Rorug06G0039200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002148
Physical Location & Seq
Forward (+)
4871647 .. 4897470
25824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0039200.1

Sequence Viewer

Length: 7398 bp
ATGGCCGTCTCGGTGCAGTCCCTCAGGTTCGGTGGCCCCGTCTCCGCCCCACCCGGCGGCGGAAGCTTCGACGCCCTCAATCGTATCCTCGCCGACCTCTGCACGCGTGGCAACCCCAAGGAGGGGGCTTCGCTGGCTTTGAAGAAGCATCTGGAAGAAGAAGCCCGTGACTTAAGTGGAGAAGCATTTTCTCGTTTCATGGATCAGTTGTATGATCGGATTTCTGCTCTTTTGGATAGCAGTGATGTTGCAGAAAATTTGGGAGCTCTAAGAGCTATTGACGAGTTGATAGATGTGGCACTTGGAGAGAATGCATCAAAGGTTTCAAAGTTTGCTAATTACATAAGGACTGCCTTTGAGCTAAAACGTGATCCTGACATTTTGGTCCTGGCTAGTAGAGTTTTGGGCCATCTAGCTAGGGCCGGGGGAGCAATGACTGCAGATGAAGTGGAATGCCAGATAAAAATTGCTCTGGAATGGTTGCGTGGAGACAGAATTGAGTATCGACGTTTTGCTGCAGTCTTGATTTTGAAAGAAATGGCAGAAAATGCTTCCACAGTGTTCAATGTTCATGTACCTGAGTTTGTTGATGCTATCTGGGTTGCTTTGAGAGATCCAGTATTGCCTATCCGAGAGCGAGCTGTGGAAGCTCTGCGTGCTTGCCTTGGTGTTATTGAGAAACGTGAGACACGATGGCGTGTACAATGGTATTATCGTATGTTCGAAGCTACACAGGATGGATTGGGTAAAAATGCTTCTGTTCACAGCATACATGGTTCTTTACTTGCAGTTGGAGAGCTTTTAAGGAATACAGGTGAATTCATGATGTCAAGGTATAGGGAAGTTGCAGAAATTGTCCTTAGGTATCTTGACCACAAGGATAGGCTGGTTCGCCTGAGCATAACTTCACTGCTACCTCGAATTGCTCATTTTCTGCGTGATCGATTTGTTACAAACTACTTAGAGACATGTATGAATCATATCCTCGCTGTTCTACGACAATCAGCTGAACTACGCTCCAGTGGTTTTATTGCACTTGGGGAGATGGCCGGTGCTTTGGACGGGGAACTGTTTTTGTATTTGGGCCAAATTACACCTCACTTACGTGAAGCAATTGCTCCACGTAGAGGGCGACCCTCACTTGAGGCTTTGGCTTGTGTTGGAAATATTGCAAAAGCAATGGGGCCTGCTATGGAACATGATGTTCGTGGTCTTTTGGATGTTATGTTCGCTGCTGGTCTTTCCCCTACGCTTGTTGAGGCCCTTGAAAAGATAACTACCAGTATACCATCTTTGCTGCCTACCATCCAAGATCGGTTGCTAGAATGCATTTCAGTGGTTCTTTCAAAATCCCAACATCCTCAGGGAAGGCCAGTTGTTGGAATGGGTAGAGGAAATTTAATAAATATCCCTCAGCAAGTTTCAGACCTCAGTGGCTCATCCCTTGTGCAGCTTGCTTTGCAGACTCTAGCTCGTTTCAATTTCAAGGGTCACGACCTTCTTGAATTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGACGGAGCCATCCGAAAAGATGCTGCCCTATGTTGTTGCAGATTGGTTGCAAATTCCTTTTCTGGTGTACAATATGCTTCTGGTAGGTCAAATCGGGGAAAGCGACGCCGTCTTGTCGAGGAGATTGTTGAAAAGCTTCTCATTGAGGCTGTTGCAGATGCTGATGTGATTGTTCGCCATTCAATATTTTCTTCTCTACATGGGAATAGAGGCTTTGACGATTTCCTGGCTCAGGCTGACAGTCTCAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGATGTTCGCGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAATCCTGCTTATGTTCTTCCAGCTCTTCGCCGCCATCTTATACAACTGTTAACTTACTTGGGTCAGAGCAGTGCAGATAGCAAATGCAGGGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGTAACTGTGAAAGACTAATACTTCCATACATTGCTCCTATACACAAGGCACTTGTGGCAAGACTCTTGGATGGCACTGGTGTCGGCTCGAATAATGGCATCATTAGTGGAGTTCTTGTAACTGTTGGGGATCTTGCTAGAGTGGGCGGGTTTGCAATGAGAAAATATATACCAGAGCTTATGCCTTTGATCGTTGATGCTTTATTGGATGGAGCGGCTGTCACAAAACGGGAAGTGGCTGTAGCTACTCTCGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCGTATAATGAATATCCACTGTTACTTGGCTTACTCTTGAAGTTGCTTAACGGTGAGTTGGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTTGGAATTATGGGTGCTCTAGATCCTCATGTGCATAAACGAAATCAGCAAAGCTTGCCTGGGTCACATGGAGATGTTCCTCGTAATGCCAGTGATTCTGGTCAACACATCCAATCTGTGGATGAATTGCCTATGGACTTGTGGCCATCATTTGCAACCTCTGAAGATTATTATTCTACGGTTGCAATCAATTCTCTCATGCGAATACTTAGGGACCCTTCACTTGGTACTTACCACCTAAAGGTTGTCGGATCACTTATGTTCATATTCAAGTCAATGGGTCTCGGTTGTGTTCCGTACTTACCAAAGGTTTTGCCTGACCTTTTCCATATAGTCCGCACATGTGATGATGCTTTGAAGGATTTTATAACATGGAAGCTTGGAACTCTAGTGTCTATTGTGCGTCAGCACATACGGAAATATCTGCATGAATTGTTGGTTCTAATTTCAGAACTATGGTCAACCTTTAGTTTCCCTGCTGGTGTCCGTCCTCAACCTGGCTATCCAGTTCTTCATCTGGTGGAGCAGCTTTGTTTGGCCCTGAATGACGAGTTCAGGACGTATCTTCCTGATATTCTTCCATGCTGTATCCAAGTTCTAAGTGACGCAGAACGGTGTAATGACTACACTTACGTTCTTGACATCCTCCATACACTTGAAGTATTTAGTGGAACATTGGATGAACATATGCATCTTCTTCTTCCCGCTCTAATCCGTTTGTTCAAAGTGGATGCTTCAGTGGATATAAGACGTGCTGCTATCAAAACTTTGACCAAACTCATCCCTCGGGTGCAGGTTACTGGTCACATATCTTCTCTTGTGCATCACTTGAAGCTAGTCTTAGATGGGAAGAATGATGACCTCCGGAAGGACACTGTCGATGCGCTTTGTTGTCTAGCTCATGCCCTTGGGGAGGATTTCACCATCTTTATCCCATCCATACACAAACTTTTGTTAAAACATCGCTTACGGCACAAGGGGTTTGAGGAAATTGAAGGCCATTTGCAGCGACGTGAACCTTTGGCTCCCCAAAGATTAAGTCGACGACTTCCAGAGGTTGTTGCTGACCGTTCAACTGATTTGGAGATTGACCCTTATGATGATGTGACTGATGTTCAGAAAAAACTTCGAAGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACACTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCACTACGAACCTGTGCAAGGCTTGCACAGTTGCAGCCATTTGTTGGGCGGGAGTTGTTTGCAGCTGGATTTGTTAGCTGTTGGGCACAGCTAAACGAGACTAGTCAGAAACAGTTAGTTCGGAGTTTGGAGATGGCATTTTCATCTCCAAATATTCCTCCTGAAATTTTGGCAACACTGCTTAATCTGGCAGAGTTCATGGAACATGATGAGAAACCCCTTCCTATAGACATTCGCCTACTTGGTGCTCTTGCAGAGAAGTGTCGTGCATTTGCAAAGGCCTTGCATTACAAAGAAATGGAATTTGAAGGAGCACGTTCCAAGAAGATGGATGCCAATCCTGTTGCTGTAGTTGAAGCACTTATACATATAAACAACCAGTTACAACAGCATGAGGCTGCAGTTGGAATATTGACCTATGCCCAGCAAAATTTGGATGTTCAACTGAAAGAGTCATGGTATGAGAAGTTGCAGCGATGGGATGATGCTCTCAAGGCCTACACTGCAAAAGCATCTCAAGCATCGAGTCAACATCTTATTTTGGATGCTACTTTAGGTCGTATGCGATGCCTTGCTGCCTTGGCTCGATGGGAAGAGCTTAACAACCTGTTTAAGGAACACTGGACCCCAGCTGAGCCTGCTGCTCGACTGGAAATGGCCCCATTGGCTGCAAGTGCTGCTTGGAATATGGGAGAGTGGGATCAAATGGCCGAATATGTGTCTCGATTGGACGATGGTGATGAAACCAAACTTAGGGGATTGGGGAACACTGCTGCTAGTGGAGATGGAAGCAGTAATGGCACATTCTTTAGAGCTGTTTTGTTAGTTCGAAGAGGCAAGTATGATGAAGCACGCGAGTATGTTGAGAGAGCCAGGAAATGCTTGGCAACAGAGCTTGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATAGCAATATGGTTCGTGTTCAGCAGCTGTCAGAACTTGAGGAGGTCATCGATTATTGTACACTTCCTCTGGGGAATCCTGTTGCTGAAGGACGACGGGCCCTTATTCGCAGTATGTGGAATGAGCGGATACAAGGAGCAAAACGTAATGTTGAGGTATGGCAGGTGCTTTTAGCAGTGAGAGCACTTGTTCTACCTCCTACTGAAGATGTTGATACTTGGCTAAAGTTTGCTACCCTCTGTCGGAAAAGTGGGCGACTTAGCCAGGCTAGATCTACTTTAGTCAAACTCTTACAGTATGACCCCGAGACATCTCATGAAAGCGTGCAATACCATGGGCCTCCACAAGTGATGCTTGCATACTTGAAATACCAATGGTCACTTGGAGAAGACCTCAAACGCAAGGAAGCGTTTGCTAGGCTGCAGAATTTGGCAATGGAGCTCTCAACTTCACCTACTATTGAATCAGTTACGCCAACTGGCTTGATGAGCTGTAGTACTCCAAGTGTTCCACTCATTGCCCGTGTGTATCTCAAACTCGGAGAATGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCGTGTTGCCTTCCGAAATGCAACTCAATGTGCAAATAAATGGGCAAAGGCTTGGCACACATGGGCATTGTTCAATACAGCTGTTATGTCTCTTTATACTGTCAGAGGGTATGCAAGTGCTGCTTCCCAGTTTGTTGTTGCAGCAGTCACTGGATATTTTCACTCAATCGCCTGTTCAGCAAATACTAAAGGTGTTGATGATAGCTTACAGGATATTCTTCGTCTTCTTACATTGTGGTTCAACCATGGAGCTAGTGCTGAAGTTCAAATGGCGTTACAAAAAGGGTTTGCACATGTTAATATTAATACATGGCTGGTGGTTTTACCCCAAATAATTGCCAGGATACATTCTAATACTCATGCAGTAAGGGAACTCATACAGTCGCTTTTGGTGCGAATTGGACAAAGTCATCCACAGGCTCTTATGTACCCTCTCCTAGTAGCCTGTAAATCAATAAGTAATTTACGCAAAGCTGCCGCTCAAGAAGTGGTTGAAAAAGTTCGACAGCATAGTGGTTTACTTGTGGATCAGGCACAACTTGTTTCAACGGAACTAATCCGGGTCGCAATACTTTGGCATGAAATGTGGCATGAGGCTCTGGAAGAAGCTAGTCGTCTGTATTTTGGTGAACATAACATTGAGGGCATGCTAAAAGTATTGGAGCCATTGCATGAAATGCTCGAGGAAGGGGCTATGAAGAATAATACCACAATAAAAGAGACAACATTCATTGAGGCCTATCGTCATGAATTGCTGGAGGCTTATGAATGTTGCATGAAATATAAGAGAACTGGAAAAGATGCCGAGCTTACTCAGGCATGGGATCTTTACTATCATGTATTCAGACGAATAGATAAGCAGCTGCAAAGTCTCACTACATTGGACTTGGAGTCTGTCTCGCCAGAGTTGTTAGAATGTCGTGATTTGGAGCTGGCTGTTCCTGGTACATATCGAGCAGAATCTCCGGTGGTGACAATTAGATCATTTGCACGCCAGCTAGTTGTCATAACATCCAAACAGCGACCAAGGAAATTAACAATTCATGGGAGTGATGGTGATGAATATGCCTTCTTGCTGAAGGGACATGAAGACTTGCGCCAAGATGAACGTGTCATGCAGCTTTTTGGTTTGGTGAACACGCTGCTGGAGAATTCTAGAAAAACTCAAGAGAAGGATCTCTCCATTCAACGATATTCTGTCATTCCTTTATCTCCAAACAGTGGACTGATTGGTTGGGTCCCAAATTGTGACACCCTTCACCATCTCATTAGGGAGTACAGGGATGCCAGAAAGATCACTCTTAATCAAGAACACAAATATATGCTCAGTTTTGCTCCGGATTATGATCATTTGCCACTCATAGCTAAAGTGGAAGTATTTGAGTATGCCCTGCACAATACAGAGGGAAATGACCTTTCAAGGGTTCTTTGGCTGAAAAGTCGCACTTCGGAGGTATGGCTGGAGAGGAGAACAAACTATACGAGAAGTTTGGCAGTCATGAGCATGGTTGGTTACCTTCTTGGTCTCGGTGATCGTCACCCAAGCAATCTTATGCTGCACCGCTATAGTGGGAAGATCTTGCACATCGACTTTGGGGACTGCTTTGAAGCGTCTATGAATCGCGAAAAGTTTCCTGAGAAGGTCCCCTTTCGATTGACTAGAATGCTTGTGAAAGCAATGGAGGTTAGTGGTATAGAGGGCAACTTCCGTTCAACTTGTGAAAATGTCATGCAAGTTCTGCGAACACATAAAGATAGTGTTATGGCTATGATGGAGGCCTTTGTTCATGATCCTCTCATCAACTGGCGTCTCTTCAACTTCAATGAAGTTCCACAAGTGGCAATGCTAGCAAATAGTCATGTTCCTCCTGTTGTGGATGCCGAAGAACCGGCTCCAGCTAGAGAGCTTCCCCAACCTCAACGTGGTGCTCGTGAAAGGGAACTTCTTCAGGCTGTTAATCAACTTGGTGATGCTAATGAGGTCTTAAATGAGCGCGCTGTGGTTGTCATGGCTCGGATGAGTAATAAGCTTACTGGGCGTGATTTTTCCACCAGCTCCTCTGTATCATCTAGCTCTATTCAACATGCAGTAGACCACAGCACGCTGATTTCTGGAGATACTCGAGAAGTTGATCATGGTTTATCTGTTAAGCTGCAAGTTCAAAAGTTGATTGGCCAAGCAACTTCTCATGAAAATTTGTGCCAGAATTATGTCGGGTGGTGTCCTTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

2465

Amino Acids

276.77

Weight (kDa)

6.43

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 760 - 928 1.2e-51 Serine/threonine-protein kinase mTOR domain
FAT PF02259 1432 - 1798 4.9e-97 FAT domain
HEAT_ATR PF23593 1813 - 1892 1.4e-10 Serine/threonine-protein kinase ATR-like, HEAT repeats
FRB_dom PF08771 1905 - 2007 5.5e-38 FKBP12-rapamycin binding domain
PI3_PI4_kinase PF00454 2075 - 2324 2.4e-82 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 2435 - 2465 1.8e-14 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2747
AarI CACCTGC 1 cut(s) 4720
AasI GACNNNNNNGTC 1 cut(s) 383
Acc36I ACCTGC 2 cut(s) 3163, 4720
AccB7I CCANNNNNTGG 4 cut(s) 1379, 2497, 3680, 6383
AccBSI CCGCTC 4 cut(s) 2213, 3086, 4693, 5642
AccI GTMKAC 4 cut(s) 1285, 2354, 3421, 7254
AccII CGCG 5 cut(s) 106, 1836, 4491, 6786, 7158
AccIII TCCGGA 2 cut(s) 3243, 6499
