Rroxscaffold_7G00203160

Common central domain of tyrosinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000836
Physical Location & Seq
Forward (+)
51695033 .. 51696639
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00203160.1

Sequence Viewer

Length: 1083 bp
ATGCAACTGTTACTCTTTGTTTACAGAAAACTTATAGACACAATCCTAGGCCAATTAGGAATAATTGAGTCAGATGGGTCTTCAGGAAGCTATAAGATTGATAAAGAGGACCTCCCAGTGAGTCTCAACTTAACTAGTTGTCACTCAACTTATGGAAGGCCAGATCTTCTCGTCTGTTATTGCCCTCCAAGAAGTATAGCAGAAGAACCCGCCATTGATTTTCAATTTCCAGATCCTTCAACCTTTCTAAGAATTCGAAAGCCAGCACATCGTGACTCCCCTGATGGAATGGCAATGCTTGAATGGTACATGCATTCACCTTTTTTCGACAATCAGCGGGATGTTTCTCACTTCCCACCAAAAACGGTGTCTGGAGCAAAGAAGCCAGAGCTGTTCATGGGGTGCCCTTATAAGAATAGTAAGGAGGGTGTTTGTGATGGCCTTGGAACAATAAAGACTAGGGACCCTAGTTTCTATGCACACCACTCGAACGTGGACCGACTTTGGGAGCTTGTGAGAATCAAACTTCGTGATGTTTTGGATACTACAAAGTTGAGGTATGTTTATGAGGAGGTTCATCTTCCATGGCTAAATACGCGTCCAAAGCCTAATTCTATTCCATCCAAAATTGCCAACCATGCAATGAAAATAAGTGAAAATAGAACCATTTTGTCTGCAAAGGTAGGAGCAGGTGGTCGGAATCTTGAGAGCTCCATAACATTGAAGGTGGGCAGGTCTAAATTTAATAGAAGAAAGCAAGAGAAAGATGAGGAGGAAGAGGTTTTGGTTGTTTATGGAATTTCTGTGAGAATTGATGAGTATGTTAAGTTTGATGTGTATGTAAATGTAGTCGATGAGAGCAAAATAGGACCAGGTTTCAGGGAATTTGCCAGAACCTTTGTTCACATACCTCATGGAAATATGGATGGTGACAGAAAGACCAATCTGAAGTTTGGTATTTCAGAAGTGCTTGAGGATTTGGTGTCAGATAGAGATGAGAGCATCTGGGTCACATTGCTACTCAGGACTAAATCATGCACTGACACAACAATTGATGGGATTCGGATTGAAAACATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110
Pfam Domains
Protein Families
PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

360

Amino Acids

41.11

Weight (kDa)

