Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000836

Physical Location & Seq

Genomic Coordinates Forward (+)

44703232 .. 44705756

Gene Structure 2525 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1319300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGCTTCTGCATCCTCTCCCGCTACTGCTACCACCACCACCATGGGCACTTACTCTTCTTTGATCATCTCCACCAATTCCTTCTCTGCCTTCCTCCCAAACAAATCCCAACTTTCCTTCTCTGGAAAAAGCAAGCACTACATTGCACGTAGATCATCAATCTCTTGCAAAGCCACAAACCCTAATTATAATAATGAGCAAGATCAACAACAATCTTCTAATTTGTTGGGAAAATTGGACAGGAGAAATGTCCTAATTGGCCTGGGCGGCCTCTACGGGGCCACCACCCTCGCCCCAAAGCCGTTGGCCTTTGCCAACCCGCTGGCTCCACCCGACCTAACCAAATGTAAGCCGGCCGAAATCACCACCGGAGGTGAAACTGTGGAATGCTGTCCACCGGTCTCCACAAAGATCAAAACCTTCACTCCGGATCGGTCTATCCCACTGCGGACGAGGCCTGCCGCCCATTTGGTCACGGACGAGTACTTGGCCAAGTTCAAGAAAGCTCAAGCCCTCATGCGTGCCTTACCCGAAGACGACCCGCGTAGCATGGTTCAACAAGCTAAAGTTCACTGTGCCTACTGCAATGGTGCTTATCCACAAGTAGGGTTTCCGGACATTGACATCCAAATCCACTTCTCCTGGCTTTTCTTTCCTTTCCACCGCATGTACTTGTATTTCTACGAGAGAATCCTTGGCAAGCTCATTGATGACCCAACTTTCGCTCTCCCATACTGGAATTGGGACTCTCCTGACGGTTTTCCAATTCCCGACATTTACACAGATACAACTTCCCCACTCTATGATCAGTACCGAAACGCCGACCACCAGCCCCCCGTGCTGGTGGATCTCAGCTACGGTGGAACCGATGATGACGTGGACGACCAGACAAGAATAGATGAGAATCTAGCCATCATGTACCGGCAAATGGTTTCCGGTGCCAAAACTCCCCATCTATTTTTCGGCCATGAGTACCGGGCAGGAGACACAACAACAGGGACTTACGCCGGCACCATTGAGAACAGTCCTCATAATAACATCCATCTCTGGTGCGGTGACCCGAACCAGACCCACCAAGAAGACATGGGTAACTTCTACTCCGCCGGTCGGGATCCCCTGTTTTACGCCCACCATTGCAACGTGGACCGCATGTGGAACGTTTGGAAAACCCTCGGAGGCAAGCGCAAGGACCCCACCGACACCGATTGGCTTGACGCTGAGTTTCTGTTCTACGATGAAAACGCCGAGCTTGTGAGCTGTAAAGTTCGGGACAGCCTCAAACCTGAGAAAGATCTTCGTTATACTTACGAGCCTGTTAGTGTTCCGTGGCTGTTCACCAAGCCAACCGCTCGTAAGCCAAAGAGCAAGACAAAAGCCAAGGTGGGGGCTACCCAGCTGACGACAAAGTTCCCGGCCACGTTTGATTCGAAGACGACGGTGGAGGTGGCGAGGCCGAAGCCGCGGAAGAGGACCAAGAAGGAGAAGATCGACGAGGAGGAGGTGCTGATCATTAAGGACATCGAATTCGAGAGCAACGAGGCGGTGAAGTTCGATGTGTTTATTAATGATGATGCTGAGTCGCTCAGTAGGAAGGACAAATCCGAGTTTGCTGGGAGTTTTGTGCACGTGCCGCATAACCAGAAGACTGGGACGAAGAAAAAGACGAACTTAAAACTGGGGATCACGGACTTGTTGGAGGATTTGGGTGTGGAGGATGATAGCAGTGTGCTGGTGACGTTGGTGCCTAGGGTTTCGAACTCGCCTATCACCATTGGTGGGTTTAAGATCGAGTATTCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

601

Amino Acids

67.42

Weight (kDa)

6.33

Isoelectric Point (pI)

41.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	182 - 390	7.2e-37	Common central domain of tyrosinase
