Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Reverse (-)

38661973 .. 38663805

Gene Structure 1833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1299300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGGCTTCTATGTCAGCTCCACTCGTCACCCCCACCACAAGTATCATCCCCACAACTTCTCTCTCTCCTTTCTCCCAAAAGTATCACCGAATATCCTCATTTGGAAACCCTAGGCATTCCAATTTACAAGCTGTCTCATGCAAAGCCACAAATAATAGTAGTGACCAAAACAAAAACCCTTCCACTAGCTCCAACGATCACGACCATGAAAACCCTTCTCCAGTAAACCTAGACAGAAGACATGTACTTATAGGTCTCGGAAGCCTGTACGGTGGAGTGGCTGGTCTTGGCAGCGACCCCTTCGCTGTTGCAAAGCCAGTGTCGCCGCCTGACCTAGCCAAATGCGGAGCTGCGGACTTTCCAAGTGGAGCAGTCCCGACCAACTGTTGCCCGCCAACGTCCCAAAAAATCGTAGACTTCAAATTCCCCTCCCCTACCAAACTCCGCGTCAGGCCGGCAGCTCACGCCGTGGATAAAGCCTACATCGAAAAATACTCAAAAGCCATCGAGCTCATGAAAGCCCTCCCGGACGACGATCCGCGTAGCTTCACCCAGCAAGCCGATGTCCACTGTGCCTATTGTAACGGTGCGTACGACCAAGTCGGCTTCCCCAACCTCGAGCTCCAAATCCATCAATCCTGGCTTTTCTTCCCCTTCCATCGTTACTACCTATATTTCCACGAAAGAATCTTGGCCAAACTCATAGACGATCCGACCTTCGCGTTGCCGTTTTGGAACTGGGACGCGCCAGCTGGCATGCAACTCCCTGCCTTGTACGCTAACCCTGACTCTCCGCTTTACGACGAGCTCCGCGCTGCCAGCCATCAGCCGCCAACTCTCATCGATCTTGACTTCAACGGCACGGATGAAACAATGTCCAAGGATGCTCAAATCGAAGCCAACCTCAAAATCATGTATAGGCAGATGGTTTCCAACTCCAAGAAACCGCTGTTGTTCTTTGGTTCGCCCTACAGGGCTGGCACTGAACCAGATCCAGGGGCCGGTGCAATCGAACAGACCCCACATGGTCCGGTTCATACATGGACCGGAGATAACACGCAACCTAATTATGAAGACATGGGGAATTTTTACTCCACTGCAAGGGATCCGATATTTTTTGCGCACCATTTGAATCTGGATCGAATGTGGAATATTTGGAAAAGTATAGGGACTAAAAATAAAGATATTAACGATAAGGATTGGTTGGATACGGGGTTTTTGTTTTATGACGAGAATGCTGAGCTTGTTAGGGTCACGGTGAGGGACACTCTTGATAATAAAAAGCTAGGGTATACGTATGAAGATGTTGAGATTCCATGGCTCAACTCTAGACCGACGCCACGTCGGACAAAGCTTGCGAGAAAGGCAAAGGCGGCTGGAGTGGCGAAGGCCGCTGAGACGACGTCATCGGGGAAGGTGGTGGCGGGTAAAGATTTTCCAATAAATTTGGAGACGAAGATAACTACGGTGGTGTCAAGGCCGAAGCCGAAGAAGAGGAGCAAGAAGGAGAAAGAGGATGAGGAGGAGATATTGGTGATTCGGGGGATTGAGCTTGACAAAGATGTGGCTGTGAAGTTTGATGTGTATGTGAATGACGTGGATGATGAGGATGCGGCACCGAGTGGACCCGACAAGAGCGAGTTTGCTGGGAGTTTTGTGAGTGTGCCACATAAGCAGAAGGAAAAGAGCAAGAGTTGTTTAAGGTTGGGGTTAACGGACCTGTTGGAGGATTTGGGTGCTGAAGATGATGAGAGTGTGGTGGTGACTTTAGTGCCCAGGTACGGCGCTCAGGCTGTTAAGATCGGTAGCATCAAAATTGAGTTTCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

