FvH4_3g10610

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004224
Physical Location & Seq
Forward (+)
6314941 .. 6323553
8613 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10610.t1

Sequence Viewer

Length: 2460 bp
ATGGATCGTGAACGGAGCTTTGGTCTCCGATTGCAAATACATGCACTTCATCGGAGCTCCGCACCGTACCGCTCACCCTCAACTAATACCTCTTCTCCGCTGCGCTCAGCCGCCGCTCTCAGCAGCCGCATCTCTCTCTCCCTCTCCATCTCCGGCTTGGTCCTTCTTCTTCATCGCCGTCCAAGTCTTCGATCGTCTTCTTCTTTTCAAGCAGTCAAGAATGCAGTACAATGTGGTAATGTGGAGGATGCAATCGATAAGGTGAATGACTTAAACCTGGAGAAAGAGAAGCAAAGTAACAATGAAGCTAATGGGACGATACAATTTCCGGTAGTAGATGAGAAACTAAGTGTAGCAGATGATGATCAGAAGGTCCATGTGGAAATGAAGCAAGAGTGCTTGGCAGCAGTAGCGGGTACAAGTGCGACTCTAATCAAGAAAGAGGAGGTGATCGATTCTGAGGACTACTCCAACGCCTACATCAACAATATTGATGCTGACGAGTTCCTTAATCTGCTCAATGAAGAAGAACCTTTGAGTTGGGAGGTGGACAGATCGGAACTGGGAGTGCAACAAGATCGATGCGACACCTCATGGAGGTTGGTGGTCCAGCCGGAGTTGGTGGTTCGACGGTGGTGGCAAGGCGGTACTCCTGTAGTGGTGGTGGTTTGGCGGTGTAATCCCGCACTGGTGGTGGAAGCCGGATCTCTAAGGTGCTGCTCACCCGTGCCGGCGGAGGTCGTCGGCTCTCAAAAGAAACCCTCAAGTCTTATGCCCTTGGTACATGGGAAAATGCTTCTCATGGAACTACTCTTATTAGTTTCATTAGCAGCAGCTGCAACGGTATTGCTAGCAGCAGAAGAAGCTGATAAACCCCTCCCACTGCCTCAAGCAAAGCCAAACTGCCCAGATCGCTGTGGTAACCTTACAATTCCATATCCATTTGGCATCGGCGACCGCTGTTACATGTCACCGATGGGACAGGAAACCCGATTTAGTCTCGTCTGTGATAATTCCACAACAGCCCCATCGTTGAACTGGACGTCCAGCAATGTCCGTATTACTGGATTTGTCGTTGCTCAGGATATCAACGAGTGTTTGAATGAAACTAATCCATGCCGGAACGGAACGTGCGAAAATTTAAGTGGAACTTACTCTTGTAAATGTGATGAAGGGTTCGAAAATGATGGCCAAGGGAAATGCGTTGCTCGCCCGGCAAACAACACTACTCCCCTCAAAATTGCATTGGGTATTTGTATAGGCTTCTTTGTTCTATTAGTCGTAAGTTTTGTGTTATATCGTGAATACAAAAGAAGACAGTTCAACAAGATAAGAAAAAAGTACTTTGATGCGAACGGCGGGCCAATATTGGAAGCAAAACTAAAGCAATTGGCTAGTCAAAAAGATTCCTCACTTGCGGCCCAAATCTTTTCTGCAGAAGATCTCAAGAAAGCAACAAAGGATTACAATGAAAATGAAAAAATTGGTGAAGGTGGCTATGAAATAGTCTACAGAGGCGTATTTGATAAAAGAGTGGTTGCTGTGAAGAAGTCCAAAATGACTGTTCAGAAAAGTGATCAGTTTATTAACGAGGTCATTGTTCTTTCTCAAATCAGTCATAGAAATGTGGTGAGGCTGCTAGGCTGTTGTTTGGAGACAAAAACGCCTATCCTGGTTTACGAGTATGTTGGTAATGGAACTCTCTGCGATCATATTCATAAGAAGAAAGGAGAACTACCACTTTCATTTGCACAACGATTGAAGATAGCAGCAGAGACTGCAACCGCACTTTCATACTTGCATCACTCGACCACCACGCAAATTGTTCATCGAGATGTCAAGGCAACAAATATACTGTTGGATGAGAAACTTACGGCCAAAGTGTCGGATTTCGGGGCTTCGAAACTGGTTCCTGACGACGAAACTCAACTTCCAACTTTAGTGCAGGGGACAATGGGATACTTGGATCCTGAATATCTTCAGTCCAACACACTCACAGAAAAGAGTGACGTTTATAACTTTGGGGTTGTCCTGGTGGAGCTACTGACAAGTCAAAATGCAGTTCGTTCAGATAAACCCGAGGCGGAGAGAAACTTGGCCAACGTCTTTGTTTCTACAATGGAAAATAAAGATCCGCTGGATCCACTTCTTGATCCTGCAATAGTCAAGGATGGAAATCGGGAGATGATCGGAAAACTTGCCGAGCTCGCCAAAAGATGCTTAAGTGTGAAAGGAGACGACAGGCCTACCATGAGAGAAGTAGAGAGGGAGCTAGAAGAAATGCTGAGAACTTTGGCAAAGCAACCAGGAGGAAATGCCTCCTCATCGAACGAGATAGACCACTCTCTTGTTGAGGTTAGTGATGAAGTCGATGGTGGCTCTACTGGGATTAATAGCAGTGCAGAGTATGACGCCAGCATGCAGAAACAAGCCCAAAAGCCGTCCGGTTATGGTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

820

Amino Acids

90.26

Weight (kDa)

5.64

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 302 - 355 3.1e-08 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 362 - 401 4.1e-08 Calcium-binding EGF domain
Pkinase PF00069 489 - 754 2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 492 - 758 1.4e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2016
AatII GACGTC 1 cut(s) 1046
AccBSI CCGCTC 2 cut(s) 72, 116
AccI GTMKAC 1 cut(s) 1509
AclWI GGATC 8 cut(s) 12, 712, 1961, 1974, 2126, 2135, 2147, 2148
AcoI YGGCCR 3 cut(s) 1189, 1875, 2097
AcsI RAATTY 1 cut(s) 1138
AcuI CTGAAG 1 cut(s) 1964
AcyI GRCGYC 2 cut(s) 1043, 2412
AfaI GTAC 6 cut(s) 68, 228, 418, 649, 783, 1343
AfiI CCNNNNNNNGG 1 cut(s) 597
AflII CTTAAG 1 cut(s) 2221
AflIII ACRYGT 1 cut(s) 966
AgsI TTSAA 5 cut(s) 209, 1036, 1102, 1324, 1762
AjnI CCWGG 4 cut(s) 276, 1671, 2031, 2305
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AloI GAACNNNNNNTCC 2 cut(s) 1028, 1060
AluBI AGCT 8 cut(s) 18, 57, 308, 836, 866, 2041, 2206, 2272
AluI AGCT 8 cut(s) 18, 57, 308, 836, 866, 2041, 2206, 2272
Alw21I GWGCWC 2 cut(s) 59, 2208
Alw26I GTCTC 5 cut(s) 29, 1004, 1649, 1769, 2229
AlwI GGATC 8 cut(s) 12, 712, 1961, 1974, 2126, 2135, 2147, 2148
AlwNI CAGNNNCTG 2 cut(s) 836, 1778
Ama87I CYCGRG 1 cut(s) 2078
AoxI GGCC 6 cut(s) 1189, 1361, 1419, 1875, 2097, 2243
ApoI RAATTY 1 cut(s) 1138
AseI ATTAAT 1 cut(s) 2391
Asp700I GAANNNNTTC 1 cut(s) 1977
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 5 cut(s) 160, 373, 607, 1361, 1420
AsuC2I CCSGG 1 cut(s) 1214
AsuHPI GGTGA 7 cut(s) 66, 274, 460, 714, 963, 1499, 1642
AsuII TTCGAA 2 cut(s) 1179, 1901
AsuNHI GCTAGC 1 cut(s) 850
AvaI CYCGRG 1 cut(s) 2078
AvaII GGWCC 3 cut(s) 160, 373, 607
BaeI ACNNNNGTAYC 2 cut(s) 639, 672
BalI TGGCCA 2 cut(s) 1191, 2099
BamHI GGATCC 2 cut(s) 1966, 2140
BanII GRGCYC 2 cut(s) 59, 2208
BbsI GAAGAC 3 cut(s) 179, 189, 1321
Bbv12I GWGCWC 2 cut(s) 59, 2208
BccI CCATC 6 cut(s) 155, 970, 1036, 1181, 2165, 2366
BceAI ACGGC 4 cut(s) 162, 1372, 1890, 2425
