Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Forward (+)

44686442 .. 44688569

Gene Structure 2128 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1319100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGCTACTGCACCATCTCCCACTGGTTCTACCACCACAATGGGCACTTACTCTTCTCTGATTATCTCCACCAATTCCTACTCCGCGTTCCTCCCAAACAAATCCCAACTTTCCTTCTCTGGAAAAAGAAAGCACTACATTTCCCGTAGATCATCAATCTCGTGCAAAGCCACAAACCCTAATAATAATGAGAAACAGAACCAACCAGAGCAATCATCTAATTTGTTGGGAAAGTTGGACAGGAGAAATGTCCTAATTGGCCTCGGCGGCCTCTACGGCGCCACCACCCTTGCCCAGAAACCATTGGCCTTCGCCACCCCGCTGGCCCCACCTGACCTAACCAAGTGCACACTGGCCGAAATCACCACCGGAGGTGAAACTGTGGCATGCTGTCCACCGGTCTCCACAAAGATCAAAACCTTCACTCTGGACCAGTCTATCCCACTGCGGACGAGGCCTGCCGCCCATCTGGTCACGGACGAGTACTTGGCCAAGTTCAAGAAAGCTCAAGCCCTCATGCGTGCCTTACCCGAAGACGACCCGCGTAGCATGGTTCAACAAGCCAAAGTTCACTGTGCTTACTGCAATGGTGCTTATCCACAAGTAGGGTTTCCGGACATTGACATCCAAGTCCATTTCTCCTGGCTTTTCTTTCCCTTCCACCGCATGTACTTGTATTTCTACGAGAGAATCCTTGGCAAGCTCATTGATGACCCAACTTTTGCTCTCCCATACTGGAATTGGGACTCTCCCGATGGCTTTCCAATTCCCGACATTTATACTGACACCACCTCCCCACTTTACGATCAGTACCGTCACGCCGGACACCAGCCCCCTGTGCTGGTGGACCTCAGCTACGGTGGAACCGATGATGACGTGGACGACCAGACAAGAATAGATGAGAATCTAGCCATCATGTACCGGCAAATGGTTTCCGGTGCCAAAACACCGGATCTATTTTTCGGCCACGAATACAGGGCAGGGGAAACAACGACAGGGAAATACGCTGGCACCATTGAGAACAATCCTCACAATAACATCCATCTTTGGTGCGGTGACCCGAACCAGCCCAACAAGGAGGACATGGGCAACTTCTACTCAGCCGGTCGGGATCCTCTGTTTTACGCCCACCATTGCAACGTGGACCGCATGTGGAACATTTGGAAAACCCTCGGAGGCAAGCGCAAGGACCCTACCGACACCGATTGGCTCGATGCTGAGTTTCTGTTCTACGACGAAAACGCCGAGCTTGTGAGCTGTAAGGTTCGGGACAGCATCCATCCTGCGAAAGATCTTCGCTATACTTACGAGCCTGTTAGTGTTCCGTGGCTGTTCTCGAAGCCAACAGCTCGTAAGCCGAGGAACAAAACCAAAGCCAAGGTGTCGGCTACTCAGCTGACAACCAAGTTCCCTGCAACGTTTGACTCGAAGACGACAGTGGAGGTAGCGAGGCCAAAGCCGAGGAAGAGGACCAAGAAGGAGAAGAGCAACGAGGAGGAGGTGCTGATCATCAAGGATATTGAGTTTGAAAGCAACGAGGCTGTGAAGTTCGATGTGTTTATTAATGACGATGCTGAGTCGCTGAGTCGAAAGGACAAGTCAGAATTTGCCGGTAGTTTTGTGCACGTGCCGCATAACCAGAAGACTGGTACGAAGACGAAGACGAATCTGAAACTGGGGATCACGGACTTATTGGAGGATTTGGGTGTGGAGGATGATAGCAGTGTGCTGGTGACGTTGGTACCTAGGGTTTCCAACTCGCCAATCACCGTTGGTGGGTTTAAGATCGAGTATTCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

601

Amino Acids

67.38

Weight (kDa)

6.74

Isoelectric Point (pI)

44.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	182 - 390	2.7e-38	Common central domain of tyrosinase
