Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Forward (+)

38683770 .. 38685551

Gene Structure 1782 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1299400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1782 bp

ATGACGTCTCTCTCACCTCCGGTAGTCACCACCCCCACCGTTCCCAACCCCGCCACAAAACCTCTCTCCCCCTTCTCTCAAAACAACTCCCAAGTTTCCCTACTCACAAAGCCCAAGCGTTCCTTTGCACGTAAGGTCTCATGCAAAGCCACAAACAATGACCAAAATGATCAAGCACAGTCCAAACTAGACAGGAGAAATGTGCTTCTTGGTCTTGGAGGTCTATACGGCGTGGCGGGTATGGGCACAGACCCGTTCGCTTTTGCCAAGCCTATAGCCCCACCAGACGTATCTAAATGTGGTCCTGCAGACTTGCCACAGGGTGCAGTGCCCACCAACTGCTGCCCGCCGCCTTCCACAAAAATCATTGACTTTAAGCTGCCTGCCCCCGCCAAACTCCGCATCAGGCCACCGGCTCACGCCGTTGACCAAGCCTACAGGGACAAATACTACAAAGCGATGGAGCTCATGAAGGCCCTACCCGACGACGACCCACGTAGCTTCAAGCAACAGGCAGCCGTGCATTGCGCTTATTGCGACGGCGCCTATGACCAAGTCGGGTTCCCAGAACTCGAGCTCCAAATCCACAACTCATGGCTCTTCTTCCCGTTCCACCGTTACTACTTGTACTTTTTCGAGAAGATCCTAGGCAAACTCATTAACGACCCGACATTCGCTTTGCCGTTCTGGAACTGGGACTCGCCAGCCGGCATGCCACTGCCCGCGATTTACGCTGATCCAAAGTCCCCTCTCTACGACAAGCTCCGATCTGCCAATCATCAGCCCCCGACTCTGGTCGATCTCGATTACAACGGGACCGAGGACAATGTGTCAAAGGAAACCACAATCAACGCCAATCTCAAAATCATGTACAGGCAAATGGTGTCCAATTCCAAGAATGCTAAGTTGTTCTTTGGGAACCCGTACAGGGCAGGGGACGAGCCTGACCCTGGTGGCGGCTCCATCGAGGGGACACCACACGCGCCGGTTCATTTATGGACCGGTGACAACACCCAGCCCAACTTTGAGGACATGGGGAATTTTTACTCCGCTGGTCGGGACCCCATATTTTTTGCACACCATTCGAATGTCGATCGAATGTGGAGTATTTGGAAAACTCTTGGAGGTAAGAGAACTGATCTTACTGACTCGGACTGGTTGGACTCCGGATTCTTGTTTTACAACGAGAACGCAGAGTTAGTCCGAGTCAAGGTCAGGGACTGCTTGGAGACCAAAAATCTTGGGTATGTATACCAAGATGTGGACATTCCTTGGCTCAGCTCCAAGCCAACACCGCGAAGGGCGAAAGTTGCATTGAGCAAAGTAGCGAAGAAGCTGGGAGTTGCACACGCAGCTGTTGCGTCGTCCAGCAAGGTGGTGGCAGGCACTGAGTTCCCGATAAGTCTGGGGTCGAAGATAAGCACGGTGGTGAAGAGACCGAAGCAGAAGAAGAGGAGCAAGAAGGCCAAGGAGGATGAGGAGGAGATATTGGTGATTGAGGGAATCGAGTTTGACAGGGACGTGGCTGTGAAGTTTGATGTGTATGTGAATGACGTCGATGACTTGCCGAGTGGGCCTGACAAGACGGAGTTTGCCGGAAGCTTTGTAAGTGTGCCGCACAGCCACAAGCACAAGAAGAAGATGAACACTATTTTGAGGTTAGGGTTGACAGATTTGTTGGAGGAAATTGAGGCGGAGGACGATGACAGCGTGGTGGTGACTTTGGTGCCCAAGTTCGGCGCTGTCAAGATTGGTGGTATCAAGATTGAATTTGCTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

594

Amino Acids

65.72

Weight (kDa)

7.97

