FvH4_2g26430

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001325
Physical Location & Seq
Forward (+)
21221114 .. 21227081
5968 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26430.t2

Sequence Viewer

Length: 1350 bp
ATGGATGGAGCAGGGTACCGAGGTACTAACCCTGAACCTAGAAGAAGTAAATCCAGTAGTCCCATTTCTTGTTCAAGTGTGTCTGGTGCTCAGCCAATAGATGATGAACTTGAAGACGAGTCTGTGTCTGAGGTGGGAGATATTGGGGACCGAGCTCTTCACAGCAACAGGAACAGTCCCAGTCACACCTTCAATGACTTGTATGGAGAAGATGTTTTATTGCCATCCTATGGATTTTGGTGTCGTGATAATACTGCATCTCGGACTATCCCCACTGTGCCTTTGTCAGAAGAATTCATATCTCAAACCATTGATGTGGTAGTACGCAATGCAGATAAAAAACAAGAAAATACAAATCAGTTGCCAAAGTGGTTGGAGTATGGTTCATGTATGACACATTTAGCTGTCTTTGGTATTCTTGGGGTCTTGACGAGGTATCTACTGCAAAAACTGTTTGGCCCAGGAGTTTCTGGTGTGACAAGTGACCAAACAATTCTCTACCTTGATCTTCCTTCTAATATGGTTGGTTCTTTCTTGATGGGATGGTGGGGTGTTGTTTTCAAAGCAGATATAGCCTATATATCAGATTTTTTGGCCATTGGATTGTCAACTGGTTATCTAGGAAGTCTTACTACCTTCAGTGGTTGGAACCAGAAAATGCTTGAACTCAGTGTTCAAGGCCATTGGCTTTTTGCTCTGCTTGGCTTTTTAATAGGTTTATTTCTTGCTGCATACTCTATTATTTTTGGGATAGAGACAGCTAAGGGTTTCAGGCAGCTTATTAAAAGGTGTGTAGGGTCTGACATTGCTAGCTCTAGAAAGACCTGGAGGGTTGGCAGTTACAAGCGTCACTTGGCAGTTTGGACAGTGTTGCTGGTGATGCTAGGTTGCCTATGGAGTGTGAGTGGAATCTTGGTGCAGGAGGAGTTCAGAAGTGACAGCAGTGACACACATCTATGGTTGGCCTGCATCGTTGGACCTGTAGGTGTGTGGATCAGGTGGTTCTTAGCTCGATTGAATGGACGTGGATTAGGAAAGACACAGTTGTTGAAATGGGTTCCATTTGGTACTCTAATTGCAAATGTTTCGGCAGCTTGTATCATGGCAGCACTTGCTACTATGAAGAAAGAGGTGAATACCAAGACTTGCGATATCATTGCAAATGGACTGCAACTCGGTTTTTTGGGTTGCCTGAGTACTGTGTCTACCTTCATTTCTGAATTCAATGCAATGAGAGAAAGTAAACACTCTTGGAGAGCATATACATATGCCATGGTTACGATATGTGCCTCGTTTTGTTTGGGGACCCTAATATTTTCATTACCAGTTTGGGCAAAGGGTTACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

450

Amino Acids

49.53

Weight (kDa)

