Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Reverse (-)

38631151 .. 38632866

Gene Structure 1716 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1299100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGAAAACTACTTAAGCCTTCCTAATTCAAATCAGTCTCGGAAGCCTCTACGGTGGAGTGGCTGGTCTTGGCAGCGACCCCTTCGCTGTTGCAAAGCCGGAGTGGCTGGTCTTGGCAGCGACCCCTTCGCTGTTGCAAAGCCAGTGTTGCCGCCTGACCTAGCCAAATGCGGAGCTGCGGACTTTCCAAGTGGAGCAGTCCCGATCAACTGTTGCCCGCCAACGTCCCAAAAAATCGTAGACTTCAAATTCCCCTCCCCTACCAAACTCCGCGTCAGGCCGGCAGCTCACACCGTGGATAAAGCCTACATCGAAAAATATTCAAAAGCCATCGAGCTCATGAAAGCCCTCCCGGACGACGATCCGCGTAGCTTCACCCAGCAAGCCGATCTCCACTGTGCCTATTGCGACGGCGCGTACGACCAAGTCGGCTTCCCCAACCTCGAGCTCCAAATCCATCAATGCTGGCTTTTCTTCCCCTTCCATCGTTACTACGTATACTTCTACGAAAGAATCTTGGCCAAACTCATAGACGATCCGACGTTCGCGTTGCCGTTTTGGAACTGGGACGCGCCAGCTGGCATGCAACTCCCTGCCTTGTTCGCTAACCCGGACTCTCCGCTTTACGACGAGCTCCGCGCTGCCAGCCATCAGCCGCCGACTCTCATCGATCTTGACTTCAACGGCACGGATGAAACAATGTCCAAGGATGCTCAAATCGACGCCAACCTCAAAATCATGTATAGGCAGATGGTTTCCAACTCCAAGAAACCGCTGTTGTTCTTTGGTTCGCCCTACAGGGCTGACACTGAACCAGATCCAGGGGGCGGTTCAATCGAAACGACCCCACATGGTCCGGTTCATTTATGGACCGGAGATAACACGCAGCCTAATTTTGAAGACATGGGGAATTTTTACTCCGCTGCAAGGGATCCAATATTTTTTTCGCACCATTCGAATATAGATCGAATGTGGAATATTTGGAAAAGTATAGGGACTAAAAATAACGATATTAACGATAAGGATTGGTTGGATACGGGGTTTTTGTTTTATGACGAGAATGCTGAGCTTGTTAGGGTCACGGTGAGGGACACTCTTGATAATAAAAAGCTAGGGTATACGTATGAAGATGTTGAGATTCCATGGCTCAAGTCTAGACCGACGCCACGTCGGACAAAGCTTGCGAGAAAGGCAAAGGCGGCTGGAGTGGCGAAGGCCACTGAGACGACGTCATCAGGGAAGGTGGTGGCGGGTAAAGATTTTCCAATAAATTTGGAGACGAAGATAAGTACGGTGGTGTCAAGGCCGAAGCCGAAGAAGAGGAGCAAGAAGGAGAAAGAGGATGAGGAGGAGATATTGGTGATTCAGGGGATTGAGCTTGACAAAGATGTGGCTGTGAAGTTTGATGTGTATGTGAATGACGTGGACGATGAGGATGCGGCACCGAGTGGACCCGACAAGAGCGAGTTTGCTGGGAGTTTTGTGAGTGTGCCACATAAGCAGAAGGAAAAGAGCAAGAGTTGTTTAAGGTTGGGGTTAACGGACCTGTTGGAGGATTTGGGTGCTGAAGATGATGAGAGTGTGGTGGTGACTTTGGTGCCCAGGTACGGCGCTCAGGCTGTTAAGATCGGTAGCATCAAAATTGAATTTCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

553

Amino Acids

61.9

Weight (kDa)

5.62

Isoelectric Point (pI)

42.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	123 - 329	1.4e-36	Common central domain of tyrosinase
PPO1_DWL	PF12142	337 - 388	6.6e-26	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	421 - 550	3.6e-51	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1232