PPO1_DWL	PF12142	397 - 449	2.5e-20	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	470 - 598	2.8e-49	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	629
Acc65I	GGTACC	1 cut(s)	1741
AccB1I	GGYRCC	4 cut(s)	278, 938, 1010, 1741
AccII	CGCG	2 cut(s)	86, 544
AccIII	TCCGGA	1 cut(s)	613
AclI	AACGTT	1 cut(s)	1418
AclWI	GGATC	4 cut(s)	960, 1106, 1119, 1688
AcoI	YGGCCR	3 cut(s)	354, 489, 964
AcsI	RAATTY	1 cut(s)	1604
AcvI	CACGTG	1 cut(s)	1627
AcyI	GRCGYC	1 cut(s)	279
AfaI	GTAC	6 cut(s)	485, 671, 812, 920, 1651, 1743
AfiI	CCNNNNNNNGG	4 cut(s)	605, 841, 928, 1776
AgeI	ACCGGT	1 cut(s)	397
AgsI	TTSAA	3 cut(s)	499, 557, 1529
AjiI	CACGTC	1 cut(s)	877
AjnI	CCWGG	1 cut(s)	641
AluBI	AGCT	7 cut(s)	506, 703, 855, 1249, 1257, 1349, 1396
AluI	AGCT	7 cut(s)	506, 703, 855, 1249, 1257, 1349, 1396
Alw21I	GWGCWC	2 cut(s)	350, 1626
Alw26I	GTCTC	1 cut(s)	406
Alw44I	GTGCAC	2 cut(s)	346, 1622
AlwI	GGATC	4 cut(s)	960, 1106, 1119, 1688
Aor13HI	TCCGGA	1 cut(s)	613
AoxI	GGCC	9 cut(s)	259, 268, 306, 324, 354, 455, 489, 964, 1451
ApaLI	GTGCAC	2 cut(s)	346, 1622
ApoI	RAATTY	1 cut(s)	1604
ArsI	GACNNNNNNTTYG	2 cut(s)	704, 736
AseI	ATTAAT	1 cut(s)	1563
AsiGI	ACCGGT	1 cut(s)	397
Asp718I	GGTACC	1 cut(s)	1741
AspA2I	CCTAGG	1 cut(s)	1745
AspLEI	GCGC	2 cut(s)	281, 1185
AspS9I	GGNCC	6 cut(s)	325, 430, 847, 1144, 1189, 1470
AsuHPI	GGTGA	5 cut(s)	355, 386, 1067, 1744, 1759
AvaII	GGWCC	5 cut(s)	430, 847, 1144, 1189, 1470
AvrII	CCTAGG	1 cut(s)	1745
BaeGI	GKGCMC	3 cut(s)	47, 350, 1626
BaeI	ACNNNNGTAYC	2 cut(s)	1725, 1758
BalI	TGGCCA	1 cut(s)	491
BamHI	GGATCC	1 cut(s)	1111
BanI	GGYRCC	4 cut(s)	278, 938, 1010, 1741
BauI	CACGAG	1 cut(s)	160
BbrPI	CACGTG	1 cut(s)	1627
BbsI	GAAGAC	5 cut(s)	540, 1436, 1649, 1661, 1667
Bbv12I	GWGCWC	2 cut(s)	350, 1626
BbvCI	CCTCAGC	1 cut(s)	851
BccI	CCATC	6 cut(s)	22, 474, 749, 920, 1050, 1287
BceAI	ACGGC	1 cut(s)	292
BciT130I	CCWGG	1 cut(s)	643
BclI	TGATCA	1 cut(s)	1506
BcoDI	GTCTC	1 cut(s)	406
BfaI	CTAG	2 cut(s)	908, 1746
BfoI	RGCGCY	1 cut(s)	282
BglI	GCCNNNNNGGC	2 cut(s)	267, 276
BglII	AGATCT	1 cut(s)	1291
BisI	GCNGC	3 cut(s)	268, 462, 1631
BlnI	CCTAGG	1 cut(s)	1745
BlsI	GCNGC	3 cut(s)	269, 463, 1632
BmcAI	AGTACT	1 cut(s)	485
Bme1390I	CCNGG	1 cut(s)	643