611

Amino Acids

67.67

Weight (kDa)

5.93

Isoelectric Point (pI)

43.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	181 - 387	1.2e-34	Common central domain of tyrosinase
PPO1_DWL	PF12142	395 - 446	2.6e-26	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	479 - 608	1.5e-50	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1406
Acc16I	TGCGCA	1 cut(s)	1122
AccB1I	GGYRCC	1 cut(s)	1615
AccI	GTMKAC	2 cut(s)	414, 1292
AccII	CGCG	5 cut(s)	447, 541, 722, 746, 813
AclWI	GGATC	6 cut(s)	530, 704, 986, 1100, 1113, 1146
AcoI	YGGCCR	1 cut(s)	693
AcsI	RAATTY	3 cut(s)	422, 1084, 1444
AcuI	CTGAAG	1 cut(s)	1761
AcyI	GRCGYC	2 cut(s)	1337, 1403
AdeI	CACNNNGTG	2 cut(s)	469, 1622
AfaI	GTAC	5 cut(s)	246, 269, 593, 776, 1781
AfiI	CCNNNNNNNGG	5 cut(s)	995, 1001, 1101, 1726, 1781
AflIII	ACRYGT	1 cut(s)	241
AgsI	TTSAA	3 cut(s)	421, 856, 1132
AhdI	GACNNNNNGTC	1 cut(s)	1341
AjiI	CACGTC	2 cut(s)	1343, 1597
AjnI	CCWGG	3 cut(s)	638, 994, 1775
Alw21I	GWGCWC	3 cut(s)	513, 624, 810
Alw26I	GTCTC	4 cut(s)	139, 260, 1391, 1445
AlwI	GGATC	6 cut(s)	530, 704, 986, 1100, 1113, 1146
Ama87I	CYCGRG	1 cut(s)	617
AoxI	GGCC	5 cut(s)	452, 693, 999, 1389, 1478
ApeKI	GCWGC	4 cut(s)	291, 350, 458, 815
ApoI	RAATTY	3 cut(s)	422, 1084, 1444
ArsI	GACNNNNNNTTYG	4 cut(s)	305, 337, 432, 464
AspA2I	CCTAGG	1 cut(s)	110
AspLEI	GCGC	4 cut(s)	748, 815, 1123, 1787
AspS9I	GGNCC	5 cut(s)	999, 1028, 1044, 1625, 1717
AsuC2I	CCSGG	1 cut(s)	527
AsuHPI	GGTGA	6 cut(s)	19, 77, 541, 1270, 1546, 1774
AvaI	CYCGRG	1 cut(s)	617
AvaII	GGWCC	4 cut(s)	1028, 1044, 1625, 1717
AvrII	CCTAGG	1 cut(s)	110
BaeGI	GKGCMC	1 cut(s)	1776
BalI	TGGCCA	1 cut(s)	695
BamHI	GGATCC	1 cut(s)	1105
BanI	GGYRCC	1 cut(s)	1615
BanII	GRGCYC	3 cut(s)	513, 624, 810
BbsI	GAAGAC	2 cut(s)	244, 1080
Bbv12I	GWGCWC	3 cut(s)	513, 624, 810
BbvI	GCAGC	4 cut(s)	303, 337, 470, 802
BccI	CCATC	5 cut(s)	512, 639, 666, 831, 919
BceAI	ACGGC	4 cut(s)	452, 712, 874, 1798
BciT130I	CCWGG	3 cut(s)	640, 996, 1777
BciVI	GTATCC	1 cut(s)	1201
BcnI	CCSGG	1 cut(s)	527
BcoDI	GTCTC	4 cut(s)	139, 260, 1391, 1445