AcoI YGGCCR 5 cut(s) 3, 1047, 2522, 4344, 7336
AcuI CTGAAG 8 cut(s) 2562, 3099, 3638, 4674, 4791, 5442, 6260, 7094
AcyI GRCGYC 3 cut(s) 72, 1651, 6970
AflII CTTAAG 1 cut(s) 172
AflIII ACRYGT 5 cut(s) 104, 968, 2720, 5455, 6271
AhdI GACNNNNNGTC 1 cut(s) 6052
AhlI ACTAGT 1 cut(s) 3737
AjiI CACGTC 3 cut(s) 2363, 3131, 3392
AjnI CCWGG 9 cut(s) 387, 1770, 2437, 2875, 4508, 4830, 5131, 5501, 6103
AleI CACNNNNGTG 1 cut(s) 1104
AloI GAACNNNNNNTCC 2 cut(s) 1211, 1243
Alw21I GWGCWC 8 cut(s) 268, 2270, 2398, 3886, 3952, 4753, 5010, 7093
AlwNI CAGNNNCTG 3 cut(s) 1706, 4594, 5312
Ama87I CYCGRG 4 cut(s) 3165, 4871, 5843, 7284
Aor13HI TCCGGA 2 cut(s) 3243, 6499
ApaI GGGCCC 1 cut(s) 4669
ArsI GACNNNNNNTTYG 2 cut(s) 3403, 3435
AseI ATTAAT 1 cut(s) 5466
Asp700I GAANNNNTTC 4 cut(s) 1680, 5661, 6792, 7107
AspLEI GCGC 6 cut(s) 1796, 3265, 3564, 6261, 7158, 7160
AsuC2I CCSGG 3 cut(s) 54, 424, 5723
AsuII TTCGAA 3 cut(s) 723, 3508, 4465
AsuNHI GCTAGC 1 cut(s) 7009
AvaI CYCGRG 4 cut(s) 3165, 4871, 5843, 7284
AvaII GGWCC 5 cut(s) 385, 2593, 4260, 6400, 6805
AxyI CCTNAGG 3 cut(s) 23, 860, 1362
BaeGI GKGCMC 2 cut(s) 3724, 4669
BalI TGGCCA 2 cut(s) 2524, 7338
BanII GRGCYC 4 cut(s) 268, 4669, 5010, 5125
BarI GAAGNNNNNNTAC 4 cut(s) 739, 771, 3984, 4016
BauI CACGAG 1 cut(s) 7092
BbsI GAAGAC 3 cut(s) 4962, 5378, 6258
Bbv12I GWGCWC 8 cut(s) 268, 2270, 2398, 3886, 3952, 4753, 5010, 7093
BbvCI CCTCAGC 1 cut(s) 1413
BceAI ACGGC 2 cut(s) 1638, 3365
BcgI CGANNNNNNTGC 2 cut(s) 2062, 2096
BciT130I CCWGG 9 cut(s) 389, 1772, 2439, 2877, 4510, 4832, 5133, 5503, 6105
BciVI GTATCC 4 cut(s) 95, 2978, 4689, 5499
BclI TGATCA 2 cut(s) 6508, 7294
BcnI CCSGG 3 cut(s) 54, 424, 5723
BcuI ACTAGT 1 cut(s) 3737
BfmI CTRYAG 9 cut(s) 438, 516, 2238, 3861, 3984, 4035, 4988, 5059, 6727
BfrI CTTAAG 1 cut(s) 172
BfuAI ACCTGC 2 cut(s) 3163, 4720
BfuI GTATCC 4 cut(s) 95, 2978, 4689, 5499
BglI GCCNNNNNGGC 1 cut(s) 4301
BglII AGATCT 2 cut(s) 4838, 6738
BlpI GCTNAGC 1 cut(s) 4269
BmcAI AGTACT 2 cut(s) 2370, 5065
Bme18I GGWCC 5 cut(s) 385, 2593, 4260, 6400, 6805
BmeRI GACNNNNNGTC 1 cut(s) 6052
BmeT110I CYCGRG 4 cut(s) 3165, 4871, 5843, 7284
BmgBI CACGTC 3 cut(s) 2363, 3131, 3392
BmrI ACTGGG 4 cut(s) 1994, 2280, 5284, 7206
BmtI GCTAGC 1 cut(s) 7013
BmuI ACTGGG 4 cut(s) 1994, 2280, 5284, 7206
BpiI GAAGAC 3 cut(s) 4962, 5378, 6258
BplI GAGNNNNNCTC 4 cut(s) 4944, 4976, 6044, 6076
BpmI CTGGAG 6 cut(s) 1003, 5939, 6329, 6644, 7041, 7296
Bpu10I CCTNAGC 3 cut(s) 896, 1413, 1776
Bpu1102I GCTNAGC 1 cut(s) 4269
Bpu14I TTCGAA 3 cut(s) 723, 3508, 4465
BpuEI CTTGAG 6 cut(s) 1163, 4112, 4137, 4625, 5628, 6312
BpuMI CCSGG 3 cut(s) 54, 424, 5723
Bsa29I ATCGAT 2 cut(s) 943, 4617
BsaAI YACGTR 3 cut(s) 1106, 1124, 2000
BsaBI GATNNNNATC 2 cut(s) 3972, 6507
BsaHI GRCGYC 3 cut(s) 72, 1651, 6970
BsaI GGTCTC 2 cut(s) 2666, 6692
BsaWI WCCGGW 3 cut(s) 3243, 6127, 6499
BsaXI ACNNNNNCTCC 6 cut(s) 2100, 2130, 2202, 2232, 6202, 6232
Bse118I RCCGGY 2 cut(s) 1049, 7051