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 4 cut(s) 278, 1673, 2033, 2307
BciVI GTATCC 1 cut(s) 1952
BclI TGATCA 2 cut(s) 364, 1576
BcnI CCSGG 1 cut(s) 1214
BcoDI GTCTC 5 cut(s) 29, 1004, 1649, 1769, 2229
BfaI CTAG 4 cut(s) 851, 1395, 1640, 2273
BfmI CTRYAG 3 cut(s) 654, 1434, 1510
BfrI CTTAAG 1 cut(s) 2221
BfuI GTATCC 1 cut(s) 1952
BglII AGATCT 1 cut(s) 1441
BlpI GCTNAGC 1 cut(s) 106
BmcAI AGTACT 1 cut(s) 1343
Bme1390I CCNGG 5 cut(s) 278, 1214, 1673, 2033, 2307
Bme18I GGWCC 3 cut(s) 160, 373, 607
BmeT110I CYCGRG 1 cut(s) 2078
BmgT120I GGNCC 5 cut(s) 160, 373, 607, 1361, 1420
BmiI GGNNCC 3 cut(s) 1911, 1968, 2142
BmrFI CCNGG 5 cut(s) 278, 1214, 1673, 2033, 2307
BmrI ACTGGG 2 cut(s) 572, 2394
BmsI GCATC 8 cut(s) 138, 238, 484, 572, 957, 1339, 1810, 2207
BmtI GCTAGC 1 cut(s) 854
BmuI ACTGGG 2 cut(s) 572, 2394
BpiI GAAGAC 3 cut(s) 179, 189, 1321
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 1080
Bpu1102I GCTNAGC 1 cut(s) 106
Bpu14I TTCGAA 2 cut(s) 1179, 1901
BpuEI CTTGAG 3 cut(s) 748, 873, 1430
BpuMI CCSGG 1 cut(s) 1214
Bsa29I ATCGAT 3 cut(s) 255, 453, 580
BsaBI GATNNNNATC 2 cut(s) 363, 2175
BsaHI GRCGYC 2 cut(s) 1043, 2412
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 777, 1192, 2079
BsaWI WCCGGW 2 cut(s) 328, 2444
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 79, 109
Bsc4I CCNNNNNNNGG 1 cut(s) 597
Bse118I RCCGGY 1 cut(s) 730
Bse1I ACTGG 6 cut(s) 567, 693, 1043, 1069, 1911, 2389
Bse3DI GCAATG 1 cut(s) 1057
Bse8I GATNNNNATC 2 cut(s) 363, 2175
BseBI CCWGG 4 cut(s) 278, 1673, 2033, 2307
BseCI ATCGAT 3 cut(s) 255, 453, 580
BseDI CCNNGG 3 cut(s) 777, 1192, 2079
BseGI GGATG 3 cut(s) 253, 1867, 2176
BseJI GATNNNNATC 2 cut(s) 363, 2175
BseLI CCNNNNNNNGG 1 cut(s) 597
BseMI GCAATG 1 cut(s) 1057
BseMII CTCAG 5 cut(s) 120, 133, 450, 1094, 2276
BseNI ACTGG 6 cut(s) 567, 693, 1043, 1069, 1911, 2389
BseRI GAGGAG 2 cut(s) 458, 2311
BsgI GTGCAG 2 cut(s) 1964, 2421
Bsh1285I CGRYCG 2 cut(s) 194, 958
BshFI GGCC 6 cut(s) 1191, 1363, 1421, 1877, 2099, 2245
BshVI ATCGAT 3 cut(s) 255, 453, 580
BsiEI CGRYCG 2 cut(s) 194, 958
BsiHKAI GWGCWC 2 cut(s) 59, 2208
BsiHKCI CYCGRG 1 cut(s) 2078
BsiSI CCGG 8 cut(s) 153, 329, 614, 702, 731, 1120, 1214, 2445
BslFI GGGAC 3 cut(s) 328, 993, 1963
BslI CCNNNNNNNGG 1 cut(s) 597
BsmAI GTCTC 5 cut(s) 29, 1004, 1649, 1769, 2229
BsmBI CGTCTC 1 cut(s) 2229