Bme18I	GGWCC	5 cut(s)	430, 847, 1144, 1189, 1470
BmgBI	CACGTC	1 cut(s)	877
BmgT120I	GGNCC	6 cut(s)	325, 430, 847, 1144, 1189, 1470
BmiI	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
BmrFI	CCNGG	1 cut(s)	643
BmrI	ACTGGG	1 cut(s)	1685
BmsI	GCATC	3 cut(s)	1204, 1284, 1561
BmuI	ACTGGG	1 cut(s)	1685
BpiI	GAAGAC	5 cut(s)	540, 1436, 1649, 1661, 1667
Bpu10I	CCTNAGC	1 cut(s)	851
BpuEI	CTTGAG	1 cut(s)	492
BsaAI	YACGTR	1 cut(s)	1627
BsaBI	GATNNNNATC	1 cut(s)	903
BsaHI	GRCGYC	1 cut(s)	279
BsaI	GGTCTC	1 cut(s)	406
BsaJI	CCNNGG	8 cut(s)	262, 694, 1171, 1325, 1358, 1377, 1460, 1745
BsaWI	WCCGGW	5 cut(s)	368, 397, 613, 935, 949
BsaXI	ACNNNNNCTCC	2 cut(s)	776, 806
Bsc4I	CCNNNNNNNGG	4 cut(s)	605, 841, 928, 1776
Bse118I	RCCGGY	4 cut(s)	397, 921, 1103, 1610
Bse1I	ACTGG	6 cut(s)	28, 357, 433, 740, 1651, 1680
Bse3DI	GCAATG	2 cut(s)	592, 1132
Bse8I	GATNNNNATC	1 cut(s)	903
BseAI	TCCGGA	1 cut(s)	613
BseBI	CCWGG	1 cut(s)	643
BseDI	CCNNGG	8 cut(s)	262, 694, 1171, 1325, 1358, 1377, 1460, 1745
BseGI	GGATG	5 cut(s)	624, 1038, 1275, 1279, 1720
BseJI	GATNNNNATC	1 cut(s)	903
BseLI	CCNNNNNNNGG	4 cut(s)	605, 841, 928, 1776
BseMI	GCAATG	2 cut(s)	592, 1132
BseMII	CTCAG	6 cut(s)	865, 1113, 1209, 1406, 1566, 1574
BseNI	ACTGG	6 cut(s)	28, 357, 433, 740, 1651, 1680
BseRI	GAGGAG	2 cut(s)	1508, 1511
BseSI	GKGCMC	3 cut(s)	47, 350, 1626
Bsh1236I	CGCG	2 cut(s)	86, 544
Bsh1285I	CGRYCG	1 cut(s)	1108
BshFI	GGCC	9 cut(s)	261, 270, 308, 326, 356, 457, 491, 966, 1453
BshNI	GGYRCC	4 cut(s)	278, 938, 1010, 1741
BshTI	ACCGGT	1 cut(s)	397
BsiEI	CGRYCG	1 cut(s)	1108
BsiHKAI	GWGCWC	2 cut(s)	350, 1626
BsiSI	CCGG	9 cut(s)	369, 398, 614, 822, 922, 936, 950, 1104, 1611
BslFI	GGGAC	2 cut(s)	758, 1283
BslI	CCNNNNNNNGG	4 cut(s)	605, 841, 928, 1776
BsmAI	GTCTC	1 cut(s)	406
BsmFI	GGGAC	2 cut(s)	758, 1283
BsnI	GGCC	9 cut(s)	261, 270, 308, 326, 356, 457, 491, 966, 1453
Bso31I	GGTCTC	1 cut(s)	406
Bsp1286I	GDGCHC	3 cut(s)	47, 350, 1626
Bsp13I	TCCGGA	1 cut(s)	613
Bsp143I	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
BspANI	GGCC	9 cut(s)	261, 270, 308, 326, 356, 457, 491, 966, 1453
BspCNI	CTCAG	6 cut(s)	864, 1112, 1210, 1405, 1567, 1575
BspEI	TCCGGA	1 cut(s)	613
BspFNI	CGCG	2 cut(s)	86, 544
BspLI	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
