Rw6G017660

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001325
Physical Location & Seq
Reverse (-)
34982540 .. 34985265
2726 bp
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UTR
Exon/CDS
Intron
Rw6G017660.1

Sequence Viewer

Length: 1392 bp
ATGGAGGGGGGAGAGTACAGTGGGACTAATGACTCTGAACTGAGAACAAGTGTACCAATCGATGTGACAAGAAGTGCCAATTCTTCATTAAGGTGGCGCTCTTTAAGTTCATTAGGTGCTCATCCCTCTCAAGTAGATGAAGACATTGAAAGTGATAGTGTGTCAGAGGTGGGAGATATTGGGGATCGAGCTCTTCACAGCAACAGGTACAGTGTAAGTGGCAGTTTCAACTTCTCATCTGCAGAGGATATTCAATTGCAATCCTATGGATTCTGGTGTCATGATCCTACTGCATCCCATGCAATCTCCACGGTGTCGCCTTTGCCAGAGGAAATCATTTCTCCCCTGTCTACTGATGCCATAGTACGCGATGAGGAGAAAAACCAAGCAAGTACAGAAAGAATACCAAAATTGTTGGAGTATGGTTCATGTATGACTCTTCTAGCCTTCTTTGGCATTCTTGGGGTCTTAACGAGATATCTACTGCAAAAATTGTTCGGCCCCGGAGTTGCTGGTGTGACAAGTGACCAAACAATTCTTTACCTTGATCTTCCTTCTAATATGGTTGGCTCTTTCTTGATGGGGTGGTGCGGTGTTGTTTTTAAAGCAGATATATCATATAGGTCAGATTTTTTGGCAATTGGATTGACAACAGGATACCTGGGAAGTGTTACAACTTTTAGTGGTTGGAACCAGAAAATGCTTGAACTGAGTGTCGAAGGCCATTGGGTTTTTGCAGGTCTCGGTTTTTTAATAGGTTTGTTTCTTGCTGCCTACTCCATCATTTTTGGGGTAGAAACATCCAAGTGTTTTAGGCAGCTGCTTGTAAGGAGTTCAGGGTATGGTGTTGCTAGCTCTAGAAAGAGCTGGAGAGTTGACAGCCTCAAGCGTCACTTGGCAGTTTTGGCAGTGTTTCTGCTGTTGCTAGCTGGATTATGGAGTATGAGTGGAATATTGATGAGGCAGGAGTTTAACAGCAACAGCAGTGAGACACAACTTTGGTTGGCTTGCATCATTGGACCTCTAGGAGTCTGGGTCAGGTGGTTCTTAGCTCGACTAAATGGACGTGGATTGGGAAAGACAGGGTTGTTAAAATGGGTTCCCTTTGGCACTCTAATTGCCAATGTTTCTGCATCTTGTATCATGGCGGCACTTGCTACTGTAAAGAGAGAGGTAAATACCAAGAGTAGCGATATTGTTGCAACAGGCATACAATTTGGATTTTTGGGTTGCTTGAGTACTGTATCTACGTTCATTGCCGAGTTTAATGCAATGAGAGAAAGTAAATACCCCTGGAGAGCATACATCTACGCCATAGTCACCATATGCACCTCATTTTGTTTAGGGACCCTGATATATTCTGTACCTGTTTGGGCAAAGGGAGACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

463

Amino Acids

50.6

Weight (kDa)

