MD15G1354500.v1.1

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001325
Physical Location & Seq
Reverse (-)
42579600 .. 42583294
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1354500.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGGAGGAAGGGTACCGTGGGACAAATGACACTGAACCCAGACGAAGCGTATCCAATGATCGAACAAGCAGTGCCACTTCTTCATTAAGGAGGCGGTCTTTAAGTTTATCACATGTTCAGCCTTCTCAAATAGATGATGACAATGAAAGTGAATGTGTGTCAGAGGCAGGAGATATTGGCGATCGGGCTCTTCACAGCAACAGGCACAGTGAGAGTGGCAGCGTCTGCTTGTTTCCTTCAGAGGATGTTCAACTGCAATCCTATGGATTCTGGGGTCATGACCCTGTTGCATCCAAAGCAATTTCCCCTGTCACTCCCTTGCCAGAGGAAATCATATCTCATCTTTCCACTGATGCGATCATATCCGAGGACAAAAAGCCAGAAAATACAACAAGGTTGCCAAAATTCTTGGAATATGGTTCATGCATGACTCATTTAGCAGTTTTTGGCATTCTTGGGGTCTTATCGAGATATCTTCTGCAAAAATTGTTTGGCCCTGGAGTTGTAGGTGTAACAAGCGACAAAACCATTCTCTACCTTGATCTTCCTTCCAATATGGTTGGCTCTTTCTTGATGGGGTGGTTTGGTGTTGTTTTCAAAGGAGATATATCCTATGTGTCAGATTATTTGGTCATCGGATTATCAACTGGTTACTTGGGCAGCCTTACAACTTTCAGTGGTTGGAACCAGAAAATGATTGAACTCAGTGTTGAAGGACATTGGGCTTTTGCTCTGCTCGGCTTTTTGATAGGTTTGTTTCTTGCGGCCTACTCCCTCATTTTTGGGGTAGAGACAGCAAAGGGTTTTCGGCAGATTCTTGAGAGGAACTCAGTCTGTGGTATTACCAGCTCTAGAAGGAGGTGGAGGGTTGACAGCTACAAGCGTCACTTGGTAGCTTTAGCAGTGTTGTTGCTGATGCTAAGTTCTCTATGGAGTATAGGTGGAATACAGTTAAGAGAGGAATTTAACAGTAGCAGCAGCGAGGCGCAACTATGGTTGGGTTGCATAGTTGGACCTTTTGGTGTGTGGATTCGGTGGTTCTTAGCCCGGCTTAATGGACGTGGATTAGGAAAGACTGGTTTGTTGAAATGGGTTCCATTTGGTACTTTAATTGCCAATGTTTCTGCAGCTTGTGTAATGGCAGCCCTGTCTACTACGAAGAAAGCCGTGAATACTAAGACTTGCGACATTGTTGCAACAGGCATACAATTTGGATTTCTAGGTTGCCTGAGTACTGTTTCTACCTTCATTGCTGAGTTCAACGCAATGAGAGAAAGCAAATACCCTTGGAGAGCATACGCATATGCCATGGCTACGATATGCACCTCGTTTGGTTTGGGAATCCTGATATATTGTGTACCTGTTTGGACAAGGGCATACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

462

Amino Acids

50.71

Weight (kDa)

