160, 1952, 2038
BccI	CCATC	5 cut(s)	822, 1160, 1224, 1537, 1760
BceAI	ACGGC	1 cut(s)	1218
BcgI	CGANNNNNNTGC	2 cut(s)	454, 488
BciT130I	CCWGG	3 cut(s)	202, 1166, 2228
BcnI	CCSGG	2 cut(s)	1296, 1297
BcoDI	GTCTC	5 cut(s)	326, 890, 1059, 1580, 1629
BfaI	CTAG	1 cut(s)	138
BfmI	CTRYAG	4 cut(s)	738, 1674, 1795, 2144
BfuAI	ACCTGC	3 cut(s)	143, 547, 1824
BglII	AGATCT	1 cut(s)	228
BisI	GCNGC	7 cut(s)	273, 738, 741, 1038, 1149, 1941, 2027
BlsI	GCNGC	7 cut(s)	274, 739, 742, 1039, 1150, 1942, 2028
Bme1390I	CCNGG	5 cut(s)	202, 1166, 1296, 1297, 2228
Bme18I	GGWCC	5 cut(s)	155, 299, 782, 1499, 1813
BmeT110I	CYCGRG	1 cut(s)	1295
BmgBI	CACGTC	1 cut(s)	2174
BmiI	GGNNCC	7 cut(s)	289, 300, 301, 651, 1774, 1901, 2232
BmrFI	CCNGG	5 cut(s)	202, 1166, 1296, 1297, 2228
BmrI	ACTGGG	1 cut(s)	423
BmsI	GCATC	7 cut(s)	317, 629, 724, 813, 925, 1468, 1957
BmuI	ACTGGG	1 cut(s)	423
BpiI	GAAGAC	1 cut(s)	1366
BplI	GAGNNNNNCTC	2 cut(s)	218, 250
BpmI	CTGGAG	4 cut(s)	987, 1426, 1941, 2087
Bpu10I	CCTNAGC	1 cut(s)	1447
BpuEI	CTTGAG	1 cut(s)	350
BpuMI	CCSGG	2 cut(s)	1296, 1297
BsaAI	YACGTR	2 cut(s)	628, 2063
BsaI	GGTCTC	2 cut(s)	326, 1059
BsaJI	CCNNGG	6 cut(s)	219, 1069, 1165, 1294, 1295, 1746
BsaWI	WCCGGW	1 cut(s)	113
BsaXI	ACNNNNNCTCC	4 cut(s)	558, 588, 897, 927
Bsc4I	CCNNNNNNNGG	8 cut(s)	131, 172, 251, 295, 296, 552, 1350, 2252
Bse1I	ACTGG	4 cut(s)	418, 970, 1032, 1615
Bse21I	CCTNAGG	1 cut(s)	654
Bse3DI	GCAATG	3 cut(s)	345, 1554, 1935
BseBI	CCWGG	3 cut(s)	202, 1166, 2228
BseDI	CCNNGG	6 cut(s)	219, 1069, 1165, 1294, 1295, 1746
BseGI	GGATG	6 cut(s)	833, 1235, 1483, 2010, 2122, 2194
BseLI	CCNNNNNNNGG	8 cut(s)	131, 172, 251, 295, 296, 552, 1350, 2252
BseMI	GCAATG	3 cut(s)	345, 1554, 1935
BseMII	CTCAG	4 cut(s)	64, 377, 645, 1269
BseNI	ACTGG	4 cut(s)	418, 970, 1032, 1615
BseRI	GAGGAG	3 cut(s)	780, 1370, 2193
BseSI	GKGCMC	1 cut(s)	1903
BseXI	GCAGC	7 cut(s)	259, 724, 752, 1024, 1160, 1952, 2038
BseYI	CCCAGC	1 cut(s)	177
BsgI	GTGCAG	1 cut(s)	1167
BshFI	GGCC	7 cut(s)	288, 477, 650, 845, 1205, 1300, 1901
BshNI	GGYRCC	1 cut(s)	2230
BsiHKCI	CYCGRG	1 cut(s)	1295
BsiSI	CCGG	2 cut(s)	114, 1296
BslFI	GGGAC	5 cut(s)	285, 430, 583, 1182, 2208
BslI	CCNNNNNNNGG	8 cut(s)	131, 172, 251, 295, 296, 552, 1350, 2252
BsmAI	GTCTC	5 cut(s)	326, 890, 1059, 1580, 1629
BsmFI	GGGAC	5 cut(s)	285, 430, 583, 1182, 2208
