Prupe.1G502900_v2.0.a1

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001325
Physical Location & Seq
Reverse (-)
41583947 .. 41589495
5549 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G502900.7

Sequence Viewer

Length: 1605 bp
ATGGAGGGAGGGTACCGTGGGACTAATGACTCTGAACTCAGACGAAGTGTATCAAATGATCGAACAAGCAGTGCCACGACTTCATTAAGGAGGCGTTCTTTTAGTTTATCAGGTGCTCAGCCTTCCCAAATAGATGATGACATTGAAAGTGAGCGTGTTTCAGAGGCAGGAGATATAGGGGATCGGGCTCTTCACAGCAACAGGCACAGTGAGAGTGGCAGCATCCGCTTGTTGCCTTCAGAGGATATTCAACTGCAATCCTATGGGTTCTGGGGTCGTGATCCGGTTACATCCTCTGCAATATCCCCTGTCTCACCCTTGCCAGAGGAAATCATATCTCCTCTTTCCATTGATGCAGTCGTATGCCATGAGGACAAAAAGCAAGAAACCACAACAAAGTTGCCAAAATTGTTGGAGTATGGTTCATGTATGACTCATTTAGCCGTTTTTGGCATTCTTGGGGTGTTAACGAGATATCTGCTGCAAAAGTTGTTTGGCCCCGGAGTTGTTGGTGTAACAAGCAACCAAACCATTCTCTACCTCGACCTTCCTTCTAATATGGTTGGTTCTTTCTTGATGGGGTGGTTTGGTGTTGTTTTCAAAGGAGATATTTCATATATGTCAGATCATTTGGCCATTGGATTATCAACCGGTTACTTGGGAAGCCTTACAACTTTCAGTGGGTGGAACCAGAAAATGCTTGAACTCAGTGTTGAAGGCCATTGGGTTTTTGTTCTGCTGGGTTTCTTAATAGGTCTGTTTCTTGCGGCCTACTCCATCATTGTTGGGGTAGAGACAGCTAAGGGTTTTAGGAAGCTTCTTGAAAGGAGTTCAGGATGTGGTATAACTAGCTCTGGCACAAGCTGGAGGGTTGACAGCCACAAGCGTCACTTGGTAGTTTTAGCTGTGTTTTCACTGATGCTAATTTCTCTATGGAGTGTAAGTGGAGTATTGCTAAGGGAGGAGTTTAGCAGTGACAGCAGTGAGGCGCAACTATGGTTGGCTTGCATAGTTGGACCTCTAGGTGTGTGGATCAGGTGGTTCTTAGCCAGGCTCAATGGACGTGGATTAGGCAGGATGGGGTTGTTGAAATGGTTTCCATTTGGTACTCTAATTGCCAATGTTTCTGCAGCTTGTGTCATGGCAGCTCTGTCTACTGTGAAGAAAGAGCCTCTTAGCAGAATCCCTGAAGTTCACTGTCTTCTGCGACAAAATTTTGTGTTACCTTCGCCAAGTACACAACTGAGGTTCACTTCTGGGATTTGCCCCGAAGTTCACCATAGAGCCAGATCGTGTGGATTTATTTTACAGTGTTTGGCAGAAATGTCCAAGTCCCCTCCAAACCATGTGGATTTTGTCGTTGCTCATTTATCTACTTTTTTTTCCGTGGATACTAAGACGTGTGATATTGTTGCAACAGGCATACAATTTGGATTGCTGGGCTGCCTGAGTACCGTTTCTACCTTCATTGCTGAGTTCAATGCAATGAGAGAAAGCAAATACCCCTGGAGAGCATACACATATGCCATGGTTACGATATGTACCTCGTTTGGTTTGGGGACCCTGATATATTCTGTACCTGTTTGGACAAAGGGATACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

535

Amino Acids

58.62

Weight (kDa)