Isoelectric Point (pI)

46.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	166 - 373	8.4e-38	Common central domain of tyrosinase
PPO1_DWL	PF12142	380 - 431	4.9e-26	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	465 - 591	1.5e-51	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	8, 1557
AccB1I	GGYRCC	2 cut(s)	542, 1726
AccB7I	CCANNNNNTGG	1 cut(s)	1261
AccI	GTMKAC	1 cut(s)	1251
AccII	CGCG	3 cut(s)	725, 983, 1297
AccIII	TCCGGA	1 cut(s)	1166
AclWI	GGATC	2 cut(s)	637, 731
AcsI	RAATTY	2 cut(s)	1039, 1769
AcyI	GRCGYC	3 cut(s)	5, 543, 1554
AdeI	CACNNNGTG	1 cut(s)	323
AfaI	GTAC	3 cut(s)	629, 872, 926
AfiI	CCNNNNNNNGG	9 cut(s)	793, 928, 950, 956, 969, 1056, 1210, 1261, 1736
AgeI	ACCGGT	1 cut(s)	1001
AgsI	TTSAA	2 cut(s)	505, 1769
AhdI	GACNNNNNGTC	1 cut(s)	829
AjiI	CACGTC	1 cut(s)	1522
AjnI	CCWGG	1 cut(s)	949
AleI	CACNNNNGTG	1 cut(s)	1427
AloI	GAACNNNNNNTCC	2 cut(s)	561, 593
AluBI	AGCT	9 cut(s)	379, 466, 501, 577, 763, 1281, 1336, 1355, 1602
AluI	AGCT	9 cut(s)	379, 466, 501, 577, 763, 1281, 1336, 1355, 1602
Alw21I	GWGCWC	2 cut(s)	468, 579
Alw26I	GTCTC	4 cut(s)	12, 142, 1223, 1429
AlwI	GGATC	2 cut(s)	637, 731
AlwNI	CAGNNNCTG	2 cut(s)	1221, 1388
Ama87I	CYCGRG	1 cut(s)	572
Aor13HI	TCCGGA	1 cut(s)	1166
AoxI	GGCC	4 cut(s)	407, 474, 1464, 1574
ApeKI	GCWGC	4 cut(s)	342, 379, 515, 1352
ApoI	RAATTY	2 cut(s)	1039, 1769
ArsI	GACNNNNNNTTYG	4 cut(s)	656, 661, 688, 693
AsiGI	ACCGGT	1 cut(s)	1001
AspA2I	CCTAGG	1 cut(s)	646
AspLEI	GCGC	4 cut(s)	530, 545, 985, 1742
AspS9I	GGNCC	6 cut(s)	302, 475, 816, 999, 1060, 1574
AsuHPI	GGTGA	6 cut(s)	6, 19, 1016, 1441, 1504, 1729
AsuII	TTCGAA	1 cut(s)	1085
AvaI	CYCGRG	1 cut(s)	572
AvaII	GGWCC	4 cut(s)	302, 816, 999, 1060
AvrII	CCTAGG	1 cut(s)	646
BaeGI	GKGCMC	3 cut(s)	248, 333, 1731
BanI	GGYRCC	2 cut(s)	542, 1726
BanII	GRGCYC	2 cut(s)	468, 579
Bbv12I	GWGCWC	2 cut(s)	468, 579
BbvI	GCAGC	4 cut(s)	329, 366, 527, 1364
BccI	CCATC	2 cut(s)	454, 971
BceAI	ACGGC	5 cut(s)	244, 407, 503, 556, 667
BcgI	CGANNNNNNTGC	2 cut(s)	1065, 1099
BciT130I	CCWGG	1 cut(s)	951
BclI	TGATCA	1 cut(s)	169
BcoDI	GTCTC	4 cut(s)	12, 142, 1223, 1429
BfaI	CTAG	2 cut(s)	188, 647
BfmI	CTRYAG	3 cut(s)	273, 306, 436
