Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Reverse (-)

89802608 .. 89805167

Gene Structure 2560 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00068700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1674 bp

ATGGCTTCTCTATCACCTCCACTAGTCACCACAACAACCATTTCCATTTCAAATTCCTTCTCTTCTTTCTCTCAAACCAGTTCCCAAATTTCCTTAGTCCAAAACCCTAGGCTTTCCTTAATACGTACCCGGATTTTATGCAAAGCCACAAACAGTAATCAAAACCCTAGCGATGAACAAAAACGTCCCCAAAAAAACCTAGACCGGAGAAATGTGCTCATTGGGCTCGGAGGCCTATACGGTGTTGGTCTTGGTGGCGCCGACCCTCTCGCCTTTGCAAAGCCGGTGGCTCCACCGGACCTATCCAAGTGCGGAGCAGCAGACTTACCAACCGGTGCTCAACCAACAAATTGCTGCCCTCCAATATCTGACAAGATAGTCGACTTCACCTTTCCATCGTCCTCTCCACTCCGCATCAGACCGGCAGCTCACGCCGTTGATCAGGCCTATATCGATAAATACTCCAAGGCCATTGAGCTCATGAAAGCCCTCCCCGACTCCGATCCACGCAGCTTTACCCAACAAGCCGATATTCACTGCGCTTACTGTGACGGCGCGTATGATCAAGTCAGTTTTCCTGATCTCGAGCTCCAAATCCACCAGTCATGGCTGTTCTTCCCCTTCCACAGATACTACTTATATTTCTACGAGAGAATTCTCGGAAAACTCATCGGCGACCCCACATTCGCATTGCCATTTTGGAACTGGGACTCCCCTCCGGGAATGAAGCTGCCAGCCCTCTTCGCTGATCCAAGCTCGCCGCTCTACGATGAGCTCCGAAACGCCAACCACCAGCCTCCAACGCTCCTTGACCTAGACTGGAACCAGACCGACATACCGACAACCGAGGCTGACCAAATATCAAGCAATTTGAAGATTATGTACAGGCAGATGGTGTCCAACGCCAAGAAACAGCGGCTCTTCTTTGGTCAAGCTTACAGGGCTGGCACCGATCCAGATCCTGGAGCCGGCTCAATCGAGACTACTCCTCACGGGCCAATCCACGTCTGGACTGGTGATAACACCCAGCCCAATTACGAGGACATGGGGAACTTCTACTCTGCTGCTAGAGACCCTATTTTCTTCTCACACCATTCTAATGTCGATAGGATGTGGAGTGTATGGAAAACATTGGGTAAGAAGAATAAGGATATTACCGATCCAGATTGGTTGGACACAGGGTTCCTATTCTATGACGAGAACGCGCAGCTTGTGCGCGTGAAGGTACGCGACTGCCTTGACTCCAAAAAGCTTGGGTATGTTTACCAAGATGTGGACATTCCATGGTTGACGTCCAAACCAACACCGCGTAGGTCGAAGCTTGCGAAAGCAGCCAAGGCCATTGGGGTGGCGCGTGCAGCGACCTCCGGACCAAAGGTGGTGGGAAGAGGCGATTTTCCAATAAAGTTGGACTCGAAGATTAGGACTGTGGTAACGAGGCCAAAGCAGAAGAAGAGGAGTAAGAAGGAGAAGGAGGACGAGGAGGAGATACTTGTGATTGAAGGGATCGAGTTTGATAAAGATGTGGCGGTGAAGTTTGATGTGTACGTTAATGACTTGGAAGACGATGATGAGCCGGGCAAGCCGGACGACAGTGAGTTTGCCGGGAGCTTCGTGAACGTGCCCCATAAGCATAAGGAGAAGAAGAAAAGAAGAAGACTACGAGTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

557

Amino Acids

62.52

Weight (kDa)

7.99

Isoelectric Point (pI)

44.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	170 - 377	9.6e-38	Common central domain of tyrosinase
PPO1_DWL	PF12142	384 - 435	2.1e-28	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	466 - 553	7e-31	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	377
