Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Reverse (-)

4589540 .. 4592311

Gene Structure 2772 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG053500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGCTTCTCTCTCACCTCCACTAGTCACCACGACTACCATTTCCTCTACTACAACTTCCCTTTCTCCCTTCTCCCACAAAAACTCTCAGATTTCCTTAATCGGGAAACCTAGGCAGTGCATCCGTACTAGGGTTTCATGCAAAGCCGCAAATAGCGACCAAAATGATGCACAACCTTCTGTAGCTAAGTTCGACAGGAGAAATGTGCTTCTCGGTCTTGGAGGCCTATATGGGGTGGCCGGCCTTGGAAGCGATCCCTTCGCTTTTGCCAAGCCCGTCGCCCCACCTGACGTGTCAAAATGCGGGGCGGCAGACTTGCCTAATGGCGTAACACCGACAAATTGTTGCCCGCCAGCGGCCTCAAAGATCATCGACTTCAAGCTGCCCTCGCCAACCCCATTGCGTGTTAGGCCCGCGGCTCACGCCGTGGATGATGCCTACATTGCAAAATACACCAAGGCCATGGAGCTCATGAAAGCCCTCCCCGACGATGATCCCCGTAGTTTCAAGCAACAAGCCAACGTGCATTGTGCTTATTGTGATGGTGCTTATGACCAAGCCGGGTTTCCGGATCTTGAGCTCCAAATCCACAACTCATGGCTTTTCTTTCCCTTCCATAGATACTACTTGTACTTCTACGAGAGAATCTTGGGTAAGCTCATCAACGACCCGACATTTGCTTTGCCGTTCTGGAACTGGGACTCCCCTGCTGGCATGCAGTTGCCTGCCTTGTTCGCCAATCCCAAATCACCGCTTTACGATAAGTTCAGAGCCGCCTCTCACCAGCCGCCGACCCTGATCGACCTCGATTTCAACGGTACCGAGGACAAGACATCAAACACAACTCAAATCAACAGCAACCTAAGCATTATGTACCGTCAAATGGTGTCCAACGCCAAGAACGCTCAGCTCTTTTTTGGCAACCCCTACAGGGCTGGGGACGAGCCTGACCCCGGTGGTGGCTCTATCGAGGGAACCCCACACGGGCCAGTCCACTTGTGGACTGGTGACAGCACCCAGCCCAACTTTGAGGACATGGGAAACTTCTACTCCGCCGGTAGAGATCCTATTTTCTTCTCGCACCACTCCAATGTTGATAGGATGTGGAGTATATGGAAGACCCTAGCTCCCAAAAACAAAGACATCACCGACTCTGATTGGTTAGACTCCGGCTTCCTATTCTACGACGAGAATGCAAACATGGTACGCGTGAAGGTACGCGACTGCCTTGATTCCAAAAATCTCGGCTATGTGTATCAAGACGTGGAGATTCCATGGCTGAACTCCAAGCCAACGCCGAGAAGGTCAAAGGTGGCATTCAGCAATATAGCCAAGAAGCTCGGTGTGGCTAACGCCGCCACCTCATCAGCCAAGGTGGTGAAGATCACTGATTTTCCTATATCTTTGAACCAAAAAATTAGCGTGGCGGTGCCAAGGCCCAAGCAGAAGAAGAGGAGCAAGAAGCAGAAGGAAGACGAGGAGGAGATATTGGTGATCGAAGGAATCGAGTTCGATAGAGATGTGGCGGTGAAGTTTGATGTGTACATTAATGACGAGGATGACTTGCCGAGTGGTCCGGACAAGAGTGAGTTCGCCGGAAGCTTTGTTAGTGTGCCGCACAGGCATAAGCACAAGAAGAAGATCAACACTATTCTGAGGTTGGGCTTGACGGACTTACTGGAGGATTTGGACGCCGAGGATGATGAGACGGTGGTGGTGACTTTGATTCCAAGGTATGCGGCGGAGAAAGTCAAGATTGGTGGCATCAAGATTGAATTTGCTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

595

Amino Acids

66.01

Weight (kDa)

6.68

Isoelectric Point (pI)

43.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	167 - 374	1.4e-37	Common central domain of tyrosinase
PPO1_DWL	PF12142	381 - 432	4.2e-28	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	465 - 593	4.1e-54	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	818