7.14

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPO1_DWL PF12142 170 - 202 3.1e-12 Polyphenol oxidase middle domain
PPO1_KFDV PF12143 234 - 357 3.6e-33 Chloroplastic Polyphenol Oxidase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AarI CACCTGC 1 cut(s) 680
Acc36I ACCTGC 2 cut(s) 680, 723
AccB1I GGYRCC 1 cut(s) 402
AccII CGCG 1 cut(s) 598
AciI CCGC 2 cut(s) 210, 337
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 4 cut(s) 252, 740, 798, 884
AcuI CTGAAG 2 cut(s) 66, 968
AdeI CACNNNGTG 1 cut(s) 272
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 505
AflIII ACRYGT 1 cut(s) 596
AgsI TTSAA 5 cut(s) 224, 240, 302, 724, 1070
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 1 cut(s) 871
AjuI GAANNNNNNNTTGG 2 cut(s) 595, 627
AluBI AGCT 4 cut(s) 90, 391, 511, 711
AluI AGCT 4 cut(s) 90, 391, 511, 711
Alw21I GWGCWC 1 cut(s) 713
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 1 cut(s) 227
AoxI GGCC 3 cut(s) 49, 158, 439
ApoI RAATTY 4 cut(s) 252, 740, 798, 884
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AspA2I CCTAGG 1 cut(s) 46
AspS9I GGNCC 4 cut(s) 109, 463, 496, 869
AsuHPI GGTGA 2 cut(s) 309, 941
AsuII TTCGAA 1 cut(s) 256
AvaII GGWCC 4 cut(s) 109, 463, 496, 869
AvrII CCTAGG 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 407
BanI GGYRCC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 713
BccI CCATC 6 cut(s) 68, 278, 431, 628, 920, 1049
BcgI CGANNNNNNTGC 2 cut(s) 468, 502
BciT130I CCWGG 1 cut(s) 873
BciVI GTATCC 1 cut(s) 535
BcoDI GTCTC 1 cut(s) 128
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 4 cut(s) 47, 135, 459, 468
BfuAI ACCTGC 2 cut(s) 680, 723
BfuI GTATCC 1 cut(s) 535
BglII AGATCT 1 cut(s) 163
BlnI CCTAGG 1 cut(s) 46
Bme1390I CCNGG 1 cut(s) 873
Bme18I GGWCC 4 cut(s) 109, 463, 496, 869
BmgT120I GGNCC 4 cut(s) 109, 463, 496, 869
BmiI GGNNCC 3 cut(s) 404, 464, 465
BmrFI CCNGG 1 cut(s) 873
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 1 cut(s) 1011
BmuI ACTGGG 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 256
BpuEI CTTGAG 2 cut(s) 725, 992
BsaJI CCNNGG 3 cut(s) 46, 442, 584
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse1I ACTGG 1 cut(s) 116
Bse3DI GCAATG 3 cut(s) 300, 648, 1013
BseBI CCWGG 1 cut(s) 873
BseDI CCNNGG 3 cut(s) 46, 442, 584
BseGI GGATG 3 cut(s) 346, 620, 931
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMI GCAATG 3 cut(s) 300, 648, 1013
BseMII CTCAG 1 cut(s) 1036
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 2 cut(s) 584, 785
BseSI GKGCMC 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 598
BshFI GGCC 3 cut(s) 51, 160, 441
BshNI GGYRCC 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 713
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 1 cut(s) 505
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 476
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 3 cut(s) 51, 160, 441
Bsp119I TTCGAA 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 407, 713
Bsp143I GATC 2 cut(s) 163, 232
Bsp19I CCATGG 1 cut(s) 584
BspACI CCGC 2 cut(s) 210, 337