BspPI	GGATC	4 cut(s)	960, 1106, 1119, 1688
BspQI	GCTCTTC	1 cut(s)	1478
BspT107I	GGYRCC	4 cut(s)	278, 938, 1010, 1741
BspTNI	GGTCTC	1 cut(s)	406
BsrDI	GCAATG	2 cut(s)	592, 1132
BsrFI	RCCGGY	4 cut(s)	397, 921, 1103, 1610
BsrI	ACTGG	6 cut(s)	28, 357, 433, 740, 1651, 1680
BssAI	RCCGGY	4 cut(s)	397, 921, 1103, 1610
BssECI	CCNNGG	8 cut(s)	262, 694, 1171, 1325, 1358, 1377, 1460, 1745
BssMI	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
BssNI	GRCGYC	1 cut(s)	279
BssSI	CACGAG	1 cut(s)	160
BssT1I	CCWWGG	3 cut(s)	694, 1377, 1745
Bst2BI	CACGAG	1 cut(s)	160
Bst2UI	CCWGG	1 cut(s)	643
Bst4CI	ACNGT	6 cut(s)	382, 575, 815, 860, 1438, 1771
Bst6I	CTCTTC	3 cut(s)	58, 1460, 1478
BstACI	GRCGYC	1 cut(s)	279
BstBAI	YACGTR	1 cut(s)	1627
BstC8I	GCNNGC	7 cut(s)	324, 388, 459, 522, 701, 1009, 1181
BstDEI	CTNAG	6 cut(s)	851, 1099, 1218, 1392, 1575, 1583
BstDSI	CCRYGG	1 cut(s)	1325
BstEII	GGTNACC	1 cut(s)	1055
BstF5I	GGATG	5 cut(s)	624, 1038, 1275, 1279, 1720
BstFNI	CGCG	2 cut(s)	86, 544
BstH2I	RGCGCY	1 cut(s)	282
BstHHI	GCGC	2 cut(s)	281, 1185
BstKTI	GATC	9 cut(s)	152, 414, 808, 955, 1114, 1294, 1509, 1683, 1788
BstMAI	GTCTC	1 cut(s)	406
BstMBI	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
BstMCI	CGRYCG	1 cut(s)	1108
BstMWI	GCNNNNNNNGC	5 cut(s)	267, 276, 454, 838, 1630
BstNI	CCWGG	1 cut(s)	643
BstNSI	RCATGY	3 cut(s)	390, 670, 1153
BstPI	GGTNACC	1 cut(s)	1055
BstSCI	CCNGG	1 cut(s)	641
BstSLI	GKGCMC	3 cut(s)	47, 350, 1626
BstUI	CGCG	2 cut(s)	86, 544
BstV2I	GAAGAC	5 cut(s)	540, 1436, 1649, 1661, 1667
BstX2I	RGATCY	3 cut(s)	952, 1111, 1291
BstXI	CCANNNNNNTGG	3 cut(s)	40, 322, 1646
BstYI	RGATCY	3 cut(s)	952, 1111, 1291
BsuRI	GGCC	9 cut(s)	261, 270, 308, 326, 356, 457, 491, 966, 1453
BtgI	CCRYGG	1 cut(s)	1325
BtrI	CACGTC	1 cut(s)	877
BtsCI	GGATG	5 cut(s)	624, 1038, 1275, 1279, 1720
BtsI	GCAGTG	2 cut(s)	443, 1729
BtsIMutI	CAGTG	6 cut(s)	21, 350, 443, 571, 1443, 1729
Cac8I	GCNNGC	7 cut(s)	324, 388, 459, 522, 701, 1009, 1181
CfoI	GCGC	2 cut(s)	281, 1185
Cfr10I	RCCGGY	4 cut(s)	397, 921, 1103, 1610
Cfr13I	GGNCC	6 cut(s)	325, 430, 847, 1144, 1189, 1470
Csp6I	GTAC	6 cut(s)	484, 670, 811, 919, 1650, 1742
CspAI	ACCGGT	1 cut(s)	397
CspCI	CAANNNNNGTGG	2 cut(s)	271, 306