8.2

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 12
AccI GTMKAC 1 cut(s) 1154
AciI CCGC 2 cut(s) 226, 767
AclWI GGATC 3 cut(s) 189, 275, 1040
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 1 cut(s) 1213
AcuI CTGAAG 2 cut(s) 222, 1209
AfaI GTAC 6 cut(s) 14, 1108, 1237, 1453, 1543, 1578
AflIII ACRYGT 1 cut(s) 1400
AgeI ACCGGT 1 cut(s) 650
AgsI TTSAA 8 cut(s) 146, 251, 601, 704, 716, 824, 1090, 1480
AjiI CACGTC 2 cut(s) 1064, 1401
AjnI CCWGG 2 cut(s) 1049, 1505
AluBI AGCT 7 cut(s) 800, 817, 852, 864, 905, 1133, 1148
AluI AGCT 7 cut(s) 800, 817, 852, 864, 905, 1133, 1148
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 316, 788
AlwI GGATC 3 cut(s) 189, 275, 1040
AoxI GGCC 4 cut(s) 496, 633, 718, 768
ApeKI GCWGC 5 cut(s) 219, 481, 1130, 1145, 1443
ApoI RAATTY 1 cut(s) 1213
AsiGI ACCGGT 1 cut(s) 650
Asp718I GGTACC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 991
AspS9I GGNCC 3 cut(s) 497, 1016, 1560
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 2 cut(s) 306, 1268
AvaII GGWCC 2 cut(s) 1016, 1560
BalI TGGCCA 1 cut(s) 635
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 1193
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 5 cut(s) 231, 468, 1142, 1157, 1430
BccI CCATC 3 cut(s) 571, 785, 1072
BceAI ACGGC 1 cut(s) 428
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 2 cut(s) 1051, 1507
BciVI GTATCC 2 cut(s) 1384, 1589
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 316, 788
BfaI CTAG 2 cut(s) 849, 1022
BfmI CTRYAG 1 cut(s) 1128
BfuI GTATCC 2 cut(s) 1384, 1589
BisI GCNGC 6 cut(s) 220, 482, 768, 1131, 1146, 1444
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 6 cut(s) 221, 483, 769, 1132, 1147, 1445
Bme1390I CCNGG 3 cut(s) 501, 1051, 1507
Bme18I GGWCC 2 cut(s) 1016, 1560
BmgBI CACGTC 2 cut(s) 1064, 1401
BmgT120I GGNCC 3 cut(s) 497, 1016, 1560
BmiI GGNNCC 5 cut(s) 14, 499, 689, 1561, 1562
BmrFI CCNGG 3 cut(s) 501, 1051, 1507
BmsI GCATC 3 cut(s) 231, 343, 909
BpiI GAAGAC 1 cut(s) 1193
BpmI CTGGAG 2 cut(s) 886, 1528
Bpu10I CCTNAGC 2 cut(s) 801, 956
Bpu1102I GCTNAGC 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 501
BsaJI CCNNGG 5 cut(s) 16, 499, 1386, 1505, 1527
BsaWI WCCGGW 2 cut(s) 283, 650
BsaXI ACNNNNNCTCC 3 cut(s) 26, 928, 958
Bse118I RCCGGY 1 cut(s) 650
Bse3DI GCAATG 2 cut(s) 1467, 1491
BseBI CCWGG 2 cut(s) 1051, 1507
BseDI CCNNGG 5 cut(s) 16, 499, 1386, 1505, 1527
BseGI GGATG 4 cut(s) 222, 290, 842, 1083
BseMI GCAATG 2 cut(s) 1467, 1491
BseMII CTCAG 6 cut(s) 52, 131, 721, 1235, 1439, 1464
BseRI GAGGAG 2 cut(s) 330, 977
BseXI GCAGC 5 cut(s) 231, 468, 1142, 1157, 1430