BsmI	GAATGC	2 cut(s)	858, 1158
BsnI	GGCC	7 cut(s)	288, 477, 650, 845, 1205, 1300, 1901
Bso31I	GGTCTC	2 cut(s)	326, 1059
BsoBI	CYCGRG	1 cut(s)	1295
Bsp120I	GGGCCC	1 cut(s)	1899
Bsp1286I	GDGCHC	2 cut(s)	345, 1903
Bsp1407I	TGTACA	2 cut(s)	468, 1220
Bsp143I	GATC	5 cut(s)	228, 377, 883, 1254, 2214
Bsp19I	CCATGG	1 cut(s)	219
BspACI	CCGC	1 cut(s)	1624
BspANI	GGCC	7 cut(s)	288, 477, 650, 845, 1205, 1300, 1901
BspCNI	CTCAG	4 cut(s)	63, 376, 646, 1270
BspLI	GGNNCC	7 cut(s)	289, 300, 301, 651, 1774, 1901, 2232
BspMAI	CTGCAG	1 cut(s)	742
BspMI	ACCTGC	3 cut(s)	143, 547, 1824
BspPI	GGATC	3 cut(s)	385, 878, 2209
BspQI	GCTCTTC	1 cut(s)	241
BspT107I	GGYRCC	1 cut(s)	2230
BspTNI	GGTCTC	2 cut(s)	326, 1059
BsrDI	GCAATG	3 cut(s)	345, 1554, 1935
BsrGI	TGTACA	2 cut(s)	468, 1220
BsrI	ACTGG	4 cut(s)	418, 970, 1032, 1615
BssECI	CCNNGG	6 cut(s)	219, 1069, 1165, 1294, 1295, 1746
BssMI	GATC	5 cut(s)	228, 377, 883, 1254, 2214
BssT1I	CCWWGG	3 cut(s)	219, 1069, 1746
Bst2UI	CCWGG	3 cut(s)	202, 1166, 2228
Bst4CI	ACNGT	5 cut(s)	574, 615, 2130, 2167, 2235
Bst6I	CTCTTC	3 cut(s)	49, 113, 241
BstAPI	GCANNNNNTGC	4 cut(s)	158, 1430, 1553, 1967
BstAUI	TGTACA	2 cut(s)	468, 1220
BstBAI	YACGTR	2 cut(s)	628, 2063
BstC8I	GCNNGC	8 cut(s)	163, 286, 352, 677, 918, 1554, 1972, 2099
BstDEI	CTNAG	6 cut(s)	50, 280, 363, 654, 1278, 1447
BstDSI	CCRYGG	1 cut(s)	219
BstF5I	GGATG	6 cut(s)	833, 1235, 1483, 2010, 2122, 2194
BstKTI	GATC	5 cut(s)	231, 380, 886, 1257, 2217
BstMAI	GTCTC	5 cut(s)	326, 890, 1059, 1580, 1629
BstMBI	GATC	5 cut(s)	228, 377, 883, 1254, 2214
BstMWI	GCNNNNNNNGC	9 cut(s)	158, 281, 1320, 1430, 1544, 1553, 1691, 1727, 1967
BstNI	CCWGG	3 cut(s)	202, 1166, 2228
BstNSI	RCATGY	2 cut(s)	469, 1664
BstSCI	CCNGG	5 cut(s)	200, 1164, 1294, 1295, 2226
BstSFI	CTRYAG	4 cut(s)	738, 1674, 1795, 2144
BstSLI	GKGCMC	1 cut(s)	1903
BstV1I	GCAGC	7 cut(s)	259, 724, 752, 1024, 1160, 1952, 2038
BstV2I	GAAGAC	1 cut(s)	1366
BstX2I	RGATCY	3 cut(s)	228, 377, 2214
BstXI	CCANNNNNNTGG	1 cut(s)	1404
BstYI	RGATCY	3 cut(s)	228, 377, 2214
Bsu36I	CCTNAGG	1 cut(s)	654
BsuRI	GGCC	7 cut(s)	288, 477, 650, 845, 1205, 1300, 1901
BtgI	CCRYGG	1 cut(s)	219
BtrI	CACGTC	1 cut(s)	2174
BtsCI	GGATG	6 cut(s)	833, 1235, 1483, 2010, 2122, 2194
BtsI	GCAGTG	3 cut(s)	386, 693, 1140
BtsIMutI	CAGTG	7 cut(s)	386, 411, 693, 963, 1039, 1140, 1538
