FvH4_2g27760

Amino-acid permease BAT1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001248
Physical Location & Seq
Reverse (-)
21955966 .. 21959117
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27760.t1

Sequence Viewer

Length: 1578 bp
ATGACTCAACATGTTATGGAAATGGATTCCGGAGAAAAGCGTCTCAATGAACTTGGTTACAAGCAAGAACTCAGAAGGGAAATGACGTTGTTCAAGACACTTGCAATAACATTTTCATGCATGTCAGTGTTCACTGGTACACCTCTCTATGGACAAAGCTTGCATTATGCAGGTCCTGCAACTTTAATATGGGGATGGTTAGTTGTCACCTTCTTCACATGGTTTGTCGGAATCGCCATGGCTGAAATTTGTTCTTCTTTCCCGACTACAGGTTCTCTTTATTTCTGGGCTGCCCATTTGGCCGGACCAAGGTGGGGCCCATTTGCTTCATGGTGCTGTGCTTGGCTTGAGATCATTGGTCTGACTACTGCCATAGGTGCTCAGGCCTATTCAGCAACACAAGCATTTCAGATGTTCATTCTTATAGCCACTGGGACAAACAAGGGTGGAGGCTATTTTGCTTCAAGGAGTGTATTTGTGTGCATGTATATGGGCATAACCCTCATTTGGGCAGTATTAAACACTTTTGCTCTGCAATTCATAGCATTGCTCAACATAATCTCCATATTGTGGCAGGTGATTGGTGGTTTGCTTGTGATTATAATGCTACCTCTGGTGGCACAGTCCACACAAACAGCTTCTTCTGTCTTTACACATTTTGAAACATCTCCTGAGTCGACTGGGATATCCAGTGTACCTTATGCAGTGGTTTTATCAGTGCTTCTGAGCAATTACTGTCTGTATGGGTATGATGCTGCAGCTCATCTTACTGAAGAGACAAAAGGTGCAGATAAAACTGGACCTATAGCTATTCTCTCCAGTATTGGGATAATATCAGTGTTTGGTTGGGCTTATTACTTAGCTCTCACTTTCAGCATCCAGGATCTGGACTATTTATACAGTGCAGACAATGAGACTGCTGGAGTACTTGTACCAGCACAGATAATATATGATGCATTTCATGGAAGGTATCATAATTCCACTGGAGCTGTGATTTTCCTGTTTATTATCTGGGGTTCCTTCTTCTTCTGTGGGCTGTCTGTCACCACTACTGCTGCCCGAGTAGTTTATGCTGTATCAAGGGATAGATGTCTGCCCTTTTCATCAGTGTGGAGAAAAGTAAACCCGAAGAACAAGGTTCCAACAAATGCCGTGTGGCTCTGCGCCGCCATCACTATGCTTCTTGGATTACCCATCTTGAAAATGGACGTAGTATTCATAGCCATCCTGTCAGTTAGCACCATTGGCTGGGTGGGAGGTTATGCTGTACCAATCTTTGCTAGGCTGGTGATGGCTGAAGAGGACTTCAAACCAGGACCCTTCTATTTGGGTAGAGCAAGAAGGCCAGTTTGTTTGGTGGCTTTCCTCTGGATATGTTATACCTGTTCAGTCTTCCTATTGCCAACTTCTTACCCCATCAGATGGAAAACTTTCAACTATGCACCAGTTGCCCTCGGTGTTGTTTTGACACTGGTGATGCTCTGGTGGGCTGTGGATGCAAGGAATTGGTACAAAGGACCTGTAAGAAACATTGATGTACAAAATGGAAACAACCAGAATCCCCAGATACACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2

Protein Analysis

526

Amino Acids

58.17

Weight (kDa)