6.12

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRCB PF02537 147 - 265 8.5e-14 CrcB-like protein, Camphor Resistance (CrcB)
CRCB PF02537 335 - 448 5.4e-18 CrcB-like protein, Camphor Resistance (CrcB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 728
AccI GTMKAC 1 cut(s) 350
AccII CGCG 1 cut(s) 369
AciI CCGC 2 cut(s) 591, 1148
AclWI GGATC 2 cut(s) 192, 278
AfaI GTAC 7 cut(s) 17, 54, 209, 366, 394, 1240, 1365
AgsI TTSAA 4 cut(s) 149, 229, 254, 707
AjiI CACGTC 1 cut(s) 1067
AjnI CCWGG 2 cut(s) 660, 1292
AluBI AGCT 6 cut(s) 191, 820, 855, 867, 929, 1052
AluI AGCT 6 cut(s) 191, 820, 855, 867, 929, 1052
Alw21I GWGCWC 2 cut(s) 121, 193
Alw26I GTCTC 3 cut(s) 746, 983, 1377
AlwI GGATC 2 cut(s) 192, 278
AoxI GGCC 2 cut(s) 499, 721
ApeKI GCWGC 3 cut(s) 770, 817, 820
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 3 cut(s) 500, 1019, 1347
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 1 cut(s) 1312
AsuNHI GCTAGC 2 cut(s) 851, 925
AvaII GGWCC 2 cut(s) 1019, 1347
BaeI ACNNNNGTAYC 2 cut(s) 36, 69
BanII GRGCYC 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 2 cut(s) 121, 193
BbvI GCAGC 3 cut(s) 757, 807, 829
BccI CCATC 2 cut(s) 574, 788
BcgI CGANNNNNNTGC 4 cut(s) 1181, 1215, 1250, 1284
BciT130I CCWGG 2 cut(s) 662, 1294
BciVI GTATCC 1 cut(s) 650
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 3 cut(s) 746, 983, 1377
BfaI CTAG 5 cut(s) 443, 852, 858, 926, 1025
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 728
BfuI GTATCC 1 cut(s) 650
BisI GCNGC 4 cut(s) 771, 818, 821, 1149
BlsI GCNGC 4 cut(s) 772, 819, 822, 1150
BmcAI AGTACT 1 cut(s) 1240
Bme1390I CCNGG 3 cut(s) 504, 662, 1294
Bme18I GGWCC 2 cut(s) 1019, 1347
BmgBI CACGTC 1 cut(s) 1067
BmgT120I GGNCC 3 cut(s) 500, 1019, 1347
BmiI GGNNCC 5 cut(s) 502, 692, 1101, 1348, 1349
BmrFI CCNGG 3 cut(s) 504, 662, 1294
BmsI GCATC 4 cut(s) 302, 346, 1020, 1142
BmtI GCTAGC 2 cut(s) 855, 929
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 2 cut(s) 889, 1315
BpuEI CTTGAG 3 cut(s) 114, 869, 1255
BpuMI CCSGG 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 746
BsaJI CCNNGG 4 cut(s) 309, 502, 661, 1292
BsaXI ACNNNNNCTCC 4 cut(s) 931, 961, 1020, 1050
Bse3DI GCAATG 2 cut(s) 1254, 1278
BseBI CCWGG 2 cut(s) 662, 1294
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 4 cut(s) 309, 502, 661, 1292
BseGI GGATG 3 cut(s) 121, 293, 800
BseMI GCAATG 2 cut(s) 1254, 1278
BseMII CTCAG 2 cut(s) 32, 701
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 3 cut(s) 757, 807, 829
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 2 cut(s) 501, 723
BshVI ATCGAT 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 121, 193
BsiSI CCGG 1 cut(s) 504
BslFI GGGAC 2 cut(s) 37, 1360
BsmAI GTCTC 3 cut(s) 746, 983, 1377
BsmFI GGGAC 2 cut(s) 37, 1360
BsmI GAATGC 1 cut(s) 456
BsnI GGCC 2 cut(s) 501, 723
Bso31I GGTCTC 1 cut(s) 746
Bsp1286I GDGCHC 2 cut(s) 121, 193
Bsp143I GATC 3 cut(s) 184, 283, 547
BspACI CCGC 2 cut(s) 591, 1148
BspANI GGCC 2 cut(s) 501, 723
BspCNI CTCAG 2 cut(s) 33, 702
BspDI ATCGAT 1 cut(s) 60
BspFNI CGCG 1 cut(s) 369
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 5 cut(s) 502, 692, 1101, 1348, 1349
BspMAI CTGCAG 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 728
BspOI GCTAGC 2 cut(s) 855, 929
BspPI GGATC 2 cut(s) 192, 278
BspQI GCTCTTC 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 746
BsrDI GCAATG 2 cut(s) 1254, 1278
BssECI CCNNGG 4 cut(s) 309, 502, 661, 1292
BssMI GATC 3 cut(s) 184, 283, 547
