Rmu_sc0000546.1_g000043

Common central domain of tyrosinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000546.1
OG0000836
Physical Location & Seq
Reverse (-)
187363 .. 188949
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000546.1_g000043.1.cds

Sequence Viewer

Length: 1587 bp
atggacacagtcctaggcgtattaggaatagttgactcagatgagtcttcaggaggctacaagattgataaagaggacctttcagtaaggttcaacttaacaagttgtcactcatcttatggaaggccagatcttcttgtctattgttgtcctccaagaagtatagcagaagaacccgtcattgattttcaatttccagatccttcaacccctctaagaattcggaagccagcacaccgtgttgatcgtggcttcattgccaagtacaaaaaggctctcttaatcatgaagtcacttccctacagtgaccctcggagttttcgtcgtcaagctgatatgcattgcatcttctgcactggtgcttatgaccagccacattcccaccgccctctcgacatccatcggaaatggttctttttcccatggcatcggatgctgcttcacttccacgaacgcatcgtcggaagcctaattggtgatgacacatttgctctgccattttggaattgggactcccctgatggaatggcaatgcctgaatggtacatgcattcaccttttttcgacaatcagcgggacgtttctcactttccaccaaaagttagtgatttaaactacgattacgagaagaccgatgagaatatggaaaacaaagagcggatgcacttgaacatggtgttcatgtataaccagatggtgtctggagcaaagaagccagagctgttcatggggtgcccttataagaatagtgaggagggtgcttgtgatggccctggtacaatagagaatgctcctcacaacacaatgcatacatgggttggtagcaatttcaatgtcaggagggaagacatgggagcattttattctgcagctagggatcctagtttctatgcacaccactcgaacttggaccgactttgggaggtttggaagcaaattcacaaggtcgacttgcaaacatttgatcctgattggctcaactctagtttctatttccatgatgagaattcacagcttgtgagaatcaaagttcgtgatgttttggatactaaaaagctgaggtatgtttatgaggaggttgatcttccatggctgaataagcgtccaaagcccaactctattccatccaaaattgccaactatgcactgaaaataagagaaaacagaaccattttgtctgcagaattaggaccaggtggtcggaatcttgagagctccataacagtgaaggtgggcaggtctaaatttaacagaagaaaccaagagaaagatgaggaggaagagattttggttgttcatggaattgctgtgagaattgatgagtatgttaagtttgatgtgtatataaatgtagtcgatgagagcaaaatcgagccgggtttcagggaatttgccggaacctttgttcacatacctcatggaaagatggatgatgacagaaagaccaatctgaagtttggtatttcagaagtgcttgaggatttggaggcagatagagatgagagcatttgggtgacattgctacccatgacgaagtcatgcactaacacaacaattgatgggattcggattgaatacatgagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110
Pfam Domains
Protein Families
PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

528

Amino Acids

61.56

Weight (kDa)