CviAII	CATG	7 cut(s)	387, 517, 550, 667, 916, 1084, 1150
CviQI	GTAC	6 cut(s)	484, 670, 811, 919, 1650, 1742
DdeI	CTNAG	6 cut(s)	851, 1099, 1218, 1392, 1575, 1583
DinI	GGCGCC	1 cut(s)	280
DpnI	GATC	9 cut(s)	151, 413, 807, 954, 1113, 1293, 1508, 1682, 1787
DpnII	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
DrdI	GACNNNNNNGTC	1 cut(s)	629
DseDI	GACNNNNNNGTC	1 cut(s)	629
EaeI	YGGCCR	3 cut(s)	354, 489, 964
Eam1104I	CTCTTC	3 cut(s)	58, 1460, 1478
EarI	CTCTTC	3 cut(s)	58, 1460, 1478
Eco130I	CCWWGG	3 cut(s)	694, 1377, 1745
Eco147I	AGGCCT	1 cut(s)	457
Eco31I	GGTCTC	1 cut(s)	406
Eco47I	GGWCC	5 cut(s)	430, 847, 1144, 1189, 1470
Eco72I	CACGTG	1 cut(s)	1627
Eco91I	GGTNACC	1 cut(s)	1055
EcoO109I	RGGNCCY	1 cut(s)	1189
EcoO65I	GGTNACC	1 cut(s)	1055
EcoRII	CCWGG	1 cut(s)	641
EcoT14I	CCWWGG	3 cut(s)	694, 1377, 1745
EgeI	GGCGCC	1 cut(s)	280
EheI	GGCGCC	1 cut(s)	280
ErhI	CCWWGG	3 cut(s)	694, 1377, 1745
FaeI	CATG	7 cut(s)	390, 520, 553, 670, 919, 1087, 1153
FaqI	GGGAC	2 cut(s)	758, 1283
FatI	CATG	7 cut(s)	386, 516, 549, 666, 915, 1083, 1149
FauI	CCCGC	2 cut(s)	327, 549
FbaI	TGATCA	1 cut(s)	1506
Fnu4HI	GCNGC	3 cut(s)	268, 462, 1631
FokI	GGATG	5 cut(s)	611, 1025, 1262, 1266, 1727
Fsp4HI	GCNGC	3 cut(s)	268, 462, 1631
FspBI	CTAG	2 cut(s)	908, 1746
GlaI	GCGC	2 cut(s)	280, 1184
GluI	GCNGC	3 cut(s)	268, 462, 1631
HaeII	RGCGCY	1 cut(s)	282
HaeIII	GGCC	9 cut(s)	261, 270, 308, 326, 356, 457, 491, 966, 1453
HapII	CCGG	9 cut(s)	369, 398, 614, 822, 922, 936, 950, 1104, 1611
HhaI	GCGC	2 cut(s)	281, 1185
Hin1I	GRCGYC	1 cut(s)	279
Hin1II	CATG	7 cut(s)	390, 520, 553, 670, 919, 1087, 1153
Hin6I	GCGC	2 cut(s)	279, 1183
HinP1I	GCGC	2 cut(s)	279, 1183
HinfI	GANTC	7 cut(s)	690, 746, 904, 1424, 1577, 1585, 1666
HpaII	CCGG	9 cut(s)	369, 398, 614, 822, 922, 936, 950, 1104, 1611
HphI	GGTGA	5 cut(s)	355, 386, 1067, 1744, 1759
Hpy166II	GTNNAC	7 cut(s)	348, 395, 571, 847, 880, 1144, 1624
Hpy188I	TCNGA	4 cut(s)	60, 1175, 1603, 1671
Hpy8I	GTNNAC	7 cut(s)	348, 395, 571, 847, 880, 1144, 1624
Hpy99I	CGWCG	1 cut(s)	1238
HpyAV	CCTTC	5 cut(s)	124, 319, 430, 667, 1471
HpyCH4III	ACNGT	6 cut(s)	382, 575, 815, 860, 1438, 1771
HpyCH4IV	ACGT	5 cut(s)	876, 1140, 1418, 1626, 1736
HpyCH4V	TGCA	7 cut(s)	11, 165, 348, 585, 1137, 1415, 1624
