FvH4_2g22100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002148
Physical Location & Seq
Forward (+)
18217629 .. 18218141
513 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22100.t1

Sequence Viewer

Length: 291 bp
ATGGGTTTTCGGAATGTTGGCATCGCAGTTGCAGCACTGCTCTTGCTATTGTTCATGACTCTGCACCTTTCTGAGGCCGGCAGGGTGTTTCTTGCTGGGGAAGAAATCTCTACCATGAACGAACTTTTCGTTTTAATGGCGTCGATCGGAACAGAGGATAGGGGTGCTCCTCCTCCTGATGGACATAATCCGCGCAGCAACGTCCCTGGTCCTACGAGTCCTCAGAGTCATGCCGACTCAACGTTTGCAGCAAGCCACATTACTATGCCGCCTTCAAGTCTCCGGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.19

Weight (kDa)

5.67

Isoelectric Point (pI)

60.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 193
AciI CCGC 2 cut(s) 191, 269
AclI AACGTT 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 2 cut(s) 73, 179
AgsI TTSAA 1 cut(s) 276
AjnI CCWGG 1 cut(s) 205
Alw21I GWGCWC 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 284
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 32, 195, 248
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 209
AvaII GGWCC 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 3 cut(s) 44, 207, 260
BccI CCATC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 284
BisI GCNGC 4 cut(s) 33, 196, 249, 269
BlsI GCNGC 4 cut(s) 34, 197, 250, 270
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 282
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 179
Bse118I RCCGGY 1 cut(s) 77
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 205
BseLI CCNNNNNNNGG 2 cut(s) 73, 179
BseMII CTCAG 2 cut(s) 63, 236
BseRI GAGGAG 2 cut(s) 159, 162
BseXI GCAGC 3 cut(s) 44, 207, 260
BseYI CCCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 169
BsiSI CCGG 2 cut(s) 78, 283
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 2 cut(s) 73, 179
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 144
BspACI CCGC 2 cut(s) 191, 269
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 2 cut(s) 64, 235
BspFNI CGCG 1 cut(s) 193
BspHI TCATGA 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 144
BssNI GRCGYC 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 79, 253
BstDEI CTNAG 2 cut(s) 72, 222
BstENI CCTNNNNNAGG 1 cut(s) 71
BstFNI CGCG 1 cut(s) 193
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 1 cut(s) 144
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 205
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 3 cut(s) 44, 207, 260
BsuRI GGCC 1 cut(s) 77
BtgZI GCGATG 1 cut(s) 7
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 79, 253
CciI TCATGA 1 cut(s) 54
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 1 cut(s) 129
Csp6I GTAC 1 cut(s) 286
CviAII CATG 3 cut(s) 55, 115, 230
CviJI RGCY 2 cut(s) 77, 255
CviKI_1 RGCY 2 cut(s) 77, 255
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 2 cut(s) 72, 222
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 209
EcoNI CCTNNNNNAGG 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 205
FaeI CATG 3 cut(s) 58, 118, 233
FaiI YATR 5 cut(s) 56, 116, 186, 231, 266
FaqI GGGAC 1 cut(s) 188
FatI CATG 3 cut(s) 54, 114, 229
Fnu4HI GCNGC 4 cut(s) 33, 196, 249, 269
Fsp4HI GCNGC 4 cut(s) 33, 196, 249, 269
GlaI GCGC 1 cut(s) 194
GluI GCNGC 4 cut(s) 33, 196, 249, 269
GsaI CCCAGC 1 cut(s) 99
HaeIII GGCC 1 cut(s) 77
HapII CCGG 2 cut(s) 78, 283
HgaI GACGC 1 cut(s) 129
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 3 cut(s) 58, 118, 233
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 4 cut(s) 58, 217, 226, 236
HpaII CCGG 2 cut(s) 78, 283
Hpy188I TCNGA 4 cut(s) 12, 73, 149, 225
Hpy188III TCNNGA 2 cut(s) 55, 176
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 282
HpyCH4IV ACGT 2 cut(s) 201, 242
HpyCH4V TGCA 3 cut(s) 32, 64, 248
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 2 cut(s) 72, 222
HpySE526I ACGT 2 cut(s) 201, 242
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 3 cut(s) 58, 118, 233
HspAI GCGC 1 cut(s) 193
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 172
LpnPI CCDG 6 cut(s) 67, 81, 91, 189, 192, 219
Lsp1109I GCAGC 3 cut(s) 44, 207, 260
LweI GCATC 1 cut(s) 30
MaeII ACGT 2 cut(s) 201, 242
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MboII GAAGA 1 cut(s) 113
MhlI GDGCHC 1 cut(s) 169
MlyI GAGTC 4 cut(s) 52, 226, 230, 235
MnlI CCTC 5 cut(s) 67, 148, 180, 183, 231
MroNI GCCGGC 1 cut(s) 77
MseI TTAA 1 cut(s) 134
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 2 cut(s) 78, 283
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 32
NaeI GCCGGC 1 cut(s) 79
NdeII GATC 1 cut(s) 144
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 3 cut(s) 58, 118, 233
PagI TCATGA 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdiI GCCGGC 1 cut(s) 79
PkrI GCNGC 4 cut(s) 34, 197, 250, 270
Ple19I CGATCG 1 cut(s) 147
PleI GAGTC 4 cut(s) 52, 225, 230, 234
PpsI GAGTC 4 cut(s) 52, 225, 230, 234
Psp1406I AACGTT 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 1 cut(s) 205
PspPI GGNCC 1 cut(s) 209
PvuI CGATCG 1 cut(s) 147
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 1 cut(s) 134
SatI GCNGC 4 cut(s) 33, 196, 249, 269
Sau3AI GATC 1 cut(s) 144
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 4 cut(s) 52, 226, 230, 235
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 169
SetI ASST 3 cut(s) 69, 204, 245
SfaNI GCATC 1 cut(s) 30
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 263
SsiI CCGC 2 cut(s) 191, 269
StyD4I CCNGG 1 cut(s) 205
TaiI ACGT 2 cut(s) 204, 245
TaqI TCGA 1 cut(s) 143
TauI GCSGC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TscAI CASTG 1 cut(s) 42
TseI GCWGC 3 cut(s) 32, 195, 248
TspDTI ATGAA 2 cut(s) 43, 131
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 209
XagI CCTNNNNNAGG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.