pycom15g31750

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001325
Physical Location & Seq
Reverse (-)
30406406 .. 30410954
4549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g31750.8

Sequence Viewer

Length: 1716 bp
ATGAAAAGACAAGTAAAGAACAAACGCCAAACCAAAACATGTGAACAGCGGCCGTGCCGTGCCTCCCAAAACCCCATTCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAGAACCTTCCGACTTCCACTTTTCAATCCCACAATCATCTTTTTTTTCTGCTCATATTTGTTTTTCTGTCTTCCTCCCATTGCGGCCGCCTTCAACCTCTCCAGCCGCCATTCAACACCAAACTCGCCTTTTCGCGTTTCTGCGTTCAAAGAATCTGGGCACCTCTTTCCGCGTTCAAAGAATCAATGTCGCTTCAAGAGAAATCAAGCGCTGTGTTTATGGAGGAAGGGTACCGTGGGACTAATGACACTGAACCCAGACGAAGCGTATCCAATGATCGAACAAGCAGTGCCGCTTCTTCATTAAGGAGGCGGTCTTTAAGTTTATCACATGTTCAGCCTTCTCAAATAGATGATGACAATGAAAGTGAATGTGTGTCAGAGGCAGGAGATATTGGCGATCGGGCCCTTCACAGCAACAGGCACAGTGAGAGTGGCAGCGTCTGCTTGTTTCCTTCAGAGGATGTTCAACTGCAATCCTATGGATTCTGGGGTCGTGACCTTGTTGCATCCAAAGCAATTTCCCCCGTCACTCCCTTGCCAGAGGAAATCATATCTCCTCTTTCCACTGATGCGATCATATCCGAGGACAAAACGCCAGAAAATACAACAAGGTTGCCAAAATTCTTGGAATATGGTTCATGCATGACTCATTTAGCAGTTTTTGGCATTCTTGGGGTCTTAACGAGATATCTTCTGCAAAAATTGTTTGGCCCTGGAGTTGTAGGTGTAACAGGCGACAAAACCATTCTCTACCTTGATCTTCCTTCTAATATGGTTGGCTCTTTCTTGATGGGGTGGTTTGGTGTTGTTTTCAAAGGAGATATATCCTATGTGTCAGATTATTTGGTCATTGGATTATCAACTGGTTACTTGGGAAGCCTTACAACTTTCAGTGGTTGGAACCAGAAAATGGTTGAACTCAGTGTTGAAGGACATTGGGTTTTTGCTCTGCTCGGCTTTTTGATAGGTTTGTTTCTTGCGGCCTACTCCATCATTTTTGGGGTAGAGACAGCGAAGGGTTTTAGGCGGGTTCTTGAGAGGAACTCAGTCTGTGGTATTACCAGCTCTAGAAGGAGGTGGAGGGTTGACAGCTACAAGCGTCACTTGGTAGCTTTAGCAGTGGTGTTGCTGATGCTAAGTTCTCTGTGGAGTATAGGTGGAATACAGTTAAGAGAGGAATTTAACAGTAGCAGCAGCGAGGCGCAACTATGGTTGGGTTGCATAGTTGGACCTTTTGGTGTGTGGATCCGGTGGTTCTTAGCCCGGCTTAATGGACGTGGATTAGGAAAGACGGGTTTGTTGAAATGGGTTCCATTTGGTACTTTAATTGCCAATGTTTCTGCAGCTTGTGTAATGGCAGCCCTGTCTACTGTGAAGAAAGCAGTGAATACTAAGACTTGCGACATTGTTGCAACAGGCATACAATTTGGATTTCTGGGTTGCCTGAGTACTGTTTCTACCTTCATTGCTGAGTTCAATGCGATGAGAGAAAGCAAATACCCTTGGAGAGCATACGCATATGCCATGGCTACGATATGCAGCTCGTTTGGTTTGGGAATCCTGATATATTGTGTACCCGTTTGGACAAGGGCATACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

572

Amino Acids

63.25

Weight (kDa)