BfoI	RGCGCY	2 cut(s)	546, 1743
BglI	GCCNNNNNGGC	1 cut(s)	1573
BisI	GCNGC	7 cut(s)	343, 350, 380, 516, 958, 1353, 1616
BlnI	CCTAGG	1 cut(s)	646
BlpI	GCTNAGC	1 cut(s)	1277
BlsI	GCNGC	7 cut(s)	344, 351, 381, 517, 959, 1354, 1617
Bme1390I	CCNGG	1 cut(s)	951
Bme18I	GGWCC	4 cut(s)	302, 816, 999, 1060
BmeRI	GACNNNNNGTC	1 cut(s)	829
BmeT110I	CYCGRG	1 cut(s)	572
BmgBI	CACGTC	1 cut(s)	1522
BmgT120I	GGNCC	6 cut(s)	302, 475, 816, 999, 1060, 1574
BmiI	GGNNCC	8 cut(s)	544, 563, 817, 920, 961, 1061, 1062, 1728
BmrFI	CCNGG	1 cut(s)	951
BmrI	ACTGGG	1 cut(s)	703
BmsI	GCATC	1 cut(s)	411
BmuI	ACTGGG	1 cut(s)	703
BoxI	GACNNNNGTC	1 cut(s)	794
Bpu1102I	GCTNAGC	1 cut(s)	1277
Bpu14I	TTCGAA	1 cut(s)	1085
BsaAI	YACGTR	2 cut(s)	131, 497
BsaHI	GRCGYC	3 cut(s)	5, 543, 1554
BsaI	GGTCTC	3 cut(s)	142, 1223, 1429
BsaJI	CCNNGG	5 cut(s)	646, 819, 949, 1271, 1467
BsaWI	WCCGGW	3 cut(s)	19, 1001, 1166
BsaXI	ACNNNNNCTCC	2 cut(s)	561, 591
Bsc4I	CCNNNNNNNGG	9 cut(s)	793, 928, 950, 956, 969, 1056, 1210, 1261, 1736
Bse118I	RCCGGY	4 cut(s)	412, 707, 985, 1001
Bse1I	ACTGG	2 cut(s)	698, 1160
Bse3DI	GCAATG	1 cut(s)	523
BseAI	TCCGGA	1 cut(s)	1166
BseBI	CCWGG	1 cut(s)	951
BseDI	CCNNGG	5 cut(s)	646, 819, 949, 1271, 1467
BseGI	GGATG	1 cut(s)	1480
BseLI	CCNNNNNNNGG	9 cut(s)	793, 928, 950, 956, 969, 1056, 1210, 1261, 1736
BseMI	GCAATG	1 cut(s)	523
BseMII	CTCAG	2 cut(s)	1291, 1380
BseNI	ACTGG	2 cut(s)	698, 1160
BseRI	GAGGAG	3 cut(s)	1468, 1493, 1496
BseSI	GKGCMC	3 cut(s)	248, 333, 1731
BseXI	GCAGC	4 cut(s)	329, 366, 527, 1364
BseYI	CCCAGC	2 cut(s)	1014, 1336
BsgI	GTGCAG	1 cut(s)	345
Bsh1236I	CGCG	3 cut(s)	725, 983, 1297
Bsh1285I	CGRYCG	1 cut(s)	1096
BshFI	GGCC	4 cut(s)	409, 476, 1466, 1576
BshNI	GGYRCC	2 cut(s)	542, 1726
BshTI	ACCGGT	1 cut(s)	1001
BsiEI	CGRYCG	1 cut(s)	1096
BsiHKAI	GWGCWC	2 cut(s)	468, 579
BsiHKCI	CYCGRG	1 cut(s)	572
BsiSI	CCGG	7 cut(s)	20, 413, 708, 986, 1002, 1167, 1596
BslFI	GGGAC	9 cut(s)	455, 710, 730, 829, 950, 985, 1073, 1232, 1532
BslI	CCNNNNNNNGG	9 cut(s)	793, 928, 950, 956, 969, 1056, 1210, 1261, 1736
BsmAI	GTCTC	4 cut(s)	12, 142, 1223, 1429
BsmBI	CGTCTC	1 cut(s)	12