Bse21I CCTNAGG 3 cut(s) 23, 860, 1362
Bse3DI GCAATG 9 cut(s) 438, 1185, 2028, 2160, 5007, 5082, 5828, 6846, 7011
Bse8I GATNNNNATC 2 cut(s) 3972, 6507
BseAI TCCGGA 2 cut(s) 3243, 6499
BseBI CCWGG 9 cut(s) 389, 1772, 2439, 2877, 4510, 4832, 5133, 5503, 6105
BseCI ATCGAT 2 cut(s) 943, 4617
BseJI GATNNNNATC 2 cut(s) 3972, 6507
BseMI GCAATG 9 cut(s) 438, 1185, 2028, 2160, 5007, 5082, 5828, 6846, 7011
BsePI GCGCGC 1 cut(s) 7156
BseRI GAGGAG 4 cut(s) 1679, 4622, 6643, 7210
BseSI GKGCMC 2 cut(s) 3724, 4669
BseYI CCCAGC 4 cut(s) 1856, 3545, 4059, 4264
BsgI GTGCAG 8 cut(s) 35, 85, 1469, 1968, 3191, 3621, 6539, 6704
Bsh1236I CGCG 5 cut(s) 106, 1836, 4491, 6786, 7158
BshVI ATCGAT 2 cut(s) 943, 4617
BsiHKAI GWGCWC 8 cut(s) 268, 2270, 2398, 3886, 3952, 4753, 5010, 7093
BsiHKCI CYCGRG 4 cut(s) 3165, 4871, 5843, 7284
BsiSI CCGG 8 cut(s) 54, 423, 1050, 3244, 5722, 6128, 6500, 7052
BslFI GGGAC 6 cut(s) 4, 2606, 6258, 6386, 6773, 6791
BsmBI CGTCTC 3 cut(s) 13, 46, 6977
BsmFI GGGAC 6 cut(s) 4, 2606, 6258, 6386, 6773, 6791
BsmI GAATGC 4 cut(s) 316, 458, 1331, 6831
Bso31I GGTCTC 2 cut(s) 2666, 6692
BsoBI CYCGRG 4 cut(s) 3165, 4871, 5843, 7284
Bsp119I TTCGAA 3 cut(s) 723, 3508, 4465
Bsp120I GGGCCC 1 cut(s) 4665
Bsp13I TCCGGA 2 cut(s) 3243, 6499
Bsp1407I TGTACA 4 cut(s) 700, 1612, 2260, 4625
Bsp1720I GCTNAGC 1 cut(s) 4269
Bsp19I CCATGG 2 cut(s) 4900, 5407
Bsp68I TCGCGA 2 cut(s) 1836, 6786
BspDI ATCGAT 2 cut(s) 943, 4617
BspEI TCCGGA 2 cut(s) 3243, 6499
BspFNI CGCG 5 cut(s) 106, 1836, 4491, 6786, 7158
BspHI TCATGA 6 cut(s) 822, 4882, 5908, 6660, 6949, 7351
BspMAI CTGCAG 4 cut(s) 442, 520, 4039, 4992
BspMI ACCTGC 2 cut(s) 3163, 4720
BspOI GCTAGC 1 cut(s) 7013
BspQI GCTCTTC 2 cut(s) 1905, 4224
BspT104I TTCGAA 3 cut(s) 723, 3508, 4465
BspTI CTTAAG 1 cut(s) 172
BspTNI GGTCTC 2 cut(s) 2666, 6692
BsrBI CCGCTC 4 cut(s) 2213, 3086, 4693, 5642
BsrDI GCAATG 9 cut(s) 438, 1185, 2028, 2160, 5007, 5082, 5828, 6846, 7011
BsrFI RCCGGY 2 cut(s) 1049, 7051
BsrGI TGTACA 4 cut(s) 700, 1612, 2260, 4625
BssAI RCCGGY 2 cut(s) 1049, 7051
BssHII GCGCGC 1 cut(s) 7156
BssNAI GTATAC 1 cut(s) 1286
BssNI GRCGYC 3 cut(s) 72, 1651, 6970
BssSI CACGAG 1 cut(s) 7092
BssT1I CCWWGG 7 cut(s) 117, 664, 3286, 4215, 4900, 5407, 6188
Bst1107I GTATAC 1 cut(s) 1286
Bst2BI CACGAG 1 cut(s) 7092
Bst2UI CCWGG 9 cut(s) 389, 1772, 2439, 2877, 4510, 4832, 5133, 5503, 6105
Bst6I CTCTTC 4 cut(s) 1905, 4224, 4462, 6980
BstACI GRCGYC 3 cut(s) 72, 1651, 6970
BstAFI CTTAAG 1 cut(s) 172
BstAPI GCANNNNNTGC 9 cut(s) 437, 548, 1802, 2434, 3659, 4313, 5282, 5839, 6901
BstAUI TGTACA 4 cut(s) 700, 1612, 2260, 4625
BstBAI YACGTR 3 cut(s) 1106, 1124, 2000
BstBI TTCGAA 3 cut(s) 723, 3508, 4465
BstDSI CCRYGG 2 cut(s) 4900, 5407
BstEII GGTNACC 1 cut(s) 6674
BstENI CCTNNNNNAGG 5 cut(s) 1775, 3245, 3290, 3652, 4247
BstFNI CGCG 5 cut(s) 106, 1836, 4491, 6786, 7158
BstHHI GCGC 6 cut(s) 1796, 3265, 3564, 6261, 7158, 7160