8.23

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRCB PF02537 134 - 251 1.3e-14 CrcB-like protein, Camphor Resistance (CrcB)
CRCB PF02537 321 - 434 8.1e-18 CrcB-like protein, Camphor Resistance (CrcB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 15
AccB1I GGYRCC 1 cut(s) 15
AccB7I CCANNNNNTGG 1 cut(s) 230
AccI GTMKAC 1 cut(s) 1205
AclWI GGATC 1 cut(s) 1001
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 2 cut(s) 293, 1220
AcuI CTGAAG 1 cut(s) 622
AfaI GTAC 5 cut(s) 17, 25, 324, 1069, 1198
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 9 cut(s) 75, 113, 193, 562, 665, 677, 1018, 1051, 1225
AjiI CACGTC 1 cut(s) 1025
AjnI CCWGG 2 cut(s) 460, 824
AluBI AGCT 7 cut(s) 155, 404, 761, 778, 813, 1010, 1094
AluI AGCT 7 cut(s) 155, 404, 761, 778, 813, 1010, 1094
Alw21I GWGCWC 2 cut(s) 91, 157
Alw26I GTCTC 1 cut(s) 749
AlwI GGATC 1 cut(s) 1001
AoxI GGCC 4 cut(s) 457, 594, 679, 963
ApeKI GCWGC 4 cut(s) 728, 775, 1091, 1106
ApoI RAATTY 2 cut(s) 293, 1220
Asp718I GGTACC 1 cut(s) 15
AspS9I GGNCC 4 cut(s) 148, 458, 977, 1305
AsuHPI GGTGA 2 cut(s) 889, 1144
AsuNHI GCTAGC 1 cut(s) 809
AvaII GGWCC 3 cut(s) 148, 977, 1305
BaeI ACNNNNGTAYC 2 cut(s) 7, 40
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 157
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 91, 157
BbvI GCAGC 4 cut(s) 715, 787, 1103, 1118
BccI CCATC 3 cut(s) 232, 532, 537
BcgI CGANNNNNNTGC 4 cut(s) 1068, 1102, 1139, 1173
BciT130I CCWGG 2 cut(s) 462, 826
BcoDI GTCTC 1 cut(s) 749
BfaI CTAG 5 cut(s) 39, 620, 810, 816, 884
BfmI CTRYAG 1 cut(s) 981
BisI GCNGC 4 cut(s) 729, 776, 1092, 1107
BlpI GCTNAGC 1 cut(s) 90
BlsI GCNGC 4 cut(s) 730, 777, 1093, 1108
BmcAI AGTACT 1 cut(s) 1198
Bme1390I CCNGG 2 cut(s) 462, 826
Bme18I GGWCC 3 cut(s) 148, 977, 1305
BmgBI CACGTC 1 cut(s) 1025
BmgT120I GGNCC 4 cut(s) 148, 458, 977, 1305
BmiI GGNNCC 6 cut(s) 17, 149, 650, 1059, 1306, 1307
BmrFI CCNGG 2 cut(s) 462, 826
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 266, 870, 978
BmtI GCTAGC 1 cut(s) 813
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 847
Bpu10I CCTNAGC 1 cut(s) 762
Bpu1102I GCTNAGC 1 cut(s) 90
BsaJI CCNNGG 3 cut(s) 19, 460, 1272
BsaXI ACNNNNNCTCC 4 cut(s) 198, 228, 889, 919
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse1I ACTGG 4 cut(s) 54, 180, 616, 1325
Bse3DI GCAATG 4 cut(s) 334, 804, 1155, 1236
BseBI CCWGG 2 cut(s) 462, 826
BseDI CCNNGG 3 cut(s) 19, 460, 1272
BseGI GGATG 3 cut(s) 10, 224, 548
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMI GCAATG 4 cut(s) 334, 804, 1155, 1236
BseMII CTCAG 4 cut(s) 104, 120, 682, 1184
BseNI ACTGG 4 cut(s) 54, 180, 616, 1325
BseRI GAGGAG 1 cut(s) 938
BseXI GCAGC 4 cut(s) 715, 787, 1103, 1118
BsgI GTGCAG 1 cut(s) 938
BshFI GGCC 4 cut(s) 459, 596, 681, 965
BshNI GGYRCC 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 91, 157
BslFI GGGAC 4 cut(s) 45, 161, 162, 1318
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 749
BsmFI GGGAC 4 cut(s) 45, 161, 162, 1318
BsnI GGCC 4 cut(s) 459, 596, 681, 965
Bsp1286I GDGCHC 2 cut(s) 91, 157
Bsp143I GATC 2 cut(s) 505, 993
Bsp1720I GCTNAGC 1 cut(s) 90
Bsp19I CCATGG 1 cut(s) 1272
BspANI GGCC 4 cut(s) 459, 596, 681, 965
BspCNI CTCAG 4 cut(s) 103, 121, 681, 1185
BspLI GGNNCC 6 cut(s) 17, 149, 650, 1059, 1306, 1307
