MD05G1028300.v1.1

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001325
Physical Location & Seq
Reverse (-)
4648136 .. 4649332
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1028300.v1.1.491

Sequence Viewer

Length: 714 bp
ATGTCAGGTTTGTTTCTTGCAGCCTACTTCATCATTTTTGGGGTAGAGACAGCAAAGGGTTTTAGGCAGATTCTTGAGAGGAACTCAGTGTGTGGTTTTACCAGTTCTAGAAGAAGGTGGAGGGTTGATAGCTACAAGCGTCACTTGGTAGCTTTAGCAGTGTTGTTGTTGATGCTAAGTTCTCTGTGGAGTATAAGTGGAATACTATTGAGAGAGGAATTTAACAGTAGCAGCAGTGAGGCGCAACTATGGTTGGGTTGCATAGTTGGACCTTTTGGTGTGTGGATCTGGTGGTTCTTAGCCTGGCTTAATGGACGTAGATTAGGAAAGACGGGTTTGTTGAAATGGGTTCCATTTGGTACTTTAATTGCCAATGTTTCTGAAGCTTGTGTAGTGGCAGCCCTGTCTAATTTGTCATACATTGTCTTGATCCATGCCCACGATTTTTTCCTTTTTAATAGAAAATCTGACGTGCAAAACAATTTGTTTGTGCAGGTGAATACTAAGACTTGCGACATTGTTGCAACAGGCATACCATTTGGGTTTCTGGATTGCCTAAGTACTGTTTCTACCTTCATTGCTAAGTTCAATGCGATGAGAGAAAGCAAATACCCTTGGAGAGCATACGCATATGCCATGGCTACGATATGCATCTCGTTTCGTTTGGGAATCCTGATATACTGTGTACCTGTTTGGACAAGGGCATACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

238

Amino Acids

26.91

Weight (kDa)