PPO1_DWL	PF12142	397 - 449	6.2e-21	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	470 - 598	8.9e-49	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g09060 FvH4_3g02000 FvH4_3g04660 FvH4_3g04670 FvH4_6g20570
malus_domestica	MD05G1319100.v1.1 MD05G1319300.v1.1 MD05G1319400.v1.1 MD05G1319500.v1.1 MD10G1298400.v1.1 MD10G1298500.v1.1 MD10G1298700.v1.1 MD10G1299100.v1.1 MD10G1299300.v1.1 MD10G1299400.v1.1
prunus_persica	Prupe.4G041400_v2.0.a1 Prupe.4G041900_v2.0.a1 Prupe.8G095500_v2.0.a1
pyrus_communis	pycom05g29510 pycom10g25180 pycom10g25190 pycom10g25200
rosa_chinensis	RchiOBHm_Chr5g0007711 RchiOBHm_Chr6g0264851 RchiOBHm_Chr6g0264871 RchiOBHm_Chr6g0264881
rosa_laevigata	RLG00000014231 RLG00000031477 RLG00000031478 RLG00000031480
rosa_multiflora	Rmu_co8436503.1_g000001 Rmu_sc0000546.1_g000043 Rmu_sc0000546.1_g000046 Rmu_sc0002934.1_g000059 Rmu_sc0005098.1_g000024 Rmu_sc0011744.1_g000007 Rmu_sc0011744.1_g000008
rosa_roxburghii	Rroxscaffold_175G00432040 Rroxscaffold_1G00068700 Rroxscaffold_1G00068740 Rroxscaffold_1G00068750 Rroxscaffold_7G00203160 Rroxscaffold_7G00203180
rosa_rugosa	Rorug04G0429200 Rorug04G0429400 Rorug04G0429600 Rorug06G0014200 Rorug06G0014300
rosa_samantha	Rh5AG057300 Rh5AG057400 Rh5AG058000 Rh5BG054300 Rh5CG064200 Rh5CG064300 Rh5CG064500 Rh5DG053500 Rh5DG053800 Rh6AG134800 Rh6AG134900 Rh6BG132400 Rh6CG131000 Rh6CG131100 Rh6DG118000 Rh6DG118100
rosa_wichuraiana	Rw5G005330 Rw5G005340 Rw5G006170 Rw6G011670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	189
AccB1I	GGYRCC	3 cut(s)	938, 1010, 1741
AccBSI	CCGCTC	1 cut(s)	1349
AccII	CGCG	2 cut(s)	544, 1462
AccIII	TCCGGA	2 cut(s)	427, 613
AclI	AACGTT	1 cut(s)	1158
AclWI	GGATC	5 cut(s)	438, 855, 1106, 1119, 1688
AcoI	YGGCCR	4 cut(s)	354, 489, 964, 1413
AcsI	RAATTY	1 cut(s)	1523
AcvI	CACGTG	1 cut(s)	1627
AfaI	GTAC	5 cut(s)	485, 671, 812, 920, 974
AfiI	CCNNNNNNNGG	7 cut(s)	277, 605, 841, 928, 1107, 1383, 1776
AgeI	ACCGGT	1 cut(s)	397
AgsI	TTSAA	2 cut(s)	499, 557
AjiI	CACGTC	1 cut(s)	877
AjnI	CCWGG	2 cut(s)	261, 641
AluBI	AGCT	7 cut(s)	506, 563, 703, 855, 1249, 1257, 1396
AluI	AGCT	7 cut(s)	506, 563, 703, 855, 1249, 1257, 1396
Alw21I	GWGCWC	1 cut(s)	1626
Alw26I	GTCTC	2 cut(s)	406, 978
Alw44I	GTGCAC	1 cut(s)	1622
AlwI	GGATC	5 cut(s)	438, 855, 1106, 1119, 1688
Aor13HI	TCCGGA	2 cut(s)	427, 613