HpyF10VI	GCNNNNNNNGC	5 cut(s)	267, 276, 454, 838, 1630
HpyF3I	CTNAG	6 cut(s)	851, 1099, 1218, 1392, 1575, 1583
HpySE526I	ACGT	5 cut(s)	876, 1140, 1418, 1626, 1736
Hsp92I	GRCGYC	1 cut(s)	279
Hsp92II	CATG	7 cut(s)	390, 520, 553, 670, 919, 1087, 1153
HspAI	GCGC	2 cut(s)	279, 1183
KasI	GGCGCC	1 cut(s)	278
Kpn2I	TCCGGA	1 cut(s)	613
KpnI	GGTACC	1 cut(s)	1745
Ksp22I	TGATCA	1 cut(s)	1506
Kzo9I	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
LguI	GCTCTTC	1 cut(s)	1478
LweI	GCATC	3 cut(s)	1204, 1284, 1561
MaeI	CTAG	2 cut(s)	908, 1746
MaeII	ACGT	5 cut(s)	876, 1140, 1418, 1626, 1736
MaeIII	GTNAC	4 cut(s)	472, 815, 1055, 1732
MalI	GATC	9 cut(s)	151, 413, 807, 954, 1113, 1293, 1508, 1682, 1787
MboI	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
MflI	RGATCY	3 cut(s)	952, 1111, 1291
MhlI	GDGCHC	3 cut(s)	47, 350, 1626
MlsI	TGGCCA	1 cut(s)	491
MluCI	AATT	6 cut(s)	73, 220, 255, 739, 765, 1604
MluNI	TGGCCA	1 cut(s)	491
Mly113I	GGCGCC	1 cut(s)	279
MlyI	GAGTC	4 cut(s)	740, 1418, 1586, 1594
MmeI	TCCRAC	2 cut(s)	216, 1779
Mox20I	TGGCCA	1 cut(s)	491
MroI	TCCGGA	1 cut(s)	613
MscI	TGGCCA	1 cut(s)	491
MseI	TTAA	2 cut(s)	1563, 1782
MslI	CAYNNNNRTG	1 cut(s)	38
Msp20I	TGGCCA	1 cut(s)	491
MspA1I	CMGCKG	2 cut(s)	322, 1396
MspI	CCGG	9 cut(s)	369, 398, 614, 822, 922, 936, 950, 1104, 1611
MspR9I	CCNGG	1 cut(s)	643
MvaI	CCWGG	1 cut(s)	643
MvnI	CGCG	2 cut(s)	86, 544
MwoI	GCNNNNNNNGC	5 cut(s)	267, 276, 454, 838, 1630
NarI	GGCGCC	1 cut(s)	279
NdeII	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
NlaIII	CATG	7 cut(s)	390, 520, 553, 670, 919, 1087, 1153
NlaIV	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
NmeAIII	GCCGAG	4 cut(s)	243, 1270, 1383, 1485
NmuCI	GTSAC	4 cut(s)	472, 815, 1055, 1732
NspI	RCATGY	3 cut(s)	390, 670, 1153
PaeI	GCATGC	1 cut(s)	390
PceI	AGGCCT	1 cut(s)	457
PciSI	GCTCTTC	1 cut(s)	1478
PcsI	WCGNNNNNNNCGW	3 cut(s)	864, 1242, 1424
PfeI	GAWTC	3 cut(s)	690, 904, 1666
PinAI	ACCGGT	1 cut(s)	397
PkrI	GCNGC	3 cut(s)	269, 463, 1632
PleI	GAGTC	4 cut(s)	740, 1418, 1585, 1593
PluTI	GGCGCC	1 cut(s)	282
PmaCI	CACGTG	1 cut(s)	1627
PmlI	CACGTG	1 cut(s)	1627
PpsI	GAGTC	4 cut(s)	740, 1418, 1585, 1593
Ppu21I	YACGTR	1 cut(s)	1627
PpuMI	RGGWCCY	1 cut(s)	1189
PshBI	ATTAAT	1 cut(s)	1563
Psp1406I	AACGTT	1 cut(s)	1418