BsmFI	GGGAC	9 cut(s)	455, 710, 730, 829, 950, 985, 1073, 1232, 1532
BsmI	GAATGC	1 cut(s)	904
BsnI	GGCC	4 cut(s)	409, 476, 1466, 1576
Bso31I	GGTCTC	3 cut(s)	142, 1223, 1429
BsoBI	CYCGRG	1 cut(s)	572
Bsp119I	TTCGAA	1 cut(s)	1085
Bsp1286I	GDGCHC	5 cut(s)	248, 333, 468, 579, 1731
Bsp13I	TCCGGA	1 cut(s)	1166
Bsp1407I	TGTACA	1 cut(s)	870
Bsp143I	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
Bsp1720I	GCTNAGC	1 cut(s)	1277
BspANI	GGCC	4 cut(s)	409, 476, 1466, 1576
BspCNI	CTCAG	2 cut(s)	1290, 1381
BspEI	TCCGGA	1 cut(s)	1166
BspFNI	CGCG	3 cut(s)	725, 983, 1297
BspHI	TCATGA	1 cut(s)	468
BspLI	GGNNCC	8 cut(s)	544, 563, 817, 920, 961, 1061, 1062, 1728
BspMAI	CTGCAG	1 cut(s)	310
BspPI	GGATC	2 cut(s)	637, 731
BspQI	GCTCTTC	1 cut(s)	605
BspT104I	TTCGAA	1 cut(s)	1085
BspT107I	GGYRCC	2 cut(s)	542, 1726
BspTNI	GGTCTC	3 cut(s)	142, 1223, 1429
BsrDI	GCAATG	1 cut(s)	523
BsrFI	RCCGGY	4 cut(s)	412, 707, 985, 1001
BsrGI	TGTACA	1 cut(s)	870
BsrI	ACTGG	2 cut(s)	698, 1160
BssAI	RCCGGY	4 cut(s)	412, 707, 985, 1001
BssECI	CCNNGG	5 cut(s)	646, 819, 949, 1271, 1467
BssMI	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
BssNAI	GTATAC	1 cut(s)	1252
BssNI	GRCGYC	3 cut(s)	5, 543, 1554
BssT1I	CCWWGG	3 cut(s)	646, 1271, 1467
Bst1107I	GTATAC	1 cut(s)	1252
Bst2UI	CCWGG	1 cut(s)	951
Bst4CI	ACNGT	4 cut(s)	40, 180, 617, 1426
Bst6I	CTCTTC	3 cut(s)	605, 1427, 1445
BstACI	GRCGYC	3 cut(s)	5, 543, 1554
BstAPI	GCANNNNNTGC	1 cut(s)	1358
BstAUI	TGTACA	1 cut(s)	870
BstBAI	YACGTR	2 cut(s)	131, 497
BstBI	TTCGAA	1 cut(s)	1085
BstC8I	GCNNGC	7 cut(s)	347, 384, 705, 709, 713, 723, 1384
BstDEI	CTNAG	4 cut(s)	903, 1277, 1389, 1779
BstF5I	GGATG	1 cut(s)	1480
BstFNI	CGCG	3 cut(s)	725, 983, 1297
BstH2I	RGCGCY	2 cut(s)	546, 1743
BstHHI	GCGC	4 cut(s)	530, 545, 985, 1742
BstKTI	GATC	7 cut(s)	172, 645, 739, 770, 802, 1096, 1141
BstMAI	GTCTC	4 cut(s)	12, 142, 1223, 1429
BstMBI	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
BstMCI	CGRYCG	1 cut(s)	1096
BstMWI	GCNNNNNNNGC	7 cut(s)	534, 731, 1294, 1310, 1352, 1358, 1573
BstNI	CCWGG	1 cut(s)	951
BstNSI	RCATGY	1 cut(s)	715
BstPAI	GACNNNNGTC	1 cut(s)	794
BstSCI	CCNGG	1 cut(s)	949
BstSFI	CTRYAG	3 cut(s)	273, 306, 436
