Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Forward (+)

5547864 .. 5549681

Gene Structure 1818 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G005340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGGCTTCTCTGTCACCTCCACTAGTCACCACCTCAAGTACCATTCCCAGTACTACAACTTCTCTGTCTTCCTTCTGTCAAAAGAATTCCCAAATTTCCTTAATTGGAAAACCCAGGCATTCCTATGCACGCAGTAGAGTCTCATGCAAAGCCACAAACAGTGACCAAAAGCCGTCCAACAACTCCGACGATGCAAAACCTTTGGGCAAAACCGTAGACAGGAGAAATGTGCTTCTCGGTCTCGGAGGTCTGTATGGCGTGGCTGGTCTTGGCACTGACCCTTTTGCCTTTGCCAAGCCTGTGGCTCCTCCTGATGTCTCCAAGTGTGGGGCCGCAGACTTGCCTACAGGTGCACAACCAACTGATTGTTGCCCACCGATAGCCAAAAAGATAATCGATTTCCAGCTCCCACCACAGTATAAACTCCGCGTCAGGCCAGCGGCTCACGCCGTTGACAAAGCCTACATTGAGAAATACTCCAAGGCCATCGAGCTCATGAAAGCTCTCCCCGATGACGATCCACGTAGCTTCAAGCAACAAGCCAACGTTCACTGTGCTTATTGTGACGGAGCTTACGACCAAGCTGGCTTTCCCGATCTCGAGCTCCAAATCCACGGCTCATGGCTATTCTTTCCCTTCCACCGATACTACTTGTACTTCTACGAGAGAATCCTAGGCAAGCTCATCAATGACCCCTCGTTTGCCTTGCCTTTCTGGAACTGGGATGCTCCGTCGCCGCTCTATGACAAGTTCCGAAATGCCAACCACCAGCCGCCGAGGCTCATTGACCTCAACTACAATCTTAAGGATGAAAACGTCTCCAACGAAACTCAAATCAACACCAACCTCAAGATCATGTACAGGCAAATGGTGTCCAACGCCAAGAACCCTAAGCTGTTCTTTGGAAGCCCTTACAGGGCAGGGGACGAGCCTAGCCCCGGAGGCGGATCAATAGAGACTACTCCACATGGACCGGTCCACATTTGGACCGGCGACAACACCCAGCCGAATCTTGAGGACATGGGAAACTTCTACTCTGCTGGTAGAGACCCTATATTTTTCTCTCACCACTCTAACGTTGACCGTTTGTGGAGCCTGTGGAAGACCCTAGGAGGCAAAAGAGCGGATTTCACAGATTCTGATTGGTTGGACTCCGGGTTTTTGTTCTATGATGAGAACGCGAATATGGTGCGCGTGAAGGTACGTGACTGCCTCGATTCCAAGAAGCTAGGATATGTGTACCAAGACGTAGACATCCCATGGGTAAGCCACAAGCCGACTCCTCGCCGTGTCAAGGATGCCATAAAGAAATTAGGTCACAAGCTCCATGGCGTGGCTCACGCCGCGGAGACTTCCACCAAGGTAGTGGCAGGGGCTCATTTTCCTATAAGCTTGGCTTCAAAGATTAGTATTGCAGTGGCCAGGCCACAACAGAAGAAGAGGAGCCAGAAGGAGAAGGACCACGAGGAGGAGGTATTGGTGATTGAAGGGATTGAGTTTGACAGAGACGTGGGGGTGAAGTTTGATGTGTATGTGAACGATGAGGATGACTTGCCGAGTGGACCGGAGAAGAGTGAGTTTGCTGGGAGCTTTGTGAGTGTGCCGCACAGGCATAAGCATACCAAGAAGATTAACACTATCTTGAGGTTAGGTTTGACAGATTTGCTGGAGGATTTGGAAGCCGAGGACGATGAGACTGTCGTGGTGACTTTGATTCCAAGGTATGGGGCTGAGAAAGTCAAGATTGGCGGCATCAAGATTGAGTTTGCTTCAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

592

Amino Acids

66.03

Weight (kDa)

7.03

Isoelectric Point (pI)

40.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	175 - 252	3.9e-17	Common central domain of tyrosinase
