Rw6G031610

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001325
Physical Location & Seq
Forward (+)
55704849 .. 55709971
5123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031610.1

Sequence Viewer

Length: 1374 bp
ATGCATCCATTGCCGCCATCCTCAGATAGAAGCTTGCAAACCCTTTTGAATCAGCCACCACGAGAGAAACAAAGCCCTGTGATTATGGAGGGAGCAGGGTACCGAGGTACTGACCCTGAACCTGGAAGTAGTGTACCCAGCAGTGCCAATTCTTCTTCAAAAATGTGTTCTTCAAGTGTATCAGGTGCTCAGCCTTCTGGAATAGATGATGACCCTGATGAGTGTGTGTCTGAGGTGGGAGATATTGGGGATCGAGCTCTGCACAGCAACAGGAACAGTTTCAGTGAGAGCCTTAACAACTTGTCTGGAGAGGATGTTTTATTGCCATCCTATGGGTTTTGGTGTCGTGATCGTACTGCATCCCGTGCTATCCCCACTGTGCCTTTGCCAGAGGAAATCATATCTCTTTCCATTGATGTGGCATCACGCAATGAAGATAAAAAGCAAGTGAGCTTTCGGTTGGAAAATACAAATAAGTTGCTGCCAAAGTGGATGGAGTATGGTTCATGTATGACACATTTAGCCGTTTTTGGTATTCTTGGGGTCTTGACGAGGTATCTACTGCAAAAATTGTTTGGCCCAGGAGGTGCTGGTGTGACAAGTGACCAAACAATTCTTTACCTTGATCTTCCTTCTAATATGGTTGGTTCTTTCTTGATGGGATGGTGGGGTGTTGTTTTCAAAGCAGATATATCCTATGTGTCAGATTTTTTGGCCATTGGATTGTCAACCGGTTATCTGGGAAGCCTTACTACTTTCAGTGGTTGGAACCAGAAAATGCTTGAACTCAGTGTTCAAGGCCATTGGCCTTTTGCTGTGCTTGGCAATTTAATAGGTTTATTTCTTGCGGCATACTCTATTATTTTTGGGATAGAGACATCCAAGGGTTTCAGGCAGCTTCTTAAAAGGTGTTCAGGGTCTGACATTGCCATCTCTAGAAAGACCTGGAGGGTTGACAGCTACAAGCGCCACTTGGCAGTGTGGGCAGTGTTGCTGGTGATGCTAGGTTGTCTATGGAGTGTGAGTGGAATCTTGGTGAAGGAGGACTTTAGAAATGACAACAGTGAGACACATCTATGGTTGGCCTGCATCATTGGACCTGTAGGTGTGTGGATCAGGTGGTTCTTAGCTCGACTCAATGGACGTGGATTAGGAAAGACAGGGTTGTTAAAATGGCTTCCATTTGGTACTCTAATTGCAAATGTTTCTGCAGCTTGTATCATGGCAGCCCTTGCTACTGTGGAGAAAGAGGTAAGGTCTTTTAATCTGATTTTTGTTCAGACCTGCACCGAGACTATACCTCGTTACTTTTTTTCCCGCCATTCTGTCATACTTGAAAAGCTTACAGTTTCACATATATTTGTCACATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

457

Amino Acids

50.29

Weight (kDa)