ApaLI	GTGCAC	1 cut(s)	1622
ApoI	RAATTY	1 cut(s)	1523
ArsI	GACNNNNNNTTYG	4 cut(s)	704, 736, 1508, 1540
AseI	ATTAAT	1 cut(s)	1563
AsiGI	ACCGGT	1 cut(s)	397
AspA2I	CCTAGG	1 cut(s)	1745
AspLEI	GCGC	1 cut(s)	1185
AspS9I	GGNCC	4 cut(s)	279, 1144, 1189, 1470
AsuC2I	CCSGG	2 cut(s)	977, 1412
AsuHPI	GGTGA	7 cut(s)	355, 386, 1067, 1327, 1555, 1744, 1759
AsuII	TTCGAA	2 cut(s)	1427, 1754
AvaII	GGWCC	3 cut(s)	1144, 1189, 1470
AvrII	CCTAGG	1 cut(s)	1745
BaeGI	GKGCMC	2 cut(s)	50, 1626
BalI	TGGCCA	1 cut(s)	491
BamHI	GGATCC	1 cut(s)	1111
BanI	GGYRCC	3 cut(s)	938, 1010, 1741
BbrPI	CACGTG	1 cut(s)	1627
BbsI	GAAGAC	4 cut(s)	540, 1086, 1436, 1649
Bbv12I	GWGCWC	1 cut(s)	1626
BccI	CCATC	3 cut(s)	920, 960, 1050
BceAI	ACGGC	1 cut(s)	286
BciT130I	CCWGG	2 cut(s)	263, 643
BclI	TGATCA	3 cut(s)	63, 805, 1506
BcnI	CCSGG	2 cut(s)	977, 1412
BcoDI	GTCTC	2 cut(s)	406, 978
BfaI	CTAG	2 cut(s)	908, 1746
BglI	GCCNNNNNGGC	1 cut(s)	267
BglII	AGATCT	1 cut(s)	1291
BisI	GCNGC	4 cut(s)	268, 462, 1460, 1631
BlnI	CCTAGG	1 cut(s)	1745
BlsI	GCNGC	4 cut(s)	269, 463, 1461, 1632
BmcAI	AGTACT	1 cut(s)	485
Bme1390I	CCNGG	4 cut(s)	263, 643, 977, 1412
Bme18I	GGWCC	3 cut(s)	1144, 1189, 1470
BmgBI	CACGTC	1 cut(s)	877
BmgT120I	GGNCC	4 cut(s)	279, 1144, 1189, 1470
BmiI	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
BmrFI	CCNGG	4 cut(s)	263, 643, 977, 1412
BmrI	ACTGGG	2 cut(s)	1656, 1685
BmsI	GCATC	2 cut(s)	20, 1561
BmuI	ACTGGG	2 cut(s)	1656, 1685
BpiI	GAAGAC	4 cut(s)	540, 1086, 1436, 1649
Bpu14I	TTCGAA	2 cut(s)	1427, 1754
BpuEI	CTTGAG	1 cut(s)	492
BpuMI	CCSGG	2 cut(s)	977, 1412
BsaAI	YACGTR	2 cut(s)	149, 1627
BsaBI	GATNNNNATC	1 cut(s)	903
BsaI	GGTCTC	1 cut(s)	406
BsaJI	CCNNGG	8 cut(s)	42, 262, 694, 1171, 1325, 1377, 1460, 1745
BsaWI	WCCGGW	5 cut(s)	368, 397, 427, 613, 935
BsaXI	ACNNNNNCTCC	4 cut(s)	409, 439, 1082, 1112
Bsc4I	CCNNNNNNNGG	7 cut(s)	277, 605, 841, 928, 1107, 1383, 1776
Bse118I	RCCGGY	5 cut(s)	352, 397, 921, 1007, 1103
Bse1I	ACTGG	3 cut(s)	740, 1651, 1680
Bse3DI	GCAATG	3 cut(s)	141, 592, 1132
Bse8I	GATNNNNATC	1 cut(s)	903
BseAI	TCCGGA	2 cut(s)	427, 613
BseBI	CCWGG	2 cut(s)	263, 643
BseDI	CCNNGG	8 cut(s)	42, 262, 694, 1171, 1325, 1377, 1460, 1745
BseGI	GGATG	4 cut(s)	11, 624, 1038, 1720
BseJI	GATNNNNATC	1 cut(s)	903
BseLI	CCNNNNNNNGG	7 cut(s)	277, 605, 841, 928, 1107, 1383, 1776