8.26

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRCB PF02537 146 - 263 5.5e-15 CrcB-like protein, Camphor Resistance (CrcB)
CRCB PF02537 335 - 446 5.4e-16 CrcB-like protein, Camphor Resistance (CrcB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 12
AccI GTMKAC 1 cut(s) 1151
AciI CCGC 2 cut(s) 94, 764
AcsI RAATTY 2 cut(s) 404, 962
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 4 cut(s) 14, 1105, 1234, 1359
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 6 cut(s) 251, 598, 701, 713, 1087, 1261
AjiI CACGTC 1 cut(s) 1061
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 4 cut(s) 849, 876, 896, 1130
AluI AGCT 4 cut(s) 849, 876, 896, 1130
Alw26I GTCTC 1 cut(s) 785
AlwNI CAGNNNCTG 1 cut(s) 225
AoxI GGCC 2 cut(s) 493, 765
ApeKI GCWGC 6 cut(s) 219, 660, 975, 978, 1127, 1142
ApoI RAATTY 2 cut(s) 404, 962
Asp718I GGTACC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 988
AspS9I GGNCC 2 cut(s) 494, 1013
AsuC2I CCSGG 1 cut(s) 1048
AvaII GGWCC 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 1 cut(s) 190
BbvI GCAGC 6 cut(s) 231, 672, 987, 990, 1139, 1154
BccI CCATC 1 cut(s) 568
BceAI ACGGC 1 cut(s) 1151
BcgI CGANNNNNNTGC 2 cut(s) 1175, 1209
BciT130I CCWGG 1 cut(s) 498
BciVI GTATCC 1 cut(s) 61
BcnI CCSGG 1 cut(s) 1048
BcoDI GTCTC 1 cut(s) 785
BfaI CTAG 2 cut(s) 852, 1220
BfmI CTRYAG 1 cut(s) 1125
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 7 cut(s) 220, 661, 765, 976, 979, 1128, 1143
BlsI GCNGC 7 cut(s) 221, 662, 766, 977, 980, 1129, 1144
BmcAI AGTACT 1 cut(s) 1234
Bme1390I CCNGG 2 cut(s) 498, 1048
Bme18I GGWCC 1 cut(s) 1013
BmgBI CACGTC 1 cut(s) 1061
BmgT120I GGNCC 2 cut(s) 494, 1013
BmiI GGNNCC 3 cut(s) 14, 686, 1095
BmrFI CCNGG 2 cut(s) 498, 1048
BmsI GCATC 3 cut(s) 299, 343, 906
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpmI CTGGAG 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 839
BpuMI CCSGG 1 cut(s) 1048
BsaJI CCNNGG 5 cut(s) 16, 366, 496, 1286, 1308
BsaXI ACNNNNNCTCC 3 cut(s) 26, 925, 955
Bse1I ACTGG 2 cut(s) 652, 1081
Bse3DI GCAATG 2 cut(s) 1248, 1272
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 5 cut(s) 16, 366, 496, 1286, 1308
BseGI GGATG 2 cut(s) 250, 290
BseMI GCAATG 2 cut(s) 1248, 1272
BseMII CTCAG 4 cut(s) 718, 843, 1220, 1245
BseNI ACTGG 2 cut(s) 652, 1081
BseXI GCAGC 6 cut(s) 231, 672, 987, 990, 1139, 1154
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 2 cut(s) 495, 767
BshNI GGYRCC 1 cut(s) 12
BsiEI CGRYCG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 1048
BslFI GGGAC 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 785
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 2 cut(s) 495, 767
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 4 cut(s) 58, 181, 357, 541
Bsp19I CCATGG 1 cut(s) 1308
BspACI CCGC 2 cut(s) 94, 764
BspANI GGCC 2 cut(s) 495, 767
BspCNI CTCAG 4 cut(s) 717, 842, 1221, 1246
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 14, 686, 1095
BspMAI CTGCAG 1 cut(s) 1129
BspQI GCTCTTC 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 1248, 1272
BsrI ACTGG 2 cut(s) 652, 1081
BssECI CCNNGG 5 cut(s) 16, 366, 496, 1286, 1308
BssMI GATC 4 cut(s) 58, 181, 357, 541
BssT1I CCWWGG 2 cut(s) 1286, 1308