8.36

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 30 - 487 3.1e-52 Amino acid permease
AA_permease PF00324 51 - 430 2.1e-20 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AarI CACCTGC 1 cut(s) 565
Acc36I ACCTGC 2 cut(s) 161, 565
AccB7I CCANNNNNTGG 4 cut(s) 570, 886, 1248, 1422
AccI GTMKAC 1 cut(s) 677
AccIII TCCGGA 1 cut(s) 29
AciI CCGC 1 cut(s) 1167
AclWI GGATC 1 cut(s) 891
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 2 cut(s) 792, 1317
AfaI GTAC 7 cut(s) 139, 696, 927, 933, 1269, 1511, 1539
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 6 cut(s) 94, 465, 662, 1201, 1309, 1435
AjnI CCWGG 2 cut(s) 879, 1312
AluBI AGCT 6 cut(s) 159, 638, 761, 809, 863, 989
AluI AGCT 6 cut(s) 159, 638, 761, 809, 863, 989
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 3 cut(s) 47, 770, 908
AlwI GGATC 1 cut(s) 891
AlwNI CAGNNNCTG 2 cut(s) 176, 886
Ama87I CYCGRG 1 cut(s) 1059
Aor13HI TCCGGA 1 cut(s) 29
AoxI GGCC 4 cut(s) 300, 316, 384, 1343
ApaI GGGCCC 1 cut(s) 320
ApeKI GCWGC 4 cut(s) 290, 755, 758, 1055
ApoI RAATTY 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 1430
AspLEI GCGC 1 cut(s) 1166
AspS9I GGNCC 7 cut(s) 173, 305, 316, 317, 800, 1316, 1517
AsuHPI GGTGA 5 cut(s) 199, 589, 1036, 1300, 1486
AvaI CYCGRG 1 cut(s) 1059
AvaII GGWCC 5 cut(s) 173, 305, 800, 1316, 1517
BaeGI GKGCMC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 320
BbsI GAAGAC 1 cut(s) 1384
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 4 cut(s) 277, 742, 770, 1042
BccI CCATC 7 cut(s) 189, 1178, 1202, 1232, 1285, 1416, 1424
BceAI ACGGC 1 cut(s) 1136
BciT130I CCWGG 2 cut(s) 881, 1314
BcoDI GTCTC 3 cut(s) 47, 770, 908
BfaI CTAG 1 cut(s) 1281
BfmI CTRYAG 3 cut(s) 267, 756, 804
BfuAI ACCTGC 2 cut(s) 161, 565
BglI GCCNNNNNGGC 1 cut(s) 299
BisI GCNGC 5 cut(s) 291, 756, 759, 1056, 1167
BlsI GCNGC 5 cut(s) 292, 757, 760, 1057, 1168
BmcAI AGTACT 1 cut(s) 927
Bme1390I CCNGG 2 cut(s) 881, 1314
Bme18I GGWCC 5 cut(s) 173, 305, 800, 1316, 1517
BmeT110I CYCGRG 1 cut(s) 1059
BmgT120I GGNCC 7 cut(s) 173, 305, 316, 317, 800, 1316, 1517
BmiI GGNNCC 5 cut(s) 317, 318, 1018, 1140, 1318
BmrFI CCNGG 2 cut(s) 881, 1314
BmrI ACTGGG 2 cut(s) 441, 690
BmsI GCATC 5 cut(s) 742, 885, 943, 1467, 1486
BmuI ACTGGG 2 cut(s) 441, 690
BpiI GAAGAC 1 cut(s) 1384
BpmI CTGGAG 3 cut(s) 802, 942, 1005
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 237, 308, 1453
BsaWI WCCGGW 1 cut(s) 29
BsaXI ACNNNNNCTCC 4 cut(s) 545, 575, 915, 945
Bse3DI GCAATG 1 cut(s) 545
BseAI TCCGGA 1 cut(s) 29
BseBI CCWGG 2 cut(s) 881, 1314
BseDI CCNNGG 3 cut(s) 237, 308, 1453
BseGI GGATG 4 cut(s) 200, 876, 1224, 1501
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 4 cut(s) 85, 395, 663, 716
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 4 cut(s) 277, 742, 770, 1042