BseMI	GCAATG	3 cut(s)	141, 592, 1132
BseMII	CTCAG	5 cut(s)	865, 1209, 1275, 1566, 1597
BseNI	ACTGG	3 cut(s)	740, 1651, 1680
BseRI	GAGGAG	2 cut(s)	1508, 1511
BseSI	GKGCMC	2 cut(s)	50, 1626
BseX3I	CGGCCG	1 cut(s)	354
BseYI	CCCAGC	2 cut(s)	1392, 1610
Bsh1236I	CGCG	2 cut(s)	544, 1462
Bsh1285I	CGRYCG	2 cut(s)	357, 1108
BshNI	GGYRCC	3 cut(s)	938, 1010, 1741
BshTI	ACCGGT	1 cut(s)	397
BsiEI	CGRYCG	2 cut(s)	357, 1108
BsiHKAI	GWGCWC	1 cut(s)	1626
BslFI	GGGAC	4 cut(s)	758, 1012, 1283, 1663
BslI	CCNNNNNNNGG	7 cut(s)	277, 605, 841, 928, 1107, 1383, 1776
BsmAI	GTCTC	2 cut(s)	406, 978
BsmFI	GGGAC	4 cut(s)	758, 1012, 1283, 1663
BsmI	GAATGC	1 cut(s)	392
Bso31I	GGTCTC	1 cut(s)	406
Bsp119I	TTCGAA	2 cut(s)	1427, 1754
Bsp1286I	GDGCHC	2 cut(s)	50, 1626
Bsp13I	TCCGGA	2 cut(s)	427, 613
Bsp19I	CCATGG	1 cut(s)	42
BspCNI	CTCAG	5 cut(s)	864, 1210, 1276, 1567, 1596
BspEI	TCCGGA	2 cut(s)	427, 613
BspFNI	CGCG	2 cut(s)	544, 1462
BspLI	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
BspPI	GGATC	5 cut(s)	438, 855, 1106, 1119, 1688
BspT104I	TTCGAA	2 cut(s)	1427, 1754
BspT107I	GGYRCC	3 cut(s)	938, 1010, 1741
BspTNI	GGTCTC	1 cut(s)	406
BsrBI	CCGCTC	1 cut(s)	1349
BsrDI	GCAATG	3 cut(s)	141, 592, 1132
BsrFI	RCCGGY	5 cut(s)	352, 397, 921, 1007, 1103
BsrI	ACTGG	3 cut(s)	740, 1651, 1680
BssAI	RCCGGY	5 cut(s)	352, 397, 921, 1007, 1103
BssECI	CCNNGG	8 cut(s)	42, 262, 694, 1171, 1325, 1377, 1460, 1745
BssT1I	CCWWGG	4 cut(s)	42, 694, 1377, 1745
Bst2UI	CCWGG	2 cut(s)	263, 643
Bst4CI	ACNGT	6 cut(s)	382, 575, 758, 860, 1025, 1438
Bst6I	CTCTTC	2 cut(s)	61, 1460
BstBAI	YACGTR	2 cut(s)	149, 1627
BstBI	TTCGAA	2 cut(s)	1427, 1754
BstC8I	GCNNGC	8 cut(s)	134, 324, 354, 459, 522, 701, 1009, 1181
BstDEI	CTNAG	5 cut(s)	851, 1218, 1284, 1575, 1583
BstDSI	CCRYGG	3 cut(s)	42, 1325, 1460
BstEII	GGTNACC	1 cut(s)	1055
BstF5I	GGATG	4 cut(s)	11, 624, 1038, 1720
BstFNI	CGCG	2 cut(s)	544, 1462
BstHHI	GCGC	1 cut(s)	1185
BstMAI	GTCTC	2 cut(s)	406, 978
BstMCI	CGRYCG	2 cut(s)	357, 1108
BstMWI	GCNNNNNNNGC	5 cut(s)	267, 454, 838, 1459, 1630
BstNI	CCWGG	2 cut(s)	263, 643
BstNSI	RCATGY	2 cut(s)	670, 1153
BstPI	GGTNACC	1 cut(s)	1055
BstSCI	CCNGG	4 cut(s)	261, 641, 975, 1410
BstSLI	GKGCMC	2 cut(s)	50, 1626
BstUI	CGCG	2 cut(s)	544, 1462
BstV2I	GAAGAC	4 cut(s)	540, 1086, 1436, 1649
BstX2I	RGATCY	3 cut(s)	847, 1111, 1291