9.21

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 342
AccB1I GGYRCC 2 cut(s) 271, 342
AccB7I CCANNNNNTGG 1 cut(s) 1024
AccI GTMKAC 1 cut(s) 1481
AccII CGCG 2 cut(s) 247, 284
AciI CCGC 9 cut(s) 49, 195, 199, 218, 282, 405, 424, 1094, 1141
AclWI GGATC 2 cut(s) 1354, 1367
AcoI YGGCCR 2 cut(s) 50, 196
AcsI RAATTY 2 cut(s) 734, 1292
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 4 cut(s) 344, 1435, 1564, 1689
AfeI AGCGCT 1 cut(s) 322
AfiI CCNNNNNNNGG 1 cut(s) 1024
AflIII ACRYGT 2 cut(s) 38, 442
AjiI CACGTC 1 cut(s) 1391
AjnI CCWGG 1 cut(s) 826
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 5 cut(s) 1179, 1206, 1226, 1460, 1656
AluI AGCT 5 cut(s) 1179, 1206, 1226, 1460, 1656
Alw26I GTCTC 1 cut(s) 1115
AlwI GGATC 2 cut(s) 1354, 1367
AlwNI CAGNNNCTG 1 cut(s) 555
Aor51HI AGCGCT 1 cut(s) 322
AoxI GGCC 5 cut(s) 50, 196, 516, 823, 1095
ApaI GGGCCC 1 cut(s) 520
ApeKI GCWGC 6 cut(s) 549, 1305, 1308, 1457, 1472, 1653
ApoI RAATTY 2 cut(s) 734, 1292
Asp718I GGTACC 1 cut(s) 342
AspLEI GCGC 2 cut(s) 323, 1318
AspS9I GGNCC 4 cut(s) 516, 517, 824, 1343
AsuC2I CCSGG 1 cut(s) 1378
AvaII GGWCC 1 cut(s) 1343
BaeGI GKGCMC 2 cut(s) 274, 520
BamHI GGATCC 1 cut(s) 1359
BanI GGYRCC 2 cut(s) 271, 342
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 6 cut(s) 561, 1317, 1320, 1469, 1484, 1665
BccI CCATC 2 cut(s) 898, 1112
BceAI ACGGC 2 cut(s) 37, 42
BcgI CGANNNNNNTGC 2 cut(s) 1505, 1539
BciT130I CCWGG 1 cut(s) 828
BciVI GTATCC 1 cut(s) 391
BcnI CCSGG 1 cut(s) 1378
BcoDI GTCTC 1 cut(s) 1115
BfaI CTAG 1 cut(s) 1182
BfmI CTRYAG 1 cut(s) 1455
BfoI RGCGCY 1 cut(s) 324
BfuI GTATCC 1 cut(s) 391
BmcAI AGTACT 1 cut(s) 1564
Bme1390I CCNGG 2 cut(s) 828, 1378
Bme18I GGWCC 1 cut(s) 1343
BmgBI CACGTC 1 cut(s) 1391
BmgT120I GGNCC 4 cut(s) 516, 517, 824, 1343
BmiI GGNNCC 6 cut(s) 273, 344, 518, 1016, 1361, 1425
BmrFI CCNGG 2 cut(s) 828, 1378
BmsI GCATC 3 cut(s) 629, 673, 1236
BpiI GAAGAC 1 cut(s) 174
BplI GAGNNNNNCTC 2 cut(s) 1142, 1174
BpmI CTGGAG 2 cut(s) 197, 849
BpuEI CTTGAG 1 cut(s) 1169
BpuMI CCSGG 1 cut(s) 1378
BsaJI CCNNGG 5 cut(s) 346, 696, 826, 1616, 1638
BsaWI WCCGGW 1 cut(s) 1362
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 1255, 1285
Bsc4I CCNNNNNNNGG 1 cut(s) 1024
Bse1I ACTGG 1 cut(s) 982
Bse3DI GCAATG 2 cut(s) 190, 1578
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 5 cut(s) 346, 696, 826, 1616, 1638
BseGI GGATG 2 cut(s) 580, 620
BseLI CCNNNNNNNGG 1 cut(s) 1024
BseMI GCAATG 2 cut(s) 190, 1578
BseMII CTCAG 5 cut(s) 125, 1048, 1173, 1550, 1575
BseNI ACTGG 1 cut(s) 982
BseRI GAGGAG 1 cut(s) 660
BseSI GKGCMC 2 cut(s) 274, 520
BseX3I CGGCCG 2 cut(s) 50, 196
BseXI GCAGC 6 cut(s) 561, 1317, 1320, 1469, 1484, 1665