BseYI CCCAGC 1 cut(s) 1248
BsgI GTGCAG 2 cut(s) 807, 924
BshFI GGCC 4 cut(s) 302, 318, 386, 1345
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 1059
BsiSI CCGG 2 cut(s) 30, 303
BslFI GGGAC 1 cut(s) 448
BsmAI GTCTC 3 cut(s) 47, 770, 908
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 4 cut(s) 302, 318, 386, 1345
BsoBI CYCGRG 1 cut(s) 1059
Bsp120I GGGCCC 1 cut(s) 316
Bsp1286I GDGCHC 2 cut(s) 320, 382
Bsp13I TCCGGA 1 cut(s) 29
Bsp1407I TGTACA 1 cut(s) 1537
Bsp143I GATC 2 cut(s) 351, 883
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 1 cut(s) 1167
BspANI GGCC 4 cut(s) 302, 318, 386, 1345
BspCNI CTCAG 4 cut(s) 84, 394, 664, 717
BspEI TCCGGA 1 cut(s) 29
BspLI GGNNCC 5 cut(s) 317, 318, 1018, 1140, 1318
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 2 cut(s) 161, 565
BspPI GGATC 1 cut(s) 891
BsrDI GCAATG 1 cut(s) 545
BsrGI TGTACA 1 cut(s) 1537
BssECI CCNNGG 3 cut(s) 237, 308, 1453
BssMI GATC 2 cut(s) 351, 883
BssT1I CCWWGG 2 cut(s) 237, 308
Bst2UI CCWGG 2 cut(s) 881, 1314
Bst4CI ACNGT 3 cut(s) 624, 737, 902
Bst6I CTCTTC 2 cut(s) 768, 1293
BstAPI GCANNNNNTGC 2 cut(s) 176, 1448
BstAUI TGTACA 1 cut(s) 1537
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 5 cut(s) 71, 381, 672, 725, 859
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 4 cut(s) 200, 876, 1224, 1501
BstHHI GCGC 1 cut(s) 1166
BstKTI GATC 2 cut(s) 354, 886
BstMAI GTCTC 3 cut(s) 47, 770, 908
BstMBI GATC 2 cut(s) 351, 883
BstMWI GCNNNNNNNGC 8 cut(s) 176, 299, 377, 392, 401, 1245, 1448, 1496
BstNI CCWGG 2 cut(s) 881, 1314
BstNSI RCATGY 3 cut(s) 14, 124, 487
BstSCI CCNGG 2 cut(s) 879, 1312
BstSFI CTRYAG 3 cut(s) 267, 756, 804
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 4 cut(s) 277, 742, 770, 1042
BstV2I GAAGAC 1 cut(s) 1384
BstX2I RGATCY 1 cut(s) 883
BstYI RGATCY 1 cut(s) 883
BsuRI GGCC 4 cut(s) 302, 318, 386, 1345
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 4 cut(s) 200, 876, 1224, 1501
BtsI GCAGTG 1 cut(s) 711
BveI ACCTGC 2 cut(s) 161, 565
Cac8I GCNNGC 1 cut(s) 161
CaiI CAGNNNCTG 2 cut(s) 176, 886
CfoI GCGC 1 cut(s) 1166
Cfr13I GGNCC 7 cut(s) 173, 305, 316, 317, 800, 1316, 1517
CseI GACGC 1 cut(s) 29
Csp6I GTAC 7 cut(s) 138, 695, 926, 932, 1268, 1510, 1538
CviAII CATG 8 cut(s) 11, 117, 121, 219, 238, 330, 484, 962
CviQI GTAC 7 cut(s) 138, 695, 926, 932, 1268, 1510, 1538
DdeI CTNAG 5 cut(s) 71, 381, 672, 725, 859
DpnI GATC 2 cut(s) 353, 885
DpnII GATC 2 cut(s) 351, 883
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 768, 1293
EarI CTCTTC 2 cut(s) 768, 1293
Eco130I CCWWGG 2 cut(s) 237, 308
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 320
Eco32I GATATC 1 cut(s) 687
Eco47I GGWCC 5 cut(s) 173, 305, 800, 1316, 1517
Eco57I CTGAAG 2 cut(s) 792, 1317
Eco88I CYCGRG 1 cut(s) 1059
EcoO109I RGGNCCY 4 cut(s) 173, 316, 1316, 1517