BspANI GGCC 3 cut(s) 51, 160, 441
BspCNI CTCAG 1 cut(s) 1035
BspFNI CGCG 1 cut(s) 598
BspLI GGNNCC 3 cut(s) 404, 464, 465
BspMI ACCTGC 2 cut(s) 680, 723
BspPI GGATC 1 cut(s) 227
BspT104I TTCGAA 1 cut(s) 256
BspT107I GGYRCC 1 cut(s) 402
BsrDI GCAATG 3 cut(s) 300, 648, 1013
BsrI ACTGG 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 46, 442, 584
BssMI GATC 2 cut(s) 163, 232
BssT1I CCWWGG 3 cut(s) 46, 442, 584
Bst2UI CCWGG 1 cut(s) 873
Bst4CI ACNGT 2 cut(s) 9, 367
Bst6I CTCTTC 1 cut(s) 771
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 2 cut(s) 248, 1022
BstDSI CCRYGG 1 cut(s) 584
BstF5I GGATG 3 cut(s) 346, 620, 931
BstFNI CGCG 1 cut(s) 598
BstKTI GATC 2 cut(s) 166, 235
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 2 cut(s) 163, 232
BstMWI GCNNNNNNNGC 3 cut(s) 595, 604, 638
BstNI CCWGG 1 cut(s) 873
BstNSI RCATGY 1 cut(s) 313
BstSCI CCNGG 1 cut(s) 871
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 1 cut(s) 598
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 2 cut(s) 163, 232
BstYI RGATCY 2 cut(s) 163, 232
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 3 cut(s) 51, 160, 441
BtgI CCRYGG 1 cut(s) 584
BtsCI GGATG 3 cut(s) 346, 620, 931
BtsIMutI CAGTG 2 cut(s) 123, 1038
BveI ACCTGC 2 cut(s) 680, 723
Cac8I GCNNGC 1 cut(s) 264
Cfr13I GGNCC 4 cut(s) 109, 463, 496, 869
CseI GACGC 1 cut(s) 587
CsiI ACCWGGT 1 cut(s) 871
Csp6I GTAC 1 cut(s) 307
CviAII CATG 7 cut(s) 310, 397, 585, 638, 914, 1035, 1075
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 2 cut(s) 248, 1022
DpnI GATC 2 cut(s) 165, 234
DpnII GATC 2 cut(s) 163, 232
DraIII CACNNNGTG 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 771
EarI CTCTTC 1 cut(s) 771
Ecl136II GAGCTC 1 cut(s) 711
Eco130I CCWWGG 3 cut(s) 46, 442, 584
Eco24I GRGCYC 1 cut(s) 713
Eco47I GGWCC 4 cut(s) 109, 463, 496, 869
Eco53kI GAGCTC 1 cut(s) 711
Eco57I CTGAAG 2 cut(s) 66, 968
EcoICRI GAGCTC 1 cut(s) 711
EcoO109I RGGNCCY 2 cut(s) 109, 463
EcoRI GAATTC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 871
EcoT14I CCWWGG 3 cut(s) 46, 442, 584
EcoT22I ATGCAT 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 713
ErhI CCWWGG 3 cut(s) 46, 442, 584
FaeI CATG 7 cut(s) 313, 400, 588, 641, 917, 1038, 1078
FaqI GGGAC 1 cut(s) 476
FatI CATG 7 cut(s) 309, 396, 584, 637, 913, 1034, 1074
FauI CCCGC 2 cut(s) 217, 330
FokI GGATG 3 cut(s) 353, 607, 938
FriOI GRGCYC 1 cut(s) 713
FspBI CTAG 4 cut(s) 47, 135, 459, 468
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 3 cut(s) 51, 160, 441
HgaI GACGC 1 cut(s) 587
Hin1II CATG 7 cut(s) 313, 400, 588, 641, 917, 1038, 1078
HinfI GANTC 6 cut(s) 68, 121, 275, 519, 700, 1060
HphI GGTGA 2 cut(s) 309, 941
Hpy166II GTNNAC 3 cut(s) 22, 496, 904
Hpy188I TCNGA 6 cut(s) 73, 699, 948, 964, 988, 1065
Hpy188III TCNNGA 7 cut(s) 84, 230, 272, 372, 530, 704, 1024
Hpy8I GTNNAC 3 cut(s) 22, 496, 904
HpyAV CCTTC 3 cut(s) 150, 246, 718
HpyCH4III ACNGT 2 cut(s) 9, 367
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 6 cut(s) 4, 313, 479, 641, 677, 1038
HpyF10VI GCNNNNNNNGC 3 cut(s) 595, 604, 638