BstSLI	GKGCMC	3 cut(s)	248, 333, 1731
BstUI	CGCG	3 cut(s)	725, 983, 1297
BstV1I	GCAGC	4 cut(s)	329, 366, 527, 1364
BstX2I	RGATCY	1 cut(s)	642
BstXI	CCANNNNNNTGG	1 cut(s)	1375
BstYI	RGATCY	1 cut(s)	642
BstZ17I	GTATAC	1 cut(s)	1252
BsuRI	GGCC	4 cut(s)	409, 476, 1466, 1576
BtgZI	GCGATG	1 cut(s)	473
BtrI	CACGTC	1 cut(s)	1522
BtsCI	GGATG	1 cut(s)	1480
BtsI	GCAGTG	2 cut(s)	333, 716
BtsIMutI	CAGTG	3 cut(s)	333, 716, 1386
Cac8I	GCNNGC	7 cut(s)	347, 384, 705, 709, 713, 723, 1384
CaiI	CAGNNNCTG	2 cut(s)	1221, 1388
CciI	TCATGA	1 cut(s)	468
CfoI	GCGC	4 cut(s)	530, 545, 985, 1742
Cfr10I	RCCGGY	4 cut(s)	412, 707, 985, 1001
Cfr13I	GGNCC	6 cut(s)	302, 475, 816, 999, 1060, 1574
CseI	GACGC	1 cut(s)	1350
Csp6I	GTAC	3 cut(s)	628, 871, 925
CspAI	ACCGGT	1 cut(s)	1001
CspCI	CAANNNNNGTGG	2 cut(s)	1735, 1770
CviAII	CATG	6 cut(s)	141, 469, 594, 712, 868, 1033
CviQI	GTAC	3 cut(s)	628, 871, 925
DdeI	CTNAG	4 cut(s)	903, 1277, 1389, 1779
DinI	GGCGCC	1 cut(s)	544
DpnI	GATC	7 cut(s)	171, 644, 738, 769, 801, 1095, 1140
DpnII	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
DraIII	CACNNNGTG	1 cut(s)	323
DriI	GACNNNNNGTC	1 cut(s)	829
Eam1104I	CTCTTC	3 cut(s)	605, 1427, 1445
Eam1105I	GACNNNNNGTC	1 cut(s)	829
EarI	CTCTTC	3 cut(s)	605, 1427, 1445
EciI	GGCGGA	1 cut(s)	1709
Ecl136II	GAGCTC	2 cut(s)	466, 577
Eco130I	CCWWGG	3 cut(s)	646, 1271, 1467
Eco24I	GRGCYC	2 cut(s)	468, 579
Eco31I	GGTCTC	3 cut(s)	142, 1223, 1429
Eco47I	GGWCC	4 cut(s)	302, 816, 999, 1060
Eco53kI	GAGCTC	2 cut(s)	466, 577
Eco88I	CYCGRG	1 cut(s)	572
EcoICRI	GAGCTC	2 cut(s)	466, 577
EcoO109I	RGGNCCY	2 cut(s)	475, 1060
EcoRII	CCWGG	1 cut(s)	949
EcoT14I	CCWWGG	3 cut(s)	646, 1271, 1467
EcoT38I	GRGCYC	2 cut(s)	468, 579
EgeI	GGCGCC	1 cut(s)	544
EheI	GGCGCC	1 cut(s)	544
ErhI	CCWWGG	3 cut(s)	646, 1271, 1467
Esp3I	CGTCTC	1 cut(s)	12
FaeI	CATG	6 cut(s)	144, 472, 597, 715, 871, 1036
FalI	AAGNNNNNCTT	4 cut(s)	107, 139, 896, 928
FaqI	GGGAC	9 cut(s)	455, 710, 730, 829, 950, 985, 1073, 1232, 1532
FatI	CATG	6 cut(s)	140, 468, 593, 711, 867, 1032
FauI	CCCGC	5 cut(s)	58, 229, 354, 397, 730
FbaI	TGATCA	1 cut(s)	169
FblI	GTMKAC	1 cut(s)	1251