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 5 cut(s) 17, 209, 951, 971, 1237
Bst6I CTCTTC 1 cut(s) 195
BstAPI GCANNNNNTGC 1 cut(s) 225
BstDEI CTNAG 7 cut(s) 704, 829, 920, 1042, 1176, 1229, 1254
BstDSI CCRYGG 2 cut(s) 16, 1308
BstF5I GGATG 2 cut(s) 250, 290
BstHHI GCGC 1 cut(s) 988
BstKTI GATC 4 cut(s) 61, 184, 360, 544
BstMAI GTCTC 1 cut(s) 785
BstMBI GATC 4 cut(s) 58, 181, 357, 541
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 2 cut(s) 225, 296
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 2 cut(s) 496, 1046
BstSFI CTRYAG 1 cut(s) 1125
BstV1I GCAGC 6 cut(s) 231, 672, 987, 990, 1139, 1154
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 2 cut(s) 495, 767
BtgI CCRYGG 2 cut(s) 16, 1308
BtrI CACGTC 1 cut(s) 1061
BtsCI GGATG 2 cut(s) 250, 290
BtsI GCAGTG 2 cut(s) 76, 909
BtsIMutI CAGTG 7 cut(s) 30, 76, 214, 348, 682, 712, 909
CaiI CAGNNNCTG 1 cut(s) 225
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 988
Cfr13I GGNCC 2 cut(s) 494, 1013
CseI GACGC 2 cut(s) 211, 872
Csp6I GTAC 4 cut(s) 13, 1104, 1233, 1358
CviAII CATG 5 cut(s) 113, 278, 423, 427, 1309
CviQI GTAC 4 cut(s) 13, 1104, 1233, 1358
DdeI CTNAG 7 cut(s) 704, 829, 920, 1042, 1176, 1229, 1254
DpnI GATC 4 cut(s) 60, 183, 359, 543
DpnII GATC 4 cut(s) 58, 181, 357, 541
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 1286, 1308
Eco24I GRGCYC 1 cut(s) 190
Eco32I GATATC 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 1013
Eco57I CTGAAG 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 496
EcoRV GATATC 1 cut(s) 473
EcoT14I CCWWGG 2 cut(s) 1286, 1308
EcoT22I ATGCAT 1 cut(s) 428
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 2 cut(s) 1286, 1308
FaeI CATG 5 cut(s) 116, 281, 426, 430, 1312
FalI AAGNNNNNCTT 2 cut(s) 872, 904
FaqI GGGAC 1 cut(s) 34
FatI CATG 5 cut(s) 112, 277, 422, 426, 1308
FauNDI CATATG 1 cut(s) 1303
FblI GTMKAC 1 cut(s) 1151
Fnu4HI GCNGC 7 cut(s) 220, 661, 765, 976, 979, 1128, 1143
FokI GGATG 2 cut(s) 257, 277
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 7 cut(s) 220, 661, 765, 976, 979, 1128, 1143
FspBI CTAG 2 cut(s) 852, 1220
GlaI GCGC 1 cut(s) 987
GluI GCNGC 7 cut(s) 220, 661, 765, 976, 979, 1128, 1143
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 2 cut(s) 495, 767
HapII CCGG 1 cut(s) 1048
HgaI GACGC 2 cut(s) 211, 872
HhaI GCGC 1 cut(s) 988
Hin1II CATG 5 cut(s) 116, 281, 426, 430, 1312
Hin6I GCGC 1 cut(s) 986
HinP1I GCGC 1 cut(s) 986
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HinfI GANTC 5 cut(s) 267, 430, 814, 1030, 1341
HpaII CCGG 1 cut(s) 1048
Hpy166II GTNNAC 3 cut(s) 871, 1152, 1358
Hpy188I TCNGA 5 cut(s) 163, 241, 367, 622, 638
Hpy188III TCNNGA 6 cut(s) 278, 468, 571, 818, 852, 1345
Hpy8I GTNNAC 3 cut(s) 871, 1152, 1358
HpyAV CCTTC 6 cut(s) 132, 246, 558, 707, 849, 1255
HpyCH4III ACNGT 5 cut(s) 17, 209, 951, 971, 1237
HpyCH4IV ACGT 1 cut(s) 1060
HpyCH4V TGCA 8 cut(s) 256, 290, 426, 481, 1005, 1127, 1196, 1323
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 296
HpyF3I CTNAG 7 cut(s) 704, 829, 920, 1042, 1176, 1229, 1254
HpySE526I ACGT 1 cut(s) 1060
Hsp92II CATG 5 cut(s) 116, 281, 426, 430, 1312
HspAI GCGC 1 cut(s) 986