Tyrosinase	PF00264	265 - 369	1.1e-16	Common central domain of tyrosinase
PPO1_DWL	PF12142	376 - 427	1.9e-27	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	461 - 589	1.7e-53	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	1333, 1724
AccBSI	CCGCTC	2 cut(s)	739, 1124
AccI	GTMKAC	2 cut(s)	216, 1251
AccII	CGCG	4 cut(s)	429, 1181, 1194, 1346
AclI	AACGTT	2 cut(s)	546, 1077
AclWI	GGATC	2 cut(s)	512, 955
AcoI	YGGCCR	1 cut(s)	1419
AcsI	RAATTY	2 cut(s)	85, 93
AcuI	CTGAAG	1 cut(s)	1755
AfaI	GTAC	6 cut(s)	40, 52, 656, 860, 1203, 1241
AfiI	CCNNNNNNNGG	9 cut(s)	219, 327, 916, 938, 944, 1294, 1333, 1468, 1724
AflII	CTTAAG	1 cut(s)	803
AgeI	ACCGGT	1 cut(s)	973
AgsI	TTSAA	3 cut(s)	532, 1401, 1487
AhlI	ACTAGT	1 cut(s)	22
AjiI	CACGTC	1 cut(s)	1510
AjnI	CCWGG	2 cut(s)	113, 1421
AjuI	GAANNNNNNNTTGG	2 cut(s)	573, 605
Alw21I	GWGCWC	3 cut(s)	355, 495, 606
Alw26I	GTCTC	9 cut(s)	145, 245, 322, 823, 950, 1041, 1343, 1500, 1688
Alw44I	GTGCAC	1 cut(s)	351
AlwI	GGATC	2 cut(s)	512, 955
AlwNI	CAGNNNCTG	1 cut(s)	1139
Ama87I	CYCGRG	1 cut(s)	599
AoxI	GGCC	5 cut(s)	330, 434, 483, 1419, 1424
ApaLI	GTGCAC	1 cut(s)	351
ApoI	RAATTY	2 cut(s)	85, 93
ArsI	GACNNNNNNTTYG	2 cut(s)	683, 715
AsiGI	ACCGGT	1 cut(s)	973
AspA2I	CCTAGG	2 cut(s)	673, 1108
AspLEI	GCGC	1 cut(s)	1194
AspS9I	GGNCC	6 cut(s)	330, 971, 976, 987, 1459, 1562
AsuC2I	CCSGG	2 cut(s)	939, 1156
AsuHPI	GGTGA	6 cut(s)	6, 19, 1058, 1492, 1528, 1717
AvaI	CYCGRG	1 cut(s)	599
AvaII	GGWCC	5 cut(s)	971, 976, 987, 1459, 1562
AvrII	CCTAGG	2 cut(s)	673, 1108
BaeGI	GKGCMC	1 cut(s)	355
BalI	TGGCCA	1 cut(s)	1421
BanII	GRGCYC	3 cut(s)	495, 606, 1378
BarI	GAAGNNNNNNTAC	2 cut(s)	43, 75
BauI	CACGAG	1 cut(s)	1463
BbsI	GAAGAC	2 cut(s)	60, 1109
Bbv12I	GWGCWC	3 cut(s)	355, 495, 606
BccI	CCATC	1 cut(s)	494
BceAI	ACGGC	4 cut(s)	157, 434, 631, 1272
BcgI	CGANNNNNNTGC	2 cut(s)	1171, 1205
BciT130I	CCWGG	2 cut(s)	115, 1423
BcnI	CCSGG	2 cut(s)	939, 1156
BcoDI	GTCTC	9 cut(s)	145, 245, 322, 823, 950, 1041, 1343, 1500, 1688
BcuI	ACTAGT	1 cut(s)	22
BfaI	CTAG	5 cut(s)	23, 674, 933, 1109, 1229
BfmI	CTRYAG	1 cut(s)	345
BfrI	CTTAAG	1 cut(s)	803
BglI	GCCNNNNNGGC	3 cut(s)	778, 942, 1609
BisI	GCNGC	7 cut(s)	333, 441, 737, 773, 1344, 1604, 1750
BlnI	CCTAGG	2 cut(s)	673, 1108
BlsI	GCNGC	7 cut(s)	334, 442, 738, 774, 1345, 1605, 1751