BveI	ACCTGC	3 cut(s)	143, 547, 1824
Cac8I	GCNNGC	8 cut(s)	163, 286, 352, 677, 918, 1554, 1972, 2099
CaiI	CAGNNNCTG	1 cut(s)	158
Cfr9I	CCCGGG	1 cut(s)	1295
CsiI	ACCWGGT	1 cut(s)	200
Csp6I	GTAC	6 cut(s)	360, 469, 1221, 1410, 1823, 2231
CviAII	CATG	6 cut(s)	220, 312, 466, 944, 1661, 1697
CviQI	GTAC	6 cut(s)	360, 469, 1221, 1410, 1823, 2231
DdeI	CTNAG	6 cut(s)	50, 280, 363, 654, 1278, 1447
DpnI	GATC	5 cut(s)	230, 379, 885, 1256, 2216
DpnII	GATC	5 cut(s)	228, 377, 883, 1254, 2214
EaeI	YGGCCR	1 cut(s)	1203
Eam1104I	CTCTTC	3 cut(s)	49, 113, 241
EarI	CTCTTC	3 cut(s)	49, 113, 241
Eco130I	CCWWGG	3 cut(s)	219, 1069, 1746
Eco24I	GRGCYC	2 cut(s)	345, 1903
Eco31I	GGTCTC	2 cut(s)	326, 1059
Eco32I	GATATC	1 cut(s)	1873
Eco47I	GGWCC	5 cut(s)	155, 299, 782, 1499, 1813
Eco72I	CACGTG	1 cut(s)	2063
Eco81I	CCTNAGG	1 cut(s)	654
Eco88I	CYCGRG	1 cut(s)	1295
EcoO109I	RGGNCCY	5 cut(s)	155, 299, 1499, 1813, 1900
EcoRI	GAATTC	1 cut(s)	2202
EcoRII	CCWGG	3 cut(s)	200, 1164, 2226
EcoRV	GATATC	1 cut(s)	1873
EcoT14I	CCWWGG	3 cut(s)	219, 1069, 1746
EcoT22I	ATGCAT	2 cut(s)	467, 940
EcoT38I	GRGCYC	2 cut(s)	345, 1903
ErhI	CCWWGG	3 cut(s)	219, 1069, 1746
FaeI	CATG	6 cut(s)	223, 315, 469, 947, 1664, 1700
FalI	AAGNNNNNCTT	4 cut(s)	664, 696, 1674, 1706
FaqI	GGGAC	5 cut(s)	285, 430, 583, 1182, 2208
FatI	CATG	6 cut(s)	219, 311, 465, 943, 1660, 1696
FauNDI	CATATG	3 cut(s)	370, 548, 1217
FblI	GTMKAC	1 cut(s)	2162
Fnu4HI	GCNGC	7 cut(s)	273, 738, 741, 1038, 1149, 1941, 2027
FokI	GGATG	6 cut(s)	840, 1242, 1490, 2017, 2109, 2201
FriOI	GRGCYC	2 cut(s)	345, 1903
Fsp4HI	GCNGC	7 cut(s)	273, 738, 741, 1038, 1149, 1941, 2027
FspBI	CTAG	1 cut(s)	138
GluI	GCNGC	7 cut(s)	273, 738, 741, 1038, 1149, 1941, 2027
GsaI	CCCAGC	1 cut(s)	181
GsuI	CTGGAG	4 cut(s)	987, 1426, 1941, 2087
HaeIII	GGCC	7 cut(s)	288, 477, 650, 845, 1205, 1300, 1901
HapII	CCGG	2 cut(s)	114, 1296
Hin1II	CATG	6 cut(s)	223, 315, 469, 947, 1664, 1700
HinfI	GANTC	6 cut(s)	5, 117, 691, 1075, 1648, 1703
HpaII	CCGG	2 cut(s)	114, 1296
HphI	GGTGA	5 cut(s)	103, 190, 670, 1097, 1817
Hpy166II	GTNNAC	4 cut(s)	633, 1016, 1912, 2163
Hpy188I	TCNGA	4 cut(s)	53, 1254, 1455, 1607
Hpy188III	TCNNGA	9 cut(s)	244, 863, 1062, 1180, 1589, 1780, 2104, 2135, 2207
Hpy8I	GTNNAC	4 cut(s)	633, 1016, 1912, 2163
Hpy99I	CGWCG	1 cut(s)	100
