MD16G1209300.v1.1

Alba

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002909
Physical Location & Seq
Forward (+)
19789313 .. 19790988
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1209300.v1.1.491

Sequence Viewer

Length: 483 bp
ATGAAGGCTTCGGTGACAGTGAACGACAACAAGCCTGCTGTCGTTGCTACTGGCGGTGGTGCCTGCGGTGTTGGGGAGGCCATTGCTACATGCGGTGTTGGCGAGGCCATTGCTACATGCGAAGGTGGGGATGTTGCTACCGCTGAGACTCAGAAGACGAAGAAGCTGAAGAACAAGAATCGGATTCACATTTCGCACACCAAGCGTCCATTCTTCTTCTACTGCAATCTTGCCAAGAGGTACATCAAGCAGTACAATGCAGTTGAGCTCTCTGCATTGGGAATGGCTATTCCTACTCTGATCACAATTGCTGAGAGTCTCAAGAGCAATGGAGTTGCTGTCGAGAAGAGCATAAGCACCTCTACTGTTGTCTCGAAGCTGGATAGCGAAGATGGACGGATTGTGTTTAAGGCTCAGTTGGCGATTTTGCTGGGAAAGGCTGAAGAACTCGAGGAGACCGCAGTTCCTGTGGCTGCAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

161

Amino Acids

16.71

Weight (kDa)