AatII	GACGTC	1 cut(s)	1295
AccB1I	GGYRCC	2 cut(s)	257, 947
AccB7I	CCANNNNNTGG	2 cut(s)	962, 1273
AccBSI	CCGCTC	1 cut(s)	763
AccI	GTMKAC	1 cut(s)	381
AccII	CGCG	6 cut(s)	557, 1205, 1218, 1230, 1309, 1354
AccIII	TCCGGA	1 cut(s)	1367
AciI	CCGC	6 cut(s)	312, 412, 761, 916, 1307, 1529
AclWI	GGATC	6 cut(s)	497, 743, 947, 953, 1154, 1514
AcsI	RAATTY	3 cut(s)	52, 87, 654
AcyI	GRCGYC	2 cut(s)	258, 1292
AfaI	GTAC	4 cut(s)	127, 884, 1227, 1547
AfiI	CCNNNNNNNGG	3 cut(s)	962, 968, 1273
AgeI	ACCGGT	1 cut(s)	332
AgsI	TTSAA	3 cut(s)	51, 874, 1502
AhlI	ACTAGT	1 cut(s)	22
AjiI	CACGTC	1 cut(s)	1006
AjnI	CCWGG	1 cut(s)	961
AloI	GAACNNNNNNTCC	4 cut(s)	695, 727, 1166, 1198
Alw21I	GWGCWC	5 cut(s)	219, 340, 480, 591, 777
Alw26I	GTCTC	2 cut(s)	974, 1065
AlwI	GGATC	6 cut(s)	497, 743, 947, 953, 1154, 1514
AlwNI	CAGNNNCTG	1 cut(s)	962
Ama87I	CYCGRG	1 cut(s)	584
Aor13HI	TCCGGA	1 cut(s)	1367
AoxI	GGCC	6 cut(s)	232, 444, 468, 995, 1338, 1439
ApeKI	GCWGC	9 cut(s)	317, 354, 425, 510, 730, 1064, 1207, 1331, 1358
ApoI	RAATTY	3 cut(s)	52, 87, 654
ArsI	GACNNNNNNTTYG	4 cut(s)	668, 700, 1583, 1615
AsiGI	ACCGGT	1 cut(s)	332
AspA2I	CCTAGG	1 cut(s)	107
AspLEI	GCGC	6 cut(s)	260, 542, 557, 1207, 1218, 1354
AspS9I	GGNCC	3 cut(s)	298, 995, 1370
AsuC2I	CCSGG	4 cut(s)	130, 720, 1578, 1606
AsuHPI	GGTGA	5 cut(s)	6, 19, 379, 1028, 1543
AvaI	CYCGRG	1 cut(s)	584
AvaII	GGWCC	2 cut(s)	298, 1370
AvrII	CCTAGG	1 cut(s)	107
BaeGI	GKGCMC	1 cut(s)	1626
BanI	GGYRCC	2 cut(s)	257, 947
BanII	GRGCYC	4 cut(s)	228, 480, 591, 777
BarI	GAAGNNNNNNTAC	2 cut(s)	866, 898
BbsI	GAAGAC	2 cut(s)	1569, 1663
Bbv12I	GWGCWC	5 cut(s)	219, 340, 480, 591, 777
BbvI	GCAGC	9 cut(s)	329, 341, 437, 522, 717, 1051, 1219, 1343, 1370
BccI	CCATC	2 cut(s)	403, 886
BceAI	ACGGC	2 cut(s)	419, 568
BciT130I	CCWGG	1 cut(s)	963
BclI	TGATCA	2 cut(s)	439, 562
BcnI	CCSGG	4 cut(s)	130, 720, 1578, 1606
BcoDI	GTCTC	2 cut(s)	974, 1065
BcuI	ACTAGT	1 cut(s)	22
BfaI	CTAG	6 cut(s)	23, 108, 168, 200, 815, 1068
BfoI	RGCGCY	1 cut(s)	261
BlnI	CCTAGG	1 cut(s)	107
Bme1390I	CCNGG	5 cut(s)	130, 720, 963, 1578, 1606
Bme18I	GGWCC	2 cut(s)	298, 1370
BmeT110I	CYCGRG	1 cut(s)	584
BmgBI	CACGTC	1 cut(s)	1006
BmgT120I	GGNCC	3 cut(s)	298, 995, 1370
