FvH4_3g02000

Common central domain of tyrosinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000836
Physical Location & Seq
Reverse (-)
1008033 .. 1010382
2350 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02000.t1

Sequence Viewer

Length: 1701 bp
ATGGAAGCAGCAAAAAAATGGGGTTTCGCCGCCATTACCTTGGTGTTCATCATGTTTGCAGCATTGCCTCTGACTCTTCTGGTCCTCAGAGATCCAACTGACTCTTGGAAACTTACAGACACAATCCTAAGCCTACTAAGAATAATTGAATCAAAAGATTCTTCAGGAAGCTACAAGATAGACAGAGGAGACCTCTCAGTGACTCTTAACTTAAGTAGTTGTCACTCATCTTATGGAAGGCCAGATCTTCTTGTCTATTGTTGTCCCCCAAAAAGTCTAGCAGAAGAACCCGTCATTGATTTCCAATTTCCAGATCCTTCAACCCCTCTGAGAATTCGGAGGCCAGCACACCGGGTTGGTCGTGACTTCATTGCCAAATACAAAAAGGCTCTCTTGATCATGAAGTCACTTCCATACAATGACCCTCGGAGTTTTCGTCGTCAAGCTGATATGCATTGCCTCTTCTGCACCGGTGCTTATGACCAACCACATTCCCATCGCCCTGTCGACATCCATCAAAAATGGTTCTTTTTCCCATGGCACCGGATGCTGCTTCACTTCCATGAACGCATCGTTGGAAGCCTCATTGGTGATGACACATTTGCTCTGCCATTTTGGAATTGGGACTCCCCTGATGGAATGGCAATGCCCGAGTGGTATATGCACTCGCCTTTCTTTGACGATCAGAGGGACGTTTCTCACTTCCCACCAAAAGTAAGTGATTTAAACTACAATTACGAGAAGACAGATGAGAATATGGAAAATAAAGAGCGAATGCACTTGAACTTGGTGTTCATGTATAACCAGATGGTGTCTGCAGCAAAGAAGCCCGAGCTGTTCATGGGGTGCCCTTATAAGAATAGCGAGGAGGGTGTTTGTGATGGACTTGGAACACTAGAAAATGCTCCTCACAACACAATGCACACTTGGGTTGGAAGCAATTTCAATGTCAGGAGGGAAGACATGGGAGCATTTTACTCTGCAGCTAGGGACCCTAGTTTCTATGCACACCACTCAAATCTAGACCGTCTTTGGGAGGTTTGGACGCAAATTCACAAGGTCGACTTGCAAACATTTGATTCTGATTGGCTCAACTCTAGTTTCTACTTCCATGATGAAAATTCACAGCTTGTGAGAATCAAAGTTCGTGATGTTTTGGATACTACAAAGTTGAGGTATGTTTACGAGGAGGTTGATCTTCCATGGCTGAATACGCGTCCAAAGCCTAACTCTATTCCATCCAAAATTGCCAACCATGCACTGAAAATAAGAGAAAACATAACCATTTTGTCTGCAGAGATGAGACCAGGCGGTCAGAATCTCAATAGCTCCATTACAGTGAGGGTGAGCAGGTCTAAATTTAACAGAAGGAAGGTAGAGAAAGATGTAGAGGAAGAGGTTTTGGTTGTTCATGGAATTGCTGTGAGAATTGATGAGTATGTCAAATTTGATGTGTATATAAATGTAGTCAATGAGAGCAAAATGGGACCGAGTTTCAGGGAATTTGCCGGAACCTTTGTCCACATACCTCATGGAAAGATGGATGGTGACCAAAAGACCAATCTGAAGTTTGGTATCTCGGAATTGCTTGAAGATTTGGAGGCAGATAGAGATGAGAGCATTTTGGTCACATTGCTACCTAGGACTAAATCATGCACTAACACAACAATTGATGGGATTCGGATTGAATACATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110
Pfam Domains
Protein Families
PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

567

Amino Acids

65.65

Weight (kDa)

