Rw6G032740

Amino-acid permease BAT1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001248
Physical Location & Seq
Forward (+)
56994168 .. 56996620
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032740.1

Sequence Viewer

Length: 1428 bp
ATGGATCCTGGAGAAAAGCGTCTGAATGAGCTTGGTTATAAGCAAGAACTGAGGAGGGAGATGACGCTGCTCAAGACACTGGCAATAACCTTTTCATGCATATCAGTATTTTCTGGTACACCTCTCTATGGCCAAAGCTTGCGCTATGCAGGTCCTGCGACCTTAATATGGGGTTGGGTAGCTGTCACATTCTTCACATGGTTTGTTGCAATCGCCATGGCTGAGATTTGCTTCTCTTTCCCTACCACTGGTTCTCTTTACTTCTGGGCTTCCCATTTGGCTGGACCCAGGTGGGGCCCGTTTGCTTCGTGGTGCTGTGCTTGGCTTGAGACTACTGGCGTGATATCTGCAATAGGTGCTCAGGCGTATTCTGCATCACAAGCATTGCAGATGATCATCCTTATAGCCACAGGAACAAACAAGGGTCTAATCATCATATGGGCAGTACTAAACACATTTGCCCCACAAGTGATAGCATTTCTCAACATAATTTCCATATGGTGGCAGGTACTTGGTGGTTTGCTTGTGATTATAATGCTTCCTCTGGTGGCACAGACGACACAGTCAGCTTCTTATGTCTTCACACATTTTGAAACTTCTCCGGAGTTGACTGGGATATCTAGCATACCTTATGCAGTGATTTTATCAGTTCTTCTGAGCATCTACTCTCTATACGGTTTTGATGCTGCAGCTCATCTTACTGAGGAGACGAAAGGCGCAGATAGAACTGGTCCTATAGCCATTCTATCTAGTTTTGGGATTATATCAGTGCTTGGTTGGGCTTATTACATAGCTCTCACATTCAGCATCCGGACAATGAGACTGCTGGTGCTTGTACCAGCACAGATAATATACGAAGCATTCTATGGGAGGTTTCATAATTCAAACGGAGCTGTGGCTTTCCTCTGTATCATGTGGGGTTCCTACTTCTTTTGTGGGTTGTCAGTTATCACCACAGCTGCCCGAGTAGTTTATGCTCTATCAAGGGATAATGGCATCCCCTTTTCACCAATTTGGAGGAAAGTGCATCCAAGAAGTAAGGTCCCAACAAATGCAGTGTGGCTCTGCGCAGCCATCGGTATCCTGCTAGGATTACCCATCTTGAAACTTGACTTGGTATTCACAGCCATCATTTCAGTTAGTACAATTGGTTGGGTGGGTGGTTATGCAGTACCTATTTTTGCTAGGCTGGTGATGGTTGAAGAGAAATTCAAAGCAGGACCCTTCTATTTGGGCAGAGGAAGCAGGCCAGTTTGCTTTGTGGCCTTTGTGTGGATATGTTATGCATGTTCTACCTTCTTATTGCCAACTTTCTATCCTCTTAGATGGAAAACTTTCAATTATGCACCAATTGCTCTTAGTGTTGTTTTGACACTGATAATGCTTTGGTGGGTTTTGGATGCAAGGAAAGTTCAAAGGACCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2

Protein Analysis

475

Amino Acids

52.62

Weight (kDa)