BmiI	GGNNCC	6 cut(s)	259, 291, 824, 949, 967, 1184
BmrFI	CCNGG	5 cut(s)	130, 720, 963, 1578, 1606
BmrI	ACTGGG	1 cut(s)	715
BmsI	GCATC	1 cut(s)	423
BmuI	ACTGGG	1 cut(s)	715
BpiI	GAAGAC	2 cut(s)	1569, 1663
BpmI	CTGGAG	1 cut(s)	984
BpuMI	CCSGG	4 cut(s)	130, 720, 1578, 1606
Bsa29I	ATCGAT	1 cut(s)	453
BsaAI	YACGTR	1 cut(s)	125
BsaBI	GATNNNNATC	1 cut(s)	957
BsaHI	GRCGYC	2 cut(s)	258, 1292
BsaI	GGTCTC	1 cut(s)	1065
BsaJI	CCNNGG	5 cut(s)	107, 465, 846, 1283, 1335
BsaWI	WCCGGW	4 cut(s)	204, 295, 332, 1367
BsaXI	ACNNNNNCTCC	2 cut(s)	695, 725
Bsc4I	CCNNNNNNNGG	3 cut(s)	962, 968, 1273
Bse118I	RCCGGY	4 cut(s)	283, 332, 421, 968
Bse1I	ACTGG	5 cut(s)	78, 601, 710, 824, 1018
Bse3DI	GCAATG	1 cut(s)	689
Bse8I	GATNNNNATC	1 cut(s)	957
BseAI	TCCGGA	1 cut(s)	1367
BseBI	CCWGG	1 cut(s)	963
BseCI	ATCGAT	1 cut(s)	453
BseDI	CCNNGG	5 cut(s)	107, 465, 846, 1283, 1335
BseGI	GGATG	1 cut(s)	1116
BseJI	GATNNNNATC	1 cut(s)	957
BseLI	CCNNNNNNNGG	3 cut(s)	962, 968, 1273
BseMI	GCAATG	1 cut(s)	689
BseNI	ACTGG	5 cut(s)	78, 601, 710, 824, 1018
BseRI	GAGGAG	4 cut(s)	978, 1471, 1496, 1499
BseSI	GKGCMC	1 cut(s)	1626
BseXI	GCAGC	9 cut(s)	329, 341, 437, 522, 717, 1051, 1219, 1343, 1370
BseYI	CCCAGC	1 cut(s)	1026
BsgI	GTGCAG	1 cut(s)	1377
Bsh1236I	CGCG	6 cut(s)	557, 1205, 1218, 1230, 1309, 1354
BshFI	GGCC	6 cut(s)	234, 446, 470, 997, 1340, 1441
BshNI	GGYRCC	2 cut(s)	257, 947
BshTI	ACCGGT	1 cut(s)	332
BshVI	ATCGAT	1 cut(s)	453
BsiHKAI	GWGCWC	5 cut(s)	219, 340, 480, 591, 777
BsiHKCI	CYCGRG	1 cut(s)	584
BslFI	GGGAC	2 cut(s)	171, 722
BslI	CCNNNNNNNGG	3 cut(s)	962, 968, 1273
BsmAI	GTCTC	2 cut(s)	974, 1065
BsmFI	GGGAC	2 cut(s)	171, 722
BsnI	GGCC	6 cut(s)	234, 446, 470, 997, 1340, 1441
Bso31I	GGTCTC	1 cut(s)	1065
BsoBI	CYCGRG	1 cut(s)	584
Bsp1286I	GDGCHC	7 cut(s)	219, 228, 340, 480, 591, 777, 1626
Bsp13I	TCCGGA	1 cut(s)	1367
Bsp1407I	TGTACA	1 cut(s)	882
Bsp143I	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
Bsp19I	CCATGG	1 cut(s)	1283
BspACI	CCGC	6 cut(s)	312, 412, 761, 916, 1307, 1529
BspANI	GGCC	6 cut(s)	234, 446, 470, 997, 1340, 1441
BspDI	ATCGAT	1 cut(s)	453
BspEI	TCCGGA	1 cut(s)	1367
BspFNI	CGCG	6 cut(s)	557, 1205, 1218, 1230, 1309, 1354
BspHI	TCATGA	1 cut(s)	480
BspLI	GGNNCC	6 cut(s)	259, 291, 824, 949, 967, 1184
