pycom08g14420

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001325
Physical Location & Seq
Reverse (-)
13629356 .. 13630398
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g14420.2

Sequence Viewer

Length: 474 bp
ATGATTGAACTCAGTGTTGTAGGCCATTGGGTGTTTGTGCTGCTCCGTTTTCTAATAGCCTACTCCATCATTTTCGGGGTAGAGACAGCAAAGGGTTTTAGGCAGCTTCTTGAGAAGAATTCAGTATGTGGTATCACTAGCTCTAGAAAAAGCTGGAGGGTTGACAGCTACAAGCATCACTTGGTAGCTTTCGCAGTAGAGGAATTTAACAGCGACAGCAGTGAGGCGCAACTGTGGTTGGGTTGCATAGTTGGACCTTTTGGTGTGTGGATCCGGTGGTTCTTAGCCCGGCTTAATGGACGTGGATTAGGGACGACAGGATGCTTAAAATGGGTTCCATATGGTACTCTAATTGCCAATATTTCTGCAGCTTGTGTCATGGCTGCCCTGTCTACTGTGAAGAGAGTGGTGAAAGTTGAGACTTGCGAAAAATTGTTGCAACAGGCATACAATTCGGATTTCTGGGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

158

Amino Acids

17.59

Weight (kDa)

9.08

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 392
AclWI GGATC 2 cut(s) 265, 278
AcsI RAATTY 2 cut(s) 118, 203
AfaI GTAC 1 cut(s) 346
AgsI TTSAA 1 cut(s) 8
AjiI CACGTC 1 cut(s) 302
AluBI AGCT 6 cut(s) 106, 141, 153, 168, 188, 371
AluI AGCT 6 cut(s) 106, 141, 153, 168, 188, 371
Alw26I GTCTC 2 cut(s) 77, 413
AlwI GGATC 2 cut(s) 265, 278
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 4 cut(s) 40, 103, 368, 383
ApoI RAATTY 2 cut(s) 118, 203
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 254
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 421
AvaII GGWCC 1 cut(s) 254
BamHI GGATCC 1 cut(s) 270
BbvI GCAGC 4 cut(s) 27, 115, 370, 380
BccI CCATC 1 cut(s) 74
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 77, 413
BfaI CTAG 2 cut(s) 138, 144
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 4 cut(s) 41, 104, 369, 384
BlsI GCNGC 4 cut(s) 42, 105, 370, 385
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 272, 336
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 2 cut(s) 184, 311
BpmI CTGGAG 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 289
BsaWI WCCGGW 1 cut(s) 273
BseGI GGATG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 25
BseXI GCAGC 4 cut(s) 27, 115, 370, 380
BshFI GGCC 1 cut(s) 24
BsiSI CCGG 2 cut(s) 274, 289
BslFI GGGAC 1 cut(s) 325
BsmAI GTCTC 2 cut(s) 77, 413
BsmFI GGGAC 1 cut(s) 325
BsnI GGCC 1 cut(s) 24
Bsp143I GATC 1 cut(s) 270
BspANI GGCC 1 cut(s) 24
BspCNI CTCAG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 272, 336
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 2 cut(s) 265, 278
BssMI GATC 1 cut(s) 270
Bst4CI ACNGT 2 cut(s) 234, 397
Bst6I CTCTTC 1 cut(s) 395
BstDEI CTNAG 2 cut(s) 11, 283
BstF5I GGATG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 1 cut(s) 273
BstMAI GTCTC 2 cut(s) 77, 413
BstMBI GATC 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 287
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 4 cut(s) 27, 115, 370, 380
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 1 cut(s) 24
BtrI CACGTC 1 cut(s) 302
BtsCI GGATG 1 cut(s) 326
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 2 cut(s) 19, 226
CfoI GCGC 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 345
CviAII CATG 1 cut(s) 379
CviQI GTAC 1 cut(s) 345
DdeI CTNAG 2 cut(s) 11, 283
DpnI GATC 1 cut(s) 272
DpnII GATC 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 254
EcoRI GAATTC 1 cut(s) 118
FaeI CATG 1 cut(s) 382
FaiI YATR 6 cut(s) 127, 248, 340, 342, 380, 448
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FaqI GGGAC 1 cut(s) 325
FatI CATG 1 cut(s) 378
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 4 cut(s) 41, 104, 369, 384
FokI GGATG 1 cut(s) 333
Fsp4HI GCNGC 4 cut(s) 41, 104, 369, 384
FspBI CTAG 2 cut(s) 138, 144
GlaI GCGC 1 cut(s) 228
GluI GCNGC 4 cut(s) 41, 104, 369, 384
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 1 cut(s) 24
HapII CCGG 2 cut(s) 274, 289
HhaI GCGC 1 cut(s) 229
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HpaII CCGG 2 cut(s) 274, 289
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 2 cut(s) 163, 393
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 2 cut(s) 110, 144
Hpy8I GTNNAC 2 cut(s) 163, 393
HpyCH4III ACNGT 2 cut(s) 234, 397
HpyCH4IV ACGT 1 cut(s) 301
HpyCH4V TGCA 3 cut(s) 246, 368, 439
HpyF3I CTNAG 2 cut(s) 11, 283
HpySE526I ACGT 1 cut(s) 301
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 7 cut(s) 139, 287, 302, 303, 401, 428, 448
Lsp1109I GCAGC 4 cut(s) 27, 115, 370, 380
LweI GCATC 2 cut(s) 184, 311
MaeI CTAG 2 cut(s) 138, 144
MaeII ACGT 1 cut(s) 301
MalI GATC 1 cut(s) 272
MboI GATC 1 cut(s) 270
MboII GAAGA 2 cut(s) 127, 412
MflI RGATCY 1 cut(s) 270
MluCI AATT 5 cut(s) 118, 203, 351, 431, 451
MmeI TCCRAC 1 cut(s) 232
MnlI CCTC 3 cut(s) 150, 193, 217
MseI TTAA 3 cut(s) 207, 294, 326
MspI CCGG 2 cut(s) 274, 289
MspR9I CCNGG 1 cut(s) 289
NciI CCSGG 1 cut(s) 289
NdeI CATATG 1 cut(s) 340
NdeII GATC 1 cut(s) 270
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 2 cut(s) 272, 336
PkrI GCNGC 4 cut(s) 42, 105, 370, 385
PspN4I GGNNCC 2 cut(s) 272, 336
PspPI GGNCC 1 cut(s) 254
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 270
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
SaqAI TTAA 3 cut(s) 207, 294, 326
SatI GCNGC 4 cut(s) 41, 104, 369, 384
Sau3AI GATC 1 cut(s) 270
Sau96I GGNCC 1 cut(s) 254
ScrFI CCNGG 1 cut(s) 289
SetI ASST 8 cut(s) 108, 143, 155, 170, 190, 259, 304, 373
SfaNI GCATC 2 cut(s) 184, 311
SfcI CTRYAG 1 cut(s) 366
SinI GGWCC 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 5 cut(s) 118, 203, 351, 431, 451
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 138, 144
StyD4I CCNGG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 234, 397
TaiI ACGT 1 cut(s) 304
TasI AATT 5 cut(s) 118, 203, 351, 431, 451
Tru1I TTAA 3 cut(s) 207, 294, 326
Tru9I TTAA 3 cut(s) 207, 294, 326
TscAI CASTG 2 cut(s) 19, 226
TseI GCWGC 4 cut(s) 40, 103, 368, 383
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 2 cut(s) 19, 226
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 2 cut(s) 118, 203
XbaI TCTAGA 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 392
XspI CTAG 2 cut(s) 138, 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.