9.23

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 24 - 454 4.7e-43 Amino acid permease
AA_permease PF00324 44 - 470 3.4e-19 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 39, 533
AasI GACNNNNNNGTC 1 cut(s) 562
Acc16I TGCGCA 1 cut(s) 1069
Acc36I ACCTGC 2 cut(s) 140, 496
AccB7I CCANNNNNTGG 1 cut(s) 501
AccIII TCCGGA 2 cut(s) 601, 810
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 1 cut(s) 1208
AfaI GTAC 6 cut(s) 118, 447, 510, 837, 1144, 1173
AfiI CCNNNNNNNGG 6 cut(s) 128, 168, 248, 293, 501, 1272
AgsI TTSAA 7 cut(s) 593, 885, 1105, 1202, 1213, 1339, 1415
AjnI CCWGG 2 cut(s) 7, 287
AjuI GAANNNNNNNTTGG 2 cut(s) 1097, 1129
AluBI AGCT 8 cut(s) 31, 138, 182, 569, 692, 794, 893, 959
AluI AGCT 8 cut(s) 31, 138, 182, 569, 692, 794, 893, 959
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 3 cut(s) 323, 701, 814
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 963
Aor13HI TCCGGA 2 cut(s) 601, 810
AoxI GGCC 4 cut(s) 130, 295, 1247, 1263
ApaI GGGCCC 1 cut(s) 299
ApeKI GCWGC 5 cut(s) 67, 686, 689, 959, 1070
ApoI RAATTY 1 cut(s) 1208
Asp700I GAANNNNTTC 1 cut(s) 1334
AspLEI GCGC 3 cut(s) 144, 719, 1070
AspS9I GGNCC 8 cut(s) 152, 284, 295, 296, 731, 1042, 1220, 1419
AsuHPI GGTGA 3 cut(s) 943, 999, 1204
AvaI CYCGRG 1 cut(s) 963
AvaII GGWCC 6 cut(s) 152, 284, 731, 1042, 1220, 1419
BaeGI GKGCMC 1 cut(s) 299
BalI TGGCCA 1 cut(s) 132
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 5 cut(s) 54, 673, 701, 946, 1082
BccI CCATC 5 cut(s) 1082, 1106, 1136, 1189, 1320
BciT130I CCWGG 2 cut(s) 9, 289
BciVI GTATCC 1 cut(s) 1091
BclI TGATCA 1 cut(s) 393
BcoDI GTCTC 3 cut(s) 323, 701, 814
BfaI CTAG 4 cut(s) 621, 750, 1088, 1185
BfmI CTRYAG 2 cut(s) 687, 735
BfuAI ACCTGC 2 cut(s) 140, 496
BfuI GTATCC 1 cut(s) 1091
BisI GCNGC 5 cut(s) 68, 687, 690, 960, 1071
BlsI GCNGC 5 cut(s) 69, 688, 691, 961, 1072
BmcAI AGTACT 1 cut(s) 447
Bme1390I CCNGG 2 cut(s) 9, 289
Bme18I GGWCC 6 cut(s) 152, 284, 731, 1042, 1220, 1419
BmeT110I CYCGRG 1 cut(s) 963
BmgT120I GGNCC 8 cut(s) 152, 284, 295, 296, 731, 1042, 1220, 1419
BmiI GGNNCC 8 cut(s) 6, 286, 296, 297, 922, 1044, 1222, 1421
BmrFI CCNGG 2 cut(s) 9, 289
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 7 cut(s) 383, 669, 673, 816, 1005, 1036, 1390
BmuI ACTGGG 1 cut(s) 621
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 30
Bpu10I CCTNAGC 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 56, 347
BsaBI GATNNNNATC 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 216, 287
BsaWI WCCGGW 2 cut(s) 601, 810
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 168, 248, 293, 501, 1272
Bse1I ACTGG 6 cut(s) 84, 253, 340, 616, 733, 1250
Bse3DI GCAATG 1 cut(s) 383
Bse8I GATNNNNATC 1 cut(s) 395
BseAI TCCGGA 2 cut(s) 601, 810