BmcAI	AGTACT	1 cut(s)	52
Bme1390I	CCNGG	4 cut(s)	115, 939, 1156, 1423
Bme18I	GGWCC	5 cut(s)	971, 976, 987, 1459, 1562
BmeT110I	CYCGRG	1 cut(s)	599
BmgBI	CACGTC	1 cut(s)	1510
BmgT120I	GGNCC	6 cut(s)	330, 971, 976, 987, 1459, 1562
BmiI	GGNNCC	4 cut(s)	306, 331, 1094, 1445
BmrFI	CCNGG	4 cut(s)	115, 939, 1156, 1423
BmrI	ACTGGG	2 cut(s)	42, 730
BmsI	GCATC	4 cut(s)	181, 715, 1288, 1761
BmuI	ACTGGG	2 cut(s)	42, 730
BpiI	GAAGAC	2 cut(s)	60, 1109
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
BpmI	CTGGAG	1 cut(s)	1688
Bpu10I	CCTNAGC	1 cut(s)	891
BpuEI	CTTGAG	4 cut(s)	19, 833, 1034, 1663
BpuMI	CCSGG	2 cut(s)	939, 1156
Bsa29I	ATCGAT	1 cut(s)	396
BsaAI	YACGTR	2 cut(s)	524, 1205
BsaBI	GATNNNNATC	1 cut(s)	516
BsaI	GGTCTC	2 cut(s)	245, 1041
BsaWI	WCCGGW	2 cut(s)	973, 1564
Bsc4I	CCNNNNNNNGG	9 cut(s)	219, 327, 916, 938, 944, 1294, 1333, 1468, 1724
Bse118I	RCCGGY	2 cut(s)	973, 989
Bse1I	ACTGG	2 cut(s)	48, 725
Bse8I	GATNNNNATC	1 cut(s)	516
BseBI	CCWGG	2 cut(s)	115, 1423
BseCI	ATCGAT	1 cut(s)	396
BseGI	GGATG	5 cut(s)	730, 814, 1254, 1303, 1552
BseJI	GATNNNNATC	1 cut(s)	516
BseLI	CCNNNNNNNGG	9 cut(s)	219, 327, 916, 938, 944, 1294, 1333, 1468, 1724
BseMII	CTCAG	1 cut(s)	1722
BseNI	ACTGG	2 cut(s)	48, 725
BseRI	GAGGAG	5 cut(s)	297, 1272, 1456, 1481, 1484
BseSI	GKGCMC	1 cut(s)	355
BseYI	CCCAGC	2 cut(s)	1002, 1583
Bsh1236I	CGCG	4 cut(s)	429, 1181, 1194, 1346
BshFI	GGCC	5 cut(s)	332, 436, 485, 1421, 1426
BshTI	ACCGGT	1 cut(s)	973
BshVI	ATCGAT	1 cut(s)	396
BsiHKAI	GWGCWC	3 cut(s)	355, 495, 606
BsiHKCI	CYCGRG	1 cut(s)	599
BsiSI	CCGG	5 cut(s)	939, 974, 990, 1155, 1565
BslFI	GGGAC	1 cut(s)	938
BslI	CCNNNNNNNGG	9 cut(s)	219, 327, 916, 938, 944, 1294, 1333, 1468, 1724
BsmAI	GTCTC	9 cut(s)	145, 245, 322, 823, 950, 1041, 1343, 1500, 1688
BsmBI	CGTCTC	2 cut(s)	823, 1500
BsmFI	GGGAC	1 cut(s)	938
BsmI	GAATGC	1 cut(s)	118
BsnI	GGCC	5 cut(s)	332, 436, 485, 1421, 1426
Bso31I	GGTCTC	2 cut(s)	245, 1041
BsoBI	CYCGRG	1 cut(s)	599
Bsp1286I	GDGCHC	4 cut(s)	355, 495, 606, 1378
Bsp1407I	TGTACA	1 cut(s)	858
Bsp143I	GATC	4 cut(s)	517, 595, 852, 947
Bsp19I	CCATGG	2 cut(s)	1259, 1327
BspANI	GGCC	5 cut(s)	332, 436, 485, 1421, 1426
BspCNI	CTCAG	1 cut(s)	1723
BspDI	ATCGAT	1 cut(s)	396
BspFNI	CGCG	4 cut(s)	429, 1181, 1194, 1346