AccB1I	GGYRCC	2 cut(s)	818, 1429
AccII	CGCG	3 cut(s)	416, 1209, 1221
AccIII	TCCGGA	2 cut(s)	568, 1576
AclWI	GGATC	4 cut(s)	248, 488, 579, 1058
AcoI	YGGCCR	1 cut(s)	237
AcsI	RAATTY	1 cut(s)	1775
AcyI	GRCGYC	1 cut(s)	1692
AdeI	CACNNNGTG	1 cut(s)	427
AfaI	GTAC	7 cut(s)	127, 632, 820, 875, 1206, 1218, 1544
AfiI	CCNNNNNNNGG	9 cut(s)	102, 130, 232, 355, 883, 931, 953, 959, 984
AflIII	ACRYGT	2 cut(s)	291, 1207
AgsI	TTSAA	5 cut(s)	379, 508, 814, 1408, 1775
AhlI	ACTAGT	1 cut(s)	22
AjiI	CACGTC	2 cut(s)	292, 1264
AjuI	GAANNNNNNNTTGG	2 cut(s)	549, 581
AloI	GAACNNNNNNTCC	2 cut(s)	686, 718
AluBI	AGCT	9 cut(s)	185, 382, 469, 580, 658, 910, 1127, 1339, 1602
AluI	AGCT	9 cut(s)	185, 382, 469, 580, 658, 910, 1127, 1339, 1602
Alw21I	GWGCWC	2 cut(s)	471, 582
Alw26I	GTCTC	1 cut(s)	1700
AlwI	GGATC	4 cut(s)	248, 488, 579, 1058
Aor13HI	TCCGGA	2 cut(s)	568, 1576
AoxI	GGCC	8 cut(s)	223, 237, 241, 357, 410, 459, 986, 1436
ApeKI	GCWGC	1 cut(s)	382
ApoI	RAATTY	1 cut(s)	1775
ArsI	GACNNNNNNTTYG	4 cut(s)	659, 664, 691, 696
AseI	ATTAAT	1 cut(s)	1548
Asp718I	GGTACC	1 cut(s)	818
AspA2I	CCTAGG	1 cut(s)	110
AspS9I	GGNCC	4 cut(s)	411, 986, 1437, 1574
AsuC2I	CCSGG	2 cut(s)	562, 954
AvaII	GGWCC	1 cut(s)	1574
AvrII	CCTAGG	1 cut(s)	110
BanI	GGYRCC	2 cut(s)	818, 1429
BanII	GRGCYC	2 cut(s)	471, 582
BbsI	GAAGAC	2 cut(s)	1124, 1479
Bbv12I	GWGCWC	2 cut(s)	471, 582
BbvI	GCAGC	1 cut(s)	369
BccI	CCATC	1 cut(s)	536
BceAI	ACGGC	2 cut(s)	410, 670
BcgI	CGANNNNNNTGC	2 cut(s)	1175, 1209
BcnI	CCSGG	2 cut(s)	562, 954
BcoDI	GTCTC	1 cut(s)	1700
BcuI	ACTAGT	1 cut(s)	22
BfaI	CTAG	4 cut(s)	23, 111, 129, 1124
BfmI	CTRYAG	2 cut(s)	180, 928
BglI	GCCNNNNNGGC	1 cut(s)	1621
BlnI	CCTAGG	1 cut(s)	110
BlpI	GCTNAGC	1 cut(s)	906
Bme1390I	CCNGG	2 cut(s)	562, 954
Bme18I	GGWCC	1 cut(s)	1574
BmgBI	CACGTC	2 cut(s)	292, 1264
BmgT120I	GGNCC	4 cut(s)	411, 986, 1437, 1574
BmiI	GGNNCC	3 cut(s)	820, 976, 1431
BmrFI	CCNGG	2 cut(s)	562, 954
BmrI	ACTGGG	1 cut(s)	706
BmsI	GCATC	4 cut(s)	129, 157, 424, 1773
BmuI	ACTGGG	1 cut(s)	706
BpiI	GAAGAC	2 cut(s)	1124, 1479
BpmI	CTGGAG	1 cut(s)	1700
Bpu10I	CCTNAGC	1 cut(s)	863
Bpu1102I	GCTNAGC	1 cut(s)	906
BpuEI	CTTGAG	1 cut(s)	596
BpuMI	CCSGG	2 cut(s)	562, 954
BsaHI	GRCGYC	1 cut(s)	1692
BsaWI	WCCGGW	2 cut(s)	568, 1576
BsaXI	ACNNNNNCTCC	6 cut(s)	686, 716, 1034, 1064, 1111, 1141