BseBI CCWGG 2 cut(s) 9, 289
BseDI CCNNGG 2 cut(s) 216, 287
BseGI GGATG 5 cut(s) 396, 807, 996, 1027, 1405
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 6 cut(s) 128, 168, 248, 293, 501, 1272
BseMI GCAATG 1 cut(s) 383
BseMII CTCAG 5 cut(s) 41, 213, 374, 647, 693
BseNI ACTGG 6 cut(s) 84, 253, 340, 616, 733, 1250
BseRI GAGGAG 2 cut(s) 67, 719
BseSI GKGCMC 1 cut(s) 299
BseXI GCAGC 5 cut(s) 54, 673, 701, 946, 1082
BshFI GGCC 4 cut(s) 132, 297, 1249, 1265
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 963
BsiSI CCGG 2 cut(s) 602, 811
BslFI GGGAC 1 cut(s) 1028
BslI CCNNNNNNNGG 6 cut(s) 128, 168, 248, 293, 501, 1272
BsmAI GTCTC 3 cut(s) 323, 701, 814
BsmBI CGTCTC 1 cut(s) 701
BsmFI GGGAC 1 cut(s) 1028
BsmI GAATGC 1 cut(s) 860
BsnI GGCC 4 cut(s) 132, 297, 1249, 1265
BsoBI CYCGRG 1 cut(s) 963
Bsp120I GGGCCC 1 cut(s) 295
Bsp1286I GDGCHC 2 cut(s) 299, 361
Bsp13I TCCGGA 2 cut(s) 601, 810
Bsp143I GATC 2 cut(s) 4, 393
Bsp19I CCATGG 1 cut(s) 216
BspANI GGCC 4 cut(s) 132, 297, 1249, 1265
BspCNI CTCAG 5 cut(s) 42, 214, 373, 648, 694
BspEI TCCGGA 2 cut(s) 601, 810
BspLI GGNNCC 8 cut(s) 6, 286, 296, 297, 922, 1044, 1222, 1421
BspMAI CTGCAG 1 cut(s) 691
BspMI ACCTGC 2 cut(s) 140, 496
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 383
BsrI ACTGG 6 cut(s) 84, 253, 340, 616, 733, 1250
BssECI CCNNGG 2 cut(s) 216, 287
BssMI GATC 2 cut(s) 4, 393
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 9, 289
Bst4CI ACNGT 2 cut(s) 564, 677
Bst6I CTCTTC 1 cut(s) 1197
BstAPI GCANNNNNTGC 3 cut(s) 155, 356, 1352
BstC8I GCNNGC 2 cut(s) 140, 1247
BstDEI CTNAG 7 cut(s) 50, 222, 360, 656, 702, 1322, 1358
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 5 cut(s) 396, 807, 996, 1027, 1405
BstHHI GCGC 3 cut(s) 144, 719, 1070
BstKTI GATC 2 cut(s) 7, 396
BstMAI GTCTC 3 cut(s) 323, 701, 814
BstMBI GATC 2 cut(s) 4, 393
BstMWI GCNNNNNNNGC 6 cut(s) 155, 356, 371, 380, 1242, 1352
BstNI CCWGG 2 cut(s) 9, 289
BstNSI RCATGY 1 cut(s) 1290
BstSCI CCNGG 2 cut(s) 7, 287
BstSFI CTRYAG 2 cut(s) 687, 735
BstSLI GKGCMC 1 cut(s) 299
BstV1I GCAGC 5 cut(s) 54, 673, 701, 946, 1082
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 1091
BsuRI GGCC 4 cut(s) 132, 297, 1249, 1265
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 5 cut(s) 396, 807, 996, 1027, 1405
BtsI GCAGTG 2 cut(s) 642, 1062
BtsIMutI CAGTG 6 cut(s) 77, 246, 642, 774, 1062, 1373
BveI ACCTGC 2 cut(s) 140, 496
Cac8I GCNNGC 2 cut(s) 140, 1247
CaiI CAGNNNCTG 1 cut(s) 155
CfoI GCGC 3 cut(s) 144, 719, 1070
Cfr13I GGNCC 8 cut(s) 152, 284, 295, 296, 731, 1042, 1220, 1419
CseI GACGC 2 cut(s) 8, 73
Csp6I GTAC 6 cut(s) 117, 446, 509, 836, 1143, 1172
CviAII CATG 5 cut(s) 96, 198, 217, 913, 1287
CviQI GTAC 6 cut(s) 117, 446, 509, 836, 1143, 1172
