MD09G1220100.v1.1

At2g34160-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002909
Physical Location & Seq
Reverse (-)
24970334 .. 24973062
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1220100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGAAGAACAAATTTAGAAAGAGAAATCGGAGAGAGAGTAGTCAAAAGGGATTTAGGGTTAGGGTTTTTGAAGCTGTTCTCTCAATTGCAATGGAGGAGATCACTGACGGCGTGAACAACATCACCATCAACGCCTCCGATTCGCACAAGAAGAACCGAATTCAGGTCTCAAATACCAAGAAGCCGCTCTTCTTCTATGTTAATCTTGCCAAGAGGTATATGCAACAGTACAATGAGGTGGAGCTCTCCGCTTTGGGAATGGCTATTGCCACGGTCGTTACAATTGCAGAAATTCTGAAAAACAATGGGCTGGCAGTTGAGAAAGAAATCATGACATCAACTGTTGACATTAAGGATGATTCCAGGGGGAGGCCAGTCCAAAAAGCCAAGATTGAGATAGTACTTGGGAAGTCAGAAAACTTTGATGATTTGATGGCCGCTGCAGCCGAGGAGAGGGAAATTGCAGGTGCTGAGGAGCAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alba

Protein Analysis

162

Amino Acids

18.14

Weight (kDa)