BfaI	CTAG	6 cut(s)	111, 186, 230, 335, 1286, 1329
BfmI	CTRYAG	1 cut(s)	970
BfoI	RGCGCY	1 cut(s)	1788
BfuI	GTATCC	1 cut(s)	1201
BisI	GCNGC	9 cut(s)	292, 326, 351, 459, 816, 830, 1374, 1392, 1614
BlnI	CCTAGG	1 cut(s)	110
BlpI	GCTNAGC	1 cut(s)	1239
BlsI	GCNGC	9 cut(s)	293, 327, 352, 460, 817, 831, 1375, 1393, 1615
Bme1390I	CCNGG	4 cut(s)	527, 640, 996, 1777
Bme18I	GGWCC	4 cut(s)	1028, 1044, 1625, 1717
BmeRI	GACNNNNNGTC	1 cut(s)	1341
BmeT110I	CYCGRG	1 cut(s)	617
BmgBI	CACGTC	2 cut(s)	1343, 1597
BmgT120I	GGNCC	5 cut(s)	999, 1028, 1044, 1625, 1717
BmiI	GGNNCC	4 cut(s)	1000, 1107, 1617, 1627
BmrFI	CCNGG	4 cut(s)	527, 640, 996, 1777
BmrI	ACTGGG	1 cut(s)	748
BmsI	GCATC	3 cut(s)	874, 1600, 1818
BmuI	ACTGGG	1 cut(s)	748
BpiI	GAAGAC	2 cut(s)	244, 1080
BplI	GAGNNNNNCTC	2 cut(s)	1252, 1284
BpmI	CTGGAG	2 cut(s)	204, 1398
Bpu10I	CCTNAGC	1 cut(s)	1788
Bpu1102I	GCTNAGC	1 cut(s)	1239
BpuMI	CCSGG	1 cut(s)	527
Bsa29I	ATCGAT	1 cut(s)	843
BsaAI	YACGTR	1 cut(s)	1296
BsaHI	GRCGYC	2 cut(s)	1337, 1403
BsaI	GGTCTC	1 cut(s)	260
BsaJI	CCNNGG	6 cut(s)	110, 468, 879, 995, 1316, 1775
BsaWI	WCCGGW	2 cut(s)	1030, 1046
Bsc4I	CCNNNNNNNGG	5 cut(s)	995, 1001, 1101, 1726, 1781
Bse118I	RCCGGY	2 cut(s)	454, 1001
Bse1I	ACTGG	3 cut(s)	221, 317, 743
BseBI	CCWGG	3 cut(s)	640, 996, 1777
BseCI	ATCGAT	1 cut(s)	843
BseDI	CCNNGG	6 cut(s)	110, 468, 879, 995, 1316, 1775
BseGI	GGATG	6 cut(s)	45, 871, 889, 1522, 1606, 1615
BseLI	CCNNNNNNNGG	5 cut(s)	995, 1001, 1101, 1726, 1781
BseMII	CTCAG	3 cut(s)	1230, 1386, 1802
BseNI	ACTGG	3 cut(s)	221, 317, 743
BseRI	GAGGAG	3 cut(s)	1510, 1535, 1538
BseSI	GKGCMC	1 cut(s)	1776
BseXI	GCAGC	4 cut(s)	303, 337, 470, 802
BseYI	CCCAGC	2 cut(s)	552, 1646
Bsh1236I	CGCG	5 cut(s)	447, 541, 722, 746, 813
BshFI	GGCC	5 cut(s)	454, 695, 1001, 1391, 1480
BshNI	GGYRCC	1 cut(s)	1615
BshVI	ATCGAT	1 cut(s)	843
BsiHKAI	GWGCWC	3 cut(s)	513, 624, 810
BsiHKCI	CYCGRG	1 cut(s)	617
BsiSI	CCGG	5 cut(s)	455, 527, 1002, 1031, 1047
BsiWI	CGTACG	1 cut(s)	591
BslFI	GGGAC	5 cut(s)	359, 385, 755, 1183, 1277
BslI	CCNNNNNNNGG	5 cut(s)	995, 1001, 1101, 1726, 1781
BsmAI	GTCTC	4 cut(s)	139, 260, 1391, 1445
