MD17G1207900.v1.1

At2g34160-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002909
Physical Location & Seq
Forward (+)
25079410 .. 25082228
2819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1207900.v1.1.491

Sequence Viewer

Length: 576 bp
ATGCTTGCATCCTTATCTCTTCGGACCGTGTACAGTTGGGTCCATCTAGGCCCATATATAAGAGAAACCCAAACAAACCTTACAGAGAAAACAATTGTAGAAAGAGAAGCCAGAGAGAGAGAGAGTAGTCAGAAGGGGGATTTAGGGTTAGGGTTTTTTGAAGCTGTTCACTCAGTTGCAATGGAGGAGATCACTGAAGGCGTGAACAATATCACCGTCAATGCCTCCGATTCGCACAAGAAGAACCGAATTCAGGTATCGAATACCAAGAAGCCACTCTTCTTCTATGTAAATCTTGCCAAGAGGTATATGCAACAGTACAATGAGGTGGAGCTCTCTGCTTTGGGAATGGCTATTGCCACAGTTGTCACAATCGCAGAAATTCTGAAAAACAATGGACTGGCTGTTGAGAAAAAAATCATGACATCGACTGTTGACATTAAGGATGATTCTAGGGGAAGACCAGTGCAAAAGGCCAAGATTGAGATAGTACTTGGGAAGTCGGAAGACTTTGATGACTTGATGGCCGCTGCAGCTGAGGAGAGGTTGATTGCAGCTGCAGAGGAGCAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

192

Amino Acids

21.31

Weight (kDa)