9.1

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 52 - 113 1.8e-13 Alba
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 187
AciI CCGC 3 cut(s) 185, 249, 438
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 3 cut(s) 11, 159, 291
AfaI GTAC 2 cut(s) 230, 402
AfiI CCNNNNNNNGG 3 cut(s) 163, 369, 453
AgsI TTSAA 1 cut(s) 71
AjnI CCWGG 1 cut(s) 362
AluBI AGCT 3 cut(s) 74, 244, 483
AluI AGCT 3 cut(s) 74, 244, 483
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 172
AlwNI CAGNNNCTG 1 cut(s) 470
AoxI GGCC 2 cut(s) 371, 435
ApeKI GCWGC 2 cut(s) 440, 443
ApoI RAATTY 3 cut(s) 11, 159, 291
Asp700I GAANNNNTTC 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 115
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 427, 455
BccI CCATC 2 cut(s) 134, 427
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 172
BfmI CTRYAG 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 4 cut(s) 185, 438, 441, 444
BlsI GCNGC 4 cut(s) 186, 439, 442, 445
BmcAI AGTACT 1 cut(s) 402
Bme1390I CCNGG 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 364
Bpu10I CCTNAGC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 3 cut(s) 270, 363, 447
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 369, 453
Bse1I ACTGG 1 cut(s) 374
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 364
BseDI CCNNGG 3 cut(s) 270, 363, 447
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 3 cut(s) 163, 369, 453
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 1 cut(s) 374
BseRI GAGGAG 2 cut(s) 110, 464
BseXI GCAGC 2 cut(s) 427, 455
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 2 cut(s) 373, 437
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 246
BslI CCNNNNNNNGG 3 cut(s) 163, 369, 453
BsmAI GTCTC 1 cut(s) 172
BsnI GGCC 2 cut(s) 373, 437
Bso31I GGTCTC 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 1 cut(s) 99
BspACI CCGC 3 cut(s) 185, 249, 438
BspANI GGCC 2 cut(s) 373, 437
BspCNI CTCAG 1 cut(s) 463
BspHI TCATGA 1 cut(s) 330
BspMAI CTGCAG 1 cut(s) 445
BspMI ACCTGC 1 cut(s) 455
BspQI GCTCTTC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 270, 363, 447
BssMI GATC 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 3 cut(s) 228, 274, 343
Bst6I CTCTTC 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 312
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 1 cut(s) 99
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 362
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 427, 455
BsuRI GGCC 2 cut(s) 373, 437
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 312
CaiI CAGNNNCTG 1 cut(s) 470
CciI TCATGA 1 cut(s) 330
Csp6I GTAC 2 cut(s) 229, 401
CviAII CATG 1 cut(s) 331
CviQI GTAC 2 cut(s) 229, 401
DdeI CTNAG 1 cut(s) 471
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 362
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 1 cut(s) 334
FaiI YATR 4 cut(s) 198, 219, 221, 332
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FatI CATG 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 185, 438, 441, 444
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 4 cut(s) 185, 438, 441, 444
GluI GCNGC 4 cut(s) 185, 438, 441, 444
HaeIII GGCC 2 cut(s) 373, 437
Hin1II CATG 1 cut(s) 334
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 2 cut(s) 140, 359
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 115, 346
Hpy188I TCNGA 4 cut(s) 30, 139, 297, 415
Hpy188III TCNNGA 1 cut(s) 331
Hpy8I GTNNAC 2 cut(s) 115, 346
HpyCH4III ACNGT 3 cut(s) 228, 274, 343
HpyCH4V TGCA 5 cut(s) 89, 223, 287, 443, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 471
Hsp92II CATG 1 cut(s) 334
Kzo9I GATC 1 cut(s) 99
LguI GCTCTTC 1 cut(s) 194
LmnI GCTCC 2 cut(s) 241, 475
LpnPI CCDG 6 cut(s) 149, 296, 349, 376, 387, 450
Lsp1109I GCAGC 2 cut(s) 427, 455
MaeIII GTNAC 1 cut(s) 277
MalI GATC 1 cut(s) 101
MbiI CCGCTC 1 cut(s) 187
MboI GATC 1 cut(s) 99
MboII GAAGA 4 cut(s) 16, 163, 181, 184
MfeI CAATTG 2 cut(s) 84, 282
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 6 cut(s) 11, 84, 159, 282, 291, 459
MnlI CCTC 8 cut(s) 88, 145, 207, 229, 363, 442, 447, 466
MroXI GAANNNNTTC 1 cut(s) 75
MseI TTAA 2 cut(s) 201, 351
MspA1I CMGCKG 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 364
MunI CAATTG 2 cut(s) 84, 282
MvaI CCWGG 1 cut(s) 364
MwoI GCNNNNNNNGC 1 cut(s) 443
NdeII GATC 1 cut(s) 99
NlaIII CATG 1 cut(s) 334
NmeAIII GCCGAG 1 cut(s) 472
PagI TCATGA 1 cut(s) 330
PaqCI CACCTGC 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 2 cut(s) 140, 359
PkrI GCNGC 4 cut(s) 186, 439, 442, 445
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 470
RsaI GTAC 2 cut(s) 230, 402
RsaNI GTAC 2 cut(s) 229, 401
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 194
SaqAI TTAA 2 cut(s) 201, 351
SatI GCNGC 4 cut(s) 185, 438, 441, 444
Sau3AI GATC 1 cut(s) 99
ScaI AGTACT 1 cut(s) 402
ScrFI CCNGG 1 cut(s) 364
SduI GDGCHC 1 cut(s) 246
SetI ASST 7 cut(s) 76, 168, 218, 240, 246, 469, 485
SfcI CTRYAG 1 cut(s) 441
Sse9I AATT 6 cut(s) 11, 84, 159, 282, 291, 459
SsiI CCGC 3 cut(s) 185, 249, 438
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 362
TaaI ACNGT 3 cut(s) 228, 274, 343
TasI AATT 6 cut(s) 11, 84, 159, 282, 291, 459
TatI WGTACW 2 cut(s) 228, 400
TauI GCSGC 2 cut(s) 187, 440
TfiI GAWTC 2 cut(s) 140, 359
Tru1I TTAA 2 cut(s) 201, 351
Tru9I TTAA 2 cut(s) 201, 351
TscAI CASTG 1 cut(s) 109
TseI GCWGC 2 cut(s) 440, 443
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 109
XapI RAATTY 3 cut(s) 11, 159, 291
XmnI GAANNNNTTC 1 cut(s) 75
ZrmI AGTACT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.