BsmBI	CGTCTC	2 cut(s)	1391, 1445
BsmFI	GGGAC	5 cut(s)	359, 385, 755, 1183, 1277
BsmI	GAATGC	2 cut(s)	115, 1240
BsnI	GGCC	5 cut(s)	454, 695, 1001, 1391, 1480
Bso31I	GGTCTC	1 cut(s)	260
BsoBI	CYCGRG	1 cut(s)	617
Bsp1286I	GDGCHC	4 cut(s)	513, 624, 810, 1776
Bsp143I	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
Bsp1720I	GCTNAGC	1 cut(s)	1239
Bsp19I	CCATGG	1 cut(s)	1316
BspANI	GGCC	5 cut(s)	454, 695, 1001, 1391, 1480
BspCNI	CTCAG	3 cut(s)	1231, 1387, 1801
BspDI	ATCGAT	1 cut(s)	843
BspFNI	CGCG	5 cut(s)	447, 541, 722, 746, 813
BspHI	TCATGA	1 cut(s)	513
BspLI	GGNNCC	4 cut(s)	1000, 1107, 1617, 1627
BspPI	GGATC	6 cut(s)	530, 704, 986, 1100, 1113, 1146
BspT107I	GGYRCC	1 cut(s)	1615
BspTNI	GGTCTC	1 cut(s)	260
BsrFI	RCCGGY	2 cut(s)	454, 1001
BsrI	ACTGG	3 cut(s)	221, 317, 743
BssAI	RCCGGY	2 cut(s)	454, 1001
BssECI	CCNNGG	6 cut(s)	110, 468, 879, 995, 1316, 1775
BssMI	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
BssNAI	GTATAC	1 cut(s)	1293
BssNI	GRCGYC	2 cut(s)	1337, 1403
BssT1I	CCWWGG	3 cut(s)	110, 879, 1316
Bst1107I	GTATAC	1 cut(s)	1293
Bst2UI	CCWGG	3 cut(s)	640, 996, 1777
Bst4CI	ACNGT	6 cut(s)	272, 386, 572, 587, 1258, 1468
Bst6I	CTCTTC	1 cut(s)	1487
BstACI	GRCGYC	2 cut(s)	1337, 1403
BstBAI	YACGTR	1 cut(s)	1296
BstC8I	GCNNGC	9 cut(s)	392, 456, 558, 750, 754, 758, 820, 979, 1356
BstDEI	CTNAG	3 cut(s)	1239, 1395, 1788
BstDSI	CCRYGG	2 cut(s)	468, 1316
BstENI	CCTNNNNNAGG	1 cut(s)	1724
BstF5I	GGATG	6 cut(s)	45, 871, 889, 1522, 1606, 1615
BstFNI	CGCG	5 cut(s)	447, 541, 722, 746, 813
BstH2I	RGCGCY	1 cut(s)	1788
BstHHI	GCGC	4 cut(s)	748, 815, 1123, 1787
BstKTI	GATC	8 cut(s)	199, 538, 712, 847, 994, 1108, 1141, 1803
BstMAI	GTCTC	4 cut(s)	139, 260, 1391, 1445
BstMBI	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
BstMWI	GCNNNNNNNGC	9 cut(s)	322, 464, 776, 819, 1364, 1373, 1382, 1391, 1672
BstNI	CCWGG	3 cut(s)	640, 996, 1777
BstNSI	RCATGY	2 cut(s)	245, 760
BstSCI	CCNGG	4 cut(s)	525, 638, 994, 1775
BstSFI	CTRYAG	1 cut(s)	970
BstSLI	GKGCMC	1 cut(s)	1776
BstSNI	TACGTA	1 cut(s)	1296
BstUI	CGCG	5 cut(s)	447, 541, 722, 746, 813
BstV1I	GCAGC	4 cut(s)	303, 337, 470, 802