DdeI CTNAG 7 cut(s) 50, 222, 360, 656, 702, 1322, 1358
DpnI GATC 2 cut(s) 6, 395
DpnII GATC 2 cut(s) 4, 393
DrdI GACNNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 1197
EarI CTCTTC 1 cut(s) 1197
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 299
Eco32I GATATC 2 cut(s) 345, 618
Eco47I GGWCC 6 cut(s) 152, 284, 731, 1042, 1220, 1419
Eco88I CYCGRG 1 cut(s) 963
EcoO109I RGGNCCY 5 cut(s) 152, 295, 1042, 1220, 1419
EcoRII CCWGG 2 cut(s) 7, 287
EcoRV GATATC 2 cut(s) 345, 618
EcoT14I CCWWGG 1 cut(s) 216
EcoT22I ATGCAT 2 cut(s) 101, 1288
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 701
FaeI CATG 5 cut(s) 99, 201, 220, 916, 1290
FaqI GGGAC 1 cut(s) 1028
FatI CATG 5 cut(s) 95, 197, 216, 912, 1286
FauNDI CATATG 2 cut(s) 437, 497
FbaI TGATCA 1 cut(s) 393
Fnu4HI GCNGC 5 cut(s) 68, 687, 690, 960, 1071
FokI GGATG 5 cut(s) 383, 794, 983, 1014, 1412
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 5 cut(s) 68, 687, 690, 960, 1071
FspBI CTAG 4 cut(s) 621, 750, 1088, 1185
FspI TGCGCA 1 cut(s) 1069
GlaI GCGC 3 cut(s) 143, 718, 1069
GluI GCNGC 5 cut(s) 68, 687, 690, 960, 1071
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 4 cut(s) 132, 297, 1249, 1265
HapII CCGG 2 cut(s) 602, 811
HgaI GACGC 2 cut(s) 8, 73
HhaI GCGC 3 cut(s) 144, 719, 1070
Hin1II CATG 5 cut(s) 99, 201, 220, 916, 1290
Hin6I GCGC 3 cut(s) 142, 717, 1068
HinP1I GCGC 3 cut(s) 142, 717, 1068
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HindIII AAGCTT 1 cut(s) 136
HpaII CCGG 2 cut(s) 602, 811
HphI GGTGA 3 cut(s) 943, 999, 1204
Hpy166II GTNNAC 2 cut(s) 119, 609
Hpy188I TCNGA 2 cut(s) 24, 657
Hpy188III TCNNGA 4 cut(s) 73, 602, 811, 1102
Hpy8I GTNNAC 2 cut(s) 119, 609
HpyAV CCTTC 2 cut(s) 1234, 1306
HpyCH4III ACNGT 2 cut(s) 564, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 356, 371, 380, 1242, 1352
HpyF3I CTNAG 7 cut(s) 50, 222, 360, 656, 702, 1322, 1358
Hsp92II CATG 5 cut(s) 99, 201, 220, 916, 1290
HspAI GCGC 3 cut(s) 142, 717, 1068
Kpn2I TCCGGA 2 cut(s) 601, 810
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 2 cut(s) 4, 393
LmnI GCTCC 1 cut(s) 890
Lsp1109I GCAGC 5 cut(s) 54, 673, 701, 946, 1082
LweI GCATC 7 cut(s) 383, 669, 673, 816, 1005, 1036, 1390
MaeI CTAG 4 cut(s) 621, 750, 1088, 1185
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 6, 395
MboI GATC 2 cut(s) 4, 393
MboII GAAGA 4 cut(s) 184, 571, 644, 1214
MfeI CAATTG 2 cut(s) 1146, 1350
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 299, 361
MlsI TGGCCA 1 cut(s) 132
MluCI AATT 7 cut(s) 489, 880, 1011, 1146, 1208, 1339, 1350
MluNI TGGCCA 1 cut(s) 132
Mox20I TGGCCA 1 cut(s) 132
Mph1103I ATGCAT 2 cut(s) 101, 1288
MroI TCCGGA 2 cut(s) 601, 810
MroXI GAANNNNTTC 1 cut(s) 1334
MscI TGGCCA 1 cut(s) 132
MseI TTAA 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 132