Bsc4I	CCNNNNNNNGG	9 cut(s)	102, 130, 232, 355, 883, 931, 953, 959, 984
Bse118I	RCCGGY	2 cut(s)	239, 1055
Bse1I	ACTGG	4 cut(s)	701, 989, 1009, 1683
Bse3DI	GCAATG	2 cut(s)	398, 441
BseAI	TCCGGA	2 cut(s)	568, 1576
BseGI	GGATG	5 cut(s)	120, 436, 1107, 1564, 1705
BseLI	CCNNNNNNNGG	9 cut(s)	102, 130, 232, 355, 883, 931, 953, 959, 984
BseMI	GCAATG	2 cut(s)	398, 441
BseMII	CTCAG	3 cut(s)	101, 920, 1646
BseNI	ACTGG	4 cut(s)	701, 989, 1009, 1683
BseRI	GAGGAG	3 cut(s)	1468, 1493, 1496
BseXI	GCAGC	1 cut(s)	369
BseYI	CCCAGC	2 cut(s)	935, 1017
Bsh1236I	CGCG	3 cut(s)	416, 1209, 1221
BshFI	GGCC	8 cut(s)	225, 239, 243, 359, 412, 461, 988, 1438
BshNI	GGYRCC	2 cut(s)	818, 1429
BsiHKAI	GWGCWC	2 cut(s)	471, 582
BsiSI	CCGG	8 cut(s)	240, 561, 569, 954, 1056, 1170, 1577, 1596
BslFI	GGGAC	2 cut(s)	713, 953
BslI	CCNNNNNNNGG	9 cut(s)	102, 130, 232, 355, 883, 931, 953, 959, 984
BsmAI	GTCTC	1 cut(s)	1700
BsmBI	CGTCTC	1 cut(s)	1700
BsmFI	GGGAC	2 cut(s)	713, 953
BsmI	GAATGC	2 cut(s)	1198, 1316
BsnI	GGCC	8 cut(s)	225, 239, 243, 359, 412, 461, 988, 1438
Bsp1286I	GDGCHC	2 cut(s)	471, 582
Bsp13I	TCCGGA	2 cut(s)	568, 1576
Bsp1407I	TGTACA	1 cut(s)	1542
Bsp143I	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
Bsp1720I	GCTNAGC	1 cut(s)	906
Bsp19I	CCATGG	2 cut(s)	462, 1274
BspANI	GGCC	8 cut(s)	225, 239, 243, 359, 412, 461, 988, 1438
BspCNI	CTCAG	3 cut(s)	100, 919, 1647
BspEI	TCCGGA	2 cut(s)	568, 1576
BspFNI	CGCG	3 cut(s)	416, 1209, 1221
BspHI	TCATGA	1 cut(s)	471
BspLI	GGNNCC	3 cut(s)	820, 976, 1431
BspPI	GGATC	4 cut(s)	248, 488, 579, 1058
BspT107I	GGYRCC	2 cut(s)	818, 1429
BsrDI	GCAATG	2 cut(s)	398, 441
BsrFI	RCCGGY	2 cut(s)	239, 1055
BsrGI	TGTACA	1 cut(s)	1542
BsrI	ACTGG	4 cut(s)	701, 989, 1009, 1683
BssAI	RCCGGY	2 cut(s)	239, 1055
BssMI	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
BssNI	GRCGYC	1 cut(s)	1692
BssT1I	CCWWGG	8 cut(s)	110, 244, 456, 462, 1274, 1371, 1433, 1730
Bst4CI	ACNGT	3 cut(s)	818, 878, 1711
Bst6I	CTCTTC	1 cut(s)	1445
BstACI	GRCGYC	1 cut(s)	1692
BstAUI	TGTACA	1 cut(s)	1542
BstC8I	GCNNGC	7 cut(s)	241, 350, 354, 414, 712, 716, 726
BstDEI	CTNAG	5 cut(s)	87, 186, 863, 906, 1655
BstDSI	CCRYGG	4 cut(s)	414, 426, 462, 1274
BstF5I	GGATG	5 cut(s)	120, 436, 1107, 1564, 1705
BstFNI	CGCG	3 cut(s)	416, 1209, 1221
BstKTI	GATC	9 cut(s)	256, 369, 496, 574, 801, 1066, 1386, 1497, 1644
BstMAI	GTCTC	1 cut(s)	1700