HpyAV	CCTTC	3 cut(s)	1134, 1354, 2177
HpyCH4III	ACNGT	5 cut(s)	574, 615, 2130, 2167, 2235
HpyCH4IV	ACGT	3 cut(s)	627, 2062, 2173
HpyF10VI	GCNNNNNNNGC	9 cut(s)	158, 281, 1320, 1430, 1544, 1553, 1691, 1727, 1967
HpyF3I	CTNAG	6 cut(s)	50, 280, 363, 654, 1278, 1447
HpySE526I	ACGT	3 cut(s)	627, 2062, 2173
Hsp92II	CATG	6 cut(s)	223, 315, 469, 947, 1664, 1700
KflI	GGGWCCC	1 cut(s)	299
KpnI	GGTACC	1 cut(s)	2234
Kzo9I	GATC	5 cut(s)	228, 377, 883, 1254, 2214
LguI	GCTCTTC	1 cut(s)	241
LmnI	GCTCC	5 cut(s)	767, 905, 968, 1561, 2106
Lsp1109I	GCAGC	7 cut(s)	259, 724, 752, 1024, 1160, 1952, 2038
LweI	GCATC	7 cut(s)	317, 629, 724, 813, 925, 1468, 1957
MabI	ACCWGGT	1 cut(s)	200
MaeI	CTAG	1 cut(s)	138
MaeII	ACGT	3 cut(s)	627, 2062, 2173
MaeIII	GTNAC	4 cut(s)	35, 658, 1358, 1828
MalI	GATC	5 cut(s)	230, 379, 885, 1256, 2216
MboI	GATC	5 cut(s)	228, 377, 883, 1254, 2214
MfeI	CAATTG	2 cut(s)	1224, 2250
MflI	RGATCY	3 cut(s)	228, 377, 2214
MhlI	GDGCHC	2 cut(s)	345, 1903
MmeI	TCCRAC	5 cut(s)	190, 544, 1152, 1455, 2149
Mph1103I	ATGCAT	2 cut(s)	467, 940
MroXI	GAANNNNTTC	2 cut(s)	1286, 1490
MseI	TTAA	7 cut(s)	24, 1188, 1319, 1620, 1640, 1887, 1932
MslI	CAYNNNNRTG	2 cut(s)	313, 448
MspA1I	CMGCKG	1 cut(s)	2029
MspI	CCGG	2 cut(s)	114, 1296
MspR9I	CCNGG	5 cut(s)	202, 1166, 1296, 1297, 2228
MunI	CAATTG	2 cut(s)	1224, 2250
Mva1269I	GAATGC	2 cut(s)	858, 1158
MvaI	CCWGG	3 cut(s)	202, 1166, 2228
MwoI	GCNNNNNNNGC	9 cut(s)	158, 281, 1320, 1430, 1544, 1553, 1691, 1727, 1967
NciI	CCSGG	2 cut(s)	1296, 1297
NcoI	CCATGG	1 cut(s)	219
NdeI	CATATG	3 cut(s)	370, 548, 1217
NdeII	GATC	5 cut(s)	228, 377, 883, 1254, 2214
NlaIII	CATG	6 cut(s)	223, 315, 469, 947, 1664, 1700
NlaIV	GGNNCC	7 cut(s)	289, 300, 301, 651, 1774, 1901, 2232
NmuCI	GTSAC	2 cut(s)	658, 1828
NsiI	ATGCAT	2 cut(s)	467, 940
NspI	RCATGY	2 cut(s)	469, 1664
OliI	CACNNNNGTG	1 cut(s)	448
PciSI	GCTCTTC	1 cut(s)	241
PctI	GAATGC	2 cut(s)	858, 1158
PdmI	GAANNNNTTC	2 cut(s)	1286, 1490
PfeI	GAWTC	6 cut(s)	5, 117, 691, 1075, 1648, 1703
PflMI	CCANNNNNTGG	2 cut(s)	552, 2252
PkrI	GCNGC	7 cut(s)	274, 739, 742, 1039, 1150, 1942, 2028
PmaCI	CACGTG	1 cut(s)	2063
PmlI	CACGTG	1 cut(s)	2063
Ppu21I	YACGTR	2 cut(s)	628, 2063
PpuMI	RGGWCCY	4 cut(s)	155, 299, 1499, 1813
PsiI	TTATAA	1 cut(s)	584
Psp5II	RGGWCCY	4 cut(s)	155, 299, 1499, 1813