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 2 cut(s) 602, 811
MspR9I CCNGG 2 cut(s) 9, 289
MunI CAATTG 2 cut(s) 1146, 1350
Mva1269I GAATGC 1 cut(s) 860
MvaI CCWGG 2 cut(s) 9, 289
MwoI GCNNNNNNNGC 6 cut(s) 155, 356, 371, 380, 1242, 1352
NcoI CCATGG 1 cut(s) 216
NdeI CATATG 2 cut(s) 437, 497
NdeII GATC 2 cut(s) 4, 393
NlaIII CATG 5 cut(s) 99, 201, 220, 916, 1290
NlaIV GGNNCC 8 cut(s) 6, 286, 296, 297, 922, 1044, 1222, 1421
NmuCI GTSAC 1 cut(s) 184
NsbI TGCGCA 1 cut(s) 1069
NsiI ATGCAT 2 cut(s) 101, 1288
NspI RCATGY 1 cut(s) 1290
PctI GAATGC 1 cut(s) 860
PdmI GAANNNNTTC 1 cut(s) 1334
PflFI GACNNNGTC 1 cut(s) 562
PflMI CCANNNNNTGG 1 cut(s) 501
PfoI TCCNGGA 1 cut(s) 7
PkrI GCNGC 5 cut(s) 69, 688, 691, 961, 1072
PpuMI RGGWCCY 4 cut(s) 152, 1042, 1220, 1419
PsiI TTATAA 2 cut(s) 39, 533
Psp5II RGGWCCY 4 cut(s) 152, 1042, 1220, 1419
Psp6I CCWGG 2 cut(s) 7, 287
PspGI CCWGG 2 cut(s) 7, 287
PspN4I GGNNCC 8 cut(s) 6, 286, 296, 297, 922, 1044, 1222, 1421
PspOMI GGGCCC 1 cut(s) 295
PspPI GGNCC 8 cut(s) 152, 284, 295, 296, 731, 1042, 1220, 1419
PspPPI RGGWCCY 4 cut(s) 152, 1042, 1220, 1419
PstI CTGCAG 1 cut(s) 691
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 6 cut(s) 118, 447, 510, 837, 1144, 1173
RsaNI GTAC 6 cut(s) 117, 446, 509, 836, 1143, 1172
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 5 cut(s) 68, 687, 690, 960, 1071
Sau3AI GATC 2 cut(s) 4, 393
Sau96I GGNCC 8 cut(s) 152, 284, 295, 296, 731, 1042, 1220, 1419
ScaI AGTACT 1 cut(s) 447
ScrFI CCNGG 2 cut(s) 9, 289
SduI GDGCHC 2 cut(s) 299, 361
SfaNI GCATC 7 cut(s) 383, 669, 673, 816, 1005, 1036, 1390
SfcI CTRYAG 2 cut(s) 687, 735
SinI GGWCC 6 cut(s) 152, 284, 731, 1042, 1220, 1419
SmlI CTYRAG 2 cut(s) 71, 326
SmoI CTYRAG 2 cut(s) 71, 326
Sse9I AATT 7 cut(s) 489, 880, 1011, 1146, 1208, 1339, 1350
SspMI CTAG 4 cut(s) 621, 750, 1088, 1185
StyD4I CCNGG 2 cut(s) 7, 287
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 564, 677
TasI AATT 7 cut(s) 489, 880, 1011, 1146, 1208, 1339, 1350
TatI WGTACW 2 cut(s) 445, 1142
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 6 cut(s) 84, 253, 642, 774, 1062, 1380
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 5 cut(s) 67, 686, 689, 959, 1070
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 84, 866
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 6 cut(s) 84, 253, 642, 774, 1062, 1380
Tth111I GACNNNGTC 1 cut(s) 562
Van91I CCANNNNNTGG 1 cut(s) 501
VpaK11BI GGWCC 6 cut(s) 152, 284, 731, 1042, 1220, 1419
XapI RAATTY 1 cut(s) 1208
XceI RCATGY 1 cut(s) 1290
XmnI GAANNNNTTC 1 cut(s) 1334
XspI CTAG 4 cut(s) 621, 750, 1088, 1185
ZrmI AGTACT 1 cut(s) 447
Zsp2I ATGCAT 2 cut(s) 101, 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.