BspHI	TCATGA	1 cut(s)	495
BspLI	GGNNCC	4 cut(s)	306, 331, 1094, 1445
BspPI	GGATC	2 cut(s)	512, 955
BspTI	CTTAAG	1 cut(s)	803
BspTNI	GGTCTC	2 cut(s)	245, 1041
BsrBI	CCGCTC	2 cut(s)	739, 1124
BsrFI	RCCGGY	2 cut(s)	973, 989
BsrGI	TGTACA	1 cut(s)	858
BsrI	ACTGG	2 cut(s)	48, 725
BssAI	RCCGGY	2 cut(s)	973, 989
BssMI	GATC	4 cut(s)	517, 595, 852, 947
BssSI	CACGAG	1 cut(s)	1463
BssT1I	CCWWGG	7 cut(s)	480, 673, 1108, 1259, 1327, 1359, 1718
Bst2BI	CACGAG	1 cut(s)	1463
Bst2UI	CCWGG	2 cut(s)	115, 1423
Bst4CI	ACNGT	6 cut(s)	161, 214, 417, 554, 1085, 1699
Bst6I	CTCTTC	2 cut(s)	1433, 1565
BstAFI	CTTAAG	1 cut(s)	803
BstAUI	TGTACA	1 cut(s)	858
BstBAI	YACGTR	2 cut(s)	524, 1205
BstC8I	GCNNGC	4 cut(s)	130, 438, 586, 680
BstDEI	CTNAG	2 cut(s)	891, 1731
BstDSI	CCRYGG	4 cut(s)	613, 1259, 1327, 1344
BstF5I	GGATG	5 cut(s)	730, 814, 1254, 1303, 1552
BstFNI	CGCG	4 cut(s)	429, 1181, 1194, 1346
BstHHI	GCGC	1 cut(s)	1194
BstKTI	GATC	4 cut(s)	520, 598, 855, 950
BstMAI	GTCTC	9 cut(s)	145, 245, 322, 823, 950, 1041, 1343, 1500, 1688
BstMBI	GATC	4 cut(s)	517, 595, 852, 947
BstMWI	GCNNNNNNNGC	5 cut(s)	446, 778, 942, 1343, 1609
BstNI	CCWGG	2 cut(s)	115, 1423
BstSCI	CCNGG	4 cut(s)	113, 937, 1154, 1421
BstSFI	CTRYAG	1 cut(s)	345
BstSLI	GKGCMC	1 cut(s)	355
BstUI	CGCG	4 cut(s)	429, 1181, 1194, 1346
BstV2I	GAAGAC	2 cut(s)	60, 1109
BstXI	CCANNNNNNTGG	2 cut(s)	301, 1366
Bsu15I	ATCGAT	1 cut(s)	396
BsuRI	GGCC	5 cut(s)	332, 436, 485, 1421, 1426
BsuTUI	ATCGAT	1 cut(s)	396
BtgI	CCRYGG	4 cut(s)	613, 1259, 1327, 1344
BtrI	CACGTC	1 cut(s)	1510
BtsCI	GGATG	5 cut(s)	730, 814, 1254, 1303, 1552
BtsI	GCAGTG	1 cut(s)	1422
BtsIMutI	CAGTG	4 cut(s)	166, 273, 550, 1422
Cac8I	GCNNGC	4 cut(s)	130, 438, 586, 680
CaiI	CAGNNNCTG	1 cut(s)	1139
CciI	TCATGA	1 cut(s)	495
CfoI	GCGC	1 cut(s)	1194
Cfr10I	RCCGGY	2 cut(s)	973, 989
Cfr13I	GGNCC	6 cut(s)	330, 971, 976, 987, 1459, 1562
Cfr42I	CCGCGG	1 cut(s)	1347
ClaI	ATCGAT	1 cut(s)	396
CseI	GACGC	1 cut(s)	418
Csp6I	GTAC	6 cut(s)	39, 51, 655, 859, 1202, 1240
CspAI	ACCGGT	1 cut(s)	973
CviAII	CATG	8 cut(s)	144, 496, 621, 856, 968, 1021, 1260, 1328
CviQI	GTAC	6 cut(s)	39, 51, 655, 859, 1202, 1240
DdeI	CTNAG	2 cut(s)	891, 1731
DpnI	GATC	4 cut(s)	519, 597, 854, 949
DpnII	GATC	4 cut(s)	517, 595, 852, 947
EaeI	YGGCCR	1 cut(s)	1419