Bsh1236I CGCG 2 cut(s) 247, 284
Bsh1285I CGRYCG 3 cut(s) 53, 199, 514
BshFI GGCC 5 cut(s) 52, 198, 518, 825, 1097
BshNI GGYRCC 2 cut(s) 271, 342
BsiEI CGRYCG 3 cut(s) 53, 199, 514
BsiSI CCGG 2 cut(s) 1363, 1378
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 1024
BsmAI GTCTC 1 cut(s) 1115
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 780
BsnI GGCC 5 cut(s) 52, 198, 518, 825, 1097
Bsp120I GGGCCC 1 cut(s) 516
Bsp1286I GDGCHC 2 cut(s) 274, 520
Bsp143I GATC 5 cut(s) 388, 511, 687, 871, 1359
Bsp19I CCATGG 1 cut(s) 1638
BspACI CCGC 9 cut(s) 49, 195, 199, 218, 282, 405, 424, 1094, 1141
BspANI GGCC 5 cut(s) 52, 198, 518, 825, 1097
BspCNI CTCAG 5 cut(s) 124, 1047, 1172, 1551, 1576
BspFNI CGCG 2 cut(s) 247, 284
BspLI GGNNCC 6 cut(s) 273, 344, 518, 1016, 1361, 1425
BspMAI CTGCAG 1 cut(s) 1459
BspPI GGATC 2 cut(s) 1354, 1367
BspT107I GGYRCC 2 cut(s) 271, 342
BsrDI GCAATG 2 cut(s) 190, 1578
BsrI ACTGG 1 cut(s) 982
BssECI CCNNGG 5 cut(s) 346, 696, 826, 1616, 1638
BssMI GATC 5 cut(s) 388, 511, 687, 871, 1359
BssT1I CCWWGG 2 cut(s) 1616, 1638
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 6 cut(s) 347, 539, 1281, 1301, 1486, 1567
BstAPI GCANNNNNTGC 1 cut(s) 555
BstDEI CTNAG 8 cut(s) 111, 1034, 1159, 1250, 1372, 1506, 1559, 1584
BstDSI CCRYGG 2 cut(s) 346, 1638
BstF5I GGATG 2 cut(s) 580, 620
BstFNI CGCG 2 cut(s) 247, 284
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 2 cut(s) 323, 1318
BstKTI GATC 5 cut(s) 391, 514, 690, 874, 1362
BstMAI GTCTC 1 cut(s) 1115
BstMBI GATC 5 cut(s) 388, 511, 687, 871, 1359
BstMCI CGRYCG 3 cut(s) 53, 199, 514
BstMWI GCNNNNNNNGC 2 cut(s) 555, 626
BstNI CCWGG 1 cut(s) 828
BstNSI RCATGY 2 cut(s) 42, 446
BstSCI CCNGG 2 cut(s) 826, 1376
BstSFI CTRYAG 1 cut(s) 1455
BstSLI GKGCMC 2 cut(s) 274, 520
BstUI CGCG 2 cut(s) 247, 284
BstV1I GCAGC 6 cut(s) 561, 1317, 1320, 1469, 1484, 1665
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 1359
BstYI RGATCY 1 cut(s) 1359
BstZI CGGCCG 2 cut(s) 50, 196
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 5 cut(s) 52, 198, 518, 825, 1097
BtgI CCRYGG 2 cut(s) 346, 1638
BtgZI GCGATG 1 cut(s) 1610
BtrI CACGTC 1 cut(s) 1391
BtsCI GGATG 2 cut(s) 580, 620
BtsI GCAGTG 3 cut(s) 406, 1239, 1503
BtsIMutI CAGTG 8 cut(s) 360, 406, 544, 678, 1012, 1042, 1239, 1503
CaiI CAGNNNCTG 1 cut(s) 555
CciNI GCGGCCGC 1 cut(s) 196
CfoI GCGC 2 cut(s) 323, 1318
Cfr13I GGNCC 4 cut(s) 516, 517, 824, 1343
CseI GACGC 2 cut(s) 541, 1202
Csp6I GTAC 4 cut(s) 343, 1434, 1563, 1688
CviAII CATG 5 cut(s) 39, 443, 753, 757, 1639
CviQI GTAC 4 cut(s) 343, 1434, 1563, 1688
DdeI CTNAG 8 cut(s) 111, 1034, 1159, 1250, 1372, 1506, 1559, 1584
DpnI GATC 5 cut(s) 390, 513, 689, 873, 1361
DpnII GATC 5 cut(s) 388, 511, 687, 871, 1359