BstXI	CCANNNNNNTGG	3 cut(s)	43, 322, 1646
BstYI	RGATCY	3 cut(s)	847, 1111, 1291
BstZI	CGGCCG	1 cut(s)	354
BtgI	CCRYGG	3 cut(s)	42, 1325, 1460
BtrI	CACGTC	1 cut(s)	877
BtsCI	GGATG	4 cut(s)	11, 624, 1038, 1720
BtsI	GCAGTG	2 cut(s)	443, 1729
BtsIMutI	CAGTG	3 cut(s)	443, 571, 1729
Cac8I	GCNNGC	8 cut(s)	134, 324, 354, 459, 522, 701, 1009, 1181
CfoI	GCGC	1 cut(s)	1185
Cfr10I	RCCGGY	5 cut(s)	352, 397, 921, 1007, 1103
Cfr13I	GGNCC	4 cut(s)	279, 1144, 1189, 1470
Cfr42I	CCGCGG	1 cut(s)	1463
CseI	GACGC	1 cut(s)	1223
Csp6I	GTAC	5 cut(s)	484, 670, 811, 919, 973
CspAI	ACCGGT	1 cut(s)	397
CviAII	CATG	8 cut(s)	43, 517, 550, 667, 916, 968, 1084, 1150
CviQI	GTAC	5 cut(s)	484, 670, 811, 919, 973
DdeI	CTNAG	5 cut(s)	851, 1218, 1284, 1575, 1583
EaeI	YGGCCR	4 cut(s)	354, 489, 964, 1413
EagI	CGGCCG	1 cut(s)	354
Eam1104I	CTCTTC	2 cut(s)	61, 1460
EarI	CTCTTC	2 cut(s)	61, 1460
EciI	GGCGGA	1 cut(s)	1090
EclXI	CGGCCG	1 cut(s)	354
Eco130I	CCWWGG	4 cut(s)	42, 694, 1377, 1745
Eco147I	AGGCCT	1 cut(s)	457
Eco31I	GGTCTC	1 cut(s)	406
Eco47I	GGWCC	3 cut(s)	1144, 1189, 1470
Eco52I	CGGCCG	1 cut(s)	354
Eco72I	CACGTG	1 cut(s)	1627
Eco91I	GGTNACC	1 cut(s)	1055
EcoO109I	RGGNCCY	1 cut(s)	1189
EcoO65I	GGTNACC	1 cut(s)	1055
EcoRI	GAATTC	1 cut(s)	1523
EcoRII	CCWGG	2 cut(s)	261, 641
EcoT14I	CCWWGG	4 cut(s)	42, 694, 1377, 1745
ErhI	CCWWGG	4 cut(s)	42, 694, 1377, 1745
FaeI	CATG	8 cut(s)	46, 520, 553, 670, 919, 971, 1087, 1153
FalI	AAGNNNNNCTT	2 cut(s)	1652, 1684
FaqI	GGGAC	4 cut(s)	758, 1012, 1283, 1663
FatI	CATG	8 cut(s)	42, 516, 549, 666, 915, 967, 1083, 1149
FauI	CCCGC	3 cut(s)	28, 327, 549
FbaI	TGATCA	3 cut(s)	63, 805, 1506
Fnu4HI	GCNGC	4 cut(s)	268, 462, 1460, 1631
FokI	GGATG	3 cut(s)	611, 1025, 1727
Fsp4HI	GCNGC	4 cut(s)	268, 462, 1460, 1631
FspBI	CTAG	2 cut(s)	908, 1746
GlaI	GCGC	1 cut(s)	1184
GluI	GCNGC	4 cut(s)	268, 462, 1460, 1631
GsaI	CCCAGC	2 cut(s)	1396, 1614
HgaI	GACGC	1 cut(s)	1223
HhaI	GCGC	1 cut(s)	1185
Hin1II	CATG	8 cut(s)	46, 520, 553, 670, 919, 971, 1087, 1153
Hin6I	GCGC	1 cut(s)	1183
HinP1I	GCGC	1 cut(s)	1183
HinfI	GANTC	5 cut(s)	690, 746, 904, 1424, 1577
HphI	GGTGA	7 cut(s)	355, 386, 1067, 1327, 1555, 1744, 1759
Hpy166II	GTNNAC	6 cut(s)	395, 571, 880, 1144, 1335, 1624
Hpy188I	TCNGA	2 cut(s)	1175, 1603
Hpy8I	GTNNAC	6 cut(s)	395, 571, 880, 1144, 1335, 1624
Hpy99I	CGWCG	2 cut(s)	1438, 1493