EcoRII CCWGG 2 cut(s) 879, 1312
EcoRV GATATC 1 cut(s) 687
EcoT14I CCWWGG 2 cut(s) 237, 308
EcoT22I ATGCAT 2 cut(s) 122, 958
EcoT38I GRGCYC 1 cut(s) 320
ErhI CCWWGG 2 cut(s) 237, 308
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 8 cut(s) 14, 120, 124, 222, 241, 333, 487, 965
FaqI GGGAC 1 cut(s) 448
FatI CATG 8 cut(s) 10, 116, 120, 218, 237, 329, 483, 961
FblI GTMKAC 1 cut(s) 677
Fnu4HI GCNGC 5 cut(s) 291, 756, 759, 1056, 1167
FokI GGATG 4 cut(s) 207, 863, 1211, 1508
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 5 cut(s) 291, 756, 759, 1056, 1167
FspBI CTAG 1 cut(s) 1281
GlaI GCGC 1 cut(s) 1165
GluI GCNGC 5 cut(s) 291, 756, 759, 1056, 1167
GsaI CCCAGC 1 cut(s) 1252
GsuI CTGGAG 3 cut(s) 802, 942, 1005
HaeIII GGCC 4 cut(s) 302, 318, 386, 1345
HapII CCGG 2 cut(s) 30, 303
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 1166
Hin1II CATG 8 cut(s) 14, 120, 124, 222, 241, 333, 487, 965
Hin6I GCGC 1 cut(s) 1164
HinP1I GCGC 1 cut(s) 1164
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HindIII AAGCTT 1 cut(s) 157
HinfI GANTC 5 cut(s) 4, 26, 231, 674, 1558
HpaII CCGG 2 cut(s) 30, 303
HphI GGTGA 5 cut(s) 199, 589, 1036, 1300, 1486
Hpy166II GTNNAC 6 cut(s) 132, 140, 627, 678, 695, 1123
Hpy188I TCNGA 6 cut(s) 74, 230, 363, 411, 726, 1421
Hpy188III TCNNGA 7 cut(s) 30, 94, 262, 671, 887, 1198, 1369
Hpy8I GTNNAC 6 cut(s) 132, 140, 627, 678, 695, 1123
HpyAV CCTTC 6 cut(s) 69, 220, 960, 1030, 1330, 1335
HpyCH4III ACNGT 3 cut(s) 624, 737, 902
HpyCH4IV ACGT 2 cut(s) 86, 1209
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 299, 377, 392, 401, 1245, 1448, 1496
HpyF3I CTNAG 5 cut(s) 71, 381, 672, 725, 859
HpySE526I ACGT 2 cut(s) 86, 1209
Hsp92II CATG 8 cut(s) 14, 120, 124, 222, 241, 333, 487, 965
HspAI GCGC 1 cut(s) 1164
Kpn2I TCCGGA 1 cut(s) 29
Kzo9I GATC 2 cut(s) 351, 883
LmnI GCTCC 1 cut(s) 986
Lsp1109I GCAGC 4 cut(s) 277, 742, 770, 1042
LweI GCATC 5 cut(s) 742, 885, 943, 1467, 1486
MaeI CTAG 1 cut(s) 1281
MaeII ACGT 2 cut(s) 86, 1209
MaeIII GTNAC 3 cut(s) 56, 205, 1042
MalI GATC 2 cut(s) 353, 885
MboI GATC 2 cut(s) 351, 883
MboII GAAGA 9 cut(s) 205, 246, 633, 785, 1015, 1018, 1141, 1310, 1384
MflI RGATCY 1 cut(s) 883
MhlI GDGCHC 2 cut(s) 320, 382
MluCI AATT 5 cut(s) 246, 536, 730, 976, 1504
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 2 cut(s) 208, 1166
MnlI CCTC 8 cut(s) 153, 443, 512, 621, 1250, 1294, 1376, 1463
Mph1103I ATGCAT 2 cut(s) 122, 958
MroI TCCGGA 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 1430
MseI TTAA 2 cut(s) 185, 518
MslI CAYNNNNRTG 5 cut(s) 115, 125, 488, 1108, 1175
MspI CCGG 2 cut(s) 30, 303
MspR9I CCNGG 2 cut(s) 881, 1314
MvaI CCWGG 2 cut(s) 881, 1314
MwoI GCNNNNNNNGC 8 cut(s) 176, 299, 377, 392, 401, 1245, 1448, 1496
NcoI CCATGG 1 cut(s) 237
NdeII GATC 2 cut(s) 351, 883