AccB1I	GGYRCC	2 cut(s)	1441, 1597
AccI	GTMKAC	3 cut(s)	240, 498, 1118
AccII	CGCG	6 cut(s)	273, 367, 416, 548, 572, 639
AclWI	GGATC	5 cut(s)	356, 530, 812, 926, 939
AcoI	YGGCCR	1 cut(s)	519
AcsI	RAATTY	4 cut(s)	248, 910, 1270, 1646
AcuI	CTGAAG	1 cut(s)	1587
AcyI	GRCGYC	3 cut(s)	723, 1163, 1229
AdeI	CACNNNGTG	2 cut(s)	295, 1448
AfaI	GTAC	3 cut(s)	419, 1291, 1607
AfiI	CCNNNNNNNGG	5 cut(s)	821, 827, 927, 1552, 1607
AflII	CTTAAG	1 cut(s)	13
AgsI	TTSAA	7 cut(s)	30, 247, 324, 682, 834, 899, 1646
AhdI	GACNNNNNGTC	1 cut(s)	1167
AjiI	CACGTC	2 cut(s)	1169, 1423
AjnI	CCWGG	2 cut(s)	820, 1601
AjuI	GAANNNNNNNTTGG	2 cut(s)	928, 960
Alw21I	GWGCWC	3 cut(s)	339, 450, 636
Alw26I	GTCTC	3 cut(s)	42, 1217, 1271
AlwI	GGATC	5 cut(s)	356, 530, 812, 926, 939
Ama87I	CYCGRG	1 cut(s)	443
AoxI	GGCC	4 cut(s)	278, 519, 1215, 1304
ApeKI	GCWGC	7 cut(s)	73, 117, 176, 284, 641, 886, 923
ApoI	RAATTY	4 cut(s)	248, 910, 1270, 1646
ArsI	GACNNNNNNTTYG	2 cut(s)	258, 290
AspLEI	GCGC	4 cut(s)	416, 574, 641, 1613
AspS9I	GGNCC	4 cut(s)	854, 870, 1451, 1543
AsuC2I	CCSGG	2 cut(s)	353, 611
AsuHPI	GGTGA	4 cut(s)	367, 1096, 1372, 1600
AsuII	TTCGAA	1 cut(s)	956
AvaI	CYCGRG	1 cut(s)	443
AvaII	GGWCC	4 cut(s)	854, 870, 1451, 1543
BaeGI	GKGCMC	1 cut(s)	1602
BalI	TGGCCA	1 cut(s)	521
BamHI	GGATCC	1 cut(s)	931
BanI	GGYRCC	2 cut(s)	1441, 1597
BanII	GRGCYC	3 cut(s)	339, 450, 636
BbsI	GAAGAC	1 cut(s)	906
Bbv12I	GWGCWC	3 cut(s)	339, 450, 636
BbvI	GCAGC	7 cut(s)	85, 129, 163, 296, 628, 898, 910
BccI	CCATC	5 cut(s)	338, 465, 492, 657, 745
BceAI	ACGGC	4 cut(s)	427, 538, 700, 1624
BcgI	CGANNNNNNTGC	2 cut(s)	1418, 1452
BciT130I	CCWGG	2 cut(s)	822, 1603
BciVI	GTATCC	1 cut(s)	1027
BcnI	CCSGG	2 cut(s)	353, 611
BcoDI	GTCTC	3 cut(s)	42, 1217, 1271
BfaI	CTAG	3 cut(s)	161, 1112, 1155
BfmI	CTRYAG	1 cut(s)	796
BfoI	RGCGCY	1 cut(s)	1614
BfrI	CTTAAG	1 cut(s)	13
BfuI	GTATCC	1 cut(s)	1027
BglI	GCCNNNNNGGC	1 cut(s)	104
BlpI	GCTNAGC	1 cut(s)	1065
Bme1390I	CCNGG	4 cut(s)	353, 611, 822, 1603
Bme18I	GGWCC	4 cut(s)	854, 870, 1451, 1543
BmeRI	GACNNNNNGTC	1 cut(s)	1167
BmeT110I	CYCGRG	1 cut(s)	443
BmgBI	CACGTC	2 cut(s)	1169, 1423
BmgT120I	GGNCC	4 cut(s)	854, 870, 1451, 1543
BmiI	GGNNCC	4 cut(s)	933, 1443, 1453, 1599
BmrFI	CCNGG	4 cut(s)	353, 611, 822, 1603
BmrI	ACTGGG	1 cut(s)	574
BmsI	GCATC	3 cut(s)	700, 1426, 1644
BmuI	ACTGGG	1 cut(s)	574