BstV2I	GAAGAC	2 cut(s)	244, 1080
BstX2I	RGATCY	2 cut(s)	991, 1105
BstYI	RGATCY	2 cut(s)	991, 1105
BstZ17I	GTATAC	1 cut(s)	1293
Bsu15I	ATCGAT	1 cut(s)	843
BsuI	GTATCC	1 cut(s)	1201
BsuRI	GGCC	5 cut(s)	454, 695, 1001, 1391, 1480
BsuTUI	ATCGAT	1 cut(s)	843
BtgI	CCRYGG	2 cut(s)	468, 1316
BtrI	CACGTC	2 cut(s)	1343, 1597
BtsCI	GGATG	6 cut(s)	45, 871, 889, 1522, 1606, 1615
BtsI	GCAGTG	1 cut(s)	1095
BtsIMutI	CAGTG	4 cut(s)	324, 568, 981, 1095
Cac8I	GCNNGC	9 cut(s)	392, 456, 558, 750, 754, 758, 820, 979, 1356
CciI	TCATGA	1 cut(s)	513
CfoI	GCGC	4 cut(s)	748, 815, 1123, 1787
Cfr10I	RCCGGY	2 cut(s)	454, 1001
Cfr13I	GGNCC	5 cut(s)	999, 1028, 1044, 1625, 1717
ClaI	ATCGAT	1 cut(s)	843
CseI	GACGC	3 cut(s)	436, 752, 1345
Csp6I	GTAC	5 cut(s)	245, 268, 592, 775, 1780
CviQI	GTAC	5 cut(s)	245, 268, 592, 775, 1780
DdeI	CTNAG	3 cut(s)	1239, 1395, 1788
DpnI	GATC	8 cut(s)	198, 537, 711, 846, 993, 1107, 1140, 1802
DpnII	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
DraIII	CACNNNGTG	2 cut(s)	469, 1622
DriI	GACNNNNNGTC	1 cut(s)	1341
EaeI	YGGCCR	1 cut(s)	693
Eam1104I	CTCTTC	1 cut(s)	1487
Eam1105I	GACNNNNNGTC	1 cut(s)	1341
EarI	CTCTTC	1 cut(s)	1487
Ecl136II	GAGCTC	3 cut(s)	511, 622, 808
Eco105I	TACGTA	1 cut(s)	1296
Eco130I	CCWWGG	3 cut(s)	110, 879, 1316
Eco24I	GRGCYC	3 cut(s)	513, 624, 810
Eco31I	GGTCTC	1 cut(s)	260
Eco47I	GGWCC	4 cut(s)	1028, 1044, 1625, 1717
Eco53kI	GAGCTC	3 cut(s)	511, 622, 808
Eco57I	CTGAAG	1 cut(s)	1761
Eco88I	CYCGRG	1 cut(s)	617
EcoICRI	GAGCTC	3 cut(s)	511, 622, 808
EcoNI	CCTNNNNNAGG	1 cut(s)	1724
EcoRII	CCWGG	3 cut(s)	638, 994, 1775
EcoT14I	CCWWGG	3 cut(s)	110, 879, 1316
EcoT38I	GRGCYC	3 cut(s)	513, 624, 810
ErhI	CCWWGG	3 cut(s)	110, 879, 1316
Esp3I	CGTCTC	2 cut(s)	1391, 1445
FaqI	GGGAC	5 cut(s)	359, 385, 755, 1183, 1277
FauI	CCCGC	2 cut(s)	399, 1417
FblI	GTMKAC	2 cut(s)	414, 1292
Fnu4HI	GCNGC	9 cut(s)	292, 326, 351, 459, 816, 830, 1374, 1392, 1614
FokI	GGATG	6 cut(s)	32, 878, 896, 1529, 1613, 1622
FriOI	GRGCYC	3 cut(s)	513, 624, 810
Fsp4HI	GCNGC	9 cut(s)	292, 326, 351, 459, 816, 830, 1374, 1392, 1614