5.98

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 741
AasI GACNNNNNNGTC 1 cut(s) 1197
Acc36I ACCTGC 1 cut(s) 1227
AccB1I GGYRCC 1 cut(s) 732
AccBSI CCGCTC 1 cut(s) 658
AccI GTMKAC 1 cut(s) 948
AciI CCGC 3 cut(s) 385, 574, 658
AclWI GGATC 4 cut(s) 194, 872, 885, 959
AcsI RAATTY 5 cut(s) 219, 936, 1006, 1244, 1388
AcuI CTGAAG 2 cut(s) 33, 1472
AdeI CACNNNGTG 1 cut(s) 239
AfaI GTAC 3 cut(s) 266, 545, 778
AfiI CCNNNNNNNGG 1 cut(s) 919
AgsI TTSAA 6 cut(s) 94, 191, 207, 670, 832, 1574
AjnI CCWGG 2 cut(s) 772, 1192
AloI GAACNNNNNNTCC 2 cut(s) 1389, 1421
AluBI AGCT 6 cut(s) 332, 721, 872, 1015, 1057, 1215
AluI AGCT 6 cut(s) 332, 721, 872, 1015, 1057, 1215
Alw21I GWGCWC 1 cut(s) 1217
AlwI GGATC 4 cut(s) 194, 872, 885, 959
AoxI GGCC 2 cut(s) 125, 769
ApeKI GCWGC 2 cut(s) 436, 869
ApoI RAATTY 5 cut(s) 219, 936, 1006, 1244, 1388
ArsI GACNNNNNNTTYG 2 cut(s) 444, 476
Asp700I GAANNNNTTC 1 cut(s) 410
AspA2I CCTAGG 1 cut(s) 13
AspS9I GGNCC 4 cut(s) 76, 770, 910, 1190
AsuC2I CCSGG 1 cut(s) 1377
AsuHPI GGTGA 3 cut(s) 488, 546, 1525
AvaII GGWCC 3 cut(s) 76, 910, 1190
AvrII CCTAGG 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 737
BamHI GGATCC 1 cut(s) 877
BanI GGYRCC 1 cut(s) 732
BanII GRGCYC 1 cut(s) 1217
BbsI GAAGAC 3 cut(s) 39, 635, 852
Bbv12I GWGCWC 1 cut(s) 1217
BbvCI CCTCAGC 1 cut(s) 1058
BbvI GCAGC 2 cut(s) 423, 881
BccI CCATC 7 cut(s) 408, 515, 688, 761, 1132, 1420, 1553
BcgI CGANNNNNNTGC 2 cut(s) 882, 916
BciT130I CCWGG 2 cut(s) 774, 1194
BciVI GTATCC 1 cut(s) 1039
BcnI CCSGG 1 cut(s) 1377
BfaI CTAG 4 cut(s) 14, 873, 882, 984
BfmI CTRYAG 3 cut(s) 301, 867, 1179
BfuAI ACCTGC 1 cut(s) 1227
BfuI GTATCC 1 cut(s) 1039
BglII AGATCT 1 cut(s) 130
BisI GCNGC 2 cut(s) 437, 870
BlnI CCTAGG 1 cut(s) 13
BlsI GCNGC 2 cut(s) 438, 871
Bme1390I CCNGG 3 cut(s) 774, 1194, 1377
Bme18I GGWCC 3 cut(s) 76, 910, 1190
BmgT120I GGNCC 4 cut(s) 76, 770, 910, 1190
BmiI GGNNCC 3 cut(s) 734, 879, 1399
BmrFI CCNGG 3 cut(s) 774, 1194, 1377
BmsI GCATC 5 cut(s) 354, 423, 436, 465, 651
BpiI GAAGAC 3 cut(s) 39, 635, 852
BpmI CTGGAG 1 cut(s) 723
Bpu10I CCTNAGC 1 cut(s) 1058
BpuEI CTTGAG 2 cut(s) 1229, 1496
BpuMI CCSGG 1 cut(s) 1377
BsaJI CCNNGG 5 cut(s) 13, 311, 422, 772, 1088
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 1 cut(s) 919
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 4 cut(s) 255, 340, 537, 1517
BseBI CCWGG 2 cut(s) 774, 1194
BseDI CCNNGG 5 cut(s) 13, 311, 422, 772, 1088
BseGI GGATG 5 cut(s) 396, 438, 666, 1124, 1435
BseLI CCNNNNNNNGG 1 cut(s) 919
BseMI GCAATG 4 cut(s) 255, 340, 537, 1517
BseMII CTCAG 2 cut(s) 51, 1049
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 4 cut(s) 767, 783, 1088, 1289