Bst2UI CCWGG 2 cut(s) 662, 1294
Bst4CI ACNGT 5 cut(s) 20, 212, 313, 1162, 1243
Bst6I CTCTTC 2 cut(s) 198, 444
BstAPI GCANNNNNTGC 1 cut(s) 299
BstC8I GCNNGC 3 cut(s) 853, 927, 1009
BstDEI CTNAG 3 cut(s) 41, 710, 1048
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 3 cut(s) 121, 293, 800
BstFNI CGCG 1 cut(s) 369
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 3 cut(s) 187, 286, 550
BstMAI GTCTC 3 cut(s) 746, 983, 1377
BstMBI GATC 3 cut(s) 184, 283, 547
BstMWI GCNNNNNNNGC 3 cut(s) 299, 905, 1154
BstNI CCWGG 2 cut(s) 662, 1294
BstSCI CCNGG 3 cut(s) 502, 660, 1292
BstSFI CTRYAG 1 cut(s) 240
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 3 cut(s) 757, 807, 829
BstV2I GAAGAC 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 60
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 2 cut(s) 501, 723
BsuTUI ATCGAT 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 309
BtgZI GCGATG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 1067
BtsCI GGATG 3 cut(s) 121, 293, 800
BtsI GCAGTG 2 cut(s) 915, 991
BtsIMutI CAGTG 4 cut(s) 25, 217, 915, 991
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 3 cut(s) 853, 927, 1009
CciI TCATGA 1 cut(s) 280
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 3 cut(s) 500, 1019, 1347
ClaI ATCGAT 1 cut(s) 60
CseI GACGC 1 cut(s) 878
Csp6I GTAC 7 cut(s) 16, 53, 208, 365, 393, 1239, 1364
CviAII CATG 4 cut(s) 281, 299, 429, 1144
CviQI GTAC 7 cut(s) 16, 53, 208, 365, 393, 1239, 1364
DdeI CTNAG 3 cut(s) 41, 710, 1048
DpnI GATC 3 cut(s) 186, 285, 549
DpnII GATC 3 cut(s) 184, 283, 547
DraI TTTAAA 1 cut(s) 604
Eam1104I CTCTTC 2 cut(s) 198, 444
EarI CTCTTC 2 cut(s) 198, 444
Ecl136II GAGCTC 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 193
Eco31I GGTCTC 1 cut(s) 746
Eco32I GATATC 1 cut(s) 479
Eco47I GGWCC 2 cut(s) 1019, 1347
Eco53kI GAGCTC 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 1347
EcoRII CCWGG 2 cut(s) 660, 1292
EcoRV GATATC 1 cut(s) 479
EcoT38I GRGCYC 1 cut(s) 193
FaeI CATG 4 cut(s) 284, 302, 432, 1147
FalI AAGNNNNNCTT 2 cut(s) 878, 910
FaqI GGGAC 2 cut(s) 37, 1360
FatI CATG 4 cut(s) 280, 298, 428, 1143
FauNDI CATATG 1 cut(s) 1325
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 4 cut(s) 771, 818, 821, 1149
FokI GGATG 3 cut(s) 108, 280, 787
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 4 cut(s) 771, 818, 821, 1149
FspBI CTAG 5 cut(s) 443, 852, 858, 926, 1025
GlaI GCGC 1 cut(s) 98
GluI GCNGC 4 cut(s) 771, 818, 821, 1149
GsuI CTGGAG 2 cut(s) 889, 1315
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 2 cut(s) 501, 723
HapII CCGG 1 cut(s) 504
HgaI GACGC 1 cut(s) 878
HhaI GCGC 1 cut(s) 99
Hin1II CATG 4 cut(s) 284, 302, 432, 1147
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 4 cut(s) 32, 270, 436, 1029
HpaII CCGG 1 cut(s) 504
HphI GGTGA 1 cut(s) 1312
Hpy166II GTNNAC 3 cut(s) 53, 351, 877
Hpy188I TCNGA 3 cut(s) 37, 166, 628
Hpy188III TCNNGA 3 cut(s) 281, 577, 858
Hpy8I GTNNAC 3 cut(s) 53, 351, 877
HpyAV CCTTC 3 cut(s) 457, 564, 713
HpyCH4III ACNGT 5 cut(s) 20, 212, 313, 1162, 1243
HpyCH4IV ACGT 2 cut(s) 1066, 1250
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 905, 1154
HpyF3I CTNAG 3 cut(s) 41, 710, 1048
HpySE526I ACGT 2 cut(s) 1066, 1250
Hsp92II CATG 4 cut(s) 284, 302, 432, 1147
HspAI GCGC 1 cut(s) 97
KflI GGGWCCC 1 cut(s) 1347
Kzo9I GATC 3 cut(s) 184, 283, 547
LguI GCTCTTC 1 cut(s) 198
Lsp1109I GCAGC 3 cut(s) 757, 807, 829
LweI GCATC 4 cut(s) 302, 346, 1020, 1142