FspBI	CTAG	6 cut(s)	111, 186, 230, 335, 1286, 1329
FspI	TGCGCA	1 cut(s)	1122
GlaI	GCGC	4 cut(s)	747, 814, 1122, 1786
GluI	GCNGC	9 cut(s)	292, 326, 351, 459, 816, 830, 1374, 1392, 1614
GsaI	CCCAGC	2 cut(s)	556, 1650
GsuI	CTGGAG	2 cut(s)	204, 1398
HaeII	RGCGCY	1 cut(s)	1788
HaeIII	GGCC	5 cut(s)	454, 695, 1001, 1391, 1480
HapII	CCGG	5 cut(s)	455, 527, 1002, 1031, 1047
HgaI	GACGC	3 cut(s)	436, 752, 1345
HhaI	GCGC	4 cut(s)	748, 815, 1123, 1787
Hin1I	GRCGYC	2 cut(s)	1337, 1403
Hin6I	GCGC	4 cut(s)	746, 813, 1121, 1785
HinP1I	GCGC	4 cut(s)	746, 813, 1121, 1785
HincII	GTYRAC	1 cut(s)	1713
HindII	GTYRAC	1 cut(s)	1713
HindIII	AAGCTT	1 cut(s)	1352
HinfI	GANTC	5 cut(s)	687, 788, 1132, 1312, 1537
HpaI	GTTAAC	1 cut(s)	1713
HpaII	CCGG	5 cut(s)	455, 527, 1002, 1031, 1047
HphI	GGTGA	6 cut(s)	19, 77, 541, 1270, 1546, 1774
Hpy166II	GTNNAC	6 cut(s)	226, 415, 568, 1293, 1625, 1713
Hpy188I	TCNGA	4 cut(s)	260, 714, 1110, 1347
Hpy188III	TCNNGA	7 cut(s)	200, 376, 514, 848, 1136, 1271, 1329
Hpy8I	GTNNAC	6 cut(s)	226, 415, 568, 1293, 1625, 1713
Hpy99I	CGWCG	5 cut(s)	536, 806, 1339, 1347, 1405
HpyAV	CCTTC	9 cut(s)	189, 225, 310, 664, 727, 1381, 1408, 1498, 1672
HpyCH4III	ACNGT	6 cut(s)	272, 386, 572, 587, 1258, 1468
HpyCH4IV	ACGT	5 cut(s)	398, 1295, 1342, 1403, 1596
HpyCH4V	TGCA	5 cut(s)	141, 311, 760, 1007, 1100
HpyF10VI	GCNNNNNNNGC	9 cut(s)	322, 464, 776, 819, 1364, 1373, 1382, 1391, 1672
HpyF3I	CTNAG	3 cut(s)	1239, 1395, 1788
HpySE526I	ACGT	5 cut(s)	398, 1295, 1342, 1403, 1596
Hsp92I	GRCGYC	2 cut(s)	1337, 1403
HspAI	GCGC	4 cut(s)	746, 813, 1121, 1785
KroI	GCCGGC	1 cut(s)	454
KroNI	GCCGGC	1 cut(s)	456
KspAI	GTTAAC	1 cut(s)	1713
Kzo9I	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
LmnI	GCTCC	7 cut(s)	22, 194, 347, 368, 627, 813, 1497
Lsp1109I	GCAGC	4 cut(s)	303, 337, 470, 802
LweI	GCATC	3 cut(s)	874, 1600, 1818
MaeI	CTAG	6 cut(s)	111, 186, 230, 335, 1286, 1329
MaeII	ACGT	5 cut(s)	398, 1295, 1342, 1403, 1596
MaeIII	GTNAC	6 cut(s)	25, 161, 581, 662, 1252, 1762
MalI	GATC	8 cut(s)	198, 537, 711, 846, 993, 1107, 1140, 1802
MboI	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