BpiI	GAAGAC	1 cut(s)	906
BplI	GAGNNNNNCTC	2 cut(s)	1078, 1110
BpmI	CTGGAG	1 cut(s)	1224
Bpu10I	CCTNAGC	1 cut(s)	1614
Bpu1102I	GCTNAGC	1 cut(s)	1065
Bpu14I	TTCGAA	1 cut(s)	956
BpuEI	CTTGAG	1 cut(s)	1133
BpuMI	CCSGG	2 cut(s)	353, 611
Bsa29I	ATCGAT	1 cut(s)	669
BsaAI	YACGTR	2 cut(s)	496, 1122
BsaHI	GRCGYC	3 cut(s)	723, 1163, 1229
BsaJI	CCNNGG	5 cut(s)	294, 705, 821, 1142, 1601
BsaWI	WCCGGW	2 cut(s)	856, 872
Bsc4I	CCNNNNNNNGG	5 cut(s)	821, 827, 927, 1552, 1607
Bse118I	RCCGGY	1 cut(s)	280
Bse1I	ACTGG	2 cut(s)	143, 569
BseBI	CCWGG	2 cut(s)	822, 1603
BseCI	ATCGAT	1 cut(s)	669
BseDI	CCNNGG	5 cut(s)	294, 705, 821, 1142, 1601
BseGI	GGATG	4 cut(s)	697, 715, 1348, 1441
BseLI	CCNNNNNNNGG	5 cut(s)	821, 827, 927, 1552, 1607
BseMII	CTCAG	3 cut(s)	1056, 1212, 1628
BseNI	ACTGG	2 cut(s)	143, 569
BseRI	GAGGAG	3 cut(s)	1336, 1361, 1364
BseSI	GKGCMC	1 cut(s)	1602
BseXI	GCAGC	7 cut(s)	85, 129, 163, 296, 628, 898, 910
BseYI	CCCAGC	2 cut(s)	378, 1472
Bsh1236I	CGCG	6 cut(s)	273, 367, 416, 548, 572, 639
BshFI	GGCC	4 cut(s)	280, 521, 1217, 1306
BshNI	GGYRCC	2 cut(s)	1441, 1597
BshVI	ATCGAT	1 cut(s)	669
BsiHKAI	GWGCWC	3 cut(s)	339, 450, 636
BsiHKCI	CYCGRG	1 cut(s)	443
BsiSI	CCGG	6 cut(s)	99, 281, 353, 611, 857, 873
BsiWI	CGTACG	1 cut(s)	417
BslFI	GGGAC	5 cut(s)	185, 211, 581, 1009, 1103
BslI	CCNNNNNNNGG	5 cut(s)	821, 827, 927, 1552, 1607
BsmAI	GTCTC	3 cut(s)	42, 1217, 1271
BsmBI	CGTCTC	2 cut(s)	1217, 1271
BsmFI	GGGAC	5 cut(s)	185, 211, 581, 1009, 1103
BsmI	GAATGC	1 cut(s)	1066
BsnI	GGCC	4 cut(s)	280, 521, 1217, 1306
BsoBI	CYCGRG	1 cut(s)	443
Bsp119I	TTCGAA	1 cut(s)	956
Bsp1286I	GDGCHC	4 cut(s)	339, 450, 636, 1602
Bsp143I	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
Bsp1720I	GCTNAGC	1 cut(s)	1065
Bsp19I	CCATGG	1 cut(s)	1142
BspANI	GGCC	4 cut(s)	280, 521, 1217, 1306
BspCNI	CTCAG	3 cut(s)	1057, 1213, 1627
BspDI	ATCGAT	1 cut(s)	669
BspFNI	CGCG	6 cut(s)	273, 367, 416, 548, 572, 639
BspHI	TCATGA	1 cut(s)	339
BspLI	GGNNCC	4 cut(s)	933, 1443, 1453, 1599
BspPI	GGATC	5 cut(s)	356, 530, 812, 926, 939
BspT104I	TTCGAA	1 cut(s)	956
BspT107I	GGYRCC	2 cut(s)	1441, 1597
BspTI	CTTAAG	1 cut(s)	13
BsrFI	RCCGGY	1 cut(s)	280
BsrI	ACTGG	2 cut(s)	143, 569
BssAI	RCCGGY	1 cut(s)	280
BssECI	CCNNGG	5 cut(s)	294, 705, 821, 1142, 1601
BssMI	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