BstMBI	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
BstNSI	RCATGY	1 cut(s)	718
BstSCI	CCNGG	2 cut(s)	560, 952
BstSFI	CTRYAG	2 cut(s)	180, 928
BstUI	CGCG	3 cut(s)	416, 1209, 1221
BstV1I	GCAGC	1 cut(s)	369
BstV2I	GAAGAC	2 cut(s)	1124, 1479
BstX2I	RGATCY	2 cut(s)	571, 1063
BstXI	CCANNNNNNTGG	1 cut(s)	463
BstYI	RGATCY	2 cut(s)	571, 1063
BsuRI	GGCC	8 cut(s)	225, 239, 243, 359, 412, 461, 988, 1438
BtgI	CCRYGG	4 cut(s)	414, 426, 462, 1274
BtrI	CACGTC	2 cut(s)	292, 1264
BtsCI	GGATG	5 cut(s)	120, 436, 1107, 1564, 1705
BtsI	GCAGTG	1 cut(s)	122
BtsIMutI	CAGTG	2 cut(s)	122, 1386
Cac8I	GCNNGC	7 cut(s)	241, 350, 354, 414, 712, 716, 726
CciI	TCATGA	1 cut(s)	471
Cfr10I	RCCGGY	2 cut(s)	239, 1055
Cfr13I	GGNCC	4 cut(s)	411, 986, 1437, 1574
Cfr42I	CCGCGG	1 cut(s)	417
CseI	GACGC	1 cut(s)	1700
Csp6I	GTAC	7 cut(s)	126, 631, 819, 874, 1205, 1217, 1543
CspCI	CAANNNNNGTGG	2 cut(s)	1741, 1776
CviAII	CATG	8 cut(s)	138, 463, 472, 597, 715, 1036, 1201, 1275
CviQI	GTAC	7 cut(s)	126, 631, 819, 874, 1205, 1217, 1543
DdeI	CTNAG	5 cut(s)	87, 186, 863, 906, 1655
DpnI	GATC	9 cut(s)	255, 368, 495, 573, 800, 1065, 1385, 1496, 1643
DpnII	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
DraIII	CACNNNGTG	1 cut(s)	427
EaeI	YGGCCR	1 cut(s)	237
Eam1104I	CTCTTC	1 cut(s)	1445
EarI	CTCTTC	1 cut(s)	1445
EciI	GGCGGA	2 cut(s)	1042, 1757
Ecl136II	GAGCTC	2 cut(s)	469, 580
Eco130I	CCWWGG	8 cut(s)	110, 244, 456, 462, 1274, 1371, 1433, 1730
Eco147I	AGGCCT	1 cut(s)	225
Eco24I	GRGCYC	2 cut(s)	471, 582
Eco47I	GGWCC	1 cut(s)	1574
Eco53kI	GAGCTC	2 cut(s)	469, 580
EcoICRI	GAGCTC	2 cut(s)	469, 580
EcoT14I	CCWWGG	8 cut(s)	110, 244, 456, 462, 1274, 1371, 1433, 1730
EcoT38I	GRGCYC	2 cut(s)	471, 582
ErhI	CCWWGG	8 cut(s)	110, 244, 456, 462, 1274, 1371, 1433, 1730
Esp3I	CGTCTC	1 cut(s)	1700
FaeI	CATG	8 cut(s)	141, 466, 475, 600, 718, 1039, 1204, 1278
FaqI	GGGAC	2 cut(s)	713, 953
FatI	CATG	8 cut(s)	137, 462, 471, 596, 714, 1035, 1200, 1274
FauI	CCCGC	3 cut(s)	296, 357, 421
FokI	GGATG	5 cut(s)	107, 443, 1114, 1571, 1712
FriOI	GRGCYC	2 cut(s)	471, 582
FseI	GGCCGGCC	1 cut(s)	243
FspBI	CTAG	4 cut(s)	23, 111, 129, 1124
GsaI	CCCAGC	2 cut(s)	939, 1021
GsuI	CTGGAG	1 cut(s)	1700
HaeIII	GGCC	8 cut(s)	225, 239, 243, 359, 412, 461, 988, 1438
HapII	CCGG	8 cut(s)	240, 561, 569, 954, 1056, 1170, 1577, 1596
HgaI	GACGC	1 cut(s)	1700
Hin1I	GRCGYC	1 cut(s)	1692