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 2 cut(s) 423, 881
BsgI GTGCAG 1 cut(s) 337
BshFI GGCC 2 cut(s) 127, 771
BshNI GGYRCC 1 cut(s) 732
BsiHKAI GWGCWC 1 cut(s) 1217
BsiSI CCGG 2 cut(s) 1376, 1395
BslFI GGGAC 2 cut(s) 524, 590
BslI CCNNNNNNNGG 1 cut(s) 919
BsmFI GGGAC 2 cut(s) 524, 590
BsmI GAATGC 2 cut(s) 550, 793
BsnI GGCC 2 cut(s) 127, 771
Bsp1286I GDGCHC 2 cut(s) 737, 1217
Bsp143I GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
Bsp19I CCATGG 2 cut(s) 422, 1088
BspACI CCGC 3 cut(s) 385, 574, 658
BspANI GGCC 2 cut(s) 127, 771
BspCNI CTCAG 2 cut(s) 50, 1050
BspHI TCATGA 1 cut(s) 285
BspLI GGNNCC 3 cut(s) 734, 879, 1399
BspMAI CTGCAG 2 cut(s) 871, 1183
BspMI ACCTGC 1 cut(s) 1227
BspPI GGATC 4 cut(s) 194, 872, 885, 959
BspT107I GGYRCC 1 cut(s) 732
BsrBI CCGCTC 1 cut(s) 658
BsrDI GCAATG 4 cut(s) 255, 340, 537, 1517
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 5 cut(s) 13, 311, 422, 772, 1088
BssMI GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
BssT1I CCWWGG 3 cut(s) 13, 422, 1088
Bst2UI CCWGG 2 cut(s) 774, 1194
Bst4CI ACNGT 4 cut(s) 10, 239, 305, 1225
Bst6I CTCTTC 1 cut(s) 1275
BstAPI GCANNNNNTGC 2 cut(s) 351, 433
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 3 cut(s) 37, 215, 1058
BstDSI CCRYGG 2 cut(s) 422, 1088
BstF5I GGATG 5 cut(s) 396, 438, 666, 1124, 1435
BstKTI GATC 6 cut(s) 133, 202, 247, 880, 967, 1084
BstMBI GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
BstMWI GCNNNNNNNGC 5 cut(s) 351, 433, 1099, 1108, 1142
BstNI CCWGG 2 cut(s) 774, 1194
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 3 cut(s) 772, 1192, 1375
BstSFI CTRYAG 3 cut(s) 301, 867, 1179
BstSLI GKGCMC 1 cut(s) 737
BstV1I GCAGC 2 cut(s) 423, 881
BstV2I GAAGAC 3 cut(s) 39, 635, 852
BstX2I RGATCY 3 cut(s) 130, 199, 877
BstYI RGATCY 3 cut(s) 130, 199, 877
BsuI GTATCC 1 cut(s) 1039
BsuRI GGCC 2 cut(s) 127, 771
BtgI CCRYGG 2 cut(s) 422, 1088
BtsCI GGATG 5 cut(s) 396, 438, 666, 1124, 1435
BtsIMutI CAGTG 4 cut(s) 310, 354, 1145, 1230
BveI ACCTGC 1 cut(s) 1227
Cac8I GCNNGC 1 cut(s) 231
CciI TCATGA 1 cut(s) 285
Cfr13I GGNCC 4 cut(s) 76, 770, 910, 1190
CseI GACGC 1 cut(s) 1091
CsiI ACCWGGT 1 cut(s) 1192
Csp6I GTAC 3 cut(s) 265, 544, 777
CviQI GTAC 3 cut(s) 265, 544, 777
DdeI CTNAG 3 cut(s) 37, 215, 1058
DpnI GATC 6 cut(s) 132, 201, 246, 879, 966, 1083
DpnII GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
DraI TTTAAA 1 cut(s) 612
DraIII CACNNNGTG 1 cut(s) 239
DrdI GACNNNNNNGTC 1 cut(s) 1197
DseDI GACNNNNNNGTC 1 cut(s) 1197
Eam1104I CTCTTC 1 cut(s) 1275
EarI CTCTTC 1 cut(s) 1275
Ecl136II GAGCTC 1 cut(s) 1215
Eco130I CCWWGG 3 cut(s) 13, 422, 1088