BssNAI	GTATAC	2 cut(s)	499, 1119
BssNI	GRCGYC	3 cut(s)	723, 1163, 1229
BssT1I	CCWWGG	2 cut(s)	705, 1142
Bst1107I	GTATAC	2 cut(s)	499, 1119
Bst2UI	CCWGG	2 cut(s)	822, 1603
Bst4CI	ACNGT	6 cut(s)	54, 212, 295, 398, 1084, 1294
Bst6I	CTCTTC	1 cut(s)	1313
BstACI	GRCGYC	3 cut(s)	723, 1163, 1229
BstAFI	CTTAAG	1 cut(s)	13
BstBAI	YACGTR	2 cut(s)	496, 1122
BstBI	TTCGAA	1 cut(s)	956
BstC8I	GCNNGC	9 cut(s)	218, 282, 384, 467, 576, 580, 584, 646, 1182
BstDEI	CTNAG	3 cut(s)	1065, 1221, 1614
BstDSI	CCRYGG	2 cut(s)	294, 1142
BstENI	CCTNNNNNAGG	1 cut(s)	1550
BstF5I	GGATG	4 cut(s)	697, 715, 1348, 1441
BstFNI	CGCG	6 cut(s)	273, 367, 416, 548, 572, 639
BstH2I	RGCGCY	1 cut(s)	1614
BstHHI	GCGC	4 cut(s)	416, 574, 641, 1613
BstKTI	GATC	9 cut(s)	207, 364, 391, 538, 673, 820, 934, 967, 1629
BstMAI	GTCTC	3 cut(s)	42, 1217, 1271
BstMBI	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
BstMWI	GCNNNNNNNGC	8 cut(s)	104, 148, 602, 645, 1190, 1199, 1208, 1498
BstNI	CCWGG	2 cut(s)	822, 1603
BstNSI	RCATGY	1 cut(s)	586
BstSCI	CCNGG	4 cut(s)	351, 609, 820, 1601
BstSFI	CTRYAG	1 cut(s)	796
BstSLI	GKGCMC	1 cut(s)	1602
BstSNI	TACGTA	2 cut(s)	496, 1122
BstUI	CGCG	6 cut(s)	273, 367, 416, 548, 572, 639
BstV1I	GCAGC	7 cut(s)	85, 129, 163, 296, 628, 898, 910
BstV2I	GAAGAC	1 cut(s)	906
BstX2I	RGATCY	2 cut(s)	817, 931
BstYI	RGATCY	2 cut(s)	817, 931
BstZ17I	GTATAC	2 cut(s)	499, 1119
Bsu15I	ATCGAT	1 cut(s)	669
BsuI	GTATCC	1 cut(s)	1027
BsuRI	GGCC	4 cut(s)	280, 521, 1217, 1306
BsuTUI	ATCGAT	1 cut(s)	669
BtgI	CCRYGG	2 cut(s)	294, 1142
BtrI	CACGTC	2 cut(s)	1169, 1423
BtsCI	GGATG	4 cut(s)	697, 715, 1348, 1441
BtsIMutI	CAGTG	4 cut(s)	150, 394, 807, 1218
Cac8I	GCNNGC	9 cut(s)	218, 282, 384, 467, 576, 580, 584, 646, 1182
CciI	TCATGA	1 cut(s)	339
CfoI	GCGC	4 cut(s)	416, 574, 641, 1613
Cfr10I	RCCGGY	1 cut(s)	280
Cfr13I	GGNCC	4 cut(s)	854, 870, 1451, 1543
ClaI	ATCGAT	1 cut(s)	669
CseI	GACGC	4 cut(s)	262, 578, 731, 1171
Csp6I	GTAC	3 cut(s)	418, 1290, 1606
CviAII	CATG	6 cut(s)	340, 583, 739, 851, 904, 1143
CviQI	GTAC	3 cut(s)	418, 1290, 1606
DdeI	CTNAG	3 cut(s)	1065, 1221, 1614
DpnI	GATC	9 cut(s)	206, 363, 390, 537, 672, 819, 933, 966, 1628
DpnII	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
DraIII	CACNNNGTG	2 cut(s)	295, 1448
DriI	GACNNNNNGTC	1 cut(s)	1167
EaeI	YGGCCR	1 cut(s)	519
Eam1104I	CTCTTC	1 cut(s)	1313