HpyAV	CCTTC	6 cut(s)	91, 100, 127, 430, 1471, 1585
HpyCH4III	ACNGT	6 cut(s)	382, 575, 758, 860, 1025, 1438
HpyCH4IV	ACGT	7 cut(s)	148, 876, 1140, 1158, 1418, 1626, 1736
HpyCH4V	TGCA	6 cut(s)	11, 146, 168, 585, 1137, 1624
HpyF10VI	GCNNNNNNNGC	5 cut(s)	267, 454, 838, 1459, 1630
HpyF3I	CTNAG	5 cut(s)	851, 1218, 1284, 1575, 1583
HpySE526I	ACGT	7 cut(s)	148, 876, 1140, 1158, 1418, 1626, 1736
Hsp92II	CATG	8 cut(s)	46, 520, 553, 670, 919, 971, 1087, 1153
HspAI	GCGC	1 cut(s)	1183
Kpn2I	TCCGGA	2 cut(s)	427, 613
KroI	GCCGGC	2 cut(s)	352, 1007
KroNI	GCCGGC	2 cut(s)	354, 1009
Ksp22I	TGATCA	3 cut(s)	63, 805, 1506
KspI	CCGCGG	1 cut(s)	1463
LmnI	GCTCC	1 cut(s)	331
LweI	GCATC	2 cut(s)	20, 1561
MaeI	CTAG	2 cut(s)	908, 1746
MaeII	ACGT	7 cut(s)	148, 876, 1140, 1158, 1418, 1626, 1736
MaeIII	GTNAC	4 cut(s)	472, 1055, 1088, 1732
MbiI	CCGCTC	1 cut(s)	1349
MflI	RGATCY	3 cut(s)	847, 1111, 1291
MhlI	GDGCHC	2 cut(s)	50, 1626
MlsI	TGGCCA	1 cut(s)	491
MluCI	AATT	8 cut(s)	76, 184, 220, 233, 255, 739, 765, 1523
MluNI	TGGCCA	1 cut(s)	491
MlyI	GAGTC	2 cut(s)	740, 1586
MmeI	TCCRAC	1 cut(s)	1674
Mox20I	TGGCCA	1 cut(s)	491
MroI	TCCGGA	2 cut(s)	427, 613
MroNI	GCCGGC	2 cut(s)	352, 1007
MscI	TGGCCA	1 cut(s)	491
MseI	TTAA	4 cut(s)	1512, 1563, 1670, 1782
MslI	CAYNNNNRTG	1 cut(s)	41
Msp20I	TGGCCA	1 cut(s)	491
MspA1I	CMGCKG	3 cut(s)	322, 1396, 1462
MspR9I	CCNGG	4 cut(s)	263, 643, 977, 1412
Mva1269I	GAATGC	1 cut(s)	392
MvaI	CCWGG	2 cut(s)	263, 643
MvnI	CGCG	2 cut(s)	544, 1462
MwoI	GCNNNNNNNGC	5 cut(s)	267, 454, 838, 1459, 1630
NaeI	GCCGGC	2 cut(s)	354, 1009
NciI	CCSGG	2 cut(s)	977, 1412
NcoI	CCATGG	1 cut(s)	42
NgoMIV	GCCGGC	2 cut(s)	352, 1007
NlaIII	CATG	8 cut(s)	46, 520, 553, 670, 919, 971, 1087, 1153
NlaIV	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
NmeAIII	GCCGAG	1 cut(s)	1270
NmuCI	GTSAC	3 cut(s)	472, 1055, 1732
NspI	RCATGY	2 cut(s)	670, 1153
NspV	TTCGAA	2 cut(s)	1427, 1754
PceI	AGGCCT	1 cut(s)	457
PcsI	WCGNNNNNNNCGW	3 cut(s)	864, 1424, 1533
PctI	GAATGC	1 cut(s)	392
PdiI	GCCGGC	2 cut(s)	354, 1009
PfeI	GAWTC	3 cut(s)	690, 904, 1424
PinAI	ACCGGT	1 cut(s)	397
PkrI	GCNGC	4 cut(s)	269, 463, 1461, 1632
PleI	GAGTC	2 cut(s)	740, 1585
PmaCI	CACGTG	1 cut(s)	1627
PmlI	CACGTG	1 cut(s)	1627
PpsI	GAGTC	2 cut(s)	740, 1585
Ppu21I	YACGTR	2 cut(s)	149, 1627
PpuMI	RGGWCCY	1 cut(s)	1189
PshBI	ATTAAT	1 cut(s)	1563
