FvH4_2g29410

At2g34160-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002909
Physical Location & Seq
Reverse (-)
22817942 .. 22820051
2110 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29410.t1

Sequence Viewer

Length: 405 bp
ATGGAGGGTCTGACGGAGGCAGTGAACGACATGCACGTCACGGAGTCGGCGGCGAACAAGAAGAATCATCGCCTCCAGAATCGCATCCAAGTCTCCAACACCAAGAAACCCCTCTTCTTCTATGTCAATCTCGCCAAGAAGTACATGCAGCTCAACAATGAGGTCGAGCTTTCCGGTCTCGGAATGGCTATTGCTACAGTTGTCACCATTGCTGAGATTCTCAAGAACAATGGATTGGCTGTCGAGAAGAAGATCATCACCAAAACTGTTGACATGAGGGAGGATGCAGGAGGGCGCCCAATTCAGAAAGCAAAGATTGAAATACTGCTTGGAAAGTCAGACAAGTTTGATGAGATACTGGCTGCTGCAGCTGAGGAAGCCAATGAATATGAGGACCAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

135

Amino Acids

14.88

Weight (kDa)

5.75

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 27 - 87 3.2e-13 Alba
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 294
AciI CCGC 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 295
AfaI GTAC 1 cut(s) 143
AgsI TTSAA 1 cut(s) 320
AjiI CACGTC 1 cut(s) 37
AjuI GAANNNNNNNTTGG 4 cut(s) 218, 250, 312, 344
AluBI AGCT 3 cut(s) 151, 169, 371
AluI AGCT 3 cut(s) 151, 169, 371
Alw26I GTCTC 2 cut(s) 97, 182
ApeKI GCWGC 4 cut(s) 148, 362, 365, 368
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 2 cut(s) 196, 250
AvaII GGWCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 294
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 4 cut(s) 160, 349, 352, 380
BcoDI GTCTC 2 cut(s) 97, 182
BfmI CTRYAG 2 cut(s) 195, 366
BfoI RGCGCY 1 cut(s) 298
BisI GCNGC 5 cut(s) 51, 149, 363, 366, 369
BlsI GCNGC 5 cut(s) 52, 150, 364, 367, 370
Bme18I GGWCC 1 cut(s) 394
BmgBI CACGTC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 1 cut(s) 296
BmsI GCATC 2 cut(s) 93, 274
BpmI CTGGAG 1 cut(s) 59
Bpu10I CCTNAGC 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 295
BsaI GGTCTC 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 363
Bse3DI GCAATG 1 cut(s) 207
BseGI GGATG 2 cut(s) 84, 289
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 204, 363
BseNI ACTGG 1 cut(s) 363
BseXI GCAGC 4 cut(s) 160, 349, 352, 380
BshNI GGYRCC 1 cut(s) 294
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 2 cut(s) 97, 182
Bso31I GGTCTC 1 cut(s) 182
Bsp143I GATC 1 cut(s) 252
BspACI CCGC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 205, 364
BspLI GGNNCC 1 cut(s) 296
BspMAI CTGCAG 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 294
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 207
BsrI ACTGG 1 cut(s) 363
BssMI GATC 1 cut(s) 252
BssNI GRCGYC 1 cut(s) 295
Bst4CI ACNGT 2 cut(s) 199, 268
Bst6I CTCTTC 1 cut(s) 119
BstACI GRCGYC 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 213, 372
BstF5I GGATG 2 cut(s) 84, 289
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 1 cut(s) 255
BstMAI GTCTC 2 cut(s) 97, 182
BstMBI GATC 1 cut(s) 252
BstMWI GCNNNNNNNGC 2 cut(s) 368, 377
BstNSI RCATGY 2 cut(s) 34, 148
BstSFI CTRYAG 2 cut(s) 195, 366
BstV1I GCAGC 4 cut(s) 160, 349, 352, 380
BtgZI GCGATG 1 cut(s) 53
BtrI CACGTC 1 cut(s) 37
BtsCI GGATG 2 cut(s) 84, 289
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 27
CfoI GCGC 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 394
Csp6I GTAC 1 cut(s) 142
CviAII CATG 3 cut(s) 31, 145, 274