Eam1105I	GACNNNNNGTC	1 cut(s)	1167
EarI	CTCTTC	1 cut(s)	1313
Ecl136II	GAGCTC	3 cut(s)	337, 448, 634
Eco105I	TACGTA	2 cut(s)	496, 1122
Eco130I	CCWWGG	2 cut(s)	705, 1142
Eco24I	GRGCYC	3 cut(s)	339, 450, 636
Eco47I	GGWCC	4 cut(s)	854, 870, 1451, 1543
Eco53kI	GAGCTC	3 cut(s)	337, 448, 634
Eco57I	CTGAAG	1 cut(s)	1587
Eco88I	CYCGRG	1 cut(s)	443
EcoICRI	GAGCTC	3 cut(s)	337, 448, 634
EcoNI	CCTNNNNNAGG	1 cut(s)	1550
EcoRII	CCWGG	2 cut(s)	820, 1601
EcoT14I	CCWWGG	2 cut(s)	705, 1142
EcoT38I	GRGCYC	3 cut(s)	339, 450, 636
ErhI	CCWWGG	2 cut(s)	705, 1142
Esp3I	CGTCTC	2 cut(s)	1217, 1271
FaeI	CATG	6 cut(s)	343, 586, 742, 854, 907, 1146
FaqI	GGGAC	5 cut(s)	185, 211, 581, 1009, 1103
FatI	CATG	6 cut(s)	339, 582, 738, 850, 903, 1142
FauI	CCCGC	2 cut(s)	225, 1243
FblI	GTMKAC	3 cut(s)	240, 498, 1118
FokI	GGATG	4 cut(s)	704, 722, 1355, 1448
FriOI	GRGCYC	3 cut(s)	339, 450, 636
FspBI	CTAG	3 cut(s)	161, 1112, 1155
GlaI	GCGC	4 cut(s)	415, 573, 640, 1612
GsaI	CCCAGC	2 cut(s)	382, 1476
GsuI	CTGGAG	1 cut(s)	1224
HaeII	RGCGCY	1 cut(s)	1614
HaeIII	GGCC	4 cut(s)	280, 521, 1217, 1306
HapII	CCGG	6 cut(s)	99, 281, 353, 611, 857, 873
HgaI	GACGC	4 cut(s)	262, 578, 731, 1171
HhaI	GCGC	4 cut(s)	416, 574, 641, 1613
Hin1I	GRCGYC	3 cut(s)	723, 1163, 1229
Hin1II	CATG	6 cut(s)	343, 586, 742, 854, 907, 1146
Hin6I	GCGC	4 cut(s)	414, 572, 639, 1611
HinP1I	GCGC	4 cut(s)	414, 572, 639, 1611
HincII	GTYRAC	1 cut(s)	1539
HindII	GTYRAC	1 cut(s)	1539
HindIII	AAGCTT	1 cut(s)	1178
HinfI	GANTC	5 cut(s)	513, 614, 661, 1138, 1363
HpaI	GTTAAC	1 cut(s)	1539
HpaII	CCGG	6 cut(s)	99, 281, 353, 611, 857, 873
HphI	GGTGA	4 cut(s)	367, 1096, 1372, 1600
Hpy166II	GTNNAC	6 cut(s)	241, 499, 1119, 1426, 1451, 1539
Hpy188I	TCNGA	3 cut(s)	42, 540, 1173
Hpy188III	TCNNGA	5 cut(s)	202, 340, 674, 1097, 1155
Hpy8I	GTNNAC	6 cut(s)	241, 499, 1119, 1426, 1451, 1539
Hpy99I	CGWCG	8 cut(s)	362, 413, 544, 632, 725, 1165, 1173, 1231
HpyAV	CCTTC	8 cut(s)	29, 92, 136, 490, 1207, 1234, 1324, 1498
HpyCH4III	ACNGT	6 cut(s)	54, 212, 295, 398, 1084, 1294
HpyCH4IV	ACGT	7 cut(s)	224, 495, 542, 1121, 1168, 1229, 1422
HpyCH4V	TGCA	4 cut(s)	93, 137, 586, 926
HpyF10VI	GCNNNNNNNGC	8 cut(s)	104, 148, 602, 645, 1190, 1199, 1208, 1498
HpyF3I	CTNAG	3 cut(s)	1065, 1221, 1614
HpySE526I	ACGT	7 cut(s)	224, 495, 542, 1121, 1168, 1229, 1422