BsmFI GGGAC 3 cut(s) 328, 993, 1963
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 6 cut(s) 1191, 1363, 1421, 1877, 2099, 2245
Bso31I GGTCTC 1 cut(s) 29
BsoBI CYCGRG 1 cut(s) 2078
Bsp119I TTCGAA 2 cut(s) 1179, 1901
Bsp1286I GDGCHC 2 cut(s) 59, 2208
Bsp1720I GCTNAGC 1 cut(s) 106
BspANI GGCC 6 cut(s) 1191, 1363, 1421, 1877, 2099, 2245
BspCNI CTCAG 5 cut(s) 119, 132, 451, 1093, 2277
BspDI ATCGAT 3 cut(s) 255, 453, 580
BspLI GGNNCC 3 cut(s) 1911, 1968, 2142
BspMAI CTGCAG 1 cut(s) 1438
BspOI GCTAGC 1 cut(s) 854
BspPI GGATC 8 cut(s) 12, 712, 1961, 1974, 2126, 2135, 2147, 2148
BspT104I TTCGAA 2 cut(s) 1179, 1901
BspTI CTTAAG 1 cut(s) 2221
BspTNI GGTCTC 1 cut(s) 29
BsrBI CCGCTC 2 cut(s) 72, 116
BsrDI GCAATG 1 cut(s) 1057
BsrFI RCCGGY 1 cut(s) 730
BsrI ACTGG 6 cut(s) 567, 693, 1043, 1069, 1911, 2389
BssAI RCCGGY 1 cut(s) 730
BssECI CCNNGG 3 cut(s) 777, 1192, 2079
BssNI GRCGYC 2 cut(s) 1043, 2412
BssT1I CCWWGG 2 cut(s) 777, 1192
Bst2UI CCWGG 4 cut(s) 278, 1673, 2033, 2307
Bst4CI ACNGT 6 cut(s) 66, 633, 844, 1320, 1564, 1857
Bst6I CTCTTC 1 cut(s) 97
BstACI GRCGYC 2 cut(s) 1043, 2412
BstAFI CTTAAG 1 cut(s) 2221
BstAPI GCANNNNNTGC 2 cut(s) 836, 1778
BstBI TTCGAA 2 cut(s) 1179, 1901
BstC8I GCNNGC 7 cut(s) 732, 852, 1210, 1361, 2208, 2416, 2420
BstDEI CTNAG 7 cut(s) 106, 119, 347, 459, 710, 1080, 2285
BstEII GGTNACC 1 cut(s) 920
BstENI CCTNNNNNAGG 1 cut(s) 595
BstF5I GGATG 3 cut(s) 253, 1867, 2176
BstHHI GCGC 1 cut(s) 105
BstMAI GTCTC 5 cut(s) 29, 1004, 1649, 1769, 2229
BstMCI CGRYCG 2 cut(s) 194, 958
BstMWI GCNNNNNNNGC 8 cut(s) 410, 836, 863, 912, 1209, 1214, 1778, 2207
BstNI CCWGG 4 cut(s) 278, 1673, 2033, 2307
BstNSI RCATGY 3 cut(s) 44, 970, 2422
BstPI GGTNACC 1 cut(s) 920
BstSCI CCNGG 5 cut(s) 276, 1212, 1671, 2031, 2305
BstSFI CTRYAG 3 cut(s) 654, 1434, 1510
BstV2I GAAGAC 3 cut(s) 179, 189, 1321
BstX2I RGATCY 5 cut(s) 704, 1441, 1966, 2131, 2140
BstYI RGATCY 5 cut(s) 704, 1441, 1966, 2131, 2140
Bsu15I ATCGAT 3 cut(s) 255, 453, 580
BsuI GTATCC 1 cut(s) 1952
BsuRI GGCC 6 cut(s) 1191, 1363, 1421, 1877, 2099, 2245
BsuTUI ATCGAT 3 cut(s) 255, 453, 580
BtgZI GCGATG 1 cut(s) 158
BtsCI GGATG 3 cut(s) 253, 1867, 2176
BtsI GCAGTG 2 cut(s) 881, 2404
BtsIMutI CAGTG 3 cut(s) 686, 881, 2404
Cac8I GCNNGC 7 cut(s) 732, 852, 1210, 1361, 2208, 2416, 2420
CaiI CAGNNNCTG 2 cut(s) 836, 1778
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 730