KpnI GGTACC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 58, 181, 357, 541
LguI GCTCTTC 1 cut(s) 195
Lsp1109I GCAGC 6 cut(s) 231, 672, 987, 990, 1139, 1154
LweI GCATC 3 cut(s) 299, 343, 906
MaeI CTAG 2 cut(s) 852, 1220
MaeII ACGT 1 cut(s) 1060
MaeIII GTNAC 4 cut(s) 310, 511, 650, 884
MalI GATC 4 cut(s) 60, 183, 359, 543
MboI GATC 4 cut(s) 58, 181, 357, 541
MboII GAAGA 5 cut(s) 72, 182, 467, 536, 1171
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 6 cut(s) 300, 404, 485, 962, 1110, 1208
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 2 cut(s) 662, 991
Mph1103I ATGCAT 1 cut(s) 428
MseI TTAA 6 cut(s) 86, 101, 953, 966, 1053, 1109
MspI CCGG 1 cut(s) 1048
MspR9I CCNGG 2 cut(s) 498, 1048
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 2 cut(s) 225, 296
NciI CCSGG 1 cut(s) 1048
NcoI CCATGG 1 cut(s) 1308
NdeI CATATG 1 cut(s) 1303
NdeII GATC 4 cut(s) 58, 181, 357, 541
NlaIII CATG 5 cut(s) 116, 281, 426, 430, 1312
NlaIV GGNNCC 3 cut(s) 14, 686, 1095
NmeAIII GCCGAG 1 cut(s) 717
NmuCI GTSAC 2 cut(s) 310, 884
NsiI ATGCAT 1 cut(s) 428
NspI RCATGY 1 cut(s) 116
PagI TCATGA 1 cut(s) 277
PciI ACATGT 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 195
PctI GAATGC 1 cut(s) 450
PfeI GAWTC 4 cut(s) 267, 814, 1030, 1341
PkrI GCNGC 7 cut(s) 221, 662, 766, 977, 980, 1129, 1144
Ple19I CGATCG 1 cut(s) 184
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
PscI ACATGT 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 14, 686, 1095
PspPI GGNCC 2 cut(s) 494, 1013
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 1 cut(s) 225
PvuI CGATCG 1 cut(s) 184
RsaI GTAC 4 cut(s) 14, 1105, 1234, 1359
RsaNI GTAC 4 cut(s) 13, 1104, 1233, 1358
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 6 cut(s) 86, 101, 953, 966, 1053, 1109
SatI GCNGC 7 cut(s) 220, 661, 765, 976, 979, 1128, 1143
Sau3AI GATC 4 cut(s) 58, 181, 357, 541
Sau96I GGNCC 2 cut(s) 494, 1013
ScaI AGTACT 1 cut(s) 1234
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 498, 1048
SduI GDGCHC 1 cut(s) 190
SfaNI GCATC 3 cut(s) 299, 343, 906
SfcI CTRYAG 1 cut(s) 1125
SinI GGWCC 1 cut(s) 1013
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
Sse9I AATT 6 cut(s) 300, 404, 485, 962, 1110, 1208
SsiI CCGC 2 cut(s) 94, 764
SspMI CTAG 2 cut(s) 852, 1220
StyD4I CCNGG 2 cut(s) 496, 1046
StyI CCWWGG 2 cut(s) 1286, 1308
TaaI ACNGT 5 cut(s) 17, 209, 951, 971, 1237
TaiI ACGT 1 cut(s) 1063
TaqI TCGA 2 cut(s) 61, 467
TasI AATT 6 cut(s) 300, 404, 485, 962, 1110, 1208
TatI WGTACW 1 cut(s) 1232
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 4 cut(s) 267, 814, 1030, 1341
Tru1I TTAA 6 cut(s) 86, 101, 953, 966, 1053, 1109
Tru9I TTAA 6 cut(s) 86, 101, 953, 966, 1053, 1109
TscAI CASTG 7 cut(s) 37, 76, 214, 355, 682, 712, 909
TseFI GTSAC 2 cut(s) 310, 884
TseI GCWGC 6 cut(s) 219, 660, 975, 978, 1127, 1142
Tsp45I GTSAC 2 cut(s) 310, 884
TspDTI ATGAA 4 cut(s) 72, 159, 411, 1237
TspRI CASTG 7 cut(s) 37, 76, 214, 355, 682, 712, 909
VpaK11BI GGWCC 1 cut(s) 1013
XapI RAATTY 2 cut(s) 404, 962
XbaI TCTAGA 1 cut(s) 851
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 1151
XspI CTAG 2 cut(s) 852, 1220
ZrmI AGTACT 1 cut(s) 1234
Zsp2I ATGCAT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.