BspPI	GGATC	6 cut(s)	497, 743, 947, 953, 1154, 1514
BspQI	GCTCTTC	1 cut(s)	926
BspT107I	GGYRCC	2 cut(s)	257, 947
BspTNI	GGTCTC	1 cut(s)	1065
BsrBI	CCGCTC	1 cut(s)	763
BsrDI	GCAATG	1 cut(s)	689
BsrFI	RCCGGY	4 cut(s)	283, 332, 421, 968
BsrGI	TGTACA	1 cut(s)	882
BsrI	ACTGG	5 cut(s)	78, 601, 710, 824, 1018
BssAI	RCCGGY	4 cut(s)	283, 332, 421, 968
BssECI	CCNNGG	5 cut(s)	107, 465, 846, 1283, 1335
BssMI	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
BssNI	GRCGYC	2 cut(s)	258, 1292
BssT1I	CCWWGG	4 cut(s)	107, 465, 1283, 1335
Bst2UI	CCWGG	1 cut(s)	963
Bst4CI	ACNGT	5 cut(s)	155, 242, 548, 1429, 1595
Bst6I	CTCTTC	5 cut(s)	67, 746, 926, 1381, 1448
BstACI	GRCGYC	2 cut(s)	258, 1292
BstAPI	GCANNNNNTGC	1 cut(s)	1213
BstAUI	TGTACA	1 cut(s)	882
BstBAI	YACGTR	1 cut(s)	125
BstC8I	GCNNGC	7 cut(s)	735, 758, 946, 970, 1323, 1356, 1583
BstDEI	CTNAG	1 cut(s)	94
BstDSI	CCRYGG	1 cut(s)	1283
BstF5I	GGATG	1 cut(s)	1116
BstFNI	CGCG	6 cut(s)	557, 1205, 1218, 1230, 1309, 1354
BstH2I	RGCGCY	1 cut(s)	261
BstHHI	GCGC	6 cut(s)	260, 542, 557, 1207, 1218, 1354
BstKTI	GATC	9 cut(s)	442, 505, 565, 583, 751, 955, 961, 1162, 1509
BstMAI	GTCTC	2 cut(s)	974, 1065
BstMBI	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
BstNI	CCWGG	1 cut(s)	963
BstSCI	CCNGG	5 cut(s)	128, 718, 961, 1576, 1604
BstSLI	GKGCMC	1 cut(s)	1626
BstSNI	TACGTA	1 cut(s)	125
BstUI	CGCG	6 cut(s)	557, 1205, 1218, 1230, 1309, 1354
BstV1I	GCAGC	9 cut(s)	329, 341, 437, 522, 717, 1051, 1219, 1343, 1370
BstV2I	GAAGAC	2 cut(s)	1569, 1663
BstX2I	RGATCY	1 cut(s)	958
BstXI	CCANNNNNNTGG	1 cut(s)	1348
BstYI	RGATCY	1 cut(s)	958
Bsu15I	ATCGAT	1 cut(s)	453
BsuRI	GGCC	6 cut(s)	234, 446, 470, 997, 1340, 1441
BsuTUI	ATCGAT	1 cut(s)	453
BtgI	CCRYGG	1 cut(s)	1283
BtgZI	GCGATG	1 cut(s)	186
BtrI	CACGTC	1 cut(s)	1006
BtsCI	GGATG	1 cut(s)	1116
BtsI	GCAGTG	1 cut(s)	535
BtsIMutI	CAGTG	2 cut(s)	535, 1600
Cac8I	GCNNGC	7 cut(s)	735, 758, 946, 970, 1323, 1356, 1583
CaiI	CAGNNNCTG	1 cut(s)	962
CciI	TCATGA	1 cut(s)	480
CfoI	GCGC	6 cut(s)	260, 542, 557, 1207, 1218, 1354
Cfr10I	RCCGGY	4 cut(s)	283, 332, 421, 968
Cfr13I	GGNCC	3 cut(s)	298, 995, 1370
ClaI	ATCGAT	1 cut(s)	453
Csp6I	GTAC	4 cut(s)	126, 883, 1226, 1546
CspAI	ACCGGT	1 cut(s)	332
CspCI	CAANNNNNGTGG	4 cut(s)	267, 302, 1362, 1397
CviAII	CATG	4 cut(s)	481, 606, 1045, 1284