Fnu4HI	GCNGC	7 cut(s)	343, 350, 380, 516, 958, 1353, 1616
FokI	GGATG	1 cut(s)	1487
FriOI	GRGCYC	2 cut(s)	468, 579
Fsp4HI	GCNGC	7 cut(s)	343, 350, 380, 516, 958, 1353, 1616
FspBI	CTAG	2 cut(s)	188, 647
GlaI	GCGC	4 cut(s)	529, 544, 984, 1741
GluI	GCNGC	7 cut(s)	343, 350, 380, 516, 958, 1353, 1616
GsaI	CCCAGC	2 cut(s)	1018, 1340
HaeII	RGCGCY	2 cut(s)	546, 1743
HaeIII	GGCC	4 cut(s)	409, 476, 1466, 1576
HapII	CCGG	7 cut(s)	20, 413, 708, 986, 1002, 1167, 1596
HgaI	GACGC	1 cut(s)	1350
HhaI	GCGC	4 cut(s)	530, 545, 985, 1742
Hin1I	GRCGYC	3 cut(s)	5, 543, 1554
Hin1II	CATG	6 cut(s)	144, 472, 597, 715, 871, 1036
Hin6I	GCGC	4 cut(s)	528, 543, 983, 1740
HinP1I	GCGC	4 cut(s)	528, 543, 983, 1740
HincII	GTYRAC	2 cut(s)	427, 1668
HindII	GTYRAC	2 cut(s)	427, 1668
HindIII	AAGCTT	1 cut(s)	1600
HinfI	GANTC	7 cut(s)	698, 790, 1148, 1163, 1170, 1206, 1503
HpaII	CCGG	7 cut(s)	20, 413, 708, 986, 1002, 1167, 1596
HphI	GGTGA	6 cut(s)	6, 19, 1016, 1441, 1504, 1729
Hpy166II	GTNNAC	4 cut(s)	427, 1252, 1264, 1668
Hpy188I	TCNGA	3 cut(s)	767, 1153, 1205
Hpy188III	TCNNGA	9 cut(s)	469, 637, 688, 803, 1058, 1167, 1396, 1747, 1762
Hpy8I	GTNNAC	4 cut(s)	427, 1252, 1264, 1668
Hpy99I	CGWCG	5 cut(s)	488, 491, 542, 1366, 1559
HpyAV	CCTTC	5 cut(s)	82, 363, 466, 1293, 1456
HpyCH4III	ACNGT	4 cut(s)	40, 180, 617, 1426
HpyCH4IV	ACGT	6 cut(s)	5, 130, 288, 496, 1521, 1554
HpyCH4V	TGCA	8 cut(s)	128, 144, 308, 326, 523, 1076, 1313, 1346
HpyF10VI	GCNNNNNNNGC	7 cut(s)	534, 731, 1294, 1310, 1352, 1358, 1573
HpyF3I	CTNAG	4 cut(s)	903, 1277, 1389, 1779
HpySE526I	ACGT	6 cut(s)	5, 130, 288, 496, 1521, 1554
Hsp92I	GRCGYC	3 cut(s)	5, 543, 1554
Hsp92II	CATG	6 cut(s)	144, 472, 597, 715, 871, 1036
HspAI	GCGC	4 cut(s)	528, 543, 983, 1740
KasI	GGCGCC	1 cut(s)	542
KflI	GGGWCCC	1 cut(s)	1060
Kpn2I	TCCGGA	1 cut(s)	1166
KroI	GCCGGC	1 cut(s)	707
KroNI	GCCGGC	1 cut(s)	709
Ksp22I	TGATCA	1 cut(s)	169
Kzo9I	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
LguI	GCTCTTC	1 cut(s)	605
LmnI	GCTCC	6 cut(s)	463, 582, 768, 965, 1286, 1455
Lsp1109I	GCAGC	4 cut(s)	329, 366, 527, 1364
LweI	GCATC	1 cut(s)	411
MaeI	CTAG	2 cut(s)	188, 647
MaeII	ACGT	6 cut(s)	5, 130, 288, 496, 1521, 1554