MboII	GAAGA	8 cut(s)	249, 640, 1085, 1313, 1468, 1501, 1504, 1754
MflI	RGATCY	2 cut(s)	991, 1105
MhlI	GDGCHC	4 cut(s)	513, 624, 810, 1776
MlsI	TGGCCA	1 cut(s)	695
MluCI	AATT	6 cut(s)	121, 422, 1066, 1084, 1444, 1815
MluNI	TGGCCA	1 cut(s)	695
MlyI	GAGTC	1 cut(s)	782
MmeI	TCCRAC	6 cut(s)	216, 737, 957, 1185, 1325, 1704
Mox20I	TGGCCA	1 cut(s)	695
MroNI	GCCGGC	1 cut(s)	454
MscI	TGGCCA	1 cut(s)	695
MseI	TTAA	4 cut(s)	1188, 1700, 1712, 1797
MslI	CAYNNNNRTG	1 cut(s)	204
Msp20I	TGGCCA	1 cut(s)	695
MspA1I	CMGCKG	3 cut(s)	752, 949, 1394
MspI	CCGG	5 cut(s)	455, 527, 1002, 1031, 1047
MspR9I	CCNGG	4 cut(s)	527, 640, 996, 1777
Mva1269I	GAATGC	2 cut(s)	115, 1240
MvaI	CCWGG	3 cut(s)	640, 996, 1777
MvnI	CGCG	5 cut(s)	447, 541, 722, 746, 813
MwoI	GCNNNNNNNGC	9 cut(s)	322, 464, 776, 819, 1364, 1373, 1382, 1391, 1672
NaeI	GCCGGC	1 cut(s)	456
NciI	CCSGG	1 cut(s)	527
NcoI	CCATGG	1 cut(s)	1316
NdeII	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
NgoMIV	GCCGGC	1 cut(s)	454
NlaIV	GGNNCC	4 cut(s)	1000, 1107, 1617, 1627
NmuCI	GTSAC	4 cut(s)	25, 161, 1252, 1762
NsbI	TGCGCA	1 cut(s)	1122
NspI	RCATGY	2 cut(s)	245, 760
PaeI	GCATGC	1 cut(s)	760
PaeR7I	CTCGAG	1 cut(s)	617
PagI	TCATGA	1 cut(s)	513
PciI	ACATGT	1 cut(s)	241
PcsI	WCGNNNNNNNCGW	1 cut(s)	591
PctI	GAATGC	2 cut(s)	115, 1240
PdiI	GCCGGC	1 cut(s)	456
PfeI	GAWTC	4 cut(s)	687, 1132, 1312, 1537
Pfl23II	CGTACG	1 cut(s)	591
PflFI	GACNNNGTC	2 cut(s)	599, 1402
PfoI	TCCNGGA	1 cut(s)	525
PkrI	GCNGC	9 cut(s)	293, 327, 352, 460, 817, 831, 1375, 1393, 1615
PleI	GAGTC	1 cut(s)	782
PpsI	GAGTC	1 cut(s)	782
Ppu21I	YACGTR	1 cut(s)	1296
PscI	ACATGT	1 cut(s)	241
Psp124BI	GAGCTC	3 cut(s)	513, 624, 810
Psp6I	CCWGG	3 cut(s)	638, 994, 1775
PspFI	CCCAGC	2 cut(s)	552, 1646
PspGI	CCWGG	3 cut(s)	638, 994, 1775
PspLI	CGTACG	1 cut(s)	591
PspN4I	GGNNCC	4 cut(s)	1000, 1107, 1617, 1627
PspPI	GGNCC	5 cut(s)	999, 1028, 1044, 1625, 1717
PspXI	VCTCGAGB	1 cut(s)	617
PsuI	RGATCY	2 cut(s)	991, 1105
PsyI	GACNNNGTC	2 cut(s)	599, 1402
PvuII	CAGCTG	1 cut(s)	752
RsaI	GTAC	5 cut(s)	246, 269, 593, 776, 1781
RsaNI	GTAC	5 cut(s)	245, 268, 592, 775, 1780