CviQI	GTAC	4 cut(s)	126, 883, 1226, 1546
DdeI	CTNAG	1 cut(s)	94
DinI	GGCGCC	1 cut(s)	259
DpnI	GATC	9 cut(s)	441, 504, 564, 582, 750, 954, 960, 1161, 1508
DpnII	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
DrdI	GACNNNNNNGTC	1 cut(s)	377
DseDI	GACNNNNNNGTC	1 cut(s)	377
Eam1104I	CTCTTC	5 cut(s)	67, 746, 926, 1381, 1448
EarI	CTCTTC	5 cut(s)	67, 746, 926, 1381, 1448
Ecl136II	GAGCTC	3 cut(s)	478, 589, 775
Eco105I	TACGTA	1 cut(s)	125
Eco130I	CCWWGG	4 cut(s)	107, 465, 1283, 1335
Eco147I	AGGCCT	2 cut(s)	234, 446
Eco24I	GRGCYC	4 cut(s)	228, 480, 591, 777
Eco31I	GGTCTC	1 cut(s)	1065
Eco47I	GGWCC	2 cut(s)	298, 1370
Eco53kI	GAGCTC	3 cut(s)	478, 589, 775
Eco88I	CYCGRG	1 cut(s)	584
EcoICRI	GAGCTC	3 cut(s)	478, 589, 775
EcoRI	GAATTC	1 cut(s)	654
EcoRII	CCWGG	1 cut(s)	961
EcoT14I	CCWWGG	4 cut(s)	107, 465, 1283, 1335
EcoT38I	GRGCYC	4 cut(s)	228, 480, 591, 777
EgeI	GGCGCC	1 cut(s)	259
EheI	GGCGCC	1 cut(s)	259
ErhI	CCWWGG	4 cut(s)	107, 465, 1283, 1335
FaeI	CATG	4 cut(s)	484, 609, 1048, 1287
FaqI	GGGAC	2 cut(s)	171, 722
FatI	CATG	4 cut(s)	480, 605, 1044, 1283
FbaI	TGATCA	2 cut(s)	439, 562
FblI	GTMKAC	1 cut(s)	381
FokI	GGATG	1 cut(s)	1123
FriOI	GRGCYC	4 cut(s)	228, 480, 591, 777
FspBI	CTAG	6 cut(s)	23, 108, 168, 200, 815, 1068
GlaI	GCGC	6 cut(s)	259, 541, 556, 1206, 1217, 1353
GsaI	CCCAGC	1 cut(s)	1030
GsuI	CTGGAG	1 cut(s)	984
HaeII	RGCGCY	1 cut(s)	261
HaeIII	GGCC	6 cut(s)	234, 446, 470, 997, 1340, 1441
HhaI	GCGC	6 cut(s)	260, 542, 557, 1207, 1218, 1354
Hin1I	GRCGYC	2 cut(s)	258, 1292
Hin1II	CATG	4 cut(s)	484, 609, 1048, 1287
Hin6I	GCGC	6 cut(s)	258, 540, 555, 1205, 1216, 1352
HinP1I	GCGC	6 cut(s)	258, 540, 555, 1205, 1216, 1352
HincII	GTYRAC	2 cut(s)	382, 1290
HindII	GTYRAC	2 cut(s)	382, 1290
HindIII	AAGCTT	3 cut(s)	933, 1250, 1319
HinfI	GANTC	4 cut(s)	497, 710, 1241, 1412
HphI	GGTGA	5 cut(s)	6, 19, 379, 1028, 1543
Hpy166II	GTNNAC	6 cut(s)	382, 1264, 1276, 1290, 1546, 1618
Hpy188I	TCNGA	6 cut(s)	230, 370, 419, 502, 662, 779
Hpy188III	TCNNGA	9 cut(s)	481, 578, 584, 956, 979, 1009, 1163, 1368, 1615
Hpy8I	GTNNAC	6 cut(s)	382, 1264, 1276, 1290, 1546, 1618
HpyAV	CCTTC	6 cut(s)	67, 631, 1216, 1459, 1465, 1496
HpyCH4III	ACNGT	5 cut(s)	155, 242, 548, 1429, 1595
HpyCH4IV	ACGT	6 cut(s)	124, 184, 1005, 1292, 1548, 1620