EaeI YGGCCR 2 cut(s) 50, 196
EagI CGGCCG 2 cut(s) 50, 196
EclXI CGGCCG 2 cut(s) 50, 196
Eco130I CCWWGG 2 cut(s) 1616, 1638
Eco24I GRGCYC 1 cut(s) 520
Eco32I GATATC 1 cut(s) 803
Eco47I GGWCC 1 cut(s) 1343
Eco47III AGCGCT 1 cut(s) 322
Eco52I CGGCCG 2 cut(s) 50, 196
Eco57I CTGAAG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 826
EcoRV GATATC 1 cut(s) 803
EcoT14I CCWWGG 2 cut(s) 1616, 1638
EcoT22I ATGCAT 1 cut(s) 758
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 2 cut(s) 1616, 1638
FaeI CATG 5 cut(s) 42, 446, 756, 760, 1642
FalI AAGNNNNNCTT 2 cut(s) 1202, 1234
FaqI GGGAC 1 cut(s) 364
FatI CATG 5 cut(s) 38, 442, 752, 756, 1638
FauI CCCGC 1 cut(s) 1134
FauNDI CATATG 1 cut(s) 1633
FblI GTMKAC 1 cut(s) 1481
FokI GGATG 2 cut(s) 587, 607
FriOI GRGCYC 1 cut(s) 520
FspBI CTAG 1 cut(s) 1182
GlaI GCGC 2 cut(s) 322, 1317
GsuI CTGGAG 2 cut(s) 197, 849
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 5 cut(s) 52, 198, 518, 825, 1097
HapII CCGG 2 cut(s) 1363, 1378
HgaI GACGC 2 cut(s) 541, 1202
HhaI GCGC 2 cut(s) 323, 1318
Hin1II CATG 5 cut(s) 42, 446, 756, 760, 1642
Hin6I GCGC 2 cut(s) 321, 1316
HinP1I GCGC 2 cut(s) 321, 1316
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HinfI GANTC 5 cut(s) 264, 293, 597, 760, 1671
HpaII CCGG 2 cut(s) 1363, 1378
Hpy166II GTNNAC 4 cut(s) 44, 1201, 1482, 1688
Hpy188I TCNGA 6 cut(s) 114, 123, 493, 571, 697, 952
Hpy188III TCNNGA 6 cut(s) 308, 608, 901, 1148, 1182, 1675
Hpy8I GTNNAC 4 cut(s) 44, 1201, 1482, 1688
HpyCH4III ACNGT 6 cut(s) 347, 539, 1281, 1301, 1486, 1567
HpyCH4IV ACGT 1 cut(s) 1390
HpyCH4V TGCA 8 cut(s) 586, 620, 756, 811, 1335, 1457, 1526, 1653
HpyF10VI GCNNNNNNNGC 2 cut(s) 555, 626
HpyF3I CTNAG 8 cut(s) 111, 1034, 1159, 1250, 1372, 1506, 1559, 1584
HpySE526I ACGT 1 cut(s) 1390
Hsp92II CATG 5 cut(s) 42, 446, 756, 760, 1642
HspAI GCGC 2 cut(s) 321, 1316
KpnI GGTACC 1 cut(s) 346
Kzo9I GATC 5 cut(s) 388, 511, 687, 871, 1359
Lsp1109I GCAGC 6 cut(s) 561, 1317, 1320, 1469, 1484, 1665
LweI GCATC 3 cut(s) 629, 673, 1236
MaeI CTAG 1 cut(s) 1182
MaeII ACGT 1 cut(s) 1390
MaeIII GTNAC 5 cut(s) 608, 640, 841, 980, 1214
MalI GATC 5 cut(s) 390, 513, 689, 873, 1361
MboI GATC 5 cut(s) 388, 511, 687, 871, 1359
MboII GAAGA 5 cut(s) 174, 402, 797, 866, 1501
MflI RGATCY 1 cut(s) 1359
MhlI GDGCHC 2 cut(s) 274, 520
MluCI AATT 6 cut(s) 630, 734, 815, 1292, 1440, 1538
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 3 cut(s) 146, 992, 1321
Mph1103I ATGCAT 1 cut(s) 758
MseI TTAA 7 cut(s) 416, 431, 794, 1283, 1296, 1383, 1439
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 2 cut(s) 1363, 1378
MspR9I CCNGG 2 cut(s) 828, 1378
Mva1269I GAATGC 1 cut(s) 780
