pycom09g13850

At2g34160-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002909
Physical Location & Seq
Forward (+)
13000620 .. 13002999
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g13850.5

Sequence Viewer

Length: 396 bp
ATGGAGGAGATCACGGACGGCGTGAACAACGTCACCATCAACGCCTCCGATTCGTACAAGAAGAACCGAATTCAGGTCTCAAATACCAAGAAGCCGCTCTTCTTCTATGTTAATCTTGCCAAGAGGTATATGCAACAGTACAATGAGGTGGAGCTCTCCGCTTTGGGAATGGCTATTGCCACGGTTGTTACAATTGCAGAAATTCTGAAAAACAATGGGCTGGCAGTTGAGAAAAAAATCATGACATCAACTGTTGATATTAAGGATGATTCTAGGGGGAGGCCAGTCCAAAAAGCCAAGATTGAGATAGTACTTGGGAAGTCAGAAAACTTTGATGACTTGATGGCCGCTGCAGCCGAGGAGAGGGAAATGGCAGGTGCTGAGGAGCAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

132

Amino Acids

14.55

Weight (kDa)

5.09

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 365
Acc36I ACCTGC 1 cut(s) 365
AccBSI CCGCTC 1 cut(s) 97
AciI CCGC 3 cut(s) 95, 159, 348
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 69, 201
AfaI GTAC 3 cut(s) 56, 140, 312
AfiI CCNNNNNNNGG 2 cut(s) 73, 363
AluBI AGCT 2 cut(s) 154, 393
AluI AGCT 2 cut(s) 154, 393
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 1 cut(s) 82
AlwNI CAGNNNCTG 1 cut(s) 380
AoxI GGCC 2 cut(s) 281, 345
ApeKI GCWGC 2 cut(s) 350, 353
ApoI RAATTY 2 cut(s) 69, 201
AsuHPI GGTGA 1 cut(s) 25
BanII GRGCYC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 156
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 337, 365
BccI CCATC 2 cut(s) 44, 337
BceAI ACGGC 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 365
BisI GCNGC 4 cut(s) 95, 348, 351, 354
BlsI GCNGC 4 cut(s) 96, 349, 352, 355
BmcAI AGTACT 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 180, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 363
Bse1I ACTGG 1 cut(s) 284
BseDI CCNNGG 2 cut(s) 180, 357
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 2 cut(s) 73, 363
BseMII CTCAG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 2 cut(s) 20, 374
BseXI GCAGC 2 cut(s) 337, 365
BshFI GGCC 2 cut(s) 283, 347
BsiHKAI GWGCWC 1 cut(s) 156
BslI CCNNNNNNNGG 2 cut(s) 73, 363
BsmAI GTCTC 1 cut(s) 82
BsnI GGCC 2 cut(s) 283, 347
Bso31I GGTCTC 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 3 cut(s) 95, 159, 348
BspANI GGCC 2 cut(s) 283, 347
BspCNI CTCAG 1 cut(s) 373
BspHI TCATGA 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 97
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 180, 357
BssMI GATC 1 cut(s) 9
Bst4CI ACNGT 3 cut(s) 138, 184, 253
Bst6I CTCTTC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 381
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 271
BstKTI GATC 1 cut(s) 12
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 337, 365
BsuRI GGCC 2 cut(s) 283, 347
BtgI CCRYGG 1 cut(s) 180
BtsCI GGATG 1 cut(s) 271
BveI ACCTGC 1 cut(s) 365
Cac8I GCNNGC 1 cut(s) 222
CaiI CAGNNNCTG 1 cut(s) 380
CciI TCATGA 1 cut(s) 240
Csp6I GTAC 3 cut(s) 55, 139, 311
CviAII CATG 1 cut(s) 241
CviJI RGCY 9 cut(s) 94, 154, 173, 220, 283, 296, 347, 356, 393
CviKI_1 RGCY 9 cut(s) 94, 154, 173, 220, 283, 296, 347, 356, 393
CviQI GTAC 3 cut(s) 55, 139, 311
DdeI CTNAG 1 cut(s) 381
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 156
FaeI CATG 1 cut(s) 244
FaiI YATR 4 cut(s) 108, 129, 131, 242
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FatI CATG 1 cut(s) 240
Fnu4HI GCNGC 4 cut(s) 95, 348, 351, 354
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 95, 348, 351, 354
FspBI CTAG 1 cut(s) 273
GluI GCNGC 4 cut(s) 95, 348, 351, 354
HaeIII GGCC 2 cut(s) 283, 347
Hin1II CATG 1 cut(s) 244
HinfI GANTC 2 cut(s) 50, 269
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 3 cut(s) 49, 207, 325
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 25
HpyCH4III ACNGT 3 cut(s) 138, 184, 253
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 3 cut(s) 133, 197, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 1 cut(s) 381
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 1 cut(s) 244
Kzo9I GATC 1 cut(s) 9
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 2 cut(s) 151, 385
LpnPI CCDG 4 cut(s) 59, 206, 297, 360
Lsp1109I GCAGC 2 cut(s) 337, 365
MaeI CTAG 1 cut(s) 273
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 2 cut(s) 31, 187
MalI GATC 1 cut(s) 11
MbiI CCGCTC 1 cut(s) 97
MboI GATC 1 cut(s) 9
MboII GAAGA 3 cut(s) 73, 91, 94
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 3 cut(s) 69, 192, 201
MnlI CCTC 7 cut(s) 55, 117, 139, 273, 352, 357, 376
MseI TTAA 2 cut(s) 111, 261
MspA1I CMGCKG 1 cut(s) 350
MunI CAATTG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 353
NdeII GATC 1 cut(s) 9
NlaIII CATG 1 cut(s) 244
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 31
PagI TCATGA 1 cut(s) 240
PaqCI CACCTGC 1 cut(s) 365
PciSI GCTCTTC 1 cut(s) 104
PfeI GAWTC 2 cut(s) 50, 269
PkrI GCNGC 4 cut(s) 96, 349, 352, 355
Psp124BI GAGCTC 1 cut(s) 156
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 380
RsaI GTAC 3 cut(s) 56, 140, 312
RsaNI GTAC 3 cut(s) 55, 139, 311
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 2 cut(s) 111, 261
SatI GCNGC 4 cut(s) 95, 348, 351, 354
Sau3AI GATC 1 cut(s) 9
ScaI AGTACT 1 cut(s) 312
SduI GDGCHC 1 cut(s) 156
SetI ASST 7 cut(s) 33, 78, 128, 150, 156, 379, 395
SfcI CTRYAG 1 cut(s) 351
Sse9I AATT 3 cut(s) 69, 192, 201
SsiI CCGC 3 cut(s) 95, 159, 348
SspMI CTAG 1 cut(s) 273
SstI GAGCTC 1 cut(s) 156
TaaI ACNGT 3 cut(s) 138, 184, 253
TaiI ACGT 1 cut(s) 33
TasI AATT 3 cut(s) 69, 192, 201
TatI WGTACW 2 cut(s) 138, 310
TauI GCSGC 2 cut(s) 97, 350
TfiI GAWTC 2 cut(s) 50, 269
Tru1I TTAA 2 cut(s) 111, 261
Tru9I TTAA 2 cut(s) 111, 261
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 350, 353
Tsp45I GTSAC 1 cut(s) 31
TspGWI ACGGA 1 cut(s) 29
XapI RAATTY 2 cut(s) 69, 201
XspI CTAG 1 cut(s) 273
ZrmI AGTACT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.