PsiI	TTATAA	1 cut(s)	189
Psp1406I	AACGTT	1 cut(s)	1158
Psp5II	RGGWCCY	1 cut(s)	1189
Psp6I	CCWGG	2 cut(s)	261, 641
PspCI	CACGTG	1 cut(s)	1627
PspEI	GGTNACC	1 cut(s)	1055
PspFI	CCCAGC	2 cut(s)	1392, 1610
PspGI	CCWGG	2 cut(s)	261, 641
PspN4I	GGNNCC	8 cut(s)	280, 327, 865, 940, 1012, 1113, 1191, 1743
PspPI	GGNCC	4 cut(s)	279, 1144, 1189, 1470
PspPPI	RGGWCCY	1 cut(s)	1189
PsuI	RGATCY	3 cut(s)	847, 1111, 1291
PvuII	CAGCTG	1 cut(s)	1396
RsaI	GTAC	5 cut(s)	485, 671, 812, 920, 974
RsaNI	GTAC	5 cut(s)	484, 670, 811, 919, 973
RseI	CAYNNNNRTG	1 cut(s)	41
SacII	CCGCGG	1 cut(s)	1463
SaqAI	TTAA	4 cut(s)	1512, 1563, 1670, 1782
SatI	GCNGC	4 cut(s)	268, 462, 1460, 1631
Sau96I	GGNCC	4 cut(s)	279, 1144, 1189, 1470
ScaI	AGTACT	1 cut(s)	485
SchI	GAGTC	2 cut(s)	740, 1586
ScrFI	CCNGG	4 cut(s)	263, 643, 977, 1412
SduI	GDGCHC	2 cut(s)	50, 1626
SfaNI	GCATC	2 cut(s)	20, 1561
SfiI	GGCCNNNNNGGCC	1 cut(s)	267
Sfr303I	CCGCGG	1 cut(s)	1463
SfuI	TTCGAA	2 cut(s)	1427, 1754
SgrBI	CCGCGG	1 cut(s)	1463
SinI	GGWCC	3 cut(s)	1144, 1189, 1470
SmiMI	CAYNNNNRTG	1 cut(s)	41
SmlI	CTYRAG	1 cut(s)	507
SmoI	CTYRAG	1 cut(s)	507
Sse9I	AATT	8 cut(s)	76, 184, 220, 233, 255, 739, 765, 1523
SseBI	AGGCCT	1 cut(s)	457
SspMI	CTAG	2 cut(s)	908, 1746
StuI	AGGCCT	1 cut(s)	457
StyD4I	CCNGG	4 cut(s)	261, 641, 975, 1410
StyI	CCWWGG	4 cut(s)	42, 694, 1377, 1745
TaaI	ACNGT	6 cut(s)	382, 575, 758, 860, 1025, 1438
TaiI	ACGT	7 cut(s)	151, 879, 1143, 1161, 1421, 1629, 1739
TaqI	TCGA	7 cut(s)	1427, 1488, 1521, 1527, 1551, 1754, 1788
TaqII	GACCGA	1 cut(s)	423
TasI	AATT	8 cut(s)	76, 184, 220, 233, 255, 739, 765, 1523
TatI	WGTACW	2 cut(s)	483, 669
TauI	GCSGC	4 cut(s)	270, 464, 1462, 1633
TfiI	GAWTC	3 cut(s)	690, 904, 1424
Tru1I	TTAA	4 cut(s)	1512, 1563, 1670, 1782
Tru9I	TTAA	4 cut(s)	1512, 1563, 1670, 1782
TscAI	CASTG	3 cut(s)	450, 578, 1729
TseFI	GTSAC	3 cut(s)	472, 1055, 1732
Tsp45I	GTSAC	3 cut(s)	472, 1055, 1732
TspDTI	ATGAA	1 cut(s)	1251
TspGWI	ACGGA	3 cut(s)	491, 1314, 1700
TspRI	CASTG	3 cut(s)	450, 578, 1729
VneI	GTGCAC	1 cut(s)	1622
VpaK11BI	GGWCC	3 cut(s)	1144, 1189, 1470
VspI	ATTAAT	1 cut(s)	1563
XapI	RAATTY	1 cut(s)	1523
XceI	RCATGY	2 cut(s)	670, 1153
XcmI	CCANNNNNNNNNTGG	2 cut(s)	40, 738
XmaJI	CCTAGG	1 cut(s)	1745
XspI	CTAG	2 cut(s)	908, 1746
ZrmI	AGTACT	1 cut(s)	485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.