Hsp92I	GRCGYC	3 cut(s)	723, 1163, 1229
Hsp92II	CATG	6 cut(s)	343, 586, 742, 854, 907, 1146
HspAI	GCGC	4 cut(s)	414, 572, 639, 1611
KroI	GCCGGC	1 cut(s)	280
KroNI	GCCGGC	1 cut(s)	282
KspAI	GTTAAC	1 cut(s)	1539
Kzo9I	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
LmnI	GCTCC	5 cut(s)	173, 194, 453, 639, 1323
Lsp1109I	GCAGC	7 cut(s)	85, 129, 163, 296, 628, 898, 910
LweI	GCATC	3 cut(s)	700, 1426, 1644
MaeI	CTAG	3 cut(s)	161, 1112, 1155
MaeII	ACGT	7 cut(s)	224, 495, 542, 1121, 1168, 1229, 1422
MaeIII	GTNAC	3 cut(s)	488, 1078, 1588
MalI	GATC	9 cut(s)	206, 363, 390, 537, 672, 819, 933, 966, 1628
MboI	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
MboII	GAAGA	7 cut(s)	466, 911, 1139, 1294, 1327, 1330, 1580
MflI	RGATCY	2 cut(s)	817, 931
MhlI	GDGCHC	4 cut(s)	339, 450, 636, 1602
MlsI	TGGCCA	1 cut(s)	521
MluCI	AATT	7 cut(s)	25, 248, 892, 910, 1270, 1641, 1646
MluNI	TGGCCA	1 cut(s)	521
MlyI	GAGTC	2 cut(s)	608, 655
MmeI	TCCRAC	5 cut(s)	563, 783, 1011, 1151, 1530
Mox20I	TGGCCA	1 cut(s)	521
MroNI	GCCGGC	1 cut(s)	280
MscI	TGGCCA	1 cut(s)	521
MseI	TTAA	5 cut(s)	14, 1014, 1526, 1538, 1623
Msp20I	TGGCCA	1 cut(s)	521
MspA1I	CMGCKG	3 cut(s)	578, 775, 923
MspCI	CTTAAG	1 cut(s)	13
MspI	CCGG	6 cut(s)	99, 281, 353, 611, 857, 873
MspR9I	CCNGG	4 cut(s)	353, 611, 822, 1603
Mva1269I	GAATGC	1 cut(s)	1066
MvaI	CCWGG	2 cut(s)	822, 1603
MvnI	CGCG	6 cut(s)	273, 367, 416, 548, 572, 639
MwoI	GCNNNNNNNGC	8 cut(s)	104, 148, 602, 645, 1190, 1199, 1208, 1498
NaeI	GCCGGC	1 cut(s)	282
NciI	CCSGG	2 cut(s)	353, 611
NcoI	CCATGG	1 cut(s)	1142
NdeII	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
NgoMIV	GCCGGC	1 cut(s)	280
NlaIII	CATG	6 cut(s)	343, 586, 742, 854, 907, 1146
NlaIV	GGNNCC	4 cut(s)	933, 1443, 1453, 1599
NmuCI	GTSAC	2 cut(s)	1078, 1588
NspI	RCATGY	1 cut(s)	586
NspV	TTCGAA	1 cut(s)	956
PaeI	GCATGC	1 cut(s)	586
PaeR7I	CTCGAG	1 cut(s)	443
PagI	TCATGA	1 cut(s)	339
PcsI	WCGNNNNNNNCGW	3 cut(s)	417, 953, 1014
PctI	GAATGC	1 cut(s)	1066
PdiI	GCCGGC	1 cut(s)	282
PfeI	GAWTC	3 cut(s)	513, 1138, 1363
Pfl23II	CGTACG	1 cut(s)	417
PflFI	GACNNNGTC	2 cut(s)	425, 1228
PfoI	TCCNGGA	1 cut(s)	351
PleI	GAGTC	2 cut(s)	608, 655
PpsI	GAGTC	2 cut(s)	608, 655
Ppu21I	YACGTR	2 cut(s)	496, 1122
Psp124BI	GAGCTC	3 cut(s)	339, 450, 636
Psp6I	CCWGG	2 cut(s)	820, 1601
PspFI	CCCAGC	2 cut(s)	378, 1472