Eco24I GRGCYC 1 cut(s) 1217
Eco47I GGWCC 3 cut(s) 76, 910, 1190
Eco53kI GAGCTC 1 cut(s) 1215
Eco57I CTGAAG 2 cut(s) 33, 1472
EcoICRI GAGCTC 1 cut(s) 1215
EcoO109I RGGNCCY 1 cut(s) 76
EcoRI GAATTC 2 cut(s) 219, 1006
EcoRII CCWGG 2 cut(s) 772, 1192
EcoT14I CCWWGG 3 cut(s) 13, 422, 1088
EcoT22I ATGCAT 3 cut(s) 342, 552, 810
EcoT38I GRGCYC 1 cut(s) 1217
ErhI CCWWGG 3 cut(s) 13, 422, 1088
FalI AAGNNNNNCTT 6 cut(s) 63, 95, 263, 295, 935, 967
FaqI GGGAC 2 cut(s) 524, 590
FauI CCCGC 1 cut(s) 567
FblI GTMKAC 1 cut(s) 948
Fnu4HI GCNGC 2 cut(s) 437, 870
FokI GGATG 5 cut(s) 383, 445, 673, 1111, 1442
FriOI GRGCYC 1 cut(s) 1217
Fsp4HI GCNGC 2 cut(s) 437, 870
FspBI CTAG 4 cut(s) 14, 873, 882, 984
GluI GCNGC 2 cut(s) 437, 870
GsuI CTGGAG 1 cut(s) 723
HaeIII GGCC 2 cut(s) 127, 771
HapII CCGG 2 cut(s) 1376, 1395
HgaI GACGC 1 cut(s) 1091
HincII GTYRAC 2 cut(s) 34, 949
HindII GTYRAC 2 cut(s) 34, 949
HinfI GANTC 6 cut(s) 35, 44, 512, 1023, 1204, 1564
HpaII CCGG 2 cut(s) 1376, 1395
HphI GGTGA 3 cut(s) 488, 546, 1525
Hpy166II GTNNAC 3 cut(s) 34, 949, 1408
Hpy188III TCNNGA 9 cut(s) 51, 197, 286, 392, 702, 838, 968, 1034, 1208
Hpy8I GTNNAC 3 cut(s) 34, 949, 1408
Hpy99I CGWCG 2 cut(s) 327, 464
HpyAV CCTTC 3 cut(s) 117, 213, 1222
HpyCH4III ACNGT 4 cut(s) 10, 239, 305, 1225
HpyCH4IV ACGT 1 cut(s) 579
HpyF10VI GCNNNNNNNGC 5 cut(s) 351, 433, 1099, 1108, 1142
HpyF3I CTNAG 3 cut(s) 37, 215, 1058
HpySE526I ACGT 1 cut(s) 579
Kzo9I GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
LmnI GCTCC 4 cut(s) 704, 796, 854, 1220
Lsp1109I GCAGC 2 cut(s) 423, 881
LweI GCATC 5 cut(s) 354, 423, 436, 465, 651
MabI ACCWGGT 1 cut(s) 1192
MaeI CTAG 4 cut(s) 14, 873, 882, 984
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 4 cut(s) 107, 291, 305, 1513
MalI GATC 6 cut(s) 132, 201, 246, 879, 966, 1083
MbiI CCGCTC 1 cut(s) 658
MboI GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
MboII GAAGA 9 cut(s) 39, 125, 182, 340, 640, 857, 1076, 1266, 1292
MfeI CAATTG 1 cut(s) 1554
MflI RGATCY 3 cut(s) 130, 199, 877
MhlI GDGCHC 2 cut(s) 737, 1217
MlyI GAGTC 3 cut(s) 29, 53, 506
MmeI TCCRAC 2 cut(s) 442, 1181
Mph1103I ATGCAT 3 cut(s) 342, 552, 810
MroXI GAANNNNTTC 1 cut(s) 410
MseI TTAA 5 cut(s) 98, 281, 611, 1248, 1329
MslI CAYNNNNRTG 2 cut(s) 1223, 1511
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 2 cut(s) 1376, 1395
MspR9I CCNGG 3 cut(s) 774, 1194, 1377
MunI CAATTG 1 cut(s) 1554
Mva1269I GAATGC 2 cut(s) 550, 793
MvaI CCWGG 2 cut(s) 774, 1194
MwoI GCNNNNNNNGC 5 cut(s) 351, 433, 1099, 1108, 1142
NciI CCSGG 1 cut(s) 1377
NcoI CCATGG 2 cut(s) 422, 1088
NdeII GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