7.57

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRCB PF02537 174 - 290 4.8e-14 CrcB-like protein, Camphor Resistance (CrcB)
CRCB PF02537 361 - 421 6.1e-07 CrcB-like protein, Camphor Resistance (CrcB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1372
Acc36I ACCTGC 1 cut(s) 1292
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 332
AciI CCGC 3 cut(s) 14, 848, 1318
AclWI GGATC 2 cut(s) 258, 1121
AcoI YGGCCR 1 cut(s) 714
AfaI GTAC 5 cut(s) 101, 109, 135, 355, 1189
AfiI CCNNNNNNNGG 2 cut(s) 122, 332
AgeI ACCGGT 1 cut(s) 731
AgsI TTSAA 7 cut(s) 49, 159, 174, 682, 785, 797, 1337
AjiI CACGTC 1 cut(s) 1145
AjnI CCWGG 3 cut(s) 121, 580, 944
AluBI AGCT 8 cut(s) 33, 257, 453, 898, 960, 1130, 1214, 1342
AluI AGCT 8 cut(s) 33, 257, 453, 898, 960, 1130, 1214, 1342
Alw21I GWGCWC 2 cut(s) 190, 259
Alw26I GTCTC 3 cut(s) 869, 1061, 1286
AlwI GGATC 2 cut(s) 258, 1121
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 5 cut(s) 577, 714, 799, 806, 1083
ApeKI GCWGC 4 cut(s) 481, 895, 1211, 1226
AsiGI ACCGGT 1 cut(s) 731
Asp700I GAANNNNTTC 1 cut(s) 278
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 969
AspS9I GGNCC 2 cut(s) 578, 1097
AsuHPI GGTGA 2 cut(s) 1009, 1048
AvaII GGWCC 1 cut(s) 1097
BaeI ACNNNNGTAYC 2 cut(s) 91, 124
BalI TGGCCA 1 cut(s) 716
BanI GGYRCC 1 cut(s) 99
BanII GRGCYC 1 cut(s) 259
BarI GAAGNNNNNNTAC 2 cut(s) 736, 768
BauI CACGAG 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 190, 259
BbvI GCAGC 4 cut(s) 468, 907, 1223, 1238
BccI CCATC 6 cut(s) 25, 334, 487, 652, 657, 938
BceAI ACGGC 1 cut(s) 509
BciT130I CCWGG 3 cut(s) 123, 582, 946
BcoDI GTCTC 3 cut(s) 869, 1061, 1286
BfaI CTAG 2 cut(s) 936, 1004
BfmI CTRYAG 2 cut(s) 1101, 1209
BfoI RGCGCY 1 cut(s) 970
BfuAI ACCTGC 1 cut(s) 1292
BisI GCNGC 6 cut(s) 14, 482, 849, 896, 1212, 1227
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 6 cut(s) 15, 483, 850, 897, 1213, 1228
Bme1390I CCNGG 3 cut(s) 123, 582, 946
Bme18I GGWCC 1 cut(s) 1097
BmgBI CACGTC 1 cut(s) 1145
BmgT120I GGNCC 2 cut(s) 578, 1097
BmiI GGNNCC 2 cut(s) 101, 770
BmrFI CCNGG 3 cut(s) 123, 582, 946
BmsI GCATC 5 cut(s) 13, 368, 431, 990, 1098
BpmI CTGGAG 2 cut(s) 327, 967
Bpu1102I GCTNAGC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 103, 580, 882
BsaWI WCCGGW 1 cut(s) 731
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 1009, 1039
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 332
Bse118I RCCGGY 1 cut(s) 731
Bse3DI GCAATG 3 cut(s) 8, 436, 924
BseBI CCWGG 3 cut(s) 123, 582, 946
BseDI CCNNGG 3 cut(s) 103, 580, 882