Eam1104I	CTCTTC	2 cut(s)	1433, 1565
EarI	CTCTTC	2 cut(s)	1433, 1565
EciI	GGCGGA	1 cut(s)	960
Ecl136II	GAGCTC	2 cut(s)	493, 604
Eco130I	CCWWGG	7 cut(s)	480, 673, 1108, 1259, 1327, 1359, 1718
Eco24I	GRGCYC	3 cut(s)	495, 606, 1378
Eco31I	GGTCTC	2 cut(s)	245, 1041
Eco47I	GGWCC	5 cut(s)	971, 976, 987, 1459, 1562
Eco53kI	GAGCTC	2 cut(s)	493, 604
Eco57I	CTGAAG	1 cut(s)	1755
Eco88I	CYCGRG	1 cut(s)	599
EcoICRI	GAGCTC	2 cut(s)	493, 604
EcoRI	GAATTC	1 cut(s)	85
EcoRII	CCWGG	2 cut(s)	113, 1421
EcoT14I	CCWWGG	7 cut(s)	480, 673, 1108, 1259, 1327, 1359, 1718
EcoT38I	GRGCYC	3 cut(s)	495, 606, 1378
ErhI	CCWWGG	7 cut(s)	480, 673, 1108, 1259, 1327, 1359, 1718
Esp3I	CGTCTC	2 cut(s)	823, 1500
FaeI	CATG	8 cut(s)	147, 499, 624, 859, 971, 1024, 1263, 1331
FalI	AAGNNNNNCTT	4 cut(s)	884, 916, 1381, 1413
FaqI	GGGAC	1 cut(s)	938
FatI	CATG	8 cut(s)	143, 495, 620, 855, 967, 1020, 1259, 1327
FblI	GTMKAC	2 cut(s)	216, 1251
Fnu4HI	GCNGC	7 cut(s)	333, 441, 737, 773, 1344, 1604, 1750
FokI	GGATG	5 cut(s)	737, 821, 1241, 1310, 1559
FriOI	GRGCYC	3 cut(s)	495, 606, 1378
Fsp4HI	GCNGC	7 cut(s)	333, 441, 737, 773, 1344, 1604, 1750
FspBI	CTAG	5 cut(s)	23, 674, 933, 1109, 1229
GlaI	GCGC	1 cut(s)	1193
GluI	GCNGC	7 cut(s)	333, 441, 737, 773, 1344, 1604, 1750
GsaI	CCCAGC	2 cut(s)	1006, 1587
GsuI	CTGGAG	1 cut(s)	1688
HaeIII	GGCC	5 cut(s)	332, 436, 485, 1421, 1426
HapII	CCGG	5 cut(s)	939, 974, 990, 1155, 1565
HgaI	GACGC	1 cut(s)	418
HhaI	GCGC	1 cut(s)	1194
Hin1II	CATG	8 cut(s)	147, 499, 624, 859, 971, 1024, 1263, 1331
Hin6I	GCGC	1 cut(s)	1192
HinP1I	GCGC	1 cut(s)	1192
HincII	GTYRAC	2 cut(s)	454, 1081
HindII	GTYRAC	2 cut(s)	454, 1081
HindIII	AAGCTT	1 cut(s)	1390
HinfI	GANTC	8 cut(s)	138, 669, 1009, 1136, 1151, 1217, 1279, 1714
HpaII	CCGG	5 cut(s)	939, 974, 990, 1155, 1565
HphI	GGTGA	6 cut(s)	6, 19, 1058, 1492, 1528, 1717
Hpy188I	TCNGA	5 cut(s)	187, 245, 755, 1141, 1774
Hpy99I	CGWCG	2 cut(s)	191, 736
HpyAV	CCTTC	6 cut(s)	82, 646, 1192, 1444, 1450, 1481
HpyCH4III	ACNGT	6 cut(s)	161, 214, 417, 554, 1085, 1699
HpyCH4IV	ACGT	7 cut(s)	523, 546, 816, 1077, 1204, 1248, 1509
HpyCH4V	TGCA	5 cut(s)	128, 147, 194, 353, 1415
HpyF10VI	GCNNNNNNNGC	5 cut(s)	446, 778, 942, 1343, 1609
HpyF3I	CTNAG	2 cut(s)	891, 1731
HpySE526I	ACGT	7 cut(s)	523, 546, 816, 1077, 1204, 1248, 1509