Psp5II	RGGWCCY	1 cut(s)	1189
Psp6I	CCWGG	1 cut(s)	641
PspCI	CACGTG	1 cut(s)	1627
PspEI	GGTNACC	1 cut(s)	1055
PspGI	CCWGG	1 cut(s)	641
PspN4I	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
PspPI	GGNCC	6 cut(s)	325, 430, 847, 1144, 1189, 1470
PspPPI	RGGWCCY	1 cut(s)	1189
PsuI	RGATCY	3 cut(s)	952, 1111, 1291
PvuII	CAGCTG	1 cut(s)	1396
RsaI	GTAC	6 cut(s)	485, 671, 812, 920, 1651, 1743
RsaNI	GTAC	6 cut(s)	484, 670, 811, 919, 1650, 1742
RseI	CAYNNNNRTG	1 cut(s)	38
SapI	GCTCTTC	1 cut(s)	1478
SaqAI	TTAA	2 cut(s)	1563, 1782
SatI	GCNGC	3 cut(s)	268, 462, 1631
Sau3AI	GATC	9 cut(s)	149, 411, 805, 952, 1111, 1291, 1506, 1680, 1785
Sau96I	GGNCC	6 cut(s)	325, 430, 847, 1144, 1189, 1470
ScaI	AGTACT	1 cut(s)	485
SchI	GAGTC	4 cut(s)	740, 1418, 1586, 1594
ScrFI	CCNGG	1 cut(s)	643
SduI	GDGCHC	3 cut(s)	47, 350, 1626
SfaNI	GCATC	3 cut(s)	1204, 1284, 1561
SfiI	GGCCNNNNNGGCC	1 cut(s)	267
SfoI	GGCGCC	1 cut(s)	280
SinI	GGWCC	5 cut(s)	430, 847, 1144, 1189, 1470
SmiMI	CAYNNNNRTG	1 cut(s)	38
SmlI	CTYRAG	1 cut(s)	507
SmoI	CTYRAG	1 cut(s)	507
SphI	GCATGC	1 cut(s)	390
Sse9I	AATT	6 cut(s)	73, 220, 255, 739, 765, 1604
SseBI	AGGCCT	1 cut(s)	457
SspDI	GGCGCC	1 cut(s)	278
SspMI	CTAG	2 cut(s)	908, 1746
StuI	AGGCCT	1 cut(s)	457
StyD4I	CCNGG	1 cut(s)	641
StyI	CCWWGG	3 cut(s)	694, 1377, 1745
TaaI	ACNGT	6 cut(s)	382, 575, 815, 860, 1438, 1771
TaiI	ACGT	5 cut(s)	879, 1143, 1421, 1629, 1739
TaqI	TCGA	6 cut(s)	1212, 1337, 1427, 1551, 1588, 1788
TasI	AATT	6 cut(s)	73, 220, 255, 739, 765, 1604
TatI	WGTACW	2 cut(s)	483, 669
TauI	GCSGC	3 cut(s)	270, 464, 1633
TfiI	GAWTC	3 cut(s)	690, 904, 1666
Tru1I	TTAA	2 cut(s)	1563, 1782
Tru9I	TTAA	2 cut(s)	1563, 1782
TscAI	CASTG	6 cut(s)	28, 357, 450, 578, 1443, 1729
TseFI	GTSAC	4 cut(s)	472, 815, 1055, 1732
Tsp45I	GTSAC	4 cut(s)	472, 815, 1055, 1732
TspGWI	ACGGA	3 cut(s)	491, 1314, 1700
TspRI	CASTG	6 cut(s)	28, 357, 450, 578, 1443, 1729
VneI	GTGCAC	2 cut(s)	346, 1622
VpaK11BI	GGWCC	5 cut(s)	430, 847, 1144, 1189, 1470
VspI	ATTAAT	1 cut(s)	1563
XapI	RAATTY	1 cut(s)	1604
XceI	RCATGY	3 cut(s)	390, 670, 1153
XcmI	CCANNNNNNNNNTGG	2 cut(s)	349, 738
XmaJI	CCTAGG	1 cut(s)	1745
XspI	CTAG	2 cut(s)	908, 1746
ZrmI	AGTACT	1 cut(s)	485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.