Cfr13I GGNCC 5 cut(s) 160, 373, 607, 1361, 1420
ClaI ATCGAT 3 cut(s) 255, 453, 580
CseI GACGC 1 cut(s) 2420
Csp6I GTAC 6 cut(s) 67, 227, 417, 648, 782, 1342
CspCI CAANNNNNGTGG 2 cut(s) 1804, 1839
CviAII CATG 9 cut(s) 41, 377, 594, 785, 802, 967, 1116, 2251, 2419
CviQI GTAC 6 cut(s) 67, 227, 417, 648, 782, 1342
DdeI CTNAG 7 cut(s) 106, 119, 347, 459, 710, 1080, 2285
EaeI YGGCCR 3 cut(s) 1189, 1875, 2097
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
EciI GGCGGA 2 cut(s) 749, 2099
Ecl136II GAGCTC 2 cut(s) 57, 2206
Eco130I CCWWGG 2 cut(s) 777, 1192
Eco147I AGGCCT 1 cut(s) 2245
Eco24I GRGCYC 2 cut(s) 59, 2208
Eco31I GGTCTC 1 cut(s) 29
Eco32I GATATC 1 cut(s) 1087
Eco47I GGWCC 3 cut(s) 160, 373, 607
Eco53kI GAGCTC 2 cut(s) 57, 2206
Eco57I CTGAAG 1 cut(s) 1964
Eco88I CYCGRG 1 cut(s) 2078
Eco91I GGTNACC 1 cut(s) 920
EcoICRI GAGCTC 2 cut(s) 57, 2206
EcoNI CCTNNNNNAGG 1 cut(s) 595
EcoO65I GGTNACC 1 cut(s) 920
EcoRII CCWGG 4 cut(s) 276, 1671, 2031, 2305
EcoRV GATATC 1 cut(s) 1087
EcoT14I CCWWGG 2 cut(s) 777, 1192
EcoT38I GRGCYC 2 cut(s) 59, 2208
ErhI CCWWGG 2 cut(s) 777, 1192
Esp3I CGTCTC 1 cut(s) 2229
FaeI CATG 9 cut(s) 44, 380, 597, 788, 805, 970, 1119, 2254, 2422
FalI AAGNNNNNCTT 2 cut(s) 1135, 1167
FaqI GGGAC 3 cut(s) 328, 993, 1963
FatI CATG 9 cut(s) 40, 376, 593, 784, 801, 966, 1115, 2250, 2418
FauI CCCGC 3 cut(s) 406, 691, 1352
FbaI TGATCA 2 cut(s) 364, 1576
FblI GTMKAC 1 cut(s) 1509
FokI GGATG 3 cut(s) 260, 1874, 2183
FriOI GRGCYC 2 cut(s) 59, 2208
FspBI CTAG 4 cut(s) 851, 1395, 1640, 2273
GlaI GCGC 1 cut(s) 104
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 6 cut(s) 1191, 1363, 1421, 1877, 2099, 2245
HapII CCGG 8 cut(s) 153, 329, 614, 702, 731, 1120, 1214, 2445
HgaI GACGC 1 cut(s) 2420
HhaI GCGC 1 cut(s) 105
Hin1I GRCGYC 2 cut(s) 1043, 2412
Hin1II CATG 9 cut(s) 44, 380, 597, 788, 805, 970, 1119, 2254, 2422
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 3 cut(s) 427, 455, 1406
HpaII CCGG 8 cut(s) 153, 329, 614, 702, 731, 1120, 1214, 2445
HphI GGTGA 7 cut(s) 66, 274, 460, 714, 963, 1499, 1642
Hpy166II GTNNAC 4 cut(s) 11, 550, 1510, 1678
Hpy188I TCNGA 9 cut(s) 29, 54, 369, 460, 559, 1569, 1888, 2071, 2192
Hpy8I GTNNAC 4 cut(s) 11, 550, 1510, 1678
Hpy99I CGWCG 3 cut(s) 633, 746, 1922
HpyAV CCTTC 4 cut(s) 173, 364, 1166, 1484
HpyCH4III ACNGT 6 cut(s) 66, 633, 844, 1320, 1564, 1857
HpyCH4IV ACGT 4 cut(s) 1043, 1130, 2010, 2103
