FvH4_6g30150

At2g34160-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002909
Physical Location & Seq
Reverse (-)
23293178 .. 23296318
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30150.t1

Sequence Viewer

Length: 411 bp
ATGGAGGCCGTTGGAGAGATTACCGAGGCTATGAACAACGTCAACATCAACAACTCCGATTCGCACAAGAAGAACCGTATTCAGGTCTCAAACACCAAGAAGCCCCTCTTCTTCTACGTCAATCTCGCCAAGAGGTATATGCAGCAGTACAATGAGGTGGAGCTATCTGCCTTGGGAATGGCTATTGCCACAGTTGTCACAATTGCAGAAATTCTGAAAAACAATGGGCTGGCTGTTGAGAAAAAAATCATGACATCAACTGTTGACATAAAGGATGATTCTAGAGGGAGACCAGTCCAAAAAGCCAAGATTGAGATATTGCTTGGGAAGACAGCAAATTTCGATGACTTGATGGCCGCGGCTGCTGAGGAGAGGGAACTTGCAGCAGCAGAGGCTGAGGAGCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

137

Amino Acids

15.03

Weight (kDa)

5.27

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 25 - 86 1.4e-13 Alba
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 359
AciI CCGC 2 cut(s) 357, 359
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 210, 337
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 82
AluBI AGCT 2 cut(s) 163, 408
AluI AGCT 2 cut(s) 163, 408
Alw26I GTCTC 2 cut(s) 91, 283
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 2 cut(s) 6, 354
ApeKI GCWGC 4 cut(s) 142, 362, 383, 386
ApoI RAATTY 2 cut(s) 210, 337
BbsI GAAGAC 1 cut(s) 335
BbvCI CCTCAGC 2 cut(s) 366, 396
BbvI GCAGC 4 cut(s) 154, 349, 395, 398
BccI CCATC 1 cut(s) 346
BcoDI GTCTC 2 cut(s) 91, 283
BfaI CTAG 1 cut(s) 282
BisI GCNGC 6 cut(s) 143, 357, 360, 363, 384, 387
BlsI GCNGC 6 cut(s) 144, 358, 361, 364, 385, 388
BpiI GAAGAC 1 cut(s) 335
Bpu10I CCTNAGC 2 cut(s) 366, 396
BsaI GGTCTC 2 cut(s) 91, 283
BsaJI CCNNGG 3 cut(s) 24, 171, 357
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 293
BseDI CCNNGG 3 cut(s) 24, 171, 357
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMII CTCAG 2 cut(s) 357, 387
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 4 cut(s) 154, 349, 395, 398
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 8, 356
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 2 cut(s) 91, 283
BsnI GGCC 2 cut(s) 8, 356
Bso31I GGTCTC 2 cut(s) 91, 283
BspACI CCGC 2 cut(s) 357, 359
BspANI GGCC 2 cut(s) 8, 356
BspCNI CTCAG 2 cut(s) 358, 388
BspFNI CGCG 1 cut(s) 359
BspHI TCATGA 1 cut(s) 249
BspTNI GGTCTC 2 cut(s) 91, 283
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 3 cut(s) 24, 171, 357
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 77, 193, 262
Bst6I CTCTTC 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 366, 396
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 359
BstMAI GTCTC 2 cut(s) 91, 283
BstMWI GCNNNNNNNGC 2 cut(s) 362, 392
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 4 cut(s) 154, 349, 395, 398
BstV2I GAAGAC 1 cut(s) 335
BsuRI GGCC 2 cut(s) 8, 356
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 1 cut(s) 280
Cac8I GCNNGC 1 cut(s) 231
CaiI CAGNNNCTG 1 cut(s) 395
CciI TCATGA 1 cut(s) 249
Cfr42I CCGCGG 1 cut(s) 360
Csp6I GTAC 1 cut(s) 148
CviAII CATG 1 cut(s) 250
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 2 cut(s) 366, 396
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 91, 283
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 1 cut(s) 253
FaiI YATR 5 cut(s) 32, 138, 140, 251, 269
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FatI CATG 1 cut(s) 249
Fnu4HI GCNGC 6 cut(s) 143, 357, 360, 363, 384, 387
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 6 cut(s) 143, 357, 360, 363, 384, 387
FspBI CTAG 1 cut(s) 282
GluI GCNGC 6 cut(s) 143, 357, 360, 363, 384, 387
HaeIII GGCC 2 cut(s) 8, 356
Hin1II CATG 1 cut(s) 253
HincII GTYRAC 2 cut(s) 43, 265
HindII GTYRAC 2 cut(s) 43, 265
HinfI GANTC 2 cut(s) 59, 278
Hpy166II GTNNAC 2 cut(s) 43, 265
Hpy188I TCNGA 2 cut(s) 58, 216
Hpy188III TCNNGA 2 cut(s) 250, 282
Hpy8I GTNNAC 2 cut(s) 43, 265
HpyCH4III ACNGT 3 cut(s) 77, 193, 262
HpyCH4IV ACGT 2 cut(s) 39, 117
HpyCH4V TGCA 3 cut(s) 142, 206, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 392
HpyF3I CTNAG 2 cut(s) 366, 396
HpySE526I ACGT 2 cut(s) 39, 117
Hsp92II CATG 1 cut(s) 253
KspI CCGCGG 1 cut(s) 360
LmnI GCTCC 2 cut(s) 160, 400
LpnPI CCDG 3 cut(s) 68, 215, 306
Lsp1109I GCAGC 4 cut(s) 154, 349, 395, 398
MaeI CTAG 1 cut(s) 282
MaeII ACGT 2 cut(s) 39, 117
MaeIII GTNAC 1 cut(s) 196
MboII GAAGA 4 cut(s) 82, 100, 103, 340
MfeI CAATTG 1 cut(s) 201
MluCI AATT 3 cut(s) 201, 210, 337
MnlI CCTC 9 cut(s) 19, 116, 126, 148, 278, 361, 366, 385, 391
MspA1I CMGCKG 1 cut(s) 359
MunI CAATTG 1 cut(s) 201
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 362, 392
NlaIII CATG 1 cut(s) 253
NmuCI GTSAC 1 cut(s) 196
PagI TCATGA 1 cut(s) 249
PfeI GAWTC 2 cut(s) 59, 278
PkrI GCNGC 6 cut(s) 144, 358, 361, 364, 385, 388
PstNI CAGNNNCTG 1 cut(s) 395
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacII CCGCGG 1 cut(s) 360
SatI GCNGC 6 cut(s) 143, 357, 360, 363, 384, 387
SetI ASST 7 cut(s) 42, 87, 120, 137, 159, 165, 410
Sfr303I CCGCGG 1 cut(s) 360
SgrBI CCGCGG 1 cut(s) 360
Sse9I AATT 3 cut(s) 201, 210, 337
SsiI CCGC 2 cut(s) 357, 359
SspMI CTAG 1 cut(s) 282
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 3 cut(s) 77, 193, 262
TaiI ACGT 2 cut(s) 42, 120
TaqI TCGA 1 cut(s) 342
TasI AATT 3 cut(s) 201, 210, 337
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 359, 362
TfiI GAWTC 2 cut(s) 59, 278
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 4 cut(s) 142, 362, 383, 386
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 47
XapI RAATTY 2 cut(s) 210, 337
XbaI TCTAGA 1 cut(s) 281
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.