PspGI	CCWGG	2 cut(s)	820, 1601
PspLI	CGTACG	1 cut(s)	417
PspN4I	GGNNCC	4 cut(s)	933, 1443, 1453, 1599
PspPI	GGNCC	4 cut(s)	854, 870, 1451, 1543
PspXI	VCTCGAGB	1 cut(s)	443
PsuI	RGATCY	2 cut(s)	817, 931
PsyI	GACNNNGTC	2 cut(s)	425, 1228
PvuII	CAGCTG	1 cut(s)	578
RsaI	GTAC	3 cut(s)	419, 1291, 1607
RsaNI	GTAC	3 cut(s)	418, 1290, 1606
SacI	GAGCTC	3 cut(s)	339, 450, 636
SaqAI	TTAA	5 cut(s)	14, 1014, 1526, 1538, 1623
Sau3AI	GATC	9 cut(s)	204, 361, 388, 535, 670, 817, 931, 964, 1626
Sau96I	GGNCC	4 cut(s)	854, 870, 1451, 1543
SchI	GAGTC	2 cut(s)	608, 655
ScrFI	CCNGG	4 cut(s)	353, 611, 822, 1603
SduI	GDGCHC	4 cut(s)	339, 450, 636, 1602
SfaNI	GCATC	3 cut(s)	700, 1426, 1644
SfcI	CTRYAG	1 cut(s)	796
Sfr274I	CTCGAG	1 cut(s)	443
SfuI	TTCGAA	1 cut(s)	956
SinI	GGWCC	4 cut(s)	854, 870, 1451, 1543
SlaI	CTCGAG	1 cut(s)	443
SmlI	CTYRAG	3 cut(s)	13, 443, 1148
SmoI	CTYRAG	3 cut(s)	13, 443, 1148
SnaBI	TACGTA	2 cut(s)	496, 1122
SphI	GCATGC	1 cut(s)	586
Sse9I	AATT	7 cut(s)	25, 248, 892, 910, 1270, 1641, 1646
SspI	AATATT	3 cut(s)	320, 939, 979
SspMI	CTAG	3 cut(s)	161, 1112, 1155
SstI	GAGCTC	3 cut(s)	339, 450, 636
StyD4I	CCNGG	4 cut(s)	351, 609, 820, 1601
StyI	CCWWGG	2 cut(s)	705, 1142
TaaI	ACNGT	6 cut(s)	54, 212, 295, 398, 1084, 1294
TaiI	ACGT	7 cut(s)	227, 498, 545, 1124, 1171, 1232, 1425
TaqI	TCGA	8 cut(s)	312, 333, 444, 669, 720, 837, 956, 967
TaqII	GACCGA	1 cut(s)	1174
TasI	AATT	7 cut(s)	25, 248, 892, 910, 1270, 1641, 1646
TauI	GCSGC	4 cut(s)	154, 658, 1202, 1442
TfiI	GAWTC	3 cut(s)	513, 1138, 1363
Tru1I	TTAA	5 cut(s)	14, 1014, 1526, 1538, 1623
Tru9I	TTAA	5 cut(s)	14, 1014, 1526, 1538, 1623
TscAI	CASTG	4 cut(s)	150, 401, 814, 1225
TseFI	GTSAC	2 cut(s)	1078, 1588
TseI	GCWGC	7 cut(s)	73, 117, 176, 284, 641, 886, 923
Tsp45I	GTSAC	2 cut(s)	1078, 1588
TspDTI	ATGAA	4 cut(s)	356, 708, 851, 1140
TspGWI	ACGGA	2 cut(s)	704, 1556
TspRI	CASTG	4 cut(s)	150, 401, 814, 1225
Tth111I	GACNNNGTC	2 cut(s)	425, 1228
Vha464I	CTTAAG	1 cut(s)	13
VpaK11BI	GGWCC	4 cut(s)	854, 870, 1451, 1543
XagI	CCTNNNNNAGG	1 cut(s)	1550
XapI	RAATTY	4 cut(s)	248, 910, 1270, 1646
XbaI	TCTAGA	1 cut(s)	1154
XceI	RCATGY	1 cut(s)	586
XhoI	CTCGAG	1 cut(s)	443
XmiI	GTMKAC	3 cut(s)	240, 498, 1118
XspI	CTAG	3 cut(s)	161, 1112, 1155
ZraI	GACGTC	1 cut(s)	1230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.