BseYI CCCAGC 2 cut(s) 739, 1438
BshFI GGCC 4 cut(s) 498, 635, 720, 770
BshNI GGYRCC 1 cut(s) 12
BshTI ACCGGT 1 cut(s) 650
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 3 cut(s) 284, 501, 651
BslFI GGGAC 3 cut(s) 34, 1318, 1573
BsmAI GTCTC 2 cut(s) 316, 788
BsmFI GGGAC 3 cut(s) 34, 1318, 1573
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 4 cut(s) 498, 635, 720, 770
Bsp1286I GDGCHC 2 cut(s) 118, 190
Bsp143I GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
Bsp1720I GCTNAGC 1 cut(s) 117
Bsp19I CCATGG 1 cut(s) 1527
BspACI CCGC 2 cut(s) 226, 767
BspANI GGCC 4 cut(s) 498, 635, 720, 770
BspCNI CTCAG 6 cut(s) 51, 130, 720, 1236, 1440, 1465
BspLI GGNNCC 5 cut(s) 14, 499, 689, 1561, 1562
BspMAI CTGCAG 1 cut(s) 1132
BspPI GGATC 3 cut(s) 189, 275, 1040
BspQI GCTCTTC 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 1467, 1491
BsrFI RCCGGY 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 5 cut(s) 16, 499, 1386, 1505, 1527
BssMI GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
BssT1I CCWWGG 1 cut(s) 1527
Bst2UI CCWGG 2 cut(s) 1051, 1507
Bst4CI ACNGT 6 cut(s) 17, 209, 1159, 1199, 1311, 1456
Bst6I CTCTTC 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 1006
BstDSI CCRYGG 3 cut(s) 16, 1386, 1527
BstF5I GGATG 4 cut(s) 222, 290, 842, 1083
BstHHI GCGC 1 cut(s) 991
BstKTI GATC 6 cut(s) 61, 184, 283, 628, 1035, 1292
BstMAI GTCTC 2 cut(s) 316, 788
BstMBI GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
BstMWI GCNNNNNNNGC 2 cut(s) 225, 978
BstNI CCWGG 2 cut(s) 1051, 1507
BstSCI CCNGG 3 cut(s) 499, 1049, 1505
BstSFI CTRYAG 1 cut(s) 1128
BstV1I GCAGC 5 cut(s) 231, 468, 1142, 1157, 1430
BstV2I GAAGAC 1 cut(s) 1193
BsuI GTATCC 2 cut(s) 1384, 1589
BsuRI GGCC 4 cut(s) 498, 635, 720, 770
BtgI CCRYGG 3 cut(s) 16, 1386, 1527
BtrI CACGTC 2 cut(s) 1064, 1401
BtsCI GGATG 4 cut(s) 222, 290, 842, 1083
BtsI GCAGTG 3 cut(s) 76, 979, 988
BtsIMutI CAGTG 9 cut(s) 76, 214, 685, 715, 914, 979, 988, 1195, 1316
Cac8I GCNNGC 1 cut(s) 1006
CfoI GCGC 1 cut(s) 991
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 3 cut(s) 497, 1016, 1560
CseI GACGC 1 cut(s) 875
Csp6I GTAC 6 cut(s) 13, 1107, 1236, 1452, 1542, 1577
CspAI ACCGGT 1 cut(s) 650
CspCI CAANNNNNGTGG 4 cut(s) 1045, 1080, 1329, 1364
CviAII CATG 5 cut(s) 368, 426, 1141, 1346, 1528
CviQI GTAC 6 cut(s) 13, 1107, 1236, 1452, 1542, 1577
DpnI GATC 6 cut(s) 60, 183, 282, 627, 1034, 1291
DpnII GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 1527
Eco24I GRGCYC 1 cut(s) 190
Eco32I GATATC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 1016, 1560