Hsp92II	CATG	8 cut(s)	147, 499, 624, 859, 971, 1024, 1263, 1331
HspAI	GCGC	1 cut(s)	1192
KspI	CCGCGG	1 cut(s)	1347
Kzo9I	GATC	4 cut(s)	517, 595, 852, 947
LmnI	GCTCC	9 cut(s)	310, 411, 569, 609, 733, 1092, 1329, 1443, 1587
LweI	GCATC	4 cut(s)	181, 715, 1288, 1761
MaeI	CTAG	5 cut(s)	23, 674, 933, 1109, 1229
MaeII	ACGT	7 cut(s)	523, 546, 816, 1077, 1204, 1248, 1509
MaeIII	GTNAC	7 cut(s)	12, 25, 161, 563, 1205, 1316, 1705
MalI	GATC	4 cut(s)	519, 597, 854, 949
MbiI	CCGCTC	2 cut(s)	739, 1124
MboI	GATC	4 cut(s)	517, 595, 852, 947
MboII	GAAGA	6 cut(s)	60, 1114, 1447, 1450, 1582, 1639
MhlI	GDGCHC	4 cut(s)	355, 495, 606, 1378
MlsI	TGGCCA	1 cut(s)	1421
MluCI	AATT	4 cut(s)	85, 93, 102, 1310
MluNI	TGGCCA	1 cut(s)	1421
MlyI	GAGTC	3 cut(s)	147, 1145, 1273
MmeI	TCCRAC	5 cut(s)	201, 210, 846, 900, 1128
Mox20I	TGGCCA	1 cut(s)	1421
MscI	TGGCCA	1 cut(s)	1421
MseI	TTAA	4 cut(s)	101, 804, 1632, 1777
MslI	CAYNNNNRTG	1 cut(s)	123
Msp20I	TGGCCA	1 cut(s)	1421
MspA1I	CMGCKG	2 cut(s)	440, 1346
MspCI	CTTAAG	1 cut(s)	803
MspI	CCGG	5 cut(s)	939, 974, 990, 1155, 1565
MspR9I	CCNGG	4 cut(s)	115, 939, 1156, 1423
Mva1269I	GAATGC	1 cut(s)	118
MvaI	CCWGG	2 cut(s)	115, 1423
MvnI	CGCG	4 cut(s)	429, 1181, 1194, 1346
MwoI	GCNNNNNNNGC	5 cut(s)	446, 778, 942, 1343, 1609
NciI	CCSGG	2 cut(s)	939, 1156
NcoI	CCATGG	2 cut(s)	1259, 1327
NdeII	GATC	4 cut(s)	517, 595, 852, 947
NlaIII	CATG	8 cut(s)	147, 499, 624, 859, 971, 1024, 1263, 1331
NlaIV	GGNNCC	4 cut(s)	306, 331, 1094, 1445
NmeAIII	GCCGAG	3 cut(s)	801, 1581, 1708
NmuCI	GTSAC	7 cut(s)	12, 25, 161, 563, 1205, 1316, 1705
PaeR7I	CTCGAG	1 cut(s)	599
PagI	TCATGA	1 cut(s)	495
PcsI	WCGNNNNNNNCGW	2 cut(s)	573, 822
PctI	GAATGC	1 cut(s)	118
PfeI	GAWTC	5 cut(s)	669, 1009, 1136, 1217, 1714
PflMI	CCANNNNNTGG	2 cut(s)	1333, 1724
PinAI	ACCGGT	1 cut(s)	973
PkrI	GCNGC	7 cut(s)	334, 442, 738, 774, 1345, 1605, 1751
PleI	GAGTC	3 cut(s)	146, 1145, 1273
PpsI	GAGTC	3 cut(s)	146, 1145, 1273
Ppu21I	YACGTR	2 cut(s)	524, 1205
Psp124BI	GAGCTC	2 cut(s)	495, 606
Psp1406I	AACGTT	2 cut(s)	546, 1077
Psp6I	CCWGG	2 cut(s)	113, 1421
PspFI	CCCAGC	2 cut(s)	1002, 1583
PspGI	CCWGG	2 cut(s)	113, 1421
PspN4I	GGNNCC	4 cut(s)	306, 331, 1094, 1445
PspPI	GGNCC	6 cut(s)	330, 971, 976, 987, 1459, 1562
PstNI	CAGNNNCTG	1 cut(s)	1139