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 2 cut(s) 247, 284
MwoI GCNNNNNNNGC 2 cut(s) 555, 626
NciI CCSGG 1 cut(s) 1378
NcoI CCATGG 1 cut(s) 1638
NdeI CATATG 1 cut(s) 1633
NdeII GATC 5 cut(s) 388, 511, 687, 871, 1359
NlaIII CATG 5 cut(s) 42, 446, 756, 760, 1642
NlaIV GGNNCC 6 cut(s) 273, 344, 518, 1016, 1361, 1425
NmeAIII GCCGAG 1 cut(s) 1047
NmuCI GTSAC 3 cut(s) 608, 640, 1214
NotI GCGGCCGC 1 cut(s) 196
NsiI ATGCAT 1 cut(s) 758
NspI RCATGY 2 cut(s) 42, 446
PciI ACATGT 2 cut(s) 38, 442
PctI GAATGC 1 cut(s) 780
PfeI GAWTC 4 cut(s) 264, 293, 597, 1671
PflMI CCANNNNNTGG 1 cut(s) 1024
Ple19I CGATCG 1 cut(s) 514
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PscI ACATGT 2 cut(s) 38, 442
Psp6I CCWGG 1 cut(s) 826
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 6 cut(s) 273, 344, 518, 1016, 1361, 1425
PspOMI GGGCCC 1 cut(s) 516
PspPI GGNCC 4 cut(s) 516, 517, 824, 1343
PstI CTGCAG 1 cut(s) 1459
PstNI CAGNNNCTG 1 cut(s) 555
PsuI RGATCY 1 cut(s) 1359
PvuI CGATCG 1 cut(s) 514
RsaI GTAC 4 cut(s) 344, 1435, 1564, 1689
RsaNI GTAC 4 cut(s) 343, 1434, 1563, 1688
SaqAI TTAA 7 cut(s) 416, 431, 794, 1283, 1296, 1383, 1439
Sau3AI GATC 5 cut(s) 388, 511, 687, 871, 1359
Sau96I GGNCC 4 cut(s) 516, 517, 824, 1343
ScaI AGTACT 1 cut(s) 1564
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 2 cut(s) 828, 1378
SduI GDGCHC 2 cut(s) 274, 520
SfaNI GCATC 3 cut(s) 629, 673, 1236
SfcI CTRYAG 1 cut(s) 1455
SinI GGWCC 1 cut(s) 1343
SmlI CTYRAG 1 cut(s) 1148
SmoI CTYRAG 1 cut(s) 1148
Sse9I AATT 6 cut(s) 630, 734, 815, 1292, 1440, 1538
SsiI CCGC 9 cut(s) 49, 195, 199, 218, 282, 405, 424, 1094, 1141
SspMI CTAG 1 cut(s) 1182
StyD4I CCNGG 2 cut(s) 826, 1376
StyI CCWWGG 2 cut(s) 1616, 1638
TaaI ACNGT 6 cut(s) 347, 539, 1281, 1301, 1486, 1567
TaiI ACGT 1 cut(s) 1393
TaqI TCGA 1 cut(s) 391
TasI AATT 6 cut(s) 630, 734, 815, 1292, 1440, 1538
TatI WGTACW 1 cut(s) 1562
TauI GCSGC 6 cut(s) 52, 198, 201, 220, 407, 1097
TfiI GAWTC 4 cut(s) 264, 293, 597, 1671
Tru1I TTAA 7 cut(s) 416, 431, 794, 1283, 1296, 1383, 1439
Tru9I TTAA 7 cut(s) 416, 431, 794, 1283, 1296, 1383, 1439
TscAI CASTG 8 cut(s) 367, 406, 544, 685, 1012, 1042, 1239, 1503
TseFI GTSAC 3 cut(s) 608, 640, 1214
TseI GCWGC 6 cut(s) 549, 1305, 1308, 1457, 1472, 1653
Tsp45I GTSAC 3 cut(s) 608, 640, 1214
TspDTI ATGAA 6 cut(s) 17, 68, 402, 489, 741, 1567
TspRI CASTG 8 cut(s) 367, 406, 544, 685, 1012, 1042, 1239, 1503
Van91I CCANNNNNTGG 1 cut(s) 1024
VpaK11BI GGWCC 1 cut(s) 1343
XapI RAATTY 2 cut(s) 734, 1292
XbaI TCTAGA 1 cut(s) 1181
XceI RCATGY 2 cut(s) 42, 446
XmiI GTMKAC 1 cut(s) 1481
XspI CTAG 1 cut(s) 1182
ZrmI AGTACT 1 cut(s) 1564
Zsp2I ATGCAT 1 cut(s) 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.