BstNI CCWGG 9 cut(s) 389, 1772, 2439, 2877, 4510, 4832, 5133, 5503, 6105
BstNSI RCATGY 5 cut(s) 972, 2724, 5459, 5812, 7250
BstPI GGTNACC 1 cut(s) 6674
BstSFI CTRYAG 9 cut(s) 438, 516, 2238, 3861, 3984, 4035, 4988, 5059, 6727
BstSLI GKGCMC 2 cut(s) 3724, 4669
BstSNI TACGTA 1 cut(s) 2000
BstUI CGCG 5 cut(s) 106, 1836, 4491, 6786, 7158
BstV2I GAAGAC 3 cut(s) 4962, 5378, 6258
BstX2I RGATCY 7 cut(s) 613, 2128, 2401, 4838, 5986, 6337, 6738
BstXI CCANNNNNNTGG 1 cut(s) 3964
BstYI RGATCY 7 cut(s) 613, 2128, 2401, 4838, 5986, 6337, 6738
BstZ17I GTATAC 1 cut(s) 1286
Bsu15I ATCGAT 2 cut(s) 943, 4617
Bsu36I CCTNAGG 3 cut(s) 23, 860, 1362
BsuI GTATCC 4 cut(s) 95, 2978, 4689, 5499
BsuTUI ATCGAT 2 cut(s) 943, 4617
BtgI CCRYGG 2 cut(s) 4900, 5407
BtgZI GCGATG 3 cut(s) 3326, 4126, 4216
BtrI CACGTC 3 cut(s) 2363, 3131, 3392
BtsI GCAGTG 8 cut(s) 247, 908, 1951, 3540, 3812, 4137, 4404, 4749
BtuMI TCGCGA 2 cut(s) 1836, 6786
BveI ACCTGC 2 cut(s) 3163, 4720
CaiI CAGNNNCTG 3 cut(s) 1706, 4594, 5312
CciI TCATGA 6 cut(s) 822, 4882, 5908, 6660, 6949, 7351
CfoI GCGC 6 cut(s) 1796, 3265, 3564, 6261, 7158, 7160
Cfr10I RCCGGY 2 cut(s) 1049, 7051
ClaI ATCGAT 2 cut(s) 943, 4617
CseI GACGC 6 cut(s) 80, 1659, 2771, 2993, 6762, 6959
CspCI CAANNNNNGTGG 2 cut(s) 1833, 1868
DraI TTTAAA 1 cut(s) 1812
DrdI GACNNNNNNGTC 1 cut(s) 383
DriI GACNNNNNGTC 1 cut(s) 6052
DseDI GACNNNNNNGTC 1 cut(s) 383
EaeI YGGCCR 5 cut(s) 3, 1047, 2522, 4344, 7336
Eam1104I CTCTTC 4 cut(s) 1905, 4224, 4462, 6980
Eam1105I GACNNNNNGTC 1 cut(s) 6052
EarI CTCTTC 4 cut(s) 1905, 4224, 4462, 6980
EciI GGCGGA 2 cut(s) 34, 75
Ecl136II GAGCTC 2 cut(s) 266, 5008
Eco105I TACGTA 1 cut(s) 2000
Eco130I CCWWGG 7 cut(s) 117, 664, 3286, 4215, 4900, 5407, 6188
Eco147I AGGCCT 4 cut(s) 3917, 4133, 5900, 6941
Eco24I GRGCYC 4 cut(s) 268, 4669, 5010, 5125
Eco31I GGTCTC 2 cut(s) 2666, 6692
Eco47I GGWCC 5 cut(s) 385, 2593, 4260, 6400, 6805
Eco53kI GAGCTC 2 cut(s) 266, 5008
Eco57I CTGAAG 8 cut(s) 2562, 3099, 3638, 4674, 4791, 5442, 6260, 7094
Eco81I CCTNAGG 3 cut(s) 23, 860, 1362
Eco88I CYCGRG 4 cut(s) 3165, 4871, 5843, 7284
Eco91I GGTNACC 1 cut(s) 6674
EcoICRI GAGCTC 2 cut(s) 266, 5008
EcoNI CCTNNNNNAGG 5 cut(s) 1775, 3245, 3290, 3652, 4247
EcoO109I RGGNCCY 6 cut(s) 1184, 1261, 2593, 4666, 6400, 6805
EcoO65I GGTNACC 1 cut(s) 6674
EcoRI GAATTC 2 cut(s) 818, 6313
EcoRII CCWGG 9 cut(s) 387, 1770, 2437, 2875, 4508, 4830, 5131, 5501, 6103
EcoT14I CCWWGG 7 cut(s) 117, 664, 3286, 4215, 4900, 5407, 6188
EcoT22I ATGCAT 3 cut(s) 316, 1331, 3072
EcoT38I GRGCYC 4 cut(s) 268, 4669, 5010, 5125
ErhI CCWWGG 7 cut(s) 117, 664, 3286, 4215, 4900, 5407, 6188
Esp3I CGTCTC 3 cut(s) 13, 46, 6977
FaqI GGGAC 6 cut(s) 4, 2606, 6258, 6386, 6773, 6791
FauI CCCGC 3 cut(s) 2138, 3091, 3678
FauNDI CATATG 1 cut(s) 3066
FbaI TGATCA 2 cut(s) 6508, 7294
FblI GTMKAC 4 cut(s) 1285, 2354, 3421, 7254
FriOI GRGCYC 4 cut(s) 268, 4669, 5010, 5125