8.66

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 60 - 115 4.5e-08 Alba
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AciI CCGC 5 cut(s) 54, 66, 93, 141, 459
AcuI CTGAAG 2 cut(s) 188, 462
AfaI GTAC 2 cut(s) 242, 254
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 3 cut(s) 166, 268, 379
AluI AGCT 3 cut(s) 166, 268, 379
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 4 cut(s) 140, 323, 376, 449
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 2 cut(s) 78, 105
ApeKI GCWGC 2 cut(s) 473, 476
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 449
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 460
BccI CCATC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 4 cut(s) 140, 323, 376, 449
BfmI CTRYAG 1 cut(s) 474
BisI GCNGC 2 cut(s) 474, 477
BlsI GCNGC 2 cut(s) 475, 478
BmeT110I CYCGRG 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 61
BpiI GAAGAC 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 449
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse1I ACTGG 1 cut(s) 55
Bse3DI GCAATG 3 cut(s) 81, 108, 334
BseGI GGATG 1 cut(s) 136
BseMI GCAATG 3 cut(s) 81, 108, 334
BseMII CTCAG 4 cut(s) 135, 164, 303, 428
BseNI ACTGG 1 cut(s) 55
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 1 cut(s) 460
BseYI CCCAGC 1 cut(s) 430
BshFI GGCC 2 cut(s) 80, 107
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 270
BsiHKCI CYCGRG 1 cut(s) 449
BsmAI GTCTC 4 cut(s) 140, 323, 376, 449
BsnI GGCC 2 cut(s) 80, 107
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 1 cut(s) 300
BspACI CCGC 5 cut(s) 54, 66, 93, 141, 459
BspANI GGCC 2 cut(s) 80, 107
BspCNI CTCAG 4 cut(s) 136, 163, 304, 427
BspLI GGNNCC 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 478
BspQI GCTCTTC 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 3 cut(s) 81, 108, 334
BsrI ACTGG 1 cut(s) 55
BssMI GATC 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 19, 367
Bst6I CTCTTC 1 cut(s) 341
BstC8I GCNNGC 2 cut(s) 36, 64
BstDEI CTNAG 4 cut(s) 144, 150, 312, 414
BstF5I GGATG 1 cut(s) 136
BstKTI GATC 1 cut(s) 303
BstMAI GTCTC 4 cut(s) 140, 323, 376, 449
BstMBI GATC 1 cut(s) 300
BstMWI GCNNNNNNNGC 4 cut(s) 44, 99, 202, 419
BstNSI RCATGY 2 cut(s) 93, 120
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 460
BstV2I GAAGAC 1 cut(s) 161
BsuRI GGCC 2 cut(s) 80, 107
BtsCI GGATG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 36, 64
CaiI CAGNNNCTG 1 cut(s) 467
CseI GACGC 1 cut(s) 194
Csp6I GTAC 2 cut(s) 241, 253
CviAII CATG 2 cut(s) 90, 117
CviQI GTAC 2 cut(s) 241, 253
DdeI CTNAG 4 cut(s) 144, 150, 312, 414
DpnI GATC 1 cut(s) 302
DpnII GATC 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 188, 462
Eco88I CYCGRG 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 2 cut(s) 93, 120
FaiI YATR 3 cut(s) 91, 118, 353
FatI CATG 2 cut(s) 89, 116
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 474, 477
FokI GGATG 1 cut(s) 143
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 2 cut(s) 474, 477
GluI GCNGC 2 cut(s) 474, 477
GsaI CCCAGC 1 cut(s) 434
HaeIII GGCC 2 cut(s) 80, 107
HgaI GACGC 1 cut(s) 194
Hin1II CATG 2 cut(s) 93, 120
HinfI GANTC 4 cut(s) 148, 178, 184, 316
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 3 cut(s) 153, 183, 300
Hpy188III TCNNGA 3 cut(s) 322, 343, 373
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 1 cut(s) 116
HpyCH4III ACNGT 2 cut(s) 19, 367
HpyCH4V TGCA 4 cut(s) 225, 260, 275, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 99, 202, 419
HpyF3I CTNAG 4 cut(s) 144, 150, 312, 414
Hsp92II CATG 2 cut(s) 93, 120
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 1 cut(s) 300
LguI GCTCTTC 1 cut(s) 341
LpnPI CCDG 5 cut(s) 36, 48, 76, 365, 416
Lsp1109I GCAGC 1 cut(s) 460
MaeIII GTNAC 1 cut(s) 13
MalI GATC 1 cut(s) 302
MboI GATC 1 cut(s) 300
MboII GAAGA 8 cut(s) 166, 172, 181, 205, 208, 358, 401, 455
MfeI CAATTG 1 cut(s) 306
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 1 cut(s) 306
MlyI GAGTC 2 cut(s) 142, 325
MnlI CCTC 5 cut(s) 70, 97, 231, 370, 445
MseI TTAA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 143
MunI CAATTG 1 cut(s) 306
MwoI GCNNNNNNNGC 4 cut(s) 44, 99, 202, 419
NdeII GATC 1 cut(s) 300
NlaIII CATG 2 cut(s) 93, 120
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 2 cut(s) 93, 120
PaeR7I CTCGAG 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 341
PfeI GAWTC 2 cut(s) 178, 184
PkrI GCNGC 2 cut(s) 475, 478
PleI GAGTC 2 cut(s) 142, 324
PpsI GAGTC 2 cut(s) 142, 324
Psp124BI GAGCTC 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 430
PspN4I GGNNCC 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 449
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 467
RsaI GTAC 2 cut(s) 242, 254
RsaNI GTAC 2 cut(s) 241, 253
SacI GAGCTC 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 2 cut(s) 474, 477
Sau3AI GATC 1 cut(s) 300
SchI GAGTC 2 cut(s) 142, 325
SduI GDGCHC 1 cut(s) 270
SetI ASST 6 cut(s) 127, 168, 242, 270, 362, 381
SfcI CTRYAG 1 cut(s) 474
Sfr274I CTCGAG 1 cut(s) 449
SlaI CTCGAG 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 320, 449
SmoI CTYRAG 2 cut(s) 320, 449
Sse9I AATT 1 cut(s) 306
SsiI CCGC 5 cut(s) 54, 66, 93, 141, 459
SstI GAGCTC 1 cut(s) 270
TaaI ACNGT 2 cut(s) 19, 367
TaqI TCGA 3 cut(s) 342, 374, 450
TasI AATT 1 cut(s) 306
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 2 cut(s) 178, 184
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 2 cut(s) 473, 476
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 1 cut(s) 24
XceI RCATGY 2 cut(s) 93, 120
XhoI CTCGAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.