Hin1II	CATG	8 cut(s)	141, 466, 475, 600, 718, 1039, 1204, 1278
HindIII	AAGCTT	1 cut(s)	1600
HinfI	GANTC	8 cut(s)	645, 701, 1151, 1166, 1232, 1270, 1503, 1726
HpaII	CCGG	8 cut(s)	240, 561, 569, 954, 1056, 1170, 1577, 1596
Hpy166II	GTNNAC	3 cut(s)	994, 1002, 1543
Hpy188I	TCNGA	4 cut(s)	90, 770, 1156, 1656
Hpy188III	TCNNGA	9 cut(s)	103, 472, 569, 575, 691, 1259, 1577, 1753, 1768
Hpy8I	GTNNAC	3 cut(s)	994, 1002, 1543
Hpy99I	CGWCG	3 cut(s)	281, 491, 1190
HpyAV	CCTTC	8 cut(s)	79, 186, 268, 622, 1207, 1296, 1462, 1493
HpyCH4III	ACNGT	3 cut(s)	818, 878, 1711
HpyCH4IV	ACGT	3 cut(s)	291, 522, 1263
HpyCH4V	TGCA	7 cut(s)	120, 141, 170, 446, 526, 718, 1196
HpyF3I	CTNAG	5 cut(s)	87, 186, 863, 906, 1655
HpySE526I	ACGT	3 cut(s)	291, 522, 1263
Hsp92I	GRCGYC	1 cut(s)	1692
Hsp92II	CATG	8 cut(s)	141, 466, 475, 600, 718, 1039, 1204, 1278
Kpn2I	TCCGGA	2 cut(s)	568, 1576
KpnI	GGTACC	1 cut(s)	822
KroI	GCCGGC	1 cut(s)	239
KroNI	GCCGGC	1 cut(s)	241
KspI	CCGCGG	1 cut(s)	417
Kzo9I	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
LmnI	GCTCC	4 cut(s)	466, 585, 1132, 1455
Lsp1109I	GCAGC	1 cut(s)	369
LweI	GCATC	4 cut(s)	129, 157, 424, 1773
MaeI	CTAG	4 cut(s)	23, 111, 129, 1124
MaeII	ACGT	3 cut(s)	291, 522, 1263
MaeIII	GTNAC	4 cut(s)	25, 328, 1007, 1717
MalI	GATC	9 cut(s)	255, 368, 495, 573, 800, 1065, 1385, 1496, 1643
MboI	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
MboII	GAAGA	8 cut(s)	1066, 1129, 1393, 1459, 1462, 1484, 1648, 1651
MflI	RGATCY	2 cut(s)	571, 1063
MhlI	GDGCHC	2 cut(s)	471, 582
MluCI	AATT	3 cut(s)	340, 1416, 1775
MluI	ACGCGT	1 cut(s)	1207
MlyI	GAGTC	3 cut(s)	695, 1145, 1160
MmeI	TCCRAC	1 cut(s)	915
MroI	TCCGGA	2 cut(s)	568, 1576
MroNI	GCCGGC	1 cut(s)	239
MseI	TTAA	3 cut(s)	98, 1548, 1786
MslI	CAYNNNNRTG	1 cut(s)	1089
MspA1I	CMGCKG	2 cut(s)	356, 416
MspI	CCGG	8 cut(s)	240, 561, 569, 954, 1056, 1170, 1577, 1596
MspR9I	CCNGG	2 cut(s)	562, 954
Mva1269I	GAATGC	2 cut(s)	1198, 1316
MvnI	CGCG	3 cut(s)	416, 1209, 1221
NaeI	GCCGGC	1 cut(s)	241
NciI	CCSGG	2 cut(s)	562, 954
NcoI	CCATGG	2 cut(s)	462, 1274
NdeII	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
NgoMIV	GCCGGC	1 cut(s)	239
NlaIII	CATG	8 cut(s)	141, 466, 475, 600, 718, 1039, 1204, 1278
NlaIV	GGNNCC	3 cut(s)	820, 976, 1431
NmeAIII	GCCGAG	4 cut(s)	1224, 1323, 1593, 1720
NmuCI	GTSAC	3 cut(s)	25, 1007, 1717
NspI	RCATGY	1 cut(s)	718
PaeI	GCATGC	1 cut(s)	718
PagI	TCATGA	1 cut(s)	471