GlaI GCGC 6 cut(s) 1795, 3264, 3563, 6260, 7157, 7159
GsaI CCCAGC 4 cut(s) 1860, 3549, 4063, 4268
GsuI CTGGAG 6 cut(s) 1003, 5939, 6329, 6644, 7041, 7296
HapII CCGG 8 cut(s) 54, 423, 1050, 3244, 5722, 6128, 6500, 7052
HgaI GACGC 6 cut(s) 80, 1659, 2771, 2993, 6762, 6959
HhaI GCGC 6 cut(s) 1796, 3265, 3564, 6261, 7158, 7160
Hin1I GRCGYC 3 cut(s) 72, 1651, 6970
Hin6I GCGC 6 cut(s) 1794, 3263, 3562, 6259, 7156, 7158
HinP1I GCGC 6 cut(s) 1794, 3263, 3562, 6259, 7156, 7158
HincII GTYRAC 5 cut(s) 1926, 2483, 2841, 3422, 4166
HindII GTYRAC 5 cut(s) 1926, 2483, 2841, 3422, 4166
HindIII AAGCTT 5 cut(s) 64, 1679, 2431, 2756, 7190
HpaI GTTAAC 1 cut(s) 1926
HpaII CCGG 8 cut(s) 54, 423, 1050, 3244, 5722, 6128, 6500, 7052
Hpy99I CGWCG 6 cut(s) 74, 510, 1653, 3393, 3426, 4665
Hsp92I GRCGYC 3 cut(s) 72, 1651, 6970
HspAI GCGC 6 cut(s) 1794, 3263, 3562, 6259, 7156, 7158
KflI GGGWCCC 2 cut(s) 2593, 6400
Kpn2I TCCGGA 2 cut(s) 3243, 6499
Ksp22I TGATCA 2 cut(s) 6508, 7294
KspAI GTTAAC 1 cut(s) 1926
LguI GCTCTTC 2 cut(s) 1905, 4224
MbiI CCGCTC 4 cut(s) 2213, 3086, 4693, 5642
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 7 cut(s) 613, 2128, 2401, 4838, 5986, 6337, 6738
MlsI TGGCCA 2 cut(s) 2524, 7338
MluI ACGCGT 1 cut(s) 104
MluNI TGGCCA 2 cut(s) 2524, 7338
MlyI GAGTC 5 cut(s) 1459, 2055, 4097, 4171, 6062
MmeI TCCRAC 6 cut(s) 772, 1141, 1360, 2608, 4021, 4790
Mox20I TGGCCA 2 cut(s) 2524, 7338
Mph1103I ATGCAT 3 cut(s) 316, 1331, 3072
MroI TCCGGA 2 cut(s) 3243, 6499
MroXI GAANNNNTTC 4 cut(s) 1680, 5661, 6792, 7107
MscI TGGCCA 2 cut(s) 2524, 7338
MslI CAYNNNNRTG 5 cut(s) 1104, 1334, 2451, 6210, 6665
Msp20I TGGCCA 2 cut(s) 2524, 7338
MspA1I CMGCKG 6 cut(s) 1007, 3701, 4268, 4594, 5243, 6025
MspCI CTTAAG 1 cut(s) 172
MspI CCGG 8 cut(s) 54, 423, 1050, 3244, 5722, 6128, 6500, 7052
MunI CAATTG 1 cut(s) 1113
Mva1269I GAATGC 4 cut(s) 316, 458, 1331, 6831
MvaI CCWGG 9 cut(s) 389, 1772, 2439, 2877, 4510, 4832, 5133, 5503, 6105
MvnI CGCG 5 cut(s) 106, 1836, 4491, 6786, 7158
NciI CCSGG 3 cut(s) 54, 424, 5723
NcoI CCATGG 2 cut(s) 4900, 5407
NdeI CATATG 1 cut(s) 3066
NheI GCTAGC 1 cut(s) 7009
NmeAIII GCCGAG 1 cut(s) 5992
NruI TCGCGA 2 cut(s) 1836, 6786
NsiI ATGCAT 3 cut(s) 316, 1331, 3072
NspI RCATGY 5 cut(s) 972, 2724, 5459, 5812, 7250
NspV TTCGAA 3 cut(s) 723, 3508, 4465
OliI CACNNNNGTG 1 cut(s) 1104
PaeI GCATGC 1 cut(s) 5812
PaeR7I CTCGAG 2 cut(s) 5843, 7284
PagI TCATGA 6 cut(s) 822, 4882, 5908, 6660, 6949, 7351
PaqCI CACCTGC 1 cut(s) 4720
PauI GCGCGC 1 cut(s) 7156
PceI AGGCCT 4 cut(s) 3917, 4133, 5900, 6941
PciI ACATGT 3 cut(s) 968, 2720, 5455
PciSI GCTCTTC 2 cut(s) 1905, 4224
PcsI WCGNNNNNNNCGW 5 cut(s) 36, 688, 1129, 1645, 7090
PctI GAATGC 4 cut(s) 316, 458, 1331, 6831
PdmI GAANNNNTTC 4 cut(s) 1680, 5661, 6792, 7107
PfeI GAWTC 9 cut(s) 976, 1517, 2474, 3623, 4642, 5030, 5147, 6122, 6781
PflFI GACNNNGTC 3 cut(s) 1653, 3254, 5568
PflMI CCANNNNNTGG 4 cut(s) 1379, 2497, 3680, 6383