Psp6I	CCWGG	3 cut(s)	200, 1164, 2226
PspCI	CACGTG	1 cut(s)	2063
PspFI	CCCAGC	1 cut(s)	177
PspGI	CCWGG	3 cut(s)	200, 1164, 2226
PspN4I	GGNNCC	7 cut(s)	289, 300, 301, 651, 1774, 1901, 2232
PspOMI	GGGCCC	1 cut(s)	1899
PspPPI	RGGWCCY	4 cut(s)	155, 299, 1499, 1813
PstI	CTGCAG	1 cut(s)	742
PstNI	CAGNNNCTG	1 cut(s)	158
PsuI	RGATCY	3 cut(s)	228, 377, 2214
PvuII	CAGCTG	1 cut(s)	2029
RsaI	GTAC	6 cut(s)	361, 470, 1222, 1411, 1824, 2232
RsaNI	GTAC	6 cut(s)	360, 469, 1221, 1410, 1823, 2231
RseI	CAYNNNNRTG	2 cut(s)	313, 448
SapI	GCTCTTC	1 cut(s)	241
SaqAI	TTAA	7 cut(s)	24, 1188, 1319, 1620, 1640, 1887, 1932
SatI	GCNGC	7 cut(s)	273, 738, 741, 1038, 1149, 1941, 2027
Sau3AI	GATC	5 cut(s)	228, 377, 883, 1254, 2214
ScrFI	CCNGG	5 cut(s)	202, 1166, 1296, 1297, 2228
SduI	GDGCHC	2 cut(s)	345, 1903
SexAI	ACCWGGT	1 cut(s)	200
SfaNI	GCATC	7 cut(s)	317, 629, 724, 813, 925, 1468, 1957
SfcI	CTRYAG	4 cut(s)	738, 1674, 1795, 2144
SinI	GGWCC	5 cut(s)	155, 299, 782, 1499, 1813
SmaI	CCCGGG	1 cut(s)	1297
SmiMI	CAYNNNNRTG	2 cut(s)	313, 448
SmlI	CTYRAG	1 cut(s)	329
SmoI	CTYRAG	1 cut(s)	329
SsiI	CCGC	1 cut(s)	1624
SspI	AATATT	1 cut(s)	2110
SspMI	CTAG	1 cut(s)	138
StyD4I	CCNGG	5 cut(s)	200, 1164, 1294, 1295, 2226
StyI	CCWWGG	3 cut(s)	219, 1069, 1746
TaaI	ACNGT	5 cut(s)	574, 615, 2130, 2167, 2235
TaiI	ACGT	3 cut(s)	630, 2065, 2176
TaqI	TCGA	4 cut(s)	95, 642, 2134, 2206
TaqII	GACCGA	1 cut(s)	1342
TatI	WGTACW	3 cut(s)	468, 1220, 1409
TfiI	GAWTC	6 cut(s)	5, 117, 691, 1075, 1648, 1703
Tru1I	TTAA	7 cut(s)	24, 1188, 1319, 1620, 1640, 1887, 1932
Tru9I	TTAA	7 cut(s)	24, 1188, 1319, 1620, 1640, 1887, 1932
TscAI	CASTG	7 cut(s)	393, 418, 693, 970, 1039, 1140, 1545
TseFI	GTSAC	2 cut(s)	658, 1828
TseI	GCWGC	7 cut(s)	272, 737, 740, 1037, 1148, 1940, 2026
Tsp45I	GTSAC	2 cut(s)	658, 1828
TspDTI	ATGAA	8 cut(s)	708, 932, 990, 1658, 1695, 1713, 1847, 1883
TspGWI	ACGGA	1 cut(s)	1765
TspMI	CCCGGG	1 cut(s)	1295
TspRI	CASTG	7 cut(s)	393, 418, 693, 970, 1039, 1140, 1545
Van91I	CCANNNNNTGG	2 cut(s)	552, 2252
VpaK11BI	GGWCC	5 cut(s)	155, 299, 782, 1499, 1813
XapI	RAATTY	3 cut(s)	924, 1101, 2202
XceI	RCATGY	2 cut(s)	469, 1664
XmaI	CCCGGG	1 cut(s)	1295
XmiI	GTMKAC	1 cut(s)	2162
XmnI	GAANNNNTTC	2 cut(s)	1286, 1490
XspI	CTAG	1 cut(s)	138
Zsp2I	ATGCAT	2 cut(s)	467, 940

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.