6.35

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tyrosinase PF00264 143 - 350 2.3e-30 Common central domain of tyrosinase
PPO1_DWL PF12142 360 - 408 7.4e-25 Polyphenol oxidase middle domain
PPO1_KFDV PF12143 440 - 564 1.7e-37 Chloroplastic Polyphenol Oxidase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AasI GACNNNNNNGTC 1 cut(s) 1311
Acc36I ACCTGC 1 cut(s) 1341
AccB1I GGYRCC 2 cut(s) 540, 846
AccI GTMKAC 2 cut(s) 507, 1062
AccII CGCG 1 cut(s) 1216
AciI CCGC 2 cut(s) 30, 1311
AclWI GGATC 2 cut(s) 86, 308
AcsI RAATTY 6 cut(s) 333, 1050, 1120, 1358, 1445, 1502
AcuI CTGAAG 2 cut(s) 147, 1586
AfiI CCNNNNNNNGG 1 cut(s) 1033
AflII CTTAAG 1 cut(s) 211
AflIII ACRYGT 1 cut(s) 1214
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 6 cut(s) 149, 321, 784, 946, 1592, 1688
AjnI CCWGG 1 cut(s) 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 558, 590
AluBI AGCT 6 cut(s) 171, 446, 835, 986, 1129, 1329
AluI AGCT 6 cut(s) 171, 446, 835, 986, 1129, 1329
Alw26I GTCTC 2 cut(s) 183, 1297
AlwI GGATC 2 cut(s) 86, 308
Ama87I CYCGRG 2 cut(s) 650, 830
AoxI GGCC 2 cut(s) 239, 341
ApeKI GCWGC 5 cut(s) 8, 59, 550, 818, 983
ApoI RAATTY 6 cut(s) 333, 1050, 1120, 1358, 1445, 1502
AsiGI ACCGGT 1 cut(s) 470
AspA2I CCTAGG 1 cut(s) 1640
AspS9I GGNCC 3 cut(s) 82, 991, 1487
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 602, 1357, 1559
AvaI CYCGRG 2 cut(s) 650, 830
AvaII GGWCC 3 cut(s) 82, 991, 1487
AvrII CCTAGG 1 cut(s) 1640
BaeGI GKGCMC 1 cut(s) 851
BanI GGYRCC 2 cut(s) 540, 846
BbsI GAAGAC 2 cut(s) 749, 966
BbvI GCAGC 5 cut(s) 20, 71, 537, 830, 995
BccI CCATC 9 cut(s) 504, 522, 629, 802, 875, 1246, 1534, 1538, 1667
BciT130I CCWGG 1 cut(s) 1308
BciVI GTATCC 1 cut(s) 1153
BclI TGATCA 1 cut(s) 396
BcnI CCSGG 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 183, 1297
BfaI CTAG 7 cut(s) 278, 896, 987, 996, 1022, 1098, 1641
BfmI CTRYAG 3 cut(s) 816, 981, 1293
BfrI CTTAAG 1 cut(s) 211
BfuAI ACCTGC 1 cut(s) 1341
BfuI GTATCC 1 cut(s) 1153
BglII AGATCT 1 cut(s) 244
BisI GCNGC 6 cut(s) 9, 30, 60, 551, 819, 984
BlnI CCTAGG 1 cut(s) 1640
BlsI GCNGC 6 cut(s) 10, 31, 61, 552, 820, 985
Bme1390I CCNGG 2 cut(s) 353, 1308
Bme18I GGWCC 3 cut(s) 82, 991, 1487
BmeT110I CYCGRG 2 cut(s) 650, 830
BmgT120I GGNCC 3 cut(s) 82, 991, 1487
BmiI GGNNCC 6 cut(s) 542, 848, 992, 993, 1488, 1513
BmrFI CCNGG 2 cut(s) 353, 1308
BmsI GCATC 2 cut(s) 537, 579
BpiI GAAGAC 2 cut(s) 749, 966
BplI GAGNNNNNCTC 2 cut(s) 177, 209
Bpu10I CCTNAGC 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 353
BsaI GGTCTC 2 cut(s) 183, 1297
BsaJI CCNNGG 5 cut(s) 39, 425, 536, 1202, 1640
BsaWI WCCGGW 2 cut(s) 470, 543
BsaXI ACNNNNNCTCC 2 cut(s) 421, 451
Bsc4I CCNNNNNNNGG 1 cut(s) 1033
Bse118I RCCGGY 1 cut(s) 470
Bse3DI GCAATG 5 cut(s) 62, 369, 454, 651, 1631