9.65

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRCB PF02537 87 - 222 9.1e-11 CrcB-like protein, Camphor Resistance (CrcB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 484
Acc36I ACCTGC 1 cut(s) 484
AclWI GGATC 2 cut(s) 293, 424
AcsI RAATTY 1 cut(s) 218
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 3 cut(s) 361, 562, 687
AgsI TTSAA 2 cut(s) 343, 589
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 1 cut(s) 302
AluBI AGCT 3 cut(s) 132, 152, 386
AluI AGCT 3 cut(s) 132, 152, 386
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 2 cut(s) 293, 424
ApeKI GCWGC 3 cut(s) 20, 231, 398
ApoI RAATTY 1 cut(s) 218
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 508
AvaII GGWCC 1 cut(s) 269
BbvI GCAGC 3 cut(s) 32, 243, 410
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 1 cut(s) 304
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 484
BisI GCNGC 3 cut(s) 21, 232, 399
BlsI GCNGC 3 cut(s) 22, 233, 400
BmcAI AGTACT 1 cut(s) 562
Bme1390I CCNGG 1 cut(s) 304
Bme18I GGWCC 1 cut(s) 269
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 1 cut(s) 304
BmsI GCATC 2 cut(s) 162, 660
BplI GAGNNNNNCTC 2 cut(s) 68, 100
BpuEI CTTGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 650
BsaJI CCNNGG 2 cut(s) 614, 636
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bse1I ACTGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 576
Bse8I GATNNNNATC 1 cut(s) 650
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 614, 636
BseJI GATNNNNATC 1 cut(s) 650
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 102
BseXI GCAGC 3 cut(s) 32, 243, 410
BsgI GTGCAG 1 cut(s) 512
BsmAI GTCTC 1 cut(s) 41
Bsp143I GATC 2 cut(s) 285, 429
Bsp19I CCATGG 1 cut(s) 636
BspCNI CTCAG 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 351
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 2 cut(s) 293, 424
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 614, 636
BssMI GATC 2 cut(s) 285, 429
BssT1I CCWWGG 2 cut(s) 614, 636
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 3 cut(s) 227, 565, 683
BstDEI CTNAG 6 cut(s) 85, 176, 298, 504, 557, 582
BstDSI CCRYGG 1 cut(s) 636
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 2 cut(s) 288, 432
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 2 cut(s) 285, 429
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstV1I GCAGC 3 cut(s) 32, 243, 410
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 636
BtgZI GCGATG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 472
BtsI GCAGTG 2 cut(s) 165, 241
BtsIMutI CAGTG 3 cut(s) 93, 165, 241
BveI ACCTGC 1 cut(s) 484
CfoI GCGC 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 269
CseI GACGC 1 cut(s) 128
Csp6I GTAC 3 cut(s) 360, 561, 686
CviAII CATG 2 cut(s) 434, 637
CviJI RGCY 8 cut(s) 23, 132, 152, 302, 307, 386, 401, 641
CviKI_1 RGCY 8 cut(s) 23, 132, 152, 302, 307, 386, 401, 641
CviQI GTAC 3 cut(s) 360, 561, 686
DdeI CTNAG 6 cut(s) 85, 176, 298, 504, 557, 582
DpnI GATC 2 cut(s) 287, 431
DpnII GATC 2 cut(s) 285, 429
Eco130I CCWWGG 2 cut(s) 614, 636
Eco47I GGWCC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 302
EcoT14I CCWWGG 2 cut(s) 614, 636
EcoT22I ATGCAT 1 cut(s) 653
ErhI CCWWGG 2 cut(s) 614, 636
FaeI CATG 2 cut(s) 437, 640
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FatI CATG 2 cut(s) 433, 636
FauNDI CATATG 1 cut(s) 631
Fnu4HI GCNGC 3 cut(s) 21, 232, 399
Fsp4HI GCNGC 3 cut(s) 21, 232, 399
FspBI CTAG 1 cut(s) 108
GlaI GCGC 1 cut(s) 243
GluI GCNGC 3 cut(s) 21, 232, 399
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 244
Hin1II CATG 2 cut(s) 437, 640
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 2 cut(s) 70, 669
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 1 cut(s) 686
Hpy188I TCNGA 2 cut(s) 382, 469
Hpy188III TCNNGA 5 cut(s) 74, 108, 427, 548, 673
Hpy8I GTNNAC 1 cut(s) 686
HpyAV CCTTC 2 cut(s) 108, 583
HpyCH4III ACNGT 3 cut(s) 227, 565, 683
HpyCH4IV ACGT 2 cut(s) 316, 471
HpyCH4V TGCA 6 cut(s) 20, 261, 475, 493, 524, 651
HpyF3I CTNAG 6 cut(s) 85, 176, 298, 504, 557, 582
HpySE526I ACGT 2 cut(s) 316, 471
Hsp92II CATG 2 cut(s) 437, 640
HspAI GCGC 1 cut(s) 242
Kzo9I GATC 2 cut(s) 285, 429
Lsp1109I GCAGC 3 cut(s) 32, 243, 410
LweI GCATC 2 cut(s) 162, 660
MaeI CTAG 1 cut(s) 108
MaeII ACGT 2 cut(s) 316, 471
MaeIII GTNAC 1 cut(s) 140
MalI GATC 2 cut(s) 287, 431
MboI GATC 2 cut(s) 285, 429
MboII GAAGA 1 cut(s) 123
MflI RGATCY 1 cut(s) 285
MluCI AATT 4 cut(s) 218, 366, 409, 481
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 4 cut(s) 72, 114, 208, 232
Mph1103I ATGCAT 1 cut(s) 653
MseI TTAA 4 cut(s) 222, 309, 365, 456
MspR9I CCNGG 1 cut(s) 304
MvaI CCWGG 1 cut(s) 304
NcoI CCATGG 1 cut(s) 636
NdeI CATATG 1 cut(s) 631
NdeII GATC 2 cut(s) 285, 429
NlaIII CATG 2 cut(s) 437, 640
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 653
PaqCI CACCTGC 1 cut(s) 484
PfeI GAWTC 2 cut(s) 70, 669
PkrI GCNGC 3 cut(s) 22, 233, 400
Psp6I CCWGG 1 cut(s) 302
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 1 cut(s) 269
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 3 cut(s) 361, 562, 687
RsaNI GTAC 3 cut(s) 360, 561, 686
SaqAI TTAA 4 cut(s) 222, 309, 365, 456
SatI GCNGC 3 cut(s) 21, 232, 399
Sau3AI GATC 2 cut(s) 285, 429
Sau96I GGNCC 1 cut(s) 269
ScaI AGTACT 1 cut(s) 562
ScrFI CCNGG 1 cut(s) 304
SfaNI GCATC 2 cut(s) 162, 660
SinI GGWCC 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 4 cut(s) 218, 366, 409, 481
SspMI CTAG 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 302
StyI CCWWGG 2 cut(s) 614, 636
TaaI ACNGT 3 cut(s) 227, 565, 683
TaiI ACGT 2 cut(s) 319, 474
TasI AATT 4 cut(s) 218, 366, 409, 481
TatI WGTACW 1 cut(s) 560
TfiI GAWTC 2 cut(s) 70, 669
Tru1I TTAA 4 cut(s) 222, 309, 365, 456
Tru9I TTAA 4 cut(s) 222, 309, 365, 456
TscAI CASTG 3 cut(s) 93, 165, 241
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 3 cut(s) 20, 231, 398
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 19, 565
TspRI CASTG 3 cut(s) 93, 165, 241
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 1 cut(s) 218
XbaI TCTAGA 1 cut(s) 107
XspI CTAG 1 cut(s) 108
ZrmI AGTACT 1 cut(s) 562
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.