Eco57I CTGAAG 2 cut(s) 222, 1209
EcoO109I RGGNCCY 1 cut(s) 1560
EcoRII CCWGG 2 cut(s) 1049, 1505
EcoRV GATATC 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 1527
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 1527
FaeI CATG 5 cut(s) 371, 429, 1144, 1349, 1531
FalI AAGNNNNNCTT 4 cut(s) 875, 907, 1158, 1190
FaqI GGGAC 3 cut(s) 34, 1318, 1573
FatI CATG 5 cut(s) 367, 425, 1140, 1345, 1527
FauNDI CATATG 1 cut(s) 1522
FblI GTMKAC 1 cut(s) 1154
Fnu4HI GCNGC 6 cut(s) 220, 482, 768, 1131, 1146, 1444
FokI GGATG 4 cut(s) 209, 277, 849, 1090
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 6 cut(s) 220, 482, 768, 1131, 1146, 1444
FspBI CTAG 2 cut(s) 849, 1022
GlaI GCGC 1 cut(s) 990
GluI GCNGC 6 cut(s) 220, 482, 768, 1131, 1146, 1444
GsaI CCCAGC 2 cut(s) 743, 1442
GsuI CTGGAG 2 cut(s) 886, 1528
HaeIII GGCC 4 cut(s) 498, 635, 720, 770
HapII CCGG 3 cut(s) 284, 501, 651
HgaI GACGC 1 cut(s) 875
HhaI GCGC 1 cut(s) 991
Hin1II CATG 5 cut(s) 371, 429, 1144, 1349, 1531
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HincII GTYRAC 2 cut(s) 468, 874
HindII GTYRAC 2 cut(s) 468, 874
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 3 cut(s) 29, 433, 1182
HpaI GTTAAC 1 cut(s) 468
HpaII CCGG 3 cut(s) 284, 501, 651
HphI GGTGA 2 cut(s) 306, 1268
Hpy166II GTNNAC 7 cut(s) 468, 874, 1155, 1195, 1238, 1251, 1276
Hpy188I TCNGA 5 cut(s) 34, 41, 163, 241, 625
Hpy188III TCNNGA 4 cut(s) 278, 574, 821, 834
Hpy8I GTNNAC 7 cut(s) 468, 874, 1155, 1195, 1238, 1251, 1276
HpyAV CCTTC 7 cut(s) 132, 246, 557, 561, 710, 1236, 1474
HpyCH4III ACNGT 6 cut(s) 17, 209, 1159, 1199, 1311, 1456
HpyCH4IV ACGT 2 cut(s) 1063, 1400
HpyCH4V TGCA 8 cut(s) 256, 299, 356, 484, 1008, 1130, 1415, 1484
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 978
HpySE526I ACGT 2 cut(s) 1063, 1400
Hsp92II CATG 5 cut(s) 371, 429, 1144, 1349, 1531
HspAI GCGC 1 cut(s) 989
KflI GGGWCCC 1 cut(s) 1560
KpnI GGTACC 1 cut(s) 16
KspAI GTTAAC 1 cut(s) 468
Kzo9I GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
LguI GCTCTTC 1 cut(s) 195
Lsp1109I GCAGC 5 cut(s) 231, 468, 1142, 1157, 1430
LweI GCATC 3 cut(s) 231, 343, 909
MaeI CTAG 2 cut(s) 849, 1022
MaeII ACGT 2 cut(s) 1063, 1400
MaeIII GTNAC 7 cut(s) 286, 514, 653, 887, 974, 1221, 1531
MalI GATC 6 cut(s) 60, 183, 282, 627, 1034, 1291
MboI GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
MboII GAAGA 3 cut(s) 182, 1174, 1193
MhlI GDGCHC 2 cut(s) 118, 190
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 5 cut(s) 407, 924, 1113, 1213, 1427
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 2 cut(s) 23, 427