BseBI CCWGG 1 cut(s) 1308
BseDI CCNNGG 5 cut(s) 39, 425, 536, 1202, 1640
BseGI GGATG 4 cut(s) 510, 552, 1238, 1549
BseLI CCNNNNNNNGG 1 cut(s) 1033
BseMI GCAATG 5 cut(s) 62, 369, 454, 651, 1631
BseMII CTCAG 3 cut(s) 100, 210, 320
BseRI GAGGAG 4 cut(s) 201, 881, 897, 1202
BseSI GKGCMC 1 cut(s) 851
BseXI GCAGC 5 cut(s) 20, 71, 537, 830, 995
BsgI GTGCAG 1 cut(s) 451
Bsh1236I CGCG 1 cut(s) 1216
BshFI GGCC 2 cut(s) 241, 343
BshNI GGYRCC 2 cut(s) 540, 846
BshTI ACCGGT 1 cut(s) 470
BsiHKCI CYCGRG 2 cut(s) 650, 830
BsiSI CCGG 4 cut(s) 352, 471, 544, 1509
BslFI GGGAC 5 cut(s) 249, 638, 704, 1004, 1500
BslI CCNNNNNNNGG 1 cut(s) 1033
BsmAI GTCTC 2 cut(s) 183, 1297
BsmFI GGGAC 5 cut(s) 249, 638, 704, 1004, 1500
BsmI GAATGC 1 cut(s) 780
BsnI GGCC 2 cut(s) 241, 343
Bso31I GGTCTC 2 cut(s) 183, 1297
BsoBI CYCGRG 2 cut(s) 650, 830
Bsp1286I GDGCHC 1 cut(s) 851
Bsp143I GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
Bsp19I CCATGG 2 cut(s) 536, 1202
BspACI CCGC 2 cut(s) 30, 1311
BspANI GGCC 2 cut(s) 241, 343
BspCNI CTCAG 3 cut(s) 99, 209, 321
BspFNI CGCG 1 cut(s) 1216
BspHI TCATGA 1 cut(s) 399
BspLI GGNNCC 6 cut(s) 542, 848, 992, 993, 1488, 1513
BspMAI CTGCAG 3 cut(s) 820, 985, 1297
BspMI ACCTGC 1 cut(s) 1341
BspPI GGATC 2 cut(s) 86, 308
BspT107I GGYRCC 2 cut(s) 540, 846
BspTI CTTAAG 1 cut(s) 211
BspTNI GGTCTC 2 cut(s) 183, 1297
BsrDI GCAATG 5 cut(s) 62, 369, 454, 651, 1631
BsrFI RCCGGY 1 cut(s) 470
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 5 cut(s) 39, 425, 536, 1202, 1640
BssMI GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
BssT1I CCWWGG 4 cut(s) 39, 536, 1202, 1640
Bst2UI CCWGG 1 cut(s) 1308
Bst4CI ACNGT 2 cut(s) 1028, 1339
Bst6I CTCTTC 3 cut(s) 81, 467, 1389
BstAFI CTTAAG 1 cut(s) 211
BstAPI GCANNNNNTGC 1 cut(s) 547
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 5 cut(s) 86, 128, 137, 196, 329
BstDSI CCRYGG 2 cut(s) 536, 1202
BstEII GGTNACC 1 cut(s) 1547
BstF5I GGATG 4 cut(s) 510, 552, 1238, 1549
BstFNI CGCG 1 cut(s) 1216
BstKTI GATC 6 cut(s) 94, 247, 316, 399, 685, 1198
BstMAI GTCTC 2 cut(s) 183, 1297
BstMBI GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
BstMWI GCNNNNNNNGC 5 cut(s) 465, 547, 1213, 1222, 1256
BstNI CCWGG 1 cut(s) 1308
BstPI GGTNACC 1 cut(s) 1547
BstSCI CCNGG 2 cut(s) 351, 1306
BstSFI CTRYAG 3 cut(s) 816, 981, 1293
BstSLI GKGCMC 1 cut(s) 851
BstUI CGCG 1 cut(s) 1216
BstV1I GCAGC 5 cut(s) 20, 71, 537, 830, 995
BstV2I GAAGAC 2 cut(s) 749, 966
BstX2I RGATCY 3 cut(s) 91, 244, 313
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 3 cut(s) 91, 244, 313
BsuI GTATCC 1 cut(s) 1153
BsuRI GGCC 2 cut(s) 241, 343
BtgI CCRYGG 2 cut(s) 536, 1202
BtgZI GCGATG 1 cut(s) 482
BtsCI GGATG 4 cut(s) 510, 552, 1238, 1549
BtsIMutI CAGTG 3 cut(s) 204, 1259, 1344
BveI ACCTGC 1 cut(s) 1341