BseGI GGATG 8 cut(s) 4, 17, 319, 326, 359, 498, 668, 878
BseLI CCNNNNNNNGG 2 cut(s) 122, 332
BseMI GCAATG 3 cut(s) 8, 436, 924
BseMII CTCAG 4 cut(s) 36, 203, 222, 802
BseXI GCAGC 4 cut(s) 468, 907, 1223, 1238
BseYI CCCAGC 1 cut(s) 137
BsgI GTGCAG 2 cut(s) 245, 1270
BshFI GGCC 5 cut(s) 579, 716, 801, 808, 1085
BshNI GGYRCC 1 cut(s) 99
BshTI ACCGGT 1 cut(s) 731
BsiHKAI GWGCWC 2 cut(s) 190, 259
BsiSI CCGG 1 cut(s) 732
BslI CCNNNNNNNGG 2 cut(s) 122, 332
BsmAI GTCTC 3 cut(s) 869, 1061, 1286
BsnI GGCC 5 cut(s) 579, 716, 801, 808, 1085
Bsp1286I GDGCHC 2 cut(s) 190, 259
Bsp143I GATC 4 cut(s) 250, 349, 625, 1113
Bsp1720I GCTNAGC 1 cut(s) 189
BspACI CCGC 3 cut(s) 14, 848, 1318
BspANI GGCC 5 cut(s) 579, 716, 801, 808, 1085
BspCNI CTCAG 4 cut(s) 35, 202, 223, 801
BspLI GGNNCC 2 cut(s) 101, 770
BspMAI CTGCAG 1 cut(s) 1213
BspMI ACCTGC 1 cut(s) 1292
BspPI GGATC 2 cut(s) 258, 1121
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 3 cut(s) 8, 436, 924
BsrFI RCCGGY 1 cut(s) 731
BssAI RCCGGY 1 cut(s) 731
BssECI CCNNGG 3 cut(s) 103, 580, 882
BssMI GATC 4 cut(s) 250, 349, 625, 1113
BssSI CACGAG 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 882
Bst2BI CACGAG 1 cut(s) 60
Bst2UI CCWGG 3 cut(s) 123, 582, 946
Bst4CI ACNGT 5 cut(s) 278, 379, 1064, 1240, 1348
BstAPI GCANNNNNTGC 3 cut(s) 10, 365, 1232
BstC8I GCNNGC 2 cut(s) 35, 1087
BstDEI CTNAG 5 cut(s) 22, 189, 231, 788, 1126
BstF5I GGATG 8 cut(s) 4, 17, 319, 326, 359, 498, 668, 878
BstH2I RGCGCY 1 cut(s) 970
BstHHI GCGC 1 cut(s) 969
BstKTI GATC 4 cut(s) 253, 352, 628, 1116
BstMAI GTCTC 3 cut(s) 869, 1061, 1286
BstMBI GATC 4 cut(s) 250, 349, 625, 1113
BstMWI GCNNNNNNNGC 6 cut(s) 10, 365, 966, 983, 1000, 1232
BstNI CCWGG 3 cut(s) 123, 582, 946
BstSCI CCNGG 3 cut(s) 121, 580, 944
BstSFI CTRYAG 2 cut(s) 1101, 1209
BstV1I GCAGC 4 cut(s) 468, 907, 1223, 1238
BstXI CCANNNNNNTGG 1 cut(s) 418
BsuRI GGCC 5 cut(s) 579, 716, 801, 808, 1085
BtrI CACGTC 1 cut(s) 1145
BtsCI GGATG 8 cut(s) 4, 17, 319, 326, 359, 498, 668, 878
BtsI GCAGTG 3 cut(s) 148, 984, 993
BtsIMutI CAGTG 8 cut(s) 148, 289, 375, 766, 796, 984, 993, 1069
BveI ACCTGC 1 cut(s) 1292
Cac8I GCNNGC 2 cut(s) 35, 1087
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 1 cut(s) 969
Cfr10I RCCGGY 1 cut(s) 731
Cfr13I GGNCC 2 cut(s) 578, 1097
Csp6I GTAC 5 cut(s) 100, 108, 134, 354, 1188
CspAI ACCGGT 1 cut(s) 731
CspCI CAANNNNNGTGG 2 cut(s) 1126, 1161
CviAII CATG 2 cut(s) 507, 1222
CviQI GTAC 5 cut(s) 100, 108, 134, 354, 1188
DdeI CTNAG 5 cut(s) 22, 189, 231, 788, 1126