NlaIV GGNNCC 3 cut(s) 734, 879, 1399
NmuCI GTSAC 4 cut(s) 107, 291, 305, 1513
NsiI ATGCAT 3 cut(s) 342, 552, 810
NspI RCATGY 1 cut(s) 550
PagI TCATGA 1 cut(s) 285
PcsI WCGNNNNNNNCGW 3 cut(s) 319, 456, 630
PctI GAATGC 2 cut(s) 550, 793
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 3 cut(s) 1023, 1204, 1564
PflFI GACNNNGTC 2 cut(s) 8, 1534
PkrI GCNGC 2 cut(s) 438, 871
PleI GAGTC 3 cut(s) 29, 52, 506
PpsI GAGTC 3 cut(s) 29, 52, 506
PpuMI RGGWCCY 1 cut(s) 76
PsiI TTATAA 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 1217
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 772, 1192
PspGI CCWGG 2 cut(s) 772, 1192
PspN4I GGNNCC 3 cut(s) 734, 879, 1399
PspPI GGNCC 4 cut(s) 76, 770, 910, 1190
PspPPI RGGWCCY 1 cut(s) 76
PstI CTGCAG 2 cut(s) 871, 1183
PsuI RGATCY 3 cut(s) 130, 199, 877
PsyI GACNNNGTC 2 cut(s) 8, 1534
RsaI GTAC 3 cut(s) 266, 545, 778
RsaNI GTAC 3 cut(s) 265, 544, 777
RseI CAYNNNNRTG 2 cut(s) 1223, 1511
SacI GAGCTC 1 cut(s) 1217
SalI GTCGAC 1 cut(s) 947
SaqAI TTAA 5 cut(s) 98, 281, 611, 1248, 1329
SatI GCNGC 2 cut(s) 437, 870
Sau3AI GATC 6 cut(s) 130, 199, 244, 877, 964, 1081
Sau96I GGNCC 4 cut(s) 76, 770, 910, 1190
SchI GAGTC 3 cut(s) 29, 53, 506
ScrFI CCNGG 3 cut(s) 774, 1194, 1377
SduI GDGCHC 2 cut(s) 737, 1217
SexAI ACCWGGT 1 cut(s) 1192
SfaNI GCATC 5 cut(s) 354, 423, 436, 465, 651
SfcI CTRYAG 3 cut(s) 301, 867, 1179
SinI GGWCC 3 cut(s) 76, 910, 1190
SmiMI CAYNNNNRTG 2 cut(s) 1223, 1511
SmlI CTYRAG 2 cut(s) 1208, 1475
SmoI CTYRAG 2 cut(s) 1208, 1475
SsiI CCGC 3 cut(s) 385, 574, 658
SspMI CTAG 4 cut(s) 14, 873, 882, 984
SstI GAGCTC 1 cut(s) 1217
StyD4I CCNGG 3 cut(s) 772, 1192, 1375
StyI CCWWGG 3 cut(s) 13, 422, 1088
TaaI ACNGT 4 cut(s) 10, 239, 305, 1225
TaiI ACGT 1 cut(s) 582
TaqI TCGA 6 cut(s) 393, 564, 902, 948, 1356, 1371
TaqII GACCGA 2 cut(s) 647, 927
TatI WGTACW 1 cut(s) 264
TfiI GAWTC 3 cut(s) 1023, 1204, 1564
Tru1I TTAA 5 cut(s) 98, 281, 611, 1248, 1329
Tru9I TTAA 5 cut(s) 98, 281, 611, 1248, 1329
TscAI CASTG 4 cut(s) 310, 361, 1152, 1230
TseFI GTSAC 4 cut(s) 107, 291, 305, 1513
TseI GCWGC 2 cut(s) 436, 869
Tsp45I GTSAC 4 cut(s) 107, 291, 305, 1513
TspDTI ATGAA 5 cut(s) 244, 302, 670, 715, 1286
TspRI CASTG 4 cut(s) 310, 361, 1152, 1230
Tth111I GACNNNGTC 2 cut(s) 8, 1534
VpaK11BI GGWCC 3 cut(s) 76, 910, 1190
XapI RAATTY 5 cut(s) 219, 936, 1006, 1244, 1388
XceI RCATGY 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 2 cut(s) 504, 698
XmaJI CCTAGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 948
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 4 cut(s) 14, 873, 882, 984
Zsp2I ATGCAT 3 cut(s) 342, 552, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.