5.53

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 82 - 143 3e-13 Alba
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 528
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 249, 381
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 3 cut(s) 32, 320, 492
AfiI CCNNNNNNNGG 1 cut(s) 253
AgsI TTSAA 1 cut(s) 161
AluBI AGCT 5 cut(s) 164, 334, 536, 557, 573
AluI AGCT 5 cut(s) 164, 334, 536, 557, 573
Alw21I GWGCWC 1 cut(s) 336
AoxI GGCC 3 cut(s) 49, 474, 525
ApeKI GCWGC 4 cut(s) 530, 533, 554, 557
ApoI RAATTY 2 cut(s) 249, 381
Asp700I GAANNNNTTC 1 cut(s) 165
AspS9I GGNCC 3 cut(s) 24, 40, 50
AsuHPI GGTGA 1 cut(s) 205
AvaII GGWCC 2 cut(s) 24, 40
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 2 cut(s) 466, 513
Bbv12I GWGCWC 1 cut(s) 336
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 4 cut(s) 517, 544, 545, 566
BccI CCATC 2 cut(s) 51, 517
BfaI CTAG 2 cut(s) 47, 453
BfmI CTRYAG 2 cut(s) 531, 558
BisI GCNGC 5 cut(s) 528, 531, 534, 555, 558
BlsI GCNGC 5 cut(s) 529, 532, 535, 556, 559
BmcAI AGTACT 1 cut(s) 492
Bme18I GGWCC 2 cut(s) 24, 40
BmgT120I GGNCC 3 cut(s) 24, 40, 50
BmiI GGNNCC 1 cut(s) 41
BmsI GCATC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 466, 513
Bpu10I CCTNAGC 1 cut(s) 537
Bsc4I CCNNNNNNNGG 1 cut(s) 253
Bse1I ACTGG 2 cut(s) 405, 464
Bse3DI GCAATG 1 cut(s) 186
BseGI GGATG 2 cut(s) 8, 451
BseLI CCNNNNNNNGG 1 cut(s) 253
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 186, 528
BseNI ACTGG 2 cut(s) 405, 464
BseRI GAGGAG 2 cut(s) 200, 554
BseXI GCAGC 4 cut(s) 517, 544, 545, 566
BshFI GGCC 3 cut(s) 51, 476, 527
BsiHKAI GWGCWC 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 253
BsnI GGCC 3 cut(s) 51, 476, 527
Bsp1286I GDGCHC 1 cut(s) 336
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 1 cut(s) 528
BspANI GGCC 3 cut(s) 51, 476, 527
BspCNI CTCAG 2 cut(s) 185, 529
BspHI TCATGA 1 cut(s) 420
BspLI GGNNCC 1 cut(s) 41
BspMAI CTGCAG 2 cut(s) 535, 562
BsrDI GCAATG 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 2 cut(s) 405, 464
BssMI GATC 1 cut(s) 189
Bst4CI ACNGT 6 cut(s) 28, 35, 217, 318, 364, 433
Bst6I CTCTTC 2 cut(s) 24, 284
BstAUI TGTACA 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 172, 537
BstF5I GGATG 2 cut(s) 8, 451
BstKTI GATC 1 cut(s) 192
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstSFI CTRYAG 2 cut(s) 531, 558
BstV1I GCAGC 4 cut(s) 517, 544, 545, 566
BstV2I GAAGAC 2 cut(s) 466, 513
BsuRI GGCC 3 cut(s) 51, 476, 527
BtsCI GGATG 2 cut(s) 8, 451
BtsIMutI CAGTG 2 cut(s) 192, 471
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 1 cut(s) 420
Cfr13I GGNCC 3 cut(s) 24, 40, 50
CpoI CGGWCCG 1 cut(s) 24
Csp6I GTAC 3 cut(s) 31, 319, 491
CspI CGGWCCG 1 cut(s) 24
CviAII CATG 1 cut(s) 421
CviQI GTAC 3 cut(s) 31, 319, 491
DdeI CTNAG 2 cut(s) 172, 537
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 24, 284
EarI CTCTTC 2 cut(s) 24, 284
Ecl136II GAGCTC 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 24, 40
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 334
EcoRI GAATTC 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 336
FaeI CATG 1 cut(s) 424
FaiI YATR 7 cut(s) 55, 57, 59, 288, 309, 311, 422
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 1 cut(s) 420
Fnu4HI GCNGC 5 cut(s) 528, 531, 534, 555, 558
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 5 cut(s) 528, 531, 534, 555, 558
FspBI CTAG 2 cut(s) 47, 453
GluI GCNGC 5 cut(s) 528, 531, 534, 555, 558
HaeIII GGCC 3 cut(s) 51, 476, 527
Hin1II CATG 1 cut(s) 424
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 2 cut(s) 230, 449
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 4 cut(s) 31, 169, 205, 436
Hpy188I TCNGA 5 cut(s) 24, 132, 229, 387, 505
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 4 cut(s) 31, 169, 205, 436
HpyAV CCTTC 2 cut(s) 127, 191
HpyCH4III ACNGT 6 cut(s) 28, 35, 217, 318, 364, 433
HpyCH4V TGCA 7 cut(s) 8, 179, 313, 469, 533, 554, 560
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 2 cut(s) 172, 537
Hsp92II CATG 1 cut(s) 424
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 2 cut(s) 331, 565
LpnPI CCDG 4 cut(s) 124, 239, 386, 477
Lsp1109I GCAGC 4 cut(s) 517, 544, 545, 566
LweI GCATC 1 cut(s) 17
MaeI CTAG 2 cut(s) 47, 453
MaeIII GTNAC 1 cut(s) 367
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 6 cut(s) 11, 253, 271, 274, 471, 518
MfeI CAATTG 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 3 cut(s) 93, 249, 381
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 7 cut(s) 178, 235, 297, 319, 532, 537, 556
MroXI GAANNNNTTC 1 cut(s) 165
MseI TTAA 1 cut(s) 441
MspA1I CMGCKG 3 cut(s) 530, 536, 557
MunI CAATTG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 533
NdeII GATC 1 cut(s) 189
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 367
PagI TCATGA 1 cut(s) 420
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 2 cut(s) 230, 449
PkrI GCNGC 5 cut(s) 529, 532, 535, 556, 559
Psp124BI GAGCTC 1 cut(s) 336
PspN4I GGNNCC 1 cut(s) 41
PspPI GGNCC 3 cut(s) 24, 40, 50
PstI CTGCAG 2 cut(s) 535, 562
PvuII CAGCTG 2 cut(s) 536, 557
RsaI GTAC 3 cut(s) 32, 320, 492
RsaNI GTAC 3 cut(s) 31, 319, 491
Rsr2I CGGWCCG 1 cut(s) 24
RsrII CGGWCCG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 5 cut(s) 528, 531, 534, 555, 558
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 3 cut(s) 24, 40, 50
ScaI AGTACT 1 cut(s) 492
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 1 cut(s) 17
SfcI CTRYAG 2 cut(s) 531, 558
SinI GGWCC 2 cut(s) 24, 40
Sse9I AATT 3 cut(s) 93, 249, 381
SsiI CCGC 1 cut(s) 528
SspMI CTAG 2 cut(s) 47, 453
SstI GAGCTC 1 cut(s) 336
TaaI ACNGT 6 cut(s) 28, 35, 217, 318, 364, 433
TaqI TCGA 2 cut(s) 260, 428
TasI AATT 3 cut(s) 93, 249, 381
TatI WGTACW 3 cut(s) 30, 318, 490
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 2 cut(s) 230, 449
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 2 cut(s) 199, 471
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 4 cut(s) 530, 533, 554, 557
Tsp45I GTSAC 1 cut(s) 367
TspRI CASTG 2 cut(s) 199, 471
VpaK11BI GGWCC 2 cut(s) 24, 40
XapI RAATTY 2 cut(s) 249, 381
XmnI GAANNNNTTC 1 cut(s) 165
XspI CTAG 2 cut(s) 47, 453
ZrmI AGTACT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.