HpyCH4V	TGCA	3 cut(s)	141, 278, 1358
HpyF3I	CTNAG	1 cut(s)	94
HpySE526I	ACGT	6 cut(s)	124, 184, 1005, 1292, 1548, 1620
Hsp92I	GRCGYC	2 cut(s)	258, 1292
Hsp92II	CATG	4 cut(s)	484, 609, 1048, 1287
HspAI	GCGC	6 cut(s)	258, 540, 555, 1205, 1216, 1352
KasI	GGCGCC	1 cut(s)	257
Kpn2I	TCCGGA	1 cut(s)	1367
KroI	GCCGGC	1 cut(s)	968
KroNI	GCCGGC	1 cut(s)	970
Ksp22I	TGATCA	2 cut(s)	439, 562
Kzo9I	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
LguI	GCTCTTC	1 cut(s)	926
LmnI	GCTCC	7 cut(s)	295, 314, 594, 780, 810, 965, 1608
Lsp1109I	GCAGC	9 cut(s)	329, 341, 437, 522, 717, 1051, 1219, 1343, 1370
LweI	GCATC	1 cut(s)	423
MaeI	CTAG	6 cut(s)	23, 108, 168, 200, 815, 1068
MaeII	ACGT	6 cut(s)	124, 184, 1005, 1292, 1548, 1620
MaeIII	GTNAC	3 cut(s)	25, 548, 1432
MalI	GATC	9 cut(s)	441, 504, 564, 582, 750, 954, 960, 1161, 1508
MbiI	CCGCTC	1 cut(s)	763
MboI	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
MflI	RGATCY	1 cut(s)	958
MhlI	GDGCHC	7 cut(s)	219, 228, 340, 480, 591, 777, 1626
MluCI	AATT	6 cut(s)	52, 87, 349, 654, 868, 1033
Mly113I	GGCGCC	1 cut(s)	258
MlyI	GAGTC	4 cut(s)	491, 704, 1235, 1406
MmeI	TCCRAC	4 cut(s)	824, 924, 1152, 1389
MroI	TCCGGA	1 cut(s)	1367
MroNI	GCCGGC	1 cut(s)	968
MseI	TTAA	2 cut(s)	119, 1551
MslI	CAYNNNNRTG	2 cut(s)	1098, 1346
MspA1I	CMGCKG	1 cut(s)	916
MspR9I	CCNGG	5 cut(s)	130, 720, 963, 1578, 1606
MvaI	CCWGG	1 cut(s)	963
MvnI	CGCG	6 cut(s)	557, 1205, 1218, 1230, 1309, 1354
NaeI	GCCGGC	1 cut(s)	970
NarI	GGCGCC	1 cut(s)	258
NciI	CCSGG	4 cut(s)	130, 720, 1578, 1606
NcoI	CCATGG	1 cut(s)	1283
NdeII	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
NgoMIV	GCCGGC	1 cut(s)	968
NlaIII	CATG	4 cut(s)	484, 609, 1048, 1287
NlaIV	GGNNCC	6 cut(s)	259, 291, 824, 949, 967, 1184
NmuCI	GTSAC	2 cut(s)	25, 548
PaeR7I	CTCGAG	1 cut(s)	584
PagI	TCATGA	1 cut(s)	480
PceI	AGGCCT	2 cut(s)	234, 446
PciSI	GCTCTTC	1 cut(s)	926
PcsI	WCGNNNNNNNCGW	1 cut(s)	1322
PdiI	GCCGGC	1 cut(s)	970
PflMI	CCANNNNNTGG	2 cut(s)	962, 1273
PfoI	TCCNGGA	2 cut(s)	718, 961
PinAI	ACCGGT	1 cut(s)	332
PleI	GAGTC	4 cut(s)	491, 704, 1235, 1406
PluTI	GGCGCC	1 cut(s)	261
PpsI	GAGTC	4 cut(s)	491, 704, 1235, 1406
Ppu21I	YACGTR	1 cut(s)	125
Psp124BI	GAGCTC	3 cut(s)	480, 591, 777
Psp6I	CCWGG	1 cut(s)	961