HpyF10VI GCNNNNNNNGC 8 cut(s) 410, 836, 863, 912, 1209, 1214, 1778, 2207
HpyF3I CTNAG 7 cut(s) 106, 119, 347, 459, 710, 1080, 2285
HpySE526I ACGT 4 cut(s) 1043, 1130, 2010, 2103
Hsp92I GRCGYC 2 cut(s) 1043, 2412
Hsp92II CATG 9 cut(s) 44, 380, 597, 788, 805, 970, 1119, 2254, 2422
HspAI GCGC 1 cut(s) 103
KroI GCCGGC 1 cut(s) 730
KroNI GCCGGC 1 cut(s) 732
Ksp22I TGATCA 2 cut(s) 364, 1576
LmnI GCTCC 5 cut(s) 15, 54, 62, 2038, 2269
LweI GCATC 8 cut(s) 138, 238, 484, 572, 957, 1339, 1810, 2207
MaeI CTAG 4 cut(s) 851, 1395, 1640, 2273
MaeII ACGT 4 cut(s) 1043, 1130, 2010, 2103
MaeIII GTNAC 5 cut(s) 296, 920, 962, 969, 2006
MbiI CCGCTC 2 cut(s) 72, 116
MfeI CAATTG 1 cut(s) 1388
MflI RGATCY 5 cut(s) 704, 1441, 1966, 2131, 2140
MhlI GDGCHC 2 cut(s) 59, 2208
MlsI TGGCCA 2 cut(s) 1191, 2099
MluCI AATT 8 cut(s) 323, 930, 1012, 1138, 1239, 1388, 1482, 1821
MluNI TGGCCA 2 cut(s) 1191, 2099
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 5 cut(s) 495, 1839, 1866, 1958, 2010
Mox20I TGGCCA 2 cut(s) 1191, 2099
MroNI GCCGGC 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 1977
MscI TGGCCA 2 cut(s) 1191, 2099
MseI TTAA 6 cut(s) 272, 510, 1142, 1587, 2222, 2391
MslI CAYNNNNRTG 2 cut(s) 1623, 1833
Msp20I TGGCCA 2 cut(s) 1191, 2099
MspA1I CMGCKG 4 cut(s) 100, 836, 960, 2137
MspCI CTTAAG 1 cut(s) 2221
MspI CCGG 8 cut(s) 153, 329, 614, 702, 731, 1120, 1214, 2445
MspR9I CCNGG 5 cut(s) 278, 1214, 1673, 2033, 2307
MunI CAATTG 1 cut(s) 1388
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 4 cut(s) 278, 1673, 2033, 2307
MwoI GCNNNNNNNGC 8 cut(s) 410, 836, 863, 912, 1209, 1214, 1778, 2207
NaeI GCCGGC 1 cut(s) 732
NciI CCSGG 1 cut(s) 1214
NgoMIV GCCGGC 1 cut(s) 730
NheI GCTAGC 1 cut(s) 850
NlaIII CATG 9 cut(s) 44, 380, 597, 788, 805, 970, 1119, 2254, 2422
NlaIV GGNNCC 3 cut(s) 1911, 1968, 2142
NmeAIII GCCGAG 1 cut(s) 2227
NmuCI GTSAC 2 cut(s) 969, 2006
NspI RCATGY 3 cut(s) 44, 970, 2422
NspV TTCGAA 2 cut(s) 1179, 1901
PaeI GCATGC 1 cut(s) 2422
PceI AGGCCT 1 cut(s) 2245
PciI ACATGT 1 cut(s) 966
PcsI WCGNNNNNNNCGW 1 cut(s) 1131
PctI GAATGC 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 732
PdmI GAANNNNTTC 1 cut(s) 1977
PfeI GAWTC 2 cut(s) 455, 1406
Ple19I CGATCG 1 cut(s) 194
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
PscI ACATGT 1 cut(s) 966
PshBI ATTAAT 1 cut(s) 2391
PsiI TTATAA 1 cut(s) 2016
Psp124BI GAGCTC 2 cut(s) 59, 2208