DpnI GATC 4 cut(s) 252, 351, 627, 1115
DpnII GATC 4 cut(s) 250, 349, 625, 1113
EaeI YGGCCR 1 cut(s) 714
Ecl136II GAGCTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 882
Eco24I GRGCYC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 1097
Eco53kI GAGCTC 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 257
EcoRII CCWGG 3 cut(s) 121, 580, 944
EcoT14I CCWWGG 1 cut(s) 882
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 2 cut(s) 510, 1225
FalI AAGNNNNNCTT 4 cut(s) 956, 988, 1031, 1063
FatI CATG 2 cut(s) 506, 1221
FauI CCCGC 1 cut(s) 1325
Fnu4HI GCNGC 6 cut(s) 14, 482, 849, 896, 1212, 1227
FokI GGATG 7 cut(s) 4, 313, 326, 346, 505, 675, 865
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 6 cut(s) 14, 482, 849, 896, 1212, 1227
FspBI CTAG 2 cut(s) 936, 1004
GlaI GCGC 1 cut(s) 968
GluI GCNGC 6 cut(s) 14, 482, 849, 896, 1212, 1227
GsaI CCCAGC 1 cut(s) 141
GsuI CTGGAG 2 cut(s) 327, 967
HaeII RGCGCY 1 cut(s) 970
HaeIII GGCC 5 cut(s) 579, 716, 801, 808, 1085
HapII CCGG 1 cut(s) 732
HhaI GCGC 1 cut(s) 969
Hin1II CATG 2 cut(s) 510, 1225
Hin6I GCGC 1 cut(s) 967
HinP1I GCGC 1 cut(s) 967
HincII GTYRAC 2 cut(s) 729, 955
HindII GTYRAC 2 cut(s) 729, 955
HindIII AAGCTT 2 cut(s) 31, 1340
HinfI GANTC 3 cut(s) 49, 1029, 1134
HpaII CCGG 1 cut(s) 732
HphI GGTGA 2 cut(s) 1009, 1048
Hpy166II GTNNAC 3 cut(s) 134, 729, 955
Hpy188I TCNGA 6 cut(s) 25, 232, 706, 922, 1269, 1281
Hpy188III TCNNGA 6 cut(s) 198, 306, 347, 547, 655, 936
Hpy8I GTNNAC 3 cut(s) 134, 729, 955
HpyAV CCTTC 3 cut(s) 204, 642, 1033
HpyCH4III ACNGT 5 cut(s) 278, 379, 1064, 1240, 1348
HpyCH4IV ACGT 1 cut(s) 1144
HpyCH4V TGCA 9 cut(s) 4, 37, 262, 359, 565, 1089, 1199, 1211, 1287
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 365, 966, 983, 1000, 1232
HpyF3I CTNAG 5 cut(s) 22, 189, 231, 788, 1126
HpySE526I ACGT 1 cut(s) 1144
Hsp92II CATG 2 cut(s) 510, 1225
HspAI GCGC 1 cut(s) 967
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 250, 349, 625, 1113
LmnI GCTCC 1 cut(s) 92
Lsp1109I GCAGC 4 cut(s) 468, 907, 1223, 1238
LweI GCATC 5 cut(s) 13, 368, 431, 990, 1098
MaeI CTAG 2 cut(s) 936, 1004
MaeII ACGT 1 cut(s) 1144
MaeIII GTNAC 4 cut(s) 595, 602, 1304, 1363
MalI GATC 4 cut(s) 252, 351, 627, 1115
MboI GATC 4 cut(s) 250, 349, 625, 1113
MboII GAAGA 5 cut(s) 144, 147, 162, 446, 620
MhlI GDGCHC 2 cut(s) 190, 259
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 5 cut(s) 148, 569, 612, 826, 1194
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 1 cut(s) 1128
MmeI TCCRAC 2 cut(s) 441, 746
Mox20I TGGCCA 1 cut(s) 716