RsaI	GTAC	6 cut(s)	40, 52, 656, 860, 1203, 1241
RsaNI	GTAC	6 cut(s)	39, 51, 655, 859, 1202, 1240
RseI	CAYNNNNRTG	1 cut(s)	123
SacI	GAGCTC	2 cut(s)	495, 606
SacII	CCGCGG	1 cut(s)	1347
SaqAI	TTAA	4 cut(s)	101, 804, 1632, 1777
SatI	GCNGC	7 cut(s)	333, 441, 737, 773, 1344, 1604, 1750
Sau3AI	GATC	4 cut(s)	517, 595, 852, 947
Sau96I	GGNCC	6 cut(s)	330, 971, 976, 987, 1459, 1562
ScaI	AGTACT	1 cut(s)	52
SchI	GAGTC	3 cut(s)	147, 1145, 1273
ScrFI	CCNGG	4 cut(s)	115, 939, 1156, 1423
SduI	GDGCHC	4 cut(s)	355, 495, 606, 1378
SfaNI	GCATC	4 cut(s)	181, 715, 1288, 1761
SfcI	CTRYAG	1 cut(s)	345
Sfr274I	CTCGAG	1 cut(s)	599
Sfr303I	CCGCGG	1 cut(s)	1347
SgrBI	CCGCGG	1 cut(s)	1347
SinI	GGWCC	5 cut(s)	971, 976, 987, 1459, 1562
SlaI	CTCGAG	1 cut(s)	599
SmiMI	CAYNNNNRTG	1 cut(s)	123
SmlI	CTYRAG	6 cut(s)	34, 599, 803, 848, 1013, 1642
SmoI	CTYRAG	6 cut(s)	34, 599, 803, 848, 1013, 1642
SpeI	ACTAGT	1 cut(s)	22
Sse9I	AATT	4 cut(s)	85, 93, 102, 1310
SspMI	CTAG	5 cut(s)	23, 674, 933, 1109, 1229
SstI	GAGCTC	2 cut(s)	495, 606
StyD4I	CCNGG	4 cut(s)	113, 937, 1154, 1421
StyI	CCWWGG	7 cut(s)	480, 673, 1108, 1259, 1327, 1359, 1718
TaaI	ACNGT	6 cut(s)	161, 214, 417, 554, 1085, 1699
TaiI	ACGT	7 cut(s)	526, 549, 819, 1080, 1207, 1251, 1512
TaqI	TCGA	4 cut(s)	396, 489, 600, 1215
TaqII	GACCGA	1 cut(s)	227
TasI	AATT	4 cut(s)	85, 93, 102, 1310
TatI	WGTACW	3 cut(s)	50, 654, 858
TauI	GCSGC	7 cut(s)	335, 443, 739, 775, 1346, 1606, 1752
TfiI	GAWTC	5 cut(s)	669, 1009, 1136, 1217, 1714
Tru1I	TTAA	4 cut(s)	101, 804, 1632, 1777
Tru9I	TTAA	4 cut(s)	101, 804, 1632, 1777
TscAI	CASTG	4 cut(s)	166, 280, 557, 1422
TseFI	GTSAC	7 cut(s)	12, 25, 161, 563, 1205, 1316, 1705
Tsp45I	GTSAC	7 cut(s)	12, 25, 161, 563, 1205, 1316, 1705
TspDTI	ATGAA	2 cut(s)	512, 825
TspGWI	ACGGA	2 cut(s)	582, 720
TspRI	CASTG	4 cut(s)	166, 280, 557, 1422
Van91I	CCANNNNNTGG	2 cut(s)	1333, 1724
Vha464I	CTTAAG	1 cut(s)	803
VneI	GTGCAC	1 cut(s)	351
VpaK11BI	GGWCC	5 cut(s)	971, 976, 987, 1459, 1562
XapI	RAATTY	2 cut(s)	85, 93
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1363
XhoI	CTCGAG	1 cut(s)	599
XmaJI	CCTAGG	2 cut(s)	673, 1108
XmiI	GTMKAC	2 cut(s)	216, 1251
XspI	CTAG	5 cut(s)	23, 674, 933, 1109, 1229
ZrmI	AGTACT	1 cut(s)	52

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.