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 1 cut(s) 1001
BspQI GCTCTTC 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 15
BsrDI GCAATG 4 cut(s) 334, 804, 1155, 1236
BsrI ACTGG 4 cut(s) 54, 180, 616, 1325
BssECI CCNNGG 3 cut(s) 19, 460, 1272
BssMI GATC 2 cut(s) 505, 993
BssT1I CCWWGG 1 cut(s) 1272
Bst2UI CCWGG 2 cut(s) 462, 826
Bst4CI ACNGT 6 cut(s) 176, 277, 453, 868, 1044, 1201
Bst6I CTCTTC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 1112
BstC8I GCNNGC 2 cut(s) 811, 967
BstDEI CTNAG 6 cut(s) 90, 129, 668, 762, 1006, 1193
BstDSI CCRYGG 1 cut(s) 1272
BstF5I GGATG 3 cut(s) 10, 224, 548
BstKTI GATC 2 cut(s) 508, 996
BstMAI GTCTC 1 cut(s) 749
BstMBI GATC 2 cut(s) 505, 993
BstMWI GCNNNNNNNGC 3 cut(s) 572, 880, 1112
BstNI CCWGG 2 cut(s) 462, 826
BstSCI CCNGG 2 cut(s) 460, 824
BstSFI CTRYAG 1 cut(s) 981
BstV1I GCAGC 4 cut(s) 715, 787, 1103, 1118
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 316
BsuRI GGCC 4 cut(s) 459, 596, 681, 965
BtgI CCRYGG 1 cut(s) 1272
BtrI CACGTC 1 cut(s) 1025
BtsCI GGATG 3 cut(s) 10, 224, 548
BtsI GCAGTG 1 cut(s) 949
BtsIMutI CAGTG 5 cut(s) 273, 646, 676, 873, 949
Cac8I GCNNGC 2 cut(s) 811, 967
Cfr13I GGNCC 4 cut(s) 148, 458, 977, 1305
CseI GACGC 1 cut(s) 836
Csp6I GTAC 5 cut(s) 16, 24, 323, 1068, 1197
CviAII CATG 3 cut(s) 387, 1102, 1273
CviQI GTAC 5 cut(s) 16, 24, 323, 1068, 1197
DdeI CTNAG 6 cut(s) 90, 129, 668, 762, 1006, 1193
DpnI GATC 2 cut(s) 507, 995
DpnII GATC 2 cut(s) 505, 993
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Ecl136II GAGCTC 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 1272
Eco24I GRGCYC 1 cut(s) 157
Eco32I GATATC 1 cut(s) 1153
Eco47I GGWCC 3 cut(s) 148, 977, 1305
Eco53kI GAGCTC 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 622
EcoICRI GAGCTC 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 1305
EcoRI GAATTC 2 cut(s) 293, 1220
EcoRII CCWGG 2 cut(s) 460, 824
EcoRV GATATC 1 cut(s) 1153
EcoT14I CCWWGG 1 cut(s) 1272
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 1272
FaeI CATG 3 cut(s) 390, 1105, 1276
FalI AAGNNNNNCTT 2 cut(s) 836, 868
FaqI GGGAC 4 cut(s) 45, 161, 162, 1318
FatI CATG 3 cut(s) 386, 1101, 1272
FauNDI CATATG 1 cut(s) 1267
FblI GTMKAC 1 cut(s) 1205
Fnu4HI GCNGC 4 cut(s) 729, 776, 1092, 1107
FokI GGATG 3 cut(s) 17, 211, 555
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 4 cut(s) 729, 776, 1092, 1107
FspBI CTAG 5 cut(s) 39, 620, 810, 816, 884
GluI GCNGC 4 cut(s) 729, 776, 1092, 1107
GsuI CTGGAG 1 cut(s) 847
HaeIII GGCC 4 cut(s) 459, 596, 681, 965
HgaI GACGC 1 cut(s) 836
Hin1II CATG 3 cut(s) 390, 1105, 1276
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 2 cut(s) 119, 909
HphI GGTGA 2 cut(s) 889, 1144
Hpy166II GTNNAC 3 cut(s) 609, 1206, 1244
Hpy188I TCNGA 7 cut(s) 130, 264, 289, 586, 802, 932, 1219
Hpy188III TCNNGA 4 cut(s) 245, 427, 535, 816
Hpy8I GTNNAC 3 cut(s) 609, 1206, 1244
HpyAV CCTTC 4 cut(s) 199, 522, 646, 1219
HpyCH4III ACNGT 6 cut(s) 176, 277, 453, 868, 1044, 1201
HpyCH4IV ACGT 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 3 cut(s) 572, 880, 1112
HpyF3I CTNAG 6 cut(s) 90, 129, 668, 762, 1006, 1193
HpySE526I ACGT 1 cut(s) 1024
Hsp92II CATG 3 cut(s) 390, 1105, 1276
KflI GGGWCCC 1 cut(s) 1305
KpnI GGTACC 1 cut(s) 19
Kzo9I GATC 2 cut(s) 505, 993