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 716
MseI TTAA 5 cut(s) 294, 830, 903, 1169, 1263
MslI CAYNNNNRTG 2 cut(s) 416, 1075
Msp20I TGGCCA 1 cut(s) 716
MspI CCGG 1 cut(s) 732
MspR9I CCNGG 3 cut(s) 123, 582, 946
MvaI CCWGG 3 cut(s) 123, 582, 946
MwoI GCNNNNNNNGC 6 cut(s) 10, 365, 966, 983, 1000, 1232
NdeII GATC 4 cut(s) 250, 349, 625, 1113
NlaIII CATG 2 cut(s) 510, 1225
NlaIV GGNNCC 2 cut(s) 101, 770
NmuCI GTSAC 3 cut(s) 595, 602, 1363
NsiI ATGCAT 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 2 cut(s) 49, 1029
PflMI CCANNNNNTGG 1 cut(s) 332
PinAI ACCGGT 1 cut(s) 731
PkrI GCNGC 6 cut(s) 15, 483, 850, 897, 1213, 1228
PleI GAGTC 1 cut(s) 1128
PpsI GAGTC 1 cut(s) 1128
PsiI TTATAA 1 cut(s) 1372
Psp124BI GAGCTC 1 cut(s) 259
Psp6I CCWGG 3 cut(s) 121, 580, 944
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 3 cut(s) 121, 580, 944
PspN4I GGNNCC 2 cut(s) 101, 770
PspPI GGNCC 2 cut(s) 578, 1097
PstI CTGCAG 1 cut(s) 1213
PstNI CAGNNNCTG 1 cut(s) 920
RsaI GTAC 5 cut(s) 101, 109, 135, 355, 1189
RsaNI GTAC 5 cut(s) 100, 108, 134, 354, 1188
RseI CAYNNNNRTG 2 cut(s) 416, 1075
SacI GAGCTC 1 cut(s) 259
SaqAI TTAA 5 cut(s) 294, 830, 903, 1169, 1263
SatI GCNGC 6 cut(s) 14, 482, 849, 896, 1212, 1227
Sau3AI GATC 4 cut(s) 250, 349, 625, 1113
Sau96I GGNCC 2 cut(s) 578, 1097
SchI GAGTC 1 cut(s) 1128
ScrFI CCNGG 3 cut(s) 123, 582, 946
SduI GDGCHC 2 cut(s) 190, 259
SfaNI GCATC 5 cut(s) 13, 368, 431, 990, 1098
SfcI CTRYAG 2 cut(s) 1101, 1209
SinI GGWCC 1 cut(s) 1097
SmiMI CAYNNNNRTG 2 cut(s) 416, 1075
Sse9I AATT 5 cut(s) 148, 569, 612, 826, 1194
SsiI CCGC 3 cut(s) 14, 848, 1318
SspMI CTAG 2 cut(s) 936, 1004
SstI GAGCTC 1 cut(s) 259
StyD4I CCNGG 3 cut(s) 121, 580, 944
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 5 cut(s) 278, 379, 1064, 1240, 1348
TaiI ACGT 1 cut(s) 1147
TaqI TCGA 2 cut(s) 253, 1132
TasI AATT 5 cut(s) 148, 569, 612, 826, 1194
TauI GCSGC 2 cut(s) 16, 851
TfiI GAWTC 2 cut(s) 49, 1029
Tru1I TTAA 5 cut(s) 294, 830, 903, 1169, 1263
Tru9I TTAA 5 cut(s) 294, 830, 903, 1169, 1263
TscAI CASTG 8 cut(s) 148, 289, 382, 766, 796, 984, 993, 1069
TseFI GTSAC 3 cut(s) 595, 602, 1363
TseI GCWGC 4 cut(s) 481, 895, 1211, 1226
Tsp45I GTSAC 3 cut(s) 595, 602, 1363
TspDTI ATGAA 2 cut(s) 447, 495
TspRI CASTG 8 cut(s) 148, 289, 382, 766, 796, 984, 993, 1069
Van91I CCANNNNNTGG 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 1097
XbaI TCTAGA 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 2 cut(s) 936, 1004
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.