PfoI TCCNGGA 1 cut(s) 5131
PleI GAGTC 5 cut(s) 1459, 2055, 4096, 4170, 6061
PpsI GAGTC 5 cut(s) 1459, 2055, 4096, 4170, 6061
Ppu21I YACGTR 3 cut(s) 1106, 1124, 2000
PpuMI RGGWCCY 3 cut(s) 2593, 6400, 6805
PscI ACATGT 3 cut(s) 968, 2720, 5455
PshBI ATTAAT 1 cut(s) 5466
PsiI TTATAA 1 cut(s) 2747
Psp124BI GAGCTC 2 cut(s) 268, 5010
Psp5II RGGWCCY 3 cut(s) 2593, 6400, 6805
Psp6I CCWGG 9 cut(s) 387, 1770, 2437, 2875, 4508, 4830, 5131, 5501, 6103
PspEI GGTNACC 1 cut(s) 6674
PspFI CCCAGC 4 cut(s) 1856, 3545, 4059, 4264
PspGI CCWGG 9 cut(s) 387, 1770, 2437, 2875, 4508, 4830, 5131, 5501, 6103
PspOMI GGGCCC 1 cut(s) 4665
PspPPI RGGWCCY 3 cut(s) 2593, 6400, 6805
PspXI VCTCGAGB 1 cut(s) 5843
PsrI GAACNNNNNNTAC 4 cut(s) 2361, 2393, 6625, 6657
PstI CTGCAG 4 cut(s) 442, 520, 4039, 4992
PstNI CAGNNNCTG 3 cut(s) 1706, 4594, 5312
PsuI RGATCY 7 cut(s) 613, 2128, 2401, 4838, 5986, 6337, 6738
PsyI GACNNNGTC 3 cut(s) 1653, 3254, 5568
PteI GCGCGC 1 cut(s) 7156
PvuII CAGCTG 6 cut(s) 1007, 3701, 4268, 4594, 5243, 6025
RruI TCGCGA 2 cut(s) 1836, 6786
RseI CAYNNNNRTG 5 cut(s) 1104, 1334, 2451, 6210, 6665
SacI GAGCTC 2 cut(s) 268, 5010
SalI GTCGAC 1 cut(s) 3420
SapI GCTCTTC 2 cut(s) 1905, 4224
ScaI AGTACT 2 cut(s) 2370, 5065
SchI GAGTC 5 cut(s) 1459, 2055, 4097, 4171, 6062
SfcI CTRYAG 9 cut(s) 438, 516, 2238, 3861, 3984, 4035, 4988, 5059, 6727
Sfr274I CTCGAG 2 cut(s) 5843, 7284
SfuI TTCGAA 3 cut(s) 723, 3508, 4465
SinI GGWCC 5 cut(s) 385, 2593, 4260, 6400, 6805
SlaI CTCGAG 2 cut(s) 5843, 7284
SmiMI CAYNNNNRTG 5 cut(s) 1104, 1334, 2451, 6210, 6665
SmlI CTYRAG 9 cut(s) 172, 1142, 4127, 4152, 4604, 5643, 5843, 6327, 7284
SmoI CTYRAG 9 cut(s) 172, 1142, 4127, 4152, 4604, 5643, 5843, 6327, 7284
SnaBI TACGTA 1 cut(s) 2000
SpeI ACTAGT 1 cut(s) 3737
SphI GCATGC 1 cut(s) 5812
SseBI AGGCCT 4 cut(s) 3917, 4133, 5900, 6941
SspI AATATT 5 cut(s) 1168, 1731, 3790, 4047, 5464
SstI GAGCTC 2 cut(s) 268, 5010
StuI AGGCCT 4 cut(s) 3917, 4133, 5900, 6941
StyI CCWWGG 7 cut(s) 117, 664, 3286, 4215, 4900, 5407, 6188
TaqII GACCGA 1 cut(s) 2240
TatI WGTACW 7 cut(s) 700, 1612, 2260, 2368, 4625, 5063, 6438
TauI GCSGC 4 cut(s) 60, 1908, 2216, 5642
TfiI GAWTC 9 cut(s) 976, 1517, 2474, 3623, 4642, 5030, 5147, 6122, 6781
TspGWI ACGGA 8 cut(s) 1563, 2277, 2664, 2809, 2855, 3083, 5726, 6860
Tth111I GACNNNGTC 3 cut(s) 1653, 3254, 5568
Van91I CCANNNNNTGG 4 cut(s) 1379, 2497, 3680, 6383
Vha464I CTTAAG 1 cut(s) 172
VpaK11BI GGWCC 5 cut(s) 385, 2593, 4260, 6400, 6805
VspI ATTAAT 1 cut(s) 5466
XagI CCTNNNNNAGG 5 cut(s) 1775, 3245, 3290, 3652, 4247
XbaI TCTAGA 2 cut(s) 2398, 6317
XceI RCATGY 5 cut(s) 972, 2724, 5459, 5812, 7250
XcmI CCANNNNNNNNNTGG 3 cut(s) 4516, 4946, 7082
XhoI CTCGAG 2 cut(s) 5843, 7284
XmiI GTMKAC 4 cut(s) 1285, 2354, 3421, 7254
XmnI GAANNNNTTC 4 cut(s) 1680, 5661, 6792, 7107
ZrmI AGTACT 2 cut(s) 2370, 5065
Zsp2I ATGCAT 3 cut(s) 316, 1331, 3072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.