Cac8I GCNNGC 1 cut(s) 345
CciI TCATGA 1 cut(s) 399
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 3 cut(s) 82, 991, 1487
CseI GACGC 2 cut(s) 1054, 1205
CspAI ACCGGT 1 cut(s) 470
DdeI CTNAG 5 cut(s) 86, 128, 137, 196, 329
DpnI GATC 6 cut(s) 93, 246, 315, 398, 684, 1197
DpnII GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
DraI TTTAAA 1 cut(s) 726
DrdI GACNNNNNNGTC 1 cut(s) 1311
DseDI GACNNNNNNGTC 1 cut(s) 1311
Eam1104I CTCTTC 3 cut(s) 81, 467, 1389
EarI CTCTTC 3 cut(s) 81, 467, 1389
Eco130I CCWWGG 4 cut(s) 39, 536, 1202, 1640
Eco31I GGTCTC 2 cut(s) 183, 1297
Eco47I GGWCC 3 cut(s) 82, 991, 1487
Eco57I CTGAAG 2 cut(s) 147, 1586
Eco88I CYCGRG 2 cut(s) 650, 830
Eco91I GGTNACC 1 cut(s) 1547
EcoO109I RGGNCCY 1 cut(s) 991
EcoO65I GGTNACC 1 cut(s) 1547
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 1306
EcoT14I CCWWGG 4 cut(s) 39, 536, 1202, 1640
EcoT22I ATGCAT 1 cut(s) 456
ErhI CCWWGG 4 cut(s) 39, 536, 1202, 1640
FalI AAGNNNNNCTT 4 cut(s) 377, 409, 1049, 1081
FaqI GGGAC 5 cut(s) 249, 638, 704, 1004, 1500
FbaI TGATCA 1 cut(s) 396
FblI GTMKAC 2 cut(s) 507, 1062
Fnu4HI GCNGC 6 cut(s) 9, 30, 60, 551, 819, 984
FokI GGATG 4 cut(s) 497, 559, 1225, 1556
Fsp4HI GCNGC 6 cut(s) 9, 30, 60, 551, 819, 984
FspBI CTAG 7 cut(s) 278, 896, 987, 996, 1022, 1098, 1641
GluI GCNGC 6 cut(s) 9, 30, 60, 551, 819, 984
HaeIII GGCC 2 cut(s) 241, 343
HapII CCGG 4 cut(s) 352, 471, 544, 1509
HgaI GACGC 2 cut(s) 1054, 1205
HincII GTYRAC 2 cut(s) 508, 1063
HindII GTYRAC 2 cut(s) 508, 1063
HpaII CCGG 4 cut(s) 352, 471, 544, 1509
HphI GGTGA 3 cut(s) 602, 1357, 1559
Hpy166II GTNNAC 4 cut(s) 508, 1063, 1183, 1522
Hpy188III TCNNGA 8 cut(s) 165, 311, 362, 394, 400, 952, 1022, 1148
Hpy8I GTNNAC 4 cut(s) 508, 1063, 1183, 1522
Hpy99I CGWCG 1 cut(s) 441
HpyAV CCTTC 4 cut(s) 231, 327, 1362, 1366
HpyCH4III ACNGT 2 cut(s) 1028, 1339
HpyCH4IV ACGT 1 cut(s) 693
HpyF10VI GCNNNNNNNGC 5 cut(s) 465, 547, 1213, 1222, 1256
HpyF3I CTNAG 5 cut(s) 86, 128, 137, 196, 329
HpySE526I ACGT 1 cut(s) 693
KflI GGGWCCC 1 cut(s) 991
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
LmnI GCTCC 3 cut(s) 910, 968, 1334
Lsp1109I GCAGC 5 cut(s) 20, 71, 537, 830, 995
LweI GCATC 2 cut(s) 537, 579
MaeI CTAG 7 cut(s) 278, 896, 987, 996, 1022, 1098, 1641
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 6 cut(s) 199, 221, 362, 405, 1547, 1627
MalI GATC 6 cut(s) 93, 246, 315, 398, 684, 1197
MboI GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
MfeI CAATTG 1 cut(s) 1668
MflI RGATCY 3 cut(s) 91, 244, 313
MhlI GDGCHC 1 cut(s) 851
MluI ACGCGT 1 cut(s) 1214
MlyI GAGTC 4 cut(s) 67, 95, 196, 620
MmeI TCCRAC 3 cut(s) 119, 556, 913
Mph1103I ATGCAT 1 cut(s) 456
MseI TTAA 4 cut(s) 207, 212, 725, 1362
MslI CAYNNNNRTG 2 cut(s) 561, 1337
MspCI CTTAAG 1 cut(s) 211
MspI CCGG 4 cut(s) 352, 471, 544, 1509