MaeI CTAG 5 cut(s) 443, 852, 858, 926, 1025
MaeII ACGT 2 cut(s) 1066, 1250
MaeIII GTNAC 6 cut(s) 64, 517, 524, 670, 890, 1318
MalI GATC 3 cut(s) 186, 285, 549
MboI GATC 3 cut(s) 184, 283, 547
MboII GAAGA 5 cut(s) 75, 152, 185, 431, 542
MfeI CAATTG 2 cut(s) 254, 639
MhlI GDGCHC 2 cut(s) 121, 193
MluCI AATT 9 cut(s) 79, 254, 410, 491, 534, 639, 1116, 1214, 1387
MlyI GAGTC 3 cut(s) 26, 430, 1038
MmeI TCCRAC 2 cut(s) 396, 668
MseI TTAA 9 cut(s) 89, 104, 470, 603, 752, 972, 1091, 1266, 1390
MslI CAYNNNNRTG 2 cut(s) 91, 805
MspA1I CMGCKG 1 cut(s) 820
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 3 cut(s) 504, 662, 1294
MunI CAATTG 2 cut(s) 254, 639
Mva1269I GAATGC 1 cut(s) 456
MvaI CCWGG 2 cut(s) 662, 1294
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 3 cut(s) 299, 905, 1154
NciI CCSGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 1325
NdeII GATC 3 cut(s) 184, 283, 547
NheI GCTAGC 2 cut(s) 851, 925
NlaIII CATG 4 cut(s) 284, 302, 432, 1147
NlaIV GGNNCC 5 cut(s) 502, 692, 1101, 1348, 1349
NmeAIII GCCGAG 1 cut(s) 1285
NmuCI GTSAC 5 cut(s) 64, 517, 524, 890, 1318
PagI TCATGA 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 198
PctI GAATGC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 270
PkrI GCNGC 4 cut(s) 772, 819, 822, 1150
PleI GAGTC 3 cut(s) 26, 430, 1037
PpsI GAGTC 3 cut(s) 26, 430, 1037
PpuMI RGGWCCY 1 cut(s) 1347
Psp124BI GAGCTC 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 1347
Psp6I CCWGG 2 cut(s) 660, 1292
PspGI CCWGG 2 cut(s) 660, 1292
PspN4I GGNNCC 5 cut(s) 502, 692, 1101, 1348, 1349
PspPI GGNCC 3 cut(s) 500, 1019, 1347
PspPPI RGGWCCY 1 cut(s) 1347
PstI CTGCAG 1 cut(s) 244
PvuII CAGCTG 1 cut(s) 820
RsaI GTAC 7 cut(s) 17, 54, 209, 366, 394, 1240, 1365
RsaNI GTAC 7 cut(s) 16, 53, 208, 365, 393, 1239, 1364
RseI CAYNNNNRTG 2 cut(s) 91, 805
SacI GAGCTC 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 9 cut(s) 89, 104, 470, 603, 752, 972, 1091, 1266, 1390
SatI GCNGC 4 cut(s) 771, 818, 821, 1149
Sau3AI GATC 3 cut(s) 184, 283, 547
Sau96I GGNCC 3 cut(s) 500, 1019, 1347
ScaI AGTACT 1 cut(s) 1240
SchI GAGTC 3 cut(s) 26, 430, 1038
ScrFI CCNGG 3 cut(s) 504, 662, 1294
SduI GDGCHC 2 cut(s) 121, 193
SfaNI GCATC 4 cut(s) 302, 346, 1020, 1142
SfcI CTRYAG 1 cut(s) 240
SinI GGWCC 2 cut(s) 1019, 1347
SmiMI CAYNNNNRTG 2 cut(s) 91, 805
SmlI CTYRAG 3 cut(s) 129, 884, 1234
SmoI CTYRAG 3 cut(s) 129, 884, 1234
Sse9I AATT 9 cut(s) 79, 254, 410, 491, 534, 639, 1116, 1214, 1387
SsiI CCGC 2 cut(s) 591, 1148
SspI AATATT 1 cut(s) 954
SspMI CTAG 5 cut(s) 443, 852, 858, 926, 1025
SstI GAGCTC 1 cut(s) 193
StyD4I CCNGG 3 cut(s) 502, 660, 1292
TaaI ACNGT 5 cut(s) 20, 212, 313, 1162, 1243
TaiI ACGT 2 cut(s) 1069, 1253
TaqI TCGA 4 cut(s) 60, 187, 717, 1054
TasI AATT 9 cut(s) 79, 254, 410, 491, 534, 639, 1116, 1214, 1387
TatI WGTACW 3 cut(s) 15, 392, 1238
TauI GCSGC 1 cut(s) 1151
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 9 cut(s) 89, 104, 470, 603, 752, 972, 1091, 1266, 1390
Tru9I TTAA 9 cut(s) 89, 104, 470, 603, 752, 972, 1091, 1266, 1390
TscAI CASTG 4 cut(s) 25, 217, 915, 991
TseFI GTSAC 5 cut(s) 64, 517, 524, 890, 1318
TseI GCWGC 3 cut(s) 770, 817, 820
Tsp45I GTSAC 5 cut(s) 64, 517, 524, 890, 1318
TspDTI ATGAA 5 cut(s) 75, 99, 153, 417, 1243
TspRI CASTG 4 cut(s) 25, 217, 915, 991
VpaK11BI GGWCC 2 cut(s) 1019, 1347
XbaI TCTAGA 1 cut(s) 857
XmiI GTMKAC 1 cut(s) 350
XspI CTAG 5 cut(s) 443, 852, 858, 926, 1025
ZrmI AGTACT 1 cut(s) 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.