MmeI TCCRAC 2 cut(s) 393, 994
Mox20I TGGCCA 1 cut(s) 635
MscI TGGCCA 1 cut(s) 635
MseI TTAA 3 cut(s) 86, 467, 749
Msp20I TGGCCA 1 cut(s) 635
MspI CCGG 3 cut(s) 284, 501, 651
MspR9I CCNGG 3 cut(s) 501, 1051, 1507
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 2 cut(s) 1051, 1507
MwoI GCNNNNNNNGC 2 cut(s) 225, 978
NciI CCSGG 1 cut(s) 501
NcoI CCATGG 1 cut(s) 1527
NdeI CATATG 1 cut(s) 1522
NdeII GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
NlaIII CATG 5 cut(s) 371, 429, 1144, 1349, 1531
NlaIV GGNNCC 5 cut(s) 14, 499, 689, 1561, 1562
NmuCI GTSAC 2 cut(s) 887, 974
PciSI GCTCTTC 1 cut(s) 195
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 1182
PinAI ACCGGT 1 cut(s) 650
PkrI GCNGC 6 cut(s) 221, 483, 769, 1132, 1147, 1445
PleI GAGTC 2 cut(s) 23, 427
PpsI GAGTC 2 cut(s) 23, 427
PpuMI RGGWCCY 1 cut(s) 1560
Psp5II RGGWCCY 1 cut(s) 1560
Psp6I CCWGG 2 cut(s) 1049, 1505
PspFI CCCAGC 2 cut(s) 739, 1438
PspGI CCWGG 2 cut(s) 1049, 1505
PspN4I GGNNCC 5 cut(s) 14, 499, 689, 1561, 1562
PspPI GGNCC 3 cut(s) 497, 1016, 1560
PspPPI RGGWCCY 1 cut(s) 1560
PstI CTGCAG 1 cut(s) 1132
RsaI GTAC 6 cut(s) 14, 1108, 1237, 1453, 1543, 1578
RsaNI GTAC 6 cut(s) 13, 1107, 1236, 1452, 1542, 1577
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 86, 467, 749
SatI GCNGC 6 cut(s) 220, 482, 768, 1131, 1146, 1444
Sau3AI GATC 6 cut(s) 58, 181, 280, 625, 1032, 1289
Sau96I GGNCC 3 cut(s) 497, 1016, 1560
SchI GAGTC 2 cut(s) 23, 427
ScrFI CCNGG 3 cut(s) 501, 1051, 1507
SduI GDGCHC 2 cut(s) 118, 190
SfaNI GCATC 3 cut(s) 231, 343, 909
SfcI CTRYAG 1 cut(s) 1128
SinI GGWCC 2 cut(s) 1016, 1560
Sse9I AATT 5 cut(s) 407, 924, 1113, 1213, 1427
SsiI CCGC 2 cut(s) 226, 767
SspMI CTAG 2 cut(s) 849, 1022
StyD4I CCNGG 3 cut(s) 499, 1049, 1505
StyI CCWWGG 1 cut(s) 1527
TaaI ACNGT 6 cut(s) 17, 209, 1159, 1199, 1311, 1456
TaiI ACGT 2 cut(s) 1066, 1403
TaqI TCGA 2 cut(s) 61, 543
TasI AATT 5 cut(s) 407, 924, 1113, 1213, 1427
TatI WGTACW 1 cut(s) 1235
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 1 cut(s) 1182
Tru1I TTAA 3 cut(s) 86, 467, 749
Tru9I TTAA 3 cut(s) 86, 467, 749
TscAI CASTG 9 cut(s) 76, 214, 685, 715, 921, 979, 988, 1202, 1316
TseFI GTSAC 2 cut(s) 887, 974
TseI GCWGC 5 cut(s) 219, 481, 1130, 1145, 1443
Tsp45I GTSAC 2 cut(s) 887, 974
TspDTI ATGAA 4 cut(s) 72, 414, 603, 1456
TspGWI ACGGA 1 cut(s) 1375
TspRI CASTG 9 cut(s) 76, 214, 685, 715, 921, 979, 988, 1202, 1316
VpaK11BI GGWCC 2 cut(s) 1016, 1560
XapI RAATTY 1 cut(s) 1213
XmiI GTMKAC 1 cut(s) 1154
XspI CTAG 2 cut(s) 849, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.