MaeIII	GTNAC	4 cut(s)	25, 617, 1004, 1717
MalI	GATC	7 cut(s)	171, 644, 738, 769, 801, 1095, 1140
MboI	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
MflI	RGATCY	1 cut(s)	642
MhlI	GDGCHC	5 cut(s)	248, 333, 468, 579, 1731
MluCI	AATT	4 cut(s)	889, 1039, 1686, 1769
Mly113I	GGCGCC	1 cut(s)	543
MlyI	GAGTC	5 cut(s)	692, 784, 1142, 1157, 1215
MmeI	TCCRAC	2 cut(s)	1140, 1659
MroI	TCCGGA	1 cut(s)	1166
MroNI	GCCGGC	1 cut(s)	707
MseI	TTAA	2 cut(s)	375, 660
MslI	CAYNNNNRTG	2 cut(s)	1086, 1427
MspA1I	CMGCKG	2 cut(s)	1052, 1355
MspI	CCGG	7 cut(s)	20, 413, 708, 986, 1002, 1167, 1596
MspR9I	CCNGG	1 cut(s)	951
Mva1269I	GAATGC	1 cut(s)	904
MvaI	CCWGG	1 cut(s)	951
MvnI	CGCG	3 cut(s)	725, 983, 1297
MwoI	GCNNNNNNNGC	7 cut(s)	534, 731, 1294, 1310, 1352, 1358, 1573
NaeI	GCCGGC	1 cut(s)	709
NarI	GGCGCC	1 cut(s)	543
NdeII	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
NgoMIV	GCCGGC	1 cut(s)	707
NlaIII	CATG	6 cut(s)	144, 472, 597, 715, 871, 1036
NlaIV	GGNNCC	8 cut(s)	544, 563, 817, 920, 961, 1061, 1062, 1728
NmeAIII	GCCGAG	1 cut(s)	1593
NmuCI	GTSAC	3 cut(s)	25, 1004, 1717
NspI	RCATGY	1 cut(s)	715
NspV	TTCGAA	1 cut(s)	1085
OliI	CACNNNNGTG	1 cut(s)	1427
PaeI	GCATGC	1 cut(s)	715
PaeR7I	CTCGAG	1 cut(s)	572
PagI	TCATGA	1 cut(s)	468
PciSI	GCTCTTC	1 cut(s)	605
PcsI	WCGNNNNNNNCGW	1 cut(s)	1707
PctI	GAATGC	1 cut(s)	904
PdiI	GCCGGC	1 cut(s)	709
PfeI	GAWTC	2 cut(s)	1170, 1503
PflFI	GACNNNGTC	1 cut(s)	554
PflMI	CCANNNNNTGG	1 cut(s)	1261
PinAI	ACCGGT	1 cut(s)	1001
PkrI	GCNGC	7 cut(s)	344, 351, 381, 517, 959, 1354, 1617
Ple19I	CGATCG	1 cut(s)	1096
PleI	GAGTC	5 cut(s)	692, 784, 1142, 1157, 1214
PluTI	GGCGCC	1 cut(s)	546
PpsI	GAGTC	5 cut(s)	692, 784, 1142, 1157, 1214
Ppu21I	YACGTR	2 cut(s)	131, 497
PpuMI	RGGWCCY	1 cut(s)	1060
PshAI	GACNNNNGTC	1 cut(s)	794
Psp124BI	GAGCTC	2 cut(s)	468, 579
Psp5II	RGGWCCY	1 cut(s)	1060
Psp6I	CCWGG	1 cut(s)	949
PspFI	CCCAGC	2 cut(s)	1014, 1336
PspGI	CCWGG	1 cut(s)	949
PspN4I	GGNNCC	8 cut(s)	544, 563, 817, 920, 961, 1061, 1062, 1728
PspPI	GGNCC	6 cut(s)	302, 475, 816, 999, 1060, 1574
PspPPI	RGGWCCY	1 cut(s)	1060
PspXI	VCTCGAGB	1 cut(s)	572
PsrI	GAACNNNNNNTAC	2 cut(s)	1126, 1158