HpyF3I CTNAG 2 cut(s) 248, 1022
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 7 cut(s) 313, 400, 588, 641, 917, 1038, 1078
KflI GGGWCCC 1 cut(s) 463
Kzo9I GATC 2 cut(s) 163, 232
LmnI GCTCC 4 cut(s) 374, 508, 686, 716
LweI GCATC 1 cut(s) 1011
MabI ACCWGGT 1 cut(s) 871
MaeI CTAG 4 cut(s) 47, 135, 459, 468
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 5 cut(s) 9, 140, 272, 929, 1009
MalI GATC 2 cut(s) 165, 234
MboI GATC 2 cut(s) 163, 232
MboII GAAGA 6 cut(s) 72, 158, 215, 572, 762, 788
MfeI CAATTG 1 cut(s) 1050
MflI RGATCY 2 cut(s) 163, 232
MhlI GDGCHC 2 cut(s) 407, 713
MluI ACGCGT 1 cut(s) 596
MlyI GAGTC 3 cut(s) 77, 130, 269
MmeI TCCRAC 1 cut(s) 677
Mph1103I ATGCAT 1 cut(s) 315
MseI TTAA 3 cut(s) 131, 744, 825
MspA1I CMGCKG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 873
MunI CAATTG 1 cut(s) 1050
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 1 cut(s) 873
MvnI CGCG 1 cut(s) 598
MwoI GCNNNNNNNGC 3 cut(s) 595, 604, 638
NcoI CCATGG 1 cut(s) 584
NdeII GATC 2 cut(s) 163, 232
NlaIII CATG 7 cut(s) 313, 400, 588, 641, 917, 1038, 1078
NlaIV GGNNCC 3 cut(s) 404, 464, 465
NmuCI GTSAC 4 cut(s) 140, 272, 929, 1009
NsiI ATGCAT 1 cut(s) 315
NspI RCATGY 1 cut(s) 313
NspV TTCGAA 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 680
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 3 cut(s) 519, 700, 1060
PleI GAGTC 3 cut(s) 76, 129, 269
PpsI GAGTC 3 cut(s) 76, 129, 269
PpuMI RGGWCCY 2 cut(s) 109, 463
PsiI TTATAA 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 713
Psp5II RGGWCCY 2 cut(s) 109, 463
Psp6I CCWGG 1 cut(s) 871
PspGI CCWGG 1 cut(s) 871
PspN4I GGNNCC 3 cut(s) 404, 464, 465
PspPI GGNCC 4 cut(s) 109, 463, 496, 869
PspPPI RGGWCCY 2 cut(s) 109, 463
PsuI RGATCY 2 cut(s) 163, 232
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
SacI GAGCTC 1 cut(s) 713
SaqAI TTAA 3 cut(s) 131, 744, 825
Sau3AI GATC 2 cut(s) 163, 232
Sau96I GGNCC 4 cut(s) 109, 463, 496, 869
SchI GAGTC 3 cut(s) 77, 130, 269
ScrFI CCNGG 1 cut(s) 873
SduI GDGCHC 2 cut(s) 407, 713
SexAI ACCWGGT 1 cut(s) 871
SfaNI GCATC 1 cut(s) 1011
SfuI TTCGAA 1 cut(s) 256
SinI GGWCC 4 cut(s) 109, 463, 496, 869
SmlI CTYRAG 2 cut(s) 704, 971
SmoI CTYRAG 2 cut(s) 704, 971
SpeI ACTAGT 1 cut(s) 134
SsiI CCGC 2 cut(s) 210, 337
SspMI CTAG 4 cut(s) 47, 135, 459, 468
SstI GAGCTC 1 cut(s) 713
StyD4I CCNGG 1 cut(s) 871
StyI CCWWGG 3 cut(s) 46, 442, 584
TaaI ACNGT 2 cut(s) 9, 367
TaiI ACGT 1 cut(s) 495
TaqI TCGA 4 cut(s) 256, 327, 488, 852
TaqII GACCGA 1 cut(s) 513
TfiI GAWTC 3 cut(s) 519, 700, 1060
Tru1I TTAA 3 cut(s) 131, 744, 825
Tru9I TTAA 3 cut(s) 131, 744, 825
TscAI CASTG 2 cut(s) 123, 1045
TseFI GTSAC 4 cut(s) 140, 272, 929, 1009
Tsp45I GTSAC 4 cut(s) 140, 272, 929, 1009
TspDTI ATGAA 3 cut(s) 385, 566, 659
TspRI CASTG 2 cut(s) 123, 1045
VpaK11BI GGWCC 4 cut(s) 109, 463, 496, 869
XapI RAATTY 4 cut(s) 252, 740, 798, 884
XceI RCATGY 1 cut(s) 313
XmaJI CCTAGG 1 cut(s) 46
XspI CTAG 4 cut(s) 47, 135, 459, 468
Zsp2I ATGCAT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.