LguI GCTCTTC 1 cut(s) 162
LmnI GCTCC 1 cut(s) 8
Lsp1109I GCAGC 4 cut(s) 715, 787, 1103, 1118
LweI GCATC 3 cut(s) 266, 870, 978
MaeI CTAG 5 cut(s) 39, 620, 810, 816, 884
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 9 cut(s) 182, 475, 482, 839, 848, 935, 944, 1276, 1340
MalI GATC 2 cut(s) 507, 995
MboI GATC 2 cut(s) 505, 993
MboII GAAGA 7 cut(s) 54, 125, 149, 221, 302, 500, 1135
MhlI GDGCHC 2 cut(s) 91, 157
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 4 cut(s) 293, 492, 1074, 1220
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 3 cut(s) 354, 626, 955
MnlI CCTC 7 cut(s) 14, 124, 426, 822, 916, 1123, 1300
Mox20I TGGCCA 1 cut(s) 596
MscI TGGCCA 1 cut(s) 596
MseI TTAA 2 cut(s) 710, 783
MslI CAYNNNNRTG 2 cut(s) 314, 955
Msp20I TGGCCA 1 cut(s) 596
MspR9I CCNGG 2 cut(s) 462, 826
MvaI CCWGG 2 cut(s) 462, 826
MwoI GCNNNNNNNGC 3 cut(s) 572, 880, 1112
NcoI CCATGG 1 cut(s) 1272
NdeI CATATG 1 cut(s) 1267
NdeII GATC 2 cut(s) 505, 993
NheI GCTAGC 1 cut(s) 809
NlaIII CATG 3 cut(s) 390, 1105, 1276
NlaIV GGNNCC 6 cut(s) 17, 149, 650, 1059, 1306, 1307
NmuCI GTSAC 6 cut(s) 182, 475, 482, 848, 935, 944
PciSI GCTCTTC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 909
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 4 cut(s) 730, 777, 1093, 1108
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 1305
Psp124BI GAGCTC 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 1305
Psp6I CCWGG 2 cut(s) 460, 824
PspGI CCWGG 2 cut(s) 460, 824
PspN4I GGNNCC 6 cut(s) 17, 149, 650, 1059, 1306, 1307
PspPI GGNCC 4 cut(s) 148, 458, 977, 1305
PspPPI RGGWCCY 1 cut(s) 1305
RsaI GTAC 5 cut(s) 17, 25, 324, 1069, 1198
RsaNI GTAC 5 cut(s) 16, 24, 323, 1068, 1197
RseI CAYNNNNRTG 2 cut(s) 314, 955
SacI GAGCTC 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 710, 783
SatI GCNGC 4 cut(s) 729, 776, 1092, 1107
Sau3AI GATC 2 cut(s) 505, 993
Sau96I GGNCC 4 cut(s) 148, 458, 977, 1305
ScaI AGTACT 1 cut(s) 1198
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 2 cut(s) 462, 826
SduI GDGCHC 2 cut(s) 91, 157
SfaNI GCATC 3 cut(s) 266, 870, 978
SfcI CTRYAG 1 cut(s) 981
SinI GGWCC 3 cut(s) 148, 977, 1305
SmiMI CAYNNNNRTG 2 cut(s) 314, 955
Sse9I AATT 4 cut(s) 293, 492, 1074, 1220
SspI AATATT 1 cut(s) 1314
SspMI CTAG 5 cut(s) 39, 620, 810, 816, 884
SstI GAGCTC 1 cut(s) 157
StyD4I CCNGG 2 cut(s) 460, 824
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 6 cut(s) 176, 277, 453, 868, 1044, 1201
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 1 cut(s) 1012
TaqII GACCGA 1 cut(s) 165
TasI AATT 4 cut(s) 293, 492, 1074, 1220
TatI WGTACW 1 cut(s) 1196
TfiI GAWTC 1 cut(s) 909
Tru1I TTAA 2 cut(s) 710, 783
Tru9I TTAA 2 cut(s) 710, 783
TscAI CASTG 5 cut(s) 280, 646, 676, 873, 949
TseFI GTSAC 6 cut(s) 182, 475, 482, 848, 935, 944
TseI GCWGC 4 cut(s) 728, 775, 1091, 1106
Tsp45I GTSAC 6 cut(s) 182, 475, 482, 848, 935, 944
TspDTI ATGAA 6 cut(s) 120, 286, 375, 1136, 1201, 1308
TspRI CASTG 5 cut(s) 280, 646, 676, 873, 949
Van91I CCANNNNNTGG 1 cut(s) 230
VpaK11BI GGWCC 3 cut(s) 148, 977, 1305
XapI RAATTY 2 cut(s) 293, 1220
XbaI TCTAGA 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 1205
XspI CTAG 5 cut(s) 39, 620, 810, 816, 884
ZrmI AGTACT 1 cut(s) 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.