MspR9I CCNGG 2 cut(s) 353, 1308
MunI CAATTG 1 cut(s) 1668
Mva1269I GAATGC 1 cut(s) 780
MvaI CCWGG 1 cut(s) 1308
MvnI CGCG 1 cut(s) 1216
MwoI GCNNNNNNNGC 5 cut(s) 465, 547, 1213, 1222, 1256
NciI CCSGG 1 cut(s) 353
NcoI CCATGG 2 cut(s) 536, 1202
NdeII GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
NlaIV GGNNCC 6 cut(s) 542, 848, 992, 993, 1488, 1513
NmuCI GTSAC 6 cut(s) 199, 221, 362, 405, 1547, 1627
NsiI ATGCAT 1 cut(s) 456
PagI TCATGA 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 433
PctI GAATGC 1 cut(s) 780
PfeI GAWTC 6 cut(s) 149, 158, 1079, 1137, 1318, 1678
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 6 cut(s) 10, 31, 61, 552, 820, 985
PleI GAGTC 4 cut(s) 67, 95, 196, 620
PpsI GAGTC 4 cut(s) 67, 95, 196, 620
PpuMI RGGWCCY 1 cut(s) 991
PsiI TTATAA 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 991
Psp6I CCWGG 1 cut(s) 1306
PspEI GGTNACC 1 cut(s) 1547
PspGI CCWGG 1 cut(s) 1306
PspN4I GGNNCC 6 cut(s) 542, 848, 992, 993, 1488, 1513
PspPI GGNCC 3 cut(s) 82, 991, 1487
PspPPI RGGWCCY 1 cut(s) 991
PstI CTGCAG 3 cut(s) 820, 985, 1297
PsuI RGATCY 3 cut(s) 91, 244, 313
RseI CAYNNNNRTG 2 cut(s) 561, 1337
SalI GTCGAC 2 cut(s) 506, 1061
SaqAI TTAA 4 cut(s) 207, 212, 725, 1362
SatI GCNGC 6 cut(s) 9, 30, 60, 551, 819, 984
Sau3AI GATC 6 cut(s) 91, 244, 313, 396, 682, 1195
Sau96I GGNCC 3 cut(s) 82, 991, 1487
SchI GAGTC 4 cut(s) 67, 95, 196, 620
ScrFI CCNGG 2 cut(s) 353, 1308
SduI GDGCHC 1 cut(s) 851
SfaNI GCATC 2 cut(s) 537, 579
SfcI CTRYAG 3 cut(s) 816, 981, 1293
SgrAI CRCCGGYG 1 cut(s) 470
SinI GGWCC 3 cut(s) 82, 991, 1487
SmiMI CAYNNNNRTG 2 cut(s) 561, 1337
SmlI CTYRAG 1 cut(s) 211
SmoI CTYRAG 1 cut(s) 211
SsiI CCGC 2 cut(s) 30, 1311
SspMI CTAG 7 cut(s) 278, 896, 987, 996, 1022, 1098, 1641
StyD4I CCNGG 2 cut(s) 351, 1306
StyI CCWWGG 4 cut(s) 39, 536, 1202, 1640
TaaI ACNGT 2 cut(s) 1028, 1339
TaiI ACGT 1 cut(s) 696
TaqI TCGA 2 cut(s) 507, 1062
TaqII GACCGA 1 cut(s) 1504
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 6 cut(s) 149, 158, 1079, 1137, 1318, 1678
Tru1I TTAA 4 cut(s) 207, 212, 725, 1362
Tru9I TTAA 4 cut(s) 207, 212, 725, 1362
TscAI CASTG 3 cut(s) 204, 1266, 1344
TseFI GTSAC 6 cut(s) 199, 221, 362, 405, 1547, 1627
TseI GCWGC 5 cut(s) 8, 59, 550, 818, 983
Tsp45I GTSAC 6 cut(s) 199, 221, 362, 405, 1547, 1627
TspDTI ATGAA 8 cut(s) 37, 358, 416, 579, 784, 829, 1131, 1400
TspRI CASTG 3 cut(s) 204, 1266, 1344
Vha464I CTTAAG 1 cut(s) 211
VpaK11BI GGWCC 3 cut(s) 82, 991, 1487
XapI RAATTY 6 cut(s) 333, 1050, 1120, 1358, 1445, 1502
XbaI TCTAGA 1 cut(s) 1021
XcmI CCANNNNNNNNNTGG 3 cut(s) 102, 618, 1529
XmaJI CCTAGG 1 cut(s) 1640
XmiI GTMKAC 2 cut(s) 507, 1062
XspI CTAG 7 cut(s) 278, 896, 987, 996, 1022, 1098, 1641
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.