PceI	AGGCCT	1 cut(s)	225
PcsI	WCGNNNNNNNCGW	1 cut(s)	1503
PctI	GAATGC	2 cut(s)	1198, 1316
PdiI	GCCGGC	1 cut(s)	241
PfeI	GAWTC	5 cut(s)	645, 1232, 1270, 1503, 1726
PleI	GAGTC	3 cut(s)	695, 1145, 1160
PpsI	GAGTC	3 cut(s)	695, 1145, 1160
PshBI	ATTAAT	1 cut(s)	1548
Psp124BI	GAGCTC	2 cut(s)	471, 582
PspFI	CCCAGC	2 cut(s)	935, 1017
PspN4I	GGNNCC	3 cut(s)	820, 976, 1431
PspPI	GGNCC	4 cut(s)	411, 986, 1437, 1574
PsuI	RGATCY	2 cut(s)	571, 1063
RigI	GGCCGGCC	1 cut(s)	243
RsaI	GTAC	7 cut(s)	127, 632, 820, 875, 1206, 1218, 1544
RsaNI	GTAC	7 cut(s)	126, 631, 819, 874, 1205, 1217, 1543
RseI	CAYNNNNRTG	1 cut(s)	1089
SacI	GAGCTC	2 cut(s)	471, 582
SacII	CCGCGG	1 cut(s)	417
SaqAI	TTAA	3 cut(s)	98, 1548, 1786
Sau3AI	GATC	9 cut(s)	253, 366, 493, 571, 798, 1063, 1383, 1494, 1641
Sau96I	GGNCC	4 cut(s)	411, 986, 1437, 1574
SchI	GAGTC	3 cut(s)	695, 1145, 1160
ScrFI	CCNGG	2 cut(s)	562, 954
SduI	GDGCHC	2 cut(s)	471, 582
SfaNI	GCATC	4 cut(s)	129, 157, 424, 1773
SfcI	CTRYAG	2 cut(s)	180, 928
Sfr303I	CCGCGG	1 cut(s)	417
SgrBI	CCGCGG	1 cut(s)	417
SinI	GGWCC	1 cut(s)	1574
SmiMI	CAYNNNNRTG	1 cut(s)	1089
SmlI	CTYRAG	1 cut(s)	575
SmoI	CTYRAG	1 cut(s)	575
SpeI	ACTAGT	1 cut(s)	22
SphI	GCATGC	1 cut(s)	718
Sse9I	AATT	3 cut(s)	340, 1416, 1775
SseBI	AGGCCT	1 cut(s)	225
SspMI	CTAG	4 cut(s)	23, 111, 129, 1124
SstI	GAGCTC	2 cut(s)	471, 582
StuI	AGGCCT	1 cut(s)	225
StyD4I	CCNGG	2 cut(s)	560, 952
StyI	CCWWGG	8 cut(s)	110, 244, 456, 462, 1274, 1371, 1433, 1730
TaaI	ACNGT	3 cut(s)	818, 878, 1711
TaiI	ACGT	3 cut(s)	294, 525, 1266
TaqI	TCGA	8 cut(s)	192, 372, 801, 807, 969, 1497, 1506, 1512
TaqII	GACCGA	1 cut(s)	203
TasI	AATT	3 cut(s)	340, 1416, 1775
TatI	WGTACW	2 cut(s)	630, 1542
TauI	GCSGC	9 cut(s)	149, 311, 359, 419, 776, 790, 1358, 1618, 1742
TfiI	GAWTC	5 cut(s)	645, 1232, 1270, 1503, 1726
Tru1I	TTAA	3 cut(s)	98, 1548, 1786
Tru9I	TTAA	3 cut(s)	98, 1548, 1786
TscAI	CASTG	2 cut(s)	122, 1393
TseFI	GTSAC	3 cut(s)	25, 1007, 1717
TseI	GCWGC	1 cut(s)	382
Tsp45I	GTSAC	3 cut(s)	25, 1007, 1717
TspDTI	ATGAA	2 cut(s)	126, 488
TspGWI	ACGGA	2 cut(s)	113, 1685
TspRI	CASTG	2 cut(s)	122, 1393
VpaK11BI	GGWCC	1 cut(s)	1574
VspI	ATTAAT	1 cut(s)	1548
XapI	RAATTY	1 cut(s)	1775
XceI	RCATGY	1 cut(s)	718
XcmI	CCANNNNNNNNNTGG	2 cut(s)	996, 1001
XmaJI	CCTAGG	1 cut(s)	110
XspI	CTAG	4 cut(s)	23, 111, 129, 1124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.