RseI	CAYNNNNRTG	1 cut(s)	204
SacI	GAGCTC	3 cut(s)	513, 624, 810
SaqAI	TTAA	4 cut(s)	1188, 1700, 1712, 1797
SatI	GCNGC	9 cut(s)	292, 326, 351, 459, 816, 830, 1374, 1392, 1614
Sau3AI	GATC	8 cut(s)	196, 535, 709, 844, 991, 1105, 1138, 1800
Sau96I	GGNCC	5 cut(s)	999, 1028, 1044, 1625, 1717
SchI	GAGTC	1 cut(s)	782
ScrFI	CCNGG	4 cut(s)	527, 640, 996, 1777
SduI	GDGCHC	4 cut(s)	513, 624, 810, 1776
SfaNI	GCATC	3 cut(s)	874, 1600, 1818
SfcI	CTRYAG	1 cut(s)	970
Sfr274I	CTCGAG	1 cut(s)	617
SinI	GGWCC	4 cut(s)	1028, 1044, 1625, 1717
SlaI	CTCGAG	1 cut(s)	617
SmiMI	CAYNNNNRTG	1 cut(s)	204
SmlI	CTYRAG	1 cut(s)	617
SmoI	CTYRAG	1 cut(s)	617
SnaBI	TACGTA	1 cut(s)	1296
SphI	GCATGC	1 cut(s)	760
Sse9I	AATT	6 cut(s)	121, 422, 1066, 1084, 1444, 1815
SspI	AATATT	1 cut(s)	1153
SspMI	CTAG	6 cut(s)	111, 186, 230, 335, 1286, 1329
SstI	GAGCTC	3 cut(s)	513, 624, 810
StyD4I	CCNGG	4 cut(s)	525, 638, 994, 1775
StyI	CCWWGG	3 cut(s)	110, 879, 1316
TaaI	ACNGT	6 cut(s)	272, 386, 572, 587, 1258, 1468
TaiI	ACGT	5 cut(s)	401, 1298, 1345, 1406, 1599
TaqI	TCGA	7 cut(s)	486, 507, 618, 843, 894, 1011, 1141
TaqII	GACCGA	1 cut(s)	1348
TasI	AATT	6 cut(s)	121, 422, 1066, 1084, 1444, 1815
TatI	WGTACW	1 cut(s)	244
TauI	GCSGC	5 cut(s)	328, 832, 1376, 1394, 1616
TfiI	GAWTC	4 cut(s)	687, 1132, 1312, 1537
Tru1I	TTAA	4 cut(s)	1188, 1700, 1712, 1797
Tru9I	TTAA	4 cut(s)	1188, 1700, 1712, 1797
TscAI	CASTG	4 cut(s)	324, 575, 988, 1102
TseFI	GTSAC	4 cut(s)	25, 161, 1252, 1762
TseI	GCWGC	4 cut(s)	291, 350, 458, 815
Tsp45I	GTSAC	4 cut(s)	25, 161, 1252, 1762
TspDTI	ATGAA	6 cut(s)	222, 530, 882, 1025, 1086, 1314
TspGWI	ACGGA	2 cut(s)	878, 1730
TspRI	CASTG	4 cut(s)	324, 575, 988, 1102
Tth111I	GACNNNGTC	2 cut(s)	599, 1402
VpaK11BI	GGWCC	4 cut(s)	1028, 1044, 1625, 1717
XagI	CCTNNNNNAGG	1 cut(s)	1724
XapI	RAATTY	3 cut(s)	422, 1084, 1444
XbaI	TCTAGA	1 cut(s)	1328
XceI	RCATGY	2 cut(s)	245, 760
XhoI	CTCGAG	1 cut(s)	617
XmaJI	CCTAGG	1 cut(s)	110
XmiI	GTMKAC	2 cut(s)	414, 1292
XspI	CTAG	6 cut(s)	111, 186, 230, 335, 1286, 1329
ZraI	GACGTC	1 cut(s)	1404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.