PstI	CTGCAG	1 cut(s)	310
PstNI	CAGNNNCTG	2 cut(s)	1221, 1388
PsuI	RGATCY	1 cut(s)	642
PsyI	GACNNNGTC	1 cut(s)	554
PvuI	CGATCG	1 cut(s)	1096
PvuII	CAGCTG	1 cut(s)	1355
RsaI	GTAC	3 cut(s)	629, 872, 926
RsaNI	GTAC	3 cut(s)	628, 871, 925
RseI	CAYNNNNRTG	2 cut(s)	1086, 1427
SacI	GAGCTC	2 cut(s)	468, 579
SapI	GCTCTTC	1 cut(s)	605
SaqAI	TTAA	2 cut(s)	375, 660
SatI	GCNGC	7 cut(s)	343, 350, 380, 516, 958, 1353, 1616
Sau3AI	GATC	7 cut(s)	169, 642, 736, 767, 799, 1093, 1138
Sau96I	GGNCC	6 cut(s)	302, 475, 816, 999, 1060, 1574
SchI	GAGTC	5 cut(s)	692, 784, 1142, 1157, 1215
ScrFI	CCNGG	1 cut(s)	951
SduI	GDGCHC	5 cut(s)	248, 333, 468, 579, 1731
SfaNI	GCATC	1 cut(s)	411
SfcI	CTRYAG	3 cut(s)	273, 306, 436
SfoI	GGCGCC	1 cut(s)	544
Sfr274I	CTCGAG	1 cut(s)	572
SfuI	TTCGAA	1 cut(s)	1085
SinI	GGWCC	4 cut(s)	302, 816, 999, 1060
SlaI	CTCGAG	1 cut(s)	572
SmiMI	CAYNNNNRTG	2 cut(s)	1086, 1427
SmlI	CTYRAG	1 cut(s)	572
SmoI	CTYRAG	1 cut(s)	572
SphI	GCATGC	1 cut(s)	715
Sse9I	AATT	4 cut(s)	889, 1039, 1686, 1769
SspDI	GGCGCC	1 cut(s)	542
SspMI	CTAG	2 cut(s)	188, 647
SstI	GAGCTC	2 cut(s)	468, 579
StyD4I	CCNGG	1 cut(s)	949
StyI	CCWWGG	3 cut(s)	646, 1271, 1467
TaaI	ACNGT	4 cut(s)	40, 180, 617, 1426
TaiI	ACGT	6 cut(s)	8, 133, 291, 499, 1524, 1557
TaqII	GACCGA	2 cut(s)	833, 1453
TasI	AATT	4 cut(s)	889, 1039, 1686, 1769
TatI	WGTACW	2 cut(s)	627, 870
TauI	GCSGC	3 cut(s)	352, 960, 1618
TfiI	GAWTC	2 cut(s)	1170, 1503
Tru1I	TTAA	2 cut(s)	375, 660
Tru9I	TTAA	2 cut(s)	375, 660
TscAI	CASTG	3 cut(s)	333, 723, 1393
TseFI	GTSAC	3 cut(s)	25, 1004, 1717
TseI	GCWGC	4 cut(s)	342, 379, 515, 1352
Tsp45I	GTSAC	3 cut(s)	25, 1004, 1717
TspDTI	ATGAA	3 cut(s)	485, 980, 1658
TspGWI	ACGGA	1 cut(s)	1601
TspRI	CASTG	3 cut(s)	333, 723, 1393
Tth111I	GACNNNGTC	1 cut(s)	554
Van91I	CCANNNNNTGG	1 cut(s)	1261
VpaK11BI	GGWCC	4 cut(s)	302, 816, 999, 1060
XapI	RAATTY	2 cut(s)	1039, 1769
XceI	RCATGY	1 cut(s)	715
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1375
XhoI	CTCGAG	1 cut(s)	572
XmaJI	CCTAGG	1 cut(s)	646
XmiI	GTMKAC	1 cut(s)	1251
XspI	CTAG	2 cut(s)	188, 647
ZraI	GACGTC	2 cut(s)	6, 1555

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.