Psp6I CCWGG 4 cut(s) 276, 1671, 2031, 2305
PspEI GGTNACC 1 cut(s) 920
PspGI CCWGG 4 cut(s) 276, 1671, 2031, 2305
PspN4I GGNNCC 3 cut(s) 1911, 1968, 2142
PspPI GGNCC 5 cut(s) 160, 373, 607, 1361, 1420
PstI CTGCAG 1 cut(s) 1438
PstNI CAGNNNCTG 2 cut(s) 836, 1778
PsuI RGATCY 5 cut(s) 704, 1441, 1966, 2131, 2140
PvuI CGATCG 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 6 cut(s) 68, 228, 418, 649, 783, 1343
RsaNI GTAC 6 cut(s) 67, 227, 417, 648, 782, 1342
RseI CAYNNNNRTG 2 cut(s) 1623, 1833
SacI GAGCTC 2 cut(s) 59, 2208
SaqAI TTAA 6 cut(s) 272, 510, 1142, 1587, 2222, 2391
Sau96I GGNCC 5 cut(s) 160, 373, 607, 1361, 1420
ScaI AGTACT 1 cut(s) 1343
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 5 cut(s) 278, 1214, 1673, 2033, 2307
SduI GDGCHC 2 cut(s) 59, 2208
SfaNI GCATC 8 cut(s) 138, 238, 484, 572, 957, 1339, 1810, 2207
SfcI CTRYAG 3 cut(s) 654, 1434, 1510
SfuI TTCGAA 2 cut(s) 1179, 1901
SinI GGWCC 3 cut(s) 160, 373, 607
SmiMI CAYNNNNRTG 2 cut(s) 1623, 1833
SmlI CTYRAG 4 cut(s) 763, 888, 1445, 2221
SmoI CTYRAG 4 cut(s) 763, 888, 1445, 2221
SphI GCATGC 1 cut(s) 2422
Sse9I AATT 8 cut(s) 323, 930, 1012, 1138, 1239, 1388, 1482, 1821
SseBI AGGCCT 1 cut(s) 2245
SspI AATATT 2 cut(s) 490, 1368
SspMI CTAG 4 cut(s) 851, 1395, 1640, 2273
SstI GAGCTC 2 cut(s) 59, 2208
StuI AGGCCT 1 cut(s) 2245
StyD4I CCNGG 5 cut(s) 276, 1212, 1671, 2031, 2305
StyI CCWWGG 2 cut(s) 777, 1192
TaaI ACNGT 6 cut(s) 66, 633, 844, 1320, 1564, 1857
TaiI ACGT 4 cut(s) 1046, 1133, 2013, 2106
TasI AATT 8 cut(s) 323, 930, 1012, 1138, 1239, 1388, 1482, 1821
TatI WGTACW 2 cut(s) 226, 1341
TauI GCSGC 4 cut(s) 113, 116, 129, 1421
TfiI GAWTC 2 cut(s) 455, 1406
Tru1I TTAA 6 cut(s) 272, 510, 1142, 1587, 2222, 2391
Tru9I TTAA 6 cut(s) 272, 510, 1142, 1587, 2222, 2391
TscAI CASTG 3 cut(s) 693, 888, 2404
TseFI GTSAC 2 cut(s) 969, 2006
Tsp45I GTSAC 2 cut(s) 969, 2006
TspGWI ACGGA 3 cut(s) 28, 1046, 1140
TspRI CASTG 3 cut(s) 693, 888, 2404
Vha464I CTTAAG 1 cut(s) 2221
VpaK11BI GGWCC 3 cut(s) 160, 373, 607
VspI ATTAAT 1 cut(s) 2391
XagI CCTNNNNNAGG 1 cut(s) 595
XapI RAATTY 1 cut(s) 1138
XceI RCATGY 3 cut(s) 44, 970, 2422
XcmI CCANNNNNNNNNTGG 2 cut(s) 154, 1035
XmiI GTMKAC 1 cut(s) 1509
XmnI GAANNNNTTC 1 cut(s) 1977
XspI CTAG 4 cut(s) 851, 1395, 1640, 2273
ZraI GACGTC 1 cut(s) 1044
ZrmI AGTACT 1 cut(s) 1343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.