PspFI	CCCAGC	1 cut(s)	1026
PspGI	CCWGG	1 cut(s)	961
PspN4I	GGNNCC	6 cut(s)	259, 291, 824, 949, 967, 1184
PspPI	GGNCC	3 cut(s)	298, 995, 1370
PstNI	CAGNNNCTG	1 cut(s)	962
PsuI	RGATCY	1 cut(s)	958
RsaI	GTAC	4 cut(s)	127, 884, 1227, 1547
RsaNI	GTAC	4 cut(s)	126, 883, 1226, 1546
RseI	CAYNNNNRTG	2 cut(s)	1098, 1346
SacI	GAGCTC	3 cut(s)	480, 591, 777
SalI	GTCGAC	1 cut(s)	380
SapI	GCTCTTC	1 cut(s)	926
SaqAI	TTAA	2 cut(s)	119, 1551
Sau3AI	GATC	9 cut(s)	439, 502, 562, 580, 748, 952, 958, 1159, 1506
Sau96I	GGNCC	3 cut(s)	298, 995, 1370
SchI	GAGTC	4 cut(s)	491, 704, 1235, 1406
ScrFI	CCNGG	5 cut(s)	130, 720, 963, 1578, 1606
SduI	GDGCHC	7 cut(s)	219, 228, 340, 480, 591, 777, 1626
SfaNI	GCATC	1 cut(s)	423
SfoI	GGCGCC	1 cut(s)	259
Sfr274I	CTCGAG	1 cut(s)	584
SinI	GGWCC	2 cut(s)	298, 1370
SlaI	CTCGAG	1 cut(s)	584
SmiMI	CAYNNNNRTG	2 cut(s)	1098, 1346
SmlI	CTYRAG	1 cut(s)	584
SmoI	CTYRAG	1 cut(s)	584
SnaBI	TACGTA	1 cut(s)	125
SpeI	ACTAGT	1 cut(s)	22
Sse9I	AATT	6 cut(s)	52, 87, 349, 654, 868, 1033
SseBI	AGGCCT	2 cut(s)	234, 446
SsiI	CCGC	6 cut(s)	312, 412, 761, 916, 1307, 1529
SspDI	GGCGCC	1 cut(s)	257
SspMI	CTAG	6 cut(s)	23, 108, 168, 200, 815, 1068
SstI	GAGCTC	3 cut(s)	480, 591, 777
StuI	AGGCCT	2 cut(s)	234, 446
StyD4I	CCNGG	5 cut(s)	128, 718, 961, 1576, 1604
StyI	CCWWGG	4 cut(s)	107, 465, 1283, 1335
TaaI	ACNGT	5 cut(s)	155, 242, 548, 1429, 1595
TaiI	ACGT	6 cut(s)	127, 187, 1008, 1295, 1551, 1623
TaqI	TCGA	8 cut(s)	381, 453, 585, 978, 1104, 1316, 1415, 1509
TaqII	GACCGA	1 cut(s)	845
TasI	AATT	6 cut(s)	52, 87, 349, 654, 868, 1033
TatI	WGTACW	1 cut(s)	882
TauI	GCSGC	2 cut(s)	763, 919
Tru1I	TTAA	2 cut(s)	119, 1551
Tru9I	TTAA	2 cut(s)	119, 1551
TscAI	CASTG	2 cut(s)	542, 1600
TseFI	GTSAC	2 cut(s)	25, 548
TseI	GCWGC	9 cut(s)	317, 354, 425, 510, 730, 1064, 1207, 1331, 1358
Tsp45I	GTSAC	2 cut(s)	25, 548
TspDTI	ATGAA	3 cut(s)	189, 497, 740
TspRI	CASTG	2 cut(s)	542, 1600
Van91I	CCANNNNNTGG	2 cut(s)	962, 1273
VpaK11BI	GGWCC	2 cut(s)	298, 1370
XapI	RAATTY	3 cut(s)	52, 87, 654
XcmI	CCANNNNNNNNNTGG	3 cut(s)	702, 1005, 1010
XhoI	CTCGAG	1 cut(s)	584
XmaJI	CCTAGG	1 cut(s)	107
XmiI	GTMKAC	1 cut(s)	381
XspI	CTAG	6 cut(s)	23, 108, 168, 200, 815, 1068
ZraI	GACGTC	1 cut(s)	1293

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.