NlaIII CATG 8 cut(s) 14, 120, 124, 222, 241, 333, 487, 965
NlaIV GGNNCC 5 cut(s) 317, 318, 1018, 1140, 1318
NmuCI GTSAC 2 cut(s) 205, 1042
NsiI ATGCAT 2 cut(s) 122, 958
NspI RCATGY 3 cut(s) 14, 124, 487
PaqCI CACCTGC 1 cut(s) 565
PceI AGGCCT 1 cut(s) 386
PciI ACATGT 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 1430
PfeI GAWTC 3 cut(s) 26, 231, 1558
PflMI CCANNNNNTGG 4 cut(s) 570, 886, 1248, 1422
PfoI TCCNGGA 1 cut(s) 879
PkrI GCNGC 5 cut(s) 292, 757, 760, 1057, 1168
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
PpuMI RGGWCCY 3 cut(s) 173, 1316, 1517
PscI ACATGT 1 cut(s) 10
PsiI TTATAA 1 cut(s) 602
Psp5II RGGWCCY 3 cut(s) 173, 1316, 1517
Psp6I CCWGG 2 cut(s) 879, 1312
PspFI CCCAGC 1 cut(s) 1248
PspGI CCWGG 2 cut(s) 879, 1312
PspN4I GGNNCC 5 cut(s) 317, 318, 1018, 1140, 1318
PspOMI GGGCCC 1 cut(s) 316
PspPI GGNCC 7 cut(s) 173, 305, 316, 317, 800, 1316, 1517
PspPPI RGGWCCY 3 cut(s) 173, 1316, 1517
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 2 cut(s) 176, 886
PsuI RGATCY 1 cut(s) 883
RsaI GTAC 7 cut(s) 139, 696, 927, 933, 1269, 1511, 1539
RsaNI GTAC 7 cut(s) 138, 695, 926, 932, 1268, 1510, 1538
RseI CAYNNNNRTG 5 cut(s) 115, 125, 488, 1108, 1175
SalI GTCGAC 1 cut(s) 676
SaqAI TTAA 2 cut(s) 185, 518
SatI GCNGC 5 cut(s) 291, 756, 759, 1056, 1167
Sau3AI GATC 2 cut(s) 351, 883
Sau96I GGNCC 7 cut(s) 173, 305, 316, 317, 800, 1316, 1517
ScaI AGTACT 1 cut(s) 927
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 2 cut(s) 881, 1314
SduI GDGCHC 2 cut(s) 320, 382
SfaNI GCATC 5 cut(s) 742, 885, 943, 1467, 1486
SfcI CTRYAG 3 cut(s) 267, 756, 804
SinI GGWCC 5 cut(s) 173, 305, 800, 1316, 1517
SmiMI CAYNNNNRTG 5 cut(s) 115, 125, 488, 1108, 1175
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 5 cut(s) 246, 536, 730, 976, 1504
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 1 cut(s) 1167
SspMI CTAG 1 cut(s) 1281
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 2 cut(s) 879, 1312
StyI CCWWGG 2 cut(s) 237, 308
TaaI ACNGT 3 cut(s) 624, 737, 902
TaiI ACGT 2 cut(s) 89, 1212
TaqI TCGA 1 cut(s) 677
TasI AATT 5 cut(s) 246, 536, 730, 976, 1504
TatI WGTACW 2 cut(s) 925, 1537
TauI GCSGC 1 cut(s) 1169
TfiI GAWTC 3 cut(s) 26, 231, 1558
Tru1I TTAA 2 cut(s) 185, 518
Tru9I TTAA 2 cut(s) 185, 518
TseFI GTSAC 2 cut(s) 205, 1042
TseI GCWGC 4 cut(s) 290, 755, 758, 1055
Tsp45I GTSAC 2 cut(s) 205, 1042
TspDTI ATGAA 8 cut(s) 63, 105, 318, 406, 529, 950, 1092, 1207
Van91I CCANNNNNTGG 4 cut(s) 570, 886, 1248, 1422
VpaK11BI GGWCC 5 cut(s) 173, 305, 800, 1316, 1517
XapI RAATTY 1 cut(s) 246
XceI RCATGY 3 cut(s) 14, 124, 487
XcmI CCANNNNNNNNNTGG 3 cut(s) 327, 1201, 1249
XmiI GTMKAC 1 cut(s) 677
XmnI GAANNNNTTC 1 cut(s) 1430
XspI CTAG 1 cut(s) 1281
ZrmI AGTACT 1 cut(s) 927
Zsp2I ATGCAT 2 cut(s) 122, 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.