CviJI RGCY 7 cut(s) 151, 169, 188, 239, 362, 371, 380
CviKI_1 RGCY 7 cut(s) 151, 169, 188, 239, 362, 371, 380
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 213, 372
DinI GGCGCC 1 cut(s) 296
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 394
EgeI GGCGCC 1 cut(s) 296
EheI GGCGCC 1 cut(s) 296
FaeI CATG 3 cut(s) 34, 148, 277
FaiI YATR 5 cut(s) 32, 123, 146, 275, 390
FatI CATG 3 cut(s) 30, 144, 273
Fnu4HI GCNGC 5 cut(s) 51, 149, 363, 366, 369
FokI GGATG 2 cut(s) 71, 296
Fsp4HI GCNGC 5 cut(s) 51, 149, 363, 366, 369
GlaI GCGC 1 cut(s) 296
GluI GCNGC 5 cut(s) 51, 149, 363, 366, 369
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 298
HapII CCGG 1 cut(s) 174
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 3 cut(s) 34, 148, 277
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 4 cut(s) 44, 64, 79, 217
HpaII CCGG 1 cut(s) 174
HphI GGTGA 2 cut(s) 196, 250
Hpy166II GTNNAC 2 cut(s) 25, 271
Hpy188I TCNGA 4 cut(s) 12, 182, 306, 340
Hpy188III TCNNGA 3 cut(s) 76, 223, 244
Hpy8I GTNNAC 2 cut(s) 25, 271
HpyCH4III ACNGT 2 cut(s) 199, 268
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 4 cut(s) 34, 148, 287, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 377
HpyF3I CTNAG 2 cut(s) 213, 372
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 3 cut(s) 34, 148, 277
HspAI GCGC 1 cut(s) 295
KasI GGCGCC 1 cut(s) 294
Kzo9I GATC 1 cut(s) 252
LpnPI CCDG 4 cut(s) 89, 187, 273, 344
Lsp1109I GCAGC 4 cut(s) 160, 349, 352, 380
LweI GCATC 2 cut(s) 93, 274
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 37, 202
MalI GATC 1 cut(s) 254
MboI GATC 1 cut(s) 252
MboII GAAGA 5 cut(s) 73, 106, 109, 259, 262
MluCI AATT 1 cut(s) 300
Mly113I GGCGCC 1 cut(s) 295
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 9 cut(s) 10, 83, 122, 154, 270, 274, 284, 367, 385
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 368, 377
NarI GGCGCC 1 cut(s) 295
NdeII GATC 1 cut(s) 252
NlaIII CATG 3 cut(s) 34, 148, 277
NlaIV GGNNCC 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 37, 202
NspI RCATGY 2 cut(s) 34, 148
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 3 cut(s) 64, 79, 217
PkrI GCNGC 5 cut(s) 52, 150, 364, 367, 370
PleI GAGTC 1 cut(s) 52
PluTI GGCGCC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 1 cut(s) 394
PstI CTGCAG 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SatI GCNGC 5 cut(s) 51, 149, 363, 366, 369
Sau3AI GATC 1 cut(s) 252
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 1 cut(s) 53
SetI ASST 5 cut(s) 39, 153, 165, 171, 373
SfaNI GCATC 2 cut(s) 93, 274
SfcI CTRYAG 2 cut(s) 195, 366
SfoI GGCGCC 1 cut(s) 296
SinI GGWCC 1 cut(s) 394
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 1 cut(s) 300
SsiI CCGC 1 cut(s) 50
SspDI GGCGCC 1 cut(s) 294
TaaI ACNGT 2 cut(s) 199, 268
TaiI ACGT 1 cut(s) 39
TaqI TCGA 2 cut(s) 165, 243
TasI AATT 1 cut(s) 300
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 3 cut(s) 64, 79, 217
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 2 cut(s) 37, 202
TseI GCWGC 4 cut(s) 148, 362, 365, 368
Tsp45I GTSAC 2 cut(s) 37, 202
TspDTI ATGAA 1 cut(s) 399
TspGWI ACGGA 2 cut(s) 29, 56
TspRI CASTG 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 394
XceI RCATGY 2 cut(s) 34, 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.